CN105942131A - Composite clarifying agent for ginger juice and preparation method of extracting solution of clarified ginger juice - Google Patents

Composite clarifying agent for ginger juice and preparation method of extracting solution of clarified ginger juice Download PDF

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CN105942131A
CN105942131A CN201610314359.5A CN201610314359A CN105942131A CN 105942131 A CN105942131 A CN 105942131A CN 201610314359 A CN201610314359 A CN 201610314359A CN 105942131 A CN105942131 A CN 105942131A
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rhizoma zingiberis
zingiberis recens
chitosan
ginger juice
preparation
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CN105942131B (en
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毕艳红
王朝宇
赵祥杰
杨荣玲
陈晓明
蒋长兴
张文静
苗金梁
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Tengshu Biotechnology Jiaxing Co ltd
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Huaiyin Institute of Technology
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/80—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/82—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by flocculation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Nutrition Science (AREA)
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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention relates to a composite clarifying agent for ginger juice and a preparation method of an extracting solution of clarified ginger juice, and belongs to the technical field of food processing. The composite clarifying agent for the ginger juice is characterized in that attapulgite is used for supporting chitosan and matched with sodium alginate, sodium carboxymethyl cellulose, xanthan gum, collagen and tea polyphenol, the composite clarifying agent has excellent flocculation effect as well as good biocompatibility, adaptability and safety, can adsorb suspended solids, pigments and the other substances in the ginger juice more comprehensively and thoroughly, has antiseptic and bacteriostatic functions and can prolong the storage period of the ginger juice effectively. According to the preparation method of the extracting solution of the clarified ginger juice, the content of gingerol in extracted ginger juice is increased greatly while nutrients of raw materials are retained sufficiently, nutrient loss is small, the use amount of the clarifying agent is small, the stability of the ginger juice is improved remarkably, the prepared ginger juice is clear and transparent in appearance and glittering and translucent in color, the pungency of the ginger juice is reduced, the taste is mellow, and the sensory quality is improved obviously.

Description

A kind of compound Sucus Zingberis clarifier and the preparation method of clarification Sucus Zingberis extracting solution
Technical field
The invention belongs to food processing technology field, relate to a kind of compound Sucus Zingberis clarifier and the system of clarification Sucus Zingberis extracting solution Preparation Method.
Background technology
Rhizoma Zingiberis Recens common name Rhizoma Zingiberis Recens, its acrid in the mouth, warm in nature, there is relieving the exterior syndrome by diaphoresis, preventing phlegm from forming and stopping coughing, the effect of appetizing preventing or arresting vomiting, be a kind of wide The medicine food dual purpose plant of general application, in addition to containing the major physiological active substance such as (4-hydroxy-3-methoxyphenyl)ethyl methyl ketone, gingerol, possibly together with protein, many glycosides, Vitamin and various trace elements.Every 100 g fresh ginger edible parts contain protein 1.4 g, fatty 0.7 g, sugared 8.8 g, calcium 20 mg, phosphorus 45 mg, ferrum 7 mg, carotene 0.18 g, vitamin B1 0.01 mg, vitamin C 4 mg and nicotinic acid 0.4 Mg etc..Rhizoma Zingiberis Recens is edible with loose meat rhizome, is one of indispensable important flavoring agent in people's daily life.
China is one of the cradle of Rhizoma Zingiberis Recens and main country of origin, and the China that is planted in of Rhizoma Zingiberis Recens has a long history.20 After century, the most gradually one of leading exporter becoming Rhizoma Zingiberis Recens of China.But the storage of Rhizoma Zingiberis Recens is more difficult, perishable rotten, the most often To the harvest season, Rhizoma Zingiberis Recens loss in producing region is the biggest.Up to now, the trade main body of Rhizoma Zingiberis Recens is still Rhizoma Zingiberis, and the edible many works of Rhizoma Zingiberis Recens are common Flavouring agent, in China always based on former Rhizoma Zingiberis Recens or Rhizoma Zingiberis powder, utilization rate is extremely low.
