CN105920344A - Traditional Chinese medicine enema liquid for treating damp-heat ulcerative colitis - Google Patents

Abstract

The invention relates to a traditional Chinese medicine enema liquid for treating damp-heat ulcerative colitis. The traditional Chinese medicine enema liquid can effectively solve the problem of medication of ulcerative colitis. The technical scheme includes that the traditional Chinese medicine enema liquid is prepared from raw drugs including, by weight, 20-30g of Chinese pulsatilla roots, 15-25g of pomegranate peel, 20-30g of bletilla striata, 15-25g of Chinese gall, 12-20g of tribulus terrestris, 15-20g of coptis chinensis and 15-20g of radix sophorae flavescentis. The traditional Chinese medicine enema liquid has fine clinical effects for treating damp-heat ulcerative colitis and has the advantages that the enema liquid is scientific in formula, reasonable in compatibility, rich in medicinal source, simple in preparation process, easy to carry, convenient to use, low in cost, better in effect, free of side effects and high in safety, and the enema liquid can effectively treat clinical symptoms of the ulcerative colitis and is an innovated drug for treating the ulcerative colitis.

Description

A kind of Chinese medicinal herbs decoction treating retention of damp-heat in the interior type ulcerative colitis

Technical field

The present invention relates to field of medicaments, a kind of Chinese medicinal herbs decoction treating retention of damp-heat in the interior type ulcerative colitis.

Background technology

First ulcerative colitis pathological changes is from the beginning of the diffusivity inflammatory disorders of shallow-layer mucosa, edema followed by occurs, fills Blood, plumpness and tissue fragility increase, and produce point-like aphtha, and then develop into big ulcer, and late period is due to colon's hypertrophy, intestinal Wall is thickening, enteric cavity narrows, and intestinal tube shortens, and structure tends to occur ANOMALOUS VARIATIONS.After clinic mainly shows as diarrhoea, stomachache and pain in the abdomen Weight, mucopurulent bloody stool.State of an illness weight, many recurrent exerbation or long-term protracted course of disease, the later stage is the Chronic Progressive course of disease, the state of an illness Seriously accompany complications, as narrow in intestinal tube, intestinal fistula, block, adhesion, canceration etc., excision pathological tissues can only be taked, give and suffer from Person's body and mind brings the biggest wound.In the last few years along with environmental change, the multiformity of dietary structure, the increasing of life stress, from The many factors such as body immunity reduction cause the sickness rate of ulcerative colitis significantly raised, and the state of an illness is in increasing the weight of trend.Therefore, control The improvement treated on the medicine of ulcerative colitis is the problem needing solution at present badly.

Summary of the invention

For above-mentioned situation, for solving the defect of prior art, the purpose of the present invention is just to provide one and treats damp and hot interior Accumulate the Chinese medicinal herbs decoction of type ulcerative colitis, can effectively solve the medication problem of ulcerative colitis, hence it is evident that improve clinic and treat Effect.

The technical scheme that the present invention solves is that this Chinese medicinal herbs decoction is made up of the material medicine of following weight meter: hoary hair Father-in-law 20-30g, Pericarpium Granati 15-25g, Pseudobulbus Bletillae (Rhizoma Bletillae) 20-30g, Galla Chinensis 15-25g, Fructus Atriplicis Sibiricae 12-20g, Rhizoma Coptidis 15-20g and Radix Sophorae Flavescentis 15-20g, adds water to submerge by above-mentioned raw materials medicament mixed together, soaks 20～30min, and slow fire simmers, and continues after water boils Decocting 15min, filter, obtain the first filtrate, medicinal residues add water to submerge, and slow fire simmers, and continue to decoct 15min, filter after water boils, Second filtrate, merges twice filtrate, to obtain final product.

The present invention has preferably treatment retention of damp-heat in the interior type ulcerative colitis clinical efficacy, and scientific formula, and compatibility closes Reason, medicine source is abundant, and preparation technology is easy, it is easy to carrying, easy to use, inexpensive effect is good, has no side effect, and safety is high, can be effective Ulcerative colitis clinical symptoms, is the innovation on treatment ulcerative colitis medicine.

Detailed description of the invention

Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.

