CN105907532A - Manufacturing method of distillery yeast and distillery yeast - Google Patents
Manufacturing method of distillery yeast and distillery yeast Download PDFInfo
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- CN105907532A CN105907532A CN201610487044.0A CN201610487044A CN105907532A CN 105907532 A CN105907532 A CN 105907532A CN 201610487044 A CN201610487044 A CN 201610487044A CN 105907532 A CN105907532 A CN 105907532A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
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- C12G3/02—Preparation of other alcoholic beverages by fermentation
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Abstract
The invention provides a manufacturing method of a distillery yeast and a distillery yeast. The distillery yeast is prepared from the following raw materials in parts by weight: 3 parts of barley, 4 parts of wheat, 1.5 parts of soybean and 1.5 parts of pea. The following yeast seeds are adopted: an Aspergillus oryzae box yeast seed accounts for 0.1% of the raw materials, an Aspergillus flavus box yeast seed accounts for 0.1% of the raw materials, and an Aspergillus niger box yeast seed accounts for 0.1% of the raw materials. The manufacturing method of the distillery yeast comprises the following steps: inoculating the raw materials composed of the barley, wheat, soybean and pea into the Aspergillus oryzae box yeast seed, Aspergillus flavus box yeast seed and Aspergillus niger box yeast seed, uniformly mixing, sending into a ventilating yeast tank, regulating the temperature to 30-35 DEG C, and carrying out stationary culture for 6-8 hours; after the yeast materials enter the tank, culturing for 12 hours, carrying out primary yeast turning, and keeping at the normal temperature 30-35 DEG C; after continuing culturing for 4-6 hours, carrying out secondary yeast turning in time; culturing for 24-28; and drying. The distillery yeast has stable quality, avoids the defects in the prior art for preparing the yeast from crude materials, and shortens the manufacturing period.
Description
Technical field
The present invention relates to manufacture method and the distillers yeast of a kind of distillers yeast.
Background technology
Daqu (massive raw stater for alcholic liquor) is the main saccharifying ferment of traditional liquor, yellow wine, vinegar brewing technique, and it is with pure Semen Tritici aestivi or by one
The Fructus Hordei Vulgaris of certainty ratio cooperation, Semen Pisi sativi etc. are raw material, and size-reduced adding water is pressed into brick-shaped curved billet, is positioned in deep closet
The various wild mushrooms relying on nature to bring into are cultivated on raw material naturally, it is thus achieved that the various microorganisms being of value to fermentation,
Cultivation cycle 21 days, then through air-dry Daqu (massive raw stater for alcholic liquor);The Daqu (massive raw stater for alcholic liquor) made is through the 3-6 storage of individual month, referred to as Massa Medicata Fermentata.
Daqu (massive raw stater for alcholic liquor) is that the multiple-microorganism by nature participates in fermentation, defines the multiple enzyme that saccharifying amylase is main body
System.Owing to when Daqu (massive raw stater for alcholic liquor) makes, raw material is raw material yeast production, ripe song various enzyme system is impure and enzyme activity is the highest.Yeast production institute
Semen Tritici aestivi (content of starch 66-72%, protein content 10-13%), Fructus Hordei Vulgaris (content of starch 63-70%, protein
Content 10%), starch mass-energy in the raw material such as Semen Pisi sativi (content of starch 57%, protein content 24.6%) is by microorganism
Utilize, and protein is not over high-temperature denatured, cannot utilize and residue in product during the fermentation in yeast making process
Product affect the sense organ of its product.
Daqu (massive raw stater for alcholic liquor) is that the wild microorganism by nature participates in fermentation, thus also form and be much unfavorable for the micro-of fermentation
Biology, the most often there is a bent bad phenomenon in Qu Haoyi room, room in this, and the yeast production time is long, defines difficult quality control
The phenomenons such as system.
Summary of the invention
In order to make distillers yeast have stable quality, it is to avoid the defect that prior art raw material yeast production exists, shorten and make week
Phase, the present invention proposes manufacture method and the distillers yeast of a kind of distillers yeast.
