CN105920667A - Scaffold for tissue engineering and preparation method thereof - Google Patents
Scaffold for tissue engineering and preparation method thereof Download PDFInfo
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- CN105920667A CN105920667A CN201610504003.8A CN201610504003A CN105920667A CN 105920667 A CN105920667 A CN 105920667A CN 201610504003 A CN201610504003 A CN 201610504003A CN 105920667 A CN105920667 A CN 105920667A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/16—Macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/10—Materials or treatment for tissue regeneration for reconstruction of tendons or ligaments
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- Oral & Maxillofacial Surgery (AREA)
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Abstract
The invention relates to a scaffold for tissue engineering composed of a meshed framework and a sponge body and a preparation method thereof, belonging to the technical field of medical apparatus and instruments. The scaffold for tissue engineering is composed of a meshed framework and a sponge body, wherein the meshed framework is made of silk threads without sericin or absorbable sutures and has a knitted structure of the silk threads or absorbable sutures; the sponge body is compounded of silk fibroin and polyvinyl alcohol according to a mass ratio of 1/9-1/1; the sponge body fills in pores of the meshed framework and coats the outer part of the meshed framework. The scaffold for tissue engineering has a uniform microstructure and good biocompatibility, provides a matrix structure of tissue cell growth and meets the requirements on stability of mechanical property and later natural disappearing in the initial stage of implanting the scaffold for tissue engineering into a human body. The preparation method of the scaffold for tissue engineering is simple and convenient to operate, and is suitable for preparing various scaffolds for tissue engineering of different shapes and mechanical properties.
Description
Technical field
The present invention relates to a kind of mesh skeleton tissue engineering bracket with spongy body composition and preparation method thereof, belong to
Technical field of medical instruments.
Background technology
Organizational project is the emerging interdisciplinary field grown up in the last thirty years, and the most most basic thinking is
Separate in vitro, cultivate cell and be inoculated on the support with certain space structure, mutual by between cell
Stick, growing multiplication, extracellular matrix secretion, thus formed and there is tissue and the organ of certain 26S Proteasome Structure and Function,
The support of this certain space structure is referred to as tissue engineering bracket, such as skin, bone, cartilage, tendon, ligament etc..
Need to meet four conditions as tissue engineering bracket, be suitable for the biocompatible materials of cell tactophily, carry
Supply the degradation characteristic that the pore structure of cell growth adapts, the knot meeting tissue engineering bracket with cambium
Structure and mechanical property.Therefore, timbering material plays very important effect in Tissue Engineering Study, and it is group
Knit the key of Project Realization industrialization, and the processing method of timbering material is occupied extremely importantly wherein
Position.China Patent Publication No.: CN103157135A, publication date on June 19th, 2013, the name of innovation and creation
Being referred to as a kind of netted tissue-engineering stand, this application case discloses a kind of netted tissue-engineering stand for remove silk
The net of the silkworm silk braiding of glue protein, in mesh in composite collagen, chitosan, hyaluronic acid, thin aching and limp ossein
One, the lactide of or medical polyesters high molecular synthetic material polylactic acid, polylactide-, polyglycolic acid, poly-
One or more in caprolactone.It is molten that the material that its weak point is compound in mesh is required for organic solvent
Solving, the cultivation of these organic solvent cells, breeding are to have stronger toxicity, with saying that solvent to be removed after the production,
It can be difficult to completely remove, this is also present portion of tissue cell when cultivating on tissue engineering bracket, and the later stage is thin
The dead serious reason of born of the same parents.Find a kind of with water miscible and there is the material of good biocompatibility fill in net
Between the hole of shape skeleton and be coated on tissue engineering bracket outside mesh skeleton and preparation method thereof, become this
The starting point of invention.
Summary of the invention
For the problem of above-mentioned existence, the invention aims to solve what existing netted tissue-engineering stand existed
Defect, uses water miscible polyvinyl alcohol to be combined water miscible fibroin albumen or the aqueous dispersion of fibroin albumen powder body
Liquid is prepared and is compound in mandruka body in mesh skeleton, it is provided that a kind of meet the tissue engineering bracket power to support
Learn performance, uniform pore structure and the tissue engineering bracket and preparation method thereof entirely without toxicity requirements.
