CN105850986A - Efficient and safe DPV freezing system and method for human oligoasthenozoospermia - Google Patents
Efficient and safe DPV freezing system and method for human oligoasthenozoospermia Download PDFInfo
- Publication number
- CN105850986A CN105850986A CN201610396509.1A CN201610396509A CN105850986A CN 105850986 A CN105850986 A CN 105850986A CN 201610396509 A CN201610396509 A CN 201610396509A CN 105850986 A CN105850986 A CN 105850986A
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- Prior art keywords
- safe
- slide glass
- freezing
- liquid nitrogen
- droplet
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
Abstract
The invention relates to an efficient and safe DPV freezing system for human oligoasthenozoospermia. The system comprises a safe microdroplet slide glass, freezing microdroplets and a liquid nitrogen steam tank. The safe microdroplet slide glass is made from biological safety sterilization materials, and the safe microdroplet slide glass is divided into multiple regions of the same area, and the safe microdroplet slide glass is integrally formed from unified materials. The freezing microdroplets are composed of special freezing protective droplets with the fixed volume. Liquid nitrogen is contained at the bottom inside the liquid nitrogen steam tank, and the tank is filled with liquid nitrogen steam. The safe microdroplet slide glass is placed in a freezing sleeve and placed in the liquid nitrogen steam tank. The invention further relates to a DPV freezing method for human oligoasthenozoospermia. The efficient and safe DPV freezing system has the advantages that the biological safety sterilization materials with the best thickness are adopted, frozen sperms on the safe microdroplet slide glass can be efficiently utilized, and the safe microdroplet slide glass is integrally formed from the unified materials so as to prevent pollution of other materials; regional division is achieved, long-term preservation is more convenient, sperms can be preserved in liquid nitrogen steam more easily, and cross contamination is avoided.
Description
Technical field
The present invention relates to assisted reproduction field, be specifically related to people's rareness sperm of a kind of highly effective and safe
DPV refrigeration system and freezing method.
Background technology
The infertility of male factor account for 40-50% at present, and also has more serious
Development trend.The incidence of disease of the highest few azoospermia has a strong impact on the fecundity of the male sex.
Although we can obtain sperm by testicular biopsy or micro-incision, but lacks height at present
The freezen protective rareness sperm of effect safety, the most single sperm freezing carrier and method.
Chinese patent (CN 104823966A) discloses a kind of trace sperm cryopreservation carrier
And corresponding freezing and defreezing method, but its carrier medium is for lacking security and lacking region
Divide and cannot effectively prevent cross pollution between sample.
Summary of the invention
It is an object of the invention to for deficiency of the prior art, it is provided that a kind of highly effective and safe is without handing over
People's rareness sperm that fork pollutes (containing monosperm, as testicular biopsy sperm, epididymis puncture sperm,
Azoospermia patient's sperm, testis micro-incision sperm etc.) DPV refrigeration system.
For achieving the above object, the invention discloses following technical scheme:
A kind of people rareness sperm DPV refrigeration system of highly effective and safe, including safe droplet slide glass,
Freezing droplet and liquid nitrogen vapor tank, described safe droplet slide glass uses biological safety sterile material
Making, safe droplet slide glass is divided into several region of the same area, and safe droplet slide glass is
Unified material is one-body molded；Described freezing droplet is by the special cryoprotection drop structure of fixed capacity
Becoming, freezing droplet drips in use on safe droplet slide glass；Described liquid nitrogen vapor tank inner bottom part
Equipped with liquid nitrogen, and being full of liquid nitrogen vapor in tank, safe droplet slide glass is placed on freezing overcoat
In and be placed in liquid nitrogen vapor tank.
Further, described safe droplet slide glass is rectangular tab.
Further, the material of described safe droplet slide glass uses the biological peace of ethylene oxide sterilizing
Full property polypropylene sheet.
Further, the thickness of described safe droplet slide glass is 1.5 μm.
Further, described safe droplet slide glass is divided into 8 regions of the same area.
Further, described safe droplet slide glass is provided with the handle of an identical material, handle with
Safe droplet slide glass is one-body molded.
Further, the liquid nitrogen liquid level that described liquid nitrogen vapor pot bottom is provided with not higher than freezing
The height of overcoat.
Further, being provided with support in described liquid nitrogen vapor tank, safe droplet slide glass is placed on cold
In freezing overcoat and it is placed in liquid nitrogen vapor tank by support.
Another object of the present invention be to provide a kind of highly effective and safe people's rareness sperm (containing monosperm,
Such as testicular biopsy sperm, epididymis, to puncture sperm, Azoospermia patient's sperm, testis micro-
Cut sperm etc.) freezing and storing method, utilize above-mentioned DPV refrigeration system, specifically include as
Lower step:
S1 trace sperm freezing overcoat: dripping on safe droplet slide glass and have freezing droplet, each is cold
Freeze after droplet adds corresponding sperm, this safe droplet slide glass put into and mates with its size
Freezing overcoat is opened, then being loaded with the cold of safe droplet slide glass with the isolation of other sperm samples
Freeze overcoat carry out liquid nitrogen fumigate；
S2 trace sperm preserves for a long time: prepare the bottom liquid nitrogen vapor tank equipped with liquid nitrogen,
And it is full of liquid nitrogen vapor in keeping liquid nitrogen vapor tank, the freezing of safe droplet slide glass will be loaded with
Overcoat is rack-mount, is placed in liquid nitrogen vapor for a long time and preserves.
People's rareness sperm DPV refrigeration system of a kind of highly effective and safe disclosed by the invention and freezing
Method, has the advantages that
Safe droplet slide glass uses the biological safety sterile material of optimum thickness, it is simple to efficiently profit
With the frozen sperm on this safe droplet slide glass；
Safe droplet slide glass is one-body molded for unified material, can prevent other materials from polluting；
There is region divide, be more preferably easy to preserve for a long time, and cross pollution between sample can be prevented.
Accompanying drawing explanation
Fig. 1 is the combination schematic diagram of safe droplet slide glass and freezing overcoat；
Fig. 2 is the step schematic diagram (1) that sperm sample loads freezing droplet；
Fig. 3 is the step schematic diagram (2) that sperm sample loads freezing droplet
Fig. 4 is the step schematic diagram that trace sperm preserves for a long time；
Fig. 5 is the step schematic diagram taking out frozen sperm.
Description of reference numerals:
1. freezing overcoat, 2. handle, the safest droplet slide glass, 4. sperm sample, 5. freezing micro-
Drip, 6. support, 7. liquid nitrogen vapor tank, 8. liquid nitrogen.
Detailed description of the invention
Below in conjunction with embodiment and referring to the drawings the invention will be further described.
Refer to Fig. 1-Fig. 4.
A kind of people rareness sperm DPV refrigeration system of highly effective and safe, including safe droplet slide glass 1,
Freezing droplet 5 and liquid nitrogen vapor tank 7, described safe droplet slide glass 3 uses biological safety to go out
Bacterium material is made, and safe droplet slide glass 3 is divided into several region of the same area, it is simple to high
Effect utilizes the frozen sperm on this freezing carrier, and safe droplet slide glass 3 becomes for unified material one
Type, prevents other materials from polluting；Described freezing droplet 5 is by the special cryoprotection of fixed capacity
Drop is constituted, and freezing droplet 5 drips in use on safe droplet slide glass 3；Described liquid nitrogen steams
Vapour tank 7 inner bottom part is equipped with liquid nitrogen 8, and is full of liquid nitrogen vapor, safe droplet slide glass in tank
In 3 are placed on freezing overcoat 1 and be placed in liquid nitrogen vapor tank 7.
As specific embodiment, described safe droplet slide glass 3 is Thin Rectangular slide glass.
As specific embodiment, the material of described safe droplet slide glass 3 uses ethylene oxide sterilizing
Biological safety polypropylene sheet, this carrier is the biological safety sterile material of optimum thickness.
As specific embodiment, the thickness of described safe droplet slide glass 3 is 1.5 μm.
As specific embodiment, described safe droplet slide glass 3 be divided into 8 of the same area
Region.
As specific embodiment, described safe droplet slide glass 3 is provided with the handle of an identical material
2, handle 2 is one-body molded with safe droplet slide glass 3.
As specific embodiment, liquid nitrogen 8 liquid level being provided with bottom described liquid nitrogen vapor tank 7
The height of the most freezing overcoat 1.
See Fig. 4, as specific embodiment, in described liquid nitrogen vapor tank 7, be provided with support 6, peace
It is placed on liquid nitrogen vapor tank 7 in full droplet slide glass 3 is placed on freezing overcoat 1 and by support 6
In.
People's rareness sperm cryopreservation method of a kind of highly effective and safe disclosed by the invention, in utilization
State DPV refrigeration system, comprise the steps:
Step 1 trace sperm freezing overcoat: drip on safe droplet slide glass 3 and have freezing droplet 5,
After each freezing droplet 5 adds corresponding sperm, this safe droplet slide glass 3 is put into and it
The freezing overcoat 1 of size coupling is opened, then being loaded with safety with the isolation of other sperm samples
The freezing overcoat 1 of droplet slide glass 3 carries out liquid nitrogen and fumigates.
Step 2 trace sperm preserves for a long time: prepare the liquid nitrogen steaming equipped with liquid nitrogen 8 of the bottom
Vapour tank 7, and keep being full of in liquid nitrogen vapor tank 7 liquid nitrogen vapor, safe droplet will be loaded with
The freezing overcoat 1 of slide glass 3 is installed on the frame 6, is placed in liquid nitrogen vapor for a long time and protects
Deposit.
See Fig. 5, when taking out frozen sperm sample, first take out with safe droplet slide glass 3
Freezing overcoat 1, safe droplet slide glass 3 is placed in sampling ware in, sampled instrument draw in cold
Freeze sperm sample.
The present invention uses the biological safety sterile material of optimum thickness, it is simple to efficiently utilize this peace
Frozen sperm on full droplet slide glass；Safe droplet slide glass is one-body molded for unified material, can prevent
Only other materials pollutes；There is region divide, be more preferably easy to preserve for a long time.
The above is only the preferred embodiment of the present invention, is not intended to limit；It should be pointed out that,
Although the present invention being described in detail with reference to the various embodiments described above, the ordinary skill of this area
Personnel should be appreciated that the technical scheme described in the various embodiments described above still can be repaiied by it
Change, or the most some or all of technical characteristic is carried out equivalent；And these are revised and replace
Change, do not make the essence of corresponding technical scheme depart from the model of various embodiments of the present invention technical scheme
Enclose.
Claims (9)
1. people's rareness sperm DPV refrigeration system of a highly effective and safe, it is characterised in that include
Safe droplet slide glass, freezing droplet and liquid nitrogen vapor tank, described safe droplet slide glass uses biology
Security sterile material is made, and safe droplet slide glass is divided into several region of the same area,
Safe droplet slide glass is one-body molded for unified material；Special by fixed capacity of described freezing droplet
Cryoprotection drop is constituted, and freezing droplet drips in use on safe droplet slide glass；Described liquid
Nitrogen vapor tank inner bottom part is equipped with liquid nitrogen, and is full of liquid nitrogen vapor, safe droplet slide glass in tank
In being placed on freezing overcoat and be placed in liquid nitrogen vapor tank.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that described safe droplet slide glass is rectangular tab.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that the material of described safe droplet slide glass uses the biology of ethylene oxide sterilizing
Security polypropylene sheet.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that the thickness of described safe droplet slide glass is 1.5 μm.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that described safe droplet slide glass is divided into 8 regions of the same area.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that described safe droplet slide glass is provided with the handle of an identical material, handle
One-body molded with safe droplet slide glass.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that the liquid nitrogen liquid level that described liquid nitrogen vapor pot bottom is provided with is the coldest
Freeze the height of overcoat.
The people rareness sperm DPV freezing system of a kind of highly effective and safe the most according to claim 1
System, it is characterised in that being provided with support in described liquid nitrogen vapor tank, safe droplet slide glass is placed on
In freezing overcoat and it is placed in liquid nitrogen vapor tank by support.
9. people's rareness sperm cryopreservation method of a highly effective and safe, it is characterised in that utilize
DPV refrigeration system as described in claim 1-8, comprises the steps:
S1 trace sperm freezing overcoat: dripping on safe droplet slide glass and have freezing droplet, each is cold
Freeze after droplet adds corresponding sperm, this safe droplet slide glass put into and mates with its size
Freezing overcoat is opened, then being loaded with the cold of safe droplet slide glass with the isolation of other sperm samples
Freeze overcoat carry out liquid nitrogen fumigate；
S2 trace sperm preserves for a long time: prepare the bottom liquid nitrogen vapor tank equipped with liquid nitrogen,
And it is full of liquid nitrogen vapor in keeping liquid nitrogen vapor tank, the freezing of safe droplet slide glass will be loaded with
Overcoat is rack-mount, is placed in liquid nitrogen vapor for a long time and preserves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610396509.1A CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610396509.1A CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105850986A true CN105850986A (en) | 2016-08-17 |
| CN105850986B CN105850986B (en) | 2019-03-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610396509.1A Active CN105850986B (en) | 2016-06-06 | 2016-06-06 | A kind of the people's rareness sperm DPV refrigeration system and freezing method of highly effective and safe |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021018292A1 (en) * | 2019-08-01 | 2021-02-04 | 常州市妇幼保健院 | Method for storing individual sperm or trace amount of sperm, and chip carrier |
| CN113040132A (en) * | 2021-03-22 | 2021-06-29 | 浙江大学 | Sperm cryopreservation method based on protective agent |
| CN113712025A (en) * | 2021-09-14 | 2021-11-30 | 荆州市中心医院 | Cryopreservation method of trace sperms and preparation method of sperms directly used for ICSI |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671651A (en) * | 2008-09-09 | 2010-03-17 | 上海交通大学医学院附属第九人民医院 | Sperm freezing and unfreezing method and sperm freezing and unfreezing device |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN204888506U (en) * | 2015-07-22 | 2015-12-23 | 柳州市妇幼保健院 | Efficient biomaterial freezes and deposits carrier device |
-
2016
- 2016-06-06 CN CN201610396509.1A patent/CN105850986B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671651A (en) * | 2008-09-09 | 2010-03-17 | 上海交通大学医学院附属第九人民医院 | Sperm freezing and unfreezing method and sperm freezing and unfreezing device |
| CN202514486U (en) * | 2012-02-16 | 2012-11-07 | 上海安集协康生物技术有限公司 | Container suitable for liquid nitrogen tank to store cells |
| CN104823966A (en) * | 2015-05-19 | 2015-08-12 | 上海交通大学医学院附属第九人民医院 | Trace sperm cryopreservation carrier and corresponding freezing and thawing methods thereof |
| CN204888506U (en) * | 2015-07-22 | 2015-12-23 | 柳州市妇幼保健院 | Efficient biomaterial freezes and deposits carrier device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021018292A1 (en) * | 2019-08-01 | 2021-02-04 | 常州市妇幼保健院 | Method for storing individual sperm or trace amount of sperm, and chip carrier |
| CN113040132A (en) * | 2021-03-22 | 2021-06-29 | 浙江大学 | Sperm cryopreservation method based on protective agent |
| CN113040132B (en) * | 2021-03-22 | 2022-03-25 | 浙江大学 | Sperm cryopreservation method based on protective agent |
| CN113712025A (en) * | 2021-09-14 | 2021-11-30 | 荆州市中心医院 | Cryopreservation method of trace sperms and preparation method of sperms directly used for ICSI |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105850986B (en) | 2019-03-12 |
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