To this end, the present invention is by the following technical solutions.
A kind of manufacture method of distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 3 parts, Semen Tritici aestivi 4 parts, Semen sojae atricolor 1.5
Part, Semen Pisi sativi 1.5 parts;The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰,
Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰;Specifically comprise the following steps that
(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible
Reducing powder (because Semen Tritici aestivi viscosity is big), it is 40-60 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, then, Semen sojae atricolor, Semen Pisi sativi respectively
Putting in 75-90 DEG C of hot water and soak 1-2 hour, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added into the tap water of mass percent 40%
Middle material moistening 1-2 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, in the steaming of 0.2-0.3MPa pressure
Steaming 10-15 minute in batch can, discharging is cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters in the ventilation koji bed of sterilizing room to train
Support as becoming bent;
(2) cultivation of bent box yeast inoculation:
(2.1) cultivation of aspergillus oryzae (As3.800):
(2.1.1) slant tube spawn culture (one-level kind)
Culture medium can add 2.5 grams of agar, by well-established law system with 89 ° of Be ' rice-koji juice or beerwort 100 milliliters
Become solid test tube slant culture medium;After aseptically accessing original seed, cultivate in 28-30 DEG C of constant incubator
72-80 hour;It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4-6 DEG C;
(2.1.2) triangular flask amplification culture (two grades of kinds)
Take fresh wheat bran and add water 85% by quality, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks,
Every bottle of bent material thickness about 1 centimetre, is stoppered tampon, and bottleneck kraft paper is wrapped up, in high-pressure sterilizing pot with
0.15-0.2MPa pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain,
One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, and knot
Detain bottle after cake, continue to cultivate 72-80 hour;4 DEG C need not be put in the cultured Triangle-botde koji short time
Depositing standby in refrigerator, the time is less than one week;
(2.1.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
Become bent material;I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method be first by the alcoholic fermented product starter with
After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists
30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature
Rising to 35-37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, then
Load song to close, bent material thickness 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth,
To ensure the humidity of song material;Indoor temperature should control at 28-30 DEG C, and after 10 hours, mycelia starts the most numerous
Growing, temperature rises to 34-35 DEG C, bent material molding, can turn over song;Method is carefully by the song of molding with bamboo chip
Carrying out turn-over, turn over song and to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature exists
30-36 DEG C, promote mycelial growth spore, altogether through 72-96 hour, the yellow spore of bent material is the most aging, i.e.
Song can be dried or shine song;Drying deep closet temperature and bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag,
Put dry indoor to save backup;
(2.2) cultivation of Aspergillus flavus (AS3.951)
(2.2.1) slant tube spawn culture (one-level kind)
Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, and solubility is formed sediment
20 grams of powder, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, beyond the Great Wall
Tampon, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30-40 minute under 0.15-0.2MPa steam pressure,
Take out and be put into slant tube while hot, move on in constant incubator after waiting test tube slant cooling, it is ensured that temperature 36-37 DEG C,
Constant temperature culture 72-80 hour, to measure slant tube culture medium whether aseptic inoculation, the slant tube good by sterilizing
Aseptically access Aspergillus flavus test tube original seed, an original seed switching slant tube 20;Connecting original seed
Test tube be positioned in the calorstat of 28-30 DEG C cultivation 72-80 hour, the inclined-plane that now should grow tries
It is standby that pipe strain is put in preservation in 4 DEG C of refrigerators;
(2.2.2) the triangular flask alcoholic fermented product starter cultivates (two grades of kinds)
Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle
Thickness is 1-1.5 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15-0.2MPa in high-pressure sterilizing pot
Pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain, a slant tube
Access in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, through 34 hours, detain bottle after molding, continue
Continuous cultivate 72-80 hour;4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time deposit
Standby, deposit and not can exceed that one week;
(2.2.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter
After closing uniformly, then be spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature at 30-32 DEG C, then
Covering with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature has been raised to 35-37 DEG C,
Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness
Spend 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material;
Temperature temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 34-35 DEG C, bent
Expect molding, song can be turned over;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry
Cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C, promote mycelial growth spore,
Altogether through 72-96 hour, the yellow green spore of bent material is the most aging, can dry song or shine song;Dry deep closet temperature to bring up to
40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life
More than 3 months;
(2.3) cultivation of Aspergillus niger (AS3.324)
(2.3.1) slant tube spawn culture (one-level kind)
Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate
1 gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving
Tampon beyond the Great Wall, outsourcing kraft paper, put in pressure cooker sterilizing 30-40 under vapour pressure is with 0.15-0.2MPa pressure
Minute, to take out and be put into inclined-plane while hot, cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure
Inclined-plane examination medium sterilization is the most thorough;Inoculation, aseptically accesses test tube slant tube good for sterilizing former
Kind, a test tube original seed is transferred 20, and the slant tube inoculated is put in 28-30 DEG C of constant incubator and cultivated
72-80 hour, it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube;(2.3.2) Triangle-botde koji is cultivated
(two grades of kinds)
Take fresh wheat bran (thickness is moderate) and add water 80%, make water suction uniform after turning, be then charged into 500 milliliter three
In the bottle of angle, every bottle of bent material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, in high-pressure sterilizing pot
With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain,
One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, treats
Detain bottle after molding, continue to cultivate 72-80 hour;In the cultured Triangle-botde koji short time need not, can put
Deposit in entering 4 DEG C of refrigerators, the resting period not can exceed that one week standby;
(2.3.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material
After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with
The gunnysack of sterilizing covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, rice-koji
Mould spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness
1.5 2 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material;
Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, bent material
Molding, can turn over song;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry
Cotton cloth, covers wet cotton cloth, and to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether
Through 72-96 hour, the black spore of bent material was the most aging, can dry song or shine song;Dry deep closet temperature to bring up to
40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life
More than 3 months;
(3) aerated koji making cultivates (production yeast inoculation)
The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation,
Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore
Germinate, static gas wave refrigerator 6-8 hour;Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling,
Control temperature between 30 35 DEG C;
Enter pond after 12 hours cultivate, carry out for the first time turning over song, make bent material loose, reduce flowing resistance, protect
Hold normal temperature 30-35 DEG C;After continuing to cultivate 4-6 hour, carry out second time in time and turn over song;Incubation time is to 24-28
Hour;
(4) song is become to dry
Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.
A kind of distillers yeast, uses above-mentioned manufacture method to prepare.
Beneficial effect: the source distillers yeast of song is with Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor, Semen Pisi sativi as raw material, adds certainly after crushed
Water after high temperature steaming, drop to room temperature add in aseptic deep closet the aspergillus oryzae with pure culture, Aspergillus flavus,
The microorganism fungus kinds such as Aspergillus niger are enriched with, and amplification culture forms, incubation time after 28-32 hour again through wind
The dry Cheng Qu being maturation, its contained fermentation composition is fully achieved or exceedes composition and effect of tradition Daqu (massive raw stater for alcholic liquor),;
This song is to add the multiple major microorganisms strain of zythepsary, except rich in saccharifying amylase, also creating greatly
The neutrality of amount, acid protease, become amino acids the breaks down proteins in raw material, for yeast in brewing process
The growth of bacterium provides the nutrition of nitrogen source, also enriches the mouthfeel of product, shorten yeast production simultaneously while improving liquor output rate
Incubation time, moreover it is possible to well control into song quality;The present invention has the advantage that and can be summarized as:
(1) the raw material yeast-making technology of distillers yeast (Daqu (massive raw stater for alcholic liquor)) changes grog yeast-making technology into, by traditional production cycle 23
It shortens to 28-36 hour;
(2) multi-cultur es utilizing pure culture works in coordination with yeast production, produces significantly carrying of multiple enzyme system particularly prolease activity
High;
(3) saccharifying enzymic activity u/g >=500;Neutral, acid protease activity u/g >=200.
