CN105943743A - Callicarpa nudiflora and pollen typhae liquid for treating burns and scalds and preparation method thereof - Google Patents
Callicarpa nudiflora and pollen typhae liquid for treating burns and scalds and preparation method thereof Download PDFInfo
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- A61K36/88—Liliopsida (monocotyledons)
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Abstract
The invention belongs to the field of traditional Chinese medicine production and relates to a callicarpa nudiflora and pollen typhae liquid for treating burns and scalds. The liquid is characterized by mixing callicarpa nudiflora with pollen typhae and extracting the mixture with water and concentrating an extracting solution until the extracting solution per ml contains 0.08-0.12g of callicarpa nudiflora crude medicine, thus obtaining a callicarpa nudiflora and pollen typhae water extract; and mixing the callicarpa nudiflora and pollen typhae water extract with a chitosan solution, then carrying out a water bath method and filtration, collecting a clear liquid and carrying out subpackage and sterilization, thus obtaining the callicarpa nudiflora and pollen typhae liquid. The callicarpa nudiflora and pollen typhae liquid has the beneficial effects that a preparation process is simple; callicarpa nudiflora and pollen typhae are used as raw materials to undergo scientific compatibility and synergism; heating reflux extraction is adopted; the water extract contains many varieties of active ingredients; the content of total active ingredients is high; and the callicarpa nudiflora and pollen typhae liquid has the obvious function of treating burns and scalds through external use, has the characteristics of low cost, high content of active substances, good treatment effects on burns and scalds, and the like, and has great clinical application potential.
Description
Technical field
The invention belongs to field of traditional Chinese medicine pharmacy, relate to a kind of Chinese medicine preparation treating burn and scald, specifically one is controlled
Folium Callicarpae Formosanae Pollen Typhae treatment liquid treating burn and scald and preparation method thereof.
Background technology
Burn and scald, ancient medical book puts a chimney on the stove and make the fire draw wound etc. also known as fire burn, burn, burn, soup, and general name extreme misery scalds
Wound, China's Chinese Traditional Medicine have accumulated rich experiences in terms for the treatment of burn and scald.
At present, the treatment burn and scald medicine of Clinical practice is divided into Chinese medicine preparation, Western medicine preparation and Chinese and western medicine knot
Close therapy.The form of Chinese drug being usually used in treatment burn has: oil preparation, such as Oleum Radix Arnebiae (Oleum Radix Lithospermi), Adeps melis etc.；Unguentum is as burnt
Vulnerary cream, Scald Ointment etc.；Powder: such as boiling hot gunpowder, single Rhizoma Polygoni Cuspidati powder etc.；Membrane: such as burn medicine film etc.；
Aqueous solvent: such as Kangfuxin Drop, compound recipe bear bile fluid etc.；Spray: such as burn, SHAOSHANG PENWUJI etc.；
Also have cream, film former, such as compound Chinese aloe cream, " white magic flower " liniment etc..From documents and materials report point
Analysis, in burn treating, Borneolum Syntheticum, Radix Sanguisorbae, Radix Et Rhizoma Rhei, Rhizoma Coptidis, Cortex Phellodendri, Rhizoma Polygoni Cuspidati, Olibanum, Myrrha, white
The 10 use frequencies of kinds of traditional Chinese medicines such as the root of Dahurian angelica, the Pseudobulbus Bletillae (Rhizoma Bletillae) are the highest, wherein Borneolum Syntheticum, Radix Sanguisorbae, Radix Arnebiae (Radix Lithospermi), Radix Et Rhizoma Rhei, Rhizoma Coptidis,
Cortex Phellodendri, Rhizoma Polygoni Cuspidati have heat-clearing toxin-expelling functions, and Olibanum, Myrrha have blood circulation promoting and blood stasis dispelling, detumescence and promoting granulation effect, in vain
The root of Dahurian angelica has alleviating pain and detumescence effect, and the Pseudobulbus Bletillae (Rhizoma Bletillae) has detumescence and promoting granulation effect, etc..
