CN105850901A - Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system - Google Patents
Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system Download PDFInfo
- Publication number
- CN105850901A CN105850901A CN201610239525.XA CN201610239525A CN105850901A CN 105850901 A CN105850901 A CN 105850901A CN 201610239525 A CN201610239525 A CN 201610239525A CN 105850901 A CN105850901 A CN 105850901A
- Authority
- CN
- China
- Prior art keywords
- silkworm
- ammonia
- concentration
- breeding environment
- ammonia concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 289
- 241000255789 Bombyx mori Species 0.000 title claims abstract description 256
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 142
- 238000009395 breeding Methods 0.000 title claims abstract description 89
- 230000001488 breeding effect Effects 0.000 title claims abstract description 89
- 238000011161 development Methods 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 23
- 235000013305 food Nutrition 0.000 claims description 40
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 31
- 230000000384 rearing effect Effects 0.000 claims description 20
- 235000008708 Morus alba Nutrition 0.000 claims description 19
- 240000000249 Morus alba Species 0.000 claims description 18
- 238000011156 evaluation Methods 0.000 claims description 11
- 241000382353 Pupa Species 0.000 claims description 5
- 239000000523 sample Substances 0.000 claims description 4
- 201000010099 disease Diseases 0.000 abstract description 27
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 27
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000012544 monitoring process Methods 0.000 abstract description 2
- 238000005259 measurement Methods 0.000 abstract 1
- 239000007952 growth promoter Substances 0.000 description 12
- 206010040047 Sepsis Diseases 0.000 description 11
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 10
- 239000004033 plastic Substances 0.000 description 9
- 229920003023 plastic Polymers 0.000 description 9
- 238000011160 research Methods 0.000 description 7
- 239000011521 glass Substances 0.000 description 6
- 244000052769 pathogen Species 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 241000145903 Bombyx mori cypovirus 1 Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 241000255791 Bombyx Species 0.000 description 3
- 239000000090 biomarker Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 238000009366 sericulture Methods 0.000 description 3
- 241001126828 Nosema bombycis Species 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 238000009941 weaving Methods 0.000 description 2
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000255794 Bombyx mandarina Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000218213 Morus <angiosperm> Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009361 aviculture Methods 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000003181 biological factor Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/04—Silkworms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/0054—Ammonia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Combustion & Propulsion (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses detection of concentration of ammonia in a breeding environment and application thereof in establishing a silkworm growth and development judgment system, and a novel technology for monitoring and performing early warning on silkworm breeding conditions through measurement of concentration of ammonia in the breeding environment is established on the basis. According to the scheme, the silkworm breeding conditions can be prejudged by detecting concentration of ammonia in the breeding environment, and even silkworm disease hazard conditions can be judged preliminarily, a reference index and method for forecasting and performing early warning on the silkworm breeding conditions are provided for the silkworm-raising industry by detecting the concentration of ammonia in a silkworm house, growth conditions and breeding benefits of silkworms can be predicted, theoretical guidance is provided for actual work, and the method is simple and convenient and has wide application prospects.
Description
Technical field
The invention belongs to silkworm cultural technique field.More particularly, to the detection of ammonia concentration in breeding environment
And the application in setting up silkworm growth promoter evaluation system.
Background technology
Silkworm (Bombyx mori) is a kind of special economic animal, is that weaving silk with Folium Mori as foodstuff is cocoond
One of economic insects, silkworm larva eats Folium Mori, and spuing before pupating and doing a large amount of silkworm silks of cocoon is important weaving
Raw material.The mankind are initially to gather original wild Bombyx bombycis from mulberry trees to take silk utilization；Along with human lives settle down and
Silkworm silk purposes is further appreciated that and starts in indoor sericulture.Through long-term cultivation and selection, wild silkworm is
Gradually domestication becomes the Silkworm with such special economic character today.
The silkworm raised and train through more than 5,000 years is higher to the requirement of feeding environment, in breeding environment biological factor and
Abiotic factor all can produce impact in various degree to the growth promoter of silkworm.Therefore, relevant to silkworm cultivation
In environment, between various possible influence factors and Growth And Development of Silkworm, the research of relation is respectively provided with important scientific research
With production meaning.