Embodiment 1

The present invention in the specific implementation, can be made up of the material medicine of following weight meter: Radix Pulsatillae 21g, Pericarpium Granati 16g, Pseudobulbus Bletillae (Rhizoma Bletillae) 21g, Galla Chinensis 16g, Fructus Atriplicis Sibiricae 13g, Rhizoma Coptidis 16g and Radix Sophorae Flavescentis 16g, by above-mentioned raw materials medicament mixed together, add water logging Not yet, soaking 20～30min, slow fire simmers, and continues to decoct 15min, filter, obtain the first filtrate after water boils, and medicinal residues add water to submerge, Slow fire simmers, and continues to decoct 15min, filter, obtain the second filtrate, merge twice filtrate, to obtain final product after water boils.

Embodiment 2

The present invention in the specific implementation, also can be made up of the material medicine of following weight meter: Radix Pulsatillae 25g, Pericarpium Granati 20g, Pseudobulbus Bletillae (Rhizoma Bletillae) 25g, Galla Chinensis 20g, Fructus Atriplicis Sibiricae 16g, Rhizoma Coptidis 17g and Radix Sophorae Flavescentis 18g, add by above-mentioned raw materials medicament mixed together Water logging does not has, and soaks 20～30min, and slow fire simmers, and continues to decoct 15min, filter, obtain the first filtrate after water boils, and medicinal residues add water Submergence, slow fire simmers, and continues to decoct 15min, filter, obtain the second filtrate, merge twice filtrate, to obtain final product after water boils.

Embodiment 3

The present invention in the specific implementation, also can be made up of the material medicine of following weight meter: Radix Pulsatillae 29g, Pericarpium Granati 24g, Pseudobulbus Bletillae (Rhizoma Bletillae) 29g, Galla Chinensis 24g, Fructus Atriplicis Sibiricae 19g, Rhizoma Coptidis 19g and Radix Sophorae Flavescentis 19g, add by above-mentioned raw materials medicament mixed together Water logging does not has, and soaks 20～30min, and slow fire simmers, and continues to decoct 15min, filter, obtain the first filtrate after water boils, and medicinal residues add water Submergence, slow fire simmers, and continues to decoct 15min, filter, obtain the second filtrate, merge twice filtrate, to obtain final product after water boils.

Chinese medicine thinks that ulcerative colitis, initial stage disease position can involve kidney in taste, later stage.Sick just by because of diet the most not Clean or do not save, the thing of the pungent greasy stimulation of addiction, the gas of turbid damp pents up taste, and turbid damp becomes silted up for a long time, burnt in tired resistance, becomes silted up and heat-transformation, under Note large intestine, damp and hot and deposit, block mechanism of qi, stagnation of QI and blood may bring about pain, the stagnation of QI hyperamization stasis of blood, therefore the stagnation of QI, blood stasis, damp and hot three cards mingle and run through disease Overall process, is most difficult to drive away, therefore touching does not heals damp and hot mingling, and recurrent exerbation, the course of disease is longer, and if things go on like this, passages through which vital energy circulates is impaired, heat in blood meat Corruption, therefore clinical ulcerative colitis many companions bloody purulent stool.As the damp and hot elimination later stage the most in time injures taste kidney-Yang, the foundation of acquired constitution cannot Nourishing the congenital foundation, therefore visible kidney yang deficiency.Therapeutic Principle controls based on removing dampness vital energy regualting and blood circulation-promoting, prescription enema coloclysis of the present invention Treating ulcerative colitis, clinical remission symptom effect is notable.Side's medicine has the Radix Pulsatillae, Pericarpium Granati, the Pseudobulbus Bletillae (Rhizoma Bletillae), Galla Chinensis, Fructus Atriplicis Sibiricae, Huang Even, Radix Sophorae Flavescentis composition.

The Radix Pulsatillae in side: nature and flavor hardship temperature, main returns stomach large intestine channel, for clearing away heat-damp and promoting diuresis principal agent, for monarch drug, bitter can heat clearing away, Wen Neng Dampness, damp and hot from basic removal, and can preventing the attack (or recurrence) of malaria dysentery relieving." typhoid fever ": " diarrhea of heat type with tenesmus person, Radix Pulsatillae Decoction master it." modern pharmacology grinds Studying carefully proof, the Radix Pulsatillae can be obviously improved the wound surface ulcer level that ulcerative gastrointestinal is scorching, it is possible to alleviates the disease such as tenesmus, stomachache Shape.