Owing to the common bacteria of burn infection constantly changes, the difference of different regions medication in addition, pathogen is to medicine
The sensitivity of thing also has difference, so depending on clinical application is intended to as the case may be.Western medicine (chemical drugs)
Effect to burn and scald is antiinflammatory, infection, granulation promoting, promotes wound healing, promotes epithelial growth etc.,
Including substantial amounts of antibacterials such as penicillin, cephalo, sulfadiazine etc., additionally, Western medicine is for little area
Burn and scald therapeutic effect is quick, but then seems unable to do what one wishes for large-area treatment, exist cost high and
The shortcoming that side effect is strong.Washington burn center Durtschi etc. find, the conventional burns using penicillin
People fails to reduce G in early days+Bacterium cellulitis, Wickman finds all use penicillins 5～7 days persons,
The G of drug resistance can be inspired quickly-Bacillus infection.Silver sulfadiazine is widely used antibiotic external used medicine, and it is right
Control traumatic infection and have remarkable result, but the activity of cell is also had certain detrimental effect, especially with
The flamazine paste of high concentration or suspensoid, have and make wound surface damage the fraud increased the weight of.
Chinese herbal medicine is of a great variety, and effect is not quite similar, and is respectively arranged with its unique advantage, and clinic should be each according to burn and scald
The feature in individual period, selects the Chinese herbal medicine of not same-action, the most constantly summing up experience, carries out extensively going deep into
Experimentation, find good effect, low cost, Chinese medicine preparation that toxic and side effects is little.
Callicarpa nudiflora (Callicarpa nudiflora Hook.et Arn.) is Verenaceae Callicarpa plant, is me
The characteristic ethnic drug that state's Li Minority Nationality In Hainan Province area is conventional, saving ground in Guangdong and Guangxi etc. also has distribution, with Hainan
The five fingers mountain products for top grade.Callicarpa nudiflora is used as medicine with ground drying nest, have hemostasis and anti-inflammation, dissipating blood stasis for subsidence of swelling,
Removing toxic substances, effect that wind dispelling is wet, among the people being mainly used in treats wound hemorrhage, respiratory tract and digestive tract hemorrhage, change
The diseases such as purulent inflammation, burn and traumatic hemorrhage are controlled in external.Research to Callicarpa nudiflora now is mainly concentrated
The aspects such as the protection of discovery, hemostatic function and agent for stanching at styptic activity position.
Pollen Typhae (Pollen Typhae) is Typhaceae plant Typha angustifolia Herba Typhae Typha angustifolia L. typha orientalis
Typha orientalis Presl or the dry pollen of congener.Pollen Typhae is classical Chinese medicine, Shennong's Herbal
It is classified as top grade, sweet in the mouth, micro-pungent, cool in nature, there are the effects such as cooling blood for hemostasis, blood circulation promoting and blood stasis dispelling, pain relieving, cure mainly blood
Amenorrhea caused by the stasis of blood, dysmenorrhea, the stagnant stomachache of the stasis of blood in puerperal, traumatic injury and various go out blood disorder.Modern study is thought,
Flavonoid glycoside compound is that Pollen Typhae performance improves microcirculation, the main active substances stopped blooding, restrain.
Summary of the invention
It is an object of the invention to provide a kind of Folium Callicarpae Formosanae Pollen Typhae treatment liquid treating burn and scald and preparation method thereof, will
Callicarpa nudiflora and Pollen Typhae carry out scientific compatibility, synergism, and extracted Aqueous extracts active substance yield is high,
Through clinical observation, this Aqueous extracts has significant external-used burn and scald treating effect, has the huge of clinical practice
Big potentiality.
A kind of Folium Callicarpae Formosanae Pollen Typhae treatment liquid treating burn and scald, is Callicarpa nudiflora and Pollen Typhae to be carried out mixing water carry,
Extracting solution is concentrated into every milliliter of extracting solution and contains Callicarpa nudiflora crude drug 0.08～0.12g, obtains Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts；
Water-bath after being mixed with chitosan solution by Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, filters, and collects clear liquor and obtains Folium Callicarpae Formosanae Pollen Typhae
Treatment liquid, wherein the weight ratio of Callicarpa nudiflora and Pollen Typhae is: Callicarpa nudiflora: Pollen Typhae=10:1～3；Described shell gathers
Sugar juice is to add Chitosan powder to dissolve in 1% glacial acetic acid solution and prepare.
The preparation method of a kind of above-mentioned Folium Callicarpae Formosanae Pollen Typhae treatment liquid, it specifically comprises the following steps that
1) take Callicarpa nudiflora and Pollen Typhae respectively by proportioning, be nakedflower by mass volume ratio after Callicarpa nudiflora is pulverized
Folium Callicarpae Formosanae: the ratio of water=1:15～20 soaks 2～4h, adds Pollen Typhae, heating and refluxing extraction, filters；
Heating and refluxing extraction 2～4 times repeatedly, united extraction liquid, it is concentrated into every milliliter of extracting solution crude drug Han Callicarpa nudiflora
0.08～0.12g, obtain Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts.
