CN105969510A - High-oil yield preparation method of grape seed oil - Google Patents
High-oil yield preparation method of grape seed oil Download PDFInfo
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- CN105969510A CN105969510A CN201610328727.1A CN201610328727A CN105969510A CN 105969510 A CN105969510 A CN 105969510A CN 201610328727 A CN201610328727 A CN 201610328727A CN 105969510 A CN105969510 A CN 105969510A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/06—Production of fats or fatty oils from raw materials by pressing
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/001—Refining fats or fatty oils by a combination of two or more of the means hereafter
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- Oil, Petroleum & Natural Gas (AREA)
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Abstract
The invention discloses a high-oil yield preparation method of grape seed oil. The preparation method comprises the following steps: (1) impurity removal; (2) soaking; (3) warm boiling; (4) pretreatment; (5) peeling; (6) screening; (7) low-temperature grinding; (8) enzymolysis; (9) drying; (10) squeezing; and (11) purification. In the method disclosed by the invention, the collocation is scientific and reasonable, the overall energy consumption is low, and the cost is low; without introducing chemical substances, the safety of the grape seed oil is guaranteed, the content of harmful substances in the oil is reduced, and the condition and mouthfeel of the oil are improved; and moreover, the final oil yield of the grape seeds can be kept over 98%, thereby bringing great economic benefits and popularization values.
Description
Technical field
The invention belongs to food processing technology field, be specifically related to the preparation method of a kind of Oleum Vitis viniferae high oil recovery.
Background technology
The garbage (skin, seed, stalk) that wine production process produces accounts for the 20%-30% of fresh fruit total amount, and wherein Semen Vitis viniferae accounts for the 7%-10% of fresh fruit gross weight, and the Semen Vitis viniferae garbage that China's brewing industry produces every year is more than 4.2 ten thousand tons.Northern Xinjiang Northern Slope of Tianshan Mountains and east boundary Turfan, Hami Prefecture abound with vinifera, and Wine Enterprises is more, stone member 50,000 mu of viniferas of achievement, are just having 40,000 mu at trophophase, are being planned for 2015 cultivated area expands to 200,000 mu.At present, Semen Vitis viniferae is in addition to small part is as feedstuff and fertilizer, and major part is simply discarded, and not only causes the waste of resource, too increases ambient pressure.
Research finds, it is suitable with main oil crop Semen sojae atricolor oil content that the oil content of Semen Vitis viniferae is about 14%-17%(), belong to the semi-drying oils and fats of unsaturated fatty acid, containing a large amount of unsaturated fatty acids, content is up to more than 90%, including Palmic acid, stearic acid, oleic acid, linoleic acid and trace linolenic acid, lauric acid, myristic acid etc..Its main function composition is linoleic acid, for essential fatty acid, content reaches more than 70%, the highest than in general edible oil even medicinal oil walnut oil Flos Carthami oil of content, it is one of important composition constituting human body cell film and skin, for child's brain and neurodevelopment, and maintaining the blood fat of adult to balance, reduce cholesterol, prevent thrombosis, prevention of arterial hardening etc. acts on and all playing an important role.In addition, Oleum Vitis viniferae is possibly together with a small amount of (≤1%) linolenic acid, linolenic acid is all essential fatty acid, must be taken in by diet, can be EPA(eicosapentaenoic acid by liver metabolism), DHA(docosahexenoic acid) and DPA(clupanodonic acid) 3 kinds of polyunsaturated fatty acid, be there is the characteristic easily being entered brain cell by blood vessel, it it is the brain-invigorating nutrition of high-quality, nervous system, brain and amphiblestroid growth can be promoted, improve brain function and learning and memory, can play and improve memory, prevention senile dementia and anaphylaxis effect.Oleum Vitis viniferae contains multiple fatsoluble vitamin (A, E, D, K, P) and various trace elements (Ca, Fe, Zn, Mn, Cu).Additionally, Oleum Vitis viniferae contains procyanidin (OPC), it is that current nature finding, antioxidation, aging resistance, offer acid-base balance, material that Scavenging ability is the strongest, prevention of various diseases occur, particularly have good preventive effect to cardiovascular and cerebrovascular disease.Oleum Vitis viniferae has the effect such as improvement result and regulation immunity of organisms, antiallergic, antiinflammatory to microcirculation in human body, Patients with Cardiovascular/Cerebrovascular Diseases and renal disease patient there is auxiliary therapeutic action, can also blood circulation promoting, make fat gradually cut down, play effect of weight-reducing.Oleum Vitis viniferae nutritive value and medical function all obtain the abundant affirmation of domestic and international medical circle and nutritionist.
Tradition extracts the major way of Oleum Vitis viniferae solvent extraction method, supercritical carbon dioxide fluid extraction, mechanical expression method, aqueous enzymatic method.Although solvent extraction method extraction efficiency easy and simple to handle is higher, but demonstrating industry shortcoming, dissolvent residual can bring food safety hidden danger, high temperature extraction environmental disruption effective ingredient；Supercritical carbon dioxide fluid extraction extraction efficiency is high, and extraction product is pollution-free, remains bioactive functions composition, but owing to material requested, apparatus expensive make extraction cost high, is therefore mainly focused on small scale experiments, it is more difficult to realize large-scale industrial production.Aqueous enzymatic method prepares Oleum Vitis viniferae can improve oil yield, but owing to enzyme consumption is big, cost is significantly increased；Mechanical expression method, due to device simple, easy and simple to handle, easily realizes industrialization large-scale production and is favored by brewery, but conventional press method is carried out under 105 DEG C of high temperature, easily makes active component and thermally labile component destroyed by decomposition.
In view of to the demand of pollution-free food and growing with each passing day of environmental consciousness, cold pressing oil technology, to reduce the commercial production pollution to product and environment as advantage, causes concern greatly producing special health oils and fats field.
Cold pressing oil belongs to physics liquefaction method, keep having the following characteristics that 1 by mechanical force material pressurizeed this technique under conditions of temperature constant) avoid the high temperature impact on material, the harmful substance such as trans fatty acid, oils and fats polymer will not be produced in expressing process and make nutrition of lipids material degeneration, remain the active substance in expressed oil；2) pressed oil obtained meets the most edible condition, need not further oil and fat refining, thus avoid the stability that pressed oil directly contacts with alkali liquor, bleaching clay, phosphoric acid etc. in refining process and oils and fats is likely to result in by high temperature during refine and destroy, remain the peculiar flavour of various fat-soluble nutrients compositions such as VA and VE oils and fats in oils and fats to greatest extent；3) eliminate while all kinds of industrial chemicals such as soda acid consume, it is to avoid Fructus Gleditsia, soil wash the pollution to environment of the various reaction waste such as waste water, and therefore cold pressing process is a kind of oil-producing technique having fewer environmental impacts, pollution-free at whole course of processing Green；4) Oleum Vitis viniferae extracted with cold-press has natural pollution-free, rich in fat-soluble nutrients composition and natural flavour mountaineous advantage, meets edible natural organic pollution-free food theory that current people are praised highly.
Although cold-squeezing also exists plurality of advantages, it is ensured that the safety of food, but also there is drawback.Common cold pressing only carries out strip plate process to oil embryo, do not carry out special handling, cause oilseed cell wall damage incomplete, cause oily residual rate too high, and cold pressing uses the mode that constant voltage leaches, extraction efficiency is the highest, in addition, traditional oil plant seed coat, residue etc. can absorb consumption amount of grease, reduce further oil yield.
Summary of the invention
In order to solve the problems referred to above, the invention provides the preparation method of a kind of Oleum Vitis viniferae high oil recovery.
The present invention is achieved through the following technical solutions:
The preparation method of a kind of Oleum Vitis viniferae high oil recovery, comprises the steps:
(1) remove impurity: after choosing except large granular impurity, then with sieve, the solid small particles material of Semen Vitis viniferae is screened out, obtain roguing Semen Vitis viniferae standby；
(2) soak: roguing Semen Vitis viniferae is put into immersion 2h in the tartaric acid solution that mass fraction is 3% of its gross mass 4 times, place in the saleratus solution that mass fraction is 3% of its gross mass 4 times immersion 2h after taking-up, be drawn off rinsing well with clear water standby the most again；Semen Vitis viniferae is carried out the cleaning way of alkali after first acid, not only acted as good sterilizing, gone glutinous effect, reduced again seed coat and internal cohesive force, it is simple to follow-up peeling operation；
(3) temperature is boiled: the Semen Vitis viniferae after cleaning is put in the clear water of its gross mass 3 times, and heating keeps water temperature to be 40 ~ 42 DEG C, and temperature is taken out standby after boiling 15 ~ 20min, and temperature is stirred continuously during boiling；
(4) pretreatment: the Semen Vitis viniferae after temperature being boiled is put in hermetic container, carries out cooling process with the cooling rate of 5 ~ 6 DEG C/min to Semen Vitis viniferae, and till Semen Vitis viniferae temperature is down to-6 ~-4 DEG C, period keeps the vacuum in container to be-0.075MPa；
(5) decortication: after being taken out by pretreated Semen Vitis viniferae, carries out stirring decortication with the forced draft of 1.6MPa to it and processes, and keeping ambient temperature during process is 0 ~ 3 DEG C, after 20 ~ 25min；
(6) screening: the Semen Vitis viniferae after decortication being processed takes out and sieves, and after being screened out by seed coat, must remove the peel Semen Vitis viniferae standby；
(7) low-temperature grinding: put in Lowtemperaturepulverizer by peeling Semen Vitis viniferae, controls temperature and is-40 DEG C, it is pulverized to obtain Semen Vitis viniferae powder, and after pulverizing, the size of granule is 0.1 ~ 0.5mm；Low-temperature grinding can not only ensure nutrient substance damage, and the situation causing amount of grease to flow out loss when turn avoid conventional size reduction occurs；
(8) enzymolysis: the Semen Vitis viniferae powder after pulverizing is mixed homogeneously with the clear water of its gross mass 3 times, add the compound enzymic preparation of clear water gross mass 2%, heating keeps water temperature to be 43 ~ 46 DEG C, period is stirred continuously, after enzymolysis 1.5h, described compound enzymic preparation is made up of by weight 7:1:0.5:0.1 cellulase, protease, pectase, tannase；
(9) it is dried: carrying out the enzymolysis solution being mixed into by Semen Vitis viniferae powder and clear water in step (8) heating concentrate drying, heating-up temperature is 65 ~ 70 DEG C, controls the water content of final Semen Vitis viniferae powder less than 5%；
(10) squeezing: dried Semen Vitis viniferae powder is put into and carries out low temperature pressing in squeezer, controlling temperature is 43 ~ 47 DEG C, first squeeze pressure is controlled for 26MPa, after squeezing 20min, then squeeze pressure is promoted to 29MPa, after squeezing 20min, finally squeeze pressure is promoted to 32MPa, squeezing 20min after, more in a manner described circulation primary squeezing after, collect Semen Vitis viniferae crude oil is standby；
(11) purify: carry out getting product after the deacidification of routine, decolouring and deodorize process Oleum Vitis viniferae to Semen Vitis viniferae crude oil.
There is advantages that
(1) present invention has carried out removing the operation of seed coat before Semen Vitis viniferae squeezes, reduce the loss of oils and fats, boiled by first temperature, Cold pretreatment, the technological operation of last high pressure draught decortication again, simple and quick removes Semen Vitis viniferae seed coat, peeling rate is up to more than 95%, and the removal of Semen Vitis viniferae seed coat can save the oil loss of 3 ~ 5%.
(2) before squeezing, Semen Vitis viniferae powder compound enzymic preparation is carried out enzymolysis processing, can further improve the destruction of seed cell wall, it is simple to the leaching of oils and fats, the interpolation of tannase can tannin in enzymolysis Semen Vitis viniferae, reduce its content, it is simple to improve permeability and the mouthfeel of oils and fats.
(3) common cold pressing uses the mode of constant temperature and pressure to carry out, and in certain limit, improves temperature and pressure and can promote oil yield, but temperature crosses conference infringement nutrient substance, and pressure is crossed conference and dramatically increased manufacturing cost, and oil yield promotes inconspicuous；The present invention uses the mode of pressure-cycling to carry out cold pressing extraction, utilizes cytomorphosis under different pressures different, and produces the effect of circulation scaling extrusion, can not only promote oil yield, reduce again the intensity of pressure, save manufacturing cost.
(4) collocation of the inventive method technique is scientific and reasonable, and overall energy consumption is low, and cost is little, does not introduce chemical substance, it is ensured that the safety of Oleum Vitis viniferae, reduces again the content of harmful substance in oils and fats, improves condition and the mouthfeel of oils and fats；The oil yield of final Semen Vitis viniferae can be stablized more than 98%, has good economic benefit and promotional value.
Detailed description of the invention
Embodiment 1
The preparation method of a kind of Oleum Vitis viniferae high oil recovery, comprises the steps:
(1) remove impurity: after choosing except large granular impurity, then with sieve, the solid small particles material of Semen Vitis viniferae is screened out, obtain roguing Semen Vitis viniferae standby；
(2) soak: roguing Semen Vitis viniferae is put into immersion 2h in the tartaric acid solution that mass fraction is 3% of its gross mass 4 times, place in the saleratus solution that mass fraction is 3% of its gross mass 4 times immersion 2h after taking-up, be drawn off rinsing well with clear water standby the most again；
(3) temperature is boiled: the Semen Vitis viniferae after cleaning is put in the clear water of its gross mass 3 times, and heating keeps water temperature to be 40 ~ 42 DEG C, and temperature is taken out standby after boiling 15 ~ 20min, and temperature is stirred continuously during boiling；
(4) pretreatment: the Semen Vitis viniferae after temperature being boiled is put in hermetic container, carries out cooling process with the cooling rate of 5 ~ 6 DEG C/min to Semen Vitis viniferae, and till Semen Vitis viniferae temperature is down to-6 ~-4 DEG C, period keeps the vacuum in container to be-0.075MPa；
(5) decortication: after being taken out by pretreated Semen Vitis viniferae, carries out stirring decortication with the forced draft of 1.6MPa to it and processes, and keeping ambient temperature during process is 0 ~ 3 DEG C, after 20 ~ 25min；
(6) screening: the Semen Vitis viniferae after decortication being processed takes out and sieves, and after being screened out by seed coat, must remove the peel Semen Vitis viniferae standby；
(7) low-temperature grinding: put in Lowtemperaturepulverizer by peeling Semen Vitis viniferae, controls temperature and is-40 DEG C, it is pulverized to obtain Semen Vitis viniferae powder, and after pulverizing, the size of granule is 0.1 ~ 0.5mm；
(8) enzymolysis: the Semen Vitis viniferae powder after pulverizing is mixed homogeneously with the clear water of its gross mass 3 times, add the compound enzymic preparation of clear water gross mass 2%, heating keeps water temperature to be 43 ~ 46 DEG C, period is stirred continuously, after enzymolysis 1.5h, described compound enzymic preparation is made up of by weight 7:1:0.5:0.1 cellulase, protease, pectase, tannase；
(9) it is dried: carrying out the enzymolysis solution being mixed into by Semen Vitis viniferae powder and clear water in step (8) heating concentrate drying, heating-up temperature is 65 ~ 70 DEG C, controls the water content of final Semen Vitis viniferae powder less than 5%；
(10) squeezing: dried Semen Vitis viniferae powder is put into and carries out low temperature pressing in squeezer, controlling temperature is 43 ~ 47 DEG C, first squeeze pressure is controlled for 26MPa, after squeezing 20min, then squeeze pressure is promoted to 29MPa, after squeezing 20min, finally squeeze pressure is promoted to 32MPa, squeezing 20min after, more in a manner described circulation primary squeezing after, collect Semen Vitis viniferae crude oil is standby；
(11) purify: carry out getting product after the deacidification of routine, decolouring and deodorize process Oleum Vitis viniferae to Semen Vitis viniferae crude oil.
Comparative example 1
This comparative example 1 compared with Example 1, does not carries out decortication process to Semen Vitis viniferae, and method step in addition is the most identical.
Matched group
Common glucose seed method for cold pressing.
Above-mentioned three kinds of Semen Vitis viniferae liquefaction modes carrying out result statistics, finds that the oil yield of embodiment 1 reaches 98.5%, the oil yield of comparative example 1 is 95.1%, and the oil yield of matched group is only 91.4%；And the manufacturing cost of embodiment 1 Oleum Vitis viniferae per jin saves 12.4% than matched group.
