CN105911233A - Determination method for zinc content of Guiling jelly - Google Patents

Determination method for zinc content of Guiling jelly Download PDF

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Publication number
CN105911233A
CN105911233A CN201610229809.0A CN201610229809A CN105911233A CN 105911233 A CN105911233 A CN 105911233A CN 201610229809 A CN201610229809 A CN 201610229809A CN 105911233 A CN105911233 A CN 105911233A
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CN
China
Prior art keywords
sample
solution
standard
zinc
guiling gao
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610229809.0A
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Chinese (zh)
Inventor
杨柳
吴婕
李镇坤
李倩雯
岑昕霖
陈东红
黄敏
谭略
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Wuzhou product quality inspection institute
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Wuzhou product quality inspection institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wuzhou product quality inspection institute filed Critical Wuzhou product quality inspection institute
Priority to CN201610229809.0A priority Critical patent/CN105911233A/en
Publication of CN105911233A publication Critical patent/CN105911233A/en
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/02—Food
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44—Sample treatment involving radiation, e.g. heat

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention provides a determination method for the zinc content of Guiling jelly, and aims to provide a method characterized by simplicity, accurate detection result and no need of large instrument for determination of the zinc content of Guiling jelly. The technical scheme consists of: 1) preparation of a standard solution; 2) pretreatment of a sample; 3) sample digestion; and 4) determination. The method belongs to the technical field of chemical detection.

Description

A kind of measure the method for Zn content in GUILING GAO
Technical field
The invention discloses the assay method of a kind of Zn content, specifically, be a kind of method measuring zinc element content； Belong to technical field of chemical detection.
Background technology
GUILING GAO powder is the time-honored Traditional health care in Wuzhou, Guangxi food, its warm in nature and, the coolest the driest, there is heat clearing away and go Wet, prosperous blood granulation promoting, loosening bowel to relieve constipation, skin care such as is refreshed oneself at the effect, all-ages, thus extremely people like, and World-wide Sale.Testudinis Siberian cocklebur Cream powder with Carapax et Plastrum Testudinis, bean jelly (starch, Herba mesonae chinensis extracting solution), Radix Angelicae Sinensis, Rhizoma Smilacis Glabrae, Radix Glycyrrhizae, Flos Lonicerae and Herba Taraxaci as primary raw material It is made, also can be equipped with the Chinese crude drugs such as Flos Chrysanthemi, Folium Eriobotryae, Fructus Momordicae and make the product that a series of taste is different with function, cater to The taste of consumer groups.
Summary of the invention
It is an object of the invention to provide a kind of detection method simple, testing result is accurate, without using the survey of large-scale instrument Determine the method for Zn content in GUILING GAO.
For solving above-mentioned technical problem, the technical scheme that the present invention provides is such that
A kind of measuring the method for Zn content in GUILING GAO, its assay method comprises the steps: successively
1) preparation of standard solution:
Draw zinc standard solution, concentration is 0.0,100.0,200.0,300.0,400.0, the standard of 500.0ng/ml molten Liquid；
2) pretreatment of sample
GUILING GAO sample blender blends, standby；
3) the clearing up of sample:
Weigh GUILING GAO sample 5～10g in porcelain crucible, crucible is placed on electric furnace, is evaporated, carbonization, it is transferred to high temperature In stove, 550 DEG C of more than constant temperature 2h；After taking-up, cooling, add in 5ml concentrated nitric acid sample ash in crucible, crucible is placed in electric furnace On, it is evaporated, then proceeds in 550 DEG C of high temperature furnaces, continue ashing 1～2h, be white lime shape to sample；Cooling is taken out from high temperature furnace After, with nitric acid dissolved samples ash, solution is moved in 50ml volumetric flask, fully mix after constant volume, be test liquid；Meanwhile, press Said method does blank；
4) measure
Start, adjusts instrument plasma flow 15.0L/min, auxiliary flow 0.2L/min, nebulizer flow 0.80L/ Min, radio-frequency power 1300W, sample flow 1.50L/min, observed range 15.0 axial observation plasma reads parameter temporal 5 ～20S, reagent blank, standard series, sample solution are introduced instrument respectively, by computer drawing standard curve, calculates and return Equation, draws the concentration of sample solution.
Further, above-mentioned a kind of measuring the method for Zn content in GUILING GAO, zinc concentration is 1000 μ g/ml.
Further, above-mentioned a kind of the method for Zn content in GUILING GAO, the concentration of described zinc standard solution are measured It is 10 μ g/ml.
Further, above-mentioned a kind of measure the method for Zn content, step 3 in GUILING GAO) described in nitric acid volume fraction It is 1%.
Compared with prior art, element zinc in GUILING GAO is measured by the technical scheme that the present invention provides, detection method letter Single, testing result is reliable, without using the method for Zn content in the mensurations GUILING GAO of large-scale instrument, be conducive to raising product matter Amount.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the claim of the present invention is done the most in detail
Illustrate, but do not constitute any limitation of the invention, any in the claims in the present invention protection
The amendment of the limited number of time that scope is made, still within the claims of the present invention.
Embodiment 1
1, reagent
Concentrated nitric acid
The nitric acid (volume fraction) of 1%
Standard reserving solution: zinc (C=1000 μ g/ml)
Standard solution: zinc (C=10 μ g/ml)
2, instrument condition
3, experimental technique
(1), the preparation of standard solution: draw each standard solution, be configured to the nitric acid of 1%, zinc concentration is 0.0, 100.0,200.0,300.0,400.0, the standard solution of 500.0ng/ml；
(2), the pretreatment of sample: GUILING GAO sample blender blends, standby.
(3), the clearing up of sample: weigh GUILING GAO sample 5～10g in porcelain crucible, crucible is placed on electric furnace, carefully steams Dry, carbonization, to not smoldering, is transferred in high temperature furnace, 550 DEG C of more than constant temperature 2h.Take out, cooling after, add 5ml concentrated nitric acid in In sample ash in crucible, crucible is placed on electric furnace, is carefully evaporated, then proceed in 550 DEG C of high temperature furnaces, continue ashing 1～2h, It is white lime shape to sample.After taking out cooling from high temperature furnace, with nitric acid (volume fraction is 1%) dissolved samples ash, solution is moved Enter in 50ml volumetric flask, fully mix after constant volume, be test liquid.Meanwhile, blank is done as stated above.
4, measure
Start, when instrument vacuum reaches requirement, adjusts instrument indices, makes instrumental sensitivity, resolution etc. each After item index reaches requirement, edit assay method.Reagent blank, standard series, sample solution are introduced instrument respectively, by calculating Standard curve drawn by machine, calculates regression equation, draws the concentration of sample solution.
In order to better illustrate advantages of the present invention, be given below the present invention provide several groups of method detection data:
Table one: the comparison of each sampling amount
Sampling amount (g) 1.0 5.0 10.0 15.0 20.0
Zn(mg/kg) 1.377 1.142 1.287 1.126 0.829
Note: each sampling amount is all settled to 50ml.
Table two: each element range of linearity
Element μg/L
Zn 0～500
Table three: each detection limits
Element μg/L
Zn 1.5
Table four: the recovery of standard addition of each sample

