CN105901020A - Microbial pesticide with bacillus subtilis and method for preparing microbial pesticide - Google Patents
Microbial pesticide with bacillus subtilis and method for preparing microbial pesticide Download PDFInfo
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- CN105901020A CN105901020A CN201610276234.8A CN201610276234A CN105901020A CN 105901020 A CN105901020 A CN 105901020A CN 201610276234 A CN201610276234 A CN 201610276234A CN 105901020 A CN105901020 A CN 105901020A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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Abstract
The invention provides a microbial pesticide with bacillus subtilis. The microbial pesticide comprises, by weight, 11-19 parts of propargite, 7-15 parts of solvents and complex solubilizers, 6-14 parts of emulsifiers, 3-5 parts of stabilizers, 2-4 parts of synergists, 13-21 parts of water, 2-4 parts of momordica charantia extract, 1-3 parts of fructus xanthii suspension liquid, 1-3 parts of protein and 2-6 parts of the bacillus subtilis. The invention further provides a method for preparing the microbial pesticide with the bacillus subtilis. The microbial pesticide and the method have the advantages that excellent effects of killing mite eggs, larvas, nymphs and adult insects can be realized by the microbial pesticide; good effects of killing injurious insects such as moths and injurious insects with shells can be realized; effects of inhibiting and resisting bacteria further can be realized by the microbial pesticide, and the microbial pesticide is long in bacterium inhibition period.
Description
Technical field
The invention belongs to preparation technique of pesticide field, be specifically related to a kind of microbial pesticide containing bacillus subtilis and
Its preparation method.
Background technology
The definition of pesticide broad sense refer to for prevent, eliminate or control endanger agricultural, the disease of forestry, worm, grass and other
Harmful organism and on purpose regulate, control, affect plant and harmful organism metabolism, grow, grow, the chemistry of reproductive process
Synthesize or derive from a kind of material or the mixing of several material that biology, other natural products and Applied Biotechnology produce
Thing and preparation thereof.Refer in the narrow sense in agricultural production, for ensureing, promote plant and the growth of crops, the parasite killing used,
Sterilization, kill harmful animal (or weeds) one class medicine be referred to as.Refer in particular to agriculturally for preventing and treating disease pest and regulation plant
The medicaments such as growth, weeding.
Pesticide is divided into chemical pesticide, biological pesticide, transgenic pesticide and mineral sources pesticide.Biological pesticide is divided into plant source agriculture
Medicine, animal pesticide and microbial pesticide.Microbial pesticide refer to by microorganisms such as antibacterial, fungus, actinomycetes, viruses and
The pesticide that its metabolite is processed into.
Microbial pesticide is the NPD projects of 21 century pesticide industry, represents the direction of plant protection, its maximum excellent
Gesture is to overcome chemical pesticide to the pollution of ecological environment and to reduce at agricultural byproducts Pesticide Residues, pushes away in demonstration simultaneously
During wide microbial pesticide application, the quality of agricultural byproducts and price will significantly rise, and advantageously facilitate rural area warp
Ji increases and increasing peasant income, and social benefit is inestimable.
But at present, widely used microbial pesticide is the most less desirable to the prevention effect of insect, it is impossible to effectively
Kill disease and insect.Such as, existing microbial pesticide is in terms of preventing and treating acarid, if can only be to acarid ovum, larva demodicid mite and adult
In certain stage of growth insect killing effect good, can not reach to kill the effect of acarid comprehensively；And for example, existing microbial pesticide
Acarid can only be killed, it is impossible to other moth classes, shell class pest are effectively killed, or other moth class, shell class pest are gone out
Kill and have little effect；For another example, insect can only effectively be prevented and treated by existing microbial pesticide, but to pathogenic bacteria without killing effect,
Can not control the sickness rate of phytopathogen, antibacterial, antibacterial effect is poor.
In summary, prior art there will naturally be inconvenience and defect in actual use, it is therefore necessary to improved.
Summary of the invention
For solving technical problem present in prior art, the present invention provides a kind of microbial source containing bacillus subtilis
Pesticide and preparation method thereof, to realize following goal of the invention:
If 1, the present invention is splendid to acarid ovum, larva demodicid mite and adult insect killing effect；
2, the present invention is good to insect insect killing effects such as moth class, shell classes；
3, the present invention can also be antibacterial, antibacterial, and antibacterial timeliness is long.
For solving above-mentioned technical problem, the technical scheme that the present invention takes is as follows:
A kind of microbial pesticide containing bacillus subtilis, described microbial pesticide includes following components: propargite, solvent
And cosolvent, emulsifying agent, stabilizer, synergist, water, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein, bacillus subtilis.
The following is and the further of technique scheme is improved:
The component of described microbial pesticide, in parts by weight, content is:
Propargite 11～19 parts, solvent and cosolvent 7～15 parts, emulsifying agent 6～14 parts, stabilizer 3～5 parts, synergist 2～4
Part, water 13～21 parts, Fructus Momordicae charantiae extract 2～4 parts, Fructus Xanthii suspension 1～3 parts, protein 1～3 parts, bacillus subtilis 2
～6 parts.
The component of described microbial pesticide, in parts by weight, content is:
Propargite 15 parts, solvent and cosolvent 11 parts, emulsifying agent 10 parts, stabilizer 4 parts, synergist 3 parts, 17 parts of water, Fructus Momordicae charantiae carry
Take thing 3 parts, Fructus Xanthii suspension 2 parts, 2 parts of protein, bacillus subtilis 4 parts.
Described solvent and cosolvent are n-butyl alcohol.
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether.
Described stabilizer is tetramethylolmethane.
Described synergist is potentiation fat.
Described Fructus Momordicae charantiae extract includes: Charantin, Charantin and Fructus Momordicae charantiae fat eliminating element；Described Charantin content is 35%, bitter
Melon cellulose content is 30%, and Fructus Momordicae charantiae fat eliminating cellulose content is 30%.
The preparation method of a kind of microbial pesticide containing bacillus subtilis, comprises the following steps:
(1) raw material prepares: take propargite, n-butyl alcohol, benzylphenol polyoxyethylene according to the weight ratio of above-described embodiment 1～3
Ether, tetramethylolmethane, potentiation fat, water, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein and bacillus subtilis；
(2) mixing: by the propargite of taken weight portion, n-butyl alcohol, Ben-zylphenol Polyoxyethyl Ether, tetramethylolmethane, increasing in step (1)
Effect fat, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein, dead leaf bacillus cereus are put in shears still, open high-shear emulsifying
Machine, after high-shear emulsion machine works 10 minutes, raw material shear-mixed is uniform, obtains the first mixture；
(3) ball milling: the first mixture of gained in step (2) is put into ball milling 20 minutes in ball mill, to without visible bulk
Grain；
(4) material that ball milling is good is added grinding machine for grinding so that it is particle diameter is 4 microns～5 microns；
(5) microbial pesticide of the present invention is i.e. obtained.
The extracting method of described Fructus Momordicae charantiae extract comprises the following steps:
(1) taking Fructus Momordicae charantiae and remove seed, pulverize, after soaking with 14 volume times amount 80% ethanol waters, reflux, extract, is for several times, extracts every time
5h, filters, and obtains ethanol extract and filtering residue, merges ethanol extract, stands 48h, filters off precipitation, and recycling design obtains crude extract；
(2) water-soluble crude extract, upper macroporous adsorptive resins, after washing desaccharide, with 95% ethanol water eluting, collect ethanol and wash
De-liquid, recycling design obtains water-soluble extract；
(3) water-soluble extract, successively with 15 volume times amount dichloromethane, ethyl acetate, n-butanol extraction；
(4) concentrating remaining aqueous phase after extraction, being washed till concentrate with methanol can not be dissolved again, obtains final Fructus Momordicae charantiae extract.
Compared with prior art, the invention have the benefit that
If 1, the present invention reaches 98.5%～100% to acarid ovum, larva demodicid mite and adult eradicative rate, insecticidal effect is splendid；
2, the present invention is to insect eradicative rates such as moth class, shell classes up to 97% ~ 99%, and insect killing effect is good；
3, the present invention can suppress pathogenic bacteria to increase, and pathogenic bacteria corrodes in opposing, and sickness rate controls 2% ~ 6%, and antibacterial timeliness is up to 6
Month.
Detailed description of the invention
Below by way of specific embodiment, the present invention is described, but the present invention is not merely defined in these embodiments.
The embodiment 1 one kinds microbial pesticide containing bacillus subtilis
Described microbial pesticide includes the component of following weight portion:
Propargite 11 parts, solvent and cosolvent 7 parts, emulsifying agent 6 parts, stabilizer 3 parts, synergist 2 parts, 13 parts of water, Fructus Momordicae charantiae extract
Thing 2 parts, Fructus Xanthii suspension 1 part, 1 part of protein, bacillus subtilis 2 parts；
Described solvent and cosolvent are n-butyl alcohol；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described stabilizer is tetramethylolmethane；
Described synergist is potentiation fat；
Described Fructus Momordicae charantiae extract includes: Charantin, Charantin and Fructus Momordicae charantiae fat eliminating element；Described Charantin content is 35%, Charantin
Content is 30%, Fructus Momordicae charantiae fat eliminating cellulose content is 30%；
Described bacillus subtilis uses commercially available mode to obtain, and described bacillus subtilis living bacteria count is: 9.0 ×
109cfu/mL~10.0×109cfu/mL。
The embodiment 2 one kinds microbial pesticide containing bacillus subtilis
Described microbial pesticide includes the component of following weight portion:
Propargite 15 parts, solvent and cosolvent 11 parts, emulsifying agent 10 parts, stabilizer 4 parts, synergist 3 parts, 17 parts of water, Fructus Momordicae charantiae carry
Take thing 3 parts, Fructus Xanthii suspension 2 parts, 2 parts of protein, bacillus subtilis 4 parts；
Described solvent and cosolvent are n-butyl alcohol；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described stabilizer is tetramethylolmethane；
Described synergist is potentiation fat；
Described Fructus Momordicae charantiae extract includes: Charantin, Charantin and Fructus Momordicae charantiae fat eliminating element；Described Charantin content is 35%, Charantin
Content is 30%, Fructus Momordicae charantiae fat eliminating cellulose content is 30%；
Described bacillus subtilis uses commercially available mode to obtain, and described bacillus subtilis living bacteria count is: 9.0 ×
109cfu/mL~10.0×109cfu/mL。
The embodiment 3 one kinds microbial pesticide containing bacillus subtilis
Described microbial pesticide includes the component of following weight portion:
Propargite 19 parts, solvent and cosolvent 15 parts, emulsifying agent 14 parts, stabilizer 5 parts, synergist 4 parts, 21 parts of water, Fructus Momordicae charantiae carry
Take thing 4 parts, Fructus Xanthii suspension 3 parts, 3 parts of protein, bacillus subtilis 6 parts；
Described solvent and cosolvent are n-butyl alcohol；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described stabilizer is tetramethylolmethane；
Described synergist is potentiation fat；
Described Fructus Momordicae charantiae extract includes: Charantin, Charantin and Fructus Momordicae charantiae fat eliminating element；Described Charantin content is 35%, Charantin
Content is 30%, Fructus Momordicae charantiae fat eliminating cellulose content is 30%.
Described bacillus subtilis uses commercially available mode to obtain, and described bacillus subtilis living bacteria count is: 9.0 ×
109cfu/mL~10.0×109cfu/mL。
The preparation method of the embodiment 4 one kinds microbial pesticide containing bacillus subtilis
The preparation method of a kind of microbial pesticide containing bacillus subtilis comprises the following steps:
(1) raw material prepares: take propargite, n-butyl alcohol, benzylphenol polyoxyethylene according to the weight ratio of above-described embodiment 1～3
Ether, tetramethylolmethane, potentiation fat, water, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein and bacillus subtilis；
(2) mixing: by the propargite of taken weight portion, n-butyl alcohol, Ben-zylphenol Polyoxyethyl Ether, tetramethylolmethane, increasing in step (1)
Effect fat, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein, dead leaf bacillus cereus are put in shears still, open high-shear emulsifying
Machine, after high-shear emulsion machine works 10 minutes, raw material shear-mixed is uniform, obtains the first mixture；
(3) ball milling: the first mixture of gained in step (2) is put into ball milling 20 minutes in ball mill, to without visible bulk
Grain；
(4) material that ball milling is good is added grinding machine for grinding so that it is particle diameter is 4 microns～5 microns；
(5) microbial pesticide of the present invention is i.e. obtained.
The extracting method of described Fructus Momordicae charantiae extract comprises the following steps:
(1) taking Fructus Momordicae charantiae and remove seed, pulverize, after soaking with 14 volume times amount 80% ethanol waters, reflux, extract, is for several times, extracts every time
5h, filters, and obtains ethanol extract and filtering residue, merges ethanol extract, stands 48h, filters off precipitation, and recycling design obtains crude extract；
(2) water-soluble crude extract, upper macroporous adsorptive resins, after washing desaccharide, with 95% ethanol water eluting, collect ethanol and wash
De-liquid, recycling design obtains extract；
(3) water-soluble extract, successively with 15 volume times amount dichloromethane, ethyl acetate, n-butanol extraction；
(4) concentrating remaining aqueous phase after extraction, being washed till concentrate with methanol can not be dissolved again, obtains final extract.
Percent in above-described embodiment, in addition to specified otherwise, for mass percent.
Result detects:
(1) if to acarid ovum, larva demodicid mite, the insect killing effect of adult
If acarid ovum, larva demodicid mite, each 600 of adult, if taking acarid ovum at random, larva demodicid mite, each 200 of adult are 1 group, it is divided into
Experimental group 1, experimental group 2, experimental group 3, be respectively put into experimental group 1, experimental group 2, experimental group 3 in airtight glass bell jar；
If the acarid ovum of experimental group 1, larva demodicid mite, adult use the microbial pesticide of embodiment 1 to spray；Experimental group 2
If acarid ovum, larva demodicid mite, adult use the microbial pesticide of embodiment 2 to spray；The acarid ovum of experimental group 3, larva,
If demodicid mite, adult use the microbial pesticide of embodiment 3 to spray；
Spraying continues to terminate after 10 seconds, if record acarid ovum, larva demodicid mite, adult interior killing in 5 minutes the results are shown in Table 1；
If table 1 acarid ovum, larva demodicid mite, the dead number of elements of adult, mortality statistics
From table 1, if the present invention is extremely effective to the insect killing effect of acarid ovum, larva demodicid mite and adult, acarid ovum is sprayed
After mist, dead 198～200 of acarid ovum, mortality rate is 99%～100%；After larva is sprayed, dead larvae 198～200
Only, mortality rate is 99%～100%；If after spraying demodicid mite, if dead 197～198 of demodicid mite, mortality rate is 98.5%～99%；
After spraying adult, dead 197～199 of adult, mortality rate is 98.5%～99.5%.
(2) insect killing effect to insects such as moth class, shell classes
Choose diamondback moth, leaf roller, each 600 of big gourdshaped weevil first, phyllotreta striolata, take diamondback moth, leaf roller, big gourdshaped weevil at random
Each 200 of first, phyllotreta striolata is 1 group, is divided into experimental group 1, experimental group 2, experimental group 3, by experimental group 1, experimental group 2, experiment
Group 3 is respectively put in airtight glass bell jar；
The diamondback moth of experimental group 1, leaf roller, big gourdshaped weevil first, phyllotreta striolata use the microbial pesticide of embodiment 1 to spray
Mist；The diamondback moth of experimental group 2, leaf roller, big gourdshaped weevil first, phyllotreta striolata use the microbial pesticide of embodiment 2 to spray
Mist；The diamondback moth of experimental group 3, leaf roller, big gourdshaped weevil first, phyllotreta striolata use the microbial pesticide of embodiment 3 to spray
Mist；
Spraying continues to terminate after 20 seconds, and what record diamondback moth, leaf roller, big gourdshaped weevil first, phyllotreta striolata 10 minutes were interior kills knot
Fruit is shown in Table 2；
Table 2 diamondback moth, leaf roller, the dead number of elements of big gourdshaped weevil first, phyllotreta striolata, mortality statistics
From table 2, the present invention is good to diamondback moth, leaf roller, big gourdshaped weevil first, phyllotreta striolata insect killing effect, enters diamondback moth
After row spraying, dead 194～197 of diamondback moth, mortality rate is 97%～98.5%；Dead 195～198 of leaf roller, mortality rate
It is 97.5%～99%；Dead 195～196 of big gourdshaped weevil first, mortality rate is 97.5%～98.5%；Phyllotreta striolata dead 194～
197, mortality rate is 97%～98.5%.Other moth classes and shell class pest are also tested by the present invention, and insect killing effect is splendid,
List the most one by one at this.
(3) antibacterial, antibacterial effect
Choose 200, the Cotton Gossypii being uninfected by root nodule bacteria and anthrax bacillus, be randomly divided into 4 groups, respectively matched group, experimental group 1, reality
Test group 2 and experimental group 3, often 50 Cotton Gossypiis of group；
Experimental group 1 is sprayed the microbial pesticide of embodiment 1, experimental group 2 is sprayed the microbial pesticide of embodiment 2, right
Experimental group 3 sprays the microbial pesticide of embodiment 3, and matched group does not carry out any pesticide spraying, and spraying time continues 30 seconds,
From spraying, to matched group, experimental group 1, experimental group 2 and the root nodule bacteria of experimental group 3 and anthrax bacillus sickness rate when the 7th day
Carry out statistics and be shown in Table 3；
The control situation of Cotton Gossypii sickness rate is added up by 3 microbial pesticides of table
From table 3, the present invention is antibacterial, antibacterial effect is good, it is possible to effectively suppression root nodule bacteria and the sickness rate of anthrax bacillus, to cotton
Flower sprays after this microbial pesticide, and the sickness rate of root nodule bacteria controls 2%～6%, the sickness rate of anthrax bacillus control 2%～
6%.Other pathogenic bacterias are also tested by the present invention, and sickness rate is the most extremely low, and bacteriostasis antibiosis effect is good, lists the most one by one at this.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (10)
1. the microbial pesticide containing bacillus subtilis, it is characterised in that: described microbial pesticide includes following group
Point:
Propargite, solvent and cosolvent, emulsifying agent, stabilizer, synergist, water, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, albumen
Matter, bacillus subtilis.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described micro-life
The component of thing source pesticide, in parts by weight, content is:
Propargite 11～19 parts, solvent and cosolvent 7～15 parts, emulsifying agent 6～14 parts, stabilizer 3～5 parts, synergist 2～4
Part, water 13～21 parts, Fructus Momordicae charantiae extract 2～4 parts, Fructus Xanthii suspension 1～3 parts, protein 1～3 parts, bacillus subtilis 2
～6 parts.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described micro-life
The component of thing source pesticide, in parts by weight, content is:
Propargite 15 parts, solvent and cosolvent 11 parts, emulsifying agent 10 parts, stabilizer 4 parts, synergist 3 parts, 17 parts of water, Fructus Momordicae charantiae carry
Take thing 3 parts, Fructus Xanthii suspension 2 parts, 2 parts of protein, bacillus subtilis 4 parts.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described solvent
And cosolvent is n-butyl alcohol.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described emulsifying
Agent is Ben-zylphenol Polyoxyethyl Ether.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described stable
Agent is tetramethylolmethane.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described potentiation
Agent is potentiation fat.
A kind of microbial pesticide containing bacillus subtilis the most according to claim 1, it is characterised in that: described hardship
Melon extract includes: Charantin, Charantin and Fructus Momordicae charantiae fat eliminating element；Described Charantin content is 35%, and Charantin content is 30%, bitter
Melon fat eliminating cellulose content is 30%.
9. the preparation method of the microbial pesticide containing bacillus subtilis, it is characterised in that: comprise the following steps:
(1) raw material prepares: take propargite, n-butyl alcohol, benzylphenol polyoxyethylene according to the weight ratio of above-described embodiment 1～3
Ether, tetramethylolmethane, potentiation fat, water, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein and bacillus subtilis；
(2) mixing: by the propargite of taken weight portion, n-butyl alcohol, Ben-zylphenol Polyoxyethyl Ether, tetramethylolmethane, increasing in step (1)
Effect fat, Fructus Momordicae charantiae extract, Fructus Xanthii suspension, protein, dead leaf bacillus cereus are put in shears still, open high-shear emulsifying
Machine, after high-shear emulsion machine works 10 minutes, raw material shear-mixed is uniform, obtains the first mixture；
(3) ball milling: the first mixture of gained in step (2) is put into ball milling 20 minutes in ball mill, to without visible bulk
Grain；
(4) material that ball milling is good is added grinding machine for grinding so that it is particle diameter is 4 microns～5 microns；
(5) microbial pesticide of the present invention is i.e. obtained.
The preparation method of a kind of microbial pesticide containing bacillus subtilis the most according to claim 9, its feature exists
In:
The extracting method of described Fructus Momordicae charantiae extract comprises the following steps:
(1) taking Fructus Momordicae charantiae and remove seed, pulverize, after soaking with 14 volume times amount 80% ethanol waters, reflux, extract, is for several times, extracts every time
5h, filters, and obtains ethanol extract and filtering residue, merges ethanol extract, stands 48h, filters off precipitation, and recycling design obtains crude extract；
(2) water-soluble crude extract, upper macroporous adsorptive resins, after washing desaccharide, with 95% ethanol water eluting, collect ethanol and wash
De-liquid, recycling design obtains water-soluble extract；
(3) water-soluble extract, successively with 15 volume times amount dichloromethane, ethyl acetate, n-butanol extraction；
(4) concentrating remaining aqueous phase after extraction, being washed till concentrate with methanol can not be dissolved again, obtains final Fructus Momordicae charantiae extract.
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| CN115399343A (en) * | 2022-10-31 | 2022-11-29 | 山东睿鹰制药集团有限公司 | Plant composite antibacterial agent containing peony extract and preparation method and application thereof |
| CN115399343B (en) * | 2022-10-31 | 2023-01-24 | 山东睿鹰制药集团有限公司 | Plant composite antibacterial agent containing peony extract and preparation method and application thereof |
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