CN105907532A - Manufacturing method of distillery yeast and distillery yeast - Google Patents

Abstract

The invention provides a manufacturing method of a distillery yeast and a distillery yeast. The distillery yeast is prepared from the following raw materials in parts by weight: 3 parts of barley, 4 parts of wheat, 1.5 parts of soybean and 1.5 parts of pea. The following yeast seeds are adopted: an Aspergillus oryzae box yeast seed accounts for 0.1% of the raw materials, an Aspergillus flavus box yeast seed accounts for 0.1% of the raw materials, and an Aspergillus niger box yeast seed accounts for 0.1% of the raw materials. The manufacturing method of the distillery yeast comprises the following steps: inoculating the raw materials composed of the barley, wheat, soybean and pea into the Aspergillus oryzae box yeast seed, Aspergillus flavus box yeast seed and Aspergillus niger box yeast seed, uniformly mixing, sending into a ventilating yeast tank, regulating the temperature to 30-35 DEG C, and carrying out stationary culture for 6-8 hours; after the yeast materials enter the tank, culturing for 12 hours, carrying out primary yeast turning, and keeping at the normal temperature 30-35 DEG C; after continuing culturing for 4-6 hours, carrying out secondary yeast turning in time; culturing for 24-28; and drying. The distillery yeast has stable quality, avoids the defects in the prior art for preparing the yeast from crude materials, and shortens the manufacturing period.

Description

The manufacture method of a kind of distillers yeast and distillers yeast

Technical field

The present invention relates to manufacture method and the distillers yeast of a kind of distillers yeast.

Background technology

Daqu (massive raw stater for alcholic liquor) is the main saccharifying ferment of traditional liquor, yellow wine, vinegar brewing technique, and it is with pure Semen Tritici aestivi or by one The Fructus Hordei Vulgaris of certainty ratio cooperation, Semen Pisi sativi etc. are raw material, and size-reduced adding water is pressed into brick-shaped curved billet, is positioned in deep closet The various wild mushrooms relying on nature to bring into are cultivated on raw material naturally, it is thus achieved that the various microorganisms being of value to fermentation, Cultivation cycle 21 days, then through air-dry Daqu (massive raw stater for alcholic liquor)；The Daqu (massive raw stater for alcholic liquor) made is through the 3-6 storage of individual month, referred to as Massa Medicata Fermentata.

Daqu (massive raw stater for alcholic liquor) is that the multiple-microorganism by nature participates in fermentation, defines the multiple enzyme that saccharifying amylase is main body System.Owing to when Daqu (massive raw stater for alcholic liquor) makes, raw material is raw material yeast production, ripe song various enzyme system is impure and enzyme activity is the highest.Yeast production institute Semen Tritici aestivi (content of starch 66-72%, protein content 10-13%), Fructus Hordei Vulgaris (content of starch 63-70%, protein Content 10%), starch mass-energy in the raw material such as Semen Pisi sativi (content of starch 57%, protein content 24.6%) is by microorganism Utilize, and protein is not over high-temperature denatured, cannot utilize and residue in product during the fermentation in yeast making process Product affect the sense organ of its product.

Daqu (massive raw stater for alcholic liquor) is that the wild microorganism by nature participates in fermentation, thus also form and be much unfavorable for the micro-of fermentation Biology, the most often there is a bent bad phenomenon in Qu Haoyi room, room in this, and the yeast production time is long, defines difficult quality control The phenomenons such as system.

Summary of the invention

In order to make distillers yeast have stable quality, it is to avoid the defect that prior art raw material yeast production exists, shorten and make week Phase, the present invention proposes manufacture method and the distillers yeast of a kind of distillers yeast.

To this end, the present invention is by the following technical solutions.

