CN105851076A - Biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests - Google Patents
Biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests Download PDFInfo
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- CN105851076A CN105851076A CN201610276094.4A CN201610276094A CN105851076A CN 105851076 A CN105851076 A CN 105851076A CN 201610276094 A CN201610276094 A CN 201610276094A CN 105851076 A CN105851076 A CN 105851076A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/88—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms six-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests and a preparation method of the biopesticide. The biopesticide is prepared from, by mass, 15-24 parts of buprofezin, 3-5 parts of Bacillus sphaericus, 2.5-3.5 parts of purple bergenia herb extracts, 1.5-2.5 parts of cortex meliae powder, 2.3-3.6 parts of potassium nitrate, 5-6 parts of dispersing agent, 1.5-2.6 parts of wetting agent, 2.6-3.6 parts of diffusant, 7-13 parts of kieselguhr and 13-21 parts of water. The prepared biopesticide has a good prevention and treatment effect in soybean plantation fields 15 days after being applied, and the prevention effects on frequently-seen injurious pests including soybean pod borers, red spider mites, clanis bilineata walkers, bean borers and Ascotis selenaria Schiffermuller et Denis in soybean plantation fields reach 96.1-99.8%, 88.7-91.5%, 94.4-98.6%, 83.2-86.9% and 73.8-78.9% respectively.
Description
Technical field
The present invention relates to a kind of containing Bacillus sphaericus for preventing and treating biological insecticides and the preparation thereof of soybean diseases and insect pests
Method, belongs to technical field of pesticide.
Background technology
Bacillus sphaericus be a kind of widely distributed in nature, form sub-end and expand sporangium and spherical gemma
Aerobic bacillus.The toxin protein that the toxic action of different insects is mainly generated by by Bacillus sphaericus realizes.
Having been demonstrated that and can produce two class difference toxin proteins in its growth and development process, a class is present in all supper toxic strains
Crystal toxin albumen；The another kind of Mtx toxin protein being present in Hypovirulent strain in part supper toxic strain.
Crops are all had a certain degree of Side effect by existing chemical insecticide, can cause soil in various degree
The environmental pollutions such as ground, water source, chemical insecticide improper use can cause crops to produce poisoning, causes crop growth abnormal,
Suppress its photosynthesis, affect crop yield, and second stubble crop be will also result in certain murder by poisoning.
Existing chemical insecticide, is divided into liquid and solid pharmaceutical preparation two types, liquid preparation dispersible oil-suspending agent,
Missible oil, aqueous emulsion, microemulsion；Solid pharmaceutical preparation has wettable powder, soluble powder, water dispersible granules.
The defect that existing chemical insecticide exists is as follows:
(1) existing insecticide, just for the insect pest in planting site, not notable or the most anti-to the diseases control in planting site
Effect, and existing insecticide is the most obvious to certain insect insecticidal effect.
(2) when existing insecticide is applied to soybean pest control, having certain side effect to soybean, poor stability, to it
Growth has certain inhibitory action, and then reduces crop yield.
(3) after existing insecticide has been used, easily causing insecticide toxin residual, the quality of soybean produces impact,
Affect the edible safety of soybean.
Summary of the invention
The present invention solves the deficiency that prior art exists, it is provided that a kind of Bacillus sphaericus that contains is used for preventing and treating soybean disease
Biological insecticides of insect pest and preparation method thereof, to realize following goal of the invention:
(1) insecticide of the present invention is effective to the pest control of salt-soda soil soybean, eating-core bean worm, red spider, greenish brown hawk moth, beans
Olethreutid, the preventive effect of big bridging worm significantly improve；
(2) insecticide prepared by the present invention is simultaneously good to the disease-controlling effect of salt-soda soil soybean, to common in soybean planting ground
Disease: downy mildew, grey speck of soybean, soybean sclerotinia crown rot, soybean bacterial spot are sick, the anti-imitation of shy disease of withering of soybean is aobvious
Write；
(3), during insecticide prepared by the present invention is applied to salt-soda soil soybean during pest control, soybean is had no side effect, security
Height, Bacillus sphaericus after death can decompose at host insects, will not produce insecticide toxin residual.
For solving above technical problem, the technical solution used in the present invention is as follows:
A kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests, described insecticide, including Buprofezin,
Bacillus sphaericus, purple bergenia herb extract, azedarach powder, potassium nitrate.
The following is and the further of technique scheme is improved:
Described insecticide, the mass parts content of each raw material components is Buprofezin 24-15 part, Bacillus sphaericus 3-5 part, purple bergenia herb
Extract 2.5-3.5 part, azedarach powder 1.5-2.5 part, potassium nitrate 2.3-3.6 part, dispersant 5-6 part, wetting agent 1.5-2.6
Part, diffusant 2.6-3.6 part, diatomite 7-13 part, water 13-21 part.
