CN206182196U - Rare sperm DPV refrigeration system of high -efficient safe people - Google Patents
Rare sperm DPV refrigeration system of high -efficient safe people Download PDFInfo
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- CN206182196U CN206182196U CN201620544372.5U CN201620544372U CN206182196U CN 206182196 U CN206182196 U CN 206182196U CN 201620544372 U CN201620544372 U CN 201620544372U CN 206182196 U CN206182196 U CN 206182196U
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- safe
- slide glass
- liquid nitrogen
- freezing
- droplet
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Abstract
The utility model relates to a rare sperm DPV refrigeration system of high -efficient safe people, including freezing overcoat, safe droplet slide glass and liquid nitrogen steam drum, safe droplet slide glass adopts bio -safety nature sterilization material to make, and safe droplet slide glass is divided into several region of the same area, and safe droplet slide glass is unified material integrated into one piece, freezing droplet comprises the special freezing protection liquid drop of fixed capacity, the liquid nitrogen is equipped with to the bottom in the liquid nitrogen steam drum to be full of liquid nitrogen steam in the jar, safe droplet slide glass is placed in freezing overcoat and is placed in liquid nitrogen steam drum. The utility model discloses an advantage embodies: adopt the bio -safety nature sterilization material of best thickness, the freezing sperm on this safe droplet slide glass is utilized to the high efficiency of being convenient for, safe droplet slide glass is unified material integrated into one piece, prevents that other materials from polluting, have regionalism, the better long -term storage of being convenient for is more preserved in liquid nitrogen steam and is prevented cross contamination.
Description
Technical field
The utility model is related to assisted reproduction field, and in particular to a kind of people's rareness sperm DPV freezings system of highly effective and safe
System.
Background technology
The infertility of current male factor account for 40-50%, and also have more serious development trend.It is more next
The incidence of disease of few azoospermia higher has a strong impact on the fecundity of male.Although we can be by testicular biopsy or micro-
Cut obtain sperm, but at present lack highly effective and safe freezen protective rareness sperm, particularly single sperm freezing carrier and
Method.
Chinese patent (A of CN 104823966) disclose a kind of micro sperm cryopreservation carrier and corresponding freezing and
Defreezing method, but its carrier medium is to lack security and lack region division and the intersection between sample cannot be prevented dirty
Dye.
Utility model content
The purpose of this utility model is directed to deficiency of the prior art, there is provided a kind of highly effective and safe and no cross contamination
People's rareness sperm (contains monosperm, such as testicular biopsy sperm, epididymis puncture sperm, Azoospermia patient sperm, testis is micro-
Cut sperm etc.) DPV refrigeration systems.
To achieve the above object, the utility model discloses following technical scheme：
A kind of people's rareness sperm DPV refrigeration systems of highly effective and safe, including freezing overcoat, safe droplet slide glass and liquid nitrogen steam
Vapour tank, the safe droplet slide glass is made of biological safety sterile material, and safe droplet slide glass is divided into several identical
The region of area, safe droplet slide glass is that unified material is integrally formed；Freeze special frozen solution of the droplet by fixed capacity
Drop is constituted, and freezing droplet drips on safe droplet slide glass when in use；The liquid nitrogen vapor tank inner bottom part be equipped with liquid nitrogen, and
Liquid nitrogen vapor is full of in tank, safe droplet slide glass is placed in freezing overcoat and is placed in liquid nitrogen vapor tank.
Further, the safe droplet slide glass is rectangular tab.
Further, the material of the safe droplet slide glass is thin using the biological safety polypropylene of ethylene oxide sterilizing
Piece.
Further, the thickness of the safe droplet slide glass is 1.5 μm.
Further, the safe droplet slide glass is divided into 8 regions of the same area.
Further, the safe droplet slide glass is provided with a handle for identical material, handle and safe droplet slide glass one
It is body formed.
Further, the liquid nitrogen liquid level that the liquid nitrogen vapor pot bottom is provided with is not higher than the height of freezing overcoat.
Further, support is provided with the liquid nitrogen vapor tank, safe droplet slide glass is placed in freezing overcoat and passes through
Support is placed in liquid nitrogen vapor tank.