A kind of manufacture method of distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 3 parts, Semen Tritici aestivi 4 parts, Semen sojae atricolor 1.5 Part, Semen Pisi sativi 1.5 parts；The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰, Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰；Specifically comprise the following steps that

(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible Reducing powder (because Semen Tritici aestivi viscosity is big), it is 40-60 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, then, Semen sojae atricolor, Semen Pisi sativi respectively Putting in 75-90 DEG C of hot water and soak 1-2 hour, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added into the tap water of mass percent 40% Middle material moistening 1-2 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, in the steaming of 0.2-0.3MPa pressure Steaming 10-15 minute in batch can, discharging is cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters in the ventilation koji bed of sterilizing room to train Support as becoming bent；

(2) cultivation of bent box yeast inoculation:

(2.1) cultivation of aspergillus oryzae (As3.800):

(2.1.1) slant tube spawn culture (one-level kind)

Culture medium can add 2.5 grams of agar, by well-established law system with 89 ° of Be ' rice-koji juice or beerwort 100 milliliters Become solid test tube slant culture medium；After aseptically accessing original seed, cultivate in 28-30 DEG C of constant incubator 72-80 hour；It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4-6 DEG C；

(2.1.2) triangular flask amplification culture (two grades of kinds)

Take fresh wheat bran and add water 85% by quality, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, Every bottle of bent material thickness about 1 centimetre, is stoppered tampon, and bottleneck kraft paper is wrapped up, in high-pressure sterilizing pot with 0.15-0.2MPa pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain, One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, and knot Detain bottle after cake, continue to cultivate 72-80 hour；4 DEG C need not be put in the cultured Triangle-botde koji short time Depositing standby in refrigerator, the time is less than one week；

(2.1.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, Become bent material；I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method be first by the alcoholic fermented product starter with After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists 30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature Rising to 35-37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, then Load song to close, bent material thickness 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, To ensure the humidity of song material；Indoor temperature should control at 28-30 DEG C, and after 10 hours, mycelia starts the most numerous Growing, temperature rises to 34-35 DEG C, bent material molding, can turn over song；Method is carefully by the song of molding with bamboo chip Carrying out turn-over, turn over song and to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature exists 30-36 DEG C, promote mycelial growth spore, altogether through 72-96 hour, the yellow spore of bent material is the most aging, i.e. Song can be dried or shine song；Drying deep closet temperature and bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, Put dry indoor to save backup；

(2.2) cultivation of Aspergillus flavus (AS3.951)

(2.2.1) slant tube spawn culture (one-level kind)

Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, and solubility is formed sediment 20 grams of powder, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, beyond the Great Wall Tampon, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30-40 minute under 0.15-0.2MPa steam pressure, Take out and be put into slant tube while hot, move on in constant incubator after waiting test tube slant cooling, it is ensured that temperature 36-37 DEG C, Constant temperature culture 72-80 hour, to measure slant tube culture medium whether aseptic inoculation, the slant tube good by sterilizing Aseptically access Aspergillus flavus test tube original seed, an original seed switching slant tube 20；Connecting original seed Test tube be positioned in the calorstat of 28-30 DEG C cultivation 72-80 hour, the inclined-plane that now should grow tries It is standby that pipe strain is put in preservation in 4 DEG C of refrigerators；

(2.2.2) the triangular flask alcoholic fermented product starter cultivates (two grades of kinds)

Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle Thickness is 1-1.5 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15-0.2MPa in high-pressure sterilizing pot Pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain, a slant tube Access in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, through 34 hours, detain bottle after molding, continue Continuous cultivate 72-80 hour；4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time deposit Standby, deposit and not can exceed that one week；

(2.2.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter After closing uniformly, then be spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature at 30-32 DEG C, then Covering with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature has been raised to 35-37 DEG C, Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness Spend 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material； Temperature temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 34-35 DEG C, bent Expect molding, song can be turned over；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry Cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C, promote mycelial growth spore, Altogether through 72-96 hour, the yellow green spore of bent material is the most aging, can dry song or shine song；Dry deep closet temperature to bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life More than 3 months；

(2.3) cultivation of Aspergillus niger (AS3.324)

(2.3.1) slant tube spawn culture (one-level kind)

Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate 1 gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving Tampon beyond the Great Wall, outsourcing kraft paper, put in pressure cooker sterilizing 30-40 under vapour pressure is with 0.15-0.2MPa pressure Minute, to take out and be put into inclined-plane while hot, cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure Inclined-plane examination medium sterilization is the most thorough；Inoculation, aseptically accesses test tube slant tube good for sterilizing former Kind, a test tube original seed is transferred 20, and the slant tube inoculated is put in 28-30 DEG C of constant incubator and cultivated 72-80 hour, it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube；(2.3.2) Triangle-botde koji is cultivated (two grades of kinds)

