CN105850266A - 一种有效保存水鳖种子的方法 - Google Patents

一种有效保存水鳖种子的方法 Download PDF

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CN105850266A
CN105850266A CN201610215541.5A CN201610215541A CN105850266A CN 105850266 A CN105850266 A CN 105850266A CN 201610215541 A CN201610215541 A CN 201610215541A CN 105850266 A CN105850266 A CN 105850266A
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container
seed
seeds
frogbit
hydrocharis
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CN105850266B (zh
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赵素婷
李伟
刘浩
张聪
秦真
郭曾莉
管波
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Wuhan De Yi Environmental Technology Co. Ltd.
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Wuhan Changbao Environment Protection Engineering Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01FPROCESSING OF HARVESTED PRODUCE; HAY OR STRAW PRESSES; DEVICES FOR STORING AGRICULTURAL OR HORTICULTURAL PRODUCE
    • A01F25/00Storing agricultural or horticultural produce; Hanging-up harvested fruit

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

本发明公开了一种有效保存水鳖种子的方法,其步骤是:A、每年10‑11月收集成熟的水鳖果实,收集来的果实放在一个盛水容器中,容器内加入自来水,自来水的体积为水鳖种子体积的1-3倍,让果实自然腐烂;B、次年2月‑3月,得到水鳖种子,用自来水反复3-8次冲洗干净后,把种子放在纱袋中;C、把盛有水鳖种子的纱袋放在容器中,容器大小为放有水鳖种子的纱袋的8-12倍左右,容器上覆满沙子;D、把放有沙子和种子的容器放在盛满水的水池,容器稳定放置于水下50‑100cm处保藏。方法简便易行,便于实现,保存成本低。由于种子具有高的遗传多样性,保藏所需空间小,成熟水鳖种子的活力在2年后仍然维持在90%以上。

Description

一种有效保存水鳖种子的方法
技术领域
本发明属于资源与环境技术领域的生物多样性保护技术范畴,更具体涉及一种有效保存水鳖(Hydrocharis dubia)种子的方法,适用于利用种子大规模保藏不同来源、具有丰富遗传多样性的水鳖种质资源。
背景技术
统计表明,全世界约有50%的植物物种受到灭绝的威胁,如不及时采取有效的保护措施,到21世纪末将有2/3的维管植物消失。迁地保护已经成为保护植物生物多样性的重要措施之一,其中建立以收集种子为主体的基因库是当今保护植物种质资源最为普遍、且可靠易行的方式,在世界范围内库存的约6100000份种质资源中,近90%是以种子形式保存的。种子保存是植物种质遗传多样性迁地保护的一种廉价而有效的方法,利用种子进行植物的迁地保护。
水生植物所处水域湿地是目前最受威胁的生境类型之一,各种日益频繁剧烈的人为活动所伴随的生境丧失、片段化、污染与富营养化等极大地威胁着水生植物的生存发育,同时全球变化导致的水文过程变化进一步改变了水生植物所处生境的水文情势,使得许多水生植物在很短的时期内种群规模极度衰减乃至濒危甚至消失,导致水生植物的灭绝速率远高于陆生植物,其濒危现状更为严重。对水生植物开展迁地保护已经迫在眉睫,这将对以后水生植物的引种回归、种群复壮及利用广布种对湖泊进行生态修复都具有重要意义。水生植物的有性繁殖虽然相对有限,但由于有性繁殖是新基因形成和新基因型产生的主要手段,同时,有性繁殖形成的种子还可能是沉水植物遭到极端干扰毁坏后种群恢复的基础,因此,种子在水生植物种质资源保存中具有不可替代的特殊地位,在生物进化中也起到了十分重要的作用。
发明内容
本发明的目的在于克服现有水生植物种子保存方法的缺陷和不足,是在于提供了一种有效保存水鳖种子活力的方法,方法简便易行,便于实现,保存成本低。由于种子具有高的遗传多样性,保藏所需空间小,管理简便,本发明可以在较小的空间里实现不同来源的、具高遗传多样性的水鳖种质资源的大规模保藏,同时成熟水鳖种子的活力在2年后仍然维持在90%以上(以萌发率计算)。
为了实现上述的目的,本发明采用以下技术措施:
一种有效保存水鳖种子的方法,其步骤是:
A、 每年10月-11月收集成熟的水鳖果实(果实颜色发白为成熟的标志),收集来的果实放在一个盛水容器中,容器的体积是水鳖种子体积的2-4倍,容器内加入自来水,自来水的体积为水鳖种子体积的1-3倍,让果实自然腐烂;
B、 次年2月-3月,得到大量的水鳖种子,用自来水反复(3-8次)冲洗干净后,把种子放在纱袋(孔径大小为0.5×05.mm)中;
C、 把盛有水鳖种子的纱袋放在小容器(无特殊要求,市售小塑料桶即可)中,容器大小为放有水鳖种子的纱袋的8-12倍左右,容器上覆满洗净的普通河沙;
D、把放有沙子和种子的容器放在盛满水的水池或其他水体底部,为方便管理与后续工作,建议容器稳定放置于水下50-100cm处保藏,保藏过程中避免扰动。该保藏条件下,水鳖种子活力可以较长时间维持,以萌发率计算的种子活力2年后仍然可以保持在90%以上,同时期采用其他方法保存的种子活力基本为零。
本发明与现有技术相比,具有以下优点和效果:
由于种子具有高的遗传多样性,保藏所需空间小,管理简便,本发明可以在较小的空间里实现不同来源的、具高遗传多样性的水鳖种质资源的大规模保藏,保存方法简便易行,便于实现,且保存成本低,利用本发明保存成熟的水鳖种子,2年后种子活力(以萌发率计算)仍然可保持在90%以上。
具体实施方式
实施例1:
一种有效保存水鳖种子的方法,其步骤是:
A、每年10月或11月的上中旬收集成熟的水鳖果实(果实颜色发白为成熟的标志),收集来的果实放在一个盛水容器中,容器的体积是水鳖种子体积的2或3或4倍,容器内加入自来水,自来水的体积为水鳖种子体积的1或2或3倍,让果实自然腐烂;
B、次年2月或3月的任何日期,得到大量的水鳖种子,用自来水反复3或4或5或6或7或8次)冲洗干净后,把种子放在纱袋(孔径大小为0.5×05.mm)中;
C、把盛有水鳖种子的纱袋放在小容器中,容器大小为放有水鳖种子的纱袋的8或9或10或11或12倍左右,容器上覆满洗净的普通河沙;
D、把放有沙子和种子的容器放在盛满水的水池或其他水体底部,为方便管理与后续工作,建议容器稳定放置于水下50或60或70或80或90或100cm处保藏,保藏过程中避免扰动。该保藏条件下,水鳖种子活力可以较长时间维持。

Claims (1)

1.一种有效保存水鳖种子的方法,其步骤是:
(1)每年10月-11月收集成熟的水鳖果实,收集来的果实放在一个盛水容器中,容器的体积是水鳖种子体积的2-4倍,容器内加入自来水,自来水的体积为水鳖种子体积的1-3倍,让果实自然腐烂;
(2)次年2月-3月,得到水鳖种子,用自来水反复3-8次冲洗干净后,把种子放在纱袋中;
(3)把盛有水鳖种子的纱袋放在容器中,容器大小为放有水鳖种子的纱袋的8-12倍,容器上覆满洗净的河沙;
(4)把放有沙子和种子的容器放在盛满水的水池,容器稳定放置于水下50-100cm处保藏。
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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101702967A (zh) * 2009-09-21 2010-05-12 上海市园林科学研究所 提高海菜花种子萌发率的种子冬季储存及春季栽培的方法
CN102498792A (zh) * 2011-10-20 2012-06-20 中国科学院武汉植物园 一种提高竹叶眼子菜种子萌发率的方法
US20150342132A1 (en) * 2009-05-07 2015-12-03 Aquatech Bioenergy LLC System and method of stimulating ethanol production and growth of aquatic plants

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150342132A1 (en) * 2009-05-07 2015-12-03 Aquatech Bioenergy LLC System and method of stimulating ethanol production and growth of aquatic plants
CN101702967A (zh) * 2009-09-21 2010-05-12 上海市园林科学研究所 提高海菜花种子萌发率的种子冬季储存及春季栽培的方法
CN102498792A (zh) * 2011-10-20 2012-06-20 中国科学院武汉植物园 一种提高竹叶眼子菜种子萌发率的方法

Non-Patent Citations (1)

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