CN109266608B - Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel - Google Patents
Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel Download PDFInfo
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- CN109266608B CN109266608B CN201810999527.8A CN201810999527A CN109266608B CN 109266608 B CN109266608 B CN 109266608B CN 201810999527 A CN201810999527 A CN 201810999527A CN 109266608 B CN109266608 B CN 109266608B
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- polyvinyl alcohol
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- 239000004372 Polyvinyl alcohol Substances 0.000 title claims abstract description 75
- 229920002451 polyvinyl alcohol Polymers 0.000 title claims abstract description 75
- 239000000017 hydrogel Substances 0.000 title claims abstract description 50
- 108010022355 Fibroins Proteins 0.000 title claims abstract description 39
- 210000002569 neuron Anatomy 0.000 title claims abstract description 39
- 230000012010 growth Effects 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 20
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 15
- 230000001276 controlling effect Effects 0.000 title claims abstract description 10
- 238000007710 freezing Methods 0.000 claims description 12
- 230000008014 freezing Effects 0.000 claims description 12
- 210000004027 cell Anatomy 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 210000000274 microglia Anatomy 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 6
- 208000005890 Neuroma Diseases 0.000 claims description 6
- 238000010257 thawing Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 210000003855 cell nucleus Anatomy 0.000 claims description 3
- 210000000805 cytoplasm Anatomy 0.000 claims description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 claims description 2
- 206010029260 Neuroblastoma Diseases 0.000 claims description 2
- 210000001130 astrocyte Anatomy 0.000 claims description 2
- 210000001185 bone marrow Anatomy 0.000 claims description 2
- 239000012091 fetal bovine serum Substances 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 210000004295 hippocampal neuron Anatomy 0.000 claims description 2
- 239000002609 medium Substances 0.000 claims description 2
- 230000035755 proliferation Effects 0.000 claims description 2
- 210000001178 neural stem cell Anatomy 0.000 claims 1
- 239000003102 growth factor Substances 0.000 abstract description 3
- 238000004113 cell culture Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000003969 blast cell Anatomy 0.000 description 3
- 210000000988 bone and bone Anatomy 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000005709 nerve cell growth Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 108010069514 Cyclic Peptides Proteins 0.000 description 1
- 102000001189 Cyclic Peptides Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 102000009123 Fibrin Human genes 0.000 description 1
- 108010073385 Fibrin Proteins 0.000 description 1
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108010009711 Phalloidine Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229950003499 fibrin Drugs 0.000 description 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 1
- 238000003760 magnetic stirring Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2329/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal, or ketal radical; Hydrolysed polymers of esters of unsaturated alcohols with saturated carboxylic acids; Derivatives of such polymer
- C08J2329/02—Homopolymers or copolymers of unsaturated alcohols
- C08J2329/04—Polyvinyl alcohol; Partially hydrolysed homopolymers or copolymers of esters of unsaturated alcohols with saturated carboxylic acids
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2489/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
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- Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Dispersion Chemistry (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Oncology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
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Abstract
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Priority Applications (1)
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CN201810999527.8A CN109266608B (en) | 2018-08-30 | 2018-08-30 | Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel |
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CN201810999527.8A CN109266608B (en) | 2018-08-30 | 2018-08-30 | Method for regulating and controlling growth of nerve cells by using polyvinyl alcohol/silk fibroin hydrogel |
Publications (2)
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CN109266608A CN109266608A (en) | 2019-01-25 |
CN109266608B true CN109266608B (en) | 2021-12-10 |
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Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114249907B (en) * | 2021-12-31 | 2023-03-28 | 西南大学 | Preparation method of luminous and stretchable silk fibroin/polyvinyl alcohol hydrogel |
CN114854052A (en) * | 2022-07-06 | 2022-08-05 | 苏州大学 | Self-repairing and self-adhesive silk fibroin anti-freezing conductive hydrogel and preparation method and application thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101028536A (en) * | 2007-03-02 | 2007-09-05 | 上海交通大学 | Production of polyvinyl alcohol/sericin blended gel thin film |
CN101603007A (en) * | 2009-07-21 | 2009-12-16 | 重庆理工大学 | A kind of method for preparing cell culture vector |
US8048989B2 (en) * | 2002-06-19 | 2011-11-01 | National Institute Of Agrobiological Sciences | Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers |
CN105920667A (en) * | 2016-06-30 | 2016-09-07 | 武汉纺织大学 | Scaffold for tissue engineering and preparation method thereof |
CN106701656A (en) * | 2015-07-27 | 2017-05-24 | 天津卫凯生物工程有限公司 | Composite scaffold for cell culture and preparation method thereof |
-
2018
- 2018-08-30 CN CN201810999527.8A patent/CN109266608B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8048989B2 (en) * | 2002-06-19 | 2011-11-01 | National Institute Of Agrobiological Sciences | Biodegradable biopolymers, method for their preparation and functional materials constituted by these biopolymers |
CN101028536A (en) * | 2007-03-02 | 2007-09-05 | 上海交通大学 | Production of polyvinyl alcohol/sericin blended gel thin film |
CN101603007A (en) * | 2009-07-21 | 2009-12-16 | 重庆理工大学 | A kind of method for preparing cell culture vector |
CN106701656A (en) * | 2015-07-27 | 2017-05-24 | 天津卫凯生物工程有限公司 | Composite scaffold for cell culture and preparation method thereof |
CN105920667A (en) * | 2016-06-30 | 2016-09-07 | 武汉纺织大学 | Scaffold for tissue engineering and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
PVA-Silk 复合水凝胶的启动摩擦性能研究;张德坤等;《中国矿业大学学报》;20081130;第37卷(第6期);全文 * |
镶嵌纳米级再生丝素蛋白聚乙烯醇膜生物材料的制备及其应用研究;郝雪菲;《中国优秀硕士学位论文全文数据库 医药卫生科技辑》;20110515;第31页第1段,第37页 * |
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Effective date of registration: 20240527 Address after: Room 3205, Building 2, Dongfangzhimen Building, No. 199 Xinggang Street, Industrial Park, Suzhou City, Jiangsu Province, 215000 Patentee after: Suzhou Hanhai Boyuan Intellectual Property Services Co.,Ltd. Country or region after: China Address before: 315211, Fenghua Road, Jiangbei District, Zhejiang, Ningbo 818 Patentee before: Ningbo University Country or region before: China |
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Effective date of registration: 20240605 Address after: 519000 Card slot C12, Room 301, Research Headquarters Building, Guangdong Macao Cooperation Traditional Chinese Medicine Technology Industrial Park, No.1 Doukou Road, Hengqin New District, Zhuhai City, Guangdong Province Patentee after: Guangdong Hengqin Guangdong Macao Deep Cooperation Zone Qimei International Stem Cell Hospital Co.,Ltd. Country or region after: China Patentee after: Guangdong Qimei Life Medicine Technology Research Institute Patentee after: Guangdong Stanfu International Stem Cell Medical Research Institute Patentee after: Guangdong Qimei Pharmaceutical Biotechnology Group Co.,Ltd. Patentee after: Zhuhai Qimei Stem Cell Bank Co.,Ltd. Patentee after: Beijing Qimei Xinghua Gene Medical Technology Center Address before: Room 3205, Building 2, Dongfangzhimen Building, No. 199 Xinggang Street, Industrial Park, Suzhou City, Jiangsu Province, 215000 Patentee before: Suzhou Hanhai Boyuan Intellectual Property Services Co.,Ltd. Country or region before: China |
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