CN105950498B - The production method and its biological denitrification method of Paracoccus denitrificans - Google Patents
The production method and its biological denitrification method of Paracoccus denitrificans Download PDFInfo
- Publication number
- CN105950498B CN105950498B CN201610282573.7A CN201610282573A CN105950498B CN 105950498 B CN105950498 B CN 105950498B CN 201610282573 A CN201610282573 A CN 201610282573A CN 105950498 B CN105950498 B CN 105950498B
- Authority
- CN
- China
- Prior art keywords
- paracoccus denitrificans
- waste water
- sweet potato
- potato starch
- processing waste
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of production method of Paracoccus denitrificans and its biological denitrification methods, pH to 5.0~7.0 including adjusting Sweet Potato Starch Processing Waste Water with sodium hydroxide or hydrochloric acid, it sterilizes 10~35 minutes under the conditions of 110~126 DEG C, as Sweet Potato Starch Processing Waste Water culture medium；The Paracoccus denitrificans bacterial strain saved to Sweet Potato Starch Processing Waste Water inoculation of medium inclined-plane, then in 25~30 DEG C, shaken cultivation 20~30 hours, obtain liquid seeds under the conditions of 150~200rpm；To the liquid seeds of Sweet Potato Starch Processing Waste Water inoculation of medium 2~10%, then shaken cultivation 18~30 hours under the conditions of 25~30 DEG C.The present invention selects fermentation substrate of the cheap sweet potato waste water as Paracoccus denitrificans, to reduce the pollution control investment of enterprise, while can reduce the production cost of Paracoccus denitrificans again, to realize that waste resource recovery utilizes.
Description
Technical field
The present invention relates to waste resource recovery and technical field of water environment treatment, and in particular to a kind of production of Paracoccus denitrificans
Method and its biological denitrification method.
Background technique
Composite filling is developed on the basis of soft-filler and semi soft packing, and structure is by plastic disks pressure
Button is changed to the big plastic hoop of double-round, and hydroformylation fiber or dacron thread are pressed on the ring of ring, fibre bundle is uniformly distributed；Inner ring is snow
Flower-shaped plastics branch biofilm and can effectively cut bubble, improve the transfer rate and utilization rate of oxygen.Make aqueous vapor biomembrane
It is sufficiently exchanged, the organic matter in water is made to obtain efficient process.It is currently used primarily in sewage, waste water treatment engineering, is matched in
The equipment such as contact oxidation tower, oxidation pond oxidation trough are the biologies of a kind of biological contact oxidation process and Unareobic fermentation processing waste water
Carrier.However composite filling self is colonized and is bred by microorganism in the great water bodies environment such as contaminated river, lake
Have the shortcomings that the biofilm formation time is long, contaminant removal efficiency is low, how to improve composite filling and polluted in water body environment
The removal efficiency of the degradation capability of object especially ammonia nitrogen and nitrate nitrogen becomes the technology of black and odorous water elimination and eutrophication control
Difficult point, then the biological denitrification process of ammonia nitrogen and nitrate nitrogen becomes the core cell for improving composite filling performance.
Traditional biological denitrogenation is divided into two processes of aerobic nitrification and anaerobic denitrifying, due to reaction condition difference, need by
Nitrification and denitrification process separates, so that treatment process generates many drawbacks.Nineteen eighty-three, Robertson and Kuenen are sieved for the first time
Selecting one plant had not only had heterotrophic nitrification simultaneously but also had had bacterium --- the general feeding sulphur coccus (Thiosphaera of biological aerobic denitrification ability
Pantotropha), it was demonstrated that the presence of heterotrophic nitrification-biological aerobic denitrification phenomenon, this plant of bacterium are now renamed as Paracoccus denitrificans
(Paracoccus denitrificans).Paracoccus denitrificans is a kind of Gram-negative bacteria of the inorganic autotrophy of facultativeization energy, it
Heterotrophic growth can be carried out using organic matters such as various carbohydrates, amino acid, it also can be in H2、O2、CO2In the case where existing simultaneously
Carry out autophyting growth.
