CN105920667B - A kind of tissue engineering bracket and preparation method thereof - Google Patents
A kind of tissue engineering bracket and preparation method thereof Download PDFInfo
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- CN105920667B CN105920667B CN201610504003.8A CN201610504003A CN105920667B CN 105920667 B CN105920667 B CN 105920667B CN 201610504003 A CN201610504003 A CN 201610504003A CN 105920667 B CN105920667 B CN 105920667B
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
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Abstract
The present invention relates to a kind of tissue engineering brackets and preparation method thereof being made of mesh skeleton and cavernous body, belong to the field of medical instrument technology.The tissue engineering bracket is made of mesh skeleton and cavernous body, mesh skeleton material is the silk line or absorbable suture except sericin, mesh skeleton is the knitted structure of silk line or absorbable suture, cavernosa material be fibroin albumen with polyvinyl alcohol mass ratio be 1/9~1/1 it is compound, cavernous body is filled in the outside between the hole of mesh skeleton and being coated on mesh skeleton, tissue engineering bracket has uniform microstructure, good biocompatibility, provide the matrix structure of tissue cell growth, and meet the stability of tissue engineering bracket implantation human body initial stage mechanical property and the requirement of later period Lock-out, tissue engineering bracket preparation method of the present invention is simple, it is easy to operate, it is adapted to prepare the tissue engineering bracket of various different shapes and mechanical property requirements.
Description
Technical field
The present invention relates to the tissue engineering brackets and preparation method thereof of a kind of mesh skeleton and cavernous body composition, belong to medical treatment
Equipment technology field.
Background technique
Organizational project is the emerging interdisciplinary field to grow up in the late three decades, wherein most basic thinking is in body
In outer separation, culture cell inoculation to the bracket with certain space structure, by mutually sticking between cell, increasing is grown
It grows, extracellular matrix secretion, to form the tissue and organ with certain structure and function, the bracket of this certain space structure
It is referred to as tissue engineering bracket, such as skin, bone, cartilage, tendon, ligament.Need to meet four items as tissue engineering bracket
Part is suitble to biocompatible materials, the pore structure for providing cell growth and the cambium of cell tactophily to be adapted
Degradation characteristic, the structure and mechanical property for meeting tissue engineering bracket.Therefore, timbering material plays non-in Tissue Engineering Study
Normal important role, it is the key that organizational project realizes industrialization, and the processing method of timbering material occupies pole wherein
Its consequence.China Patent Publication No.: CN103157135A, publication date on June 19th, 2013, innovation and creation it is entitled
A kind of netted tissue-engineering stand is the silk to remove sericin this application discloses a kind of netted tissue-engineering stand
The net of braiding, composite collagen in mesh, hyaluronic acid, one of dredge aching and limp ossein or medical polyester-based polymer at chitosan
Synthetic material polylactic acid, polylactide-one of lactide, polyglycolic acid, polycaprolactone or a variety of.Its shortcoming is net
Compound material requires organic solvent to dissolve in hole, and culture, the breeding of these organic solvent cells are that have stronger toxicity,
With saying that solvent will remove after preparation, it can be difficult to completely removing, this is also present portion of tissue cell in tissue engineering bracket
When upper culture, the serious reason of later period cell death.It finds a kind of with material water-soluble and with good biocompatibility
It fills between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton and preparation method thereof, become this
The starting point of invention.
Summary of the invention
In view of the above problems, the invention aims to solve to lack existing for existing netted tissue-engineering stand
It falls into, is prepared using the aqueous-based dispersions of the compound water-soluble fibroin albumen of water-soluble polyvinyl alcohol or fibroin albumen powder multiple
Together in mandruka body in mesh skeleton, a kind of mechanical property for meeting tissue engineering bracket to bracket, uniform gap knot are provided
Structure and tissue engineering bracket and preparation method thereof entirely without toxicity requirements.
It realizes the technical scheme is that a kind of tissue engineering bracket, the tissue engineering bracket are by mesh skeleton
It is constituted with cavernous body, mesh skeleton material is the silk line or absorbable suture except sericin, and mesh skeleton is silk line
Or the knitted structure of absorbable suture, it is 1/9~1/1 to answer that cavernosa material, which is fibroin albumen with polyvinyl alcohol mass ratio,
It closes, cavernous body is filled in the outside between the hole of mesh skeleton and being coated on mesh skeleton.
One in braiding structure or twisted construction that the silk line is formed by 3~8 strands except the silk of sericin
Kind.
The absorbable suture is using one of absorbable plain catgut or chromicized catgut.
