CN105842215A - NAND logic gate based on BSA/3-MPA-Au nanoclusters and establishment method of NAND logic gate - Google Patents
NAND logic gate based on BSA/3-MPA-Au nanoclusters and establishment method of NAND logic gate Download PDFInfo
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Abstract
The invention discloses an NAND logic gate based on BSA/3-MPA-Au nanoclusters and an establishment method of the NAND logic gate .On the basis of the phenomenon that when Fe2+ and H2O2 coexist in an acetate buffer solution with the pH being 3.0, bovine serum albumin-3-thiohydracrylic acid-Au nanocluster fluorescence can be induced to be quenched, Fe2+ and H2O2 serve as input signals, the bovine serum albumin-3-thiohydracrylic acid-Au nanoclusters serve as a signal converter, and an NAND type logic gate system is established .The logic gate system has the advantages that operation is easy and convenient (no modification or marking process is needed), and signal reading is diverse, and good application prospects are achieved in the fields of clinical diagnosis, chemical sensing, environmental monitoring and the like .
Description
Technical field
The present invention relates to NAND gate based on BSA/3-MPA-gold nano cluster and construction method thereof, belong to field of nanometer technology.
Background technology
In the past few decades, chemistry boolean logic gate has caused the extensive concern of people, and it has been applied to the numerous areas such as clinical diagnosis, chemical sensitisation and environmental monitoring.At present, by using different materials (such as nucleic acid, enzyme, organic molecule and nano material) different classes of gate can be constructed, such as AND, OR, IMPLICATION, NAND, NOR and INHIBIT etc..But, most logical operation system has the disadvantage in that modification or the labeling process that (1) comprises complexity, and cost is high；(2) can not reset；(3) portable poor, it is difficult to be connected on the surface of solids；(4) integration between multiple input signal and each gate can not be processed extremely difficult simultaneously.
Fluorescence method has the outstanding advantages such as simple to operate, quick, highly sensitive, high specificity, is a kind of method of often selecting of logic gate device.Although a lot of fluorescent dyes have been in the news and can perform logical operations, but cost is high, light stability is poor, easily occur the problems such as autoxidation, toxicity are big to limit it applies further.In recent years, metal nanometer cluster, especially gold nano cluster, the fluorescent nano material novel as a class receives much concern.Compared with small molecule organic fluorescent dyestuff, the advantages such as gold nano cluster material has that photophysical property is good, specific surface area is big, toxicity is low, surface is prone to modify and photoluminescent property is adjustable.Therefore, exploitation gold nano cluster is significantly as realizing a kind of new material of gate operation.
The present invention is with Fe2+And H2O2As input signal, with bovine serum albumin-3-mercaptopropionic acid-gold nano cluster (that is: BSA/3-MPA-gold nano cluster) as signal adapter, construct a kind of NAND gate.NAND gate constructed by the present invention has the advantages such as easy and simple to handle, signal-obtaining is diversified.
Summary of the invention
It is an object of the invention to provide a kind of NAND gate based on BSA/3-MPA-fluorescent au nanocluster material and construction method thereof.
To achieve these goals, the present invention is by the following technical solutions: of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that input signal-1 is for Fe2+, input signal-2 is H2O2；Signal adapter is BSA/3-MPA-gold nano cluster；Output signal is the fluorescence of BSA/3-MPA-gold nano cluster.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that used BSA/3-MPA-gold nano cluster is prepared by following method: 2.5 mL concentration are the bovine serum albumin of 50 mg/mL and chlorauric acid solution mix homogeneously that 2.5 mL concentration are 10 mmol/L, and being subsequently adding 0.25 mL concentration is 1
Sodium hydroxide solution and the 0.25 mL concentration of mol/L are 4
The 3-mercaptopropionic acid of mol/L, shaking mixing, 1 h is reacted under 4 ° of C, reactant liquor is become colorless by light yellow, the reactant liquor bag filter of molecular cut off 7000 is dialysed 48 hours in 20 mmol/L pH=3.0 phosphate buffers, continue dialysis 12 hours the most in deionized water, obtain BSA/3-MPA-gold nano cluster solution.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that input signal-1 is for Fe2+Concentration be preferably 50 μm ol/L, input signal-2 is H2O2Concentration be preferably 20 μm ol/L.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that, when containing input signal, being defined as 1；When not containing input signal, it is defined as 0.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that four kinds of input signal forms are respectively as follows: and both do not contain 50 μm ol/L Fe2+Do not contain again 20 μm ol/L H2O2, it is defined as (0,0)；Containing 50 μm ol/L Fe2+But do not contain 20 μm ol/L
H2O2, it is defined as (1,0)；Do not contain 50 μm ol/L Fe2+But containing 20 μm ol/L H2O2, it is defined as (0,1)；Both 50 μm ol/L Fe had been contained2+Contain again 20 μm ol/L H2O2, it is defined as (1,1).
