CN105911686A - Improved micro suction pipe processing system based on optical microscope - Google Patents
Improved micro suction pipe processing system based on optical microscope Download PDFInfo
- Publication number
- CN105911686A CN105911686A CN201610462516.7A CN201610462516A CN105911686A CN 105911686 A CN105911686 A CN 105911686A CN 201610462516 A CN201610462516 A CN 201610462516A CN 105911686 A CN105911686 A CN 105911686A
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- China
- Prior art keywords
- microscope
- micro pipette
- processing system
- dimensional mobile
- optical microscope
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/368—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements details of associated display arrangements, e.g. mounting of LCD monitor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/26—Stages; Adjusting means therefor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention discloses an improved micro suction pipe processing system based on an optical microscope, which mainly comprises the optical microscope, a 30 V adjustable DC voltage-regulated power supply, two three-dimensional mobile stations, a micro suction pipe clamping module, a fused pellet supporting platform module, an electronic eyepiece and a liquid crystal display. The fused pellet technology is adopted to process the end part of the micro suction pipe, the view field of the microscope is transferred to the liquid crystal display screen, and the system of the invention has the following main advantages that the micro suction pipe can be conveniently and simply processed, and the precision is high; the screen is used for displaying the image, eyes of the operator can be protected conveniently and quickly, and the labor intensity is reduced; the magnitude of current outputted by the current source can be adjusted, the micro suction pipe processing speed can be controlled conveniently, and the processing effects are significantly improved; the system can be widely applied to various micro suction pipe processing experiments based on the microscope, the original microscope operation mode is changed, the eyes of the operator are well protected, the operation labor intensity is reduced, and the ergonomics principle can be met.
Description
Technical field
The present invention relates to processing system based on microscope transformation, a kind of optically-based microscope is transformed
Micro pipette processing system, there is convenient and swift, to alleviate operator's labor intensity feature.
Technical background
So far, most experimentation is required under high power microscope see modern biotechnology scientific technological advance
Examine the form of cell, activity, vital movement etc., sometimes also need to use the mode of fixing cell to carry out next
The observation of step and operation, utilize the former of hydraulic pressure control in testing such as BFP (Biomembrane Force Probe)
Reason, uses capillary micro pipette to fix individual cells and operates.In this type of is tested, the head of capillary micro pipette
Portion should carry out smooth mellow and full process, it is to avoid destroys cellularity fixing cell when.The most on the market
Existing micro pipette datatron is expensive, inefficiency, and the eyes of operator are easily because dig-inning for a long time
Field of microscope and produce fatigue, it is therefore necessary to develop a set of conveniently micro pipette processing system.
Summary of the invention
It is an object of the invention to provide the micro pipette processing system of a kind of optically-based microscope transformation.This is
System high-amplification-factor, the Stereo microscope of big opereating specification are amplified, and use high accuracy three-dimensional mobile station
Operation micro pipette and melted bead process.
For achieving the above object, the technical solution used in the present invention is:
The micro pipette processing system of the present invention optically-based microscope transformation, including optical microscope, 30V can
Tuning DC voltage-stabilized source, two pieces of three-dimensional mobile stations, fixed platform, micro pipette, micro pipette grip block, melted
Bead module, melted bead module grip block, electronic eyepiece, liquid crystal display, wire, trip bolt；
Optical microscope, 30V adjustable D. C regulated and liquid crystal display are both secured on work platforms,
Fixed platform is fixed on the base of optical microscope by trip bolt, and two three-dimensional mobile stations are by fastening
Screw is respectively symmetrically the two ends being fixed on fixed platform；Melted bead module grip block is fixed by trip bolt
In left three-dimensional mobile station, melted bead module is fixed on melted bead module grip block；Micro pipette clamps
Block is fixed in right three-dimensional mobile station by trip bolt, and micro pipette adhesive tape is fixed on micro pipette grip block；
The end of described micro pipette is relative with melted bead module, and the both positive and negative polarity of 30V adjustable D. C regulated leads to
Cross wire and connect the two poles of the earth of melted bead module respectively；Electronic eyepiece inserts an eyepiece mirror of optical microscope
In Tong, the other end is connected on liquid crystal display by USB connector.
Described optical microscope is Motic company SMZ-168 model optics Stereo microscope, and zoom scope is
0.75 ×～5 ×.
Described 30V adjustable D. C regulated is that 305DM tetra-shows D.C. regulated power supply, and current precision can
Reach 0.1mA.
