CN105861352A - Enterobacter ludwigii with quinclorac degradation function and application thereof - Google Patents
Enterobacter ludwigii with quinclorac degradation function and application thereof Download PDFInfo
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- CN105861352A CN105861352A CN201510032808.2A CN201510032808A CN105861352A CN 105861352 A CN105861352 A CN 105861352A CN 201510032808 A CN201510032808 A CN 201510032808A CN 105861352 A CN105861352 A CN 105861352A
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- quinolinic acid
- enterobacteria
- dichloro quinolinic
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Abstract
The present invention discloses Enterobacter ludwigii with preservation No. CGMCC No. 10128. The invention also discloses a composition containing the Enterobacter ludwigii live bacteria described above. The present invention further discloses the application of above Enterobacter ludwigii and compositions in the degradation of quinclorac. The present invention also discloses a method for degrading quinclorac. The method comprises the steps of: under the conditions suitable for Enterobacter ludwigii to survive, adding the above Enterobacter ludwigii or composition into an environment containing quinclorac in order to degrade quinclorac. The Enterobacter ludwigii simply requires the conditions of carbon source, nitrogen source and inorganic salts to survive and grow in an atmosphere containing quinclorac, has the advantages of strong vitality, fast growth, large biomass and high tolerance and degradation ability to quinclorac.
Description
Technical field
The present invention relates to a strain Ludwig enterobacteria (Enterobacter ludwigii), in particular it relates to
One strain has Ludwig enterobacteria and the application thereof of dichloro quinolinic acid degradation function, and containing this rood
The compositions of Vichy enterobacteria and the method for degraded dichloro quinolinic acid.
Background technology
Dichloro quinolinic acid (the chloro-8-Quinoline Carboxylic Acid of 3,7-bis-) is a kind of Gao Xuan of BASF Corp. of Germany's exploitation
The hormone like herbicide of selecting property, is widely used for controlling unifacial leaf and dicotyledonous in many Rice Cropping countries
Weeds, have good herbicidal effect and more long residual phase particularly with preventing and kill off barnyard grass in paddy field, and application is increasingly
Extensively.Owing to some users lack enough understanding to the weeding characteristic of dichloro quinolinic acid, blindly apply and increase
Dosage, thus cause pedo relict that second stubble crop produces poisoning, dichloro quinolinic acid is used in latter 30 days
Any crop can not be planted in addition to Oryza sativa L., can not grow tobacco within 1 year, Fructus Solani melongenae, can not plant in 2 years kind
Eggplant, Radix Dauci Sativae.Additionally, the Carrot family crop such as Herba Apii graveolentis, Herba Coriandri is the most sensitive to dichloro quinolinic acid, no
The available water executing dichloro quinolinic acid waters above-mentioned crop.The wherein dichloro quinolinic acid teratogenesis symptom to Nicotiana tabacum L.
The most obvious, occur mainly in Tobacco-rice rotation area, and occur in whole field sheet.Therefore, in soil with
Dichloro quinolinic acid is that the main residual growth for flue-cured tobacco of agriculture has harmful effect with quality, can be to leaf tobacco production band
Carry out serious economic loss.Sum it up, agriculture based on dichloro quinolinic acid residual in soil the residence time
Long, rear stubble tobacco planting can be produced serious harm, key urgently to be resolved hurrily during becoming tobacco planting
Technical problem, wherein key is that the soil to residual dichloro quinolinic acid is repaired.Therefore, dichloro
Quinolinic acid herbicide carryover and its pollution on the environment are problem demanding prompt solutions in current leaf tobacco production.
Summary of the invention
The invention aims to overcome drawbacks described above, it is provided that a strain Ludwig enterobacteria
(Enterobacter ludwigii), notable with degradation effect, simple to operate, cheap and environmental friendliness
Mode dichloro quinolinic acid is degraded.
To achieve these goals, on the one hand, the invention provides a kind of Ludwig enterobacteria
(Enterobacter ludwigii), wherein, the deposit number of described Ludwig enterobacteria is CGMCC
No.10128, named LZ-1.
Second aspect, the invention provides a kind of compositions, and wherein, said composition contains as above
The viable bacteria body of Ludwig enterobacteria (Enterobacter ludwigii).
The third aspect, the invention provides Ludwig enterobacteria as above at degraded dichloro quinolinic acid
In application.
Fourth aspect, a kind of method that the invention provides dichloro quinolinic acid of degrading, the method includes:
Under conditions of Ludwig enterobacteria can survive, by Ludwig enterobacteria as above
(Enterobacter ludwigii), and/or compositions joins containing dichloro quinolinic acid as above
In environment, so that dichloro quinolinic acid is degraded.
Preferably, the method also includes, during to described dichloroquinoline acid degradation, contains to described
There is interpolation inorganic salt in the environment of dichloro quinolinic acid.It is furthermore preferred that described inorganic salt is KH2PO4,
K2HPO4, NH4NO3, MgSO4, CaCl2And FeSO4。
Preferably, in the described environment containing dichloro quinolinic acid, Ludwig intestinal bar as above is added
Bacterium (Enterobacter ludwigii), the described Ludwig enterobacteria of addition is by through liquid culture solid-liquid
Solid phase after separation and/or the bacterium colony after solid culture provide.
Ludwig enterobacteria of the present invention (Enterobacter ludwigii) only need to be provided with carbon
Under conditions of source, nitrogen source and inorganic salt, can survive in the culture environment containing dichloro quinolinic acid and grow,
Vitality is strong, fast growth, and Biomass is big, has the highest toleration and degraded energy to dichloro quinolinic acid
Power.
Other features and advantages of the present invention will be described in detail in detailed description of the invention part subsequently.
Biological deposits
The bacterial strain of the present invention is named as Ludwig enterobacteria (Enterobacter ludwigii), and in
Within 2014, December is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on the 2nd
(address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postal
Political affairs encode: 100101) (depositary institution is abbreviated as CGMCC), deposit number is CGMCC No.
10128, named LZ-1.
Detailed description of the invention
Hereinafter the detailed description of the invention of the present invention is described in detail.It should be appreciated that this place is retouched
The detailed description of the invention stated is merely to illustrate and explains the present invention, is not limited to the present invention.
First aspect, the invention provides a kind of Ludwig enterobacteria (Enterobacter ludwigii),
Wherein, the deposit number of described Ludwig enterobacteria is CGMCC No.10128.
The Ludwig enterobacteria (Enterobacter ludwigii) of the present invention is isolatable from Xuancheng Profile, anhui Province by two
The cigarette that chloro-quinolinic acid pollutes plants soil.
The Ludwig enterobacteria that the present invention provides can produce a large amount of Ludwig enterobacteria through cultivation
Viable bacteria body, the particularly requirement of the method for described cultivation, as long as described Ludwig intestinal bar can be made
Bacterium breeds, such as can be according to 107The inoculum concentration of CFU/mL is by the viable bacteria of Ludwig enterobacteria
Body is inoculated in LB culture medium, and under aerobic condition, cultivates 8-72 at a temperature of 25-38 DEG C
After hour, obtain culture fluid.
The present invention can separate the viable bacteria body of the Ludwig enterobacteria in above-mentioned culture fluid further, described
The method separated has no particular limits, as long as thalline can be enriched with from culture fluid, the most permissible
Being realized by method that is centrifugal and/or that filter, described centrifugal and described filtration condition can be known bar
Part, the present invention does not repeats them here.
The 16s rDNA's of the Ludwig enterobacteria (Enterobacter ludwigii) that the present invention provides
Nucleotide sequence is as shown in SEQ ID No:1.
Second aspect, the invention provides a kind of compositions, and wherein, said composition contains as above
The viable bacteria body of Ludwig enterobacteria (Enterobacter ludwigii).
According to the present invention, in the composition, the concentration of described Ludwig enterobacteria is the most particularly
Limiting, can carry out concrete selection according to specific circumstances, in this not go into detail.
It addition, different according to intended purpose, the compositions that the present invention provides can be prepared as different dosage forms,
And it is added with the excipient etc. that the activity of described Ludwig enterobacteria will not be impacted accordingly
Composition.Concrete being chosen as is known to one of skill in the art, and in this not go into detail for the present invention.
The third aspect, present invention also offers Ludwig enterobacteria as above and/or as mentioned above
Compositions degraded dichloro quinolinic acid in application.
There is carbon source, nitrogen source and the condition of inorganic salt having only in the Ludwig enterobacteria that the present invention provides
Under, can survive in the culture environment containing dichloro quinolinic acid and grow, vitality is strong, fast growth,
Biomass is big, has the highest toleration and degradation capability to dichloro quinolinic acid.
Fourth aspect, a kind of method that the invention provides dichloro quinolinic acid of degrading, the method includes:
Under conditions of Ludwig enterobacteria can survive, by Ludwig enterobacteria as above
(Enterobacter ludwigii), or compositions joins the environment containing dichloro quinolinic acid as above
In, so that dichloro quinolinic acid is degraded.
According to the present invention, term " Ludwig enterobacteria can survive " refers to containing dichloro quinolinic acid
Environment in, at least about 20%, preferably at least more than 40%, the more preferably at least bacterium more than 60%
Body can be survived.Term " condition that Ludwig enterobacteria can survive " refer at least to include carbon source,
Nitrogen source and the condition being able to maintain that thalline vitality of inorganic salt.
According to the present invention, the described environment containing dichloroquinoline acid pollution can include any containing two chloroquines
The environment of quinoline acid, such as, the described environment containing dichloroquinoline acid pollution can include containing dichloroquinoline
The soil of acid or water body.
According to the present invention, it is seeded to the Ludwig intestinal bar in the described environment containing dichloroquinoline acid pollution
The form of bacterium is not particularly limited, as long as described Ludwig enterobacteria can be in institute after ensureing inoculation
State and the environment containing dichloroquinoline acid pollution works and described dichloro quinolinic acid is degraded effectively
, the form of the described Ludwig enterobacteria of inoculation, for example, it is possible to for cultivating the work to logarithmic (log) phase
Change thalline, it is also possible to for the thalline dry powder after lyophilization, be preferably and cultivate the activation thalline to logarithmic (log) phase.
The Ludwig enterobacteria quantity of inoculation is also had no particular limits by the present invention, and this can be according to institute
State the content of dichloro quinolinic acid in the environment containing dichloroquinoline acid pollution and bacterial strain at described environment
In survival ability determine, such as, when the dichloroquinoline acid content in described environment is higher or described ring
Border for the existence of described Ludwig enterobacteria less favorable time, described Ludwig enterobacteria can be improved
Inoculum concentration；When the dichloroquinoline acid content in described environment is relatively low or described environment is to described Ludwig
When the survival effect of enterobacteria is less, it is possible to reduce the inoculum concentration of described Ludwig enterobacteria.
The content of dichloro quinolinic acid in the described environment containing dichloroquinoline acid pollution is not had by the present invention yet
There is particularly restriction, but consider described Ludwig enterobacteria containing in dichloroquinoline acid pollution
Survival ability and the degradation effect to dichloro quinolinic acid, relative to containing dichloro quinolinic acid described in every kilogram
The environment polluted, the content of described dichloro quinolinic acid is not higher than 200mg, preferably no greater than 100mg,
More preferably no higher than 50mg.
It addition, the present invention is it should be noted that working as the described environment containing dichloroquinoline acid pollution is water body
Time, what in the environment of dichloroquinoline acid pollution, the content of dichloro quinolinic acid can also approximate be considered relative to
Containing the environment of dichloroquinoline acid pollution described in every liter, the content of described dichloro quinolinic acid is not higher than 200mg,
It is preferably no greater than 100mg, more preferably no higher than 50mg.
According to the present invention, in order to improve the Ludwig enterobacteria of present invention offer further containing dichloro
Survival ability in the environment that quinolinic acid pollutes and activity, the method for the present invention also includes to described environment
The inorganic salt that middle offer is extra.
Wherein, the kind of described extra inorganic salt can be known in the art for cultivating Ludwig
The kind of the inorganic salt of enterobacteria.Preferably, described inorganic salt is KH2PO4, K2HPO4, NH4NO3,
MgSO4, CaCl2And FeSO4In one or more, more preferably KH2PO4, K2HPO4, NH4NO3,
MgSO4, CaCl2And FeSO4。
Wherein, the amount of the present invention described inorganic salt to adding has no particular limits, can be according to described
In environment containing dichloroquinoline acid pollution depending on the kind of inorganic salt and content.Such as, with KH2PO4
(0.8-1.2%), K2HPO4(0.8-1.2%), NH4NO3(1-1.5%), MgSO4(0.03-0.08%),
CaCl2(0.001-0.003%), FeSO4·7H2As a example by the mixed inorganic salt solution of O (0.01-0.03%),
Based on described in every kilogram containing the environment of dichloroquinoline acid pollution, the addition of above-mentioned inorganic salt mixed liquor
It can be 1-3ml/ days.
According to the present invention, when the described environment containing dichloro quinolinic acid is soil, in order to promote further
The Ludwig enterobacteria that the present invention the provides degradation efficiency to dichloro quinolinic acid, it is preferred that by soil
Water content control at least 15 weight %, more preferably 18-30 weight %.
It addition, in the present invention, as above when adding in the described environment containing dichloro quinolinic acid
During Ludwig enterobacteria (Enterobacter ludwigii), it is preferably added to described Ludwig intestinal bar
Bacterium is by the solid phase after liquid culture solid-liquid separation and/or the bacterium colony after solid culture, preferred at this
In the case of, enterobacteria is wished to the degradation effect of dichloro quinolinic acid more preferably in the Devi that the present invention provides.
According to the present invention, described Ludwig enterobacteria solid phase after liquid culture solid-liquid separation is the most excellent
Choosing uses deionized water to wash.
Hereinafter will be described the present invention by embodiment.
In following example:
LB fluid medium: 0.8-1% peptone, 0.5-0.8% yeast powder, 1-1.5% sodium chloride,
PH=6.8-7.0.
Minimal medium: KH2PO4(0.8-1.2%), K2HPO4(0.8-1.2%), NH4NO3
(1-1.5%), MgSO4(0.03-0.08%), CaCl2(0.001-0.003%), FeSO4·7H2O
(0.01-0.03%), pH=6.0-7.5.
The bacterial strain of the present invention is Ludwig enterobacteria (Enterobacter ludwigii), hereinafter referred to as
LZ-1, and it is common within 2nd, to be deposited in China Committee for Culture Collection of Microorganisms in December in 2014
(address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microorganism, microorganism center
Institute, postcode: 100101) (depositary institution is abbreviated as CGMCC), deposit number is
CGMCC No.10128.The activation of bacterial strain is carried out in LB fluid medium.
After bacterial strain LZ-1 is inoculated with the inoculum concentration of 1-2 volume % respectively in LB culture medium,
25-35 DEG C, cultivate under conditions of 150-240rpm 18 hours standby.
Embodiment 1
The present embodiment for illustrate Ludwig enterobacteria that the present invention provides in water to dichloro quinolinic acid
Toleration and degradability
It is respectively 10mg/L containing concentration after bacterial strain LZ-1 is accessed sterilizing with the inoculum concentration of 1 volume %,
In the LB fluid medium of 20mg/L, 50mg/L, 100mg/L, 200mg/L dichloro quinolinic acid.?
170rpm, cultivates 24h under the conditions of 30 ± 1 DEG C, result shows, dichloro quinolinic acid is shown by bacterial strain LZ-1
Having shown good toleration, in different culture media, bacterial strain can grow.The maximum OD that thalline can reach
Value and the degradation rate of dichloro quinolinic acid is shown in Table 1.
Embodiment 2
The present embodiment for illustrate Ludwig enterobacteria that the present invention provides in water to dichloro quinolinic acid
Toleration and degradability
It is respectively 10mg/L containing concentration after bacterial strain LZ-1 is accessed sterilizing with the inoculum concentration of 1 volume %,
In the minimal medium of the dichloro quinolinic acid of 20mg/L, 50mg/L, 100mg/L, 200mg/L.?
170rpm, cultivates 24h under the conditions of 30 ± 1 DEG C.Result shows, dichloro quinolinic acid is shown by bacterial strain LZ-1
Having shown good toleration, in different culture media, bacterial strain can grow.It is being unique with dichloro quinolinic acid
In the case of carbon source, maximum OD value that thalline can reach and the degradation rate of dichloro quinolinic acid is shown in Table 1.
Comparative example 1-2
The present embodiment is for illustrating under naturalness the degradability of dichloro quinolinic acid in water
Test according to the method for embodiment 1-2 respectively, except for the difference that, do not inoculate
Any bacterial strain.The results are shown in Table 1.
Table 1
As can be seen from Table 1, dichloro quinolinic acid is had in water by the Ludwig enterobacteria that the present invention provides
There is stronger toleration, and living environment is required loose, be provided only with dichloro quinolinic acid as carbon
The minimal medium in source also is able to grow, and dichloro quinolinic acid is effectively degraded.And
And, in the case of being preferably added to inorganic salt, the Ludwig enterobacteria that the present invention provides is to dichloroquinoline
The degradation rate of acid can be further enhanced.
Embodiment 3
The present embodiment for illustrate Ludwig enterobacteria that the present invention provides in soil to dichloroquinoline
The degradability of acid
Bacterial strain LZ-1 is inoculated in the LB fluid medium of 100mL with 1 volume % strain amount, 175
Rmp, after cultivating 24h, joins the dichloroquinoline that 5kg contains 20mg/kg at 30 ± 1 DEG C by bacterium solution
In the contaminated soil of acid, stir, cultivate 7 days, it is ensured that be about 20% containing the water yield in soil,
Finally the content of dichloro quinolinic acid in detection soil, the results are shown in Table 2.
Embodiment 4
The present embodiment for illustrate Ludwig enterobacteria that the present invention provides in soil to dichloroquinoline
The degradability of acid
Bacterial strain LZ-1 is inoculated in the LB fluid medium of 100mL with 1 volume % strain amount, 175
Rmp, cultivates after 24h at 30 ± 1 DEG C, takes 1mL bacterium solution and joins 5kg and contain the two of 20mg/kg
In the contaminated soil of chloro-quinolinic acid, stir, and add 10mL minimal medium every day, cultivate
7 days, it is ensured that be about 20% containing the water yield in soil, the finally content of dichloro quinolinic acid in detection soil,
The results are shown in Table 2.
Embodiment 5
The present embodiment for illustrate Ludwig enterobacteria that the present invention provides in soil to dichloroquinoline
The degradability of acid
Bacterial strain LZ-1 is inoculated in the LB fluid medium of 100mL with 1 volume % strain amount, 175
Rmp, cultivates after 24h at 30 ± 1 DEG C, bacterium solution is centrifuged, and removes supernatant, and thalline is spent from
Sub-water washs in the contaminated soil joining the dichloro quinolinic acid containing 20mg/kg containing 5kg after 3 times,
Stir, cultivate 7 days, it is ensured that be about 20% containing the water yield in soil, finally in detection soil two
The content of chloro-quinolinic acid, the results are shown in Table 2.
Comparative example 3-5
The present embodiment is for illustrating under nature shape the degradability of dichloro quinolinic acid in soil
Test according to the method for embodiment 3-5 respectively, except for the difference that, the most do not inoculate in soil and appoint
What bacterial strain.The results are shown in Table 2.
Table 2
As can be seen from Table 2, the Ludwig enterobacteria that the present invention provides is to the dichloro quinolinic acid in soil
Can effectively degrade.It addition, be preferably added to inorganic salt or joining soil with pure thalline form
In the case of in earth, the degradation rate of dichloro quinolinic acid can be obtained by the Ludwig enterobacteria that the present invention provides
To improving further.
The preferred embodiment of the present invention described in detail above, but, the present invention is not limited to above-mentioned reality
Execute the detail in mode, in the technology concept of the present invention, can be to the technical side of the present invention
Case carries out multiple simple variant, and these simple variant belong to protection scope of the present invention.
It is further to note that each the concrete technical characteristic described in above-mentioned detailed description of the invention,
In the case of reconcilable, can be combined by any suitable means.In order to avoid unnecessary
Repeating, various possible compound modes are illustrated by the present invention the most separately.
Additionally, combination in any can also be carried out between the various different embodiment of the present invention, as long as its
Without prejudice to the thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a Ludwig enterobacteria (Enterobacter ludwigii), it is characterised in that described road
It is CGMCC No.10128 that the deposit number of enterobacteria is wished in Devi.
2. a compositions, it is characterised in that said composition contains the rood dimension described in claim 1
The viable bacteria body of uncommon enterobacteria (Enterobacter ludwigii).
3. the Ludwig enterobacteria described in claim 1, the compositions described in claim 2 are in fall
Solve the application in dichloro quinolinic acid.
4. degrading the method for dichloro quinolinic acid, the method includes: can Ludwig enterobacteria
Under conditions of survival, by the Ludwig enterobacteria (Enterobacter ludwigii) described in claim 1,
And/or the compositions described in claim 2 joins in the environment containing dichloro quinolinic acid, with to two chloroquines
Quinoline acid is degraded.
Method the most according to claim 4, wherein, the described environment bag containing dichloro quinolinic acid
Include soil or water body.
Method the most according to claim 4, wherein, the method also includes, to described dichloro
During quinolinic acid degraded, in the described environment containing dichloro quinolinic acid, add inorganic salt.
Method the most according to claim 6, wherein, described inorganic salt is KH2PO4, K2HPO4,
NH4NO3, MgSO4, CaCl2And FeSO4In one or more.
Method the most according to claim 6, wherein, described inorganic salt is KH2PO4, K2HPO4,
NH4NO3, MgSO4, CaCl2And FeSO4。
9. according to the method described in claim 4 or 5, wherein, relative to containing two described in every kilogram
The environment of chloro-quinolinic acid, the content of described dichloro quinolinic acid is not higher than 200mg.
10. according to the method described in any one in claim 4-8, wherein, to described containing dichloro
The environment of quinolinic acid adds the Ludwig enterobacteria (Enterobacter described in claim 1
Ludwigii), the described Ludwig enterobacteria of addition by the solid phase after liquid culture solid-liquid separation and/
Or the bacterium colony after solid culture provides.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510032808.2A CN105861352B (en) | 2015-01-22 | 2015-01-22 | With the Ludwig enterobacteria of dichloro quinolinic acid degradation function and its application |
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| CN201510032808.2A CN105861352B (en) | 2015-01-22 | 2015-01-22 | With the Ludwig enterobacteria of dichloro quinolinic acid degradation function and its application |
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| CN105861352B CN105861352B (en) | 2019-08-16 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520619A (en) * | 2016-11-15 | 2017-03-22 | 山东科技大学 | Enterobacter ludwigii and application of same to induction of magnesium ion mineralization |
| CN107629980A (en) * | 2017-09-30 | 2018-01-26 | 中南民族大学 | The Ludwig enterobacteria SXZ N5 bacterial strains and purposes of one plant of production huperzine and Huperzine B |
| CN114806952A (en) * | 2022-05-09 | 2022-07-29 | 扬州大学附属医院 | Enterobacter ludwigii and application thereof |
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|---|---|---|---|---|
| CN101089121A (en) * | 2006-06-16 | 2007-12-19 | 河南农业大学 | Application of whiterof fungus prepn in improving the continuous cropping performance of soil |
| CN103243060A (en) * | 2013-05-27 | 2013-08-14 | 湖南农业大学 | Quinclorac degrading bacteria and application thereof |
| CN103849589A (en) * | 2014-03-17 | 2014-06-11 | 中国农业科学院烟草研究所 | High-efficiency degradation strain for quinclorac and applications and using method thereof |
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2015
- 2015-01-22 CN CN201510032808.2A patent/CN105861352B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101089121A (en) * | 2006-06-16 | 2007-12-19 | 河南农业大学 | Application of whiterof fungus prepn in improving the continuous cropping performance of soil |
| CN103243060A (en) * | 2013-05-27 | 2013-08-14 | 湖南农业大学 | Quinclorac degrading bacteria and application thereof |
| CN103849589A (en) * | 2014-03-17 | 2014-06-11 | 中国农业科学院烟草研究所 | High-efficiency degradation strain for quinclorac and applications and using method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520619A (en) * | 2016-11-15 | 2017-03-22 | 山东科技大学 | Enterobacter ludwigii and application of same to induction of magnesium ion mineralization |
| CN107629980A (en) * | 2017-09-30 | 2018-01-26 | 中南民族大学 | The Ludwig enterobacteria SXZ N5 bacterial strains and purposes of one plant of production huperzine and Huperzine B |
| CN114806952A (en) * | 2022-05-09 | 2022-07-29 | 扬州大学附属医院 | Enterobacter ludwigii and application thereof |
| CN114806952B (en) * | 2022-05-09 | 2023-05-16 | 扬州大学附属医院 | Ledeb Vichis enterobacteria and application thereof |
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| Publication number | Publication date |
|---|---|
| CN105861352B (en) | 2019-08-16 |
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