Claims (1)
1. the preparation method of an Oleum Vitis viniferae high oil recovery, it is characterised in that comprise the steps:
(1) remove impurity: after choosing except large granular impurity, then with sieve, the solid small particles material of Semen Vitis viniferae is screened out, obtain roguing Semen Vitis viniferae standby；
(2) soak: roguing Semen Vitis viniferae is put into immersion 2h in the tartaric acid solution that mass fraction is 3% of its gross mass 4 times, place in the saleratus solution that mass fraction is 3% of its gross mass 4 times immersion 2h after taking-up, be drawn off rinsing well with clear water standby the most again；
(3) temperature is boiled: the Semen Vitis viniferae after cleaning is put in the clear water of its gross mass 3 times, and heating keeps water temperature to be 40 ~ 42 DEG C, and temperature is taken out standby after boiling 15 ~ 20min, and temperature is stirred continuously during boiling；
(4) pretreatment: the Semen Vitis viniferae after temperature being boiled is put in hermetic container, carries out cooling process with the cooling rate of 5 ~ 6 DEG C/min to Semen Vitis viniferae, and till Semen Vitis viniferae temperature is down to-6 ~-4 DEG C, period keeps the vacuum in container to be-0.075MPa；
(5) decortication: after being taken out by pretreated Semen Vitis viniferae, carries out stirring decortication with the forced draft of 1.6MPa to it and processes, and keeping ambient temperature during process is 0 ~ 3 DEG C, after 20 ~ 25min；
(6) screening: the Semen Vitis viniferae after decortication being processed takes out and sieves, and after being screened out by seed coat, must remove the peel Semen Vitis viniferae standby；
(7) low-temperature grinding: put in Lowtemperaturepulverizer by peeling Semen Vitis viniferae, controls temperature and is-40 DEG C, it is pulverized to obtain Semen Vitis viniferae powder, and after pulverizing, the size of granule is 0.1 ~ 0.5mm；
(8) enzymolysis: the Semen Vitis viniferae powder after pulverizing is mixed homogeneously with the clear water of its gross mass 3 times, add the compound enzymic preparation of clear water gross mass 2%, heating keeps water temperature to be 43 ~ 46 DEG C, period is stirred continuously, after enzymolysis 1.5h, described compound enzymic preparation is made up of by weight 7:1:0.5:0.1 cellulase, protease, pectase, tannase；
(9) it is dried: carrying out the enzymolysis solution being mixed into by Semen Vitis viniferae powder and clear water in step (8) heating concentrate drying, heating-up temperature is 65 ~ 70 DEG C, controls the water content of final Semen Vitis viniferae powder less than 5%；
(10) squeezing: dried Semen Vitis viniferae powder is put into and carries out low temperature pressing in squeezer, controlling temperature is 43 ~ 47 DEG C, first squeeze pressure is controlled for 26MPa, after squeezing 20min, then squeeze pressure is promoted to 29MPa, after squeezing 20min, finally squeeze pressure is promoted to 32MPa, squeezing 20min after, more in a manner described circulation primary squeezing after, collect Semen Vitis viniferae crude oil is standby；
(11) purify: carry out getting product after the deacidification of routine, decolouring and deodorize process Oleum Vitis viniferae to Semen Vitis viniferae crude oil.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245370A (en) * | 2017-08-02 | 2017-10-13 | 中方县申太葡萄酒有限公司 | A kind of preparation method of spiny grape seed oil |
| CN113462465A (en) * | 2021-08-13 | 2021-10-01 | 丽江永胜边屯食尚养生园有限公司 | Preparation method of fen-flavor walnut oil |
| RU2787590C1 (en) * | 2022-03-31 | 2023-01-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный технологический университет" (ФГБОУ ВО "КубГТУ") | Method for preparing seeds of nigella sowing (nigella) for oil production |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103232887A (en) * | 2013-04-27 | 2013-08-07 | 辽宁晟麦实业股份有限公司 | Preparation method of pomegranate seed oil |
| CN103351944A (en) * | 2013-04-11 | 2013-10-16 | 石河子市天成油脂有限公司 | Method for extracting grape seed oil by cold pressing |
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2016
- 2016-05-18 CN CN201610328727.1A patent/CN105969510A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103351944A (en) * | 2013-04-11 | 2013-10-16 | 石河子市天成油脂有限公司 | Method for extracting grape seed oil by cold pressing |
| CN103232887A (en) * | 2013-04-27 | 2013-08-07 | 辽宁晟麦实业股份有限公司 | Preparation method of pomegranate seed oil |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107245370A (en) * | 2017-08-02 | 2017-10-13 | 中方县申太葡萄酒有限公司 | A kind of preparation method of spiny grape seed oil |
| CN107245370B (en) * | 2017-08-02 | 2020-12-18 | 中方县申太葡萄酒有限公司 | Preparation method of spiny grape seed oil |
| CN113462465A (en) * | 2021-08-13 | 2021-10-01 | 丽江永胜边屯食尚养生园有限公司 | Preparation method of fen-flavor walnut oil |
| RU2787590C1 (en) * | 2022-03-31 | 2023-01-11 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный технологический университет" (ФГБОУ ВО "КубГТУ") | Method for preparing seeds of nigella sowing (nigella) for oil production |
| RU2788921C1 (en) * | 2022-03-31 | 2023-01-25 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Кубанский государственный технологический университет" (ФГБОУ ВО "КубГТУ") | Method for preparing seeds of nigella sowing (nigella) for oil production |
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Application publication date: 20160928 |