CN105886411A - Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain - Google Patents
Culture medium and culture method for inducing morphological transformation of Paecilomyces hepiali strain SH-1, and strain Download PDFInfo
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Abstract
The invention provides a culture medium and culture method for inducing morphological transformation of a Paecilomyces hepiali strain SH-1, and a strain, relating to the technical field of strain culture. Every 100ml of the liquid culture medium is mainly prepared from 2-5g of carbon source, 20-27g of nitrogen source (comprising eggs), 0.05-0.31g of inorganic salt (selected from one or more of magnesium sulfate and dipotassium hydrogen phosphate), and the balance of water. The culture medium is simple in components, and can be used for culturing the recognizable Paecilomyces hepiali strain with the same species as the Paecilomyces hepiali strain SH-1. The method provided by the invention is convenient to operate, has low requirements for environmental conditions, and is easy to implement. The strain provided by the invention is pink, has high recognition degree, and is easy to select.
Description
Technical field
The present invention relates to strain culturing technical field, induce bat in particular to one
Culture medium, the method for cultivation and the bacterial strain of moth Paecilomyces varioti bacterial strain SH-1 Morphological Transitions.
Background technology
Cordyceps sinensis is that aweto colonizes in the entomogenous fungi formed on Hepialus insect larva again
Zoarium, is the traditional rare traditional Chinese medicine of China, and the Pharmacopoeia of the People's Republic of China records its merit
Effect for " invigorate the lung and the kidney, hemostasis and phlegm.Breathe heavily for chronic cough void, labor coughs hemoptysis, impotence and seminal emission,
Soreness of waist and knee joint ".Sweet warm in nature, the mild in medicine property of Cordyceps sinensis taste and, temperature and the driest, mend and the most stagnant,
Than the traditional tonic medicine medical value the most widely of other kind and without any side effects.
Aweto is again Paecilomyces hepiali chen, the mycelium composition of its artificial culture, contains
Measure closely similar with natural cordyceps bacterium, be the suitableeest substitute of natural cordyceps bacterium.And
Through Institute of Microorganism, Academia Sinica identify, be isolatable from Zaduo County, Qinghai Province in 2011
The peacilomyce hepiahi bacterium strain SH-1 of fresh Cordyceps sinensis, is that one of which cultivation effect is preferable
Bacterial strain, range of application is wider.If but multiple bacterial strain is carried out under the same space environment simultaneously
Research or cultivation, then after artificial culture, due to the face of peacilomyce hepiahi bacterium strain SH-1
Look and the color similarity of other bacterial strains most, so its resolution is low, when it is desired to be used,
It is big that it chooses difficulty.
Summary of the invention
It is an object of the invention to provide a kind of induction peacilomyce hepiahi bacterium strain SH-1 form
The culture medium changed, this medium component is simple, it is possible to cultivate and peacilomyce hepiahi bacterium
Strain SH-1 is the readily identified peacilomyce hepiahi bacterium strain belonging to species congenerous together.
Another object of the present invention is to provide a kind of and utilize induction peacilomyce hepiahi bacterium strain
The method of the medium culture of SH-1 Morphological Transitions, the method is easy to operate, to environmental condition
Require few, it is easy to accomplish.
It is still another object of the present invention to provide a kind of utilization and induce peacilomyce hepiahi bacterium strain
The bacterial strain that the medium culture of SH-1 Morphological Transitions obtains, this bacterial strain is pink, resolution
High, it is easy to choose.
The present invention solves it and technical problem is that and realize by the following technical solutions:
A kind of culture medium inducing peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions, mainly by
Following raw material is made, and the fluid nutrient medium of every 100ml includes,
Carbon source 2～5g；
Nitrogen source 20～27g, nitrogen source includes egg；
Inorganic salts 0.05～0.31g, inorganic salts are in magnesium sulfate, dipotassium hydrogen phosphate
One or more；And
Water surplus.
A kind of medium culture utilizing induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions
Method, be included on culture medium cultivation peacilomyce hepiahi bacterium strain SH-1.
A kind of bacterial strain, utilizes above-mentioned induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions
Medium culture obtains.
Relative to prior art, the present invention includes following beneficial effect: the present invention utilizes specific
Culture medium cultivate peacilomyce hepiahi bacterium strain SH-1, this culture medium has strain growth institute
The essential elements needed, and due to the induction of this culture medium or cultivation, peacilomyce hepiahi bacterium
Strain SH-1 can occur color transition, becomes pink, and through identifying, cultivation obtains
Peacilomyce hepiahi bacterium strain SH-1 before bacterial strain and cultivation is same kind, and is used for
The effect of parasitic one-tenth Cordyceps sinensis is identical with peacilomyce hepiahi bacterium strain SH-1, so, this
Invention provides a kind of new, that can substitute peacilomyce hepiahi bacterium strain SH-1, pink
The peacilomyce hepiahi bacterium strain of look.And because most bacterial strain is all partially white, so, when
The when that peacilomyce hepiahi bacterium strain type being more, or when multiple bacterial strain coexists in same
During space, by color identifying, just can select peacilomyce hepiahi bacterium strain easily, identify
Degree height, takes conveniently, and practicality is high.
Accompanying drawing explanation
For the clearer explanation embodiment of the present invention or technical scheme of the prior art, under
The accompanying drawing used required in embodiment or description of the prior art will be briefly described by face,
It should be evident that the accompanying drawing in describing below is only some embodiments of the present invention, for
From the point of view of those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
Fig. 1 is that the different egg contents that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Produce the impact of pink bacterial strain transition probabilities；
Fig. 2 is that the different egg contents that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Impact with pink bacterial strain yield；
Fig. 3 is that the different dipotassium hydrogen phosphate content that provide of the present invention are to peacilomyce hepiahi bacterium strain
SH-1 produces the impact of pink bacterial strain transition probabilities；
Fig. 4 is that the different dipotassium hydrogen phosphate content that provide of the present invention are to peacilomyce hepiahi bacterium strain
SH-1 and the impact of pink bacterial strain yield；
Fig. 5 is that the different dry milk concentration that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Produce the impact of pink bacterial strain transition probabilities；
Fig. 6 is that the different dry milk concentration that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Impact with pink bacterial strain yield；
Fig. 7 is that the different glucose contents that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Produce the impact of pink bacterial strain transition probabilities；
Fig. 8 is that the different glucose contents that provide of the present invention are to peacilomyce hepiahi bacterium strain SH-1
Impact with pink bacterial strain yield；
Fig. 9 is the induction peacilomyce hepiahi bacterium strain SH-1 shape that the embodiment of the present invention three provides
The contour of state transition probabilities model and response surface figure；
Figure 10 is the induction peacilomyce hepiahi bacterium strain SH-1 shape that the embodiment of the present invention three provides
The contour of state transition probabilities model and response surface figure；
Figure 11 is the induction peacilomyce hepiahi bacterium strain SH-1 shape that the embodiment of the present invention three provides
The contour of state transition probabilities model and response surface figure；
Figure 12 is the induction peacilomyce hepiahi bacterium strain SH-1 shape that the embodiment of the present invention three provides
The contour of state transition probabilities model and response surface figure；
Figure 13 is that the utilization induction peacilomyce hepiahi bacterium strain SH-1 form that the present invention provides turns
The schematic diagram of the bacterial strain that the medium culture become obtains.
Detailed description of the invention
For making the purpose of the embodiment of the present invention, technical scheme and advantage clearer, below will
Technical scheme in the embodiment of the present invention is clearly and completely described.In embodiment not
Indicating actual conditions person, the condition advised according to normal condition or manufacturer is carried out.Examination used
Agent or instrument unreceipted production firm person, being can be by the commercially available conventional product bought and obtain
Product.
Induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions to the embodiment of the present invention below
Culture medium, the method for cultivation and bacterial strain be specifically described.
The culture medium of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions is mainly by following former
Material is made, and the fluid nutrient medium of every 100ml includes,
Carbon source 2～5g；
Nitrogen source 20～27g, nitrogen source includes egg；
Inorganic salts 0.05～0.31g, inorganic salts are in magnesium sulfate, dipotassium hydrogen phosphate
One or more；And
Water surplus.
Wherein, dipotassium hydrogen phosphate is free from the dipotassium hydrogen phosphate of 3 crystallizations water.It addition, need
Note, the induction peacilomyce hepiahi bacterium strain SH-1 variable color of this culture medium, described pink
It is not necessarily referring to absolute pink, as long as the color that color approximation is peach, the reddest
(such as brick-red, rose, pure red, pale red, Chinese red etc.) all be can be regarded as is this
The pink of bright middle indication.
On culture medium during static cultivation peacilomyce hepiahi bacterium strain SH-1, cultivating
Under the inducing action of base, treat that blake bottle intramatrical mycelium grows into the regular period, from the beginning of centre
Changing into pink, progressively spread to a bottle wall, original rough surface becomes tensioned, has
The smooth surface of toughness, rich in moisture, forms the most again multilayer hair bayonet fittings.Along with life
Long culture in glassware thing often forms an entirety, around combines closely with bottle wall, culture face
Look bright-coloured, overall in g., jelly-like.To peacilomyce hepiahi bacterium strain SH-1 and pink bacterial strain
ITS sequence comparison finds in identifying, 11 strain peacilomyce hepiahi bacterium strain SH-1 and two strains are pink
Bacterial strain can amplify band, and band is clear and stable, and 13 strain bacterial strains should in Genbank
Use Blast comparison, reach with the similitude of Paecilomyces sp.Cs-4 (EU328187)
100%, with three kinds of methods (ortho position phase connection, maximum likelihood method, maximum parsimony method)
13 strain bacterial strain ITS sequence and the 32 fungal strain ITS sequence being isolatable from Cordyceps sinensis (are downloaded from
Genbank) constructing system chadogram, three kinds of methods all show that 13 strain bacterium intend green grass or young crops with bat moth
Mould it is divided into one, i.e. may determine that pink bacterial strain and normal peacilomyce hepiahi bacterium strain SH-1
For same kind, i.e. Paecilomyces hepiali chen.It is to say, it is color there occurs change,
And the change of color just make peacilomyce hepiahi bacterium strain that the present invention provides belong to together bacterial strain or
Multiple bacterial strain is shown one's talent, it is easy to distinguish, fetch.The medium culture that the present invention provides
During peacilomyce hepiahi bacterium strain SH-1, its Morphological Transitions is divided into several stage, from the beginning
Without changing, to middle portions turn, again to whole transformations (seeing Figure 13) in later stage,
In transition process, mycelium etc. is gradually changing, and finally gives the bacterial strain after variable color.
The fluid nutrient medium of every 100ml also includes potato 10～12g, carrot 5～7g.
And nitrogen source also includes that milk powder, the fluid nutrient medium of every 100ml include, egg 20～25g, milk
Powder 1～2g.Vitamin in egg, milk powder is also used as the source of growth factor.It addition,
Milk powder, potato, carrot are as the part of culture medium, it is possible to for medium culture
Bacterial strain provides multiple kinds of energy material, it is also possible to simultaneously as carbon source, nitrogen source and growth factor
Source.
One or more in sucrose, glucose, fructose, peptone of carbon source, preferably
For glucose.Glucose acts not only as carbon source, it is also possible to the energy as culture medium comes
Source, and its low cost, it is easy to obtain.Certainly, carbon source can also select other, as
Molasses, grease etc..
In above-mentioned culture medium, inorganic salts are magnesium sulfate and dipotassium hydrogen phosphate (K2HPO4) mixed
Compound, the fluid nutrient medium of every 100ml includes, magnesium sulfate 0.05～0.1g, dipotassium hydrogen phosphate
0.2～0.3g.
Above-mentioned culture medium is preferably fluid nutrient medium.When selecting fluid nutrient medium to cultivate,
Between liquid medium within aqueous phase and gas phase interface (water surface is upper and lower), cover with liquid medium completely
There is Morphological Transitions in the white peacilomyce hepiahi bacterium strain SH-1 in face, and change initial
Point is that central authorities there are not the mycelia part of upstream face.
Preferably, the culture medium of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions is main
Including glucose, egg, milk powder, potato, carrot, magnesium sulfate, dipotassium hydrogen phosphate
And water.
When the Ingredient Amount of culture medium is optimized confirmation, on the basis that other composition is fixing
On, investigate K with 5 levels such as 0.1%, 0.2%, 0.3%, 0.4%, 0.5% respectively2HPO4
Consumption, observe along with K2HPO4Induction bat moth is intended green grass or young crops by proportion change in formula
The impact of trichoderma strain SH-1 Morphological Transitions, finally determines K2HPO4Addition.Become at other
On the basis of point fixing, respectively with 5 levels such as 0.5%, 1%, 1.5%, 2%, 2.5%
Investigate the consumption of milk powder, observe along with the change in formula of the milk powder proportion is to induction bat moth
The impact of Paecilomyces varioti bacterial strain SH-1 Morphological Transitions, finally determines milk powder addition.At other
On the basis of composition is fixing, respectively with 5 water such as 10%, 20%, 30%, 40%, 50%
The flat consumption investigating egg, observes along with the change in formula of the egg proportion is to induction bat
The impact of moth Paecilomyces varioti bacterial strain SH-1 Morphological Transitions, finally determines egg addition.At it
On the basis of its composition is fixing, respectively with 5 levels such as 1%, 2%, 3%, 4%, 5%
Investigate the consumption of glucose, observe along with the change in formula of the glucose proportion is to induction bat
The impact of bat Paecilomyces varioti bacterial strain SH-1 Morphological Transitions, finally determines glucose addition.
In above-mentioned experiment of single factor, when determining each composition addition, the transformation relied on is general
Rate computing formula is:
On the basis of aforementioned single factor experiment, by the response surface of 3 levels of 4 factors
Test, determines the suitableeest formulation condition of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions,
Experimental factor and level design table are shown in Table 1:
Table 1
It addition, using liquid amount 200ml as culture medium total amount, experiment fixing milk powder 5g, Portugal
Grape sugar 8g, K2HPO40.5g, select egg content be respectively 20mL, 40mL, 60mL,
80mL tests, and result can be found in Fig. 1 and Fig. 2.As shown in Figure 1, at egg content
During for 40mL, the transition probabilities that peacilomyce hepiahi bacterium strain SH-1 produces pink bacterial strain is maximum,
Fig. 2 shows, along with the increase of egg content, the yield of pink bacterial strain is also increasing, at 60mL
Time yield and 20mL, 40mL time there were significant differences, be not significantly different from 80mL.
The yield of peacilomyce hepiahi bacterium strain SH-1 increases also with the increase of egg content, and
And each gradient there were significant differences, therefore, consider pink transition probabilities and powder
The yield of red bacterial strain, selects egg content about 40mL to be advisable.
Using liquid amount 200ml as culture medium total amount, experiment fixing egg 40mL, milk powder
5g, glucose 8g, select K2HPO4Content be respectively 0.2g, 0.4g, 0.6g, 0.8g,
1g tests, and result is shown in Fig. 3 and Fig. 4.From the figure 3, it may be seen that at K2HPO4Content is
During 0.4g, the transition probabilities that peacilomyce hepiahi bacterium strain SH-1 produces pink bacterial strain is maximum,
Fig. 4 shows, along with K2HPO4The increase of content, pink bacterial strain must take the lead in increase after subtract
Little, yield difference is the most notable.The yield of peacilomyce hepiahi bacterium strain SH-1 is along with K2HPO4
The increase of content increased, and yield difference is not the most notable, therefore, selects K2HPO4Contain
Amount about 0.4g is advisable.
Using liquid amount 200ml as culture medium total amount, experiment fixing egg 40mL, K2HPO4
0.4g, glucose 8g, select dry milk concentration respectively 1g, 2g, 3g, 4g, 5g carry out
Experiment, result is shown in Fig. 5 and Fig. 6.As shown in Figure 5, when dry milk concentration is 2g, bat
The transition probabilities that moth Paecilomyces varioti bacterial strain SH-1 produces pink bacterial strain is maximum, and Fig. 6 shows, with
The increase of dry milk concentration, the yield of pink bacterial strain and peacilomyce hepiahi bacterium strain SH-1 is also
Increase therewith, and each gradient there were significant differences, therefore, select dry milk concentration 2g
It is advisable in left and right.
Using liquid amount 200ml as culture medium total amount, experiment fixing egg 40mL, K2HPO4
0.4g, milk powder 5g, select glucose content respectively 2g, 4g, 6g, 8g, 10g to carry out
Experiment, result is shown in Fig. 7 and Fig. 8.As shown in Figure 7, when glucose content is 4g, bat
The transition probabilities that bat Paecilomyces varioti bacterial strain SH-1 produces pink bacterial strain is maximum, and Fig. 8 shows,
Along with obtaining of the increase of glucose content, pink bacterial strain and peacilomyce hepiahi bacterium strain SH-1
Rate the most first increases and subtracts afterwards, and pink bacterial strain maximum occurs when glucose content is 6g, with other
Gradient is compared, significant difference, and peacilomyce hepiahi bacterium strain SH-1 at glucose content is
Maximum occurs during 8g.Therefore, glucose content about 6g is selected to be advisable.
Summary experimental result, observes the Morphological Transitions of peacilomyce hepiahi bacterium strain SH-1
The most significant culture medium prescription of probability, and adjust potato, carrot, sulphur on this basis
The proportion of acid three kinds of components of magnesium, determines the formula of this medium optimization, it is preferred, therefore, that,
The culture medium of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions is mainly by following raw material system
Becoming, the fluid nutrient medium of every 100ml includes,
It addition, utilize the culture medium training of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions
The method supported is included on culture medium cultivation peacilomyce hepiahi bacterium strain SH-1.
Further, the temperature conditions of cultivation is 18～20 DEG C.Under this temperature conditions,
Suitable peacilomyce hepiahi bacterium strain SH-1 growth.Additionally, the pH condition cultivated is preferably
5～7.
It addition, a kind of bacterial strain, utilize above-mentioned induction peacilomyce hepiahi bacterium strain SH-1 form
The medium culture changed obtains.
Below in conjunction with embodiment, inventive feature and performance are described in further detail:
Embodiment one
The cultivation of the induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions that the present embodiment provides
Base, is mainly made up of following raw material, and the fluid nutrient medium of every 100ml includes,
The present embodiment additionally provides the side of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions
Method, is included on culture medium cultivation peacilomyce hepiahi bacterium strain SH-1.
The present embodiment additionally provides according to said method, utilizes above-mentioned medium culture to obtain
Bacterial strain.
Embodiment two
The cultivation of the induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions that the present embodiment provides
Base, is mainly made up of following raw material, and the fluid nutrient medium of every 100ml includes,
The present embodiment additionally provides the side of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions
Method, is included on culture medium cultivation peacilomyce hepiahi bacterium strain SH-1, wherein, environment temperature
It it is 20 DEG C.
The present embodiment additionally provides according to said method, utilizes above-mentioned medium culture to obtain
Bacterial strain.
Embodiment three
The method present embodiments providing induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions,
It is included on culture medium cultivation peacilomyce hepiahi bacterium strain SH-1.
Wherein, condition of culture is: in 500mL glass blake bottle, liquid amount is 200mL
(culture medium), inoculum concentration is 10%, and shaking flask 10min on the shaking table of 160r/min is put
Cultivating 60 days in the cultivation that temperature is 20 DEG C, pH is 5.8.
Transition probabilities computing formula is:
First determine that the culture medium of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions includes Portugal
Grape sugar, egg, milk powder, potato, carrot, magnesium sulfate, dipotassium hydrogen phosphate and water.
Again on the basis of other composition is fixing, carry out single factor experiment.
K is investigated respectively with 0.1%, 0.2%, 0.3%, 0.4%, 0.5% these 5 levels2HPO4
Consumption, observe along with K2HPO4Proportion change in formula is to induction Paecilomyces hepiali chen
The impact of Morphological Transitions, finally determines K2HPO4Addition is 0.2%, and maximum transition probabilities is
3.75%.
The use of milk powder is investigated respectively with 0.5%, 1%, 1.5%, 2%, 2.5% these 5 levels
Amount, observes along with induction Paecilomyces hepiali chen form is turned by the change in formula of the milk powder proportion
The impact become, finally determines that milk powder addition is 1%, and maximum transition probabilities is 4.43%.
The use of egg is investigated respectively with 10%, 20%, 30%, 40%, 50% these 5 levels
Amount, observes along with induction Paecilomyces hepiali chen form is turned by the change in formula of the egg proportion
The impact become, finally determines egg addition 20%, and maximum transition probabilities is 4.58%.
The consumption of glucose is investigated respectively with 1%, 2%, 3%, 4%, 5% these 5 levels,
Observe along with the change in formula of the glucose proportion is to induction Paecilomyces hepiali chen Morphological Transitions
Impact, finally determine glucose addition 2%, maximum transition probabilities is 3.09%.
On the basis of above-mentioned single factor experiment, filter out egg (x1)、K2HPO4(x2)、
Milk powder (x3), glucose (x4) four principal elements, use Box-Behnken design knot
Close response phase method, be corresponding to induce peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions probability
Value sets up regression model, and fit equation is:
y1=-83.625+3.58750x1+31.66667x2+0.91667x3+10.58333x4+4.829
47E-015x1x2+0.1875x1x3-0.0625x1x4+25x2x3-6.25x2x4+0.625x3x4-0.0430
21x1 2-39.58333x2 2-5.33333x3 2-0.55208x4 2
The variance analysis of regression model understands, and the F=66.99 of model, P < 0.0001, this is described
Quadratic polynomial model selected by experiment has the conspicuousness of height.The F of model loses and intends
=0.291667, lose and intend item P=0.9481 > 0.05, lose and intend Xiang Jun significantly, the decision of model
Coefficients R2Value is 0.9857, illustrates that model can explain the response change of 98.57%.Cause
This, this models fitting degree is preferable, can analyze and predict induction bat moth with this model
Paecilomyces varioti Morphological Transitions probability.In total influencing factor, regression equation first order x1、x2、
Induction Paecilomyces hepiali chen Morphological Transitions probability is all had higher notable by quadratic term and mutual item
Property, the size of F value may infer that 2 factors affect y1Be ordered as: x1＞ x2。
The response surface graphics of induction Paecilomyces hepiali chen Morphological Transitions is drawn according to model of fit
With contour, can find out intuitively the peak of response surface, i.e. extreme value in parameter area with
And the impact on response that interacts between factor, may determine that optimum medium is joined successively
Side's condition and range, Design-expert 8.0 software is three-dimensional response surface and contour map after processing,
See Fig. 9～Figure 12.
To sum up, nutrient media components consumption is determined, i.e. the induction bat moth that the present embodiment provides
The culture medium of Paecilomyces varioti bacterial strain SH-1 Morphological Transitions, is mainly made up of following raw material, often
The fluid nutrient medium of 100ml includes,
The present embodiment additionally provides according to said method, utilizes above-mentioned medium culture to obtain
Bacterial strain.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention,
For a person skilled in the art, the present invention can have various modifications and variations.All
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made,
Should be included within the scope of the present invention.
Claims (10)
1. induce a culture medium for peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions, its
It is characterised by: being mainly made up of following raw material, the fluid nutrient medium of every 100ml includes,
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
The culture medium changed, it is characterised in that: the fluid nutrient medium of every 100ml also includes potato
10～12g.
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
The culture medium changed, it is characterised in that: the fluid nutrient medium of every 100ml also includes carrot
5～7g.
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
The culture medium changed, it is characterised in that: described nitrogen source also includes milk powder, the liquid of every 100ml
Body culture medium includes, described egg 20～25g, described milk powder 1～2g.
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
Change culture medium, it is characterised in that: described carbon source selected from sucrose, glucose, fructose,
One or more in peptone, preferably glucose.
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
The culture medium changed, it is characterised in that: described inorganic salts are magnesium sulfate and dipotassium hydrogen phosphate
Mixture, the fluid nutrient medium of every 100ml includes, described magnesium sulfate 0.05～0.1g, described
Dipotassium hydrogen phosphate 0.2～0.3g.
Induction peacilomyce hepiahi bacterium strain SH-1 form the most according to claim 1
The culture medium changed, it is characterised in that: mainly it is made up of following raw material, the liquid of every 100ml
Body culture medium includes,
8. the induction peacilomyce hepiahi bacterium utilized described in any one of claim 1～7
The method of the medium culture of strain SH-1 Morphological Transitions, it is characterised in that: in described cultivation
Peacilomyce hepiahi bacterium strain SH-1 is cultivated on base.
The most according to claim 8 utilization induces peacilomyce hepiahi bacterium strain SH-1
The method of Morphological Transitions medium culture, it is characterised in that: the temperature conditions of described cultivation is
18～20 DEG C.
10. a bacterial strain, it is characterised in that: utilize described in any one of claim 1～7
The medium culture of induction peacilomyce hepiahi bacterium strain SH-1 Morphological Transitions obtains.
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