CN105961385A - Novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, seed soaking liquid and germination method - Google Patents
Novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, seed soaking liquid and germination method Download PDFInfo
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- CN105961385A CN105961385A CN201610334788.9A CN201610334788A CN105961385A CN 105961385 A CN105961385 A CN 105961385A CN 201610334788 A CN201610334788 A CN 201610334788A CN 105961385 A CN105961385 A CN 105961385A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/32—Ingredients for reducing the noxious effect of the active substances to organisms other than pests, e.g. toxicity reducing compositions, self-destructing compositions
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- General Health & Medical Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses novel application of tea polyphenol for promoting corn seed germination under heavy metal stress, a seed soaking liquid and a germination method. The seed soaking method comprises the following steps of: (1) sterilizing corn seeds; (2) repeatedly washing the sterilized corn seeds in distilled water; (3) soaking the corn seeds in clean water for 11-12 hours at 26-28 DEG C; (4) watering the corn seeds by using the seed soaking liquid, sealing by using a preservative film, and culturing with sunshine, wherein the seed soaking liquid comprises tea polyphenol; the content of tea polyphenol is 5-80mu g/mL. By virtue of tea polyphenol, the toxicity of chromium ions to seeds can be relieved, a certain promoting effect on corn seeds can be achieved, the activity of the corn seeds can be remarkably improved since seeds treated with tea polyphenol can be germinated in an adverse situation, the toxicity of chromium ions can be inhibited by oxidation of tea polyphenol, the content of chlorophyll can be effectively increased, the activity of CAT can be activated by antioxidation of tea polyphenol, plant cytomembrane peroxidation can be prevented, and the activity of CAT and POD treated with tea polyphenol can be improved.
Description
Technical field
The present invention relates to a kind of tea polyphenols and promote the new application of Course of Corn Seed Germination under heavy metal stress.The present invention
Further relate to the seed-soaking liquid containing tea polyphenols, and use the method that above-mentioned seed-soaking liquid sprouts.
Background technology
Chromium is a kind of element being widely present in nature.Be distributed mainly on rock, soil, big gas and water and
In organism.Chromium distributed pole in soil is wide.Content range is the widest, and in water body and air, chromium content is less.
Trace chromium is then contained in animal and plant body.Nature chromium is mainly presented in bivalent chromium and Cr VI.Two
Sugar in valency chromium participant and animal body and fatty metabolism, be the trace element of needed by human；Cr VI is then
It is clear and definite harmful element, some protein precipitation in blood of human body can be made, cause anemia, nephritis, nerve
The diseases such as inflammation.Long-term contact with Cr VI also can cause respiratory inflammation and induce pulmonary carcinoma or cause invasive
Skin lesion, it is dead that serious Cr VI poisoning also results in people.
Along with industrial expansion, living standards of the people improve constantly, its living environment of mankind's pay attention to day by day.Chromium
Pollution is one of key factor of environmental pollution, at plating, process hides, chemical industry, pigment, metallurgy, fire proofed wood
The industries such as material, the discharge capacity of chromate waste water is continuously increased.In chromate waste water, the existence form of chromium has Gr3++And Gr6+
Two kinds, wherein with Gr6+Toxicity maximum, oneself is verified in many researchs: chromic compound is poisonous, has
The effect that carcinogenic and modificator gene suddenlys change.Cr VI is defined as 17 kinds of height by Environmental Protection Agency USA (EPA)
One of dangerous toxicant.Hexavalent chromium compound oral lethal dose about about 1.5g.Content of 6-valence Cr ions in water
Will be poisoned more than 0.1mg/L.Chromium is similar to arsenic to the toxic action of human body.Its toxicity with valence state, content,
Temperature is different with the person of being applied and changes.Under physiological ph conditions, Cr VI is with CrO42-Form exists penetrates into carefully
Intracellular.CrO4 a few days ago2-Mechanism of carcinogenesis also imperfectly understand.Mainly there are two kinds of viewpoints: one is considered CrO42-
Substantial amounts of free radical (oneself is created during being reduced into pentavalent chromium and tetravalence chromium by intracellular reducing substances
Confirmed by external experiment) substantial amounts of free radical initiation tumor；Another kind is considered Cr VI by intracellular reduction
Material is reduced to bivalent chromium.The bivalent chromium generated rapidly there occurs reaction with DNA (external laboratory observation arrives
The bivalent chromium of reduction and the combination of DNA).Cause the change of genetic code, and then cause sudden change and the cancer of cell
Become.Research finds, chromic long-term absorption can cause the diseases such as squamous cell carcinoma, adenocarcinoma, pulmonary carcinoma；Inhale
Enter the hexavalent chromium compound of high level can cause rhinorrhea, sneeze, scratch where it itches, epistaxis, ulcer and nose
The symptoms such as septal perforation.The contact that short-term is heavy dose of, stimulates and in nose at contact site meeting ulcer, nasal mucosa
Every perforation；Take in over much dosage chromium can cause kidney and the damage of liver and feel sick, gastrointestinal upset,
The symptom such as gastric ulcer, muscle spasm.Circulatory failure can be made time serious, lose consciousness, even dead.
