CN204594998U - A kind of Coxiella burnetii antibody assay kit - Google Patents

A kind of Coxiella burnetii antibody assay kit Download PDF

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Publication number
CN204594998U
CN204594998U CN201520270972.2U CN201520270972U CN204594998U CN 204594998 U CN204594998 U CN 204594998U CN 201520270972 U CN201520270972 U CN 201520270972U CN 204594998 U CN204594998 U CN 204594998U
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China
Prior art keywords
test paper
chromatography test
immune chromatography
upper shell
coxiella burnetii
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Expired - Fee Related
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CN201520270972.2U
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Chinese (zh)
Inventor
杨宇
王静
徐宝梁
赵婷婷
王建成
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Chinese Academy of Inspection and Quarantine CAIQ
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Chinese Academy of Inspection and Quarantine CAIQ
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Abstract

The utility model discloses a kind of Coxiella burnetii antibody assay kit, comprise the immune chromatography test paper, upper shell and the lower house that detect Coxiella burnetii antibody; Described upper shell and described lower house fasten mutually, and described immune chromatography test paper is placed in described lower house; Corresponding to described immune chromatography test paper, offer well, detection window and bleeder vent at described upper shell upper surface; Described immune chromatography test paper is the multi-layer film structure with drying agent bottom.The Coxiella burnetii antibody assay kit that the utility model provides, structural design is simple, cost is low, lightly, be easy to operation, be convenient to the preparation preparing Coxiella burnetii antibody test reagent, and the immune chromatography test paper detecting Coxiella burnetii antibody can be stablized and is positioned in housing, the immune chromatography test paper moisture absorption can not be made along with reagent is in transportation, to come off or position is departed from, effectively avoid inaccurate, the incredible thing of testing result to occur.