Take fresh wheat bran (thickness is moderate) and add water 80%, make water suction uniform after turning, be then charged into 500 milliliter three In the bottle of angle, every bottle of bent material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, in high-pressure sterilizing pot With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain, One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, treats Detain bottle after molding, continue to cultivate 72-80 hour；In the cultured Triangle-botde koji short time need not, can put Deposit in entering 4 DEG C of refrigerators, the resting period not can exceed that one week standby；

(2.3.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with The gunnysack of sterilizing covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, rice-koji Mould spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness 1.5 2 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material； Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, bent material Molding, can turn over song；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry Cotton cloth, covers wet cotton cloth, and to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether Through 72-96 hour, the black spore of bent material was the most aging, can dry song or shine song；Dry deep closet temperature to bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life More than 3 months；

(3) aerated koji making cultivates (production yeast inoculation)

The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation, Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore Germinate, static gas wave refrigerator 6-8 hour；Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling, Control temperature between 30 35 DEG C；

Enter pond after 12 hours cultivate, carry out for the first time turning over song, make bent material loose, reduce flowing resistance, protect Hold normal temperature 30-35 DEG C；After continuing to cultivate 4-6 hour, carry out second time in time and turn over song；Incubation time is to 24-28 Hour；

(4) song is become to dry

Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.

A kind of distillers yeast, uses above-mentioned manufacture method to prepare.

Beneficial effect: the source distillers yeast of song is with Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor, Semen Pisi sativi as raw material, adds certainly after crushed Water after high temperature steaming, drop to room temperature add in aseptic deep closet the aspergillus oryzae with pure culture, Aspergillus flavus, The microorganism fungus kinds such as Aspergillus niger are enriched with, and amplification culture forms, incubation time after 28-32 hour again through wind The dry Cheng Qu being maturation, its contained fermentation composition is fully achieved or exceedes composition and effect of tradition Daqu (massive raw stater for alcholic liquor),； This song is to add the multiple major microorganisms strain of zythepsary, except rich in saccharifying amylase, also creating greatly The neutrality of amount, acid protease, become amino acids the breaks down proteins in raw material, for yeast in brewing process The growth of bacterium provides the nutrition of nitrogen source, also enriches the mouthfeel of product, shorten yeast production simultaneously while improving liquor output rate Incubation time, moreover it is possible to well control into song quality；The present invention has the advantage that and can be summarized as:

(1) the raw material yeast-making technology of distillers yeast (Daqu (massive raw stater for alcholic liquor)) changes grog yeast-making technology into, by traditional production cycle 23 It shortens to 28-36 hour；

(2) multi-cultur es utilizing pure culture works in coordination with yeast production, produces significantly carrying of multiple enzyme system particularly prolease activity High；

(3) saccharifying enzymic activity u/g >=500；Neutral, acid protease activity u/g >=200.

Detailed description of the invention

A kind of manufacture method of distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 300kg, Semen Tritici aestivi 400kg, Semen sojae atricolor 150kg, Semen Pisi sativi 150kg；The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰, Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰.Specifically comprise the following steps that

(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible Reducing powder (because Semen Tritici aestivi viscosity is big), it is 40 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, and then, Semen sojae atricolor, Semen Pisi sativi is put respectively Entering in 75-90 DEG C of hot water and soak 1 hour, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added in the tap water of mass percent 40% profit Expect 1 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, steaming in the material steaming tank of 0.2MPa pressure 10 minutes, discharging was cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters to cultivate in the ventilation koji bed of sterilizing room as becoming bent；

(2) cultivation of bent box yeast inoculation:

(2.1) cultivation of aspergillus oryzae (As3.800):

(2.1.1) slant tube spawn culture (one-level kind)

Culture medium can add 2.5 grams of agar with 100 milliliters of 89 ° of B ' e rice-koji juice, makes solid examination by well-established law Pipe slant medium；After aseptically accessing original seed, cultivate 72 hours in 28 DEG C of constant incubators； It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4 DEG C；

(2.1.2) triangular flask amplification culture (two grades of kinds)

Take fresh wheat bran and add water 85% by quality, make water suction uniform after turning, be then charged into 500 milliliters of triangular flasks In, every bottle of bent material thickness 1 centimetre, it is stoppered tampon, bottleneck kraft paper is wrapped up, with 0.15MPa in high-pressure sterilizing pot Pressure sterilization 30 minutes, takes out cooling, aseptically accesses slant tube strain, and a slant tube connects Enter in 20 bottles of triangular flasks, then put in 28 DEG C of constant incubators, through 34 hours, after molding, detain bottle, continue to cultivate 72 hours；4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby, the time Less than one week；