The chromic father and mother of Long Term Contact are also possible to the intelligent development in its lower generation is brought harmful effect.Heavy metal-polluted
In the soil environment of dye, chromium ion coerces lower Course of Corn Seed Germination will be affected by serious.
Summary of the invention
It is an object of the invention to provide a kind of new application of tea polyphenols, specifically tea polyphenols and promote heavy metal stress
The new application of lower Course of Corn Seed Germination.Meanwhile, also provide for the seed-soaking liquid containing tea polyphenols, and use above-mentioned
The method that seed-soaking liquid is sprouted.
In order to reach above-mentioned technical purpose, the technical scheme is that
Tea polyphenols promotes the new application of Course of Corn Seed Germination under heavy metal stress, and corn seed is by heavy metal Cr3+
And Cr6+Before contaminated soil is sprouted, corn seed is soaked in seed-soaking liquid, containing tea polyphenols in seed-soaking liquid,
The content of tea polyphenols is 5～80 μ g/mL.
A kind of promoting the seed-soaking liquid of Course of Corn Seed Germination under heavy metal stress, described seed-soaking liquid contains tea polyphenols,
The content of described tea polyphenols is 5～80 μ g/mL.
A kind of utilize tea polyphenols to promote the method for Course of Corn Seed Germination under heavy metal stress, carry out prior to seeding with
Lower process step:
(1) corn seed is sterilized；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) corn seed is put in clear water, seed soaking 11～12h at 26～28 DEG C；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing tea polyphenols in seed-soaking liquid, the content of tea polyphenols is 5～80 μ g/mL.
In above-mentioned seed-soaking liquid, the content of tea polyphenols is preferably 80 μ g/mL.
Tea polyphenols alleviates the chromium ion murder by poisoning to seed, has certain facilitation to Course of Corn Seed Germination, no
But the murder by poisoning that can effectively suppress chromium ion can also significantly improve germinating energy and germination percentage.Plant after processing with tea polyphenols
Son is sprouted under adverse circumstance and is also made the vigor of corn seed significantly improve, and has laid material base for later growth.
The non-oxidizability of tea polyphenols inhibits the murder by poisoning of chromium ion, is also effectively increased chlorophyllous content.Tea polyphenols
Antioxidation can activate the activity of CAT, to defend plant cell membrane peroxidating.Tea polyphenols process after CAT,
The activity raising of POD.
Soluble protein great majority in plant are the enzymes participating in various metabolism, and surveying its content is to understand
One important indicator of plant total metabolism.Tea polyphenols scavenging capacity oxygen-derived free radicals and directly prevent collagen protein
The attack of receptor 1 activity oxygen and the osmotic potential adjusting in plant has been divided greatly Deng biology.Tea polyphenols improves chromium and coerces down
The content of corn seedling protein.
But, in the case of not having (or when content is the lowest) chromium to coerce, add tea polyphenols and can reduce albumen
Concentration and the activity of antioxidase (SOD, POD and CAT), the sprouting to corn seed causes certain shadow
Ring.In order to solve this problem, seed-soaking liquid adds polydimethylsiloxane and trideceth-6, institute
Stating polydimethyl siloxane content and account for 3～5% (on the basis of seed-soaking liquid quality) of seed-soaking liquid, tridecyl alcohol gathers
Ether-6 content accounts for 1～2% (on the basis of seed-soaking liquid quality) of seed-soaking liquid.Trideceth-6 can suitably change
Become pH value, the polydimethylsiloxane characteristic such as then utilize it hydrophobic, in the case of not having chromium to coerce, eliminate
The impact of tea polyphenols, will not reduce protein concentration and the activity of antioxidase (SOD, POD and CAT).
Accompanying drawing explanation
Fig. 1 is the tea polyphenols impact on corn protein concentration.
Fig. 2 is the tea polyphenols impact on maize peroxidase vigor.
Fig. 3 is the tea polyphenols impact on Semen Maydis activity of catalase.
Detailed description of the invention
The present invention is further detailed explanation with detailed description of the invention below in conjunction with the accompanying drawings.
Corn seed is sweet No. 1 of Qin Nong.Tea polyphenols is provided by Wuxi Taiyo Green Power Co., Ltd., pure
Degree > 99%.Polydimethyl siloxane content and trideceth-6 are by Shanghai Mike's limited public affairs of woods biochemical technology
Department provides.
Embodiment 1
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 12h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Seed-soaking liquid contains the K of 40mg/L2Cr2O7。
Embodiment 2
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 11h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing tea polyphenols and the K of 40mg/L that content is 5 μ g/mL in seed-soaking liquid2Cr2O7。
Embodiment 3
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 12h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing tea polyphenols and the K of 40mg/L that content is 20 μ g/mL in seed-soaking liquid2Cr2O7。
Embodiment 4
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 11h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing tea polyphenols and the K of 40mg/L that content is 80 μ g/mL in seed-soaking liquid2Cr2O7。
Embodiment 5
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 12h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing tea polyphenols and the K of 40mg/L that content is 320 μ g/mL in seed-soaking liquid2Cr2O7。
Embodiment 6
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 12h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing the tea polyphenols that content is 80 μ g/mL in seed-soaking liquid.
Embodiment 7
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 11h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing content in seed-soaking liquid is the tea polyphenols of 80 μ g/mL, 5% (on the basis of seed-soaking liquid quality) poly-two
Methylsiloxane content and 1% (on the basis of seed-soaking liquid quality) trideceth-6.
Embodiment 8
(1) corn seed is put into immersion half an hour in the potassium permanganate solution that concentration is 1%, and ceaselessly stirs
Mix, thus carry out sterilization for seed；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) putting in clear water by corn seed, soak seed at 26～28 DEG C 12h；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and train in the sun
Support；Containing content in seed-soaking liquid is the tea polyphenols of 20 μ g/mL, 5% (on the basis of seed-soaking liquid quality) poly-two
Methylsiloxane content and 2% (on the basis of seed-soaking liquid quality) trideceth-6.
After above-described embodiment corn germinal, tea polyphenols is to corn protein concentration, peroxidase activity and peroxide
Change the impact of hydrogen enzyme activity as Figure 1-3.Dotted line represents in the case of Chrome-free coerces, and contains in seed-soaking liquid
Impact when tea polyphenols, polydimethylsiloxane and trideceth-6.Zone circle lines represent that Chrome-free is coerced
In the case of, seed-soaking liquid comprises only impact during tea polyphenols.
As seen from Figure 1, the tea polyphenols of 5～80 μ g/mL significantly improves chromium after processing and coerces lower corn seedling
The content of protein.In the case of Chrome-free is coerced, when seed-soaking liquid comprises only tea polyphenols, affect corn seedling
The content of protein.In the case of Chrome-free is coerced, the polydimethylsiloxane contained in seed-soaking liquid and tridecane
Alcohol polyethers-6 eliminates the tea polyphenols impact on corn seedling protein content.
As seen from Figure 2, the tea polyphenols of 5～80 μ g/mL significantly improves chromium after processing and coerces lower corn seedling
Peroxidase activity.In the case of Chrome-free is coerced, when seed-soaking liquid comprises only tea polyphenols, affect Semen Maydis children
Seedling peroxidase activity.In the case of Chrome-free is coerced, the polydimethylsiloxane contained in seed-soaking liquid and ten
Three alkanol polyethers-6 eliminate the tea polyphenols impact on corn seedling peroxidase activity.
As seen from Figure 3, the tea polyphenols of 5～80 μ g/mL significantly improves chromium after processing and coerces lower corn seedling
Activity of catalase.In the case of Chrome-free is coerced, when seed-soaking liquid comprises only tea polyphenols, affect Semen Maydis children
Seedling activity of catalase.In the case of Chrome-free is coerced, the polydimethylsiloxane contained in seed-soaking liquid and ten
Three alkanol polyethers-6 eliminate the tea polyphenols impact on corn seedling activity of catalase.
Above-described embodiment limits the present invention, every employing equivalent or the side of equivalent transformation never in any form
The technical scheme that formula obtains all falls within protection scope of the present invention.