Claims (4)

1. one kind measures the method for Zn content in GUILING GAO, it is characterised in that its assay method comprises the steps: successively
1) preparing standard solution:
Draw zinc standard solution, with the nitric acid of 1% be configured to zinc concentration be 0.0,100.0,200.0,300.0,400.0, The standard solution of 500.0ng/ml；
2) pretreatment of sample
GUILING GAO sample blender blends, standby；
3) the clearing up of sample:
Weigh GUILING GAO sample 5～10g in porcelain crucible, crucible is placed on electric furnace, is evaporated, carbonization, it is transferred in high temperature furnace, 550 DEG C of more than constant temperature 2h；After taking-up, cooling, add in 5ml concentrated nitric acid sample ash in crucible, crucible is placed on electric furnace, steam Dry, then proceed in 550 DEG C of high temperature furnaces, continue ashing 1～2h, be white lime shape to sample；After taking out cooling from high temperature furnace, use nitre Acid dissolved samples ash, moves into solution in 50ml volumetric flask, fully mixes after constant volume, be test liquid；Meanwhile, as stated above Do blank；
4) measure
Start, adjustment instrument plasma flow 15.0L/min, auxiliary flow 0.2L/min, nebulizer flow 0.80L/min, Radio-frequency power 1300W, sample flow 1.50L/min, observed range 15.0 axial observation plasma read parameter temporal 5～ 20S, introduces instrument respectively by reagent blank, standard series, sample solution, by computer drawing standard curve, calculates recurrence side Journey, draws the concentration of sample solution.
The method of a kind of zinc element content simultaneously measured in GUILING GAO the most according to claim 1, it is characterised in that institute The zinc standard reserving solution concentration stated is 1000 μ g/ml.
The most according to claim 1 a kind of measure the method for Zn content in GUILING GAO, it is characterised in that described zinc standard The concentration using liquid is 10 μ g/ml.
The most according to claim 1 a kind of measure the method for Zn content in GUILING GAO, it is characterised in that step 3) described in Nitric acid volume fraction is 1%.
CN201610229809.0A 2016-04-14 2016-04-14 Determination method for zinc content of Guiling jelly Pending CN105911233A (en)

Priority Applications (1)

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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
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Publication Number Publication Date
CN105911233A true CN105911233A (en) 2016-08-31

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202649120U (en) * 2012-06-26 2013-01-02 广东省潮州市质量计量监督检测所 Food detecting system
CN103940897A (en) * 2014-05-13 2014-07-23 广州金域医学检验中心有限公司 Method for determining traces of mercury, cadmium, lead and arsenic in food by inductively coupled plasma mass spectrometry (ICP-MS)

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202649120U (en) * 2012-06-26 2013-01-02 广东省潮州市质量计量监督检测所 Food detecting system
CN103940897A (en) * 2014-05-13 2014-07-23 广州金域医学检验中心有限公司 Method for determining traces of mercury, cadmium, lead and arsenic in food by inductively coupled plasma mass spectrometry (ICP-MS)

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
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冯翠萍等: "《食品卫生学实验指导》", 31 July 2014, 中国轻工业出版社 *
刘磊等: "电感耦合等离子体-原子发射光谱法测定中药中微量元素", 《光谱实验室》 *
刘素华等: "电感耦合等离子体原子发射光谱法测定食品中铅、镉、铬、镍的方法研究", 《预防医学论坛》 *
姜杰等: "电感耦合等离子体发射光谱法同时测定食品中8种元素", 《卫生研究》 *
孙玲玲等: "海带中12中元素ICP-OES检测的三种前处理方法对比研究", 《海洋科学》 *
张胜帮等: "电感藕合等离子体原子发射光谱法测定酸枣仁汤中多种微量元素", 《温州大学学报》 *
李明等: "《化学设计创新实验》", 28 February 2014, 云南大学出版社 *
沈小玲等: "干法灰化－ICP－OES法测定奶粉中的微量元素", 《中国化工贸易》 *
王守箐: "电感耦合等离子发射光谱法测定金银花中的九种微量元素", 《化学工程师》 *
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Application publication date: 20160831