CN105865888B - 细胞蜡块的制备系统 - Google Patents
细胞蜡块的制备系统 Download PDFInfo
- Publication number
- CN105865888B CN105865888B CN201610187761.1A CN201610187761A CN105865888B CN 105865888 B CN105865888 B CN 105865888B CN 201610187761 A CN201610187761 A CN 201610187761A CN 105865888 B CN105865888 B CN 105865888B
- Authority
- CN
- China
- Prior art keywords
- tube body
- conduit
- test tube
- tube
- fixing piece
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000012360 testing method Methods 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 239000012528 membrane Substances 0.000 claims abstract description 34
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 30
- 230000001413 cellular effect Effects 0.000 claims abstract description 9
- 239000002184 metal Substances 0.000 claims description 12
- 238000011897 real-time detection Methods 0.000 claims description 2
- 210000004027 cell Anatomy 0.000 description 44
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 22
- 238000000034 method Methods 0.000 description 19
- 239000012188 paraffin wax Substances 0.000 description 19
- 230000008569 process Effects 0.000 description 12
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 10
- 230000018044 dehydration Effects 0.000 description 9
- 238000006297 dehydration reaction Methods 0.000 description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 230000006378 damage Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000001124 body fluid Anatomy 0.000 description 3
- 239000010839 body fluid Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000007170 pathology Effects 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000008520 organization Effects 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 1
- 206010048612 Hydrothorax Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000005357 flat glass Substances 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052738 indium Inorganic materials 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2873—Cutting or cleaving
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610187761.1A CN105865888B (zh) | 2014-04-23 | 2014-04-23 | 细胞蜡块的制备系统 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610187761.1A CN105865888B (zh) | 2014-04-23 | 2014-04-23 | 细胞蜡块的制备系统 |
CN201410166957.3A CN103969096B (zh) | 2014-04-23 | 2014-04-23 | 自动制备细胞蜡块的装置及其方法 |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410166957.3A Division CN103969096B (zh) | 2014-04-23 | 2014-04-23 | 自动制备细胞蜡块的装置及其方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105865888A CN105865888A (zh) | 2016-08-17 |
CN105865888B true CN105865888B (zh) | 2019-02-22 |
Family
ID=51238864
Family Applications (8)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610254620.7A Active CN105928757B (zh) | 2014-04-23 | 2014-04-23 | 自动式细胞蜡块制备系统 |
CN201610204152.2A Expired - Fee Related CN105890944B (zh) | 2014-04-23 | 2014-04-23 | 具有机械臂的细胞蜡块制备装置 |
CN201610172989.3A Expired - Fee Related CN105628477B (zh) | 2014-04-23 | 2014-04-23 | 全自动快速制备细胞蜡块的装置 |
CN201610182572.5A Active CN105865864B (zh) | 2014-04-23 | 2014-04-23 | 具有快速制备功能的细胞蜡块加工装置 |
CN201610186890.9A Active CN105842028B (zh) | 2014-04-23 | 2014-04-23 | 具有加压、抽取功能的细胞蜡块制备装置 |
CN201610187761.1A Active CN105865888B (zh) | 2014-04-23 | 2014-04-23 | 细胞蜡块的制备系统 |
CN201410166957.3A Active CN103969096B (zh) | 2014-04-23 | 2014-04-23 | 自动制备细胞蜡块的装置及其方法 |
CN201610191848.6A Active CN105890943B (zh) | 2014-04-23 | 2014-04-23 | 具有自动检测功能的细胞蜡块制备装置 |
Family Applications Before (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610254620.7A Active CN105928757B (zh) | 2014-04-23 | 2014-04-23 | 自动式细胞蜡块制备系统 |
CN201610204152.2A Expired - Fee Related CN105890944B (zh) | 2014-04-23 | 2014-04-23 | 具有机械臂的细胞蜡块制备装置 |
CN201610172989.3A Expired - Fee Related CN105628477B (zh) | 2014-04-23 | 2014-04-23 | 全自动快速制备细胞蜡块的装置 |
CN201610182572.5A Active CN105865864B (zh) | 2014-04-23 | 2014-04-23 | 具有快速制备功能的细胞蜡块加工装置 |
CN201610186890.9A Active CN105842028B (zh) | 2014-04-23 | 2014-04-23 | 具有加压、抽取功能的细胞蜡块制备装置 |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410166957.3A Active CN103969096B (zh) | 2014-04-23 | 2014-04-23 | 自动制备细胞蜡块的装置及其方法 |
CN201610191848.6A Active CN105890943B (zh) | 2014-04-23 | 2014-04-23 | 具有自动检测功能的细胞蜡块制备装置 |
Country Status (1)
Country | Link |
---|---|
CN (8) | CN105928757B (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104324668B (zh) * | 2014-11-26 | 2016-08-31 | 乐卫敏 | 一种组织包埋机熔蜡装置 |
CN106644635A (zh) * | 2016-12-07 | 2017-05-10 | 贵州大学 | 一种石蜡切片自动换药装置及换药方法 |
CN108760447A (zh) * | 2018-05-23 | 2018-11-06 | 深圳大学 | 半自动染色装置 |
CN113390692B (zh) * | 2021-05-24 | 2023-02-28 | 杭州电子科技大学 | 一种用于自动制备细胞蜡块的离心试管 |
CN113390699B (zh) * | 2021-05-24 | 2022-12-09 | 杭州电子科技大学 | 一种冲压式制备细胞蜡块的装置及其方法 |
CN113390700B (zh) * | 2021-05-24 | 2022-12-23 | 杭州电子科技大学 | 一种基于离心法的细胞蜡块自动制备系统及其方法 |
TWI777599B (zh) * | 2021-06-04 | 2022-09-11 | 國立中興大學 | 細胞抓取裝置 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004041994A1 (en) * | 2002-10-31 | 2004-05-21 | University Of Massachusetts | Rapid cell block embedding method and apparatus |
CN101144760A (zh) * | 2007-04-23 | 2008-03-19 | 中国农业大学 | 一种卵母细胞石蜡切片的制备方法 |
CN101344467A (zh) * | 2008-08-20 | 2009-01-14 | 赵晓辉 | 生物组织样本常规石蜡制片he染色无二甲苯全程处理液 |
EP2623950A1 (en) * | 2006-08-15 | 2013-08-07 | Cytyc Corporation | Cell block embedding system and methods |
JP2015501424A (ja) * | 2011-10-18 | 2015-01-15 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 生体試料を収集するための医療装置および方法 |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4074058B2 (ja) * | 1997-11-04 | 2008-04-09 | モノジェン,インコーポレイテッド | 流体試料と粒子物質を混合および流体試料から粒子物質を分離をする方法、およびその装置 |
CN1253716C (zh) * | 2004-01-08 | 2006-04-26 | 中南大学湘雅医学院肿瘤研究所 | 一种用于肿瘤早期诊断的组织芯片及制备器具 |
CN100451649C (zh) * | 2004-01-08 | 2009-01-14 | 中南大学湘雅医学院肿瘤研究所 | 一种细胞芯片制作方法及其器具 |
US20060121597A1 (en) * | 2004-11-24 | 2006-06-08 | Rongshan Li | Cytoblock preparation system and methods of use |
CN100460850C (zh) * | 2005-07-20 | 2009-02-11 | 徐华 | 体液细胞块石蜡切片制作 |
WO2007109639A2 (en) * | 2006-03-20 | 2007-09-27 | Rongshan Li | Cytoblock preparation system and methods of use |
CN201413267Y (zh) * | 2009-05-20 | 2010-02-24 | 赵晓辉 | 生物组织石蜡切片he染色用8缸自动染色机 |
CN101992136B (zh) * | 2010-09-30 | 2012-09-05 | 何洪敏 | 脱落细胞制备细胞块蜡块用组合式离心管 |
CN201841020U (zh) * | 2010-10-26 | 2011-05-25 | 徐海苗 | 一种用于制作细胞蜡块的过滤式塑形离心管 |
EP2458387A3 (en) * | 2010-11-30 | 2013-09-18 | Kabushiki Kaisha Yaskawa Denki | specimen processing system |
CN102764133A (zh) * | 2012-08-10 | 2012-11-07 | 上海科华检验医学产品有限公司 | 一种可直接分离血浆的真空采血管及其方法 |
CN103543057B (zh) * | 2013-11-13 | 2015-12-02 | 何鑫 | 用于微量脱落细胞制备细胞学蜡块用的组合式离心管 |
CN203908836U (zh) * | 2014-04-23 | 2014-10-29 | 杭州电子科技大学 | 自动制备细胞蜡块的装置 |
-
2014
- 2014-04-23 CN CN201610254620.7A patent/CN105928757B/zh active Active
- 2014-04-23 CN CN201610204152.2A patent/CN105890944B/zh not_active Expired - Fee Related
- 2014-04-23 CN CN201610172989.3A patent/CN105628477B/zh not_active Expired - Fee Related
- 2014-04-23 CN CN201610182572.5A patent/CN105865864B/zh active Active
- 2014-04-23 CN CN201610186890.9A patent/CN105842028B/zh active Active
- 2014-04-23 CN CN201610187761.1A patent/CN105865888B/zh active Active
- 2014-04-23 CN CN201410166957.3A patent/CN103969096B/zh active Active
- 2014-04-23 CN CN201610191848.6A patent/CN105890943B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004041994A1 (en) * | 2002-10-31 | 2004-05-21 | University Of Massachusetts | Rapid cell block embedding method and apparatus |
EP2623950A1 (en) * | 2006-08-15 | 2013-08-07 | Cytyc Corporation | Cell block embedding system and methods |
CN101144760A (zh) * | 2007-04-23 | 2008-03-19 | 中国农业大学 | 一种卵母细胞石蜡切片的制备方法 |
CN101344467A (zh) * | 2008-08-20 | 2009-01-14 | 赵晓辉 | 生物组织样本常规石蜡制片he染色无二甲苯全程处理液 |
JP2015501424A (ja) * | 2011-10-18 | 2015-01-15 | ザ・トラスティーズ・オブ・コロンビア・ユニバーシティ・イン・ザ・シティ・オブ・ニューヨーク | 生体試料を収集するための医療装置および方法 |
Also Published As
Publication number | Publication date |
---|---|
CN105890943A (zh) | 2016-08-24 |
CN105890944B (zh) | 2019-01-25 |
CN105928757B (zh) | 2019-01-25 |
CN105865888A (zh) | 2016-08-17 |
CN105842028A (zh) | 2016-08-10 |
CN105865864A (zh) | 2016-08-17 |
CN103969096A (zh) | 2014-08-06 |
CN105865864B (zh) | 2019-01-25 |
CN105890943B (zh) | 2019-01-25 |
CN105628477B (zh) | 2018-01-16 |
CN105628477A (zh) | 2016-06-01 |
CN105890944A (zh) | 2016-08-24 |
CN103969096B (zh) | 2016-06-01 |
CN105928757A (zh) | 2016-09-07 |
CN105842028B (zh) | 2019-02-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105865888B (zh) | 细胞蜡块的制备系统 | |
CN103940658A (zh) | 一种组织细胞标本石蜡包埋的制作方法 | |
CN103759994B (zh) | 一种昆虫幼虫石蜡切片方法 | |
CN104330056B (zh) | 一种准确测量单根蚕丝横截面积的方法及其应用 | |
PT1563055E (pt) | Método de dispositivo para rápido embebimento do blocos de células | |
EP2694942B1 (en) | Method for preparing cytological specimens for analyses | |
CN106501057A (zh) | 一种细胞标本石蜡包埋的改良技术 | |
Wolfe | Tissue processing | |
JP2019532809A (ja) | 中空糸膜の透過特性を決定する方法 | |
CN109738249A (zh) | 一种观察花芽分化过程的石蜡切片的制作方法 | |
CN105699153B (zh) | 自动式细胞蜡块制作设备 | |
CN105675373B (zh) | 具有快速定位功能的细胞蜡块制备装置 | |
CN203908836U (zh) | 自动制备细胞蜡块的装置 | |
CN103954486A (zh) | 制备细胞蜡块的加压式反应装置 | |
CN106706389B (zh) | 岩黄连根的石蜡切片方法 | |
CN203908835U (zh) | 制备细胞蜡块的加压式反应装置 | |
CN203930200U (zh) | 显微镜观察用医疗计数片 | |
CN115165520A (zh) | 一种应用凝胶高效富集微量细胞制备细胞蜡块的方法 | |
CN107976353B (zh) | 作物幼嫩根系石蜡切片的制备方法 | |
CN116907956A (zh) | 一种细胞块制作的方法 | |
CN107576551B (zh) | 脱落细胞在冰冻切片中的制备方法 | |
CN113390699B (zh) | 一种冲压式制备细胞蜡块的装置及其方法 | |
CN113390700B (zh) | 一种基于离心法的细胞蜡块自动制备系统及其方法 | |
CN102636377A (zh) | 病理标本的处理方法 | |
CN208661181U (zh) | 用于血液标本凝固的加热装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
EE01 | Entry into force of recordation of patent licensing contract | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20160817 Assignee: Shenzhen city yinte Mai Technology Co.,Ltd. Assignor: HANGZHOU DIANZI University Contract record no.: X2021330000050 Denomination of invention: Preparation system of cell wax block Granted publication date: 20190222 License type: Common License Record date: 20210616 |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220803 Address after: Room 401, 403, 405, 4th Floor, Building 2, No. 353, Shaoxing Road, Gongshu District, Hangzhou City, Zhejiang Province 310000 Patentee after: Hangzhou SuperSafety Co.,Ltd. Address before: 310018 No. 2 street, Xiasha Higher Education Zone, Hangzhou, Jianggan District, Zhejiang Patentee before: HANGZHOU DIANZI University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240115 Address after: Room 201-5, Building 2, Jinghu Dianjin Business Plaza, No. 101 Nanjiao Shuyuan Road, Chengxiang Town, Taicang City, Suzhou City, Jiangsu Province, 215400 Patentee after: Suzhou Teerxin Medical Technology Co.,Ltd. Address before: Room 401, 403, 405, 4th Floor, Building 2, No. 353, Shaoxing Road, Gongshu District, Hangzhou City, Zhejiang Province 310000 Patentee before: Hangzhou SuperSafety Co.,Ltd. |