Ammonia is one of gas componant of cocoonery, advises at " Indoor Air Quality standards " (GB/T18883-2002)
Having determined ammonia maximum permissible concentration in room air is 0.2mg/m3(closing 0,2635ppm), but at breeding process
In, the ammonia of cocoonery is often gradually increasing, and the ammonia of cocoonery is essentially from the gastrointestinal disturbances of silkworm, silkworm faeces, Mulberry
Organic substance corruption, antibacterial or other pathogenic microorganisms such as leaf residue decompose body waste and are produced.In aquaculture
Have relevant research to show, the excessive concentration of ammonia the growth of broiler can be affected (king's rice etc.,
Ammonia to the harm of aviculture and anti-system, Chinese poultry resource, 2006,28 (18): 27 28).At present, ammonia is with foster
Between silkworm industry and Growth And Development of Silkworm, the correlational study of relation is less, easily ignores ammonia provisions in sericulture
The harm that silkworm brings.
Summary of the invention
The technical problem to be solved in the present invention is to overcome in prior art ammonia concentration and family in silkworm breeding environment
Between silkworm growth promoter, the research of relation is not enough, specify that in breeding environment ammonia concentration and Growth And Development of Silkworm it
Between relation, it is provided that in breeding environment ammonia concentration detection and in setting up silkworm growth promoter evaluation system
Application.The present invention utilizes ammonia concentration to be a kind of technological innovation as a kind of reference index of silkworm rearing situation.
It is an object of the invention to provide ammonia concentration application in setting up Growth And Development of Silkworm evaluation system.
Another object of the present invention is to provide one and identifies silkworm rearing by measuring ammonia concentration in breeding environment
Situation quality and the new technique of prediction silkworm disease.Ammonia in cocoonery is detected particular by ammonia detector
Concentration value predict the raising situation of silkworm and disease a situation arises.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
Ammonia concentration application in the evaluation system setting up silkworm rearing situation quality in breeding environment.Can basis
In breeding environment, the change of ammonia concentration sets up silkworm rearing feelings with the corresponding relation of silkworm cultivation situation quality
The evaluation system of condition quality.
The most specifically, described application refers to that in breeding environment, ammonia concentration is raised in prediction and/or judge silkworm
Support the application in terms of situation quality.
More specifically, described silkworm rearing situation quality refers to the quality of Growth And Development of Silkworm situation.
Preferably, the quality of described Growth And Development of Silkworm situation refers to the growth rate of silkworm, food Mulberry amount, cocoon layer
The height of rate and/or worm pupa vital rates etc..
In above-mentioned application, in inverse relation between described ammonia concentration and silkworm biological characteristics.I.e. ammonia is dense
The biological characteristics (growth rate, food Mulberry amount, Cocoon layer ratio, worm pupa vital rates etc.) of the high then silkworm of degree is low；Ammonia
Gas concentration is low, represents that the biological characteristics of silkworm is high, i.e. growth promoter is good, growth rate is high, food Mulberry amount is big,
Cocoon layer ratio is high, worm pupa vital rates is high.
Specifically, the standard of above-mentioned prediction and/or judge is:
When in silkworm breeding environment, ammonia concentration is when less than 3.00ppm, and the biological characteristics of silkworm is the highest, raw
Long development condition is fine；
When in silkworm breeding environment, ammonia concentration is between 3.00～8.00ppm, the biological characteristics of silkworm is relatively
Height, growth and development state is preferable；
When in silkworm breeding environment, ammonia concentration is between 8.00～15.00ppm, the biological characteristics of silkworm
Relatively low, growth and development state is affected by certain, relatively low；
When in silkworm breeding environment, ammonia concentration is between 15.00～25.00ppm, the biological characteristics of silkworm
Lower, growth and development state is affected by more serious.
When in silkworm breeding environment, ammonia concentration, when more than 25.00ppm, has shown the breeding environment of silkworm
Very severe, is not suitable for the growth promoter of silkworm very much.
Further, pass between ammonia concentration and silkworm disease during the present invention is investigated silkworm breeding environment
System, result shows, ammonia concentration has certain relation, real work with silkworm disease the most to a certain extent
In, the possible susceptible situation of silkworm, and the disease that silkworm is infected can be predicted according to ammonia concentration in breeding environment
Evil kind carries out preliminary diagnosis.
I.e. the invention also discloses ammonia concentration in silkworm breeding environment predicting and the susceptible situation side of early warning silkworm
The application in face.
The present invention is through substantial amounts of research and exploration, it is also expressly that concrete ammonia concentration and silkworm dissimilar disease
Relation between disease, particularly as follows:
When in silkworm breeding environment, ammonia concentration is between 3.00～6.00ppm；Measurable silkworm has infected house
Nosematosis, after infection pebrine, the ammonia concentration ascendant trend in silkworm room is inconspicuous；
When in silkworm breeding environment, ammonia concentration is between 8.00～15.00ppm, measurable silkworm infects
Bacillary septicaemia, after bacterial infection septicaemia, the ammonia concentration early stage ascendant trend in silkworm room is delayed
Slowly, later stage ammonia concentration ascendant trend is more apparent；
When in silkworm breeding environment, ammonia concentration is between 15.00～25.00ppm, measurable silkworm infects
Silkworm cytoplasmic polyhedrosis virus, infects the ammonia concentration rate of climb in silkworm room after this virus fast.
Therefore, in breeding environment, ammonia concentration can also be applied to predict the susceptible situation of silkworm, even predicts mirror
The classification that other silkworm is susceptible.
According to above-mentioned discovery, we have proposed one and monitor and early warning by measuring ammonia concentration in breeding environment
The new technique of silkworm rearing situation quality, is the ammonia concentration in detection silkworm breeding environment, ammonia from low to high
Gas concentration has indicated respectively by the good silkworm rearing situation to difference.
Specifically, as a kind of preferable embodiment, above-mentioned New technical use cultivates work in actual silkworm
Time in work, ammonia detector can be equipped with in silkworm breeding environment, utilize in ammonia detector detection breeding environment
Ammonia concentration, different alarm songs can be sent when ammonia detector detects the ammonia of variable concentrations.
Specifically, when the ammonia concentration that ammonia detector detects is less than 3.00ppm, ammonia detector can be sent out
Go out Pyatyi alarm；
When the ammonia concentration that ammonia detector detects is 3.00～8.00ppm, ammonia detector can send four
Level alarm；
When the ammonia concentration that ammonia detector detects is 8.00～15.00ppm, ammonia detector can send
Three grades of alarms；
When the ammonia concentration that ammonia detector detects is 15.00～25.00ppm, ammonia detector can send
Second-level alarm；
When the ammonia concentration that ammonia detector detects is more than 25.00ppm, ammonia detector can send one-level
Alarm.
Alert Level the highest (one-level is highest level), represents that the growth and development state of silkworm is worst, actual work
In work, can take different corresponding measures according to different alarms, improve cultivation situation further, investigation is not in time
Profit factor, to avoid unnecessary loss.
Therefore, the application in terms of judge and/or monitoring and early warning silkworm rearing situation quality of the above-mentioned new technique,
Also within protection scope of the present invention.
It addition, above-mentioned new technique can also be applied to prediction and the susceptible situation of early warning silkworm, even predict mirror
The classification that other silkworm is susceptible, i.e. the invention also discloses above-mentioned new technique in prediction and the susceptible situation of early warning silkworm
The application of aspect.In real work, can be pre-according to different alarms (ammonia concentration in instruction breeding environment)
Survey the possible susceptible situation of silkworm, and the disease species infecting silkworm carries out preliminary diagnosis, and can be further
Take prophylactico-therapeutic measures, timely symptom management.
The research contents of the present invention mainly includes following two large divisions:
(1) the raising situation of normal silkworm is judged by cocoonery ammonia concentration.The life of the high then silkworm of ammonia concentration
Thing characteristic (growth rate, food Mulberry amount, Cocoon layer ratio etc.) is low, the biological characteristics of the low then silkworm of ammonia concentration
High.
(2) by the silkworm after adding food Different Kinds of Pathogens is carried out ammonia concentration mensuration, according to infecting different epidemic disease
The ammonia concentration of silkworm judges the kind of disease.Add food silkworm cytoplasmic polyhedrosis virus (Bombyx mori
Cytoplasmic polyhedrosis virus, is called for short BmCPV) silkworm ammonia concentration advance the speed the fastest, dense
Spend the highest.And add food nosema bombycis disease pathogen micropartical spore Nosema bombycis (being called for short N.b)
Silkworm ammonia concentration advance the speed slowly, ammonia concentration is the lowest, adds the silkworm eating bacillary septicaemia cause of disease
Ammonia concentration early stage increases slowly, and later stage increment rate substantially strengthens.
It addition, the present invention can produce NH through numerous studies and exploration discovery, Bombyx mori L. and dead silkworm corpse3, silkworm
It is the most that sand accumulates, NH3Content is the highest；Dead silkworm corpse is the most more rotten, NH3Content is the highest.And about sense
Catch an illness former aspect, found by comparative study, add the NH of food N.b silkworm3Concentration is all than other two kinds of diseases
Former low, reason is that the quantity adding and eating N.b silkworm is fewer and feweri, and Bombyx mori L. amount, food Mulberry amount is low, and silkworm after death will not horse
Upper rotten；Adding the silkworm that food septicaemia is former, when silkworm is ripe, sickness rate is the highest, but owing to silkworm number is many, food
Mulberry amount is big, and Bombyx mori L. is many, so NH3Concentration is high；Owing to adding food CPV silkworm rapid onset, dead silkworm rots, institute
With NH3Concentration gathers way soon.
According to this result of study, in real work, can take respectively periodically according to different Alert Levels
Clear up Bombyx mori L. and dead silkworm corpse in time, improve environment ventilation ventilation condition, reasonable Symptomatic medicine etc. measure.
It addition, the result of study of the present invention shows, the change of the ozone concentration in silkworm breeding environment also with silkworm
Upgrowth situation, the most susceptible situation, have certain relation, therefore the ozone concentration in breeding environment can be made
It is an auxiliary characteristics, in conjunction with the ammonia concentration in breeding environment, to Growth And Development of Silkworm situation and susceptible situation
It is monitored and early warning.
Therefore, ozone concentration answering in the evaluation system setting up silkworm rearing situation quality in silkworm breeding environment
With, also within protection scope of the present invention.
The most specifically, described application refers to that in breeding environment, ammonia concentration is raised in prediction and/or judge silkworm
Support the application in terms of situation quality.
More specifically, described silkworm rearing situation quality refers to the quality of Growth And Development of Silkworm situation.
Preferably, the quality of described Growth And Development of Silkworm situation refer to silkworm growth rate, food Mulberry amount and/or
The height of Cocoon layer ratio etc..
In above-mentioned application, in inverse relation between described ammonia concentration and silkworm biological characteristics.I.e. ammonia is dense
The biological characteristics (growth rate, food Mulberry amount, Cocoon layer ratio etc.) of the high then silkworm of degree is low；The low then table of ammonia concentration
Show that the biological characteristics of silkworm is high, i.e. growth promoter is good, and growth rate is high, Mulberry amount is big, Cocoon layer ratio is high for food.
It addition, silkworm rearing situation quality is being set up in ammonia concentration and ozone concentration combination in silkworm breeding environment
Application in evaluation system, also within protection scope of the present invention.
And, above-mentioned by measure ammonia concentration in breeding environment monitor good with early warning silkworm rearing situation
In bad new technique, it is also possible in silkworm breeding environment, ozone concentration is as auxiliary characteristics, with ammonia concentration this
One index with the use of, indicate the quality of silkworm rearing situation.
Specifically, in breeding environment, ozone concentration is as auxiliary characteristics, predicts and evaluates silkworm development situation
Standard be:
When in silkworm breeding environment, ozone concentration is when less than 0.10ppm, and the biological characteristics of silkworm is the highest；
When in silkworm breeding environment, ozone concentration is between 0.10～0.30ppm, the biological characteristics of silkworm is relatively
High；
When in silkworm breeding environment, ozone concentration is between 0.30～0.50ppm, the biological characteristics of silkworm is relatively
Low；
When in silkworm breeding environment, ozone concentration is between 0.50～0.60ppm, the biological characteristics of silkworm is more
Low.
When in silkworm breeding environment, ozone concentration, when more than 0.60ppm, shows that the breeding environment of silkworm is the most non-
The most severe, it is not suitable for very much the growth promoter of silkworm.
The method have the advantages that
The present invention studies discovery, the biological characteristics such as the growth and development state of ammonia concentration and silkworm in breeding environment
There is critically important relation, even also there is with disease dependency, it can be determined that the susceptible situation of prediction silkworm, very
To susceptible kind, can be used to growing state and the culture benefit judging to predict silkworm, provide theory to refer to for real work
Lead and medication reference index.
Meanwhile, present invention research also finds, the change of the ozone concentration in silkworm breeding environment also with the life of silkworm
Long situation, the most susceptible situation, there is certain relation, therefore the ozone concentration in breeding environment can be as prison
One auxiliary characteristics of control early warning silkworm breeding situations.
According to the solution of the present invention, can be reflected by the ammonia concentration in detection breeding environment and/or ozone concentration
Fixed and the raising situation of prediction silkworm, it is judged that a situation arises for flacherie, it may be judged whether infect epidemic disease and susceptible
Type, provides a kind of reference index being identified silkworm disease by detection cocoonery ammonia concentration for sericulture.
Accompanying drawing explanation
Fig. 1 is NH in the silkworm breeding environment adding food Different Kinds of Pathogens3Concentrations versus's curve chart.
Detailed description of the invention
Further illustrate the present invention below in conjunction with Figure of description and specific embodiment, but embodiment is not to this
Invention limits in any form.Unless stated otherwise, the present invention uses reagent, method and apparatus are this skill
Art field conventional reagent, method and apparatus, following example agents useful for same and material are commercial.
Embodiment 1 detects ammonia concentration and the biological characteristics of silkworm of different raising situation health silkworm
1, material
Silkworm varieties: (only explanation the inventive method uses two-way cross kind " 7532 × Hunan sunshine ", it is possible to use
Other laboratorys or the upper conventional Silkworm varieties used of production, including original seed and the first generation of hybrid etc.), specification is 28
The plastic casing 3 with venthole that cm × 18cm × 10cm seals, specification is 27cm × 22cm × 22cm
3, the glass chest with venthole sealed, GT-2000 composite gas analyser.
2, method
(1) respectively take 50 four-aged silkworms and be put in plastic casing and glass chest raising, except two kinds of feeding environments
Volume difference outside, other rearing conditions is the most identical.
(2) the every morning 9:00AM probe by venthole gas detector, respectively 6 examinations of detection
Test the gas change of silkworm.
(3), after having surveyed gas, body weight and the food Mulberry amount of every box silkworm is claimed respectively；Silkworm pupate after claim cocoon shell weight, system
The biological characteristicses such as meter worm pupa vital rates.
3, experimental result
The ammonia concentration of table 1 plastic casing and the relation of silkworm biological characteristics
Note: "/" represents and do not claims to eat Mulberry amount
The ammonia concentration of table 2 glass chest and the relation of silkworm biological characteristics
Note: "/" represents and do not claims to eat Mulberry amount
Table 3 Bombyx bombycis weight statistical table
The biological indicator of table 4 silkworm
Note: result above is the meansigma methods of 3 experimental grouies
From the point of view of experimental result, the ammonia concentration of plastic casing is higher than the ammonia concentration of glass chest, from the life of silkworm
Thing characteristic understands, and the cultivation situation of the plastic casing silkworm that ammonia concentration is high does not has the glass chest that ammonia concentration is low
The cultivation situation of silkworm is good, the biological indicator significant difference of each silkworm.
The most in sum, can using the ammonia concentration in silkworm breeding environment as Testing index, predict and
Evaluate the cultivation situation quality of silkworm, especially for growth promoter shapes such as growth rate, food Mulberry amount, Cocoon layer ratio
The biological characteristics of condition.
Specifically, the standard of above-mentioned prediction and evaluation is:
When in silkworm breeding environment, ammonia concentration is when less than 3.00ppm, and the biological characteristics of silkworm is the highest, raw
Long development condition is fine；
When in silkworm breeding environment, ammonia concentration is between 3.00～8.00ppm, the biological characteristics of silkworm is relatively
Height, growth and development state is preferable；
When in silkworm breeding environment, ammonia concentration is between 8.00～15.00ppm, the biological characteristics of silkworm
Relatively low, growth and development state is affected by certain, relatively low；
When in silkworm breeding environment, ammonia concentration is between 15.00～25.00ppm, the biological characteristics of silkworm
Lower, growth and development state is affected by more serious.
When in silkworm breeding environment, ammonia concentration, when more than 25.00ppm, has shown the breeding environment of silkworm
Very severe, is not suitable for the growth promoter of silkworm very much.
Embodiment 2 detects ozone concentration and the biological characteristics of silkworm of different raising situation health silkworm
1, experimental technique is with embodiment 1.
2, result is as shown in table 5,6,7 and 8.
The ozone concentration of table 5 plastic casing and the relation of silkworm biological characteristics
Note: "/" represents and do not claims to eat Mulberry amount
The ozone concentration of table 6 glass chest and the relation of silkworm biological characteristics
Note: "/" represents and do not claims to eat Mulberry amount
Table 7 Bombyx bombycis weight statistical table
The biological indicator of table 8 silkworm
Note: result above is the meansigma methods of 3 experimental grouies
Result above shows, in silkworm breeding environment, the size of ozone concentration also growth and development state with silkworm has
The most corresponding indexical relation, when in breeding environment, ozone concentration is higher, the growth of silkworm is affected.
Specifically, in breeding environment, ozone concentration is as auxiliary characteristics, predicts and evaluates silkworm development situation
Standard be:
When in silkworm breeding environment, ozone concentration is when less than 0.10ppm, and the biological characteristics of silkworm is the highest；
When in silkworm breeding environment, ozone concentration is between 0.10～0.30ppm, the biological characteristics of silkworm is relatively
High；
When in silkworm breeding environment, ozone concentration is between 0.30～0.50ppm, the biological characteristics of silkworm is relatively
Low；
When in silkworm breeding environment, ozone concentration is between 0.50～0.60ppm, the biological characteristics of silkworm is more
Low.
When in silkworm breeding environment, ozone concentration, when more than 0.60ppm, shows that the breeding environment of silkworm is the most non-
The most severe, it is not suitable for very much the growth promoter of silkworm.
Therefore, it can the Testing index using the ozone concentration in silkworm breeding environment as auxiliary, with ammonia concentration
Index be used in combination, predict and evaluate silkworm cultivation situation quality, especially for growth rate, food
The biological characteristics of the growth and development state such as Sang Liang, Cocoon layer ratio.
Embodiment 3 detection adds the ammonia of silkworm and ozone concentration after food Different Kinds of Pathogens
1, material
(only explanation the inventive method uses polyvoltinism Silkworm varieties " Gao Bai × bright moon ", it is possible to use other to test
Room or the upper conventional Silkworm varieties used of production, including original seed and the first generation of hybrid etc.), the pebrine disease of purification
Cause of disease micropartical spore (N.b) suspension, silkworm cytoplasmic polyhedrosis virus (BmCPV) cause of disease polyhedral body is hanged
Supernatant liquid, bacillary septicaemia cause of disease, above pathogen by Agricultural University Of South China's bombycology laboratory through artificial Mulberry
(the only explanation specific embodiment of the invention uses leaf successive propagation, it is possible to use other laboratory related experiment to make
Described pathogen), GT-2000 composite gas analyser.
2, method
(1) randomly select and play silkworm four ages, and be respectively put into 6 plastic casing (28cm × 18 sealed
Cm × 10cm), 20 silkworms of every box.
When four moltings in age, adding food concentration respectively is 105N.b/mL, 105CPV/mL, septicaemia cause of disease,
Every kind of cause of disease adds food two box silkworms, and 3 boxes are placed under natural environmental condition raising, and 3 boxes are placed in incubator raising.
(2) every morning 9:00AM side opens plastic box cover, stretches into plastics with the probe of gas detector
In the middle of box, detect the gas change of each group of test silkworm respectively.
3, experimental result
(1) the gas detecting result adding food virus BmCPV silkworm is as shown in table 9.
Table 9 adds the gas detecting result (four ages of on January 1st, 2016 rise and freshen food) of food virus BmCPV silkworm
Result shows, along with adding the prolongation eating the CPV cause of disease time, CO, the SO in silkworm rearing subenvironment2、
CH4Gas be not changed in, be 0；And NH3And O3The trend that is then increased significantly of concentration, especially
Be add food CPV virus 10 days after, after silkworm has all infected Midgut of Silkworm, Bombyx Mori type grasserie CPV, ammonia NH3Dense
Degree has reached the highest, and difference reaches pole significant level；And O3Concentration also have obvious rising trend.
(2) the gas detecting result adding food N.b silkworm is as shown in table 10.
Table 10 adds the gas detecting result (four ages of on January 1st, 2016 rise and freshen food) of food N.b silkworm
Result shows: along with the prolongation NH of infection time after silkworm infection pebrine3The rate of rise of concentration
Inconspicuous, even there are slightly biased few phenomenon, NH during the most ill dead silkworm3Concentration is the most on the low side.And O3Dense
Degree also has corresponding variation tendency.
(3) the gas detecting result adding the food former silkworm of septicaemia is as shown in table 11.
Table 11 adds the gas detecting result (five ages of on January 9th, 2016 rise and freshen food) of the food former silkworm of septicaemia
Result shows: after the former infected silkworm of septicaemia, prolongation over time, the NH in feeding environment3
Concentration has and first reduces the trend gradually risen afterwards, but the silkworm infected with BmCPV has notable difference, BmCPV
Infecting silkworm and be up obvious especially, this may be relevant with the classification of disease kind, intestinal in BmCPV main infection, shadow
Ring the digestion of silkworm, and antibacterial has added food main infection blood, and there is no dead silkworm, therefore NH3Change inconspicuous.
Meanwhile, O3Concentration also have corresponding change.
In sum, as shown in Figure 1, the situation of change of ammonia concentration in box after adding food Different Kinds of Pathogens: add
It is the fastest that the silkworm NH3 concentration of food BmCPV virus increases, and concentration is the highest, adds food septicaemia sick
Silkworm NH3 concentration that is former and that add food Nb cause of disease increasess slowly, and adds food septicaemia cause of disease to the later stage
Silkworm NH3Concentration is than the silkworm NH adding food Nb cause of disease3Concentration is high.Therefore can be by detecting the dense of silkworm room ammonia
Degree predicts flacherie, and a situation arises.
And O3Concentration also have a change of fluctuating accordingly, therefore, can be by the concentration conduct of detection silkworm room ozone
Auxiliary characteristics, a situation arises to predict flacherie.
Claims (10)
1. ammonia concentration application in the evaluation system setting up silkworm rearing situation quality in breeding environment.
Apply the most according to claim 1, it is characterised in that be in breeding environment ammonia concentration in prediction and/or the application of fine or not aspect of passing judgment on Growth And Development of Silkworm situation.
Apply the most according to claim 2, it is characterised in that the quality of described Growth And Development of Silkworm situation refers to the growth rate of silkworm, food Mulberry amount, Cocoon layer ratio and/or the height of worm pupa vital rates.
Application the most according to claim 1 or claim 2, it is characterised in that in inverse relation between described ammonia concentration and silkworm biological characteristics.
Apply the most according to claim 4, it is characterised in that the standard of described prediction and/or judge is: when in silkworm breeding environment, ammonia concentration is when less than 3.00ppm, and the biological characteristics of silkworm is the highest；When in silkworm breeding environment, ammonia concentration is between 3.00～8.00ppm, the biological characteristics of silkworm is higher；When in silkworm breeding environment, ammonia concentration is between 8.00～15.00ppm, the biological characteristics of silkworm is relatively low；When in silkworm breeding environment, ammonia concentration is between 15.00～25.00ppm, the biological characteristics of silkworm is lower；When in silkworm breeding environment, ammonia concentration is more than 25.00ppm, the biological characteristics of silkworm is the lowest.
6. one kind monitors and the new technique of early warning silkworm rearing situation quality by measuring ammonia concentration in breeding environment, it is characterized in that, being the ammonia concentration in detection silkworm breeding environment, ammonia concentration from low to high has indicated respectively by the good silkworm rearing situation to difference.
New technique the most according to claim 6, it is characterized in that, it is in silkworm breeding environment, be equipped with ammonia detector or ammonia detection probe, utilize the ammonia concentration in ammonia detector or ammonia detection probe detection breeding environment, different alarm songs or information alert can be sent when ammonia detector detects the ammonia of variable concentrations.
New technique the most according to claim 7, it is characterised in that when the ammonia concentration that ammonia detector detects is less than 3.00ppm, ammonia detector can send Pyatyi alarm；
When the ammonia concentration that ammonia detector detects is 3.00～8.00ppm, ammonia detector can send level Four alarm；
When the ammonia concentration that ammonia detector detects is 8.00～15.00ppm, ammonia detector can send three grades of alarms；
When the ammonia concentration that ammonia detector detects is 15.00～25.00ppm, ammonia detector can send second-level alarm；
When the ammonia concentration that ammonia detector detects is more than 25.00ppm, ammonia detector can send primary alarm.
9. ammonia concentration and ozone concentration combination, the application in the evaluation system setting up silkworm rearing situation quality in ozone concentration, or silkworm breeding environment in silkworm breeding environment.
New technique the most according to claim 6, it is characterised in that ozone concentration is as auxiliary characteristics in silkworm breeding environment, indicates the quality of silkworm rearing situation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239525.XA CN105850901A (en) | 2016-04-18 | 2016-04-18 | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610239525.XA CN105850901A (en) | 2016-04-18 | 2016-04-18 | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105850901A true CN105850901A (en) | 2016-08-17 |
Family
ID=56632484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610239525.XA Pending CN105850901A (en) | 2016-04-18 | 2016-04-18 | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105850901A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109523029A (en) * | 2018-09-28 | 2019-03-26 | 清华大学深圳研究生院 | For the adaptive double from driving depth deterministic policy Gradient Reinforcement Learning method of training smart body |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1699394A1 (en) * | 1989-08-29 | 1991-12-23 | Украинская сельскохозяйственная академия | Method of oak silkworm growing |
| JPH0819372A (en) * | 1994-07-08 | 1996-01-23 | Shinki Syst:Kk | Production of silk protein |
| CN1539275A (en) * | 2003-10-24 | 2004-10-27 | 张家珊 | Method and equipment for rearing domestic silkworm and fullgrown silkworm |
| CN202814449U (en) * | 2012-04-27 | 2013-03-20 | 西南民族大学 | A multifunctional animal house environment monitor |
-
2016
- 2016-04-18 CN CN201610239525.XA patent/CN105850901A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1699394A1 (en) * | 1989-08-29 | 1991-12-23 | Украинская сельскохозяйственная академия | Method of oak silkworm growing |
| JPH0819372A (en) * | 1994-07-08 | 1996-01-23 | Shinki Syst:Kk | Production of silk protein |
| CN1539275A (en) * | 2003-10-24 | 2004-10-27 | 张家珊 | Method and equipment for rearing domestic silkworm and fullgrown silkworm |
| CN202814449U (en) * | 2012-04-27 | 2013-03-20 | 西南民族大学 | A multifunctional animal house environment monitor |
Non-Patent Citations (2)
| Title |
|---|
| 上田悟、吴载德 等: "养蚕环境（二）", 《蚕桑通报》 * |
| 王建芳 等: "臭氧消毒对家蚕病原体的灭火作用研究初报", 《经济动物学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109523029A (en) * | 2018-09-28 | 2019-03-26 | 清华大学深圳研究生院 | For the adaptive double from driving depth deterministic policy Gradient Reinforcement Learning method of training smart body |
| CN109523029B (en) * | 2018-09-28 | 2020-11-03 | 清华大学深圳研究生院 | Self-adaptive double-self-driven depth certainty strategy gradient reinforcement learning method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Alfaro et al. | The complex interactions of Ostreid herpesvirus 1, Vibrio bacteria, environment and host factors in mass mortality outbreaks of Crassostrea gigas | |
| Vijverberg | Culture techniques for studies on the growth, development and reproduction of copepods and cladocerans under laboratory and in situ conditions: a review | |
| Bailey et al. | Salmonella prevalence in free-range and certified organic chickens | |
| Bates et al. | Relationship of Campylobacter isolated from poultry and from darkling beetles in New Zealand | |
| Vogelweith et al. | Host plant variation plastically impacts different traits of the immune system of a phytophagous insect | |
| Hall et al. | Avian wildlife mortality events due to salmonellosis in the United States, 1985–2004 | |
| Moriarty et al. | Survival of Escherichia coli, Enterococci, and Campylobacter spp. in sheep feces on pastures | |
| Clegg et al. | Risk factors associated with increased mortality of farmed Pacific oysters in Ireland during 2011 | |
| Forberg et al. | A protocol and cultivation system for gnotobiotic Atlantic cod larvae (Gadus morhua L.) as a tool to study host microbe interactions | |
| Trudnowska et al. | Taxonomic and size structures of zooplankton communities in the fjords along the Atlantic water passage to the Arctic | |
| Mennerat et al. | Invest more and die faster: The life history of a parasite on intensive farms | |
| CN109423463A (en) | The pseudomonad strain Jdn1 and its microbial inoculum for preventing and treating root knot nematode disease | |
| Regaiolli et al. | Gum feeder as environmental enrichment for zoo marmosets and tamarins | |
| CN103513032B (en) | Chicken nose tracheae bird bacillus hemagglutination inhibition antibody detection kit, preparation method and application | |
| Wang et al. | Survival status of Penaeus vannamei is associated with the homeostasis and assembly process of the intestinal bacterial community | |
| CN105850901A (en) | Detection of concentration of ammonia in breeding environment and application thereof in establishing silkworm growth and development judgment system | |
| Pathirana et al. | Impact of seawater temperature on the Pacific oyster (Crassostrea gigas) microbiome and susceptibility to disease associated with Ostreid herpesvirus-1 (OsHV-1) | |
| Laub et al. | Epidemiology and antibiotic sensitivity of Staphylococcus aureus nasal carriage in children in Hungary | |
| CN203467403U (en) | Improved metabolism cage for poultries | |
| CN108548746A (en) | A method of measuring feather degradation rate | |
| Kuo et al. | Quantitative measurement of the immune response and sleep in Drosophila | |
| CN105519494B (en) | A kind of experiment supports seedling device and its test method with foster worm | |
| CN205720144U (en) | Gas concentration and the detecting system of physical signs in breeding environment | |
| CN205826005U (en) | The gas concentration of silkworm breeding environment and the comprehensive detection system of physical signs | |
| CN101936890B (en) | Method for quickly determining bacterial number in Riemerella anatipestifer culture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160817 |