The fibroin albumen is the powder shaped fibroin albumen that the diameter that crushes is less than 500nm after natural silk degumming, or
After natural silk degumming, by dissolution, dialysis, the fibroin albumen being freeze-dried.
A kind of preparation method of tissue engineering bracket, the preparation including mesh skeleton, the fibroin albumen taken water as a solvent are molten
The preparation of liquid or fibroin albumen powder dispersion and poly-vinyl alcohol solution, the preparation method sequentially include the following steps:
A takes configured silk fibroin protein solution or fibroin albumen powder dispersion to mix and mixed with poly-vinyl alcohol solution
Solution is closed, wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 1/9~1/1, the total soluble matters matter of fibroin albumen and polyvinyl alcohol
Amount concentration is 5wt%~20wt%；
B is prepared corresponding with mould structure according to needing the shape of tissue engineering bracket to be prepared to select mold
Mesh skeleton is fixed in mold；
In the mold for being fixed with mesh skeleton that c again obtains mixed solution injection step b prepared by step a, freezing is solidifying
Gu demoulding, freeze-drying, obtain the prefabricated component of tissue engineering bracket；
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 2-4 hours, take out prefabricated component be heat-treated 15-30min in 120 DEG C of baking ovens, obtain cavernous body be filled in it is netted
Between the hole of skeleton and it is coated on the tissue engineering bracket outside mesh skeleton.
Due to using the technology described above, tissue engineering bracket of the present invention can effectively eliminate in existing tissue engineering bracket
Compound material requires organic solvent to dissolve in mesh, since organic solvent can not completely remove, remaining organic solvent
It is to have stronger toxicity to cell culture, breeding, causes the later death of cell culture, breeding serious.Group weaver of the invention
Engineering support, using the round tube or thin slice of silk line or the knitted structure of absorbable suture as the mesh skeleton of tissue engineering bracket,
Reticulated bone is filled in the aqueous-based dispersions of water-soluble polyvinyl alcohol, water-soluble fibroin albumen or fibroin albumen powder to prepare
Between the hole of frame and be coated on mesh skeleton outside cavernous body, be implemented without the tissue engineering bracket of organic solvent
Preparation, and make tissue engineering bracket that there is porous network structure, good mechanics and biocompatibility simultaneously.
Tissue engineering bracket of the invention, due to using the knitted structure mesh skeleton of silk line or absorbable suture,
It ensure that the excellent mechanical performances requirement of tissue engineering bracket；It can also be met by changing the silk number of share of stock of composition silk line
Requirement to the different mechanical properties of tissue engineering bracket；This tissue engineering bracket can be widely applied to tendon, ligament, cartilage,
The reparation and organizational project of the stretch-prooves tissues such as stomach wall, basin bottom also can be applied to repairing for the tissue of less demanding to stretch-proof
Multiple and organizational project.The slow degradation characteristic of silk line or absorbable suture ensure that tissue engineering bracket is implanted into human body simultaneously
Initial stage mechanical property stability and the Lock-out in later period requirement.
Mesh skeleton in tissue engineering bracket of the invention can be the knitted structure of silk line or absorbable suture
Round tube or thin slice.
The preparation method of tissue engineering bracket of the invention, since the mesh skeleton of tissue engineering bracket is knitted structure
Round tube or thin slice just facilitate and prepare one-dimensional, two-dimentional, three-dimensional and abnormally-structured mesh skeleton by the way of knitting；Reticulated bone
The technique by knitting that the mesh size of frame can be convenient is adjusted；It is by net simultaneously in tissue engineering bracket preparation process
Shape skeleton is fixed in mold the packing material for reinjecting mesh skeleton, can be prepared by selecting the planform of mold each
The tissue engineering bracket of kind different shape, structure.
Tissue engineering bracket of the invention, due to being compound silk fibroin protein solution or silk in the mesh skeleton of knitted structure
The mixture that fibroin powder dispersion is mixed with poly-vinyl alcohol solution obtains porous organizational project branch using freeze-drying
The prefabricated component of frame, it may be convenient to by concentration in change mixture come the size of adjustment apertures.Obtained organizational project branch
The prefabricated component of frame is handled using the vapour phase esterification of maleic anhydride, makes fibroin albumen or silk fibroin powder bluk recombination polyethylene
Alcohol does not dissolve in water, and the extension with the vapour phase esterification processing time can not only make fibroin albumen or silk fibroin powder bluk recombination polyethylene
Alcohol does not dissolve in the pore structure that water keeps stable, can further regulate and control fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol
Degradation speed.
The preparation method of tissue engineering bracket of the invention take fibroin albumen composite polyvinyl alcohol as the composite wood of filling
Material, polyvinyl alcohol is a kind of synthetic material of good biocompatibility.Natural material fibroin albumen further increases and ensure that group
The matrix condition that the biocompatibility of weaver's engineering support, especially fibroin powder remain natural biologic material cell development.Freeze
The pore structure of dry cavernous body provides space to internal stent breeding for cell；Fibroin albumen composite polyvinyl alcohol through esterification at
The slow growth characteristics of cambium are adapted on slow degradation characteristic and tissue engineering bracket after reason.Group weaver of the invention
Engineering support preparation method is simple and convenient to operate, is low in cost, has good controllability to preparation process, the present invention is adapted to
Prepare various different shapes, the tissue engineering bracket that different mechanical properties require.
Detailed description of the invention
Fig. 1 is tissue engineering bracket surface pattern electron microscope.
Specific embodiment
Present invention is further described in detail with reference to the accompanying drawings and examples.
See attached drawing.
A kind of tissue engineering bracket, the tissue engineering bracket are made of mesh skeleton and cavernous body, mesh skeleton material
Material is the silk line or absorbable suture except sericin, and mesh skeleton is the knitting knot of silk line or absorbable suture
Structure, cavernosa material be fibroin albumen with polyvinyl alcohol mass ratio be 1/9~1/1 it is compound, cavernous body is filled in mesh skeleton
Hole between and be coated on the outside of mesh skeleton.
One in braiding structure or twisted construction that the silk line is formed by 3~8 strands except the silk of sericin
Kind.
The absorbable suture is using one of absorbable plain catgut or chromicized catgut.
The fibroin albumen is the powder shaped fibroin albumen that the diameter that crushes is less than 500nm after natural silk degumming, or
After natural silk degumming, by dissolution, dialysis, the fibroin albumen being freeze-dried.
A kind of preparation method of tissue engineering bracket, the preparation including mesh skeleton, the fibroin albumen taken water as a solvent are molten
The preparation of liquid or fibroin albumen powder dispersion and poly-vinyl alcohol solution, the preparation method sequentially include the following steps:
A takes configured silk fibroin protein solution or fibroin albumen powder dispersion to mix and mixed with poly-vinyl alcohol solution
Solution is closed, wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 1/9~1/1, the total soluble matters matter of fibroin albumen and polyvinyl alcohol
Amount concentration is 5wt%~20wt%；
B is prepared corresponding with mould structure according to needing the shape of tissue engineering bracket to be prepared to select mold
Mesh skeleton is fixed in mold；
In the mold for being fixed with mesh skeleton that c again obtains mixed solution injection step b prepared by step a, freezing is solidifying
Gu demoulding, freeze-drying, obtain the prefabricated component of tissue engineering bracket；
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 2-4 hours, take out prefabricated component be heat-treated 15-30min in 120 DEG C of baking ovens, obtain cavernous body be filled in it is netted
Between the hole of skeleton and it is coated on the tissue engineering bracket outside mesh skeleton.
Silk fibroin protein solution or fibroin albumen powder dispersion in preparation step a as described above are warps after natural silk degumming
Dissolution is crossed, is dialysed, the fibroin albumen being freeze-dried is dispensed into water, and magnetic stirring is until be completely dissolved to obtain stable fibroin
Powder shaped fibroin albumen of the diameter crushed after protein solution or natural silk degumming less than 500nm is dispersed in water, ultrasound point
It dissipates and obtains the dispersion liquid of fibroin powder, the mass concentration of silk fibroin protein solution is 5wt%~20wt%；The fibroin albumen of dispersion liquid
The mass concentration of powder is 5wt%~20wt%；It is taken water as a solvent when preparing polyvinyl alcohol (PVA) solution, and the magnetic at 80 DEG C
Stirring.
Fibroin albumen powder can be prepared, silk for mulberry silk or after squeezing natural silk degumming by the method for mechanical crushing
The micro-structure of fibroin powder does not change with matrix feature；Fibroin albumen can pass through for mulberry silk or after squeezing natural silk degumming
Dissolution, dialysis, freeze-drying obtain.
Using one of absorbable plain catgut or chromicized catgut, the model of gutstring can be absorbable suture
Any one in 1#, 2#, 0#, 1/0,2/0,3/0,4/0,5/0.
Below with reference to embodiment, the invention will be further elaborated:
Embodiment one
The silk fibroin water solution that mass concentration is 5wt% by a, the polyvinyl alcohol water solution for being 5wt% with mass concentration
Be mixed to get mixed solution, wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 1/9, fibroin albumen and polyvinyl alcohol it is total molten
Matter mass concentration is 5wt%.