Description

A kind of Coxiella burnetii antibody assay kit
Technical field
The utility model relates to detection reagent, in particular to a kind of Coxiella burnetii antibody assay kit.
Background technology
Q heat is a kind of Amphixenosis common in the world, and its pathogen is Coxiella burnetii (Coxiella burnetii, Cb).Coxiella burnetii is the entozoic microorganism of special sexual cell, breeds in host cell phagolysosome.Whether once being infected by Coxiella burnetii, carrying out examination by detecting Coxiella burnetii antibody.
Existing quick screening method is mainly the examination of Coxiella burnetii antibody immune chromatography test paper.This test paper is applicable to field quick detection, and common laboratory is left in the exsiccator with silica-gel desiccant after preparing Coxiella burnetii antibody immune chromatography Test paper.This test paper, in transportation or in the process deposited, easily makes moist, unstable, and testing result finally can be caused inaccurate.Sometimes, also can be improper because of the preservation of test paper, there is cross pollution, cause testing result insincere.
The packaging of existing immune chromatography test paper, the aluminium-foil paper packaging that general employing is independent, and drying agent is added with in single aluminium-foil paper.During use, need to tear aluminium-foil paper packaging, first process wrapping paper and drying agent, just can detect, operate not convenient; And, deal with improperly and easily cause cross-infection.
Summary of the invention
The purpose of this utility model is to provide a kind of Coxiella burnetii antibody assay kit, with problems such as inaccurate, the easy pollutions solving that existing Coxiella burnetii antibody test reagent may cause depositing, in transportation.
The utility model embodiment provides a kind of Coxiella burnetii antibody assay kit, comprises the immune chromatography test paper, upper shell and the lower house that detect Coxiella burnetii antibody; Described upper shell and described lower house fasten mutually, and described immune chromatography test paper is placed in described lower house; Corresponding to described immune chromatography test paper, offer well, detection window and bleeder vent at described upper shell upper surface; Described immune chromatography test paper is the multi-layer film structure with drying agent bottom.
In certain embodiments, be preferably, described immune chromatography test paper comprises base plate, drying agent, sample pad, pad, chromatographic film and adsorptive pads, the lower surface of described base plate is pasted with described drying agent, pastes described sample pad, pad, chromatographic film and adsorptive pads with overlapping successively at the upper surface of described base plate; Described chromatographic film surface is provided with the detection line and nature controlling line that are parallel to each other.
In certain embodiments, be preferably, described well and air hole are the through hole running through described housing upper surface, correspond to the sample pad of described immune chromatography test paper immediately below described well, the adsorptive pads of corresponding described immune chromatography test paper immediately below described air hole, described detection window is through hole; The detection line of corresponding described immune chromatography test paper and nature controlling line immediately below described detection window.
In certain embodiments, be preferably, described lower house inside is provided with the fixture can fixing immune chromatography test paper, and described fixture is elastic memory sheet, its one end is fixedly connected on the inwall of described lower house, and the other end is rotatable and push down described immune chromatography test paper.
In certain embodiments, be preferably, described upper shell is also provided with mobilizable gland bonnet, and described gland bonnet covers described well, detection window and bleeder vent.
In certain embodiments, be preferably, described gland bonnet is push-and-pull lid, and this push-and-pull lid can be fastened on described upper shell, covers described well, detection window and bleeder vent.
In certain embodiments, be preferably, described gland bonnet is swivelling cover, is fixedly connected on housing by the vertical rotating shaft be arranged on described upper shell, and bonded to each other with housing, and described swivelling cover can with vertical rotating shaft for axle horizontally rotates relative to surface of shell.
In certain embodiments, be preferably, described gland bonnet is set to Split type structure, and its part is for sealing described well, and another part is for sealing described detection window and bleeder vent.
In certain embodiments, be preferably, the outside surface of described gland bonnet is matte surface.
In certain embodiments, be preferably, described upper shell be also provided with the groove placing suction pipe.
A kind of Coxiella burnetii antibody assay kit that the utility model provides, this detection kit is on the basis of existing Coxiella burnetii antibody immune chromatography test paper, be equipped with drying agent bottom, effectively can prevent the phenomenon of the moisture absorption, avoid immune chromatography test paper to lose efficacy because of the moisture absorption.Immune chromatography test paper is placed in the middle of housing, effectively can avoids the cross pollution that misoperation may cause.
This Coxiella burnetii antibody assay kit, structural design is simple, cost is low, lightly, be easy to operation, be convenient to the preparation of Coxiella burnetii antibody test reagent, and the immune chromatography test paper detecting Coxiella burnetii antibody can be stablized and is positioned in housing, the immune chromatography test paper moisture absorption can not be made along with reagent is in transportation, to come off or position is departed from, effectively avoid testing result inaccurate, insincere.