In recent years, develop multiple Rhizoma Zingiberis Recens health food both at home and abroad, such as ginger ale, ginger tea, ginger vinegar beverage, Sucus Zingberis dairy Product, Milk Coagulation with Ginger Juice, flavor compounds in ginger snack food, glycyrrhizic acid MEIJIANG, Herba Alii fistulosi brittleness Rhizoma Zingiberis etc..Rhizoma Zingiberis Recens juice is widely used in opening of beverage Send out and produce, but the beverage become for Raw material processing with Rhizoma Zingiberis Recens at present is made by edible embedding medium or spray drying method mostly Solid ginger beverage.Clear appearance is bright, color and luster is sparkling and crystal-clear, pure and fresh tasty and refreshing clarification type ginger juice drink phoenix feathers and unicorn horns.
The present invention tests discovery Rhizoma Zingiberis Recens clarification of juice to keeping system to stablize and to guarantee that the quality of product has the heaviest The meaning wanted.Current domestic usual employing enzyme process carries out Rhizoma Zingiberis Recens clarification of juice, utilizes the enzyme preparation such as pectase, amylase to be hydrolyzed Fruit juice can cause pectin substance and the polysaccharide of muddiness, make other colloid in fruit juice lose the protective effect of pectin and common Precipitation, thus reach the purpose of clarification, improve production efficiency and crushing juice rate simultaneously, to keeping, nutritional labeling is the most highly beneficial. Pectase preparation is the general name decomposing the multiple enzyme of pectin, mainly has pectinesterase (PE), polygalacturonase (PG) etc., dry enzyme Preparation consumption is generally 2 ~ 4 g/L, reaction temperature 45 ~ 55 DEG C, response time 1 ~ 3 h.The optimal work of enzymolyzing alpha-amylase starch Skill condition is: the pH first adjusting the Rhizoma Zingiberis Recens natural juice after gelatinizing is 4, then by the amount of 0.2 mL/L, isothermal holding at a temperature of 60 DEG C 30 min。
But the shortcomings such as operation is complicated, cost is high, clarifying effect difference that enzyme process clarification Sucus Zingberis also exists, Sucus Zingberis is the most quiet simultaneously Easily polluted by microorganism in putting and ferment rotten, it is impossible to ensure its quality, it is necessary to add suitable preservative.
Wang Jihong, Kong Fandong etc. use gelatin tannin fining process, are shown Sucus Zingberis clarification experiments by gelatin, tannin: when Gelatin addition is 0.04 ‰, and tannin addition is to act on 1 h under 0.07 ‰, room temperature, and light transmittance is 98.6%, but color with Stability is relatively poor, affects greatly sense organ, and easily occurs secondary muddy.
Therefore, by Sucus Zingberis clarifier and clarification Sucus Zingberis Study on extraction, preparing limpid bright steady quality and energy Keep the Sucus Zingberis product of the active constituent contents such as gingerol to greatest extent, be necessary to widening Rhizoma Zingiberis Recens product market, simultaneously Also the comprehensive development and utilization for Rhizoma Zingiberis Recens provides new approaches.
Summary of the invention
The present invention is directed in prior art, extract and the problems referred to above present in clarification process at Sucus Zingberis, it is provided that Yi Zhongfu Close Sucus Zingberis clarifier, additionally provide the preparation method of a kind of clarification Sucus Zingberis extracting solution using this compound Sucus Zingberis clarifier simultaneously.
Technical solution of the present invention is as follows:
A kind of compound Sucus Zingberis clarifier, is mixed by the raw material of following weight portion: attapulgite clay loading chitosan 25～35, alginic acid Sodium 15～20, sodium carboxymethyl cellulose 11～22, xanthan gum 13～16, collagen 5～8 and tea polyphenols 0.5～1；
The preparation method of described attapulgite clay loading chitosan, step includes:
1) attapulgite clay being calcined at 700 DEG C 4 h, after cooling, join in the aqueous hydrochloric acid solution of 1 mol/L, attapulgite clay is at hydrochloric acid water In solution, concentration is 0.5 g/mL, and at 90 DEG C, constant temperature stirs 4 h, is then centrifuged for, and collects solid and is washed to neutrality, being subsequently adding In the oxalic acid aqueous solution that mass fraction is 10%, addition is 0.5 g/mL, 2 hs is stirred at room temperature and stands 5 h, centrifugation, receive Collection solid water is washed till neutrality, and 120 DEG C of drying obtain acid activation attapulgite clay；
2) 10wt% oxalic acid aqueous solution and 1wt% acetic acid aqueous solution are mixed for 4:1 by volume, be subsequently adding solution quality 3% Chitosan, speed of agitator 50～60rpm in adition process, then solution is heated to 50 DEG C and is incubated to chitosan the most molten Solve, obtain faint yellow viscid chitosan colloidal sol；
3) take chitosan colloidal sol, dilute with the water of 2.5 times of volumes, be heated to 50 DEG C of stirring 1 h, be then down to room temperature, by 5 g/ Ml concentration adds acid activation attapulgite clay, stirs 5 h, and centrifugal, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain attapulgite clay chitosan；
4) again take the chitosan colloidal sol with step 3) same volume, dilute with the water of 1 times of volume, add step 3) load and be dried After attapulgite clay chitosan, 50 DEG C of stirring 6 h, stand, remove supernatant, in residue, add chitosan colloidal sol 2 times of volumes The NaOH aqueous solution of 1.0 mol/L, after stirring 1 h, centrifugal, it is washed to neutrality, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain light The attapulgite clay loading chitosan of yellow.