Utilize the investment that should actually need a large amount of thallus in the process of Paracoccus denitrificans purifying water body nitrogen, at present denitrogenation
Referring to the mode of standard biologic fermentation, fermentation substrate is mainly bacterium class culture medium, fermentation medium group for the production of secondary coccus
At mainly having: 20 grams of glucose, 8 grams of beef extract, 5 grams of L-sodium, MgSO40.5 gram, 0.5 gram of KCl, KH2PO41 gram,
FeSO4·6H20.15 milligram of O, MnSO4·H25 milligrams of O, CuSO4·5H20.16 milligram of O, 1000 milliliters of (Chinese inventions of water
Patent (application number: discloses the raw materials for production of bacillus amyloliquefaciens) in 201010264520.5).According to market purchasing price
Calculating cost of material is about 3500 yuan/ton, caused since fermentation costs are high cost using Paracoccus denitrificans purifying water body compared with
Height limits its extensive use.
Summary of the invention
In view of this, it is an object of the invention to propose a kind of production method of Paracoccus denitrificans and its biological denitrificaion side
Method to reduce the production cost of Paracoccus denitrificans, while improving its Nitrogen removal effect.
Based on above-mentioned purpose, the present invention provides a kind of production methods of Paracoccus denitrificans, comprising the following steps:
The pH to 5.0~7.0 that Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, goes out under the conditions of 110~126 DEG C
Bacterium 10~35 minutes, as Sweet Potato Starch Processing Waste Water culture medium；
The Paracoccus denitrificans bacterial strain saved to Sweet Potato Starch Processing Waste Water inoculation of medium inclined-plane, then in 25~30 DEG C, 150
Shaken cultivation 20~30 hours, obtain liquid seeds under the conditions of~200rpm；
To the liquid seeds of Sweet Potato Starch Processing Waste Water inoculation of medium 2~10%, then vibrated under the conditions of 25~30 DEG C
Culture 18~30 hours.
In some embodiments of the invention, the solution obtained after cultivation through count plate, viable count up to 1.1 ×
109CFU/ml or more.
The present invention also provides a kind of biological denitrificaions of liquid bacterial agent that the production method according to above-mentioned Paracoccus denitrificans obtains
Method, comprising:
Plastic disks clinching is changed to the big plastic hoop of double-round, hydroformylation fiber or dacron thread are pressed on the ring of ring, fibre is made
Dimension beam is uniformly distributed；
The liquid bacterial agent is packed into spray thrower tank, spray thrower is opened in 100,000 grades of clean workrooms and sprays liquid bacterial agent
Onto the soft microbe stuffing, it is sprayed onto the adsorbance saturation of filler；It is sent into vacuum drying cabinet by closure delivering device, is done
35~40 DEG C of dry room temperature, it is 8~10 hours dry, that is, Paracoccus denitrificans biologic packing material is made；
Paracoccus denitrificans biologic packing material is serially connected on fixed strip, assembled fixed strip is directly installed on static water body ring
In border.
In some embodiments of the invention, the inner ring of the big plastic hoop of the double-round is flakes plastics branch, and selection is poly-
Propylene bulked silk is raw material, the soft microbe stuffing being woven into, the fixation support as Paracoccus denitrificans fermentation liquid.
In some embodiments of the invention, the fixed strip is on rigid nylon rope or leather strap, every meter of fixed strip
Concatenate 6~8 Paracoccus denitrificans fillers.
From the above it can be seen that the present invention, which provides Paracoccus denitrificans and its biological denitrification method, reduces denitrogenation pair ball
The production cost of bacterium, the present invention selects fermentation substrate of the cheap sweet potato waste water as Paracoccus denitrificans, to reduce enterprise
Pollution control investment, while the production cost of Paracoccus denitrificans can be reduced again, to realize that waste resource recovery utilizes；Further,
Denitrifier of the present invention and composite filling organically combine the microbiocidal composite filling to be formed, and Paracoccus denitrificans is utilized mechanical spray
Mode be largely fixed on composite filling, realize largely colonizing and prepare microbiocidal composite filling for Paracoccus denitrificans；Most
The microbiocidal composite filling is applied in the biological denitrification process of polluted water body afterwards, realizes the fast prompt drop to pollutant
Solution.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, to this hair
Bright further description.