B select 3 strands of braiding structure lines except sericin silk prepare knitted structure tubular mesh skeleton and with circle
Cylindrical type mold of the same size is managed, and tubular mesh skeleton is fixed in cylindrical type mold；
C again infuses the mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is 5wt%
Enter that step b obtains is fixed in the mold of mesh skeleton, and cryocoagulation demoulds, and freeze-drying obtains tissue engineering bracket
Prefabricated component.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 2 hours, takes out prefabricated component and be heat-treated 15min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Between hole and it is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment two
The silk fibroin water solution that mass concentration is 8wt% by a, the polyvinyl alcohol water solution for being 10wt% with mass concentration
It is mixed into mixed solution, wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 3/7, the total soluble matters of fibroin albumen and polyvinyl alcohol
Mass concentration is 8wt%.
B selects 5 strands of slice-shaped mesh skeletons and thin slice that knitted structure is prepared except the twisted construction line of sericin silk
Sheet type mold of the same size, and slice-shaped mesh skeleton is fixed in sheet type mold.
C again infuses the mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is 8wt%
Enter that step b obtains is fixed in the mold of slice-shaped mesh skeleton, and cryocoagulation demoulds, and freeze-drying obtains organizational project
The prefabricated component of bracket.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 4 hours, takes out prefabricated component and be heat-treated 20min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Between hole and it is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment three
The silk fibroin water solution that mass concentration is 10wt% by a, it is water-soluble for the polyvinyl alcohol of 15wt% with mass concentration
Liquid is mixed into mixed solution, and wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 1/1, fibroin albumen and polyvinyl alcohol it is total molten
Matter mass concentration is 12wt%.
B selects 8 strands of slice-shaped mesh skeletons and thin slice that knitted structure is prepared except the braiding structure line of sericin silk
Sheet type mold of the same size, and slice-shaped mesh skeleton is fixed in sheet type mold.
The mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is again 12wt% by c
What injection step b was obtained is fixed in the mold of slice-shaped mesh skeleton, cryocoagulation, demoulds, and freeze-drying obtains a group weaver
The prefabricated component of engineering support.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 3 hours, takes out prefabricated component and be heat-treated 30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Between hole and it is coated on the tissue engineering bracket outside mesh skeleton.
Example IV
The silk fibroin water solution that mass concentration is 15wt% by a, it is water-soluble for the polyvinyl alcohol of 25wt% with mass concentration
Liquid is mixed into mixed solution, and wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 3/7, fibroin albumen and polyvinyl alcohol it is total molten
Matter mass concentration is 20wt%.
B selects absorbable plain catgut 1# to prepare the tubular mesh skeleton of knitted structure and of the same size with round tube
Cylindrical type mold, and tubular mesh skeleton is fixed in cylindrical type mold；
The mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is again 20wt% by c
What injection step b was obtained is fixed in the mold of mesh skeleton, cryocoagulation, demoulds, and freeze-drying obtains tissue engineering bracket
Prefabricated component.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 3.5 hours, takes out prefabricated component and be heat-treated 30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Hole between and be coated on the tissue engineering bracket outside mesh skeleton.
Embodiment five
The silk fibroin water solution that mass concentration is 12wt% by a, it is water-soluble for the polyvinyl alcohol of 25wt% with mass concentration
Liquid is mixed into mixed solution, and wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 4/7, fibroin albumen and polyvinyl alcohol it is total molten
Matter mass concentration is 15wt%.
B select absorbable chromicized catgut 2/0 prepare knitted structure slice-shaped mesh skeleton and thin slice it is of the same size
Sheet type mold, and slice-shaped mesh skeleton is fixed in sheet type mold.
The mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is again 15wt% by c
What injection step b was obtained is fixed in the mold of mesh skeleton, cryocoagulation, demoulds, and freeze-drying obtains tissue engineering bracket
Prefabricated component.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 3 hours, takes out prefabricated component and be heat-treated 30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Between hole and it is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment six
The silk fibroin water solution that mass concentration is 10wt% by a, it is water-soluble for the polyvinyl alcohol of 25wt% with mass concentration
Liquid is mixed into mixed solution, and wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 6/7, fibroin albumen and polyvinyl alcohol it is total molten
Matter mass concentration is 13wt%.
B select absorbable chromicized catgut 5/0 prepare knitted structure slice-shaped mesh skeleton and thin slice it is of the same size
Sheet type mold, and slice-shaped mesh skeleton is fixed in sheet type mold.
The mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is again 13wt% by c
What injection step b was obtained is fixed in the mold of mesh skeleton, cryocoagulation, demoulds, and freeze-drying obtains tissue engineering bracket
Prefabricated component.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 2.5 hours, takes out prefabricated component and be heat-treated 30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Hole between and be coated on the tissue engineering bracket outside mesh skeleton.
Embodiment seven
The silk fibroin water solution that mass concentration is 9wt% by a, the polyvinyl alcohol water solution for being 20wt% with mass concentration
It is mixed into mixed solution, wherein the mass ratio of fibroin albumen and polyvinyl alcohol is 5/7, the total soluble matters of fibroin albumen and polyvinyl alcohol
Mass concentration is 15wt%.
B selects absorbable plain catgut 3/0 to prepare the tubular mesh skeleton of knitted structure and in the same size with round tube
Cylindrical type mold, and tubular mesh skeleton is fixed in cylindrical type mold.
The mixed solution that the total soluble matters mass concentration of the step a fibroin albumen prepared and polyvinyl alcohol is again 15wt% by c
What injection step b was obtained is fixed in the mold of mesh skeleton, cryocoagulation, demoulds, and freeze-drying obtains tissue engineering bracket
Prefabricated component.
Step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere by d
After esterification and crosslinking 2 hours, takes out prefabricated component and be heat-treated 30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Between hole and it is coated on the tissue engineering bracket outside mesh skeleton.
Claims (1)
1. a kind of preparation method of tissue engineering bracket, the preparation including mesh skeleton, the fibroin albumen powder taken water as a solvent
The preparation of dispersion liquid and poly-vinyl alcohol solution, which is characterized in that the preparation method sequentially includes the following steps:
A takes fibroin albumen powder dispersion of the configured diameter less than 500nm to mix with poly-vinyl alcohol solution and is mixed
Solution, wherein the mass ratio of fibroin albumen powder and polyvinyl alcohol is 1/9~1/1, fibroin albumen powder and polyvinyl alcohol it is total
Solute mass concentration is 5wt%~20wt%；
B according to needing the shape of tissue engineering bracket to be prepared to select mold, by it is corresponding with mould structure prepare it is netted
Skeleton is fixed in mold；
In the mold for being fixed with mesh skeleton that c again obtains mixed solution injection step b prepared by step a, cryocoagulation takes off
Mould, freeze-drying, obtains the prefabricated component of tissue engineering bracket；
The step c prefabricated component for obtaining tissue engineering bracket is esterified by d in 80 DEG C, saturation maleic anhydride, HCl steam atmosphere
After crosslinking 2-4 hours, takes out prefabricated component and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain cavernous body and be filled in mesh skeleton
Hole between and be coated on the tissue engineering bracket outside mesh skeleton.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610504003.8A CN105920667B (en) | 2016-06-30 | 2016-06-30 | A kind of tissue engineering bracket and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610504003.8A CN105920667B (en) | 2016-06-30 | 2016-06-30 | A kind of tissue engineering bracket and preparation method thereof |
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| CN105920667A CN105920667A (en) | 2016-09-07 |
| CN105920667B true CN105920667B (en) | 2019-05-21 |
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| CN107982580A (en) * | 2017-12-08 | 2018-05-04 | 南通大学 | A kind of fibroin albumen tissue engineering bracket based on braiding and coating technology and its preparation method and application |
| CN109266608B (en) * | 2018-08-30 | 2021-12-10 | 宁波大学 | Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel |
| CN109865159A (en) * | 2018-12-20 | 2019-06-11 | 浙江星月生物科技股份有限公司 | A kind of silk network and its preparation method and application |
| CN111134747B (en) * | 2019-12-31 | 2020-12-18 | 南通纺织丝绸产业技术研究院 | Barb type silk suture line and preparation method thereof |
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| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN102188747A (en) * | 2010-03-04 | 2011-09-21 | 浙江大学 | Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof |
| CN102743796A (en) * | 2011-04-18 | 2012-10-24 | 北京汇亨创管理咨询有限公司 | Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof |
| CN103157135A (en) * | 2011-12-14 | 2013-06-19 | 西安瑞捷生物科技有限公司 | Reticular tissue engineering scaffold |
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| CN1895687A (en) * | 2006-06-09 | 2007-01-17 | 浙江大学 | Netted tissue-engineering stand |
| CN102188747A (en) * | 2010-03-04 | 2011-09-21 | 浙江大学 | Compound tissue engineering scaffold containing PLGA (poly(lactic-co-glycolic acid) strengthening net, and preparation method and application thereof |
| CN102743796A (en) * | 2011-04-18 | 2012-10-24 | 北京汇亨创管理咨询有限公司 | Silk fibroin porous support made from polyvinyl alcohol, and preparation method and application thereof |
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