(2.1.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room, Become bent material；I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method be first by the alcoholic fermented product starter with After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists 30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature Rising to 37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into Qu He, bent material thickness 1.5 centimetres, then cover sterilized wet cotton cloth on the shelf on song material, to ensure The humidity of bent material；Indoor temperature should control at 28 DEG C, and after 10 hours, mycelia starts amount reproduction, temperature Rise to 35 DEG C, bent material molding, song can be turned over；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over Bent to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 32 DEG C, promotion mycelia Growth spore, altogether through 72 hours, the yellow spore of bent material is the most aging, can dry song or shine song；Baking deep closet temperature Can bring up to 40 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup；

(2.2) cultivation of Aspergillus flavus (AS3.951)

(2.2.1) slant tube spawn culture (one-level kind)

Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, and solubility is formed sediment 20 grams of powder, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, beyond the Great Wall Tampon, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30 minutes under 0.15MPa steam pressure, take out while hot Being put into slant tube, move on in constant incubator, it is ensured that temperature 36-37 DEG C after waiting test tube slant cooling, constant temperature is trained Support 72 hours, to measure slant tube culture medium whether aseptic inoculation, by slant tube good for sterilizing at aseptic bar Aspergillus flavus test tube original seed, an original seed switching slant tube 20 is accessed under part；The test tube connecting original seed is put It is placed in the calorstat of 28-30 DEG C cultivation 72 hours, now the slant tube strain grown should be put in In 4 DEG C of refrigerators, preservation is standby；

(2.2.2) the triangular flask alcoholic fermented product starter cultivates (two grades of kinds)

Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle Thickness is 1 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15MPa pressure in high-pressure sterilizing pot Sterilizing 30 minutes, takes out cooling, aseptically accesses slant tube strain, and a slant tube accesses 20 In bottle triangular flask, then put in 28 DEG C of constant incubators, through 34 hours, after molding, detain bottle, continue to cultivate 72-80 Hour；4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby, deposit not Can exceed that one week；

(2.2.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter After closing uniformly, then be spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature at 30-32 DEG C, then Covering with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature has been raised to 37 DEG C, Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness Spend 1.5 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material； Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 35 DEG C, and bent material is Molding, can turn over song；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry cotton Gauze, covers wet cotton cloth, and to ensure humidity, temperature, at 32 DEG C, promotes mycelial growth spore, altogether through 72 Hour, the yellow green spore of bent material is the most aging, can dry song or shine song；Dry deep closet temperature and bring up to 40 DEG C, the alcoholic fermented product starter Moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, more than 3 months shelf-life；

(2.3), the cultivation of Aspergillus niger (AS3.324)

(2.3.1) slant tube spawn culture (one-level kind)

Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate 1 gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving Tampon beyond the Great Wall, outsourcing kraft paper, put in pressure cooker sterilizing 30 minutes under vapour pressure is with 0.15MPa pressure, Taking out and be put into inclined-plane while hot, cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure inclined-plane examination Medium sterilization is the most thorough；Inoculation, aseptically accesses test tube original seed slant tube good for sterilizing, and one Propping up test tube original seed to transfer 20, the slant tube inoculated is put in 28-30 DEG C of constant incubator and is cultivated 72 hours , it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube；

(2.3.2) Triangle-botde koji cultivates (two grades of kinds)

Take fresh wheat bran (thickness is moderate) and add water 80%, make water suction uniform after turning, be then charged into 500 milliliter three In the bottle of angle, every bottle of bent material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, in high-pressure sterilizing pot With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain, One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, treats Detain bottle after molding, continue to cultivate 72-80 hour；In the cultured Triangle-botde koji short time need not, can put Deposit in entering 4 DEG C of refrigerators, the resting period not can exceed that one week standby；

(2.3.3) bent box yeast inoculation cultivates (three grades of kinds)

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40 60 minutes, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with The gunnysack of sterilizing covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, rice-koji Mould spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness 1.5 2 centimetres, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material； Temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, bent material Molding, can turn over song；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry Cotton cloth, covers wet cotton cloth, and to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether Through 72-96 hour, the black spore of bent material was the most aging, can dry song or shine song；Dry deep closet temperature to bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life More than 3 months；

(3) aerated koji making cultivates (production yeast inoculation)

The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation, Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore Germinate, static gas wave refrigerator 6-8 hour；Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling, Control temperature between 30 35 DEG C；

Song expects that pond, after 12 hours cultivate, carries out turning over song for the first time, makes bent material loose, reduces flowing resistance, Keep normal temperature 30-35 DEG C；After continuing to cultivate 4-6 hour, carry out second time in time and turn over song；Incubation time arrives 24-28 hour；

(4) song is become to dry

Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.

The explanation of former strain and source:

Aspergillus oryzae (As3.800): main product saccharifying type enzyme activity, is brewing yellow rice wine saccharifying agent aspergillosis；

Yellow aspergillosis (As3.951): mainly produce neutral protease vigor, saccharifying enzymic activity；

Aspergillus niger (As3.324): main product saccharifying type enzyme activity, acid protease activity；

Strain derives from Beijing City Food Brewing Research Institute.

Selecting the Fructus Hordei Vulgaris of raw material, Semen Tritici aestivi, Semen sojae atricolor, Semen Pisi sativi is starter-making materials, be for growth provide good nitrogen source, The nutrition parts such as carbon source, promote that microorganism well grows and the formation of enzyme vigor.

Unit symbol ° B ' e shown in above-mentioned refers to: Baume concentration.

Claims (2)

1. a manufacture method for distillers yeast, the raw material of employing, by weight, and Fructus Hordei Vulgaris 3 parts, Semen Tritici aestivi 4 parts, Semen sojae atricolor 1.5 parts, Semen Pisi sativi 1.5 parts；The yeast inoculation used, according to the percentage by weight with raw material, aspergillus oryzae song box yeast inoculation 1 ‰, Aspergillus flavus song box yeast inoculation 1 ‰, aspergillus niger song box yeast inoculation 1 ‰；Specifically comprise the following steps that

(1) Feedstock treating: by Fructus Hordei Vulgaris, wheat crushing, according to each the standard being ground into 3-5 grain, as far as possible subtract Few powder (because Semen Tritici aestivi viscosity is big), it is 40-60 mesh that Semen sojae atricolor, Semen Pisi sativi are pulverized, and then, Semen sojae atricolor, Semen Pisi sativi is respectively put into Soaking 1-2 hour in 75-90 DEG C of hot water, Fructus Hordei Vulgaris, Semen Tritici aestivi are separately added into material moistening in the tap water of mass percent 40% 1-2 hour, then with the Semen sojae atricolor moistened, Bowl bean powder mix homogeneously, in the material steaming tank of 0.2-0.3MPa pressure steam Expecting 10-15 minute, discharging is cooled to 40 DEG C, i.e. can be accessed by yeast inoculation and enters to cultivate in the ventilation koji bed of sterilizing room as becoming bent；

(2) cultivation of bent box yeast inoculation:

(2.1) cultivation of aspergillus oryzae:

(2.1.1) slant tube spawn culture

Culture medium 8-9 ° of B ' e rice-koji juice or beerwort 100 milliliters, add 2.5 grams of agar, makes solid examination by well-established law Pipe slant medium；After aseptically accessing original seed, cultivate 72-80 hour in 28-30 DEG C of constant incubator ?；It is standby that cultured slant tube is positioned over preservation in refrigerator, and refrigerator temperature controls at 4-6 DEG C；

(2.1.2) triangular flask amplification culture

Take fresh wheat bran and add water about 85% by quality, make water suction uniform after turning, be then charged into 500 milliliters of triangles In bottle, every bottle of bent material thickness 1-1.5 centimetre, it is stoppered tampon, bottleneck kraft paper is wrapped up, in high-pressure sterilizing pot With 0.15-0.2MPa pressure sterilization 30-40 minute, take out cooling, aseptically access slant tube strain, One slant tube accesses in 20 bottles of triangular flasks, then puts in 28-30 DEG C of constant incubator, through 34 hours, and knot Detain bottle after cake, continue to cultivate 72-80 hour；4 DEG C need not be put in the cultured Triangle-botde koji short time Depositing standby in refrigerator, the time is less than one week；

(2.1.3) bent box yeast inoculation is cultivated

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, Become bent material；I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method be first by the alcoholic fermented product starter with After a small amount of bent material mix homogeneously, then being spread on bent material, after 2-3 time turns, being contained in bamboo basket, interior (temperature exists 30-32 DEG C), then cover with sterilized gunnysack, piled up through 7-8 hour in placing the deep closet of 28-30 DEG C, temperature Rising to 35-37 DEG C, aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, then Load song to close, bent material thickness 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, To ensure the humidity of song material；Indoor temperature should control at 28-30 DEG C, and after 10 hours, mycelia starts the most numerous Growing, temperature rises to 34-35 DEG C, bent material molding, can turn over song；Method is carefully by the song of molding with bamboo chip Carry out turn-over, turn over song and to change the most dry cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C, Promoting mycelial growth spore, altogether through 72-96 hour, the yellow spore of bent material is the most aging, can dry song or shine song； Drying deep closet temperature and can bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry room Inside save backup；

(2.2) cultivation of Aspergillus flavus

(2.2.1) slant tube spawn culture

Manufacturing of culture medium, takes 1000 milliliters of the Semen Glycines juice of 5-6 ° of B ' e, 0.6 gram of magnesium sulfate, soluble starch 20 grams, potassium dihydrogen phosphate 1 gram, 0.5 gram of ammonium sulfate, 20 grams of agar, load after dissolving in test tube, cotton beyond the Great Wall Plug, bottleneck outsourcing kraft paper, put into pressure cooker sterilizing 30-40 minute under 0.15-0.2MPa steam pressure, take out It is put into slant tube while hot, moves on in constant incubator after waiting test tube slant cooling, it is ensured that temperature 35-37 DEG C, permanent Temperature is cultivated 72-80 hour, to measure slant tube culture medium whether aseptic inoculation, is existed by the slant tube that sterilizing is good Aspergillus flavus test tube original seed, an original seed switching slant tube 20 is accessed under aseptic condition；Connecting original seed Test tube is positioned in the calorstat of 28-30 DEG C cultivation 72-80 hour, the slant tube bacterium that now should will grow It is standby that kind is put in preservation in 4 DEG C of refrigerators；

(2.2.2) the triangular flask alcoholic fermented product starter is cultivated

Take fresh wheat bran and add water 85%, make water suction uniform after turning, be then charged in 500 milliliters of triangular flasks, every bottle Thickness is 1-1.5 centimetre, is stoppered tampon, and bottleneck external kraft paper is wrapped up, with 0.15-0.2MPa in high-pressure sterilizing pot Pressure sterilization 30-40 minute, takes out cooling, aseptically accesses slant tube strain, a slant tube Access in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, after 34h, molding, detain bottle, continue training Support 72-80 hour；4 DEG C of refrigerators need not be put in the cultured Triangle-botde koji short time and deposit standby With, deposit and not can exceed that one week；

(2.2.3) bent box yeast inoculation is cultivated

Weigh 100 kilograms of fresh wheat bran, add water 80%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, inoculum concentration is the 0.3% of fresh wheat bran quality；Method is first by mixed with a small amount of bent material for the alcoholic fermented product starter After closing uniformly, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then uses Sterilized gunnysack covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35-37 DEG C, Aspergillus oryzae spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness Spend 1.5-2 centimetre, then on the shelf on song material, cover sterilized wet cotton cloth, to ensure the humidity of song material； Temperature temperature is at 28-30 DEG C, and after 10 hours, mycelia starts amount reproduction, and temperature rises to 34-35 DEG C, bent Expect molding, song can be turned over；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry Cotton cloth, cover wet cotton cloth, to ensure humidity, temperature at 30-36 DEG C, promote mycelial growth spore, Altogether through 72-96 hour, the yellow green spore of bent material is the most aging, can dry song or shine song；Dry deep closet temperature to bring up to 40-45 DEG C, alcoholic fermented product starter moisture, below 12%, bandages with plastic bag, puts dry indoor and saves backup, the shelf-life More than 3 months；

(2.3), the cultivation of Aspergillus niger

(2.3.1) slant tube spawn culture

Employing Czapek's medium: ammonium nitrate 3 grams, 0.5 gram of potassium chloride, 0.01 gram of ferrous sulfate, potassium dihydrogen phosphate 1 Gram, 0.5 gram of magnesium sulfate, sucrose 20 grams, 20 grams of agar, distilled water 1000 milliliters, load in test tube after dissolving and fill in Upper tampon, outsourcing kraft paper, put in pressure cooker sterilizing 30-40 under vapour pressure is with 0.15-0.2MPa pressure and divide Clock, takes out and is put into inclined-plane while hot, and cooling is placed in 35-37 DEG C of constant incubator cultivation 72 hours, to measure inclined-plane Examination medium sterilization is the most thorough；Inoculation, aseptically accesses test tube original seed slant tube good for sterilizing, One test tube original seed is transferred 20, and the slant tube inoculated puts into cultivation 72-80 in 28-30 DEG C of constant incubator Hour, it is standby that cold preservation in 4 DEG C of refrigerators put into by ripe test tube；(2.3.2) Triangle-botde koji is cultivated

Take fresh wheat bran and add water 80%, make water suction uniform after turning, be then charged in 500ml triangular flask, every bottle of song Material thickness is 1-1.5 centimetre, is stoppered tampon, wraps up with kraft paper, presses with 0.15-0.2MPa in high-pressure sterilizing pot Power sterilizing 30-40 minute, takes out cooling, aseptically accesses slant tube strain, and a slant tube connects Enter in 20 bottles of triangular flasks, then put in 28-30 DEG C of constant incubator, through 34 hours, after molding, detain bottle, continue Continuous cultivate 72-80 hour；In the cultured Triangle-botde koji short time need not, 4 DEG C of refrigerator internal memories can be put into Put, the resting period not can exceed that one week standby；

(2.3.3) bent box yeast inoculation is cultivated

Weigh 100 kilograms of fresh wheat bran, add water 85%, sieve after turning uniformly, make bent material loose without agglomerate, Normal pressure steaming in steaming stock pot, after all justifying vapour, then steams 40-60 minute, takes out spreading for cooling to 35 DEG C in sterilizing room, I.e. can be accessed by triangular flask kind, 10 Triangle-botde koji of inoculum concentration, method is first the alcoholic fermented product starter to be mixed all with a small amount of bent material After even, then being spread on bent material, after 2-3 time turns, will be contained in bamboo basket, temperature is at 30-32 DEG C, then with going out The gunnysack of bacterium covers, and piles up through 7-8 hour in placing the deep closet of 28-30 DEG C, and temperature has been raised to 35 DEG C, aspergillus oryzae Spore germinates, has bent perfume (or spice) i.e. to turn, and makes the most bent material uniformly, is then charged into bent conjunction, bent material thickness 1.5-2 Centimetre, then cover sterilized wet cotton cloth on the shelf on song material, to ensure the humidity of song material；Temperature exists 28-30 DEG C, after 10 hours, mycelia starts amount reproduction, and temperature has gone up 34-35 DEG C, and song expects molding, Song can be turned over；Method is carefully by the Qu Jinhang turn-over of molding with bamboo chip, turns over song and to change the most dry cotton cloth, Covering wet cotton cloth, to ensure humidity, temperature, at 30-36 DEG C, promotes mycelial growth spore, altogether through 72-96 Hour, the black spore of bent material is the most aging, can dry song or shine song；Dry deep closet temperature and bring up to 40-45 DEG C, bent Kind moisture, below 12%, bandages with plastic bag, puts dry indoor and save backup, more than 3 months shelf-life；

(3) aerated koji making is cultivated

The raw material that Fructus Hordei Vulgaris, Semen Tritici aestivi, Semen sojae atricolor and Semen Pisi sativi are constituted access aspergillus oryzae song box yeast inoculation, Aspergillus flavus song box yeast inoculation, Aspergillus niger song box yeast inoculation, after mix homogeneously, enters the Quchi that ventilates, and regulation temperature, at 30-35 DEG C, promotes strain spore Germinate, static gas wave refrigerator 6-8 hour；Now the bed of material begins to warm to 35-37 DEG C, should i.e. start blower ventilation cooling, Control temperature between 30-35 DEG C；

Song expects that pond, after 12 hours cultivate, carries out turning over song for the first time, makes bent material loose, reduces flowing resistance, Keep normal temperature 30-35 DEG C；After continuing to cultivate 4-6 hour, carry out second time in time and turn over song；Incubation time arrives 24-28 hour；

(4) song is become to dry

Cultured Cheng Qu is between 40-45 DEG C, through drying bed 24-36 hour,.

2. a distillers yeast, it is characterised in that: it is to use the manufacture method described in claim 1 to prepare.