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that visualization under uviol lamp 302 nm wavelength, when BSA/3-MPA-gold nano cluster has orange-yellow fluorescence, output signal is defined as 1, and when BSA/3-MPA-gold nano cluster does not has orange-yellow fluorescence, output signal is defined as 0.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that when fluorescent spectrophotometer assay excitation wavelength is 320 nm 575
Fluorescence intensity at nm wavelength, when fluorescence intensity is more than 100, output signal is defined as 1；When fluorescence intensity is less than 100, output signal is defined as 0.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that output signal is 1 when input signal is (0,0)；When input signal is (1,0), output signal is 1；When input signal is (0,1), output signal is 1；When input signal is (1,1), output signal is 0.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate construction method, it is characterized in that being separately added into 475 in 25 μ L BSA/3-MPA-gold nano cluster solution
The μ L acetate buffer that pH is 3.0 containing varying input signal, mixing, room temperature reaction 10 minutes, under uviol lamp 302 nm wavelength, whether visualization BSA/3-MPA-gold nano cluster has orange-yellow fluorescence or is 320 by fluorescent spectrophotometer assay excitation wavelength
Fluorescence intensity at 575 nm wavelength during nm.
Of the present inventionBased on BSA/3-MPA- Gold nano cluster NAND Gate construction method, it is characterized in that input signal-1 is for Fe2+Concentration be preferably 50 μm ol/L, input signal-2 is H2O2Concentration be preferably 20 μm ol/L, when containing input signal, be defined as 1；When not containing input signal, being defined as 0, four kinds of input signal forms are respectively as follows: and both do not contain 50 μm ol/L Fe2+Do not contain again 20 μm ol/L H2O2, it is defined as (0,0)；Containing 50 μm ol/L Fe2+But do not contain 20 μm ol/L
H2O2, it is defined as (1,0)；Do not contain 50 μm ol/L Fe2+But containing 20 μm ol/L H2O2, it is defined as (0,1)；Both 50 μm ol/L Fe had been contained2+Contain again 20 μm ol/L H2O2, it is defined as (1,1)；Visualization under uviol lamp 302 nm wavelength, when BSA/3-MPA-gold nano cluster has orange-yellow fluorescence, output signal is defined as 1, when BSA/3-MPA-gold nano cluster does not has orange-yellow fluorescence, output signal is defined as 0, or fluorescent spectrophotometer assay excitation wavelength is 320
Fluorescence intensity at 575 nm wavelength during nm, when fluorescence intensity is more than 100, output signal is defined as 1；When fluorescence intensity is less than 100, output signal is defined as 0；When input signal is (0,0), output signal is 1；When input signal is (1,0), output signal is 1；When input signal is (0,1), output signal is 1；When input signal is (1,1), output signal is 0；The BSA/3-MPA-gold nano cluster used is prepared by following method: 2.5 mL concentration are the bovine serum albumin of 50 mg/mL and chlorauric acid solution mix homogeneously that 2.5 mL concentration are 10 mmol/L, and being subsequently adding 0.25 mL concentration is 1
Sodium hydroxide solution and the 0.25 mL concentration of mol/L are 4
The 3-mercaptopropionic acid of mol/L, shaking mixing, 1 h is reacted under 4 ° of C, reactant liquor is become colorless by light yellow, the reactant liquor bag filter of molecular cut off 7000 is dialysed 48 hours in 20 mmol/L pH=3.0 phosphate buffers, continue dialysis 12 hours the most in deionized water, obtain BSA/3-MPA-gold nano cluster solution.
Specifically, the present invention is by the following technical solutions:
(1) preparation of BSA/3-MPA-fluorescent au nanocluster material
The all glass drying ovens used in procedure below all soak through chloroazotic acid, and thoroughly clean with distilled water, dry.The preparation process of BSA/3-MPA-fluorescent au nanocluster material is as follows: 2.5 mL concentration are 50
Bovine serum albumin and the 2.5 mL concentration of mg/mL are 10
The chlorauric acid solution mix homogeneously of mmol/L, is subsequently adding 0.25
ML concentration is the sodium hydroxide solution and 0.25 of 1 mol/L
ML concentration is the 3-mercaptopropionic acid of 4 mol/L, shaking mixing, reacts 1 h under 4 ° of C, and reactant liquor is become colorless by light yellow.By reactant liquor with the bag filter of molecular cut off 7000 20
Mmol/L pH=3.0 phosphate buffer is dialysed 48 hours, continues dialysis 12 hours the most in deionized water, obtain BSA/3-MPA-gold nano cluster solution.
(2) structure of NOR-type gate
Take 4 EP pipes, respectively add 25
BSA/3-MPA-gold nano cluster solution prepared by μ L step (), is then separately added into the 475 μ L acetate buffer (pH=3.0) containing varying input signal, mixing, room temperature reaction 10 minutes, uviol lamp 302
Visualization or by the fluorescence intensity (excitation wavelength is 320 nm) at fluorescent spectrophotometer assay 575 nm wavelength under nm wavelength.The input signal-1 of NAND liquid phase gate is 50
μmol/L Fe2+, input signal-2 is 20
μmol/L H2O2。
Advantages of the present invention:
(1) present invention is based on Fe2+And H2O2A kind of NAND gate that can induce the fluorescence generation quencher of BSA/3-MPA-gold nano cluster when pH=3.0 acetate buffer solution coexists and design.
(2) signal adapter BSA/3-MPA-gold nano cluster preparation process used in the present invention is simple and quick.
(3) the gate fast response time constructed by the present invention, can complete signal output in 10 minutes.
(4) gate constructed by the present invention has the outstanding advantages such as easy and simple to handle, signal-obtaining is diversified.
Accompanying drawing explanation
Fig. 1 is gold nano cluster solution outside drawing under uviol lamp 302 nm wavelength after varying input signal effect.
Fig. 2 be after varying input signal effect fluorescence intensity figure at gold nano cluster solution 575 nm wavelength (excitation wavelength is 320
Nm).
Detailed description of the invention
The input signal-1 of NAND gate is 50 μm ol/L Fe2+, input signal-2 is 20 μm ol/L H2O2.When containing input signal, it is defined as 1；When not containing input signal, it is defined as 0.Four kinds of input signal forms are respectively as follows: and both do not contain 50 μm ol/L Fe2+Do not contain again 20 μm ol/L H2O2, it is defined as (0,0)；Containing 50 μm ol/L Fe2+But do not contain 20 μm ol/L
H2O2, it is defined as (1,0)；Do not contain 50 μm ol/L Fe2+But containing 20 μm ol/L H2O2, it is defined as (0,1)；Both 50 μm ol/L Fe had been contained2+Contain again 20 μm ol/L H2O2, it is defined as (1,1).Output signal is the fluorescence of BSA/3-MPA-gold nano cluster.When BSA/3-MPA-gold nano cluster has fluorescence, it is defined as 1；When BSA/3-MPA-gold nano cluster unstressed configuration, it is defined as 0.Output signal is divided into two kinds of reading forms: visualization under (1) uviol lamp 302 nm wavelength, has orange-yellow fluorescence and is defined as 1, does not have orange-yellow fluorescence and be defined as 0；(2) by the fluorescence intensity at fluorescent spectrophotometer assay 575 nm wavelength, (excitation wavelength is 320
Nm), fluorescence intensity is defined as 1 more than 100, and fluorescence intensity is defined as 0 less than 100.
Embodiment 1:
The preparation of BSA/3-MPA-fluorescent au nanocluster material: 2.5
ML concentration is the bovine serum albumin and 2.5 of 50 mg/mL
ML concentration is the chlorauric acid solution mix homogeneously of 10 mmol/L, and being subsequently adding 0.25 mL concentration is 1
Sodium hydroxide solution and the 0.25 mL concentration of mol/L are 4
The 3-mercaptopropionic acid of mol/L, shaking mixing, react 1 hour under 4 ° of C, reactant liquor is become colorless by light yellow.The reactant liquor bag filter of molecular cut off 7000 is dialysed 48 hours in 20 mmol/L pH 3 phosphate buffers, continues dialysis 12 hours the most in deionized water, obtain BSA/3-MPA-gold nano cluster solution.4 ° of C dark places preserve, and can keep the most stable of at least two moon.