People's rareness sperm that a further object of the present utility model is to provide a kind of highly effective and safe (contains monosperm, such as testis is worn
Thorn sperm, epididymis puncture micro- incision sperm of sperm, Azoospermia patient sperm, testis etc.) freezing and storing method, utilize
Above-mentioned DPV refrigeration systems, specifically include following steps：
The micro sperm freezing overcoats of S1：Drop has freezing droplet on safe droplet slide glass, is added in each freezing droplet corresponding
Sperm after, the safe droplet slide glass is put into the freezing overcoat matched with its size and is opened with the isolation of other sperm samples, so
The freezing overcoat for being loaded with safe droplet slide glass liquid nitrogen is carried out afterwards to fumigate；
The micro sperms of S2 are preserved for a long time：Prepare liquid nitrogen vapor tank of the bottom equipped with liquid nitrogen, and keep liquid nitrogen vapor
Liquid nitrogen vapor is full of in tank, the freezing overcoat that will be loaded with safe droplet slide glass is rack-mount, liquid nitrogen steaming is placed on for a long time
Preserved in vapour.
A kind of people's rareness sperm DPV refrigeration systems and freezing method of highly effective and safe disclosed in the utility model, with
Lower beneficial effect：
Safe droplet slide glass is easy to efficiently be carried using the safe droplet using the biological safety sterile material of optimum thickness
Frozen sperm on piece；
Safe droplet slide glass is that unified material is integrally formed, and can prevent other materials from polluting；
With region division, more preferably it is easy to long-term preservation, and the cross pollution between sample can be prevented.
Brief description of the drawings
Fig. 1 is the combination diagram of safe droplet slide glass and freezing overcoat；
Fig. 2 is the step of sperm sample is loaded into freezing droplet schematic diagram (1)；
Fig. 3 is the step of sperm sample is loaded into freezing droplet schematic diagram (2)
Fig. 4 is the step of micro sperm is preserved for a long time schematic diagram；
Fig. 5 is the step of taking out frozen sperm schematic diagram.
Description of reference numerals：
1. overcoat is freezed, 2. handle, 3. safe droplet slide glass, 4. sperm sample, 5. freeze droplet, 6. support, 7. liquid nitrogen
Vapor can, 8. liquid nitrogen.
Specific embodiment
With reference to embodiment and the utility model is further described referring to the drawings.
Refer to Fig. 1-Fig. 4.
A kind of people's rareness sperm DPV refrigeration systems of highly effective and safe, including freezing overcoat 1, safe droplet slide glass 3, freezing
Droplet 5 and liquid nitrogen vapor tank 7, the safe droplet slide glass 3 are made of biological safety sterile material, safe droplet slide glass 3
Several regions of the same area are divided into, are easy to efficiently using the frozen sperm on the freezing carrier, safe droplet slide glass 3 is
Unified material is integrally formed, and prevents other materials from polluting；Freezing droplet 5 is made up of the special cryoprotection drop of fixed capacity,
Freezing droplet 5 drips on safe droplet slide glass 3 when in use；The inner bottom part of liquid nitrogen vapor tank 7 is equipped with liquid nitrogen 8, and in tank
It is interior full of liquid nitrogen vapor, safe droplet slide glass 3 be placed on freezing overcoat 1 in and be placed in liquid nitrogen vapor tank 7.
Used as specific embodiment, the safe droplet slide glass 3 is Thin Rectangular slide glass.
Used as specific embodiment, the material of the safe droplet slide glass 3 is gathered using the biological safety of ethylene oxide sterilizing
Propylene thin slice, the carrier is the biological safety sterile material of optimum thickness.
Used as specific embodiment, the thickness of the safe droplet slide glass 3 is 1.5 μm.
Used as specific embodiment, the safe droplet slide glass 3 is divided into 8 regions of the same area.
Used as specific embodiment, the safe droplet slide glass 3 is provided with a handle for identical material 2, and handle 2 is micro- with safety
Drop slide glass 3 is integrally formed.
Used as specific embodiment, the liquid level of liquid nitrogen 8 that the bottom of liquid nitrogen vapor tank 7 is provided with is not higher than freezing overcoat 1
Height.
See Fig. 4, as specific embodiment, support 6 is provided with the liquid nitrogen vapor tank 7, safe droplet slide glass 3 is placed on
In freezing overcoat 1 and it is placed in liquid nitrogen vapor tank 7 by support 6.
A kind of people's rareness sperm cryopreservation method of highly effective and safe disclosed in the utility model, is freezed using above-mentioned DPV
System, comprises the following steps：
The micro sperm freezing overcoat of step 1：Drop has freezing droplet 5 on safe droplet slide glass 3, adds in each freezing droplet 5
After entering corresponding sperm, by the safe droplet slide glass 3 be put into the freezing overcoat 1 matched with its size with other sperm samples every
Open absolutely, the freezing overcoat 1 for being loaded with safe droplet slide glass 3 is then carried out liquid nitrogen fumigates.
The micro sperm of step 2 is preserved for a long time：Prepare liquid nitrogen vapor tank 7 of the bottom equipped with liquid nitrogen 8, and keep liquid nitrogen
Liquid nitrogen vapor is full of in vapor can 7, the freezing overcoat 1 that will be loaded with safe droplet slide glass 3 is installed on the frame 6, it is long-term to place
Preserved in liquid nitrogen vapor.
See Fig. 5, when frozen sperm sample is taken out, the freezing overcoat 1 with safe droplet slide glass 3 is taken out first, will pacify
Full droplet slide glass 3 is placed in sampling ware, and sampled instrument draws frozen sperm sample.
The utility model is easy to efficiently utilize the safe droplet slide glass using the biological safety sterile material of optimum thickness
On frozen sperm；Safe droplet slide glass is that unified material is integrally formed, and can prevent other materials from polluting；With region division,
More preferably it is easy to long-term preservation.
The above is only preferred embodiment of the present utility model, rather than its limitations；Although it should be pointed out that with reference to upper
Each embodiment is stated to be described in detail the utility model, it will be understood by those within the art that, it still can be with
Technical scheme described in the various embodiments described above is modified, or which part or all technical characteristic are equally replaced
Change；And these modifications and replacement, the essence of corresponding technical scheme is departed from each embodiment technical scheme of the utility model
Scope.
Claims (8)
1. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe, it is characterised in that carried including freezing overcoat, safe droplet
Piece and liquid nitrogen vapor tank, the safe droplet slide glass are made of biological safety sterile material, and safe droplet slide glass is divided
It is several regions of the same area, safe droplet slide glass is that unified material is integrally formed；Freezing droplet is by the special of fixed capacity
Cryoprotection drop is constituted, and freezing droplet drips on safe droplet slide glass when in use；The liquid nitrogen vapor tank inner bottom part is equipped with
Liquid nitrogen, and liquid nitrogen vapor is full of in tank, safe droplet slide glass is placed in freezing overcoat and is placed in liquid nitrogen vapor tank.
2. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
Safe droplet slide glass is rectangular tab.
3. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
The material of safe droplet slide glass uses the biological safety polypropylene sheet of ethylene oxide sterilizing.
4. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
The thickness of safe droplet slide glass is 1.5 μm.
5. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
Safe droplet slide glass is divided into 8 regions of the same area.
6. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
Safe droplet slide glass is provided with a handle for identical material, and handle is integrally formed with safe droplet slide glass.
7. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
The liquid nitrogen liquid level that liquid nitrogen vapor pot bottom is provided with is not higher than the height of freezing overcoat.
8. people's rareness sperm DPV refrigeration systems of a kind of highly effective and safe according to claim 1, it is characterised in that described
Support is provided with liquid nitrogen vapor tank, safe droplet slide glass is placed in freezing overcoat and is placed on liquid nitrogen vapor tank by support
It is interior.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620544372.5U CN206182196U (en) | 2016-06-06 | 2016-06-06 | Rare sperm DPV refrigeration system of high -efficient safe people |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620544372.5U CN206182196U (en) | 2016-06-06 | 2016-06-06 | Rare sperm DPV refrigeration system of high -efficient safe people |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206182196U true CN206182196U (en) | 2017-05-24 |
Family
ID=58737275
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620544372.5U Expired - Fee Related CN206182196U (en) | 2016-06-06 | 2016-06-06 | Rare sperm DPV refrigeration system of high -efficient safe people |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206182196U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021018292A1 (en) * | 2019-08-01 | 2021-02-04 | 常州市妇幼保健院 | Method for storing individual sperm or trace amount of sperm, and chip carrier |
-
2016
- 2016-06-06 CN CN201620544372.5U patent/CN206182196U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021018292A1 (en) * | 2019-08-01 | 2021-02-04 | 常州市妇幼保健院 | Method for storing individual sperm or trace amount of sperm, and chip carrier |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170524 Termination date: 20200606 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |