CN105866090A - Ultraviolet visible light fluorescence detection system - Google Patents
Ultraviolet visible light fluorescence detection system Download PDFInfo
- Publication number
- CN105866090A CN105866090A CN201610389345.XA CN201610389345A CN105866090A CN 105866090 A CN105866090 A CN 105866090A CN 201610389345 A CN201610389345 A CN 201610389345A CN 105866090 A CN105866090 A CN 105866090A
- Authority
- CN
- China
- Prior art keywords
- light
- photodetector
- ultraviolet
- optical filter
- detecting system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001917 fluorescence detection Methods 0.000 title abstract description 5
- 230000003287 optical effect Effects 0.000 claims abstract description 68
- 238000001514 detection method Methods 0.000 claims abstract description 24
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 5
- 238000001228 spectrum Methods 0.000 claims description 47
- 229910052736 halogen Inorganic materials 0.000 claims description 7
- 150000002367 halogens Chemical class 0.000 claims description 7
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 7
- 229910052721 tungsten Inorganic materials 0.000 claims description 7
- 239000010937 tungsten Substances 0.000 claims description 7
- 238000007689 inspection Methods 0.000 claims description 6
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 claims description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 4
- 229910052805 deuterium Inorganic materials 0.000 claims description 4
- 229910052739 hydrogen Inorganic materials 0.000 claims description 4
- 239000001257 hydrogen Substances 0.000 claims description 4
- 229910000530 Gallium indium arsenide Inorganic materials 0.000 claims description 3
- 230000005693 optoelectronics Effects 0.000 claims description 3
- 230000011514 reflex Effects 0.000 claims 1
- 230000000007 visual effect Effects 0.000 abstract 2
- 238000010276 construction Methods 0.000 description 6
- 230000005284 excitation Effects 0.000 description 4
- 239000007850 fluorescent dye Substances 0.000 description 4
- 238000005286 illumination Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 206010034960 Photophobia Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000013469 light sensitivity Diseases 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000029918 bioluminescence Effects 0.000 description 1
- 238000005415 bioluminescence Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000005670 electromagnetic radiation Effects 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 230000004313 glare Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
Abstract
The invention provides an ultraviolet visible light fluorescence detection system, and relates to the technical field of detection instruments. The ultraviolet visible light fluorescence detection system comprises an exciting light device, a sample cell and a detection device which are sequentially arranged according to a light path. The exciting light device comprises a light source capable of emitting ultraviolet light and visual light and a first adjustable optical filtering mechanism used for adjusting wavelength of exciting light. The detection device comprises a second adjustable optical filtering mechanism, an optical detection mechanism and a photovoltaic conversion mechanism, the second adjustable optical filtering mechanism is used for filtering out interference light with the wavelength different from that of fluorescence, and the optical detection mechanism comprises a first optical detector used for detecting ultraviolet light, a second optical detector used for detecting visual light and a switching assembly used for switching the first optical detector and the second optical detector. The system can realize fluorescence detection within the wide wavelength range, is suitable for a PCR instrument and enlarges the detection range while ensuring accuracy of detection results of solution concentration.
Description
Technical field
The present invention relates to detecting instrument technical field, be specifically related to a kind of ultraviolet-visible fluoroscopic examination system
System.
Background technology
Along with the development of bioluminescence imaging technique, increasing fluorescence detector is used for food peace
Entirely, the field such as industrial detection, medicament research and development and medical diagnosis on disease.It addition, at the PCR of biomedical sector
(polymerase chain reaction) instrument can also utilize fluoroscopic examination realization automatic, quick to target gene concentration
Detection.
Existing fluorescence detector usually utilizes ultraviolet light as excitation light irradiation sample, then utilizes
The visible fluorescence of photodetector reception electromagnetic radiation, and detect the intensity of this fluorescence, then utilize fluorescence
Quantification of intensities calculates the fluorescent material concentration in solution.Along with the progress of technology, in order to expand fluorescence inspection
Survey range, in prior art also have use visible ray as exciting light or use wavelength more
Short ultraviolet light is probably visible ray is also likely to be ultraviolet light as exciting light, the fluorescence therefore launched.
But, the light source that existing fluorescence detector generally uses is more single, the wave-length coverage of optical receiver
The most narrow, therefore, if it is desired to realize simultaneously ultraviolet-visible fluoroscopic examination the most at least need two glimmering
Optical detector realizes light source and the fluoroscopic examination of different wave length.
Summary of the invention
Therefore, present invention aim to overcome that to need to use multiple stage instrument present in prior art real
The defect of the quantitative fluorescence detection of existing Uv and visible light wave band.
To this end, the present invention provides following technical scheme:
A kind of ultraviolet-visible fluorescence detecting system, including the excitation-light unit set gradually according to light path,
Sample cell and detection device, excitation-light unit include sending ultraviolet light and the light source of visible ray, the
One adjustable optical filter mechanism, the first adjustable optical filter mechanism is for adjusting the wavelength of exciting light；Inspection
Surveying device and include the second adjustable optical filter mechanism, light detecting mechanism and opto-electronic conversion mechanism, second can
Light modulation learns filter mechanism for filtering the interference light that wavelength is inconsistent with wavelength of fluorescence, light detecting mechanism bag
Include the first photodetector for detecting ultraviolet light, for detecting the second photodetector and the use of visible ray
In switching the first photodetector and the changeover module of the second photodetector.
Preferably, the first adjustable optical filter mechanism includes the first spectrum groupware and/or the second light splitting group
Part, the first spectrum groupware includes the first entrance slit, the first incident collimation set gradually according to light path
Mirror, the first prism, the first outgoing collimating mirror and the first exit slit；Second spectrum groupware include according to
The second entrance slit that light path sets gradually, the second incident collimating mirror, the second grating, the second outgoing are accurate
Straight mirror and the second exit slit.
Preferably, the second adjustable optical filter mechanism includes the 3rd spectrum groupware and/or the 4th light splitting group
Part, the 3rd spectrum groupware includes the 3rd entrance slit, the 3rd incident collimation set gradually according to light path
Mirror, prism, the 3rd outgoing collimating mirror and the 3rd exit slit；4th spectrum groupware include according to
The 4th entrance slit that light path sets gradually, the 4th incident collimating mirror, the 4th grating, the 4th outgoing are accurate
Straight mirror and the 4th exit slit.
Preferably, the first adjustable optical filter mechanism includes the first filter wheel, the first drive mechanism
With the first motor, the first filter wheel is provided with the optical filter of multiple different wave length, and the first motor leads to
Crossing the first drive mechanism drives the first filter wheel to rotate.
Preferably, the second adjustable optical filter mechanism includes the second filter wheel, the second drive mechanism
With the second motor, the second filter wheel is provided with the optical filter of multiple different wave length, and the second motor leads to
Crossing the second drive mechanism drives the second filter wheel to rotate.
Preferably, light source includes halogen tungsten lamp and hydrogen lamp and switching circuit, or includes halogen tungsten lamp and deuterium lamp
And switching circuit, or include that multiple light emitting diode and switching circuit, multiple diodes send
Optical wavelength is different.
Preferably, the first photodetector is photo-multiplier pipe detector, and the second photodetector is InGaAs
Detector.
Preferably, changeover module includes illuminator, for by the fluorescent reflection of directive the first photodetector
To the second photodetector, or it is used for the fluorescent reflection of directive the second photodetector to the first photodetector.
Preferably, changeover module includes photodetector rotating disk, the first photodetector and the second photodetector
It is arranged on photodetector rotating disk.
Preferably, changeover module includes integrating sphere, the first photodetector and the incidence of the second photodetector
Face is towards the internal face of integrating sphere and the most not relative with the entrance port of fluorescence.
Technical solution of the present invention, has the advantage that
The ultraviolet-visible fluorescence detecting system that technical solution of the present invention provides, by an instrument
Arrange and can send the light source of ultraviolet light and visible ray and for detecting the first light detection of ultraviolet light
Device, for detecting the second photodetector of visible ray and changeover module achieves the fluorescence of wide wave-length coverage
Detection, and select different light sources for different fluorescence excitation demands, for different fluorescence ripples
The photodetector that long selection is different, can be greatly improved the precision of fluoroscopic examination.When this system is for PCR
Instrument or for other solution concentrations of detection by quantitative time, it is ensured that the degree of accuracy of Concentration Testing result, with
The multiple fluorescent dye of Shi Peihe can be greatly promoted the scope of application of fluoroscopic examination.It addition, also set up
The second adjustable optical filter mechanism is for filtering the exciting light transmitted through sample cell and other interference
Light, thus improve the precision of this fluorescence detecting system further.
Accompanying drawing explanation
In order to be illustrated more clearly that the specific embodiment of the invention or technical scheme of the prior art, under
The accompanying drawing used required in detailed description of the invention or description of the prior art will be briefly described by face,
It should be evident that the accompanying drawing in describing below is some embodiments of the present invention, general for this area
From the point of view of logical technical staff, on the premise of not paying creative work, it is also possible to obtain according to these accompanying drawings
Obtain other accompanying drawing.
Fig. 1 is the structured flowchart of the embodiment of the present invention 1 medium ultraviolet visible fluorescence detecting system；
Fig. 2 is the structural representation of the first spectrum groupware in the embodiment of the present invention 1；
Fig. 3 is the structural representation of the second spectrum groupware in the embodiment of the present invention 1；
Fig. 4 is changeover module switching principle schematic diagram in the embodiment of the present invention 1.
Reference: 1-excitation-light unit, 11-light source, 12-the first adjustable optical filter mechanism, 121-
First spectrum groupware, 1211-the first entrance slit, the incident collimating mirror of 1212-first, 1213-the first rib
Mirror, 1214-the first outgoing collimating mirror, 1215-the first exit slit, 122-the second spectrum groupware, 1221-
Second entrance slit, the incident collimating mirror of 1222-second, 1223-the second grating, 1224-the second outgoing is accurate
Straight mirror, 1225-the second exit slit, 2-sample cell, 3-detects device, 31-the second adjustable optical mistake
Filter structure, 32-light detecting mechanism, 321-the first photodetector, 322-changeover module, 323-the second light
Detector, 33-opto-electronic conversion mechanism.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme is clearly and completely described.Obviously,
Described embodiment is a part of embodiment of the present invention rather than whole embodiments.Based on this
Embodiment in bright, those of ordinary skill in the art are obtained under not making creative work premise
Every other embodiment, broadly fall into the scope of protection of the invention.
In describing the invention, it should be noted that term " " center ", " on ", D score, " left ",
Orientation or the position relationship of the instruction such as " right ", " vertically ", " level ", " interior ", " outward " are based on attached
Orientation shown in figure or position relationship, be for only for ease of description the present invention and simplify describe rather than
Instruction or the hint device of indication or element must have specific orientation, with specific azimuth configuration and
Operation, is therefore not considered as limiting the invention.Additionally, term " first ", " second ", "
Three " it is only used for describing purpose, and it is not intended that indicate or hint relative importance.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, art
Language " is installed ", " being connected ", " connection " should be interpreted broadly, and connects for example, it may be fixing,
Can also be to removably connect, or be integrally connected；Can be to be mechanically connected, it is also possible to be electrical connection；
Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, it is also possible to be two element internals
Connection, can be wireless connections, it is also possible to be wired connection.Ordinary skill people for this area
For Yuan, above-mentioned term concrete meaning in the present invention can be understood with concrete condition.
As long as additionally, technical characteristic involved in invention described below difference embodiment that
The conflict of not constituting between this just can be combined with each other.
Embodiment 1
As it is shown in figure 1, the present embodiment provides a kind of ultraviolet-visible fluorescence detecting system, can be suitable for
In PCR instrument, including the excitation-light unit 1 set gradually according to light path, sample cell 2 and detection device 3,
Excitation-light unit 1 includes that light source the 11, first adjustable optical that can send ultraviolet light and visible ray filters
Mechanism 12, the first adjustable optical filter mechanism 12 is for adjusting the wavelength of exciting light, and detection device 3 wraps
Including the second adjustable optical filter mechanism 31, light detecting mechanism 32 and opto-electronic conversion mechanism 33, second can
Light modulation learns filter mechanism 31 for filtering the interference light that wavelength is inconsistent with wavelength of fluorescence, light detecting mechanism
32 include the first photodetector 321 for detecting ultraviolet light, for detecting the second light inspection of visible ray
Survey device 323 and for switching the first photodetector 321 and changeover module 322 of the second photodetector 323.
Specifically, light source 11 includes halogen tungsten lamp and hydrogen lamp and switching circuit, or includes halogen tungsten lamp and deuterium
Lamp and switching circuit, or include multiple light emitting diode and switching circuit, plurality of diode
The optical wavelength sent is different.Halogen tungsten lamp is used for providing the exciting light of visible light wave range, deuterium lamp and hydrogen lamp
In the exciting light providing ultraviolet band.Different light emitting diodes can send the light that wavelength is different, because of
This present embodiment can use various different light emitting diode combined as wide wavelength light source.
The ultraviolet-visible fluorescence detecting system that the present embodiment provides, can by arranging on an instrument
With send ultraviolet light and visible ray light source 11 and for detect ultraviolet light the first photodetector 321,
The second photodetector 323 and changeover module 322 for detecting visible ray achieves wide wave-length coverage
Fluoroscopic examination, and select different light sources for different fluorescence excitation demand, for different glimmering
Optical wavelength selects different photodetectors can be greatly improved the precision of fluoroscopic examination.When this system is used for
PCR instrument or for other solution concentrations of detection by quantitative time, it is ensured that the degree of accuracy of Concentration Testing result.
It addition, be also provided with the second adjustable optical filter mechanism 31 for filtering exciting transmitted through sample cell 2
Light and other interference light, thus improve the precision of this fluorescence detecting system further.
As specific embodiment, the first adjustable optical filter mechanism 12 includes the first spectrum groupware 121
Or second spectrum groupware 122, as shown in Figure 2 (only schematic diagram, not as each component shape and relatively
The restriction of position relationship), the first spectrum groupware 121 includes that the first incidence set gradually according to light path is narrow
Incident collimating mirror the 1212, first prism the 1213, first outgoing collimating mirror 1214 and of seam 1211, first
First exit slit 1215.As shown in Figure 3 (only schematic diagram, not as each component shape and relatively
The restriction of position relationship), the second spectrum groupware 122 includes that the second incidence set gradually according to light path is narrow
Incident collimating mirror the 1222, second grating the 1223, second outgoing collimating mirror 1224 and of seam 1221, second
Second exit slit 1225.In other detailed description of the invention, the first adjustable optical filter mechanism 12
The first spectrum groupware 121 and the second spectrum groupware 122 of series connection can be included.In the present embodiment, although
Can by switching circuit select the different luminous bodys in light source 11 to meet fluorescence excitation demand, but not
The optical wavelength sent with luminous body is the most relatively wide, and a kind of fluorescent dye the most only absorbs a kind of wavelength
Exciting light, so the present embodiment is provided with the first adjustable optical filter mechanism 12 to select fluorescence staining
The light of agent corresponding wavelength, as exciting light, filters the light of other wavelength.
Above-mentioned first spectrum groupware 121 is prismatic decomposition assembly, and the first entrance slit 1211 is a fixed width
The strip light hole of degree, as the incident illumination passage of this spectrum groupware.First entrance slit 1211
Width determines the band of this spectrum groupware together with the first prism 1213 and other light channel structures
Wide.First incident collimating mirror 1212 is become for the incident illumination will injected by the first entrance slit 1211
It is irradiated to the first prism 1213 for directional light.This first prism 1213 is beam splitter, utilizes its dispersion
Mechanism carries out light splitting.Parallel for by through the first prism 1213 light splitting of first outgoing collimating mirror 1214
Light converges at the first exit slit 1215.
Above-mentioned second spectrum groupware 122 is grating beam splitting assembly, and the second entrance slit 1221 is also certain
The strip light hole of width, as the incident illumination passage of the second spectrum groupware 122.Second is incident accurate
Straight mirror 1222 is irradiated to for the incident illumination injected by the second entrance slit 1221 is become directional light
Second grating 1223, this second grating 1223 is beam splitter, utilizes its diffraction interference principle to carry out point
Light, the second outgoing collimating mirror 1224 for converging at the by the directional light through the second grating 1223 light splitting
Two exit slits 1225.
The first spectrum groupware 121 and the second spectrum groupware 122 for connecting are as the first tunable optical
Learn in the embodiment of filter mechanism 12, through twice light splitting, excitation-light unit 1 can be substantially increased and send
Exciting light purity, exciting light i.e. seldom has the veiling glare beyond wavelength light needed for fluorescent dye.
It addition, the second adjustable optical filter mechanism 31 includes the 3rd spectrum groupware or the 4th spectrum groupware,
The 3rd entrance slit that 3rd spectrum groupware includes setting gradually according to light path, the 3rd incident collimating mirror,
Prism, the 3rd outgoing collimating mirror and the 3rd exit slit；4th spectrum groupware includes according to light path
The 4th entrance slit that sets gradually, the 4th incident collimating mirror, the 4th grating, the 4th outgoing collimating mirror
With the 4th exit slit.In other detailed description of the invention, the second adjustable optical filter mechanism 31 can
The 3rd spectrum groupware and the 4th spectrum groupware including series connection.
The filtering principles of the 3rd spectrum groupware and the light-dividing principle of the first spectrum groupware 121 are consistent, and the 4th
The filtering principles of spectrum groupware and the light-dividing principle of the second spectrum groupware 122 are consistent.3rd spectrum groupware
Structure is similar with the first spectrum groupware 121, the structure of the 4th spectrum groupware and the second spectrum groupware 122
Similar.Owing to the 3rd spectrum groupware and the 4th spectrum groupware are mainly used in filtering the interference launched in fluorescence
Light, and need the light intensity according to fluorescence to carry out detection by quantitative sample concentration, therefore its parameters according to
It is actually needed adjustment.
As optional embodiment, the first photodetector 321 is photo-multiplier pipe detector, the
Two photodetectors 323 are InGaAs detector.In other detailed description of the invention, the first light detection
Device 321 can also be other photodetectors, or includes multiple photodetector, each photodetector pair
Different-waveband sensitivity in ultraviolet light is different.Similarly, the second photodetector 323 can also be
Other visible light detectors, or include multiple visible light detector, each visible light detector is to can
See that the different-waveband lightsensitivity in light is different.
Further, and as shown in Figure 4 (only principle schematic, not as each component shape and relatively
The restriction of position relationship), changeover module 322 includes illuminator, for by directive the first photodetector 321
Fluorescent reflection to the second photodetector 323, or for by anti-for the fluorescence of directive the second photodetector 323
It is incident upon the first photodetector 321.
Although the detection wavelength of photodetector has certain width, but photodetector is to different wave length
Lightsensitivity different.Only utilize the high photodetector of certain wavelength sensitivity to detect correspondence
The light of wavelength, the accuracy of guarantee light-intensity test.Therefore, the present embodiment is provided with the inspection of multiple light
Survey device, then select suitable photodetector according to the light intensity launching fluorescence, thus ensure that light is examined
The accuracy surveyed.Illuminator is used to switch photodetector, simple in construction, it is easy to accomplish, convenient behaviour
Make.
Embodiment 2
Originally executing example and provide a kind of ultraviolet-visible fluorescence detecting system, the difference with above-described embodiment 1 exists
In:
First adjustable optical filter mechanism 12 includes the first filter wheel, the first drive mechanism and first
Motor, the first filter wheel is provided with the optical filter of multiple different wave length, and the first motor passes through first
Drive mechanism drives the first filter wheel to rotate.
The present embodiment selects the light with fluorescent dye corresponding wavelength as exciting light by optical filter,
Filter the light of other wavelength.And optical filter is arranged on the first filter wheel, by rotating rotating disk
Just can realize the switching of optical filter, simple in construction, easy to operate.
Embodiment 3
Originally executing example and provide a kind of ultraviolet-visible fluorescence detecting system, the difference with above-described embodiment 1 exists
In:
Second adjustable optical filter mechanism 31 includes the second filter wheel, the second drive mechanism and second
Motor, the second filter wheel is provided with the optical filter of multiple different wave length, and the second motor passes through second
Drive mechanism drives the second filter wheel to rotate.
The present embodiment filters the interference light launched in fluorescence by optical filter.And optical filter is arranged on
On second filter wheel, just can realize the switching of optical filter by rotating rotating disk, simple in construction,
Easy to operate.
Embodiment 4
Originally executing example and provide a kind of ultraviolet-visible fluorescence detecting system, the difference with above-described embodiment 1 exists
In:
First adjustable optical filter mechanism 12 includes the first filter wheel, the first drive mechanism and first
Motor, the first filter wheel is provided with the optical filter of multiple different wave length, and the first motor passes through first
Drive mechanism drives the first filter wheel to rotate.The present embodiment is selected by optical filter and is contaminated with fluorescence
The light of toner corresponding wavelength, as exciting light, filters the light of other wavelength.And optical filter is arranged on
On one filter wheel, just can realize the switching of optical filter, simple in construction, behaviour by rotating rotating disk
Facilitate.
Second adjustable optical filter mechanism 31 includes the second filter wheel, the second drive mechanism and second
Motor, the second filter wheel is provided with the optical filter of multiple different wave length, and the second motor passes through second
Drive mechanism drives the second filter wheel to rotate.The present embodiment filters transmitting fluorescence by optical filter
In interference light.And optical filter is arranged on the second filter wheel, the most permissible by rotating rotating disk
Realize the switching of optical filter, simple in construction, easy to operate.
Embodiment 5
Originally execute example and provide a kind of ultraviolet-visible fluorescence detecting system, with the difference of above-described embodiment 1-4
It is:
Changeover module 322 includes photodetector rotating disk, the first photodetector 321 and the second photodetector
323 are arranged on photodetector rotating disk.
The present embodiment switches photodetector by arranging photodetector rotating disk, and simple in construction is easy to operate.
This photodetector rotating disk can hand rotation, it is also possible to rotated by driven by motor.
Embodiment 6
Originally execute example and provide a kind of ultraviolet-visible fluorescence detecting system, with the difference of above-described embodiment 1-4
It is:
Changeover module 322 includes integrating sphere, the first photodetector 321 and the second photodetector 323
The plane of incidence is towards the internal face of integrating sphere and the most not relative with the entrance port of fluorescence.
The present embodiment realizes the different photodetector detection to different wavelength fluorescent by integrating sphere,
Owing to need not movable parts to change light path or switching photodetector, therefore its reliability is high.
Furthermore it is also possible to use multiple photodetector to carry out the detection of fluorescence intensity simultaneously, then select inspection
Survey the most reliable fluorescence intensity of result and complete interpretation of result.
Obviously, above-described embodiment is only for clearly demonstrating example, and not to embodiment party
The restriction of formula.For those of ordinary skill in the field, the most also may be used
To make other changes in different forms.Here without also all of embodiment being given
With exhaustive.And the obvious change thus extended out or variation are still in the guarantor of the invention
Protect among scope.
Claims (10)
1. a ultraviolet-visible fluorescence detecting system, including the exciting light dress set gradually according to light path
Put, sample cell and detection device, it is characterised in that described excitation-light unit includes sending ultraviolet
Light and the light source of visible ray, the first adjustable optical filter mechanism, described first adjustable optical filter mechanism
For adjusting the wavelength of exciting light；Described detection device includes that the second adjustable optical filter mechanism, light are examined
Surveying mechanism and opto-electronic conversion mechanism, described second adjustable optical filter mechanism is used for filtering wavelength and fluorescence
The interference light that wavelength is inconsistent, described light detecting mechanism includes the first light detection for detecting ultraviolet light
Device, for detecting the second photodetector of visible ray and for switching described first photodetector and described
The changeover module of the second photodetector.
Ultraviolet-visible fluorescence detecting system the most according to claim 1, it is characterised in that institute
State the first adjustable optical filter mechanism and include the first spectrum groupware and/or the second spectrum groupware, described first
The first entrance slit that spectrum groupware includes setting gradually according to light path, the first incident collimating mirror, first
Prism, the first outgoing collimating mirror and the first exit slit；Described second spectrum groupware includes according to light path
The second entrance slit of setting gradually, the second incident collimating mirror, the second grating, the second outgoing collimating mirror
With the second exit slit.
Ultraviolet-visible fluorescence detecting system the most according to claim 1 and 2, it is characterised in that
Described second adjustable optical filter mechanism includes the 3rd spectrum groupware and/or the 4th spectrum groupware, described
The 3rd entrance slit that three spectrum groupware include setting gradually according to light path, the 3rd incident collimating mirror, the
Prism, the 3rd outgoing collimating mirror and the 3rd exit slit；Described 4th spectrum groupware includes according to light
The 4th entrance slit that road sets gradually, the 4th incident collimating mirror, the 4th grating, the 4th outgoing collimation
Mirror and the 4th exit slit.
4., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-3, it is special
Levying and be, described first adjustable optical filter mechanism includes the first filter wheel, the first drive mechanism
With the first motor, described first filter wheel is provided with the optical filter of multiple different wave length, and described
One motor drives described first filter wheel to rotate by described first drive mechanism.
5., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-4, it is special
Levying and be, described second adjustable optical filter mechanism includes the second filter wheel, the second drive mechanism
With the second motor, described second filter wheel is provided with the optical filter of multiple different wave length, and described
Two motors drive described second filter wheel to rotate by described second drive mechanism.
6., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-5, it is special
Levying and be, described light source includes halogen tungsten lamp and hydrogen lamp and switching circuit, or includes halogen tungsten lamp and deuterium lamp
And switching circuit, or include that multiple light emitting diode and switching circuit, the plurality of diode are sent out
The optical wavelength gone out is different.
7., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-6, it is special
Levying and be, described first photodetector is photo-multiplier pipe detector, and described second photodetector is
InGaAs detector.
8., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-7, it is special
Levying and be, described changeover module includes illuminator, for by the fluorescence of the first photodetector described in directive
Reflex to described second photodetector, or for by the fluorescent reflection of the second photodetector described in directive extremely
Described first photodetector.
9., according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-7, it is special
Levying and be, described changeover module includes photodetector rotating disk, described first photodetector and described second
Photodetector is arranged on described photodetector rotating disk.
10. according to the ultraviolet-visible fluorescence detecting system according to any one of claim 1-7, its
Being characterised by, described changeover module includes integrating sphere, described first photodetector and described second light inspection
Survey the plane of incidence of device towards the internal face of described integrating sphere and the most not relative with the entrance port of fluorescence.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610389345.XA CN105866090A (en) | 2016-06-03 | 2016-06-03 | Ultraviolet visible light fluorescence detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610389345.XA CN105866090A (en) | 2016-06-03 | 2016-06-03 | Ultraviolet visible light fluorescence detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105866090A true CN105866090A (en) | 2016-08-17 |
Family
ID=56675916
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610389345.XA Pending CN105866090A (en) | 2016-06-03 | 2016-06-03 | Ultraviolet visible light fluorescence detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105866090A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770086A (en) * | 2016-11-24 | 2017-05-31 | 北京旌准医疗科技有限公司 | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument |
| CN107621457A (en) * | 2017-09-27 | 2018-01-23 | 中国科学院理化技术研究所 | A kind of DUV frequency multiplication test device |
| CN109211789A (en) * | 2018-10-31 | 2019-01-15 | 大连依利特分析仪器有限公司 | Fluorescence detection ultraviolet LED automatic switching control equipment |
| CN110018123A (en) * | 2019-05-16 | 2019-07-16 | 厦门斯坦道科学仪器股份有限公司 | A kind of In situ spectroscopic fado parameter water quality monitoring method and device |
| CN110161228A (en) * | 2019-06-19 | 2019-08-23 | 上海菲伽生物科技有限公司 | Continuous ultraviolet excitation fluorescence spectrum colour filter method immunologic detection method |
| CN110346317A (en) * | 2019-06-19 | 2019-10-18 | 浙江欧文光谱科技有限公司 | UV, visible light Fluorescence Spectrometer |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1768151A (en) * | 2003-04-04 | 2006-05-03 | 霍夫曼-拉罗奇有限公司 | Improved system for multicolor real time PCR |
| CN1869614A (en) * | 2005-05-25 | 2006-11-29 | 株式会社岛津制作所 | Detection device of UV visual near-infrared sepctrophotometer |
| JP2007193386A (en) * | 2006-01-17 | 2007-08-02 | Dainippon Printing Co Ltd | Security information visual determination device |
| CN201075088Y (en) * | 2007-09-20 | 2008-06-18 | 北京路捷仪器有限公司 | Multicenter atomic fluorescence optical spectrometer |
| CN101699271A (en) * | 2009-10-27 | 2010-04-28 | 西安天隆科技有限公司 | Real-time fluorescence quantitative PCR excitation detection system |
| CN102279174A (en) * | 2011-07-15 | 2011-12-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | Alga identification and measurement sensor and method |
| CN102410879A (en) * | 2010-08-24 | 2012-04-11 | 索尼公司 | Fluorescence intensity correction method, fluorescence intensity calculation method, and fluorescence intensity calculation device and fluorescence intensity correction program |
| CN202994746U (en) * | 2012-12-11 | 2013-06-12 | 深圳市虹彩检测技术有限公司 | Switching device for plurality of types of detectors of liquid chromatograph and detection system of liquid chromatograph |
| CN203688069U (en) * | 2013-11-26 | 2014-07-02 | 武进田 | Ultraviolet, visible, near-infrared spectrophotometer echelette grating light-splitting device |
| CN104865331A (en) * | 2015-03-09 | 2015-08-26 | 俞嘉德 | High-performance liquid chromatography dual-purpose ultraviolet visible light and fluorescence dual-spectrum dual-detector |
| CN105606579A (en) * | 2016-02-18 | 2016-05-25 | 苏州晋翌生物医学仪器有限公司 | Fluorescence detection system and multi-channel detection device |
| CN205280679U (en) * | 2015-12-26 | 2016-06-01 | 东莞市精准通检测服务有限公司 | Auto -change over device of multiple detector of liquid chromatograph |
-
2016
- 2016-06-03 CN CN201610389345.XA patent/CN105866090A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1768151A (en) * | 2003-04-04 | 2006-05-03 | 霍夫曼-拉罗奇有限公司 | Improved system for multicolor real time PCR |
| CN1869614A (en) * | 2005-05-25 | 2006-11-29 | 株式会社岛津制作所 | Detection device of UV visual near-infrared sepctrophotometer |
| JP2007193386A (en) * | 2006-01-17 | 2007-08-02 | Dainippon Printing Co Ltd | Security information visual determination device |
| CN201075088Y (en) * | 2007-09-20 | 2008-06-18 | 北京路捷仪器有限公司 | Multicenter atomic fluorescence optical spectrometer |
| CN101699271A (en) * | 2009-10-27 | 2010-04-28 | 西安天隆科技有限公司 | Real-time fluorescence quantitative PCR excitation detection system |
| CN102410879A (en) * | 2010-08-24 | 2012-04-11 | 索尼公司 | Fluorescence intensity correction method, fluorescence intensity calculation method, and fluorescence intensity calculation device and fluorescence intensity correction program |
| CN102279174A (en) * | 2011-07-15 | 2011-12-14 | 中国科学院苏州纳米技术与纳米仿生研究所 | Alga identification and measurement sensor and method |
| CN202994746U (en) * | 2012-12-11 | 2013-06-12 | 深圳市虹彩检测技术有限公司 | Switching device for plurality of types of detectors of liquid chromatograph and detection system of liquid chromatograph |
| CN203688069U (en) * | 2013-11-26 | 2014-07-02 | 武进田 | Ultraviolet, visible, near-infrared spectrophotometer echelette grating light-splitting device |
| CN104865331A (en) * | 2015-03-09 | 2015-08-26 | 俞嘉德 | High-performance liquid chromatography dual-purpose ultraviolet visible light and fluorescence dual-spectrum dual-detector |
| CN205280679U (en) * | 2015-12-26 | 2016-06-01 | 东莞市精准通检测服务有限公司 | Auto -change over device of multiple detector of liquid chromatograph |
| CN105606579A (en) * | 2016-02-18 | 2016-05-25 | 苏州晋翌生物医学仪器有限公司 | Fluorescence detection system and multi-channel detection device |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106770086A (en) * | 2016-11-24 | 2017-05-31 | 北京旌准医疗科技有限公司 | The fluorescence detection method and system of a kind of real-time fluorescence quantitative PCR instrument |
| CN107621457A (en) * | 2017-09-27 | 2018-01-23 | 中国科学院理化技术研究所 | A kind of DUV frequency multiplication test device |
| CN109211789A (en) * | 2018-10-31 | 2019-01-15 | 大连依利特分析仪器有限公司 | Fluorescence detection ultraviolet LED automatic switching control equipment |
| CN110018123A (en) * | 2019-05-16 | 2019-07-16 | 厦门斯坦道科学仪器股份有限公司 | A kind of In situ spectroscopic fado parameter water quality monitoring method and device |
| CN110161228A (en) * | 2019-06-19 | 2019-08-23 | 上海菲伽生物科技有限公司 | Continuous ultraviolet excitation fluorescence spectrum colour filter method immunologic detection method |
| CN110346317A (en) * | 2019-06-19 | 2019-10-18 | 浙江欧文光谱科技有限公司 | UV, visible light Fluorescence Spectrometer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105866090A (en) | Ultraviolet visible light fluorescence detection system | |
| CN105973855A (en) | Infrared visible light fluorescence detection system | |
| ES2359968T3 (en) | SPECTROPHOTOMETRIC AND NEFELOMETRIC DETECTION UNIT. | |
| JP5235681B2 (en) | Modular fluorescence and photometric reader | |
| CN101706434B (en) | Fluorophototmeter capable of detecting light absorption or light transmission | |
| JP2015518157A (en) | Method and apparatus for measuring absorbance of substances in solution | |
| JP6501714B2 (en) | Optical survey device | |
| JP5824325B2 (en) | System for scattering and absorption analysis | |
| CN105223137B (en) | A kind of optical measuring device for detection of biological samples | |
| CN104198385B (en) | Eight-channel device for detecting switchable light source absorption spectrum | |
| JP6804445B2 (en) | Integration of fluorescence detection function into absorbance measuring device | |
| EP3198268B1 (en) | An improved apparatus for measuring hexavalent chromium in water | |
| CN103018214B (en) | A kind of reflection type optical path transient state absorption spectrometer | |
| CN104597011A (en) | Excitation light source drift correction device and fluorescence spectrograph | |
| JPS62192632A (en) | Microscope photometer | |
| ES2690570T3 (en) | Procedure and device for the determination of the fluorescence of a sample as well as its use | |
| CN106092968A (en) | Optical detection apparatus and method | |
| KR20150016384A (en) | Shutter assembly for a luminescence-based sample analyzer | |
| CA3099111C (en) | Illumination unit with multiple light sources for generating a uniform illumination spot | |
| JP2015114298A (en) | Spectrophotometer and analysis system | |
| CN109477795B (en) | Method and system for optical-based measurements with selectable excitation light path | |
| RU2463568C1 (en) | Apparatus for thermal lens spectroscopy | |
| CN211061419U (en) | Optical system and detector | |
| CN111413327A (en) | Dual mode detection system and dual mode detection method | |
| CN211122535U (en) | Fluorescence detector |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160817 |
|
| RJ01 | Rejection of invention patent application after publication |