A kind of preparation method clarifying Sucus Zingberis extracting solution, uses above-mentioned compound Sucus Zingberis clarifier, comprises the following steps:
1) fresh ginger cleaned, cut into slices, add colour fixative, be dried, be then crushed to the Rhizoma Zingiberis powder of 40-60 mesh, add 3 times of quality Water, moistening mixing, expanded through twin-screw extruder, be crushed to 100-200 mesh, obtain expanded Rhizoma Zingiberis powder；
2) the expanded Rhizoma Zingiberis powder of gained in step 1) is mixed to obtain Rhizoma Zingiberis Recens slurry with water according to the mass ratio of 1:20, be added thereto to The pectase of 0.006wt% and the cellulase of 0.004wt%, enzymolysis 30～60min at initial p H value 5.5,40 DEG C；Then Adding the glucoamylase of 0.012wt%, enzymolysis 30min～120min at original ph 4.5,55 DEG C, enzymolysis rises after completing Temperature, to 80 C insulations 6～10min, enzyme denaturing, cooling, obtains enzymolysis Rhizoma Zingiberis Recens slurry；
3) to step 2) gained enzymolysis Rhizoma Zingiberis Recens slurry in add 1～2wt% organic cellulose as filter aid agent, mix homogeneously, 100 mesh squeezing Filter, collect Rhizoma Zingiberis Recens liquid and Rhizoma Zingiberis Recens dregs respectively；
4) in the Rhizoma Zingiberis Recens liquid of step 3), adding 0.5～2wt% and be combined Sucus Zingberis clarifier, ultrasonic 5-60min, through 5000 r/min Centrifugal 20～30 min, obtain clarification Sucus Zingberis；
5) step 3) gained Rhizoma Zingiberis Recens dregs 60 DEG C is dried, with the ethanol water of 60 wt % as extractant, and solid-liquid ratio 1:15g/mL, At ultrasonic power 120W, ultrasonic temperature 40 DEG C, extract 15min, filter, collect filtrate, rotated evaporate to obtain Rhizoma Zingiberis Recens extract；
6) in the clarification Sucus Zingberis of step 4) gained, add the Rhizoma Zingiberis Recens extract of 0.01～0.1wt%, mix homogeneously, Rhizoma Zingiberis Recens must be clarified Juice extracting solution.
Described, in step 1) Rhizoma Zingiberis Recens slice size be 0.5～1.0cm, thickness 0.2～0.3cm；
Described, in step 1), colour fixative is the aqueous citric acid solution of pH4.5～5.5,0.02-0.10wt% sodium ascorbate water One or several mixing in the distannous citrate aqueous solution of solution and 0.015-0.15wt%；
Described, in step 1) the jacket temperature of twin-screw extruder be 40～45 DEG C, screw speed be 100～120r/ Min, die orifice aperture are 5～10mm；
Described, in step 4), ultrasound condition is supersonic frequency 0.1～1.0MHz, temperature 10～50 DEG C；
Described, in step 4), compound Sucus Zingberis clarifier composition is attapulgite clay loading chitosan, sodium alginate, carboxymethyl cellulose Sodium, collagen and tea polyphenols.