Use to increase and have the Coxiella burnetii antibody assay kit of gland bonnet, when detecting, easy and simple to handle and be convenient to mark, operating process can not produce discarded object, avoiding a large amount of sample when detecting, delaying because of process discarded object, effectively increasing work efficiency.
Accompanying drawing explanation
Fig. 1 is the profile structural representation of the utility model one preferred embodiment immune chromatography test paper.
Fig. 2 is the structural representation of the utility model one preferred embodiment upper shell.
Fig. 3 is the structural representation of the utility model one preferred embodiment upper shell.
Fig. 4 is the structural representation of the utility model one preferred embodiment upper shell.
Embodiment
It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can combine mutually.Please refer to accompanying drawing below and describe the utility model in detail in conjunction with the embodiments.
Consider the Coxiella burnetii antibody immune chromatography detection paper reagent adopted at present, on the one hand in packed and transported process, easily make moist; On the other hand, the inconvenience of dress of unpacking in use, very easily cross pollution occurs, cause testing result inaccurate, insincere, the utility model provides a kind of Coxiella burnetii antibody assay kit.
This Coxiella burnetii antibody assay kit, comprises the immune chromatography test paper, upper shell and the lower house that detect Coxiella burnetii antibody; Described upper shell and described lower house fasten mutually, and described immune chromatography test paper is placed in described lower house; Corresponding to described immune chromatography test paper, offer well at described upper shell upper surface, detection window and bleeder vent; Described immune chromatography test paper is the multi-layer film structure with drying agent bottom.
Detecting the immune chromatography test paper of Coxiella burnetii antibody, bottom is equipped with drying agent, makes this immune chromatography test paper in transportation, will immune chromatography test paper be caused to lose efficacy because of the moisture absorption.The immune chromatography test paper of Coxiella burnetii antibody is placed in housing, makes immune chromatography test paper can not moving or rocking because of detection kit, and come off or depart from; The setting of bleeder vent, makes immune chromatography test paper in the housing, and after avoiding causing adding sample because of the sealing of housing, sample fast to adsorptive pads diffusion, can not affect testing result.Housing the cross pollution can effectively avoided because misoperation causes is set.
Next, the utility model will describe this Coxiella burnetii antibody assay kit in detail by some specific embodiments.
Embodiment 1
Coxiella burnetii antibody assay kit, comprise the immune chromatography test paper, upper shell and the lower house that detect Coxiella burnetii antibody, upper shell and lower house fasten mutually, and described immune chromatography test paper is placed in described lower house.
The immune chromatography test paper of detection Coxiella burnetii antibody is the multi-layer film structure with drying agent bottom.Particularly, as shown in Figure 1, this immune chromatography test paper is the multi-layer film structure with drying agent bottom 1, comprises base plate 2, and pastes sample pad 3, pad 4, chromatographic film 5 and adsorptive pads 6 successively with overlapping at the upper surface of base plate 2; Sample pad 3, pad 4, to be closely connected between chromatographic film 5 and each tunic of these membrane structures of adsorptive pads 6, overlapping at least partly; Chromatographic film 5 is coated with the detection line 7 and nature controlling line 8 that are parallel to each other, and detection line is near pad side, and nature controlling line is close adsorptive pads side away from pad; The lower surface of described base plate 2 is pasted with drying agent 1, and drying agent can adopt fiber desiccant.
Further, immune chromatography test paper is strip, and sample pad 3 can adopt glass film or polyester film, and its front end is pasted on base plate front end, and end lap is on pad 4; Pad 4 comprises film matrix, and be evenly coated on this film matrix surface be fixed with the coating that staphylococcal protein A (SPA) marks magnetic nanoparticle or colloid gold particle, pad 4 is between sample pad 3 and chromatographic film 5, its front end overlap joint is pasted on base plate 2, and certain length is extended in rear end above chromatographic film 5 front end; The front end of chromatographic film 5 to be inserted between pad 4 and base plate 2 and to be bonded on base plate 2; Adsorptive pads 6 is overlapped in chromatographic film 5, and is pasted on the rear end of base plate 2; This chromatographic film 5 is provided with parallel detection line 7 and nature controlling line 8.Detection line 7 is near pad 4, and detection line adopts the coating of Coxiella burnetii antigen, and nature controlling line 8 is near adsorptive pads 6, and nature controlling line adopts the antibody that can be combined with SPA to apply, and pad can adopt glass film or polyester film, and chromatographic film can adopt nitrocellulose filter.