2) taking Chitosan powder to add in 1% glacial acetic acid solution, being made into chitosan-containing mass fraction is 1～3%
Solution (chitosan/1% acetic acid=1～3%, w/v), stand 20～25h, filter and i.e. obtain chitosan solution.
3) adding chitosan solution in Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, the addition of chitosan solution is counted by volume
For 8～12%, 60～70 DEG C of water-bath of Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, 100r/min, stir 1～3h, stand 12～
15h。
4) filter, collect clear liquor, subpackage, sterilizing, thus obtaining the product Folium Callicarpae Formosanae Pollen Typhae liquid.
Preparation technology of the present invention is simple, carries out scientific compatibility with Callicarpa nudiflora and Pollen Typhae for raw material, synergism,
Utilizing heating and refluxing extraction, in Aqueous extracts, the kind of effective ingredient is many, and total active constituent content is high, has aobvious
The external-used burn and scald treating effect write, has low cost, activity substance content is high, treatment burn and scald is effective
Etc. feature, there is the great potential of clinical practice.
Accompanying drawing explanation
Fig. 1 is animal subject the 1st day and the 12nd day wound surface comparison figure.
Fig. 2 is the 12nd day each treated animal pathological section comparison figure.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.Hereinafter implement
Example is used for illustrating the present invention, but is not limited to the scope of the present invention.
One, the preparation of Folium Callicarpae Formosanae Pollen Typhae treatment liquid
Embodiment one
Callicarpa nudiflora 1.0kg, pulverizes, and with 15L water soaking 2h, adds Pollen Typhae 100g, and 100 DEG C add
Circumfluence distillation 1h, filters, and filtering residue adds 15L water heating and refluxing extraction 2 times again, each 1h, merges
Three filtrates, heating under reduced pressure backflow is concentrated into every milliliter of Aqueous extracts crude drug 0.1g Han Callicarpa nudiflora, obtains 10L
Aqueous extracts.Add 1L chitosan solution (chitosan/1% acetic acid=1%, w/v), 65 DEG C of water-baths, 100r/min
Stirring 1h, clarifies 12h, filters, and subpackage sterilizing obtains Folium Callicarpae Formosanae Pollen Typhae treatment liquid 8L (crude drug content 0.11g/mL).
Embodiment two
Callicarpa nudiflora 1.0kg, pulverizes, and with 20L water soaking 2h, adds Pollen Typhae 200g, and 100 DEG C add
Circumfluence distillation 2h, filters, and filtering residue adds 20L water heating and refluxing extraction 2 times again, each 2h, merges
Three filtrates, heating under reduced pressure backflow is concentrated into every milliliter of Aqueous extracts crude drug 0.09g Han Callicarpa nudiflora, obtains 13
L Aqueous extracts.Addition 1.5L chitosan solution (chitosan/1% acetic acid=3%, w/v), 70 DEG C of water-baths, 100
R/min stirs 1h, clarifies 14h, filters, and subpackage sterilizing obtains Folium Callicarpae Formosanae Pollen Typhae treatment liquid 12L, and (crude drug contains
Amount 0.10g/mL).
Embodiment three
Callicarpa nudiflora 1.0kg, pulverizes, and with 18L water soaking 2h, adds Pollen Typhae 250g, and 100 DEG C add
Circumfluence distillation 1h, filters, and filtering residue adds 18L water heating and refluxing extraction 2 times again, each 1h, merges
Three filtrates, heating under reduced pressure backflow is concentrated into every milliliter of aqueous extract crude drug 0.12g Han Callicarpa nudiflora, obtains
12L Aqueous extracts.Addition 1.3L chitosan solution (chitosan/1% acetic acid=2%, w/v), 60 DEG C of water-baths,
100r/min stirs 1h, clarifies 12h, filters, and it is (raw that subpackage sterilizing obtains Folium Callicarpae Formosanae Pollen Typhae treatment liquid 11L
Medicine content 0.12g/mL).
Two, Folium Callicarpae Formosanae Pollen Typhae liquid bacteriostatic activity is observed
Use fluid medium method to measure MIC, investigate Folium Callicarpae Formosanae Pollen Typhae treatment liquid with Callicarpa nudiflora decocting liquid to burning
Scald the antibacterial activity of clinical common separation of bacterial.