Sweet potato waste water is the by-product of sweet potato processing, and enterprise generally makees anaerobic-aerobic treatment as pollutant, is polluted
Processing cost is about 10~15 yuan/ton.For the production cost for reducing Paracoccus denitrificans, the present invention selects cheap sweet potato waste water to make
For the fermentation substrate of Paracoccus denitrificans, to reduce the pollution control investment of enterprise, while the life of Paracoccus denitrificans can be reduced again
Cost is produced, to realize that waste resource recovery utilizes.
Embodiment 1
The production method of Paracoccus denitrificans provided by the invention the following steps are included:
1) Sweet Potato Starch Processing Waste Water pre-processes: the pH to 6.0 of Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, then at
It sterilizes 30 minutes under the conditions of 110~115 DEG C.
2) prepared by liquid seeds: the denitrogenation pair that inoculation inclined-plane saves into the triangular flask equipped with Sweet Potato Starch Processing Waste Water culture medium
Meningitidis strains (pd1222), then in 30 DEG C, shaken cultivation for 24 hours, obtains liquid seeds under the conditions of 180rpm.
3) expand culture: to the liquid seeds equipped with the Sweet Potato Starch Processing Waste Water inoculation of medium 8% pre-processed, then
Constant temperature oscillation shaking table culture 28 hours under the conditions of 25~27 DEG C；Solution after culture is through count plate, viable count
Up to 1.1 × 109CFU/ml or more.The solution obtained after expanding culture can be used as liquid bacterial agent and directly use.
Embodiment 2
The production method of Paracoccus denitrificans provided by the invention the following steps are included:
1) Sweet Potato Starch Processing Waste Water pre-processes: the pH to 6.2 of Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, then at
It sterilizes 28 minutes under the conditions of 120~125 DEG C.
2) prepared by liquid seeds: the denitrogenation pair that inoculation inclined-plane saves into the triangular flask equipped with Sweet Potato Starch Processing Waste Water culture medium
Meningitidis strains (pd1222), then in 28 DEG C, shaken cultivation 27h under the conditions of 160rpm obtains liquid seeds.
3) expand culture: to the liquid seeds equipped with the Sweet Potato Starch Processing Waste Water inoculation of medium 5% pre-processed, then
Constant temperature oscillation shaking table culture 22 hours under the conditions of 29 DEG C；Solution after culture is through count plate, and viable count is up to 1.2
×109CFU/ml or more.The solution obtained after expanding culture can be used as liquid bacterial agent and directly use.
Embodiment 3
The production method of Paracoccus denitrificans provided by the invention the following steps are included:
1) Sweet Potato Starch Processing Waste Water pre-processes: the pH to 7.0 of Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, then at
It sterilizes 15 minutes under the conditions of 118~122 DEG C.
2) prepared by liquid seeds: the denitrogenation pair that inoculation inclined-plane saves into the triangular flask equipped with Sweet Potato Starch Processing Waste Water culture medium
Meningitidis strains (pd1222), then in 25 DEG C, shaken cultivation 22h under the conditions of 150rpm obtains liquid seeds.
3) expand culture: to the liquid seeds equipped with the Sweet Potato Starch Processing Waste Water inoculation of medium 3% pre-processed, then
Constant temperature oscillation shaking table culture 20 hours under the conditions of 29~30 DEG C；Solution after culture is through count plate, viable count
Up to 1.3 × 109CFU/ml or more.The solution obtained after expanding culture can be used as liquid bacterial agent and directly use.
Embodiment 4
The production method of Paracoccus denitrificans provided by the invention the following steps are included:
1) Sweet Potato Starch Processing Waste Water pre-processes: the pH to 5.5 of Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, then at
It sterilizes 10 minutes under the conditions of 113~126 DEG C.
2) prepared by liquid seeds: the denitrogenation pair that inoculation inclined-plane saves into the triangular flask equipped with Sweet Potato Starch Processing Waste Water culture medium
Meningitidis strains (pd1222), then in 25 DEG C, shaken cultivation 23h under the conditions of 195rpm obtains liquid seeds.