Described three-dimensional mobile platform is Physik Instrument company M105 three-dimensional mobile station.
Described electronic eyepiece is Anyty DKMC01 electronic eyepiece, and sensitive chip is 5,000,000 true color CMOS,
0.5 times of type minifier.
The micro pipette processing system of optically-based microscope transformation is to use melted bead technology by micro pipette end
Process, and field of microscope transferred on LCD Panel, this must possess following some require:
1, the total magnification of this system is the picture that Motic microscopical eyepiece amplification is multiplied by LCD Panel
Element amplification, this general times must be more than 250 times；2, optical microscope and the Z-direction of three-dimensional mobile station
Displacement enough, must must ensure that the head of micro pipette and melted bead can be gathered at microscope by mobile
Focal plane on；3, the effect in order to make melted bead process micro pipette reaches optimal, the minimum of three-dimensional mobile station
Displacement must be sufficiently small, and preferably its precision is 0.1 micron, adds thermocurrent and also allows for being adjusted to properly
Sizes values；When 4, carrying out micro pipette process operation, it is necessary to electric current to be heated finally stable, the form of bead
Carrying out after no longer there is significant change, this process takes around the time of 1～2 second.
The invention have the advantages that:
Present invention is mainly used for capillary micro pipette head in life science and process problem, utilize degree of precision
Three-dimensional mobile platform control micro pipette and the relative position of melted bead, field of microscope is transferred to liquid crystal
On display screen, have the advantage that
1) easy to operate simply, field-of-view image is directly perceived；
2) three-dimensional mobile station Minimum sliding distance is little, and precision is high, it is easy to control the treatment effect of micro pipette；Separately
The speed that can be processed by the size control of regulation heating power supply outward and quality, have high controllability；
3) field of microscope is transferred in LCDs, ensureing that amplification and image are the most basic
On, reduce the labor intensity of operator to the full extent, protect the eyes of operator well.
Accompanying drawing explanation
Fig. 1 is the system structure schematic diagram of the present invention, and (a) (b) (c) (d) is respectively different visual angles knot
Composition.
Fig. 2 is front elevational schematic of the present invention.
Fig. 3 is micro pipette self-clamping module structural representation of the present invention.
Fig. 4 is that the present invention melts bead module grip block structural representation.
Fig. 5 is three-dimensional mobile station structural representation of the present invention.
In figure: 1, Motic optical microscope, 2,30V adjustable D. C regulated, 3, Physic Instrument
Company M105 three-dimensional mobile station, 4, M105 fixed platform, 5, micro pipette, 6, micro pipette grip block, 7,
Melted bead module, 8, melted bead module grip block, 9, Anyty electronic eyepiece, 10, liquid crystal display,
11, wire, 12, trip bolt.
Detailed description of the invention
The invention will be further described with example below in conjunction with the accompanying drawings.
As it is shown in figure 1, the present invention includes Motic optical microscope 1,30V adjustable D. C regulated 2,2
Individual Physic Instrument company M105 three-dimensional mobile station 3, M105 fixed platform 4, micro pipette 5, micro-
Suction pipe grip block 6, melted bead module 7, melted bead module grip block 8, Anyty electronic eyepiece 9, liquid
Crystal display 10,11,12 pieces of trip bolts 12 of some wires.
As shown in Figure 1 and Figure 2, Motic optical microscope 1,30V adjustable D. C regulated 2 and liquid crystal
Display 10 is fixed on the correct position of work platforms.M105 fixed platform 4 is solid by trip bolt 12
It is scheduled on the base of Motic optical microscope 1.Two Physik Instrument company M105 three-dimensionals move
Platform 3 is respectively symmetrically the two ends being fixed on M105 fixed platform 4 by trip bolt.Melted bead module clamping
Block 8 is fixed in left M105 three-dimensional mobile station 3 by trip bolt 12, and melted bead module 7 is fixed on
On melted bead module grip block 8.Micro pipette grip block 6 is fixed on right M105 tri-by trip bolt 12
In dimension mobile station 3, micro pipette 5 is fixed on micro pipette grip block 6 by adhesive tape.Two wires 11 are respectively
Connect both positive and negative polarity and the two poles of the earth of melted bead module 7 of 30V adjustable D. C regulated 2.Anyty electronics
Eyepiece 9 inserts in Motic optical microscope 1 in one of them eyepiece lens barrel, and the other end is connected by USB
Mouth is connected on liquid crystal display 10.This system total magnification is more than 250 times.
Described optical microscope 1 is Motic company SMZ-168 model optics Stereo microscope, zoom scope
Be 0.75 ×～5 ×.
Described 30V adjustable D. C regulated (2) is that 305DM tetra-shows D.C. regulated power supply, electric current essence
Degree is up to 0.1mA.
Described three-dimensional mobile platform is Physik Instrument company M105 three-dimensional mobile station, single shaft stroke
18mm, precision is up to 0.1 μm.
Described electronic eyepiece 9 is Anyty DKMC01 electronic eyepiece, and sensitive chip is 5,000,000 true color
CMOS, 0.5 times of type minifier.
The operation principle of the present invention is as follows:
Motic optical microscope 1 is connected power supply, the power of light and angle adjustment to optimal.By 30V
The plug of adjustable D. C regulated 2 inserts in 220V power supply, adjusts and outputs current to desired value.Take off through
Micro pipette blank after drawing pin instrument to break process is put in micro pipette grip block 6 and is fixed with adhesive tape, and adjustment is stretched out
It is fixed after length.Two M105 three-dimensional mobile platform 3 about simultaneously adjusting, make melted bead and micro-suction
Tube head is slowly close and does not contacts, and opens liquid crystal display 10 to naked eyes when of cannot differentiating again, according to
Enlarged drawing picture on display continue to adjust M105 three-dimensional mobile platform 3 by melted bead and micro pipette head it
Between distance adjust within 100 microns.Step on foot switch connection power supply bead is heated, liquid crystal
Display 10 shows the alteration of form of bead, treats that slowly rotating right M105 three-dimensional after this change is stably moves
Moving platform Y-axis feeding, uniformly inserts the head of micro pipette among melted bead, sees liquid crystal display 10
In be not inserted into the raw capillarity of distribution in the middle part of the micro pipette in melted bead after unclamp foot switch at once, disconnect
Electric current, rotates right M105 three-dimensional mobile platform 3Y axle and outwards extracts micro pipette, and micro pipette can be in capillarity
Fluid column fracture topmost, fracture port is smooth mellow and full.
The micro pipette that the number of times of the degree of depth and insertion that micro pipette inserts melted bead depends on finally testing straight
Footpath size, if micro pipette is used for capturing the adhesion bead of diameter 2 μm, then the degree of depth and number of times are all adjusted to less
Being worth proper, if capturing the erythrocyte of a diameter of 6 μm, then the degree of depth and number of times can be adjusted to higher value.
Add the principle that heat fusing bead utilizes current flowing resistance to generate heat, firing rate and effect and resistance wire is big
Little relevant with size of current, a suitable size of current can be found interval, further through repeating experiment
Increase the convenience of experiment.
The when that the image of liquid crystal display 10 display not clearly cannot be carried out experiment, adjust Motic light
Learn the distance between microscope 1 object lens and melted bead, target is placed on focal plane.It addition, adjust
Picture exposure and white balance, also can play assosting effect.
This experiment should be noted during perusal micro pipette and melted bead are close, it is necessary to assure both
Slowly near and do not contact, it is to avoid destroy micro pipette.During experiment, size of current is unsuitable too high, it is to avoid cause electricity
The situation that resistance silk is aglow.Once melt the position of bead fixing after, follow-up repetition has only in experiment every time
Adjust micro pipette near melted bead.
After whole experiment completes, cutting off all power supplys, microscope puts protective cover and preserves.
Described above is micro pipette processing system detailed of optically-based microscope a kind of to present invention transformation
Thin introduction, is adapted to assist in method and the thought understanding the present invention.Guarantor in spirit and claims of the present invention
In the range of protecting, any modifications and changes making the present invention, within both falling within protection scope of the present invention.
Claims (8)
1. the micro pipette processing system of an optically-based microscope transformation, it is characterised in that: include optical microscope
(1), 30V adjustable D. C regulated (2), two three-dimensional mobile stations (3), fixed platform (4), micro-
Suction pipe (5), micro pipette grip block (6), melted bead module (7), melted bead module grip block (8),
Electronic eyepiece (9), liquid crystal display (10), wire (11), trip bolt (12)；
Optical microscope (1), 30V adjustable D. C regulated (2) and liquid crystal display (10) are the most fixing
On work platforms, fixed platform (4) is fixed on optical microscope (1) by trip bolt (12)
On base, two three-dimensional mobile stations (3) are respectively symmetrically by trip bolt and are fixed on fixed platform (4)
Two ends；Melted bead module grip block (8) is fixed in left three-dimensional mobile station by trip bolt, melted little
Ball module (7) is fixed on melted bead module grip block (8)；Micro pipette grip block (6) is by fastening
Screw is fixed in right three-dimensional mobile station, and micro pipette (5) adhesive tape is fixed on micro pipette grip block (6)；
The end of described micro pipette is relative with melted bead module, 30V adjustable D. C regulated (2) positive and negative
Pole connects the two poles of the earth of melted bead module (7) respectively by wire (12)；Electronic eyepiece (9) inserts light
Learning in an eyepiece lens barrel of microscope (1), the other end is connected to liquid crystal display by USB connector
(10) on.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: the total magnification of this system is that the eyepiece amplification of optical microscope is multiplied by LCD Panel
Pixel amplification, described total magnification is more than 250 times.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: described optical microscope and the Z-direction displacement sufficiently large (being 200mm to the maximum) of three-dimensional mobile station
Make micro pipette end and melted bead all can be in Jiao of optical microscope by the movement of three-dimensional mobile station
On face.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: the mobile accuracy of described three-dimensional mobile station is 0.1 micron.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: described optical microscope (1) is Motic company SMZ-168 model optics Stereo microscope, becomes
Times scope is 0.75 ×～5 ×.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: described 30V adjustable D. C regulated (2) is that 305DM tetra-shows D.C. regulated power supply, electricity
Stream precision is up to 0.1mA.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
It is: described three-dimensional mobile station is Physik Instrument company M105 three-dimensional mobile station.
The micro pipette processing system of optically-based microscope the most according to claim 1 transformation, its feature
Being: described electronic eyepiece (9) is AnytyDKMC01 electronic eyepiece, sensitive chip is 5,000,000 true
Color cmos, 0.5 times of type minifier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610462516.7A CN105911686A (en) | 2016-06-21 | 2016-06-21 | Improved micro suction pipe processing system based on optical microscope |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610462516.7A CN105911686A (en) | 2016-06-21 | 2016-06-21 | Improved micro suction pipe processing system based on optical microscope |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105911686A true CN105911686A (en) | 2016-08-31 |
Family
ID=56759423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610462516.7A Pending CN105911686A (en) | 2016-06-21 | 2016-06-21 | Improved micro suction pipe processing system based on optical microscope |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105911686A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110987767A (en) * | 2019-11-11 | 2020-04-10 | 中国科学院地质与地球物理研究所 | Air pressure micromanipulation system and method for micron-sized particle sample |
| CN111089971A (en) * | 2019-12-11 | 2020-05-01 | 浙江大学 | Protein interaction quantitative detection device under membrane potential regulation and control |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6363750B1 (en) * | 2000-02-25 | 2002-04-02 | Chris D. Chiodo | Automatic pipette puller and forge |
| CN102453667A (en) * | 2010-10-26 | 2012-05-16 | 陈浩杰 | Microscope fixing pin manufacturing method and microscope fixing pin manufactured by same |
| CN102453668A (en) * | 2010-10-26 | 2012-05-16 | 陈浩杰 | Microscope injection pin manufacturing method and microscope injection pin manufactured by same |
-
2016
- 2016-06-21 CN CN201610462516.7A patent/CN105911686A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6363750B1 (en) * | 2000-02-25 | 2002-04-02 | Chris D. Chiodo | Automatic pipette puller and forge |
| CN102453667A (en) * | 2010-10-26 | 2012-05-16 | 陈浩杰 | Microscope fixing pin manufacturing method and microscope fixing pin manufactured by same |
| CN102453668A (en) * | 2010-10-26 | 2012-05-16 | 陈浩杰 | Microscope injection pin manufacturing method and microscope injection pin manufactured by same |
Non-Patent Citations (3)
| Title |
|---|
| 倪萌萌: "小鼠胚胎显微操作针的制作方法", 《江苏农业科学》 * |
| 冯蕾: "显微操作针制作方法的探讨", 《细胞与分子免疫学杂志》 * |
| 刘天军: "细胞操作用锻针器的设计", 《机械设计》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110987767A (en) * | 2019-11-11 | 2020-04-10 | 中国科学院地质与地球物理研究所 | Air pressure micromanipulation system and method for micron-sized particle sample |
| CN111089971A (en) * | 2019-12-11 | 2020-05-01 | 浙江大学 | Protein interaction quantitative detection device under membrane potential regulation and control |
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Application publication date: 20160831 |