Realization the technical scheme is that a kind of tissue engineering bracket, and described tissue engineering bracket is by netted
Skeleton is constituted with spongy body, and mesh skeleton material is the silkworm silk line except sericin or absorbable suture, netted
Skeleton is the knitted structure of silkworm silk line or absorbable suture, and cavernosa material is fibroin albumen and polyvinyl alcohol matter
Amount than be 1/9～1/1 compound, spongy body is filled between the hole of mesh skeleton and is coated on mesh skeleton
Outside.
Described silkworm silk line is by 3～8 strands of braiding structures formed except the silkworm silk of sericin or twisted construction
One.
Described absorbable suture uses the one in absorbable plain catgut or chromicized catgut.
Described fibroin albumen is the powder shaped fibroin egg pulverizing the diameter obtained after natural silk degumming less than 500nm
In vain, or after natural silk degumming, through dissolving, dialysis, the fibroin albumen that lyophilization obtains.
The preparation method of a kind of tissue engineering bracket, including the preparation of mesh skeleton, the fibroin egg with water as solvent
White solution or fibroin albumen powder dispersion and the preparation of poly-vinyl alcohol solution, described preparation method is according to the following steps
Carry out:
A takes the silk fibroin protein solution configured or fibroin albumen powder dispersion mixes mutually with poly-vinyl alcohol solution
Obtaining mixed solution, wherein fibroin albumen is 1/9～1/1 with the mass ratio of polyvinyl alcohol, and fibroin albumen is with poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%～20wt%；
B selects mould, by the preparation corresponding with mould structure according to the shape of the tissue engineering bracket needing preparation
Good mesh skeleton is fixed in mould；
In the mould being fixed with mesh skeleton that the mixed solution implantation step b that step a is prepared by c again obtains,
Cryocoagulation, the demoulding, lyophilization, obtain the prefabricated component of tissue engineering bracket；
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2-4 hour, takes out prefabricated component heat treatment 15-30min in 120 DEG C of baking ovens,
Obtain spongy body be filled between the hole of mesh skeleton and be coated on the tissue engineering bracket outside mesh skeleton.
Owing to using above technical scheme, tissue engineering bracket of the present invention can effectively eliminate existing organizational project and prop up
Material compound in mesh in frame is required for organic solvent and dissolves, owing to organic solvent cannot completely remove, residual
Cell is cultivated by the organic solvent deposited, breeding is to have stronger toxicity, and the later stage cause cell to cultivate, breeding is dead
Die serious.The tissue engineering bracket of the present invention, with silkworm silk line or the pipe of the knitted structure of absorbable suture or
Thin slice is the mesh skeleton of tissue engineering bracket, with water miscible polyvinyl alcohol, water-soluble fibroin albumen or fibroin
The aqueous-based dispersions of protein powder is prepared between the hole being filled in mesh skeleton and is coated on mesh skeleton
Outside spongy body, it is achieved need not the preparation of the tissue engineering bracket of organic solvent, and make organizational project simultaneously
Support has porous network structure, good mechanics and biocompatibility.
The tissue engineering bracket of the present invention, owing to using silkworm silk line or the knitted structure reticulated bone of absorbable suture
Frame, it is ensured that the excellent mechanical performances requirement of tissue engineering bracket；Can also be by changing the silkworm of composition silkworm silk line
Thread number meets the requirement of the different mechanical properties to tissue engineering bracket；This tissue engineering bracket can extensively be answered
For the reparation of stretch-proof tissue such as at the bottom of tendon, ligament, cartilage, stomach wall, basin and organizational project, it is also possible to should
Reparation and organizational project for tissue less demanding to stretch-proof.Silkworm silk line or absorbable suture simultaneously
Slowly degradation characteristic ensure that tissue engineering bracket implants stability and the later stage of the mechanical property at the initial stage of human body
The requirement of Lock-out.
Mesh skeleton in the tissue engineering bracket of the present invention can be the knitting of silkworm silk line or absorbable suture
The pipe of structure or thin slice.
The preparation method of the tissue engineering bracket of the present invention, owing to the mesh skeleton of tissue engineering bracket is knitting knot
The pipe of structure or thin slice, the knitting mode of the most convenient employing prepares one-dimensional, two-dimentional, three-dimensional and abnormally-structured net
Shape skeleton；The mesh size of mesh skeleton can be regulated by knitting technique easily；Organizational project simultaneously
In support preparation process, be mesh skeleton is fixed on mould in reinject the packing material of mesh skeleton, permissible
The tissue engineering bracket of various difformity, structure is prepared by the planform selecting mould.
The tissue engineering bracket of the present invention, owing to being compound silk fibroin protein solution in the mesh skeleton of knitted structure
Or the mixture that fibroin albumen powder dispersion mixes with poly-vinyl alcohol solution, then obtain porous through lyophilization
The prefabricated component of tissue engineering bracket, in that context it may be convenient to carry out the big of adjustment apertures by changing concentration in mixture
Little.The prefabricated component of obtained tissue engineering bracket processes through the vapour phase esterification of maleic anhydride again, makes silk
Fibroin or silk fibroin powder bluk recombination polyvinyl alcohol do not dissolve in water, process the prolongation of time not only with vapour phase esterification
Fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol can be made not to dissolve in the pore structure that water keeps stable, can
Regulation and control fibroin albumen or the degradation speed of silk fibroin powder bluk recombination polyvinyl alcohol further.
The preparation method of the tissue engineering bracket of the present invention, compound with fibroin albumen composite polyvinyl alcohol for fill
Material, polyvinyl alcohol is the synthetic material of a kind of good biocompatibility.Natural material fibroin albumen improves further,
And ensure that the biocompatibility of tissue engineering bracket, particularly fibroin powder body remains natural biologic material cell
The matrix condition grown.The pore structure of lyophilizing spongy body is that cell provides space to internal stent breeding；Silk
The fibroin composite polyvinyl alcohol slow degradation characteristic after esterification treatment and cambium on tissue engineering bracket
Slow growth characteristics adapt.The tissue engineering bracket preparation method of the present invention is simple and convenient to operate, cost
Cheap, preparation process is had good controllability, the present invention is adapted to prepare various difformity, different power
Learn the tissue engineering bracket of performance requirement.
Accompanying drawing explanation
Fig. 1 is tissue engineering bracket surface topography Electronic Speculum figure.
Detailed description of the invention
With embodiment, the present invention is described in further detail below in conjunction with the accompanying drawings.
See accompanying drawing.
A kind of tissue engineering bracket, described tissue engineering bracket is to be made up of with spongy body mesh skeleton, reticulated bone
Frame material is the silkworm silk line except sericin or absorbable suture, and mesh skeleton is silkworm silk line or absorbable stitching
The knitted structure of line, cavernosa material be fibroin albumen with polyvinyl alcohol mass ratio be 1/9～1/1 compound,
Spongy body is filled between the hole of mesh skeleton and is coated on the outside of mesh skeleton.
Described silkworm silk line is by 3～8 strands of braiding structures formed except the silkworm silk of sericin or twisted construction
One.
Described absorbable suture uses the one in absorbable plain catgut or chromicized catgut.
Described fibroin albumen is the powder shaped fibroin egg pulverizing the diameter obtained after natural silk degumming less than 500nm
In vain, or after natural silk degumming, through dissolving, dialysis, the fibroin albumen that lyophilization obtains.
The preparation method of a kind of tissue engineering bracket, including the preparation of mesh skeleton, the fibroin egg with water as solvent
White solution or fibroin albumen powder dispersion and the preparation of poly-vinyl alcohol solution, described preparation method is according to the following steps
Carry out:
A takes the silk fibroin protein solution configured or fibroin albumen powder dispersion mixes mutually with poly-vinyl alcohol solution
Obtaining mixed solution, wherein fibroin albumen is 1/9～1/1 with the mass ratio of polyvinyl alcohol, and fibroin albumen is with poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%～20wt%；
B selects mould, by the preparation corresponding with mould structure according to the shape of the tissue engineering bracket needing preparation
Good mesh skeleton is fixed in mould；
In the mould being fixed with mesh skeleton that the mixed solution implantation step b that step a is prepared by c again obtains,
Cryocoagulation, the demoulding, lyophilization, obtain the prefabricated component of tissue engineering bracket；
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2-4 hour, takes out prefabricated component heat treatment 15-30min in 120 DEG C of baking ovens,
Obtain spongy body be filled between the hole of mesh skeleton and be coated on the tissue engineering bracket outside mesh skeleton.