Be positioned in lower house by the immune chromatography test paper of the detection Coxiella burnetii antibody of above-mentioned preparation, upper shell is fastened on lower house.Upper shell is arranged the appropriate location corresponding to immune chromatography test paper and be provided with well, detection window and bleeder vent.Particularly, as shown in Figure 2, well 10 and bleeder vent 12, for running through the through hole of upper shell 9, correspond to the sample pad of above-mentioned immune chromatography test paper immediately below well 10, the adsorptive pads of corresponding above-mentioned immune chromatography test paper immediately below bleeder vent 12, and detecting 11 is also through hole; The detection line of corresponding above-mentioned immune chromatography test paper and nature controlling line immediately below detection window 11.Detection window also can be set to transparent form, just refers to be coated with layer of transparent film on through hole.
Embodiment 2
Consider that immunochromatography reagent is when assembling, and is convenient to the immune chromatography test paper of fixed test Coxiella burnetii antibody, easy to assembly, be provided with in lower house inside the fixture can fixing immune chromatography test paper.This fixture can adopt elastic memory sheet, and its one end is fixedly connected on the inwall of lower house, and the other end is rotatable and push down described immune chromatography test paper.
Embodiment 3
In transport, storing process, in order to avoid polluting, causing test strips inefficacy or testing result to occur deviation, upper shell is provided with gland bonnet.Under normality, sealing lid covers well, detection window and bleeder vent.During detection, gland bonnet is opened, expose well, detection window and bleeder vent.That is sealing lid is that activity is arranged, and can open or cover well, detection window and bleeder vent.
Gland bonnet is established and can be adopted push-and-pull lid, adopts plug-type design, is fastened on housing, and by well, detection window and bleeder vent cover.Particularly, upper and lower casing is rectangle, and push-and-pull lid is rectangle, and push-and-pull lid is fastened on upper shell, and about push-and-pull lid, two long limits are provided with the outward flange that can engage with upper shell.Further, come off to prevent push-and-pull lid, in the housing outer surface left and right sides, offer the push-and-pull groove for engaging push-and-pull lid, can at the outward flange of push-and-pull lid behind front end or the position of rear end, projection is provided with towards card, projection is fastened in push-and-pull groove, push open or drawing push-and-pull lid time, because projection is slided in push-and-pull groove, push-and-pull lid can not be pulled, also would not make to push away to cover to depart from housing.
As required, the setting of gland bonnet can be wholely set, and split also can be adopted to arrange.As shown in Figure 3, when split is arranged, be divided into two gland bonnets, first gland bonnet 13 is for sealing well 10, and the second gland bonnet 131 is for sealing detection window 11 and bleeder vent 12.Two, the left and right edge card of two gland bonnets is combined in upper shell both sides, in order to avoid gland bonnet is not pulled, is all provided with push-and-pull groove 14 in upper shell both sides; On gland bonnet 13 and 131, be provided with the edge of engaging upper shell, be provided with projection in the one end in the middle of housing, this projection and push-and-pull groove 14 are mutually adaptive.Like this, when pushing gland bonnet open, the projection on gland bonnet can be slided in push-and-pull groove, and is finally stuck in the initiating terminal of groove, gland bonnet can not be pulled, and ensures gland bonnet not slippage further.After detection kit uses, can, by gland bonnet pushed home, detection sample be sealed in detection kit, can not cause and other cross pollution detecting reagent, use reliable, safety.
Embodiment 4
In the present embodiment, the gland bonnet in embodiment 3 is replaced with swivelling cover.Swivelling cover, by being fixedly connected with the vertical rotating shaft be arranged on above upper shell outside surface, realizes with housing bonded to each other, and rotatable dislocation.At ordinary times, swivelling cover covers above upper shell, well, detection window and bleeder vent is covered; When detecting, by swivelling cover with vertical rotating shaft for axle, corresponding surface of shell horizontally rotates, and can expose well, detection window and bleeder vent.
As required, above-mentioned gland bonnet can be set to two, one for covering well, another is for covering detection window and bleeder vent.
Gland bonnet in above-described embodiment, its outside surface can be used for making marks, the information of mark sample or numbering etc., but as adopted smooth surface, marking and easily being erased, in order to prevent mark from erasing, outer surface of outer cover being set to matte surface in operating process.
Embodiment 5
In order to detect the convenience of sample, the suction pipe of pipette samples need not being configured for separately, when assembling kit, on the upper shell of above-described embodiment, the groove placing suction pipe can being provided with.As shown in Figure 4, groove 17 is at well 10, and the side of detection window 11 and bleeder vent 12 is arranged, the placement location being namely parallel to immune chromatography test paper is arranged, groove size is just in time suitable with suction pipe, by suction pipe engaging in a groove, is convenient to the use of Site Detection reagent.
The above, it is only preferred embodiment of the present utility model, be not restriction the utility model being done to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solutions of the utility model content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present utility model, still belong to the protection domain of technical solutions of the utility model.

