CN105850986B - A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe - Google Patents
A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe Download PDFInfo
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- CN105850986B CN105850986B CN201610396509.1A CN201610396509A CN105850986B CN 105850986 B CN105850986 B CN 105850986B CN 201610396509 A CN201610396509 A CN 201610396509A CN 105850986 B CN105850986 B CN 105850986B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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Abstract
The present invention relates to a kind of people's rareness sperm DPV refrigeration systems of highly effective and safe, including safe droplet slide glass, freezing droplet and liquid nitrogen vapor tank, the safe droplet slide glass is made of biological safety sterile material, safe droplet slide glass is divided into several regions of the same area, and safe droplet slide glass is that unified material is integrally formed；The freezing droplet is made of the special cryoprotection drop of fixed capacity；Bottom is equipped with liquid nitrogen in the liquid nitrogen vapor tank, and liquid nitrogen vapor is full of in tank, and safe droplet slide glass is placed in freezing housing and is placed in liquid nitrogen vapor tank.The invention further relates to a kind of people's rareness sperm DPV freezing methods.Advantages of the present invention is embodied in: using the biological safety sterile material of optimum thickness, convenient for efficiently utilizing the frozen sperm on the safe droplet slide glass；Safe droplet slide glass is that unified material is integrally formed, and prevents other materials from polluting；With region division, more preferably it is convenient for long-term preservation, more saving in liquid nitrogen vapor prevents cross contamination.
Description
Technical field
The present invention relates to assisted reproduction fields, and in particular to a kind of people's rareness sperm DPV refrigeration system of highly effective and safe and
Freezing method.
Background technique
The infertility of male factor has accounted for 40-50% at present, and there are also more serious development trends.It is more next
The disease incidence of higher few azoospermia seriously affects the fecundity of male.Although we can be by testicular biopsy or micro-
Cut obtain sperm, but at present lack highly effective and safe freezen protective rareness sperm, especially single sperm freezing carrier and
Method.
Chinese patent (CN 104823966A) discloses a kind of micro sperm cryopreservation carrier and corresponding freezing reconciliation
Jelly method, but its carrier medium is to lack safety and lack region division and can not effectively prevent intersecting dirt between sample
Dye.
Summary of the invention
It is rare the purpose of the present invention is aiming at the shortcomings in the prior art, providing a kind of people of highly effective and safe no cross contamination
Sperm (contains monosperm, as testicular biopsy sperm, epididymis puncture the micro- incision essence of sperm, Azoospermia patient sperm, testis
Son etc.) DPV refrigeration system.
To achieve the above object, the invention discloses following technical solutions:
A kind of people's rareness sperm DPV refrigeration system of highly effective and safe, including safe droplet slide glass, freezing droplet and liquid nitrogen steam
Vapour tank, the safe droplet slide glass are made of biological safety sterile material, and safe droplet slide glass is divided into several identical
The region of area, safe droplet slide glass are that unified material is integrally formed；The freezing droplet is protected by the special freezing of fixed capacity
It protects drop to constitute, freezing droplet is dripped when in use on safe droplet slide glass；Bottom is equipped with liquid nitrogen in the liquid nitrogen vapor tank, and
And liquid nitrogen vapor is full of in tank, safe droplet slide glass is placed in freezing housing and is placed in liquid nitrogen vapor tank.
Further, the safe droplet slide glass is rectangular tab.
Further, the material of the safe droplet slide glass is thin using the biological safety polypropylene of ethylene oxide sterilizing
Piece.
Further, the safe droplet slide glass with a thickness of 1.5 μm.
Further, the safe droplet slide glass is divided into 8 regions of the same area.
Further, the safe droplet slide glass is equipped with the handle of an identical material, handle and safe droplet slide glass one
It is body formed.
Further, height of the liquid nitrogen liquid level that the liquid nitrogen vapor pot bottom is equipped with not higher than freezing housing.
Further, bracket is equipped in the liquid nitrogen vapor tank, safe droplet slide glass is placed in freezing housing and passes through
Bracket is placed in liquid nitrogen vapor tank.
Another object of the present invention is to provide a kind of people's rareness sperm of highly effective and safe (containing monosperm, such as testicular biopsy essence
Son, epididymis puncture sperm, Azoospermia patient sperm, micro- incision sperm of testis etc.) freezing and storing method, utilization is above-mentioned
DPV refrigeration system, specifically comprise the following steps:
The micro sperm freezing housing of S1: drop has freezing droplet on safe droplet slide glass, is added in each freezing droplet corresponding
Sperm after, by the safe droplet slide glass be put into in the matched freezing housing of its size with other sperm samples isolation open, so
The freezing housing for being loaded with safe droplet slide glass liquid nitrogen is carried out afterwards to fumigate；
The micro sperm long-term preservation of S2: prepare the liquid nitrogen vapor tank that a bottom is equipped with liquid nitrogen, and keep liquid nitrogen vapor
Liquid nitrogen vapor is full of in tank, the freezing housing for being loaded with safe droplet slide glass is rack-mount, it is placed on liquid nitrogen steaming for a long time
It is saved in vapour.
The people's rareness sperm DPV refrigeration system and freezing method of a kind of highly effective and safe disclosed by the invention have with following
Beneficial effect:
Safe droplet slide glass uses the biological safety sterile material of optimum thickness, convenient for efficiently being carried using the safe droplet
The frozen sperm of on piece；
Safe droplet slide glass is that unified material is integrally formed, and can prevent other materials from polluting；
With region division, more preferably it is convenient for long-term preservation, and cross contamination between sample can be prevented.
Detailed description of the invention
Fig. 1 is the combination diagram of safe droplet slide glass and freezing housing；
Fig. 2 is the step schematic diagram (1) that sperm sample is packed into freezing droplet；
Fig. 3 is the step schematic diagram (2) that sperm sample is packed into freezing droplet
Fig. 4 is the step schematic diagram of micro sperm long-term preservation；
Fig. 5 is the step schematic diagram for taking out frozen sperm.
Description of symbols:
1. housing is freezed, 2. handles, 3. safe droplet slide glasses, 4. sperm samples, 5. freezing droplets, 6. brackets, 7. liquid nitrogen
Vapor can, 8. liquid nitrogen.
Specific embodiment
Below with reference to embodiment and referring to attached drawing, the invention will be further described.
- Fig. 4 referring to Figure 1.
A kind of people's rareness sperm DPV refrigeration system of highly effective and safe, including safe droplet slide glass 1, freezing droplet 5 and liquid nitrogen
Vapor can 7, the safe droplet slide glass 3 are made of biological safety sterile material, and safe droplet slide glass 3 is divided into several
Region of the same area, convenient for efficiently utilizing the frozen sperm on the freezing carrier, safe droplet slide glass 3 is unified material one
Molding, prevents other materials from polluting；The freezing droplet 5 is made of the special cryoprotection drop of fixed capacity, freezes droplet 5
It is dripped when in use on safe droplet slide glass 3；Bottom is equipped with liquid nitrogen 8 in the liquid nitrogen vapor tank 7, and liquid nitrogen is full of in tank
Steam, safe droplet slide glass 3 are placed in freezing housing 1 and are placed in liquid nitrogen vapor tank 7.
As specific embodiment, the safe droplet slide glass 3 is Thin Rectangular slide glass.
As specific embodiment, the material of the safe droplet slide glass 3 is poly- using the biological safety of ethylene oxide sterilizing
Propylene thin slice, the carrier are the biological safety sterile material of optimum thickness.
As specific embodiment, the safe droplet slide glass 3 with a thickness of 1.5 μm.
As specific embodiment, the safe droplet slide glass 3 is divided into 8 regions of the same area.
As specific embodiment, the safe droplet slide glass 3 is equipped with the handle 2 of an identical material, and handle 2 and safety are micro-
Slide glass 3 is dripped to be integrally formed.
As specific embodiment, 8 liquid level of liquid nitrogen that 7 bottom of liquid nitrogen vapor tank is equipped with is not higher than freezing housing 1
Height.
See Fig. 4, as specific embodiment, is equipped with bracket 6 in the liquid nitrogen vapor tank 7, safe droplet slide glass 3 is placed on cold
Freeze in housing 1 and is placed in liquid nitrogen vapor tank 7 by bracket 6.
A kind of people's rareness sperm cryopreservation method of highly effective and safe disclosed by the invention, using above-mentioned DPV refrigeration system,
Include the following steps:
The micro sperm freezing housing of step 1: drop has freezing droplet 5 on safe droplet slide glass 3, adds in each freezing droplet 5
After entering corresponding sperm, by the safe droplet slide glass 3 be put into in the matched freezing housing 1 of its size with other sperm samples every
It opens absolutely, the freezing housing 1 for being loaded with safe droplet slide glass 3 is then carried out liquid nitrogen and is fumigated.
The micro sperm long-term preservation of step 2: prepare the liquid nitrogen vapor tank 7 that a bottom is equipped with liquid nitrogen 8, and keep liquid nitrogen
It is full of liquid nitrogen vapor in vapor can 7, on the frame 6 by the installation of freezing housing 1 for being loaded with safe droplet slide glass 3, places for a long time
It is saved in liquid nitrogen vapor.
See Fig. 5, when taking out frozen sperm sample, takes out the freezing housing 1 for having safe droplet slide glass 3 first, will pacify
Full droplet slide glass 3 is placed in sampling ware, and sampled instrument draws frozen sperm sample.
The present invention uses the biological safety sterile material of optimum thickness, convenient for efficiently utilizing on the safe droplet slide glass
Frozen sperm；Safe droplet slide glass is that unified material is integrally formed, and can prevent other materials from polluting；With region division, more preferably
Convenient for long-term preservation.
The above is only a preferred embodiment of the present invention, rather than its limitations；Although should be pointed out that referring to above-mentioned each
Embodiment describes the invention in detail, those skilled in the art should understand that, it still can be to above-mentioned each
Technical solution documented by embodiment is modified, or equivalent substitution of some or all of the technical features；And this
A little modifications and replacement, do not make the essence of corresponding technical solution depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (4)
1. a kind of people's rareness sperm DPV refrigeration system of highly effective and safe, which is characterized in that micro- including safe droplet slide glass, freezing
Drop and liquid nitrogen vapor tank, the safe droplet slide glass are made of biological safety sterile material, and safe droplet slide glass is divided
For 8 regions of the same area, safe droplet slide glass is that unified material is integrally formed；The droplet that freezes is by the spy of fixed capacity
Different cryoprotection drop is constituted, and freezing droplet is dripped when in use on safe droplet slide glass；Bottom fills in the liquid nitrogen vapor tank
There is liquid nitrogen, and be full of liquid nitrogen vapor in tank, safe droplet slide glass is placed in freezing housing and is placed on liquid nitrogen vapor tank
It is interior；The safe droplet slide glass is rectangular tab, and the material of safe droplet slide glass uses the biological safety of ethylene oxide sterilizing
Polypropylene sheet, safe droplet slide glass with a thickness of 1.5 μm；Bracket is equipped in the liquid nitrogen vapor tank, safe droplet slide glass is put
It sets in freezing housing and is placed in liquid nitrogen vapor tank by bracket.
2. a kind of people's rareness sperm DPV refrigeration system of highly effective and safe according to claim 1, which is characterized in that described
Safe droplet slide glass is equipped with the handle of an identical material, and handle and safe droplet slide glass are integrally formed.
3. a kind of people's rareness sperm DPV refrigeration system of highly effective and safe according to claim 1, which is characterized in that described
Height of the liquid nitrogen liquid level that liquid nitrogen vapor pot bottom is equipped with not higher than freezing housing.
4. a kind of people's rareness sperm cryopreservation method of highly effective and safe, which is characterized in that using as described in claim 1-3
DPV refrigeration system, includes the following steps:
The micro sperm freezing housing of S1: drop has freezing droplet on safe droplet slide glass, and corresponding essence is added in each freezing droplet
After son, by the safe droplet slide glass be put into in the matched freezing housing of its size with other sperm samples isolation open, then
The freezing housing for being loaded with safe droplet slide glass carries out liquid nitrogen and fumigates；
The micro sperm long-term preservation of S2: prepare the liquid nitrogen vapor tank that a bottom is equipped with liquid nitrogen, and keep in liquid nitrogen vapor tank
It is full of liquid nitrogen vapor, the freezing housing for being loaded with safe droplet slide glass is rack-mount, it is placed in liquid nitrogen vapor for a long time
It is saved.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610396509.1A CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610396509.1A CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
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| Publication Number | Publication Date |
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| CN105850986A CN105850986A (en) | 2016-08-17 |
| CN105850986B true CN105850986B (en) | 2019-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610396509.1A Active CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220053756A1 (en) * | 2019-01-08 | 2022-02-24 | Changzhou Maternal And Child Health Care Hospital | Chip carrier for single sperm or extremely small amount of sperms |
| CN113040132B (en) * | 2021-03-22 | 2022-03-25 | 浙江大学 | Sperm cryopreservation method based on protective agent |
| CN113712025A (en) * | 2021-09-14 | 2021-11-30 | 荆州市中心医院 | Cryopreservation method of trace sperms and preparation method of sperms directly used for ICSI |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671651A (en) * | 2008-09-09 | 2010-03-17 | 上海交通大学医学院附属第九人民医院 | Sperm freezing and unfreezing method and sperm freezing and unfreezing device |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN204888506U (en) * | 2015-07-22 | 2015-12-23 | 柳州市妇幼保健院 | Efficient biomaterial freezes and deposits carrier device |
-
2016
- 2016-06-06 CN CN201610396509.1A patent/CN105850986B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671651A (en) * | 2008-09-09 | 2010-03-17 | 上海交通大学医学院附属第九人民医院 | Sperm freezing and unfreezing method and sperm freezing and unfreezing device |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN204888506U (en) * | 2015-07-22 | 2015-12-23 | 柳州市妇幼保健院 | Efficient biomaterial freezes and deposits carrier device |
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| CN105850986A (en) | 2016-08-17 |
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