Silk fibroin protein solution or fibroin albumen powder dispersion in preparation process a as above, be natural silk degumming
After, through dissolving, dialysis, the fibroin albumen that lyophilization obtains is dispensed in water, and magnetic stirring is until the most molten
Solution obtains stable silk fibroin protein solution, or pulverizes the diameter the obtained powder shaped less than 500nm after natural silk degumming
Fibroin albumen is dispersed in water, and ultrasonic disperse obtains the dispersion liquid of fibroin powder body, and the quality of silk fibroin protein solution is dense
Degree is 5wt%～20wt%；The mass concentration of the fibroin albumen powder body of dispersion liquid is 5wt%～20wt%；Join
With water as solvent during polyvinyl alcohol processed (PVA) solution, and magnetic stirring at 80 DEG C.
After fibroin albumen powder body can be mulberry silk or squeezing natural silk degumming, it is prepared into by the method for mechanical activation comminution
Arriving, the micro structure of fibroin albumen powder body does not change with substrate feature；Fibroin albumen can be mulberry silk or squeeze silkworm
After degumming of silk, through dissolving, dialysis, lyophilization obtains.
Absorbable suture uses the one in absorbable plain catgut or chromicized catgut, and the model of gutstring is permissible
It is any one in 1#, 2#, 0#, 1/0,2/0,3/0,4/0,5/0.
Below in conjunction with embodiment, the invention will be further elaborated:
Embodiment one
Mass concentration is the silk fibroin water solution of 5wt% by a, is the polyvinyl alcohol of 5wt% with mass concentration
Aqueous solution obtains mixed solution, and wherein fibroin albumen is 1/9 with the mass ratio of polyvinyl alcohol, fibroin albumen
It is 5wt% with the total soluble matters mass concentration of polyvinyl alcohol.
B selects 3 strands of tubular mesh skeletons preparing knitted structure except the braiding structure line of sericin silkworm silk
And column type mould of the same size with pipe, and tubular mesh skeleton is fixed in column type mould；
The fibroin albumen that step a is prepared by c again and the mixing that total soluble matters mass concentration is 5wt% of polyvinyl alcohol
In the mould being fixed with mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, lyophilization,
Obtain the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2 hours, takes out prefabricated component heat treatment 15min in 120 DEG C of baking ovens, obtains
Spongy body is filled between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment two
Mass concentration is the silk fibroin water solution of 8wt% by a, is the polyvinyl alcohol of 10wt% with mass concentration
Aqueous solution is to mixed solution, and wherein fibroin albumen is 3/7 with the mass ratio of polyvinyl alcohol, fibroin albumen with
The total soluble matters mass concentration of polyvinyl alcohol is 8wt%.
B selects 5 strands of slice-shaped mesh skeletons preparing knitted structure except the twisted construction line of sericin silkworm silk
Sheet type mould of the same size with thin slice, and slice-shaped mesh skeleton is fixed in sheet type mould.
The fibroin albumen that step a is prepared by c again and the mixing that total soluble matters mass concentration is 8wt% of polyvinyl alcohol
What solution-injecting step b obtained is fixed with in the mould of slice-shaped mesh skeleton, cryocoagulation, the demoulding, freezing
It is dried, obtains the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 4 hours, takes out prefabricated component heat treatment 20min in 120 DEG C of baking ovens, obtains
Spongy body is filled between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment three
Mass concentration is the silk fibroin water solution of 10wt% by a, is the polyethylene of 15wt% with mass concentration
Alcohol-water solution is mixed into mixed solution, and wherein fibroin albumen is 1/1 with the mass ratio of polyvinyl alcohol, fibroin albumen
It is 12wt% with the total soluble matters mass concentration of polyvinyl alcohol.
B selects 8 strands of slice-shaped mesh skeletons preparing knitted structure except the braiding structure line of sericin silkworm silk
Sheet type mould of the same size with thin slice, and slice-shaped mesh skeleton is fixed in sheet type mould.
The fibroin albumen that step a is prepared by c again and the total soluble matters mass concentration of polyvinyl alcohol are the mixed of 12wt%
Close in the mould being fixed with slice-shaped mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, cold
Lyophilizing is dry, obtains the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 3 hours, takes out prefabricated component heat treatment 30min in 120 DEG C of baking ovens, obtains
Spongy body is filled between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment four
Mass concentration is the silk fibroin water solution of 15wt% by a, is the polyethylene of 25wt% with mass concentration
Alcohol-water solution is mixed into mixed solution, and wherein fibroin albumen is 3/7 with the mass ratio of polyvinyl alcohol, fibroin albumen
It is 20wt% with the total soluble matters mass concentration of polyvinyl alcohol.
B select absorbable plain catgut 1# prepare knitted structure tubular mesh skeleton and with pipe size
Consistent column type mould, and tubular mesh skeleton is fixed in column type mould；
The fibroin albumen that step a is prepared by c again and the total soluble matters mass concentration of polyvinyl alcohol are the mixed of 20wt%
Close in the mould being fixed with mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, lyophilization,
Obtain the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 3.5 hours, takes out prefabricated component heat treatment 30min in 120 DEG C of baking ovens,
It is filled in spongy body between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment five
Mass concentration is the silk fibroin water solution of 12wt% by a, is the polyethylene of 25wt% with mass concentration
Alcohol-water solution is mixed into mixed solution, and wherein fibroin albumen is 4/7 with the mass ratio of polyvinyl alcohol, fibroin albumen
It is 15wt% with the total soluble matters mass concentration of polyvinyl alcohol.
B selects absorbable chromicized catgut 2/0 to prepare slice-shaped mesh skeleton and the thin slice size of knitted structure
Consistent sheet type mould, and slice-shaped mesh skeleton is fixed in sheet type mould.
The fibroin albumen that step a is prepared by c again and the total soluble matters mass concentration of polyvinyl alcohol are the mixed of 15wt%
Close in the mould being fixed with mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, lyophilization,
Obtain the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 3 hours, takes out prefabricated component heat treatment 30min in 120 DEG C of baking ovens, obtains
Spongy body is filled between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment six
Mass concentration is the silk fibroin water solution of 10wt% by a, is the polyethylene of 25wt% with mass concentration
Alcohol-water solution is mixed into mixed solution, and wherein fibroin albumen is 6/7 with the mass ratio of polyvinyl alcohol, fibroin albumen
It is 13wt% with the total soluble matters mass concentration of polyvinyl alcohol.
B selects absorbable chromicized catgut 5/0 to prepare slice-shaped mesh skeleton and the thin slice size of knitted structure
Consistent sheet type mould, and slice-shaped mesh skeleton is fixed in sheet type mould.
The fibroin albumen that step a is prepared by c again and the total soluble matters mass concentration of polyvinyl alcohol are the mixed of 13wt%
Close in the mould being fixed with mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, lyophilization,
Obtain the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2.5 hours, takes out prefabricated component heat treatment 30min in 120 DEG C of baking ovens,
It is filled in spongy body between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Embodiment seven
Mass concentration is the silk fibroin water solution of 9wt% by a, is the polyvinyl alcohol of 20wt% with mass concentration
Aqueous solution is to mixed solution, and wherein fibroin albumen is 5/7 with the mass ratio of polyvinyl alcohol, fibroin albumen with
The total soluble matters mass concentration of polyvinyl alcohol is 15wt%.
B selects absorbable plain catgut 3/0 to prepare the tubular mesh skeleton of knitted structure and big with pipe
Little consistent column type mould, and tubular mesh skeleton is fixed in column type mould.
The fibroin albumen that step a is prepared by c again and the total soluble matters mass concentration of polyvinyl alcohol are the mixed of 15wt%
Close in the mould being fixed with mesh skeleton that solution-injecting step b obtains, cryocoagulation, the demoulding, lyophilization,
Obtain the prefabricated component of tissue engineering bracket.
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2 hours, takes out prefabricated component heat treatment 30min in 120 DEG C of baking ovens, obtains
Spongy body is filled between the hole of mesh skeleton and is coated on the tissue engineering bracket outside mesh skeleton.
Claims (5)
1. a tissue engineering bracket, it is characterised in that: described tissue engineering bracket is by mesh skeleton and sponge
Body is constituted, and mesh skeleton material is the silkworm silk line except sericin or absorbable suture, and mesh skeleton is silkworm silk
Line or the knitted structure of absorbable suture, cavernosa material is that fibroin albumen is with polyvinyl alcohol mass ratio
1/9～1/1 compound, spongy body is filled between the hole of mesh skeleton and is coated on the outside of mesh skeleton.
2. tissue engineering bracket as claimed in claim 1 a kind of, it is characterised in that: described silkworm silk line be by
One in 3～8 strands of braiding structures except the silkworm silk composition of sericin or twisted construction.
3. a kind of tissue engineering bracket as claimed in claim 1, it is characterised in that: described absorbable stitching
Line uses the one in absorbable plain catgut or chromicized catgut.
4. a kind of tissue engineering bracket as claimed in claim 1, it is characterised in that: described fibroin albumen is
Pulverize the diameter the obtained powder shaped fibroin albumen less than 500nm after natural silk degumming, or after natural silk degumming, pass through
Dissolve, dialysis, the fibroin albumen that lyophilization obtains.
5. a preparation method for tissue engineering bracket, including the preparation of mesh skeleton, the fibroin with water as solvent
Protein solution or fibroin albumen powder dispersion and the preparation of poly-vinyl alcohol solution, it is characterised in that described preparation
Method sequentially includes the following steps:
A takes the silk fibroin protein solution configured or fibroin albumen powder dispersion mixes mutually with poly-vinyl alcohol solution
Obtaining mixed solution, wherein fibroin albumen is 1/9～1/1 with the mass ratio of polyvinyl alcohol, and fibroin albumen is with poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%～20wt%；
B selects mould, by the preparation corresponding with mould structure according to the shape of the tissue engineering bracket needing preparation
Good mesh skeleton is fixed in mould；
In the mould being fixed with mesh skeleton that the mixed solution implantation step b that step a is prepared by c again obtains,
Cryocoagulation, the demoulding, lyophilization, obtain the prefabricated component of tissue engineering bracket；
D step c is obtained the prefabricated component of tissue engineering bracket in 80 DEG C, saturated maleic anhydride, HCl
In steam atmosphere, esterification and crosslinking is after 2-4 hour, takes out prefabricated component heat treatment 15-30min in 120 DEG C of baking ovens,
Obtain spongy body be filled between the hole of mesh skeleton and be coated on the tissue engineering bracket outside mesh skeleton.
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| CN106474543A (en) * | 2016-09-22 | 2017-03-08 | 武汉纺织大学 | A kind of enhancement mode ligament tissue engineering rack and preparation method thereof |
| CN107982580A (en) * | 2017-12-08 | 2018-05-04 | 南通大学 | A kind of fibroin albumen tissue engineering bracket based on braiding and coating technology and its preparation method and application |
| CN109266608A (en) * | 2018-08-30 | 2019-01-25 | 宁波大学 | Utilize polyvinyl alcohol/silk fibroin hydrogel regulation nerve growth method |
| CN109865159A (en) * | 2018-12-20 | 2019-06-11 | 浙江星月生物科技股份有限公司 | A kind of silk network and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109266608A (en) * | 2018-08-30 | 2019-01-25 | 宁波大学 | Utilize polyvinyl alcohol/silk fibroin hydrogel regulation nerve growth method |
| CN109266608B (en) * | 2018-08-30 | 2021-12-10 | 宁波大学 | Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel |
| CN109865159A (en) * | 2018-12-20 | 2019-06-11 | 浙江星月生物科技股份有限公司 | A kind of silk network and its preparation method and application |
| CN111134747A (en) * | 2019-12-31 | 2020-05-12 | 南通纺织丝绸产业技术研究院 | Barb type silk suture line and preparation method thereof |
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