Pericarpium Granati: nature and flavor are sour and astringent, tepor, return Liver Channel, in side, Pericarpium Granati is ministerial drug, puckery intestinal antidiarrheal of holding back, and coordinates the Radix Pulsatillae Play clearing away heat-damp and promoting diuresis, the merit of astringent therapy antidiarrheal altogether." the southern regions of the Yunnan Province book on Chinese herbal medicine ": " control with the passing of time watery diarrhea, simmer clothes with frying Saccharum Sinensis Roxb., control again dysentery pus and blood, large intestine Hematochezia ".Modern pharmacology research proves, Pericarpium Granati includes a large amount of tannic acid, has convergence crevasse, alleviates enteritis, only diarrhoea Effect, can treat the diseases such as exudative ulcer, scald, decubital ulcer, eczema.

Galla Chinensis: sour in the mouth, puckery, cold in nature, return lung, large intestine channel.We are ministerial drug, this medicine acid energy astringent therapy antidiarrheal, cold can heat clearing away, Strengthen the merit of Radix Pulsatillae clearing away heat-damp and promoting diuresis.Modern pharmacological research proves, Galla Chinensis has resistant function to various bacteria, including: gold Staphylococcus aureus, bacillus pyocyaneus, dysentery bacterium, escherichia coli, Bacillus typhi etc..

Rhizoma Coptidis: bitter, cold, GUIXIN, large intestine channel.We are ministerial drug, bitter cold energy clearing away heat-damp and promoting diuresis, eliminating fire and detoxication, coordinate monarch drug altogether Play the merit of clearing away heat-damp and promoting diuresis." typhoid fever ": " TAI YANG syndrome, syndrome of decoction GUI ZHI (Ramulus cinnamomi), cure anti-purgation, and profit is the most more than, and irregularly rapid pulse person, the non-solution of table also, is breathed heavily and antiperspirant The person of going out, Gegen Huangqin Huanglian Tang master it ".Modern pharmacological research, in Rhizoma Coptidis, contained berberine concentration in vitro tests has suppression people many Forming core leukocyte produces the effect of leukotriene B4, can resist Oleum Ricini or diarrhea of mouse that Folium Sennae causes, moreover it is possible to suppression acetic acid The mouse peritoneal caused oozes out increase.It is therefore contemplated that the diarrhea of berberine is relevant with antiinflammatory action.

Radix Sophorae Flavescentis: bitter in the mouth, cold in nature, return liver, kidney channel, we are ministerial drug, only share enhancing clearing away heat-damp and promoting diuresis with the Radix Pulsatillae, Rhizoma Coptidis Dysentery function.Described in " Mingyi Bielu " " nourishing the liver courage, settling five organs, sedate benefit essence, profit nine orifices, except latent heat intestinal, quench the thirst, wake up Wine, yellowish or reddish urine, treat skin ulcer bottom infections, flat gastric qi, make us addiction ".Modern pharmacological research, Radix Sophorae Flavescentis is to rabbit, rat muscle injection oxygen Change matrine 150mg/kg and all have obvious inhibiting effect to being passively or actively skin allergy, and to rabbit anteserum IgE antibody shape Become to have obvious inhibiting effect.

Fructus Atriplicis Sibiricae: bitter, pungent, returns liver, lung meridian.Being adjuvant drug in we, property pungent energy dissipating blood stasis, assistant only helps monarch, ministerial drug promoting blood circulation to remove blood stasis Bitterly." face card guide " mentions that " in order to dissipating depression of QI, on all sides of body, abdominal pain is swollen unbearably, fries perfume (or spice) and enters gas medicine, and the pole of clothes is imitated.Gai Qiqi Perfume (or spice), can lead to strongly fragrant, and can the row of walking crosswise swing, and his medicine non-is through does not stay the person comparable ".Modern study shows that its extracts from crude drugs is to golden yellow Color staphylococcus and colibacillary growth have inhibitory action.

The Pseudobulbus Bletillae (Rhizoma Bletillae): nature and flavor are pungent, bitter, sweet, puckery, it is slightly cold.Return lung, liver, stomach warp.In side, the Pseudobulbus Bletillae (Rhizoma Bletillae) is for making medicine, " Bencao Jingshu ": " white Splendid achnatherum, hardship can expel the heat-evil, pungent can eliminating stagnation, carbuncle all by Ying Qi not from, inverse given birth in meat；Lose the dead flesh of cellulitis impairment of YIN, the hottest heap soil or fertilizer over and around the roots blood stasis institute Cause, thus know main also.Pathogen person in stomach, i.e. pathogenic heat are also；Harmful wind miliaria is slow not to be received, and all blood systems have heat, and the institute of damp-heat and impairment of yin type serious is raw also, enters Blood system is to expel the heat-evil, and eliminating stagnation is by corruption, and the most all cards waste not recover.”

Above-mentioned scientific formula, compatibility is reasonable, can effectively alleviate bloody purulent stool that retention of damp-heat in the interior type ulcerative colitis causes and Rectal distention stimulation is levied, safe and reliable, and curative effect certainly, and has obtained with experiment fully proving by clinical observation, relevant clinical experiment money Expect as follows:

1 clinical data

1.1 diagnostic criteria Western medicine diagnose standards references " diagnosis status of inflammatory bowel disease and the National Consensus for the treatment of " (2012 Guangzhou Chinese Medical Association disease for digest credit meeting inflammatory bowel group)

The diagnosis of ulcerative colitis is comprehensively analyzed mainly in combination with clinical, scope and Histopathologic appearance, row Diagnosis is made on the basis of other noninfectious colitises except infectious.1. clinical manifestation: clinical manifestation for continue or repeatedly The diarrhoea of outbreak, mucopurulent bloody stool companion's stomachache, tenesmus and General Symptoms in various degree, can have the intestinal such as skin, joint, eye Outer performance；2. colonoscopy: ulcerative colitis pathological changes is many from the beginning of rectum, in seriality, diffusivity distribution, show themselves in that Mucosa vascular lake is fuzzy, disorderly or disappearances, hyperemia, edema, matter are crisp, spontaneous or contact bleeding and purulent secretion adhere to, also Common mucosa is coarse, in fine particulate；3. histological examination: active stage: diffusivity acute and chronic cell infiltration in lamina propria, bag Include neutrophilic granulocyte, lymphocyte, plasma cell and eosinophilic granulocyte etc., have around crypt abscess and crypts scorching；Crypts structure Change: crypts is not of uniform size, irregular arrangement, branch-like, goblet cell minimizing etc..Catabasis: mucosal erosion or ulcer are more Close；In lamina propria, neutrophil infiltration reduces or disappears, and lymphocyte, plasmocyte infiltrating, especially at crypt base.Drench Bar folliculus is formed；Crypts structural change: crypts reduces, atrophy, and body of gland shortens, not of uniform size, branch-like, irregular arrangement, cup Shape Leukopenia.The most visible glandular hyperplasia, (left flexure of colon is remote for dysplasia (intraepithelial neoplasia) and paneth's cell metaplasia End).

1.2 TCM syndrome diagnostic criterias

Retention of damp-heat in the interior type ulcerative colitis diagnostic criteria: primary symptom: 1. diarrhoea, mucopurulent bloody stool；2. tenesmus；3. tongue Yellow and greasy fur；4. slippery and rapid pulse or moisten number.Secondary disease: 1. burning sensation of the anus；2. lower abdomen pendant pain or causalgia；3. bitter taste halitosis；4. oliguria with reddish urine； 5. mucosal erosion is hemorrhage；6. mucosa ulcer, periphery is red and swollen, and surface is covered with purulent secretion.Pattern of syndrome determines: possess primary symptom two (Section 1 is indispensable), secondary disease two or primary symptom Section 1, secondary disease 3.

1.3 include in and exclusion standard

Inclusive criteria: diseased region left hemicolon below splenic flexure is dialectical suffers from for retention of damp-heat in the interior type ulcerative colitis Person.

Exclusion standard: 1. merge disease, the psychotics such as severe cardiac, liver, kidney, blood, hormonal system；2. severe, Fulminant UC patient；3. infective enteritis；4. anemia of pregnant woman, women breast-feeding their children, to Salicylic Acid Formulations allergy sufferers；5. there is gastrointestinal surgery History；Do not take medicine by regulation and the judgement person that affects the treatment.

1.4 physical data

Select or be in hospital in December, 2015 from Henan College Of Traditional Chinese Medicine the 3rd Affiliated Hospital's anorectum department outpatient service in January, 2015 Patient 60 example, is grouped the patient meeting inclusive criteria according to medical number random digits table, wherein treatment group 30 example, man 14 Example, female 16 example, age 18～65 years old, average 41.5 years old, the course of disease 0.6～8 years；Matched group 30 example, male 15 examples, female 15 example, the age 18～64 years old, average 42.1 years old, the course of disease 0.8～9 years, the physical data comparing difference such as two groups of Genders, age, courses of disease had no Statistical significance (P ＞ 0.05), has comparability.

1.5 therapeutic scheme

Treatment group gives Chinese medicinal herbs decoction 50ml of the present invention, is heated up to 37～38 DEG C, retention enema after emptying stool before sleeping, Once a day.Matched group gives compound phellodendron bark liquid (Shandong Hanfang Pharmaceutical Co., Ltd., traditional Chinese medicines quasi-word Z10950097) 50ml, adds Temperature, to 37～38 DEG C, empties retention enema after stool, once a day before sleeping.The course for the treatment of is 4 weeks, if patient is administered orally 5-ASA before starting Class medicine then continues to former oral drug therapy.

1.6 observation index and efficacy assessment standard

1.6.1 the transference cure time: observe after treating 4 weeks bloody purulent stool, suffer from diarrhoea, suffer from abdominal pain, the symptom such as tenesmus improves feelings Condition.

1.6.2 under colonoscope, mucosa improves situation: use Rachmilewitz marking system under colonoscope: before and after treatment Colonic pathological change activity scores under all selected patient's scopes is quantified.Specific standards is as follows: 1. mucosa lesions (curing ulcer erosion, Exudate, cellulose, mucus): obvious 4 points, slight 2 points, without 0 point；2. mucosa becomes fragile rapid wear: substantially increase (hematostaxis) 4 points, slight increase (contact bleeding) 2 points, without 0 point；3. granular sensation: have 2 points, without 0 point；4. vascularity: be wholly absent 2 Point, fuzzy disorderly 1 point, normal 0 point.

1.7 untoward reaction evaluations

Record therapeutic process in untoward reaction and evaluate description following characteristics: type, time of origin, frequency, continue time Between, the order of severity.

1.8 statistical procedures

With SPSS 18.0 software, enumeration data is expressed as a percentage, and selects χ²Inspection；Metric results mean ± standard Difference () represent, select t inspection, statistically significant for difference with P ＜ 0.05.

1.9 result

1.9.1 the symptom variation before and after two groups of treatments: after treating 4 weeks, after treatment group stomachache, diarrhoea, bloody purulent stool and pain in the abdomen The symptom such as heavily is improved situation and is all obviously improved (P ＜ 0.05) than matched group, and the transference cure time is substantially better than matched group (P ＜ 0.05) 1, it is shown in Table.

Disease comparitive study (example, sky) before and after 1 liang of table group patient treatment

1.9.2 mark under colonoscope: two groups of patient treatments are marked under postcolon mirror and treat front obvious decline for 4 weeks, Difference statistically significant (P ＜ 0.05), and with the decline degree the most notable (P ＜ 0.05) for the treatment of group, it is shown in Table 2.

Mark under colonoscope before and after 2 liang of table group patient treatment and compare (n,)

1.9.3 in untoward reaction record therapeutic process, treatment group has 1 example patient of short duration slight dizziness, headache occur, stops Take a turn for the better after breath；Matched group has 3 examples burning sensation of the anus sense occur, suffer from diarrhoea and increase the weight of, and takes a turn for the better after drug withdrawal, and two groups all do not occur other serious Untoward reaction.

By clinical observation, enema of the present invention stays enema treatment retention of damp-heat in the interior type ulcerative colitis curative effect reliable, symptom Alleviating substantially, safety is high, without serious adverse reaction.

2 zooperies

2.1 materials and methods

2.1.1 laboratory animal

Selecting healthy cleaning grade wistar male rat 70, between body weight 190～230g, Zhengzhou University's laboratory animal is ground The center that makes internal disorder or usurp provides, and raises in Henan university of TCM animal experimental center, arranges relative humidity 40%～60%, room temperature 20～22 DEG C, illumination every day 8 hours, freely drink water, ingest, conventional raise 7 days to adapt to environment, to avoid affecting animal because of new environment Normal physiological activity.

2.1.2 key instrument and medicine

RM2235 paraffin slicing machine (Germany LEICA company), RM2145 histotome (Leica company of the U.S.), BX4260 Photobiology microscope (Olympus company of Japan)；Ether, dinitrochlorobenzene, acetone (are the raw work biology work in Shanghai Journey Technology Service Co., Ltd produces), mesalazine bolt (Heilongjiang Timehome Pharmaceutical Co., Ltd., the quasi-word of traditional Chinese medicines H20065650,1g/1 piece).

2.1.3 modeling method and animal packet

It is divided into blank group 10 and modeling group 60 according to the method for completely random, poor without significance between each group after packet Different.Blank group rat gives normal dry feed, pure water is fed, and does not give medicine.Modeling group rat peripheral temperature is set as 30 DEG C, humidity is 80%, and gives high fat, high-energy, high lipid food formula: normal feedstuff (20kg), Semen arachidis hypogaeae (5kg), Adeps Sus domestica (5kg), Oleum Sesami (0.15kg), egg (1.2kg), Sal (0.15kg), stir, make strip and block, drink hydromel (200g/L), feeding 2 weeks in such circumstances, losing weight in various degree all occurs in modeling group, matter of defecating is glued, even minority appearance Diarrhoea.Within 15th day, selecting modeling group rat 60, before experiment, fasting 1 day, freely drinks water, and etherization during modeling is big by modeling Mus back wool hair clipper go, and drip with dinitrochlorobenzene (DNCB) (DNCB0.4g: acetone 1mL) and carry on the back, each 5, continuous 14 days, it is seen that Rat abdomen is embrocated position and is occurred red and swollen, pale, hardening, illustrates that rat is to DNCB sensitization.Row dinitrochlorobenzene liquid after sensitization Coloclysis.The flexible pipe of a diameter 2mm selected by coloclysis, selects lumbar anesthesia sleeve pipe, and per anum every day intubates about about 8cm injection thereafter DNCB liquid (DNCB0.1g: acetone 1mL) 0.5mL, continuous coloclysis 4 days, for guaranteeing that the DNCB injected can in the distribution of big enteral all Even and do not spill over, after having filled, rat tails is mentioned, be persistently inverted 30s.Continue to allow rat high caloric diet during coloclysis modeling, Live under hot environment.Observe rat diet situation every day, body weight, and record appearance diarrhoea, symptom of having blood in stool.

10 rat cervical traction dislocation methods blank group and modeling group taken at random are put to death, and light Microscopic observation pathology becomes Change, it was demonstrated that after modeling success, modeling group remains 50 rat random packet: A group: enema normal dose group (10 times of agent of the present invention Amount) 10.B group: enema median dose group (15 multiple dose) of the present invention 10.C group enema of the present invention high dose group (20 times Dosage) 10；D group: mesalazine intestinal thromboembolism treatment group 10；E group: natural recovering group (normal saline) 10.

2.1.4 medication and dosage

Modeling success after, A, B, C group (enema treatment group of the present invention) give respectively normal dose, middle and high dosage this Bright enema coloclysis 2 weeks；D group (western medicine group) uses mesalazine enteric bolt to be dissolved in normal saline enema 2 weeks；E group is (natural Recovery group) use normal saline enema 2 weeks.Proportionately body weight for humans 75kg calculates, and Chinese drug-treated group adult normal's consumption is 400ml every day, According to total dose 112g, can calculate adult and be given daily concentration is 0.28g/ml, show that the every consumption per day of rat is 1.49g.Accordingly Calculate every rat coloclysis requirement, difference coloclysis after concentration.Every pair of medicine is according to 10 times, 15 times, 20 times of difference of dosage Concentrate, concentrate respectively as 40ml (2.8g/ml), 27ml (4.2g/ml) 20ml (5.6g/ml).

2.1.5 observation index

2.1.5.1, during modeling, rat diet situation, body weight change are observed every day, defecate matter, the change of amount, there is no pus Hemafecia and death condition.

2.1.5.2, after modeling success, whole section of colon at rat, will after death be separated, first perusal ulcer area size, Quantity, and record.Pathological changes seriously being located tissue flood take off, tissue slice after embedding, under HE dyes, tissues observed inflammation is invaded Criminal's degree.

2.1.5.3 treatment during, every rat of every day entry enema of the present invention coloclysis consumption, after medication defecate matter, amount, The change of bloody purulent stool, spirit improvement situation, death condition etc..

2.1.5.4 through in, after Western medicine and saline therapy, all rats are put to death, take off whole section of colon, first naked eyes Observe with or without ulcer and ulcer area size, quantity, take off one section just in recovery organization, fixing embedding rear cutout sheet, under HE dyeing Observe pathological changes invasion and attack degree.

2.1.6 standards of grading

2.1.6.1 according to ulcer area size, the degree of tissue injury, with reference to described in Ekstrom GM, Dieleman GM Scale, morphological scoring is shown in Table 3.

Table 3 morphological scoring table

2.1.6.2 damaging infringement degree according to light Microscopic observation colonic ulcer, histological score is shown in Table 4.

Table 4 histological score table

2.1.6.3 according to disease activity index (DAI), stool scoring is shown in Table 4.

Table 4 is defecated character grade form

2.1.6.4 according to disease activity index (DAI), degree of having blood in stool scoring is shown in Table 6.

Table 6 is defecated band blood grade form

2.1.7 statistical procedures

Statistical analysis software: use SPSS18.0 software kit.Data are with mean+SDRepresent, between two groups Relatively use T inspection；Comparing between many groups, the neat person of variance uses one factor analysis of variance, uses LSD inspection between group；Heterogeneity of variance Person uses T ' to check.P ＜ 0.05 represents that difference has statistical significance.

2.2 result

2.2.1 treatment after have blood in stool degree, stool character scoring contrast, be shown in Table 7.

Contrast that table 7 is had blood in stool and character of defecating is marked (n,)

Note: each group compares with normal saline group, * P ＜ 0.05, △ P ＜ 0.05.

2.2.2 morphology after treatment, histological damage score contrast, be shown in Table 8.

Table 8 morphology and histological score contrast (n,)

Note: each group compares with normal saline group, * P ＜ 0.05, △ P ＜ 0.05；Each group compares with Chinese medicine high dose group, P ＜ 0.05.2.3 conclusion

50 rats of this experiment modeling group, take colonic mucosa after modeling success fixing through embedding, and HE dyes, section, and Light Microscopic observation pathological change, discovery blank group Colonic Mucosa is without obvious pathological change, and body of gland is complete, without hemorrhage, edema And inflammatory infiltration, each layer tissue is the most high-visible；A large amount of inflammatory cell infiltrations seen from modeling group rat light Microscopic observation, body of gland is tight Heavily destroy, Submucosa and muscle layer structure disturbance, have the different ulcer surface of obvious degree to be formed, and with serious edema, go out Blood；Treatment by Chinese herbs respectively organize rat according to different concentration coloclysis two weeks after, normal dose group and mesalazine intestinal thrombolytic group light microscopic Seen from lower observation, ulcer level, hemorrhage, edema and body of gland recovery situation relatively modeling group make moderate progress, but still have and go out in various degree Blood, ooze out etc. shows, mucosa and Submucosa, part muscle layer structure disturbance；Median dose group finds the most visible after treatment Hemorrhage, ooze out, ulcer surface degree of injury is also obviously improved, pathomorphism recover the best；High dose group ulcer surface is compared with other Each group is substantially recovered, and edema disappears substantially, and inflammatory cell infiltration is less, and without oozing out, hemorrhage, gland tissue recovers relatively good, respectively Rotating fields can be distinguished, and shows that enema of the present invention substantially alleviates intestinal mucosa inflammation, the diarrhoea that causes ulcerative colitis, just Blood and bloody purulent stool have a better role.

Scientific formula of the present invention, compatibility is reasonable, and medicine source is enriched, and preparation technology is easy, it is easy to carry, easy to use, effect Well, having no side effect, safety is high, can significantly alleviate ulcerative colitis clinical symptoms, has the clinical meaning of reality and good Good application prospect.

Claims (4)

1. the Chinese medicinal herbs decoction treating retention of damp-heat in the interior type ulcerative colitis, it is characterised in that this Chinese medicinal herbs decoction by with The material medicine of lower weight meter is made: Radix Pulsatillae 20-30g, Pericarpium Granati 15-25g, Pseudobulbus Bletillae (Rhizoma Bletillae) 20-30g, Galla Chinensis 15-25g, Fructus Atriplicis Sibiricae Herba chenopodii 12-20g, Rhizoma Coptidis 15-20g and Radix Sophorae Flavescentis 15-20g, add water to submerge by above-mentioned raw materials medicament mixed together, soak 20～ 30min, slow fire simmers, and continues to decoct 15min, filter, obtain the first filtrate after water boils, and medicinal residues add water to submerge, and slow fire simmers, and treat Continue to decoct 15min after water boiling, filter, obtain the second filtrate, merge twice filtrate, to obtain final product.

The Chinese medicinal herbs decoction for the treatment of retention of damp-heat in the interior type ulcerative colitis the most according to claim 1, it is characterised in that by The material medicine of following weight meter is made: Radix Pulsatillae 21g, Pericarpium Granati 16g, Pseudobulbus Bletillae (Rhizoma Bletillae) 21g, Galla Chinensis 16g, Fructus Atriplicis Sibiricae 13g, Rhizoma Coptidis 16g and Radix Sophorae Flavescentis 16g, adds water to submerge by above-mentioned raw materials medicament mixed together, soaks 20～30min, and slow fire simmers, and treats that water boils Rear continuation decocts 15min, filters, obtains the first filtrate, and medicinal residues add water to submerge, and slow fire simmers, and continues to decoct 15min after water boils, Filter, obtain the second filtrate, merge twice filtrate, to obtain final product.

The Chinese medicinal herbs decoction for the treatment of retention of damp-heat in the interior type ulcerative colitis the most according to claim 1, it is characterised in that by The material medicine of following weight meter is made: Radix Pulsatillae 25g, Pericarpium Granati 20g, Pseudobulbus Bletillae (Rhizoma Bletillae) 25g, Galla Chinensis 20g, Fructus Atriplicis Sibiricae 16g, Rhizoma Coptidis 17g and Radix Sophorae Flavescentis 18g, adds water to submerge by above-mentioned raw materials medicament mixed together, soaks 20～30min, and slow fire simmers, and treats that water boils Rear continuation decocts 15min, filters, obtains the first filtrate, and medicinal residues add water to submerge, and slow fire simmers, and continues to decoct 15min after water boils, Filter, obtain the second filtrate, merge twice filtrate, to obtain final product.

The Chinese medicinal herbs decoction for the treatment of retention of damp-heat in the interior type ulcerative colitis the most according to claim 1, it is characterised in that by The material medicine of following weight meter is made: Radix Pulsatillae 29g, Pericarpium Granati 24g, Pseudobulbus Bletillae (Rhizoma Bletillae) 29g, Galla Chinensis 24g, Fructus Atriplicis Sibiricae 19g, Rhizoma Coptidis 19g and Radix Sophorae Flavescentis 19g, adds water to submerge by above-mentioned raw materials medicament mixed together, soaks 20～30min, and slow fire simmers, and treats that water boils Rear continuation decocts 15min, filters, obtains the first filtrate, and medicinal residues add water to submerge, and slow fire simmers, and continues to decoct 15min after water boils, Filter, obtain the second filtrate, merge twice filtrate, to obtain final product.