3) expand culture: to the liquid seeds equipped with the Sweet Potato Starch Processing Waste Water inoculation of medium 2% pre-processed, then
Constant temperature oscillation shaking table culture 24 hours under the conditions of 28~29 DEG C；Solution after culture is through count plate, viable count
Up to 1.2 × 109CFU/ml or more.The solution obtained after expanding culture can be used as liquid bacterial agent and directly use.
By the way that as can be seen that the present invention is using Sweet Potato Starch Processing Waste Water as culture medium, cost of material can save 3500 above
Yuan/ton, so that the production cost of Paracoccus denitrificans be made to substantially reduce, while 10~15 yuan/ton of processing cost of pollution can also be saved.
Further, the liquid bacterial agent obtained by aforementioned production method is used for biological denitrificaion by the present invention, and the biology is de-
Nitrogen method includes:
Liquid bacterial agent is fixed on composite filling in the way of machinery spray, realizes a large amount of fixed of Paracoccus denitrificans
It grows, prepares microbiocidal composite filling；
The microbiocidal composite filling is applied in the biological denitrification process of polluted water body, is realized to the fast of pollutant
Prompt drop solution.
Specifically, microbiocidal composite filling combined method may comprise steps of:
1) Paracoccus denitrificans carrier filler is prepared:
Firstly, plastic disks clinching is changed to the big plastic hoop of double-round, hydroformylation fiber or dacron thread are pressed on the ring of ring,
It is uniformly distributed fibre bundle；Inner ring is flakes plastics branch, and the polypropylene bulked silk that can choose large specific surface area is raw material,
The soft microbe stuffing being woven into, as the fixation support of liquid bacterial agent, the specific surface of the polypropylene bulked silk
Product can be 600~800m2/m3。
2) Paracoccus denitrificans biologic packing material is prepared:
The liquid bacterial agent is packed into spray thrower tank, spray thrower is opened in 100,000 grades of clean workrooms and sprays liquid bacterial agent
Onto the soft microbe stuffing, it is sprayed onto the adsorbance saturation of filler (lower end, which is dripped, to be advisable).It is sent by closure delivering device
Enter vacuum drying cabinet, dry 35~40 DEG C of room temperature is 8~10 hours dry, that is, Paracoccus denitrificans biologic packing material is made.
3) fixation and assembling of biologic packing material
Paracoccus denitrificans biologic packing material is serially connected on fixed strip, the fixed strip can be selected from rigid nylon rope or skin
Item, 6~8 Paracoccus denitrificans fillers are concatenated on every meter of fixed strip, and assembled fixed strip can be directly mounted at static water body ring
In border, also the mountable bottom on chinampa, plays the effect that microorganism biological is strengthened.
Embodiment 5: biological denitrificaion simulated experiment of the microbiocidal composite filling to nitric wastewater
It prepares and simulates low nitrogen sewage I:
BOD concentration is 0mg/L, NH4+- N concentration 10mg/L, NO3 -- N concentration 10mg/L.Control group: conventional combination filler
Dispensing ratio is the composite filling that every cube of water body launches 0.05-0.1 cubes, in 30 DEG C of temperature micro- oxygen (DO=2mg/L) item
96h is handled under part.NH4+With NO3 -It is 34%, 21% that removal efficiency is reachable respectively；Experimental group: the throwing of microbiocidal composite filling
Putting ratio is the microbiocidal composite filling that every cube of water body launches 0.05-0.1 cubes, in 30 DEG C of temperature of micro- oxygen (DO=
96h is handled under the conditions of 2mg/L).NH4+With NO3 -It is 88%, 61% that removal efficiency is reachable respectively.；
Simulate high nitrogen sewage II:
BOD concentration is 0mg/L, NH4+- N concentration 20mg/L, NO3-- N concentration 20mg/L；Control group: conventional combination filler
Dispensing ratio is the composite filling that every cube of water body launches 0.05-0.1 cubes, in 30 DEG C of temperature micro- oxygen (DO=2mg/L) item
96h is handled under part.NH4+With NO3 -It is 46%, 27% that removal efficiency is reachable respectively,.The dispensing ratio of microbiocidal composite filling
The composite filling that 0.05-0.1 cubes is launched for every cube of water body, is handled under the conditions of 30 DEG C of temperature of micro- oxygen (DO=2mg/L)
96h。NH4+With NO3 -It is 87%, 76% that removal efficiency is reachable respectively.