1. test liquid configuration: take sterilizing test tubes 10, be separately added into 2mL meat soup fluid medium numbered
1-10, takes concentrated medicament 2ml and adds No. 1 test tube, mixing, take 2ml and add No. 2 pipes from No. 1 pipe,
Diluting successively, in the 10th pipe, sucking-off 2ml discards.Take No. 11 test tubes addition 2ml meat soup fluid mediums to do
Blank.
2. strain prepares: take each bacterium nutrient broth culture cultivated 18 hours, with meat soup by turbid for this bacterium stock solution
Degree is adjusted to optically be equivalent to 0.5 Maxwell standard pipe (available photometer or range estimation), 0.5 Maxwell standard
Culture is containing about 1～2 × 108CFU/ml, can get antibacterial by 10 times of dilution 0.5 Maxwell standard bacterium solution and applies
Liquid.The strain application liquid obtained is tried one's best and is used in 15min.
Take 0.1ml strain application liquid the most respectively and add in 1～No. 11 small test tube, 37 DEG C of calorstats are cultivated 18
Hour.More each pipe growth situation, as opaque by reagent color, it is impossible to compare, then use inoculating loop
From each test tube, take a ring be inoculated into respectively on MH plate, cultivate 18 hours and observe with or without bacterial growth, with
The minimum inhibitory concentration (MIC) that the highest drug dilution degree is this medicine that antibacterial does not grows.The antibacterial observed result of MIC
It is shown in Table 1.
Table 1 Folium Callicarpae Formosanae Pollen Typhae liquid compares (mg/mL, n=3) with Callicarpa nudiflora decocting liquid fungistatic effect
4. bacteriostatic activity is according to the antibacterial observed result of MIC (being shown in Table 1), shows more traditional naked of Folium Callicarpae Formosanae Pollen Typhae liquid
The Antibacterial Activity of micrococcus scarlatinae, escherichia coli and Pseudomonas aeruginosa is strengthened by flower Folium Callicarpae Formosanae decocting liquid, right
Staphylococcus aureus and salmonella typhi vigor are suitable.At whole prevention with treatment burn and scald postoperative infection also
Send out disease clinical effectiveness to strengthen.
Three, Folium Callicarpae Formosanae Pollen Typhae treatment liquid treatment burn and scald observation of curative effect
1. laboratory animal: Male New Zealand rabbits 20, weight 2～2.5kg, the laboratory animal quality certification number:
44005900002274, the laboratory animal use quality certification number: 44005900002274, by Guangzhou Chinese medicine
University's Experimental Animal Center provides.
2. experimental drug: Sulfadiazine Silver Cream, is provided by Hua Xin pharmaceutcal corporation, Ltd of Xinxiang City, lot number:
150306；Chloral hydrate, is provided by Tianjin Kermel Chemical Reagent Co., Ltd., lot number: 20130325,
It is configured to 10% chloral hydrate solution with distilled water；Su Mian Xin injection, is reached animal by Dunhua of Jilin Province city sage
Medicine company limited provides, lot number: 150128；The Folium Callicarpae Formosanae Pollen Typhae treatment liquid that embodiment one provides.
3. modelling and packet: before experiment starts, new zealand rabbit back shaving, with Su Mian Xin and chloral hydrate
Mixed fiber crops, clamp, with long friction clamp, 64 layers of gauze that area is 3cm × 3cm and immerse 100 DEG C of boiled water 5 minutes, take
Go out, be immediately placed on area skin to be scalded, start timing 15 seconds after the crystal of upper pressure 500g mass, after modeling
Single cage is raised.
Animal packet and process:
Blank group (3): do not make scald and process；
Model group (7): scald, be not administered, after the 1st modeling, execution 2 is drawn materials and is made HE dyeing
Observe, after the 2nd modeling, take 1 rabbit biopsy, take scald position and make HE dyeing observation；
Sulfadiazine Silver Cream positive group (call in the following text positive group, 5): every in the sky, noon smears emulsifiable paste each 1 time,
Dosage about 2g, continuous 12 days；
Folium Callicarpae Formosanae Pollen Typhae treatment liquid is for examination group (call in the following text and supply examination group, 5): the upper and lower noon respectively creates with Chinese medicine spray
Scalding position, Chinese medicine is completely covered wound surface, continuous 12 days；
Detection: taking pictures when every day changes dressings and observe into crust situation, under crust, hydrops can use low level opening drain, protects
Hold wound surface to be dried.After treatment terminates, take tissue at edge of wound and make HE dyeing observation.
4. experimental result
(1) gross examination of skeletal muscle: all can normally revive after each treated animal modeling, within the 1st day, appetite is poor, the 2nd day
Starting gradually to recover normal, observe 12 days, animal all survives.Intuitively observe according to wound healing, positive group
It is significantly better than model group with for examination group.Result is shown in Fig. 1.
(2) pathological observation: the 12nd day animal skin tissue carries out pathology section examination to test, and result is shown in
Fig. 2, normal blank matched group, skin overall structure is normal, without obvious pathological changes；Without obvious inflammatory cell
Infiltration and necrosis；Model group skin major part epidermis erosion necrosis comes off, and the chip that comes off seen from periphery is (as shallow
Shown in black arrow), at corium, visible red cell is excessive, accompanies a large amount of inflammatory cell infiltration (such as black arrow institute
Show)；Form a scab on positive controls epiderm skin, follicular hyperkeratosis (as illustrated with black arrow) seen from skin,
A small amount of inflammatory cell infiltration (as shown in light/dark balance arrow) seen from corium；Folium Callicarpae Formosanae Pollen Typhae treatment liquid is for examination group skin
Skin part epidermis is banding (as illustrated with black arrow), and tissue is without other obvious pathological changes, normal.
Result shows: Sulfadiazine Silver Cream positive group and Folium Callicarpae Formosanae Pollen Typhae treatment liquid supply examination group to II degree Scalded Rabbits
Model therapeutic effect is significantly better than model group, and thinks that the treatment liquid group effect of the reparation Folium Callicarpae Formosanae Pollen Typhae to wound surface is excellent
In positive group.
The above is only the preferred embodiment of the present invention, it is noted that common for the art
For technical staff, on the premise of without departing from the technology of the present invention principle, it is also possible to make some improvement and profit
Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (2)
1. treat a Folium Callicarpae Formosanae Pollen Typhae treatment liquid for burn and scald, it is characterized in that mixing Callicarpa nudiflora and Pollen Typhae
Heshui carries, and extracting solution is concentrated into every milliliter of extracting solution and contains Callicarpa nudiflora crude drug 0.08～0.12g, obtains Folium Callicarpae Formosanae Pu
Yellow fluid extract；Water-bath after being mixed with chitosan solution by Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, filters, and collects clear liquor and obtains
Treating liquid to Folium Callicarpae Formosanae Pollen Typhae, wherein the weight ratio of Callicarpa nudiflora and Pollen Typhae is: Callicarpa nudiflora: Pollen Typhae=10:1～3；
Described chitosan solution is to add Chitosan powder to dissolve in 1% glacial acetic acid solution and prepare.
2. the preparation method of the Folium Callicarpae Formosanae Pollen Typhae treatment liquid described in a claim 1, it is characterised in that its tool
Body step is as follows:
1) take Callicarpa nudiflora and Pollen Typhae respectively by proportioning, be nakedflower by mass volume ratio after Callicarpa nudiflora is pulverized
Folium Callicarpae Formosanae: the ratio of water=1:15～20 soaks 2～4h, adds Pollen Typhae, heating and refluxing extraction, filters；
Heating and refluxing extraction 2～4 times repeatedly, united extraction liquid, it is concentrated into every milliliter of extracting solution crude drug Han Callicarpa nudiflora
0.08～0.12g, obtain Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts；
2) taking Chitosan powder to add in 1% glacial acetic acid solution, being made into chitosan-containing mass fraction is 1～3%
Solution, stand 20～25h, filter and i.e. obtain chitosan solution；
3) adding chitosan solution in Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, the addition of chitosan solution is counted by volume
For 8～12%, 60～70 DEG C of water-bath of Folium Callicarpae Formosanae Pollen Typhae Aqueous extracts, 100r/min, stir 1～3h, stand 12～
15h；
4) filter, collect clear liquor, subpackage, sterilizing, thus obtaining the product Folium Callicarpae Formosanae Pollen Typhae liquid.
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Cited By (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109123174A (en) * | 2018-07-25 | 2019-01-04 | 广州格雷特生物科技有限公司 | Containing callicarpa nudiflora poultry premixed feed and its preparation method and application |
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