Claims (10)

1. a Coxiella burnetii antibody assay kit, is characterized in that, comprises the immune chromatography test paper, upper shell and the lower house that detect Coxiella burnetii antibody; Described upper shell and described lower house fasten mutually, and described immune chromatography test paper is placed in described lower house; Corresponding to described immune chromatography test paper, offer well, detection window and bleeder vent at described upper shell upper surface; Described immune chromatography test paper is the multi-layer film structure with drying agent bottom.
2. detection kit as claimed in claim 1, it is characterized in that, described immune chromatography test paper comprises base plate, drying agent, sample pad, pad, chromatographic film and adsorptive pads, the lower surface of described base plate is pasted with described drying agent, pastes described sample pad, pad, chromatographic film and adsorptive pads with overlapping successively at the upper surface of described base plate; Described chromatographic film surface is provided with the detection line and nature controlling line that are parallel to each other.
3. detection kit as claimed in claim 2, it is characterized in that, described well and air hole are the through hole running through described housing upper surface, the sample pad of described immune chromatography test paper is corresponded to immediately below described well, the adsorptive pads of corresponding described immune chromatography test paper immediately below described air hole, described detection window is through hole; The detection line of corresponding described immune chromatography test paper and nature controlling line immediately below described detection window.
4. detection kit as claimed in claim 1, it is characterized in that, described lower house inside is provided with the fixture can fixing immune chromatography test paper, described fixture is elastic memory sheet, its one end is fixedly connected on the inwall of described lower house, and the other end is rotatable and push down described immune chromatography test paper.
5. detection kit as claimed in claim 1, it is characterized in that, described upper shell is also provided with mobilizable gland bonnet, and described gland bonnet covers described well, detection window and bleeder vent.
6. detection kit as claimed in claim 5, it is characterized in that, described gland bonnet is push-and-pull lid, and this push-and-pull lid can be fastened on described upper shell, covers described well, detection window and bleeder vent.
7. detection kit as claimed in claim 5, it is characterized in that, described gland bonnet is swivelling cover, is fixedly connected on housing by the vertical rotating shaft be arranged on described upper shell, and bonded to each other with housing, described swivelling cover can with vertical rotating shaft for axle horizontally rotates relative to surface of shell.
8., as the detection kit as described in arbitrary in claim 5-7, it is characterized in that, described gland bonnet is set to Split type structure, and its part is for sealing described well, and another part is for sealing described detection window and bleeder vent.
9. detection kit as claimed in claim 5, it is characterized in that, the outside surface of described gland bonnet is matte surface.
10. detection kit as claimed in claim 1, is characterized in that, described upper shell is also provided with the groove placing suction pipe.
CN201520270972.2U 2015-04-29 2015-04-29 A kind of Coxiella burnetii antibody assay kit Expired - Fee Related CN204594998U (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105973888A (en) * 2016-07-21 2016-09-28 韩雅君 Kit for rapidly detecting ischemia modified albumin in blood
CN105973887A (en) * 2016-07-21 2016-09-28 韩雅君 Chip for rapidly detecting ischemia modified albumin in blood
CN106771176A (en) * 2016-11-18 2017-05-31 百奥森(江苏)食品安全科技有限公司 A kind of avian influenza virus antigen rapid detection card
CN109085159A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of substrate and detection card
CN110702913A (en) * 2019-10-28 2020-01-17 中国人民解放军军事科学院军事医学研究院 Monoclonal antibody composition for quantitatively detecting coxiella burnetii I strain
CN112305217A (en) * 2020-11-19 2021-02-02 深圳市易瑞生物技术股份有限公司 Split type immunochromatography detection device and immunochromatography detection method
CN117129671A (en) * 2023-10-26 2023-11-28 苏州星安生物科技有限公司 Excrement sample collection and detection integrated device

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105973888A (en) * 2016-07-21 2016-09-28 韩雅君 Kit for rapidly detecting ischemia modified albumin in blood
CN105973887A (en) * 2016-07-21 2016-09-28 韩雅君 Chip for rapidly detecting ischemia modified albumin in blood
CN105973888B (en) * 2016-07-21 2018-09-18 西安良升生物科技有限公司 The kit of ischemia modified albumin IMA in a kind of quick detection blood
CN105973887B (en) * 2016-07-21 2018-09-18 西安良升生物科技有限公司 The chip of ischemia modified albumin IMA in a kind of quick detection blood
CN106771176A (en) * 2016-11-18 2017-05-31 百奥森(江苏)食品安全科技有限公司 A kind of avian influenza virus antigen rapid detection card
CN109085159A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of substrate and detection card
CN109085159B (en) * 2017-08-21 2024-09-06 上海荒岛科技有限公司 Base plate and detection card
CN110702913A (en) * 2019-10-28 2020-01-17 中国人民解放军军事科学院军事医学研究院 Monoclonal antibody composition for quantitatively detecting coxiella burnetii I strain
CN110702913B (en) * 2019-10-28 2023-03-31 中国人民解放军军事科学院军事医学研究院 Monoclonal antibody composition for quantitatively detecting coxiella burnetii I strain
CN112305217A (en) * 2020-11-19 2021-02-02 深圳市易瑞生物技术股份有限公司 Split type immunochromatography detection device and immunochromatography detection method
CN117129671A (en) * 2023-10-26 2023-11-28 苏州星安生物科技有限公司 Excrement sample collection and detection integrated device
CN117129671B (en) * 2023-10-26 2024-01-23 苏州星安生物科技有限公司 Excrement sample collection and detection integrated device

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