In conclusion the present invention selects cheap sweet potato waste water to make present invention reduces the production cost of Paracoccus denitrificans
For the fermentation substrate of Paracoccus denitrificans, to reduce the pollution control investment of enterprise, while the life of Paracoccus denitrificans can be reduced again
Cost is produced, to realize that waste resource recovery utilizes.Further, denitrifier of the present invention and composite filling organically combine the micro- life to be formed
Paracoccus denitrificans is largely fixed on composite filling in the way of machinery spray, realizes denitrogenation pair ball by object type composite filling
Microbiocidal composite filling is prepared in largely colonizing for bacterium；The microbiocidal composite filling is finally applied to polluted water body
In biological denitrification process, the fast degradation to pollutant is realized.
It should be understood by those ordinary skilled in the art that: the discussion of any of the above embodiment is exemplary only, not
It is intended to imply that the scope of the present disclosure (including claim) is limited to these examples；Under thinking of the invention, above embodiments
Or can also be combined between the technical characteristic in different embodiments, step can be realized with random order, and be existed such as
Many other variations of the upper different aspect of the invention, for simplicity, they are not provided in details.Therefore, it is all
Within the spirit and principles in the present invention, any omission, modification, equivalent replacement, improvement for being made etc. be should be included in of the invention
Within protection scope.
Claims (3)
1. a kind of biological denitrification method of Paracoccus denitrificans, which comprises the following steps:
The pH to 5.0~7.0 that Sweet Potato Starch Processing Waste Water is adjusted with sodium hydroxide or hydrochloric acid, sterilizes 10 under the conditions of 110~126 DEG C
~35 minutes, as Sweet Potato Starch Processing Waste Water culture medium；
The Paracoccus denitrificans bacterial strain saved to Sweet Potato Starch Processing Waste Water inoculation of medium inclined-plane, then in 25~30 DEG C, 150~
Shaken cultivation 20~30 hours, obtain liquid seeds under the conditions of 200rpm；
To the liquid seeds of Sweet Potato Starch Processing Waste Water inoculation of medium 2~10%, the then shaken cultivation under the conditions of 25~30 DEG C
Obtain liquid bacterial agent within 18~30 hours；
Plastic disks clinching is changed to the big plastic hoop of double-round, hydroformylation fiber or dacron thread are pressed on the ring of ring, fibre bundle is made
It is uniformly distributed, the inner ring of the big plastic hoop of double-round is flakes plastics branch, selects polypropylene bulked silk for raw material, is compiled
The soft microbe stuffing being made into, the fixation support as Paracoccus denitrificans fermentation liquid；
The liquid bacterial agent is packed into spray thrower tank, spray thrower is opened in 100,000 grades of clean workrooms by liquid bacterial agent and is sprayed onto institute
It states on soft microbe stuffing, is sprayed onto the adsorbance saturation of filler；Vacuum drying cabinet, hothouse are sent by closure delivering device
It is 35~40 DEG C of temperature, 8~10 hours dry, that is, Paracoccus denitrificans biologic packing material is made；
Paracoccus denitrificans biologic packing material is serially connected on fixed strip, assembled fixed strip is directly installed on static water body environment
In.
2. biological denitrification method according to claim 1, which is characterized in that the solution obtained after cultivation is through plate bacterium
It counts, viable count is up to 1.1 × 109CFU/ml or more.