The compound Sucus Zingberis clarifier that the present invention provides, with attapulgite clay loading chitosan as main component, with the use of alginic acid Sodium, sodium carboxymethyl cellulose, xanthan gum, collagen and tea polyphenols.Attapulgite clay has special fibre structure and unusual suction Attached performance, has decoloring ability simultaneously, and the effect being acidified attapulgite clay removal invalid components is better than common attapulgite clay.Chitosan is sky Right cationic flocculant, has excellent flocculating effect, has good biocompatibility, adaptability and safety simultaneously Property.Compounding with acidifying attapulgite clay and chitosan, clarify for Sucus Zingberis, attapulgite clay is combined with chitosan can be electronegative with sour environment Soluble protein, cellulose, pectin, particle, the material such as tannin there is the strongest agglutination.It addition, by adding The auxiliary elements such as the sodium alginate, sodium carboxymethyl cellulose, xanthan gum, collagen and the tea polyphenols that enter, assist with attapulgite clay loading chitosan Same potentiation, sodium alginate, sodium carboxymethyl cellulose and xanthan gum etc. form co-agglomeration thing, and form complex with collagen, can make Sucus Zingberis causes the abundant coagulating sedimentations such as muddy albumen, removes tannin class impurity, thoroughly adsorb hanging in Sucus Zingberis more comprehensively Float and pigment etc., improve product yield and quality, shortens settling time, reduces cost, safe and reliable, the most also has anticorrosion Bacteriostasis, can extend Sucus Zingberis storage period effectively.
In the preparation method of the clarification Sucus Zingberis extracting solution that the present invention provides, extruding and puffing technology is by high temperature, high pressure, height The effect sheared, makes the starch gelatinization in Rhizoma Zingiberis powder, protein denaturation, adipose cell rupture, cellulose ruptures, to having health care merit Energy, of a relatively high to hot more sensitive nutritional labeling retention rate, beneficially the carrying out of following process.By glucoamylase, really The enzymolysis processing such as glue enzyme, cellulase and the present invention be combined Sucus Zingberis clarifier with the use of, the intercellular layer of Rhizoma Zingiberis Recens cell wall is main For pectic substance, primary wall be mainly composed of cellulose, hemicellulose, secondary wall be mainly composed of cellulose, pectase can So that the macromole Pectin, proto of long-chain resolves into small soluble molecules pectin, galacturonic acid oligosaccharides and the galacturonic acid of short chain Acid, cellulase energy catalyzing cellulose hydrolysis, make cellulose solubilising and saccharifying, glucoamylase can be by the glucosides in liquid starch Key is progressively hydrolyzed to the oligosaccharide such as glucose, dextrinose, panose.Pectase and cellulase is selected to carry out enzymolysis the most respectively Destroying the intercellular layer of cell wall, to remove the cellulose outer wall of cell, glucose starch enzymatic conversion Sucus Zingberis easily causes muddiness Starch and protein component, obtain the exposed protoplast dissociated, thus improve the crushing juice rate of Rhizoma Zingiberis Recens.In preparation method, to Rhizoma Zingiberis Recens Slag uses vegetable oil and ethanol extraction, makes the nutritional labeling gingerol etc. being mainly distributed in Cortex Zingiberis be fully dissolved out, to greatest extent Remain Sucus Zingberis effective ingredient, after clarifying treatment, then use glucoseoxidase enzymolysis, gluconic acid can be converted glucose into, Produce hydrogen peroxide simultaneously, the oxygen in Sucus Zingberis can be removed, thus be effectively prevented rotten, removal natural pigment, anti-corrosive fresh-keeping, Also can increase Sucus Zingberis deliciousness flavour.
Technical solution of the present invention has the beneficial effect that
1) present invention is combined Sucus Zingberis clarifier, compounds, with the use of sodium alginate, carboxymethyl cellulose with attapulgite clay and chitosan Sodium, xanthan gum, collagen and tea polyphenols, have excellent flocculating effect, has good biocompatibility, adaptability and safety Property.More comprehensive can thoroughly adsorb the float in Sucus Zingberis and pigment etc., the albumen etc. causing muddiness in Sucus Zingberis can be made fully to coagulate Knot precipitation, removes tannin class impurity, improves product yield and quality, shortens settling time, reduces cost, safe and reliable, simultaneously Also there is antiseptic and inhibiting bacteria function effect, can effectively extend Sucus Zingberis storage period.