Detailed description of the invention
A kind of manufacture method of distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 300kg, Semen Tritici aestivi 400kg, Semen sojae atricolor
150kg, Semen Pisi sativi 150kg;The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰,
Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰.Specifically comprise the following steps that
(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible
Reducing powder (because Semen Tritici aestivi viscosity is big), it is 40 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, and then, Semen sojae atricolor, Semen Pisi sativi is put respectively
Entering in 75-90 DEG C of hot water and soak 1 hour, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added in the tap water of mass percent 40% profit
Expect 1 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, steaming in the material steaming tank of 0.2MPa pressure
10 minutes, discharging was cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters to cultivate in the ventilation koji bed of sterilizing room as becoming bent;
(2) cultivation of bent box yeast inoculation:
(2.1) cultivation of aspergillus oryzae (As3.800):
(2.1.1) slant tube spawn culture (one-level kind)
Culture medium can add 2.5 grams of agar with 100 milliliters of 89 ° of B ' e rice-koji juice, makes solid examination by well-established law
Pipe slant medium;After aseptically accessing original seed, cultivate 72 hours in 28 DEG C of constant incubators;
It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4 DEG C;
(2.1.2) triangular flask amplification culture (two grades of kinds)
Take fresh wheat bran and add water 85% by quality, make water suction uniform after turning, be then charged into 500 milliliters of triangular flasks
In, every bottle of bent material thickness 1 centimetre, it is stoppered tampon, bottleneck kraft paper is wrapped up, with 0.15MPa in high-pressure sterilizing pot
Pressure sterilization 30 minutes, takes out cooling, aseptically accesses slant tube strain, and a slant tube connects
Enter in 20 bottles of triangular flasks, then put in 28 DEG C of constant incubators, through 34 hours, after molding, detain bottle, continue to cultivate
72 hours;4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby, the time
Less than one week;
(2.1.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room,
Become bent material;I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method be first by the alcoholic fermented product starter with
After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists
30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature
Rising to 37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into
Qu He, bent material thickness 1.5 centimetres, then cover sterilized wet cotton cloth on the shelf on song material, to ensure
The humidity of bent material;Indoor temperature should control at 28 DEG C, and after 10 hours, mycelia starts amount reproduction, temperature
Rise to 35 DEG C, bent material molding, song can be turned over;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over
Bent to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 32 DEG C, promotion mycelia
Growth spore, altogether through 72 hours, the yellow spore of bent material is the most aging, can dry song or shine song;Baking deep closet temperature
Can bring up to 40 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup;
(2.2) cultivation of Aspergillus flavus (AS3.951)
(2.2.1) slant tube spawn culture (one-level kind)
Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, and solubility is formed sediment
20 grams of powder, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, beyond the Great Wall
Tampon, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30 minutes under 0.15MPa steam pressure, take out while hot
Being put into slant tube, move on in constant incubator, it is ensured that temperature 36-37 DEG C after waiting test tube slant cooling, constant temperature is trained
Support 72 hours, to measure slant tube culture medium whether aseptic inoculation, by slant tube good for sterilizing at aseptic bar
Aspergillus flavus test tube original seed, an original seed switching slant tube 20 is accessed under part;The test tube connecting original seed is put
It is placed in the calorstat of 28-30 DEG C cultivation 72 hours, now the slant tube strain grown should be put in
In 4 DEG C of refrigerators, preservation is standby;
(2.2.2) the triangular flask alcoholic fermented product starter cultivates (two grades of kinds)
Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle
Thickness is 1 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15MPa pressure in high-pressure sterilizing pot
Sterilizing 30 minutes, takes out cooling, aseptically accesses slant tube strain, and a slant tube accesses 20
In bottle triangular flask, then put in 28 DEG C of constant incubators, through 34 hours, after molding, detain bottle, continue to cultivate 72-80
Hour;4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby, deposit not
Can exceed that one week;