Described wetting agent is sodium butylnaphthalenesulfonate salt；Described dispersant is methyl naphthalene sulfonic acid salt formaldehyde condensation products；Described expansion
Powder is naphthalenesulfonate formaldehyde condensation compound.
Described diatomite contains silica, iron oxide, aluminum oxide, and silica content is 89%, and iron oxide content is 1.3%,
Alumina content is 6%.
Described azedarach powder contains toosendanin, and the weight/mass percentage composition of toosendanin active ingredient is 2.8%.
Described purple bergenia herb extract contains Bergenin, and the content of Bergenin is 98%.
A kind of containing Bacillus sphaericus for the biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof, described system
Preparation Method includes that the preparation of purple bergenia herb extract, the preparation of described purple bergenia herb extract include pulverizing extraction step, concentration step
And absorb-elute step.
Described pulverizing extraction step, takes purple bergenia herb and cleans decontamination, and by purple bergenia herb grind into fine powder, it is white that addition is equivalent to rock
10 times of saturated limewaters of dish powder, regulation pH value is 11, extracts three times continuously, and each extraction time is 4.5h, merges three times carry
Take liquid.
Described concentration step, will pulverize extraction step prepared extract Rotary Evaporators decompression and distill, concentrated filtrate,
Extract normal pressure is concentrated into the 1.15-1.20 of its proportion, and to add concentrated hydrochloric acid regulation pH value be 3, stir, at 65-75
DEG C heating 10min, ambient temperatare puts 5.5-7h, the CE to purple bergenia herb extract of filtration.
A kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests and at salt-soda soil Soybean Species
Application in planting, the consumption of described insecticide is 25-30g/ mu.
Use above technical scheme, the invention have the benefit that
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in soybean planting ground, to common in soybean planting ground
Insect: eating-core bean worm, red spider, greenish brown hawk moth, beans olethreutid, the preventive effect of big bridging worm respectively reach 96.1-99.8%,
88.7-91.5%、94.4-98.6%、83.2-86.9%、73.8-78.9%。
(2) while insecticide prepared by the present invention insect in carrying out soybean planting ground, the disease to soybean plant strain
Preventing and treating has effect equally.Disease to common in soybean planting ground: downy mildew, grey speck of soybean, soybean sclerotinia crown rot, big
Beans bacterial spot is sick, the preventive effect of the shy disease of withering of soybean respectively reaches 86.5-90.5%, 84.5-88.6%, 74.3-79.5%, 75.9-
80.6%、88.6-92.2%。
(3) insecticide that prepared by the present invention is without any side effects to soybean plant strain, and security is high, also obtains beyond thought
Effect, the effect of being significantly improved of the yield of soybean plant strain, per mu yield reaches 340-380 jin；In soybean, protein and fat mass also show
Writing and improve, protein content is 33-37g/100g, and fat content is 17-21g/100g.
(4) Bacillus sphaericus after death can decompose at host insects, will not produce insecticide toxin residual.
Detailed description of the invention
Hereinafter the preferred embodiments of the present invention are illustrated, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Bacillus sphaericus
Described insecticide, the mass ratio of each raw material components is:
Buprofezin 15 parts, Bacillus sphaericus 3 parts, purple bergenia herb extract 2.5 parts, 1.5 parts of azedarach powder, potassium nitrate 2.3 part, point
Powder 5 parts, wetting agent 1.5 parts, diffusant 2.6 parts, 7 parts of diatomite, 13 parts of water.
Described Buprofezin, the weight/mass percentage composition of active ingredient is 25%；
Described Bacillus sphaericus, the mass content of active ingredient be 40,000,000,000 spores/gram
Described purple bergenia herb extract contains Bergenin, and the content of Bergenin is 98%；
Described azedarach powder contains toosendanin, and the weight/mass percentage composition of toosendanin active ingredient is 2.8%；
Described potassium nitrate, the weight/mass percentage composition of active ingredient is 99%；
Described wetting agent is sodium butylnaphthalenesulfonate salt；
Described dispersant is methyl naphthalene sulfonic acid salt formaldehyde condensation products；
Described diffusant is naphthalenesulfonate formaldehyde condensation compound；
Described diatomite contains silica, iron oxide, aluminum oxide, and silica content is 89%, and iron oxide content is 1.3%, oxidation
Aluminium content is 6%.