2) present invention clarifies the preparation method of Sucus Zingberis extracting solution, significantly carries while being sufficiently reserved each nutritional labeling of raw material The content of gingerol in the high Sucus Zingberis extracted, nutrient substance runs off few, and clarifier consumption is low, Sucus Zingberis is greatly improved stable Property, the Sucus Zingberis clear appearance of preparation is bright, color and luster is sparkling and crystal-clear and reduces the pungent sense of Sucus Zingberis, and mouthfeel is aromatic, and aesthetic quality is obvious Improve.
Detailed description of the invention
Below by embodiment and comparative example further illustrate technical solution of the present invention beneficial effect.
Used by the present invention, enzyme is food stage, purchased from Jiangsu Ruiyang Biological Technology Co., Ltd., if other raw material nothing Special instruction, is also market and buys.
Embodiment 1
A kind of compound Sucus Zingberis clarifier, is mixed by the raw material of following weight portion: attapulgite clay loading chitosan 30, sodium alginate 18, sodium carboxymethyl cellulose 16, xanthan gum 15, collagen 6 and tea polyphenols 0.7；
The preparation method of described attapulgite clay loading chitosan, step includes:
1) attapulgite clay being calcined at 700 DEG C 4 h, after cooling, join in the aqueous hydrochloric acid solution of 1 mol/L, attapulgite clay is at hydrochloric acid water In solution, concentration is 0.5 g/ml, and at 90 DEG C, constant temperature stirs 4 h, is then centrifuged for, and collects solid and is washed to neutrality, being subsequently adding In the oxalic acid aqueous solution that mass fraction is 10%, addition is 0.5 g/ml, 2 hs is stirred at room temperature and stands 5 h, centrifugation, receive Collection solid water is washed till neutrality, and 120 DEG C of drying obtain acid activation attapulgite clay；
2) 10wt% oxalic acid aqueous solution and 1wt% acetic acid aqueous solution are mixed for 4:1 by volume, be subsequently adding solution quality 3% Chitosan, speed of agitator 50～60rpm in adition process, then solution is heated to 50 DEG C and is incubated to chitosan the most molten Solve, obtain faint yellow viscid chitosan colloidal sol；
3) take chitosan colloidal sol, dilute with the water of 2.5 times of volumes, be heated to 50 DEG C of stirring 1 h, be then down to room temperature, by 5 g/ Ml concentration adds acid activation attapulgite clay, stirs 5 h, and centrifugal, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain attapulgite clay chitosan；
4) again take the chitosan colloidal sol with step 3) same volume, dilute with the water of 1 times of volume, add step 3) load and be dried After attapulgite clay chitosan, 50 DEG C of stirring 6 h, stand, remove supernatant, in residue, add chitosan colloidal sol 2 times of volumes The NaOH aqueous solution of 1.0 mol/L, after stirring 1 h, centrifugal, it is washed to neutrality, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain light The attapulgite clay loading chitosan of yellow.
Embodiment 2
A kind of compound Sucus Zingberis clarifier, is mixed by the raw material of following weight portion: attapulgite clay loading chitosan 25, sodium alginate 20, sodium carboxymethyl cellulose 11, xanthan gum 13, collagen 5 and tea polyphenols 0.5；
Embodiment 3
A kind of compound Sucus Zingberis clarifier, is mixed by the raw material of following weight portion: attapulgite clay loading chitosan 35, sodium alginate 15, sodium carboxymethyl cellulose 22, xanthan gum 16, collagen 8 and tea polyphenols 1.
Comparative example 1
A kind of compound clarifier, is mixed by the raw material of following weight portion: attapulgite clay 30, chitosan 30, sodium alginate 18, carboxylic first Base sodium cellulosate 16, xanthan gum 15, collagen 6 and tea polyphenols 0.7；
Clarifier Contrast on effect: each 100g of Rhizoma Zingiberis Recens liquid after pressure squeezing process, is separately added into the clear of the embodiment of the present invention 1～3 preparation Clear agent and the clarifier for contrast, clarifier consumption is 2g, and clarifying effect is shown in Table 1.