Claims (9)
1. the new application of Course of Corn Seed Germination under tea polyphenols promotes heavy metal stress, it is characterised in that: corn seed is by heavy metal Cr3+And Cr6+Before sprouting in contaminated soil, being soaked by corn seed in seed-soaking liquid, containing tea polyphenols in seed-soaking liquid, the content of tea polyphenols is 5～80 μ g/mL.
Tea polyphenols the most according to claim 1 promotes the new application of Course of Corn Seed Germination under heavy metal stress, it is characterised in that: in described seed-soaking liquid, the content of tea polyphenols is 80 μ g/mL.
Tea polyphenols the most according to claim 1 and 2 promotes the new application of Course of Corn Seed Germination under heavy metal stress, it is characterized in that: containing polydimethylsiloxane and trideceth-6 in described seed-soaking liquid, described polydimethyl siloxane content accounts for the 3～5% of seed-soaking liquid, and trideceth-6 content accounts for the 1～2% of seed-soaking liquid.
4. one kind promotes the seed-soaking liquid of Course of Corn Seed Germination under heavy metal stress, it is characterised in that: described seed-soaking liquid contains tea polyphenols, and the content of described tea polyphenols is 5～80 μ g/mL.
The most according to claim 4 a kind of promote the seed-soaking liquid of Course of Corn Seed Germination under heavy metal stress, it is characterised in that: in described seed-soaking liquid, the content of tea polyphenols is 80 μ g/mL.
6. promote the seed-soaking liquid of Course of Corn Seed Germination under heavy metal stress according to a kind of described in claim 4 or 5, it is characterized in that: containing polydimethylsiloxane and trideceth-6 in described seed-soaking liquid, described polydimethyl siloxane content accounts for the 3～5% of seed-soaking liquid, and trideceth-6 content accounts for the 1～2% of seed-soaking liquid.
7. one kind utilizes tea polyphenols to promote the method for Course of Corn Seed Germination under heavy metal stress, it is characterised in that carry out following process step prior to seeding:
(1) corn seed is sterilized；
(2) corn seed after sterilization is rinsed well in distilled water repeatedly；
(3) corn seed is put in clear water, seed soaking 11～12h at 26～28 DEG C；
(4) with seed-soaking liquid, corn seed is watered, then seal with preservative film, put and cultivate in the sun；Containing tea polyphenols in seed-soaking liquid, the content of tea polyphenols is 5～80 μ g/mL.Continuing 5 days, every day waters.
The most according to claim 7 a kind of tea polyphenols is utilized to promote the method for Course of Corn Seed Germination under heavy metal stress, it is characterised in that: in described seed-soaking liquid, the content of tea polyphenols is 80 μ g/mL.
9. utilize tea polyphenols to promote the method for Course of Corn Seed Germination under heavy metal stress according to a kind of described in claim 7 or 8, it is characterized in that: containing polydimethylsiloxane and trideceth-6 in described seed-soaking liquid, described polydimethyl siloxane content accounts for the 3～5% of seed-soaking liquid, and trideceth-6 content accounts for the 1～2% of seed-soaking liquid.
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| CN110976503A (en) * | 2019-12-16 | 2020-04-10 | 湖南大学 | Modified attapulgite and preparation method and application thereof |
| CN111670768A (en) * | 2020-07-01 | 2020-09-18 | 榆林学院 | Loess arid region corn dry farming water-saving cultivation method |
| CN113924840A (en) * | 2021-10-11 | 2022-01-14 | 温州科技职业学院 | Seed soaking and germination accelerating method capable of improving performance of rice seeds |
| CN114793528A (en) * | 2022-03-25 | 2022-07-29 | 浙江大学 | Application of nano enzyme in promoting corn growth under heavy metal stress |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110976503A (en) * | 2019-12-16 | 2020-04-10 | 湖南大学 | Modified attapulgite and preparation method and application thereof |
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| CN113924840A (en) * | 2021-10-11 | 2022-01-14 | 温州科技职业学院 | Seed soaking and germination accelerating method capable of improving performance of rice seeds |
| CN114793528A (en) * | 2022-03-25 | 2022-07-29 | 浙江大学 | Application of nano enzyme in promoting corn growth under heavy metal stress |
| CN114793528B (en) * | 2022-03-25 | 2023-02-14 | 浙江大学 | Application of nano enzyme in promoting corn growth under heavy metal stress |
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| CN105961385B (en) | 2018-11-30 |
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