CN105901692A - Preparation method of fermentation wheat grass nutrient solution - Google Patents

Preparation method of fermentation wheat grass nutrient solution Download PDF

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Publication number
CN105901692A
CN105901692A CN201610226557.6A CN201610226557A CN105901692A CN 105901692 A CN105901692 A CN 105901692A CN 201610226557 A CN201610226557 A CN 201610226557A CN 105901692 A CN105901692 A CN 105901692A
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CN
China
Prior art keywords
caulis
tritici aestivi
folium tritici
carbon dioxide
preparation
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Pending
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CN201610226557.6A
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Chinese (zh)
Inventor
纪小国
杨铭乾
赵祎
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Zhengzhou Guoshi Technology Co Ltd
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Zhengzhou Guoshi Technology Co Ltd
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Priority to CN201610226557.6A priority Critical patent/CN105901692A/en
Publication of CN105901692A publication Critical patent/CN105901692A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention belongs to the biotechnical field of foods, and concretely relates to a preparation method of a fermentation wheat grass nutrient solution. The preparation method comprises the following steps: 1, harvesting wheat grass having a distance of 1cm or above to the root when wheat seeds germinate and grow to 10-25cm, cutting the harvested wheat grass to form wheat grass segments with the length of 2-5cm, putting the wheat grass segments in a -20DEG C refrigerator, and freezing the wheat grass segments; 2, beating the wheat grass segments in nitrogen or carbon dioxide atmosphere in the presence of a color protection agent; 3, adding nutrient components, adjusting the pH value to 6.5-7.0, and carrying out mixed fermentation under slowly introduced nitrogen or carbon dioxide conditions; 4, after-ripening at 0-4DEG C, and adjusting the mouthfeel; and 5, filling, and continuously introducing nitrogen or carbon dioxide in the filling process. The mixed fermentation is carried out to degrade celluloses and proteins in the wheat grass and increase soluble dietary fibers, bioactive peptides and probiotics, so the problems of easy color change, nutrition loss and difficult preservation in traditional technologies are solved.

Description

A kind of preparation method of the Caulis et Folium Tritici aestivi nutritional solution that ferments
Technical field
The invention belongs to technical field of food biotechnology, be specifically related to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments.
Background technology
Caulis et Folium Tritici aestivi is Gramineae plant, is Roegneria C. Koch (Agropyron).The traditional Chinese medical science is " wheat seedling " also known as Caulis et Folium Tritici aestivi, has the effect of " main disappear alcoholism, sudden high fever, jaundice due to immoderate drinking mesh infantile malnutrition ".In its growth course, aerial parts stem and leaf cell constantly has the synthesis of protein, for building new cell tissue and the needs of organ, this partially protein is referred to as leaf protein (Leaves Protein Concentrates is called for short LPC), they belong to functional protein class, are also the reproducible protein resources of a kind of maximum.Leaf protein is a class heteropolymer matter, about contains 18 kinds of aminoacid, contains 8 kinds of aminoacid necessary to human body.The leaf protein extracted from greenery is of high nutritive value, eating effect is good, also contains chlorophyll, aminoacid, vitamin, mineral and ferment, has very big Development volue.Caulis et Folium Tritici aestivi tender leaf can be squeezed the juice or dry pulverizing.But crude plant contains a large amount of celluloses, it is difficult to digest for people.
The main method that Caulis et Folium Tritici aestivi is extracted processing at present mainly has salting out method, centrifugal separation, crystallization and recrystallization method, organic solvent precipitation method, heating (containing progressively and quickly heating), biologic enzymolysis method etc..The Caulis et Folium Tritici aestivi product that at present general production technology produces there is also variety of problems, as owing to greenery existing non-saturated fatty acid, and long-time heating in air and water, effective lysine can be caused to be lost, protein denaturation, thus make digestibility decline;Dried Caulis et Folium Tritici aestivi leaf protein dissolubility is extremely low, and digestibility and biological value are far away from the animal proteinum such as milk, egg, it is impossible to be used in the food systems such as milk, beverage, confection as functional components;Major Wheat grass product mostly is bottle green the most in the market, and has herbaceous taste, and these all limit its application in food.
Summary of the invention
The above-mentioned digestibility that it is an object of the invention to overcome prior art to exist is low, end product functional characteristic poor and there is the defects such as herbaceous taste, it is provided that the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments.
The preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments of the present invention, comprises the steps:
(1) when wheat seed germinating grows to 10~25 cm, the Caulis et Folium Tritici aestivi of harvesting root 1 more than cm, Caulis et Folium Tritici aestivi is cut into 2~5 cm length, puts into~20 DEG C of refrigerators freeze;
(2) per kilogram Caulis et Folium Tritici aestivi adds 3~7 kilograms of vitamin Cs (0.1%) and cysteine (0.1%) mixing colour-keeping liquid, pulls an oar under being passed through nitrogen or carbon dioxide conditions;
(3) in Semen Tritici aestivi straw pulp, high fructose syrup (10% is added, in terms of Caulis et Folium Tritici aestivi weight in wet base) and peptone (10%, in terms of Caulis et Folium Tritici aestivi weight in wet base), regulation pH 6.5~7.0, then in bacterium solution living bacteria count 3:1~2:1~2 ratio inoculation yeast bacterium, bacillus bifidus, BF839 bacteroides fragilis, seed liquor total amount is the 1% of Semen Tritici aestivi straw pulp, and ferment under the conditions of 37 DEG C 24~72 h;Sweat is slowly introducing nitrogen or carbon dioxide;
(4) under the conditions of inserting 0~4 DEG C, after-ripening 24~60 h, regulates mouthfeel afterwards;
(5) fill, continues in pouring process to be passed through nitrogen or carbon dioxide, and fill terminates i.e. to obtain the Caulis et Folium Tritici aestivi nutritional solution that ferments.
In described step (2), per kilogram Caulis et Folium Tritici aestivi is preferably added to 3~5 kilograms of mixing colour-keeping liquid.
In described step (2), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/kg Caulis et Folium Tritici aestivi.
In described step (3), described mixed vaccine preferred bacterium solution living bacteria count is than for yeast, bacillus bifidus, BF839 bacteroides fragilis=3:2:2.
In described step (3), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/kg Caulis et Folium Tritici aestivi.
Regulation pH value in described step (3), can regulate with the NaOH solution that concentration is 2 M.
In described step (3), under the conditions of 37 DEG C, fermentation is preferably 42 h.
Under the conditions of inserting 0~4 DEG C in described step (4), after-ripening is preferably 36 h.
In described step (5), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/kg Caulis et Folium Tritici aestivi.
Compared with prior art, the invention have the advantages that
The present invention uses biological fermentation process to be prepared for Caulis et Folium Tritici aestivi nutritional solution, whole preparation process is in middle low temperature environment, effectively save original bioactive ingredients in Caulis et Folium Tritici aestivi, prevent the brown stain of Caulis et Folium Tritici aestivi, and the cellulose in Caulis et Folium Tritici aestivi, albumen is degraded by mixed fungus fermentation, adding water soluble dietary fiber and biologically active peptide, obtained Caulis et Folium Tritici aestivi nutritional solution contains abundant nutrient and probiotic bacteria, solves the problems such as easy variable color in traditional handicraft, nutritive loss, preservation difficulty.
Detailed description of the invention
Some embodiments of the present invention presented below, to help being further appreciated by the present invention.
Embodiment 1
The first step: when wheat seed germinating is grown to 15 cm, the Caulis et Folium Tritici aestivi of harvesting more than root 1cm, Caulis et Folium Tritici aestivi is cut into 5cm length, puts into-20 DEG C of refrigerators and freeze;
Second step: per kilogram Caulis et Folium Tritici aestivi adds three kilograms of vitamin Cs (0.1%) and cysteine (0.1%) mixed liquor, pulls an oar under being passed through nitrogen or carbon dioxide conditions;
3rd step: add high fructose syrup (10% in Semen Tritici aestivi straw pulp, in terms of Caulis et Folium Tritici aestivi weight in wet base) and peptone (10%, in terms of Caulis et Folium Tritici aestivi weight in wet base), regulation pH 6.5~7.0, then in bacterium solution viable count 3:2:2 ratio inoculation yeast bacterium, bacillus bifidus, BF839 bacteroides fragilis, seed liquor total amount is the 1% of Semen Tritici aestivi straw pulp, and ferment under the conditions of 37 DEG C 42 h, is passed through nitrogen with the speed of 0. 1L/min/kg Caulis et Folium Tritici aestivi in sweat;
4th step: after-ripening 36 h under the conditions of inserting 0~4 DEG C, regulates mouthfeel afterwards;
5th step: quantitative filling, is passed through nitrogen with the speed of 0.3L/min/kg Caulis et Folium Tritici aestivi in pouring process, and fill terminates i.e. to obtain the Caulis et Folium Tritici aestivi nutritional solution that ferments.
Embodiment 2
The first step: when wheat seed germinating is grown to 20 cm, the Caulis et Folium Tritici aestivi of harvesting more than root 1cm;
Second step: Caulis et Folium Tritici aestivi is cut into 5cm length, put into-20 DEG C of refrigerators to freeze, per kilogram Caulis et Folium Tritici aestivi adds three kilograms of vitamin Cs (0.1%) and cysteine (0.1%) mixed liquor, pulls an oar under being passed through carbon dioxide conditions, and carbon dioxide is passed through speed and is: 0.1L/min/kg Caulis et Folium Tritici aestivi;
3rd step: add high fructose syrup (10% in Semen Tritici aestivi straw pulp, in terms of Caulis et Folium Tritici aestivi weight in wet base) and peptone (12%, in terms of Caulis et Folium Tritici aestivi weight in wet base), regulation pH6.5~7.0, then in bacterium solution viable count 3:2:3 ratio inoculation yeast bacterium, bacillus bifidus, BF839 bacteroides fragilis, seed liquor total amount is the 1% of Semen Tritici aestivi straw pulp, and ferment under the conditions of 37 DEG C 60 h;Sweat is passed through nitrogen with the speed of 0. 3L/min/kg Caulis et Folium Tritici aestivi;
4th step: after-ripening 60 h under the conditions of inserting 0~4 DEG C, regulates mouthfeel afterwards;
5th step: quantitative filling, with the speed carbon dioxide of 0. 15L/min/kg Caulis et Folium Tritici aestivi in pouring process, fill terminates i.e. to obtain the Caulis et Folium Tritici aestivi nutritional solution that ferments.

Claims (6)

1. ferment the preparation method of Caulis et Folium Tritici aestivi nutritional solution, it is characterized in that, comprise the following steps:
(1) when wheat seed germinating grows to 10~25 cm, the Caulis et Folium Tritici aestivi of harvesting root 1 more than cm, Caulis et Folium Tritici aestivi is cut into 2~5 cm length, puts into~20 DEG C of refrigerators freeze;
(2) per kilogram Caulis et Folium Tritici aestivi adds 3~7 kilograms of vitamin Cs (0.1%) and cysteine (0.1%) mixing colour-keeping liquid, pulls an oar under being passed through nitrogen or carbon dioxide conditions;
(3) in Semen Tritici aestivi straw pulp, high fructose syrup (10% is added, in terms of Caulis et Folium Tritici aestivi weight in wet base) and peptone (10%, in terms of Caulis et Folium Tritici aestivi weight in wet base), regulation pH 6.5~7.0, then inoculation yeast bacterium, bacillus bifidus, BF839 bacteroides fragilis, seed liquor total amount is the 1% of Semen Tritici aestivi straw pulp, and ferment under the conditions of 37 DEG C 24~72 h, is slowly introducing nitrogen or carbon dioxide in sweat;
(4) under the conditions of inserting 0~4 DEG C, after-ripening 24~60 h, regulates mouthfeel afterwards;
(5) fill, continues in pouring process to be passed through nitrogen or carbon dioxide, and fill terminates i.e. to obtain the Caulis et Folium Tritici aestivi nutritional solution that ferments.
2. according to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments described in claim 1, it is characterized in that, in described step (1), Caulis et Folium Tritici aestivi is cut into 2~5 cm length, puts into-20 DEG C of refrigerators and freeze.
3. according to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments described in claim 1, it is characterized in that, in described step (2), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/Kg Caulis et Folium Tritici aestivi.
4. according to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments described in claim 1, it is characterized in that, in described step (3), in bacterium solution living bacteria count 3:1~2:1~2 ratio inoculation yeast bacterium, bacillus bifidus, BF839 bacteroides fragilis.
5. according to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments described in claim 1, it is characterized in that, in described step (3), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/kg Caulis et Folium Tritici aestivi.
6. according to the preparation method of a kind of Caulis et Folium Tritici aestivi nutritional solution that ferments described in claim 1, it is characterized in that, in described step (5), nitrogen or carbon dioxide ventilation are 0.01-0.3L/min/kg Caulis et Folium Tritici aestivi.
CN201610226557.6A 2016-04-13 2016-04-13 Preparation method of fermentation wheat grass nutrient solution Pending CN105901692A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106819722A (en) * 2016-12-19 2017-06-13 蚌埠鲲鹏食品饮料有限公司 A kind of fermented health-care agropyron beverage
CN113142582A (en) * 2021-03-10 2021-07-23 江南大学 Dendrobium officinale instant powder and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103300293A (en) * 2013-07-02 2013-09-18 四川农业大学 Processing method of wheat seedings as well as instant wheat seeding powder and wheat seeding fiber powder prepared from same
CN105166907A (en) * 2015-10-10 2015-12-23 宁波海通食品科技有限公司 Method for preparing barley seedling ferment through quick fermentation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103300293A (en) * 2013-07-02 2013-09-18 四川农业大学 Processing method of wheat seedings as well as instant wheat seeding powder and wheat seeding fiber powder prepared from same
CN105166907A (en) * 2015-10-10 2015-12-23 宁波海通食品科技有限公司 Method for preparing barley seedling ferment through quick fermentation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张华江主编: "《食品添加剂原理与应用》", 30 September 2014, 中国农业出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106819722A (en) * 2016-12-19 2017-06-13 蚌埠鲲鹏食品饮料有限公司 A kind of fermented health-care agropyron beverage
CN113142582A (en) * 2021-03-10 2021-07-23 江南大学 Dendrobium officinale instant powder and preparation method thereof

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Application publication date: 20160831