The different clarifier comparison to Sucus Zingberis clarifying effect of table 1
Embodiment 4
A kind of preparation method clarifying Sucus Zingberis extracting solution, uses the compound Sucus Zingberis clarifier of embodiment 1, comprises the following steps:
1) being cleaned by fresh ginger, cut into slices, size is 0.5～1.0cm, thickness 0.2～0.3cm, and adding volume ratio is 1:1's Aqueous citric acid solution (pH4.5～5.5) and the mixing colour fixative of ascorbic acid sodium water solution (0.02～0.10wt%), be dried, so After be crushed to the Rhizoma Zingiberis powder of 40～60 mesh, add the water of 3 times of quality, moistening mixing, expanded through twin-screw extruder, jacket temperature is 40 ～45 DEG C, screw speed be 100～120r/min, die orifice aperture be 5～10mm, be then crushed to 100～200 mesh, obtain expanded Rhizoma Zingiberis powder；
2) the expanded Rhizoma Zingiberis powder of gained in step 1) is mixed to obtain Rhizoma Zingiberis Recens slurry with water according to the mass ratio of 1:20, be added thereto to The pectase of 0.006wt% and the cellulase of 0.004wt%, enzymolysis 30～60min at initial p H value 5.5,40 DEG C；Then Adding the glucoamylase of 0.012wt%, enzymolysis 30min～120min at original ph 4.5,55 DEG C, enzymolysis rises after completing Temperature, to 80 C insulations 6～10min, enzyme denaturing, cooling, obtains enzymolysis Rhizoma Zingiberis Recens slurry；
3) to step 2) gained enzymolysis Rhizoma Zingiberis Recens slurry in add 1～2wt% organic cellulose as filter aid agent, mix homogeneously, 100 mesh squeezing Filter, collect Rhizoma Zingiberis Recens liquid and Rhizoma Zingiberis Recens dregs respectively；
4) in the Rhizoma Zingiberis Recens liquid of step 3), add 0.5～2wt% and be combined Sucus Zingberis clarifier (being prepared by embodiment 1), ultrasonic 5～ 60min, supersonic frequency 0.1～1.0MHz, temperature 10～50 DEG C, it is centrifuged 20～30 min through 5000 r/min, obtains clarification Sucus Zingberis；
5) step 3) gained Rhizoma Zingiberis Recens dregs 60 DEG C is dried, with the ethanol water of 60 wt % as extractant, and solid-liquid ratio 1:15(g/ ML), extracting 15min at ultrasonic power 120W, ultrasonic temperature 40 DEG C, filter, collection filtrate, rotated evaporation to obtain Rhizoma Zingiberis Recens extraction Thing；
6) in the clarification Sucus Zingberis of step 4) gained, add the Rhizoma Zingiberis Recens extract of 0.01～0.1wt%, mix homogeneously, Rhizoma Zingiberis Recens must be clarified Juice extracting solution.
Embodiment 5
The preparation method of clarification Sucus Zingberis extracting solution, uses the compound Sucus Zingberis clarifier of embodiment 2, and step is with embodiment 4.
Embodiment 6
The preparation method of clarification Sucus Zingberis extracting solution, uses the compound Sucus Zingberis clarifier of embodiment 3, and step is with embodiment 4.
Comparative example 2
The preparation method of clarification Sucus Zingberis extracting solution, step 2) it is simultaneously introduced pectase, cellulase, glucoamylase, addition Constant, enzymolysis 1h at initial p H value 5.5,50 DEG C, other step is with embodiment 4.
Comparative example 3
The preparation method of clarification Sucus Zingberis extracting solution, after step 3) squeeze and filter, Rhizoma Zingiberis Recens dregs processes without step 4) and directly abandons, other Step is with embodiment 4.