3. biological denitrification method according to claim 1, which is characterized in that the fixed strip be selected from rigid nylon rope or
Leather strap concatenates 6~8 Paracoccus denitrificans fillers on every meter of fixed strip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610282573.7A CN105950498B (en) | 2016-04-29 | 2016-04-29 | The production method and its biological denitrification method of Paracoccus denitrificans |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610282573.7A CN105950498B (en) | 2016-04-29 | 2016-04-29 | The production method and its biological denitrification method of Paracoccus denitrificans |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105950498A CN105950498A (en) | 2016-09-21 |
| CN105950498B true CN105950498B (en) | 2019-10-18 |
Family
ID=56914571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610282573.7A Active CN105950498B (en) | 2016-04-29 | 2016-04-29 | The production method and its biological denitrification method of Paracoccus denitrificans |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105950498B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107034152A (en) * | 2016-10-11 | 2017-08-11 | 天津科技大学 | A kind of method that use sweet potato starch waste liquid prepares azotate pollution soil remediation microbial inoculum N4 |
| CN116814483B (en) * | 2023-06-15 | 2024-07-16 | 黄河三角洲京博化工研究院有限公司 | Efficient anoxic denitrification strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN104671416A (en) * | 2015-02-15 | 2015-06-03 | 中国科学院生态环境研究中心 | Device for restoring eutrophic water |
-
2016
- 2016-04-29 CN CN201610282573.7A patent/CN105950498B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071141A (en) * | 2010-03-26 | 2011-05-25 | 无锡中佳科技股份有限公司 | Denitrified microbial agent as well as preparation method and application thereof |
| CN104671416A (en) * | 2015-02-15 | 2015-06-03 | 中国科学院生态环境研究中心 | Device for restoring eutrophic water |
Non-Patent Citations (1)
| Title |
|---|
| An improved medium for the anaerobic growth of Paracoccus denitrificans Pd1222.;Hahnke SM;《Front Microbiol.》;20140131;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105950498A (en) | 2016-09-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Pokorna et al. | Sulfur-oxidizing bacteria in environmental technology | |
| CN105368745B (en) | Composite microbial preparation for treating black and odorous rivers and preparation method thereof | |
| CN104673738B (en) | A kind of domestication of heterotrophic nitrification aerobic denitrifying bacteria and screening technique | |
| CN103013872B (en) | Heterotrophic nitrifying and aerobic denitrifying bacterium and culture and application thereof | |
| JP5414090B2 (en) | Solid support on which microorganism group performing parallel dual mineralization reaction is immobilized, catalyst column, and method for producing solid medium for plant cultivation | |
| CN103122332B (en) | Achromobacter xylosoxidans subsp.xylosoxidans LH-N25 and heterotrophic nitrification and aerobic denitrification microorganism bactericide and preparation method and use thereof | |
| CN101691547B (en) | Method for restoring micro-scale polluted reservoir water by using situ bio-contact oxidation | |
| CN102533623B (en) | Achromobacter xylosoxidans with denitrification and dephosphorization function and application of Achromobacter xylosoxidans | |
| CN106987547A (en) | One plant of Acinetobacter bauamnnii and its application | |
| CN106635923A (en) | Method for preparing high-density salt-tolerant denitrifying bacterium agent applicable to wastewater treatment | |
| CN105950498B (en) | The production method and its biological denitrification method of Paracoccus denitrificans | |
| CN106145375A (en) | A kind of preparation and application method for removing sewage and the compositions educating river course again | |
| CN112358043A (en) | Efficient deep denitrification filler for water treatment | |
| CN101817589B (en) | Microbial ecological bed and method for treating inland river by using microbial ecological bed | |
| CN107630012A (en) | A kind of embedding method of aerobic denitrifying bacteria | |
| CN205088059U (en) | Rural low contaminated water deep purification system | |
| CN101434905A (en) | Method for screening nitrification bacteria | |
| CN104232543A (en) | Method for removing nitrate nitrogen and total phosphorus in printing and dyeing wastewater by denitrifying poly-phosphorus accumulating organisms (B8) | |
| CN101724583B (en) | Method for producing microorganisms for processing ammonia-containing wastewater | |
| Rani et al. | Prospecting the significance of methane-utilizing bacteria in agriculture | |
| CN105036482A (en) | Countryside low-pollution water deep purification system | |
| KR101061690B1 (en) | Deodorization system capable of supplying liquid fertilizer production strain and liquid fertilizer production facility comprising the deodorization system | |
| CN110140473A (en) | A kind of method of efficient water purification plant culture growth-promoting | |
| CN104671416A (en) | Device for restoring eutrophic water | |
| CN101348300A (en) | Method for removing aquaculture water nitrite nitrogen by aerobic denitrification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |