CN105851352A - Effective part composition containing white tea extracts and preparation method and application of effective part composition - Google Patents

Effective part composition containing white tea extracts and preparation method and application of effective part composition Download PDF

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Publication number
CN105851352A
CN105851352A CN201610428974.9A CN201610428974A CN105851352A CN 105851352 A CN105851352 A CN 105851352A CN 201610428974 A CN201610428974 A CN 201610428974A CN 105851352 A CN105851352 A CN 105851352A
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powder
extract
ramulus
mussaendae pubescentis
preparation
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秦艳
王娜
唐惠奇
康林芝
云帆
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Guangzhou Alchem Biotechnology Co Ltd
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Guangzhou Alchem Biotechnology Co Ltd
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Priority to CN201610428974.9A priority Critical patent/CN105851352A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea

Abstract

The invention discloses an effective part composition containing white tea extracts and a preparation method and application of the effective part composition. The effective part composition is formed by treating the white tea extracts as the main component and adding cyclocarya paliurus powder, folium mori powder, fructus crataegi powder, grape seed extracts, tea polyphenol and the like. The raw materials which are medicinal and edible natural plant resources and plant effective part extracts can be prepared into healthcare products of various dosage forms, the effective part composition has various healthcare functions of resisting oxidation and ageing, reducing blood pressure and blood glucose, resisting radiation and tumor and the like, the use range of white tea type products is brought into play to a larger extent, and the effective part composition is suitable for application and popularization.

Description

A kind of active component composition containing Ramulus et Folium Mussaendae Pubescentis extract and its preparation method and application
Technical field
The present invention relates to containing Ramulus et Folium Mussaendae Pubescentis extract application in food or health product, be specifically related to a kind of with in vain Tea extract is main component, and the active component composition coordinating the component of other several integration of edible and medicinal herbs to obtain exists Application in food or health product.
Background technology
Ramulus et Folium Mussaendae Pubescentis is one of Chinese six great tradition teas, belongs to micro-fermented tea, completely drapes over one's shoulders pekoe because of it, if silver is like snow And gain the name, its cradle is in Fuding City of Fujian Province of China, and its product soup color is light;Flavour is fresh pure, fresh and sweet; Fragrance is elegant, has milli fragrant;At the bottom of leaf tender even.Ramulus et Folium Mussaendae Pubescentis free-radical contents is low, rich in tea polyphenols, aminoacid, yellow Ketone content is high, research show Ramulus et Folium Mussaendae Pubescentis have notable blood fat reducing, blood sugar lowering, blood pressure lowering, antioxidation, radioprotective, The effects such as antitumor.Therefore, inquire into the exploitation of Ramulus et Folium Mussaendae Pubescentis product, extend its industrial chain, it is achieved Ramulus et Folium Mussaendae Pubescentis industrialization Have great importance.
At present, marketed Ramulus et Folium Mussaendae Pubescentis health product, product quantity is few, and product category is single, and supports from the traditional Chinese medical science Raw considering, single Ramulus et Folium Mussaendae Pubescentis health promoting product exists that composition is simple, functional component is comprehensive, and health-care effect does not shows The shortcomings such as work.
Summary of the invention
It is an object of the invention to the health product above shortcomings according to existing Ramulus et Folium Mussaendae Pubescentis class, it is provided that a kind of New with Ramulus et Folium Mussaendae Pubescentis extract as primary raw material, add the natural of other several integration of edible and medicinal herbs according to special ratios and plant Thing effective part extract, obtained active component composition can be made the health product of various dosage form, have There are the various health care functions such as antioxidation, defying age, blood pressure lowering, blood sugar lowering, radioprotective, antitumor, more greatly The range having played Ramulus et Folium Mussaendae Pubescentis series products of limit.
Another object of the present invention is to provide the preparation side of the above-mentioned active component composition containing Ramulus et Folium Mussaendae Pubescentis extract Method.
It is also an object of the present invention to provide answering of the above-mentioned active component composition containing Ramulus et Folium Mussaendae Pubescentis extract With.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A kind of active component composition containing Ramulus et Folium Mussaendae Pubescentis extract, it is characterised in that described active component composition by Form according to the component of mass percentage as follows: Ramulus et Folium Mussaendae Pubescentis extract 50~60%, Cyclocarya paliurus Iljinskaja powder 5~10%, Mulberry Leaf powder 5~10%, Fructus Crataegi powder 5~10%, Semen Vitis viniferae extract 10~15%, tea polyphenols 10~15%;
Wherein, the preparation method of described Ramulus et Folium Mussaendae Pubescentis extract is: dries Ramulus et Folium Mussaendae Pubescentis to constant weight, then pulverizes and sieves, Extract with ethanol solution, cross leaching filtrate, be dried after removing ethanol, obtain Ramulus et Folium Mussaendae Pubescentis extract;
The preparation method of described Semen Vitis viniferae extract is: dries Semen Vitis viniferae to constant weight, then pulverizes and sieves, Extract with ethanol solution, cross leaching filtrate, be dried after removing ethanol, obtain Semen Vitis viniferae extract;
The preparation method of described Cyclocarya paliurus Iljinskaja powder, powder of Folium Mori and Fructus Crataegi powder is: take blue or green money, Folium Mori, Fructus Crataegi respectively, Dry to constant weight, after pulverizing and sieving, respectively obtain Cyclocarya paliurus Iljinskaja powder, powder of Folium Mori and Fructus Crataegi powder.
As further optimal technical scheme, it is also possible to add Xian Chi Serpentis Portugal on the basis of above-mentioned composition Grape leaf powder, the most described active component composition is by forming according to the component of mass percentage as follows: Ramulus et Folium Mussaendae Pubescentis Extract 40~50%, Ampelopsis grossedentata leaf powder 10~20%, Cyclocarya paliurus Iljinskaja powder 5~10%, powder of Folium Mori 5~10%, Fructus Crataegi powder 5~10%, Semen Vitis viniferae extract 5~10%, tea polyphenols 5~10%;Wherein, described Ampelopsis grossedentata The preparation method of leaf powder is: takes Ampelopsis grossedentata leaf and dries to constant weight, after pulverizing and sieving, obtains Xian Chi Serpentis Portugal Grape leaf powder.
As further optimal technical scheme, in the preparation method of described Ramulus et Folium Mussaendae Pubescentis extract, described drying is 50 DEG C of drying, described in sieve be 80 mesh sieves, the volume fraction of described ethanol solution is 50~80%, extraction Solid-liquid ratio be 1:10~1:20, extraction temperature is 30~60 DEG C, and extraction time is 2~3h, and described being dried is true Vacuum freecing-dry.
As further optimal technical scheme, in the preparation method of described Semen Vitis viniferae extract, described drying 50 DEG C of drying, described in sieve be 80 mesh sieves, the volume fraction of described ethanol solution is 30~50%, leaching The solid-liquid ratio carried is 1:10~1:20, and extraction temperature is 25~60 DEG C, and extraction time is 0.5~2h, described dry For vacuum lyophilization.
As further optimal technical scheme, in the preparation method of described Cyclocarya paliurus Iljinskaja powder powder of Folium Mori and Fructus Crataegi powder, Described drying is 50 DEG C of drying, described in sieve be 80 mesh sieves.
As further optimal technical scheme, in the preparation method of described Ampelopsis grossedentata leaf powder, described baking Dry is 50 DEG C of drying, described in sieve be 80 mesh sieves.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract of the present invention can be prepared as various dosage form as required Health food or health care medicine, for antioxidation, defying age, lowering blood pressure and blood fat, blood sugar lowering, dimension Hold blood pressure stabilization, radioprotective or antitumor.
Compared with prior art, there is advantages that
(1) present invention uses Ampelopsis grossedentata leaf as one of component of active component composition, Xian Chi Serpentis Portugal Grape leaf has the effects such as blood sugar lowering, blood fat reducing and atherosclerosis.Meanwhile, in Ampelopsis grossedentata leaf Containing substantial amounts of flavone compound, it is mainly composed of as dihydromyricetin, and it has higher free radical Scavenging activity, up to 73.3%~91.5%, can significantly alleviate the damage of body internal oxidition, there is the work of defying age With.Therefore, choose Ramulus et Folium Mussaendae Pubescentis extract and Ampelopsis grossedentata leaf compatibility, make compositions remove and there is Ramulus et Folium Mussaendae Pubescentis extract Effect outside, the effect of blood pressure lowering and defying age is the most particularly pertinent;
(2) present invention uses Cyclocarya paliurus Iljinskaja to contain polysaccharide as one of component of active component composition, leaf of Cyclocarya paliurus Iljinskaja, Flavone, the multiple important biomolecule active component such as saponin, simultaneously possibly together with aminoacid, selenium, vanadium, chromium, germanium, Zinc, ferrum, the trace element of the multiple preciousness such as calcium.Research shows, Cyclocarya paliurus Iljinskaja has good regulation blood glucose Health-care effect, it has certain preventive and therapeutic effect, can also effectively reduce sweet simultaneously cardiovascular disease and diabetes Oil three esters and cholesterol level.Therefore choose Ramulus et Folium Mussaendae Pubescentis extract and Cyclocarya paliurus Iljinskaja compatibility, make compositions remove have white Outside effect of tea extract, especially blood sugar lowering there is remarkable result;
(3) containing bioactive ingredients such as polysaccharide, alkaloid and flavone in Folium Mori, it can be thin at reparation islets of langerhans While born of the same parents, providing substantial amounts of nutrient substance, trophocyte, dredging vascellum for cell, treating both the principal and secondary aspects of a disease improves Complication.Research finds, the 1-deoxidation nojirimycin contained in Folium Mori, it can suppress polysaccharide is resolved into Portugal α-sugared sweet the enzymatic activity of grape sugar, thus suppress the rising of blood glucose.Folium Mori, except protecting pancreas, promote islets of langerhans The normal secretions of element, moreover it is possible to improve hypertension, reduces cholesterol and triglyceride.Therefore the present invention chooses white Tea extract and powder of Folium Mori compatibility, make compositions in addition to the effect with Ramulus et Folium Mussaendae Pubescentis extract, enhance blood sugar lowering Effect;
(4) Fructus Crataegi nutrient substance enriches, the particularly bioactive ingredients such as VC, flavone compound Content is high, and the multiple mineral nutrient element containing needed by human body.Fructus Crataegi has higher healthy nutritive value, Have inflammation-diminishing and cough-controlling, promoting digestion and invigorating the stomach, blood pressure lowering, blood fat reducing, enhancement coronary artery blood flow, prevent and treat coronary heart disease, Angina pectoriss etc. act on, and meanwhile, it has stronger antioxidation and scavenging activated oxygen ability, therefore has anti- Cancer, anti-cancer efficacy.The present invention chooses Ramulus et Folium Mussaendae Pubescentis extract and Fructus Crataegi powder compatibility, makes compositions remove and has Ramulus et Folium Mussaendae Pubescentis and carry Take outside effect of thing, also there is antioxidation, blood fat reducing, strengthen the effect of body immunity;
(5) containing abundant antioxidation, the nutritional labeling procyanidin of defying age in Semen Vitis viniferae, have Multiple biological activity, is one of the most efficient anioxidant phytochemicals of having now been found that, and meanwhile, Semen Vitis viniferae extracts Thing also has vasodilator, antihypertensive effect;Semen Vitis viniferae extract has dynamic regulation to immunologic function Effect, increases the cytotoxicity killing cell, strengthens the function of lymphocyte.Therefore the present invention chooses Ramulus et Folium Mussaendae Pubescentis Extract and Semen Vitis viniferae extract compatibility, make compositions in addition to the effect with Ramulus et Folium Mussaendae Pubescentis extract, it is also possible to anti- Oxidation, blood pressure lowering, strengthen body immunity;
(6) tea polyphenols is a kind of excellent Natural antioxidant, is flavanol compound in Folium Camelliae sinensis, anthocyanin class, Huang Ketone, flavonols and the general name of phenolic acids isoreactivity material, it is mainly composed of flavonol (catechin) Class, catechin accounts for 60~80%, tea polyphenols have defying age, have anticancer, remove human free radical, radioprotective, A series of pharmacological functions such as blood pressure lowering, blood sugar lowering, blood fat reducing.Therefore the present invention chooses Ramulus et Folium Mussaendae Pubescentis extract and tea Polyphenol compatibility, make compositions in addition to the effect with Ramulus et Folium Mussaendae Pubescentis extract, also can antioxidation, defying age;
(7) present invention expands the scope of application of Ramulus et Folium Mussaendae Pubescentis, on the basis of the health care that Ramulus et Folium Mussaendae Pubescentis has originally, Through adding the plant extract of multiple integration of edible and medicinal herbs, the compositions of gained can strengthen the health care of Ramulus et Folium Mussaendae Pubescentis periphery Function, gives it and is more worth, and compared with single Ramulus et Folium Mussaendae Pubescentis or other components, the present invention is specific Ratio can make the effect of health care become apparent from;
(8) preparation method of active component composition of the present invention is simple, it is not necessary to special installation, easily real Existing industrialization large-scale production.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but the present invention is not done any by embodiment The restriction of form.
In following example, if no special instructions, the raw material used is raw material the most on sale, institute The method used is method well known to those skilled in the art.
Embodiment 1
Preparation 200g compositions, wherein Ramulus et Folium Mussaendae Pubescentis extract, the preparation method of Semen Vitis viniferae extract and compositions ratio Under such as:
(1) preparation of Ramulus et Folium Mussaendae Pubescentis extract: weigh 680g Ramulus et Folium Mussaendae Pubescentis and dry to constant weight in 50 DEG C of baking ovens, rear powder Broken, cross 80 mesh sieves, with the ethanol solution of volume fraction 50%, solid-liquid ratio is 1:20, extracts at 60 DEG C 2h, filters, takes filtrate, rotates evaporating ethanol, and carry out vacuum lyophilization and prepare Ramulus et Folium Mussaendae Pubescentis at 50 DEG C Extract 100g.
(2) preparation of Semen Vitis viniferae extract: weigh 200g Semen Vitis viniferae and dry to constant weight in 50 DEG C of baking ovens, Rear pulverizing, crosses 80 mesh sieves, and with the ethanol solution of volume fraction 50%, solid-liquid ratio is 1:20, carries at 60 DEG C Take 0.5h, filter, take filtrate, rotate evaporating ethanol at 50 DEG C, and it is prepared to carry out vacuum lyophilization Semen Vitis viniferae extract 20g.
Make the active component composition containing Ramulus et Folium Mussaendae Pubescentis extract, wherein Ramulus et Folium Mussaendae Pubescentis extract 100g, Cyclocarya paliurus Iljinskaja powder 20g, Powder of Folium Mori 20g, Fructus Crataegi powder 20g, Semen Vitis viniferae extract 20g, tea polyphenols 20g.
Embodiment 2
Preparation 200g compositions, wherein Ramulus et Folium Mussaendae Pubescentis extract, the preparation method of Semen Vitis viniferae extract and compositions ratio Under such as:
(1) preparation of Ramulus et Folium Mussaendae Pubescentis extract: weigh Ramulus et Folium Mussaendae Pubescentis 90g and dry to constant weight in 50 DEG C of baking ovens, pulverize afterwards, Crossing 80 mesh sieves, with the ethanol solution of volume fraction 80%, solid-liquid ratio is 1:10, extracts 3h at 30 DEG C, Filter, take filtrate, at 50 DEG C rotate evaporating ethanol, and carry out vacuum lyophilization prepare Ramulus et Folium Mussaendae Pubescentis extract Thing 120g.
(2) preparation of Semen Vitis viniferae extract: weigh Semen Vitis viniferae 308g and dry to constant weight in 50 DEG C of baking ovens, Rear pulverizing, crosses 80 mesh sieves, and with the ethanol solution of volume fraction 50%, solid-liquid ratio is 1:10, carries at 25 DEG C Take 2h, filter, take filtrate, rotate evaporating ethanol at 50 DEG C, and carry out vacuum lyophilization and prepare Portugal Grape seed extract 30g.
Make the active component composition containing Ramulus et Folium Mussaendae Pubescentis extract, wherein Ramulus et Folium Mussaendae Pubescentis extract 120g, Cyclocarya paliurus Iljinskaja powder 10g, Powder of Folium Mori 10g, Fructus Crataegi powder 10g, Semen Vitis viniferae extract 30g, tea polyphenols 20g.
Embodiment 3
The active component composition of embodiment 2 gained is conventionally prepared as pressed candy, when needing note Meaning, active component composition of the present invention can make any applicable dosage form as required, however it is not limited to Pressed candy, the present embodiment be made into pressed candy be stably be unlikely to deteriorate owing to pressed candy has character, And the advantage such as easy to carry.The pressed candy that the present embodiment is made is taken 3 times every day, each 2, permissible Realize daily health caring, the daily blood pressure stabilization maintaining hyperpietic etc. multiple efficacies.
Experimental example 1 oxidation resistance
Being divided into four groups, A group is Ramulus et Folium Mussaendae Pubescentis extract group, and B group is Semen Vitis viniferae extract group, and C group is institute of the present invention Stating Chinese medicinal effective-part composition group, D group is blank group.
Experimental study extract and the iron ion reducing power of compositions and DPPH radical scavenging activity, Its oxidation resistance is evaluated with this.Wherein sample ferrum assessment of reducibility index EC1 value, EC1 value refers to It is the amount (mmol of the antioxidant (sample) having identical oxidation resistance with 1mM FeSO4 7H2O Fe2+/g FW), light absorption value or EC1 value are the biggest, and its corresponding sample ferrum reducing power is the strongest, antioxidation Change activity the highest;Sample DPPH radical scavenging activity evaluation index is EC50 value, and EC50 value refers to By the concentration (g FW/L) of the antioxidant (sample) needed for the concentration reduction by 50% of original DPPH ethanol solution, Light absorption value or EC50 value are the least, and the ability removing DPPH free radical of its corresponding sample is the strongest, antioxygen Change activity the highest.Experimental result is as shown in table 1.
Table 1
Group EC1 value EC50 value
A group 437.1μmol/mL 0.281μg/mL
B group 382.2μmol/mL 0.321μg/mL
C group 896.3μmol/mL 0.102μg/mL
D group 0 0
As shown in Table 1, active component composition prepared by the present invention have certain iron ion reducing power and DPPH radical scavenging activity, and the iron ion reducing power of compositions and DPPH radical scavenging activity equal It is better than Ramulus et Folium Mussaendae Pubescentis extract and the Semen Vitis viniferae extract of one-component.Illustrate that active component composition of the present invention has There is certain oxidation resistance.
Experimental example 2 blood pressure lowering effect
Clinical observation patient is divided into 600 examples, and patient all has obvious hypertension clinical symptoms and through clinicist Make a definite diagnosis.
Patient is divided into 3 groups, and first group of pressed candy that embodiment 3 gained is administered orally, administration time is 20 days. Second group is administered orally pressed candy prepared by single Ramulus et Folium Mussaendae Pubescentis extract, three times a day, each 2.3rd group Pressed candy prepared by oral single Ampelopsis grossedentata leaf powder, three times a day, each 2.
After 15 days, the blood pressure data statistical result of patient is shown in Table 2.
Criterion of therapeutical effect: effective include two kinds of situations: the first is that diastolic pressure declines 1.3KPa (10mmHg) Above, and reach normal range;Though diastolic pressure does not drops to normally, but decline 2.6KPa (20mmHg) Above.The second is that diastolic pressure declines not as good as 1.3KPa (10mmHg), but has reached normal range;Relax Decline 1.3~2.34KPa (10~19mmHg) before opening pressure relatively treatment, but do not reach normal range;Shrink pressure relatively More than 4.0KP (30mmHg) is declined before treatment.Invalid: not up to effective standard.
Table 2
Group Number of cases Effective Invalid Obvious effective rate
1 200 98 2 98%
2 200 92 8 92%
3 200 85 15 85%
As shown in Table 2, active component composition of the present invention can significantly reduce the blood of hyperpietic Pressure, and follow up a case by regular visits to according to experiment is follow-up, the pressed candy that long-term taking active component composition of the present invention is made The hyperpietic of fruit, daily blood pressure is kept at normal level, it is not necessary to take depressor especially.

Claims (7)

1. the active component composition containing Ramulus et Folium Mussaendae Pubescentis extract, it is characterised in that described active component composition By forming according to the component of mass percentage as follows: Ramulus et Folium Mussaendae Pubescentis extract 50~60%, Cyclocarya paliurus Iljinskaja powder 5~10%, Powder of Folium Mori 5~10%, Fructus Crataegi powder 5~10%, Semen Vitis viniferae extract 10~15%, tea polyphenols 10~15%;
Wherein, the preparation method of described Ramulus et Folium Mussaendae Pubescentis extract is: dries Ramulus et Folium Mussaendae Pubescentis to constant weight, then pulverizes and sieves, Extract with ethanol solution, cross leaching filtrate, be dried after removing ethanol, obtain Ramulus et Folium Mussaendae Pubescentis extract;
The preparation method of described Semen Vitis viniferae extract is: dries Semen Vitis viniferae to constant weight, then pulverizes and sieves, Extract with ethanol solution, cross leaching filtrate, be dried after removing ethanol, obtain Semen Vitis viniferae extract;
The preparation method of described Cyclocarya paliurus Iljinskaja powder, powder of Folium Mori and Fructus Crataegi powder is: take blue or green money, Folium Mori, Fructus Crataegi respectively, Dry to constant weight, after pulverizing and sieving, respectively obtain Cyclocarya paliurus Iljinskaja powder, powder of Folium Mori and Fructus Crataegi powder.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract the most according to claim 1, it is characterised in that institute State active component composition by forming according to the component of mass percentage as follows: Ramulus et Folium Mussaendae Pubescentis extract 40~50%, Ampelopsis grossedentata leaf powder 10~20%, Cyclocarya paliurus Iljinskaja powder 5~10%, powder of Folium Mori 5~10%, Fructus Crataegi powder 5~10%, Semen Vitis viniferae extract 5~10%, tea polyphenols 5~10%;
Wherein, the preparation method of described Ampelopsis grossedentata leaf powder is: takes Ampelopsis grossedentata leaf and dries to constant weight, After pulverizing and sieving, obtain Ampelopsis grossedentata leaf powder.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract the most according to claim 1, it is characterised in that institute Stating in the preparation method of Ramulus et Folium Mussaendae Pubescentis extract, described drying is 50 DEG C of drying, described in sieve be 80 mesh sieves, institute The volume fraction stating ethanol solution is 50~80%, and the solid-liquid ratio of extraction is 1:10~1:20, and extraction temperature is 30~60 DEG C, extraction time is 2~3h, described is dried as vacuum lyophilization.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract the most according to claim 1, it is characterised in that institute Stating in the preparation method of Semen Vitis viniferae extract, described drying is 50 DEG C of drying, described in sieve be 80 mesh sieves, The volume fraction of described ethanol solution is 30~50%, and the solid-liquid ratio of extraction is 1:10~1:20, and extraction temperature is 25~60 DEG C, extraction time is 0.5~2h, described is dried as vacuum lyophilization.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract the most according to claim 1, it is characterised in that institute Stating in the preparation method of Cyclocarya paliurus Iljinskaja powder powder of Folium Mori and Fructus Crataegi powder, described drying is 50 DEG C of drying, described in sieve and be Cross 80 mesh sieves.
Active component composition containing Ramulus et Folium Mussaendae Pubescentis extract the most according to claim 2, it is characterised in that institute Stating in the preparation method of Ampelopsis grossedentata leaf powder, described drying is 50 DEG C of drying, described in sieve be 80 mesh sieves.
7. active component composition containing Ramulus et Folium Mussaendae Pubescentis extract described in claim 1~6 any one claim exists Preparation is for antioxidation, defying age, lowering blood pressure and blood fat, blood sugar lowering, radioprotective or antineoplastic health care Application in food or health care medicine.
CN201610428974.9A 2016-06-16 2016-06-16 Effective part composition containing white tea extracts and preparation method and application of effective part composition Pending CN105851352A (en)

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CN112167406A (en) * 2020-11-09 2021-01-05 浙江一鸣食品股份有限公司 Preparation method of black tea fermented tea beverage

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Application publication date: 20160817