Embodiment 4～6 and the preparation method Contrast on effect of comparative example 2～3 clarification Sucus Zingberis extracting solution, effect is shown in Table 2:
The comparison of the preparation method effect of table 2 different clarification Sucus Zingberis extracting solution
From table 1, different clarifiers has larger difference to the clarifying effect of Sucus Zingberis.Comparative example 1 and single concavo-convex load Chitosan, purification attapulgite clay, acidifying attapulgite clay, its clarifying effect is below the compound clarifier in embodiment 1-3.This is due to compound Clarifier is after compounding, and its synergism is much larger than single clarifier.In table 2, in comparative example 2, in enzymolysis process, a step is enzyme-added Carrying out enzymolysis, its crushing juice rate decreases compared with embodiment 4-6, this is because, a step adds enzyme process and cannot realize every kind of enzyme and exist Hydrolysis result under its optimum condition；Comparative example 3 is then that Rhizoma Zingiberis Recens dregs is not directly dropped by further effective component extracting, wherein Local flavor effective ingredient not through Hui Tian, cause in gained clear juice Rhizoma Zingiberis Recens local flavor not enough, also result in the waste of resource in addition.And After the process of method described in embodiment 4-6, crushing juice rate and the contained effective ingredient of Sucus Zingberis all have raising by a relatively large margin.Contrast Table 1, table 2 are it is found that in embodiment and comparative example, the process of Rhizoma Zingiberis Recens is direct extracting juice in table 1, and Rhizoma Zingiberis Recens in embodiment in table 2 Process for be dried expanded after carry out enzymolysis extracting juice.Contained nutritional labeling from clarification Sucus Zingberis is it can be seen that through expanded enzymolysis After Rhizoma Zingiberis Recens gained clear juice Middle nutrition composition more horn of plenty, the particularly content of soluble solid improves 4-5 times, affects clear The content of the protein that clear juice is stable decreases, and substantially increases the stability of Rhizoma Zingiberis Recens subsider juice.

Claims (7)

1. a compound Sucus Zingberis clarifier, it is characterised in that mixed by the raw material of following weight portion: attapulgite clay loading chitosan 25～35, sodium alginate 15～20, sodium carboxymethyl cellulose 11～22, xanthan gum 13～16, collagen 5～8 and tea polyphenols 0.5～ 1；
The preparation method of described attapulgite clay loading chitosan, step includes:
1) attapulgite clay being calcined at 700 DEG C 4 h, after cooling, join in the aqueous hydrochloric acid solution of 1 mol/L, attapulgite clay is at hydrochloric acid water In solution, concentration is 0.5 g/mL, and at 90 DEG C, constant temperature stirs 4 h, is then centrifuged for, and collects solid and is washed to neutrality, being subsequently adding In the oxalic acid aqueous solution that mass fraction is 10%, addition is 0.5 g/mL, and 2 hs are stirred at room temperature, and stands 5 h, centrifugation, Collect solid water and be washed till neutrality, 120 DEG C of drying, obtain acid activation attapulgite clay；
2) 10wt% oxalic acid aqueous solution and 1wt% acetic acid aqueous solution are mixed for 4:1 by volume, be subsequently adding solution quality 3% Chitosan, speed of agitator 50～60rpm in adition process, then solution is heated to 50 DEG C and is incubated to chitosan the most molten Solve, obtain faint yellow viscid chitosan colloidal sol；
3) take chitosan colloidal sol, dilute with the water of 2.5 times of volumes, be heated to 50 DEG C of stirring 1 h, be then down to room temperature, by 5 g/ Ml concentration adds acid activation attapulgite clay, stirs 5 h, and centrifugal, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain attapulgite clay chitosan；
4) again take the chitosan colloidal sol with step 3) same volume, dilute with the water of 1 times of volume, add step 3) load and be dried After attapulgite clay chitosan, 50 DEG C of stirring 6 h, stand, remove supernatant, in residue, add chitosan colloidal sol 2 times of volumes The NaOH aqueous solution of 1.0 mol/L, after stirring 1 h, centrifugal, it is washed to neutrality, sucking filtration, 55 DEG C are dried under vacuum to constant weight, obtain light The attapulgite clay loading chitosan of yellow.