Embodiment 2 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Bacillus sphaericus
Described insecticide, the mass ratio of each raw material components is:
Buprofezin 20 parts, Bacillus sphaericus 4 parts, purple bergenia herb extract 3 parts, 2 parts of azedarach powder, 3 parts of potassium nitrate, dispersant 5
Part, wetting agent 2 parts, diffusant 3 parts, 10 parts of diatomite, 17 parts of water.
Described Buprofezin, the weight/mass percentage composition of active ingredient is 25%；
Described Bacillus sphaericus, the mass content of active ingredient be 40,000,000,000 spores/gram
Described purple bergenia herb extract contains Bergenin, and the content of Bergenin is 98%；
Described azedarach powder contains toosendanin, and the weight/mass percentage composition of toosendanin active ingredient is 2.8%；
Described potassium nitrate, the weight/mass percentage composition of active ingredient is 99%；
Described wetting agent is sodium butylnaphthalenesulfonate salt；
Described dispersant is methyl naphthalene sulfonic acid salt formaldehyde condensation products；
Described diffusant is naphthalenesulfonate formaldehyde condensation compound；
Described diatomite contains silica, iron oxide, aluminum oxide, and silica content is 89%, and iron oxide content is 1.3%, oxidation
Aluminium content is 6%.
Embodiment 3 one kinds is used for preventing and treating the biological insecticides of soybean diseases and insect pests containing Bacillus sphaericus
Described insecticide, the mass ratio of each raw material components is:
Buprofezin 24 parts, Bacillus sphaericus 5 parts, purple bergenia herb extract 3.5 parts, 2.5 parts of azedarach powder, potassium nitrate 3.6 part, point
Powder 6 parts, wetting agent 2.6 parts, diffusant 3.6 parts, 13 parts of diatomite, 21 parts of water.
Described Buprofezin, the weight/mass percentage composition of active ingredient is 25%；
Described Bacillus sphaericus, the mass content of active ingredient be 40,000,000,000 spores/gram
Described purple bergenia herb extract contains Bergenin, and the content of Bergenin is 98%；
Described azedarach powder contains toosendanin, and the weight/mass percentage composition of toosendanin active ingredient is 2.8%；
Described potassium nitrate, the weight/mass percentage composition of active ingredient is 99%；
Described wetting agent is sodium butylnaphthalenesulfonate salt；
Described dispersant is methyl naphthalene sulfonic acid salt formaldehyde condensation products；
Described diffusant is naphthalenesulfonate formaldehyde condensation compound；
Described diatomite contains silica, iron oxide, aluminum oxide, and silica content is 89%, and iron oxide content is 1.3%, oxidation
Aluminium content is 6%.
Bacillus sphaericus used by above example 1 to embodiment 3 is buied by existing product on market.
Embodiment 4 one kinds is used for biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof containing Bacillus sphaericus
(1) preparation of purple bergenia herb extract:
A, pulverize, extract
Take purple bergenia herb and clean decontamination, by purple bergenia herb grind into fine powder, add and be equivalent to 10 times of saturated limewaters of purple bergenia herb powder, adjust
Joint pH value is 11, extracts three times continuously, and each extraction time is 4.5h, merges the extract of three times；
B, concentration
By the Rotary Evaporators decompression distillation of said extracted liquid, concentrated filtrate, extract normal pressure is concentrated into the 1.15-of its proportion
1.20, and add concentrated hydrochloric acid regulation pH value be 3, stir, 65-75 DEG C heat 10min, ambient temperatare puts 5.5-7h, mistake
The CE to purple bergenia herb extract of filter；
C, adsorb, elute
By above-mentioned crude extract through macroreticular resin (D101) adsorption bleaching, after 95% ethanol elution, eluent is concentrated and is evaporated, low temperature
Freeze-drying, obtains purple bergenia herb extract；
(2) weigh
According to the insecticidal formulation described in embodiment 1-3, weigh each composition.
(3) mixing
By Buprofezin, Bacillus sphaericus, purple bergenia herb extract, azedarach powder, potassium nitrate, dispersant, wetting agent, diffusant and
Diatomite puts in blender and fully mixes；
(4) pulverize
Solid material after above-mentioned mixing is put in airslide disintegrating mill, is crushed to suitable fineness through airslide disintegrating mill and divides
Cloth；
(5) mediate
Putting in kneader by above-mentioned crushing rear material, uniformly add water in kneader kneading；
(6) granulation, be dried
Above-mentioned kneaded wet stock is put into extruding pelletization in comminutor, the wet granular of extrusion is gone in fluid bed,
Being dried under the airflow function of proper temperature, in product, residual moisture content controls to be advisable in 0.5～1.0%, prepares insecticide.
Embodiment 5 one kinds is used for the biological insecticides preventing and treating soybean diseases and insect pests on soybean planting ground containing Bacillus sphaericus
Middle application
Four experimental plots, respectively experimental plot A, experimental plot B, experimental plot C and examination is hewed out in soybean planting ground
Testing community D, the length of each experimental plot is about 10-15m, and the consumption of insecticide is 25-30g/ mu.
Experimental plot A: product in embodiment 1 is watered dilution by 700 times, uniform to the soybean plant strain in the A of experimental plot
Spraying, in soybean seedling stage, go out a phase, florescence and fruiting period and respectively spray 2 medicines, spray date is separated by 6-9 days, is sprayed onto blade and opens
Begin drippage for degree.
Experimental plot B: product in embodiment 2 is watered dilution by 700 times, uniform to the soybean plant strain in the B of experimental plot
Spraying, in soybean seedling stage, go out a phase, florescence and fruiting period and respectively spray 2 medicines, spray date is separated by 6-9 days, is sprayed onto blade and opens
Begin drippage for degree.
Experimental plot C: product in embodiment 3 is watered dilution by 700 times, uniform to the soybean plant strain in the C of experimental plot
Spraying, in soybean seedling stage, go out a phase, florescence and fruiting period and respectively spray 2 medicines, spray date is separated by 6-9 days, is sprayed onto blade and opens
Begin drippage for degree.
Experimental plot D: as check experiment community, does not spray this insecticide, replaces insecticide to experimental plot D with clear water
In soybean plant strain even spraying.
Investigation and statistical method: investigation in 15 days after fruiting period dispenser, each experimental plot is investigated 100 strain soybean, is added up
Killed strain number, checks prevention effect.
Result of the test:
(1) insecticide prepared by the present invention is good to the prevention effect of the insect in soybean planting ground, to common in soybean planting ground
Insect: eating-core bean worm, red spider, greenish brown hawk moth, beans olethreutid, the preventive effect of big bridging worm respectively reach 96.1-99.8%,
88.7-91.5%, 94.4-98.6%, 83.2-86.9%, 73.8-78.9%, specific targets are shown in Table 1.
Insecticide prepared by table 1 present invention prevention effect to the insect in soybean planting ground
(2) while insecticide prepared by the present invention insect in carrying out soybean planting ground, the disease control to soybean plant strain
There is effect equally.
Disease to common in soybean planting ground: downy mildew, grey speck of soybean, soybean sclerotinia crown rot, soybean bacterial plaque
Point is sick, the preventive effect of the shy disease of withering of soybean respectively reaches 86.5-90.5%, 84.5-88.6%, 74.3-79.5%, 75.9-80.6%,
88.6-92.2%, specific targets are shown in Table 2.
Insecticide prepared by table 2 present invention prevention effect to the disease in soybean planting ground
(3) insecticide that prepared by the present invention is without any side effects to soybean plant strain, and security is high, also obtains beyond thought effect
Really, the effect of being significantly improved of the yield of soybean plant strain, per mu yield reaches 340-380 jin；In soybean, protein and fat mass are the most notable
Improving, protein content is 33-37g/100g, and fat content is 17-21g/100g, is specifically shown in Table 3.
Protein and dietary fiber content in the yield of table 3 soybean, soybean
Comparison: do not use insecticide of the present invention, replaces insecticide to spray with clear water.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent.
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (10)
1. one kind contains Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests, it is characterised in that: described desinsection
Agent, including Buprofezin, Bacillus sphaericus, purple bergenia herb extract, azedarach powder, potassium nitrate.
The most according to claim 1 a kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests,
It is characterized in that: described insecticide, the mass parts content of each raw material components is Buprofezin 24-15 part, Bacillus sphaericus 3-5
Part, purple bergenia herb extract 2.5-3.5 part, azedarach powder 1.5-2.5 part, potassium nitrate 2.3-3.6 part, dispersant 5-6 part, wetting agent
1.5-2.6 part, diffusant 2.6-3.6 part, diatomite 7-13 part, water 13-21 part.
The most according to claim 2 a kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests,
It is characterized in that: described wetting agent is sodium butylnaphthalenesulfonate salt；Described dispersant is methyl naphthalene sulfonic acid salt formaldehyde condensation products；Described
Diffusant is naphthalenesulfonate formaldehyde condensation compound.
The most according to claim 2 a kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests,
It is characterized in that: described diatomite contains silica, iron oxide, aluminum oxide, and silica content is 89%, and iron oxide content is
1.3%, alumina content is 6%.
The most according to claim 2 a kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests,
It is characterized in that: described azedarach powder contains toosendanin, the weight/mass percentage composition of toosendanin active ingredient is 2.8%.
The most according to claim 2 a kind of containing Bacillus sphaericus for preventing and treating the biological insecticides of soybean diseases and insect pests,
It is characterized in that: described purple bergenia herb extract contains Bergenin, the content of Bergenin is 98%.
7. one kind is used for biological insecticides preventing and treating soybean diseases and insect pests and preparation method thereof containing Bacillus sphaericus, and its feature exists
In: described preparation method include the preparation of purple bergenia herb extract, the preparation of described purple bergenia herb extract include pulverizing extraction step,
Concentration step and absorb-elute step.
The most according to claim 7 a kind of containing Bacillus sphaericus for prevent and treat soybean diseases and insect pests biological insecticides and
Its preparation method, it is characterised in that: described pulverizing extraction step, take purple bergenia herb and clean decontamination, by purple bergenia herb grind into fine powder,
Adding and be equivalent to 10 times of saturated limewaters of purple bergenia herb powder, regulation pH value is 11, extracts three times continuously, and each extraction time is
4.5h, merges the extract of three times.
The most according to claim 7 a kind of containing Bacillus sphaericus for prevent and treat soybean diseases and insect pests biological insecticides and
Its preparation method, it is characterised in that: described concentration step, reduces pressure the extract Rotary Evaporators pulverizing extraction step prepared
Distillation, concentrated filtrate, extract normal pressure is concentrated into the 1.15-1.20 of its proportion, and to add concentrated hydrochloric acid regulation pH value be 3, stir
Mixing uniformly, heat 10min at 65-75 DEG C, ambient temperatare puts 5.5-7h, the CE to purple bergenia herb extract of filtration.
10. one kind is used for preventing and treating the biological insecticides of soybean diseases and insect pests and at salt-soda soil soybean planting containing Bacillus sphaericus
In application, it is characterised in that: the consumption of described insecticide is 25-30g/ mu.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610276094.4A CN105851076A (en) | 2016-04-29 | 2016-04-29 | Biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610276094.4A CN105851076A (en) | 2016-04-29 | 2016-04-29 | Biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests |
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| CN105851076A true CN105851076A (en) | 2016-08-17 |
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| CN201610276094.4A Pending CN105851076A (en) | 2016-04-29 | 2016-04-29 | Biopesticide containing Bacillus sphaericus and used for preventing and treating soybean diseases and pests |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713516A (en) * | 2019-03-20 | 2020-09-29 | 安徽省农业科学院蚕桑研究所 | Composition for preventing and treating longicorn and prevention and treatment method |
| CN115737628A (en) * | 2022-02-23 | 2023-03-07 | 安阳工学院 | Application of bergenin sulfonate derivative in resisting activity of drug-resistant bacteria |
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| CN101962385A (en) * | 2010-10-19 | 2011-02-02 | 南京泽朗医药科技有限公司 | Method for preparing bergeninum |
| CN103262865A (en) * | 2013-06-01 | 2013-08-28 | 管天球 | Preparation method of tea shell mosquito-repellent incense |
| CN103444707A (en) * | 2013-08-01 | 2013-12-18 | 广东中迅农科股份有限公司 | Environment-friendly type wettable powder capable of being directly applied to rice field |
| CN104336093A (en) * | 2013-07-31 | 2015-02-11 | 青岛艾华隆生物科技有限公司 | Microbial pesticide for rice |
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| CN101962385A (en) * | 2010-10-19 | 2011-02-02 | 南京泽朗医药科技有限公司 | Method for preparing bergeninum |
| CN103262865A (en) * | 2013-06-01 | 2013-08-28 | 管天球 | Preparation method of tea shell mosquito-repellent incense |
| CN104336093A (en) * | 2013-07-31 | 2015-02-11 | 青岛艾华隆生物科技有限公司 | Microbial pesticide for rice |
| CN103444707A (en) * | 2013-08-01 | 2013-12-18 | 广东中迅农科股份有限公司 | Environment-friendly type wettable powder capable of being directly applied to rice field |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713516A (en) * | 2019-03-20 | 2020-09-29 | 安徽省农业科学院蚕桑研究所 | Composition for preventing and treating longicorn and prevention and treatment method |
| CN115737628A (en) * | 2022-02-23 | 2023-03-07 | 安阳工学院 | Application of bergenin sulfonate derivative in resisting activity of drug-resistant bacteria |
| CN115737628B (en) * | 2022-02-23 | 2024-04-12 | 安阳工学院 | Application of bergenin sulfonate derivative in resisting drug-resistant bacteria activity |
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