(2.2.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter
After closing uniformly, then be spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature at 30-32 DEG C, then
Covering with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature has been raised to 37 DEG C,
Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness
Spend 1.5 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material;
Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 35 DEG C, and bent material is
Molding, can turn over song;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry cotton
Gauze, covers wet cotton cloth, and to ensure humidity, temperature, at 32 DEG C, promotes mycelial growth spore, altogether through 72
Hour, the yellow green spore of bent material is the most aging, can dry song or shine song;Dry deep closet temperature and bring up to 40 DEG C, the alcoholic fermented product starter
Moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, more than 3 months shelf-life;
(2.3), the cultivation of Aspergillus niger (AS3.324)
(2.3.1) slant tube spawn culture (one-level kind)
Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate
1 gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving
Tampon beyond the Great Wall, outsourcing kraft paper, put in pressure cooker sterilizing 30 minutes under vapour pressure is with 0.15MPa pressure,
Taking out and be put into inclined-plane while hot, cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure inclined-plane examination
Medium sterilization is the most thorough;Inoculation, aseptically accesses test tube original seed slant tube good for sterilizing, and one
Propping up test tube original seed to transfer 20, the slant tube inoculated is put in 28-30 DEG C of constant incubator and is cultivated 72 hours
, it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube;
(2.3.2) Triangle-botde koji cultivates (two grades of kinds)
Take fresh wheat bran (thickness is moderate) and add water 80%, make water suction uniform after turning, be then charged into 500 milliliter three
In the bottle of angle, every bottle of bent material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, in high-pressure sterilizing pot
With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain,
One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, treats
Detain bottle after molding, continue to cultivate 72-80 hour;In the cultured Triangle-botde koji short time need not, can put
Deposit in entering 4 DEG C of refrigerators, the resting period not can exceed that one week standby;
(2.3.3) bent box yeast inoculation cultivates (three grades of kinds)
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 60 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material
After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with
The gunnysack of sterilizing covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, rice-koji
Mould spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness
1.5 2 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material;
Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, bent material
Molding, can turn over song;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry
Cotton cloth, covers wet cotton cloth, and to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether
Through 72-96 hour, the black spore of bent material was the most aging, can dry song or shine song;Dry deep closet temperature to bring up to
40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life
More than 3 months;
(3) aerated koji making cultivates (production yeast inoculation)
The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation,
Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore
Germinate, static gas wave refrigerator 6-8 hour;Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling,
Control temperature between 30 35 DEG C;
Song expects that pond, after 12 hours cultivate, carries out turning over song for the first time, makes bent material loose, reduces flowing resistance,
Keep normal temperature 30-35 DEG C;After continuing to cultivate 4-6 hour, carry out second time in time and turn over song;Incubation time arrives
24-28 hour;
(4) song is become to dry
Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.
The explanation of former strain and source:
Aspergillus oryzae (As3.800): main product saccharifying type enzyme activity, is brewing yellow rice wine saccharifying agent aspergillosis;
Yellow aspergillosis (As3.951): mainly produce neutral protease vigor, saccharifying enzymic activity;
Aspergillus niger (As3.324): main product saccharifying type enzyme activity, acid protease activity;
Strain derives from Beijing City Food Brewing Research Institute.
Selecting the Fructus Hordei Vulgaris of raw material, Semen Tritici aestivi, Semen sojae atricolor, Semen Pisi sativi is starter-making materials, be for growth provide good nitrogen source,
The nutrition parts such as carbon source, promote that microorganism well grows and the formation of enzyme vigor.
Unit symbol ° B ' e shown in above-mentioned refers to: Baume concentration.
Claims (2)
1. a manufacture method for distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 3 parts, Semen Tritici aestivi 4 parts, Semen sojae atricolor
1.5 parts, Semen Pisi sativi 1.5 parts;The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰,
Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰;Specifically comprise the following steps that
(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible subtract
Few powder (because Semen Tritici aestivi viscosity is big), it is 40-60 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, and then, Semen sojae atricolor, Semen Pisi sativi is respectively put into
Soaking 1-2 hour in 75-90 DEG C of hot water, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added into material moistening in the tap water of mass percent 40%
1-2 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, in the material steaming tank of 0.2-0.3MPa pressure steam
Expecting 10-15 minute, discharging is cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters to cultivate in the ventilation koji bed of sterilizing room as becoming bent;
(2) cultivation of bent box yeast inoculation:
(2.1) cultivation of aspergillus oryzae:
(2.1.1) slant tube spawn culture
Culture medium 8-9 ° of B ' e rice-koji juice or beerwort 100 milliliters, add 2.5 grams of agar, makes solid examination by well-established law
Pipe slant medium;After aseptically accessing original seed, cultivate 72-80 hour in 28-30 DEG C of constant incubator
?;It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4-6 DEG C;
(2.1.2) triangular flask amplification culture
Take fresh wheat bran and add water about 85% by quality, make water suction uniform after turning, be then charged into 500 milliliters of triangles
In bottle, every bottle of bent material thickness 1-1.5 centimetre, it is stoppered tampon, bottleneck kraft paper is wrapped up, in high-pressure sterilizing pot
With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain,
One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, and knot
Detain bottle after cake, continue to cultivate 72-80 hour;4 DEG C need not be put in the cultured Triangle-botde koji short time
Depositing standby in refrigerator, the time is less than one week;
(2.1.3) bent box yeast inoculation is cultivated
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
Become bent material;I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method be first by the alcoholic fermented product starter with
After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists
30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature
Rising to 35-37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, then
Load song to close, bent material thickness 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth,
To ensure the humidity of song material;Indoor temperature should control at 28-30 DEG C, and after 10 hours, mycelia starts the most numerous
Growing, temperature rises to 34-35 DEG C, bent material molding, can turn over song;Method is carefully by the song of molding with bamboo chip
Carry out turn-over, turn over song and to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C,
Promoting mycelial growth spore, altogether through 72-96 hour, the yellow spore of bent material is the most aging, can dry song or shine song;
Drying deep closet temperature and can bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry room
Inside save backup;
(2.2) cultivation of Aspergillus flavus
(2.2.1) slant tube spawn culture
Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, soluble starch
20 grams, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, cotton beyond the Great Wall
Plug, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30-40 minute under 0.15-0.2MPa steam pressure, take out
It is put into slant tube while hot, moves on in constant incubator after waiting test tube slant cooling, it is ensured that temperature 35-37 DEG C, permanent
Temperature is cultivated 72-80 hour, to measure slant tube culture medium whether aseptic inoculation, is existed by the slant tube that sterilizing is good
Aspergillus flavus test tube original seed, an original seed switching slant tube 20 is accessed under aseptic condition;Connecting original seed
Test tube is positioned in the calorstat of 28-30 DEG C cultivation 72-80 hour, the slant tube bacterium that now should will grow
It is standby that kind is put in preservation in 4 DEG C of refrigerators;
(2.2.2) the triangular flask alcoholic fermented product starter is cultivated
Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle
Thickness is 1-1.5 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15-0.2MPa in high-pressure sterilizing pot
Pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain, a slant tube
Access in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, after 34h, molding, detain bottle, continue training
Support 72-80 hour;4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby
With, deposit and not can exceed that one week;
(2.2.3) bent box yeast inoculation is cultivated
Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality;Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter
After closing uniformly, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then uses
Sterilized gunnysack covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35-37 DEG C,
Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness
Spend 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material;
Temperature temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 34-35 DEG C, bent
Expect molding, song can be turned over;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry
Cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C, promote mycelial growth spore,
Altogether through 72-96 hour, the yellow green spore of bent material is the most aging, can dry song or shine song;Dry deep closet temperature to bring up to
40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life
More than 3 months;
(2.3), the cultivation of Aspergillus niger
(2.3.1) slant tube spawn culture
Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate 1
Gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving and fill in
Upper tampon, outsourcing kraft paper, put in pressure cooker sterilizing 30-40 under vapour pressure is with 0.15-0.2MPa pressure and divide
Clock, takes out and is put into inclined-plane while hot, and cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure inclined-plane
Examination medium sterilization is the most thorough;Inoculation, aseptically accesses test tube original seed slant tube good for sterilizing,
One test tube original seed is transferred 20, and the slant tube inoculated puts into cultivation 72-80 in 28-30 DEG C of constant incubator
Hour, it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube;(2.3.2) Triangle-botde koji is cultivated
Take fresh wheat bran and add water 80%, make water suction uniform after turning, be then charged in 500ml triangular flask, every bottle of song
Material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, presses with 0.15-0.2MPa in high-pressure sterilizing pot
Power sterilizing 30-40 minute, takes out cooling, aseptically accesses slant tube strain, and a slant tube connects
Enter in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, through 34 hours, after molding, detain bottle, continue
Continuous cultivate 72-80 hour;In the cultured Triangle-botde koji short time need not, 4 DEG C of refrigerator internal memories can be put into
Put, the resting period not can exceed that one week standby;
(2.3.3) bent box yeast inoculation is cultivated
Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate,
Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room,
I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material
After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with going out
The gunnysack of bacterium covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, aspergillus oryzae
Spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness 1.5-2
Centimetre, then cover sterilized wet cotton cloth on the shelf on song material, to ensure the humidity of song material;Temperature exists
28-30 DEG C, after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, and song expects molding,
Song can be turned over;Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry cotton cloth,
Covering wet cotton cloth, to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether through 72-96
Hour, the black spore of bent material is the most aging, can dry song or shine song;Dry deep closet temperature and bring up to 40-45 DEG C, bent
Kind moisture, below 12%, bandages with plastic bag, puts dry indoor and save backup, more than 3 months shelf-life;
(3) aerated koji making is cultivated
The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation,
Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore
Germinate, static gas wave refrigerator 6-8 hour;Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling,
Control temperature between 30-35 DEG C;
Song expects that pond, after 12 hours cultivate, carries out turning over song for the first time, makes bent material loose, reduces flowing resistance,
Keep normal temperature 30-35 DEG C;After continuing to cultivate 4-6 hour, carry out second time in time and turn over song;Incubation time arrives
24-28 hour;
(4) song is become to dry
Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.
2. a distillers yeast, it is characterised in that: it is to use the manufacture method described in claim 1 to prepare.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106119011A (en) * | 2016-09-08 | 2016-11-16 | 黑龙江北大仓集团有限公司 | The preparation method of Beijing University's storehouse wine high-temperature daqu |
CN107151610A (en) * | 2017-07-24 | 2017-09-12 | 宁夏彭阳县添龄黄酒酿造有限责任公司 | A kind of yellow wine brewing method |
CN109423386A (en) * | 2017-08-24 | 2019-03-05 | 百世威酒业(漳州)有限公司 | A kind of yellow rice wine and natural yeast of delicate fragrance type is mixed steams technique |
-
2016
- 2016-06-28 CN CN201610487044.0A patent/CN105907532A/en active Pending
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106119011A (en) * | 2016-09-08 | 2016-11-16 | 黑龙江北大仓集团有限公司 | The preparation method of Beijing University's storehouse wine high-temperature daqu |
CN107151610A (en) * | 2017-07-24 | 2017-09-12 | 宁夏彭阳县添龄黄酒酿造有限责任公司 | A kind of yellow wine brewing method |
CN109423386A (en) * | 2017-08-24 | 2019-03-05 | 百世威酒业(漳州)有限公司 | A kind of yellow rice wine and natural yeast of delicate fragrance type is mixed steams technique |
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