2. the preparation method clarifying Sucus Zingberis extracting solution, it is characterised in that comprise the following steps:
1) fresh ginger cleaned, cut into slices, add colour fixative, be dried, be then crushed to the Rhizoma Zingiberis powder of 40-60 mesh, add 3 times of quality Water, moistening mixing, expanded through twin-screw extruder, be crushed to 100-200 mesh, obtain expanded Rhizoma Zingiberis powder；
2) the expanded Rhizoma Zingiberis powder of gained in step 1) is mixed to obtain Rhizoma Zingiberis Recens slurry with water according to the mass ratio of 1:20, be added thereto to The pectase of 0.006wt% and the cellulase of 0.004wt%, enzymolysis 30～60min at initial p H value 5.5,40 DEG C；Then Adding the glucoamylase of 0.012wt%, enzymolysis 30min～120min at original ph 4.5,55 DEG C, enzymolysis rises after completing Temperature, to 80 C insulations 6～10min, enzyme denaturing, cooling, obtains enzymolysis Rhizoma Zingiberis Recens slurry；
3) to step 2) gained enzymolysis Rhizoma Zingiberis Recens slurry in add 1～2wt% organic cellulose as filter aid agent, mix homogeneously, 100 mesh squeezing Filter, collect Rhizoma Zingiberis Recens liquid and Rhizoma Zingiberis Recens dregs respectively；
4) in the Rhizoma Zingiberis Recens liquid of step 3), add and described in 0.5～2wt% claim 1, be combined Sucus Zingberis clarifier, ultrasonic 5-60min, It is centrifuged 20～30 min through 5000 r/min, obtains clarification Sucus Zingberis；
5) step 3) gained Rhizoma Zingiberis Recens dregs 60 DEG C is dried, with the ethanol water of 60 wt % as extractant, and solid-liquid ratio 1:15g/mL, At ultrasonic power 120W, ultrasonic temperature 40 DEG C, extract 15min, filter, collect filtrate, rotated evaporate to obtain Rhizoma Zingiberis Recens extract；
6) in the clarification Sucus Zingberis of step 4) gained, add the Rhizoma Zingiberis Recens extract of 0.01～0.1 wt%, mix homogeneously, Rhizoma Zingiberis Recens must be clarified Juice extracting solution.
Preparation method the most according to claim 2, it is characterised in that: described, in step 1), Rhizoma Zingiberis Recens slice size is 0.5-1.0cm, thickness 0.2-0.3cm.
Preparation method the most according to claim 2, it is characterised in that: described, in step 1) colour fixative be pH4.5～ Aqueous citric acid solution, 0.02-0.10wt% ascorbic acid sodium water solution and the distannous citrate of 0.015-0.15wt% of 5.5 One or several mixing in aqueous solution.
Preparation method the most according to claim 2, it is characterised in that: described, twin-screw extruder in step 1) Jacket temperature be 40～45 DEG C, screw speed be 100～120r/min, die orifice aperture be 5～10mm.
Preparation method the most according to claim 2, it is characterised in that: described, in step 4), ultrasound condition is supersonic frequency Rate 0.1-1.0MHz, temperature 10-50 DEG C.
Preparation method the most according to claim 2, it is characterised in that: described, in step 4), compound Sucus Zingberis clarifier becomes It is divided into attapulgite clay loading chitosan, sodium alginate, sodium carboxymethyl cellulose, collagen and tea polyphenols.
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CN107183454A (en) * 2017-05-31 2017-09-22 佛山科学技术学院 A kind of root of kudzu vine vinegar beverage and preparation method thereof
CN107383930B (en) * 2017-06-15 2018-12-21 淮阴工学院 The method for extracting plant brown pigment
CN107383930A (en) * 2017-06-15 2017-11-24 淮阴工学院 The method for extracting plant brown pigment
CN107383115A (en) * 2017-06-15 2017-11-24 淮阴工学院 The method for extracting flower color glycosides
CN107312205A (en) * 2017-06-15 2017-11-03 淮阴工学院 The preparation and its application of recessed native loading chitosan cross linked ciclodextrines compound
CN107383115B (en) * 2017-06-15 2019-11-26 淮阴工学院 The method for extracting flower color glycosides
CN107624940A (en) * 2017-11-14 2018-01-26 安徽华健生物科技有限公司 A kind of processing method of honey ginger tea
CN110973429A (en) * 2019-12-20 2020-04-10 广西健美乐食品有限公司 Extraction method and application of bamboo juice
CN110973428A (en) * 2019-12-20 2020-04-10 广西健美乐食品有限公司 Danzhu liquid for clearing heat and reducing internal heat and preparation method thereof
CN111991514A (en) * 2020-08-18 2020-11-27 江西纳弗堂制药有限公司 Preparation method of rhizoma gastrodiae with ginger
CN112544929A (en) * 2020-12-09 2021-03-26 中华全国供销合作总社济南果品研究院 Method for producing clarified ginger juice by combination of step-by-step enzymolysis and high temperature
CN112869047A (en) * 2021-01-29 2021-06-01 河北天粮餐饮管理集团有限公司南京分公司 Special pork chop with good color and taste
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