Embodiment 2:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
The acetate buffer solution of μ L 50 mmol/L pH=3.0, room temperature reaction 10 minutes, to observe under uviol lamp 302 nm wavelength, solution has orange-yellow fluorescence.I.e. when input signal is (0,0), output signal is that 1(is shown in Fig. 1).
Embodiment 3:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
The acetate buffer solution of μ L 50 mmol/L pH=3.0, room temperature reaction 10 minutes, (excitation wavelength is 320 to the fluorescence intensity at fluorescent spectrophotometer assay 575 nm wavelength
Nm), fluorescence intensity is more than 100.I.e. when input signal is (0,0), output signal is that 1(is shown in Fig. 2).
Embodiment 4:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 50 μm ol/L
Fe2+The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, observe under uviol lamp 302 nm wavelength, solution has orange-yellow fluorescence.I.e. when input signal is (1,0), output signal is that 1(is shown in Fig. 1).
Embodiment 5:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 50 μm ol/L
Fe2+The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, the fluorescence intensity (excitation wavelength is 320 nm) at fluorescent spectrophotometer assay 575 nm wavelength, fluorescence intensity is more than 100.I.e. when input signal is (1,0), output signal is that 1(is shown in Fig. 2).
Embodiment 6:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 20 μm ol/L
H2O2The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, observe under uviol lamp 302 nm wavelength, solution has orange-yellow fluorescence.I.e. when input signal is (0,1), output signal is that 1(is shown in Fig. 1).
Embodiment 7:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 20 μm ol/L
H2O2The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, the fluorescence intensity (excitation wavelength is 320 nm) at fluorescent spectrophotometer assay 575 nm wavelength, fluorescence intensity is more than 100.I.e. when input signal is (0,1), output signal is that 1(is shown in Fig. 2).
Embodiment 8:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 50 μm ol/L
Fe2+With 20 μm ol/L H2O2The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, observe under uviol lamp 302 nm wavelength, solution does not have orange-yellow fluorescence.I.e. when input signal is (1,1), output signal is that 0(is shown in Fig. 1).
Embodiment 9:
475 are added in the BSA/3-MPA-gold nano cluster solution that 25 μ L embodiments 1 prepare
μ L contains 50 μm ol/L
Fe2+With 20 μm ol/L H2O2The acetate buffer solution of 50 mmol/L pH=3.0, room temperature reaction 10 minutes, (excitation wavelength is 320 to the fluorescence intensity at fluorescent spectrophotometer assay 575 nm wavelength
Nm), fluorescence intensity is less than 100.I.e. when input signal is (1,1), output signal is that 0(is shown in Fig. 2).
The foregoing is only the exemplary embodiments of the present invention, not in order to limit the present invention, all any amendments made within the spirit and principles in the present invention, equivalent and improvement etc., should be included within the scope of the present invention.
Claims (10)
1.A kind of Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that input signal-1 is for Fe2+, input signal-2 is H2O2；Signal adapter is BSA/3-MPA-gold nano cluster；Output signal is the fluorescence of BSA/3-MPA-gold nano cluster.
The most according to claim 1Based on BSA/3-MPA- Gold nano cluster NAND Gatenull，It is characterized in that used BSA/3-MPA-gold nano cluster is prepared by following method: 2.5 mL concentration are the bovine serum albumin of 50 mg/mL and chlorauric acid solution mix homogeneously that 2.5 mL concentration are 10 mmol/L，It is subsequently adding the sodium hydroxide solution that 0.25 mL concentration is 1 mol/L and the 3-mercaptopropionic acid that 0.25 mL concentration is 4 mol/L，Shaking mixing，1 h is reacted under 4 ° of C，Reactant liquor is become colorless by light yellow，The reactant liquor bag filter of molecular cut off 7000 is dialysed 48 hours in 20 mmol/L pH=3.0 phosphate buffers，Continue dialysis 12 hours the most in deionized water，Obtain BSA/3-MPA-gold nano cluster solution.
The most according to claim 1 and 2Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that input signal-1 is for Fe2+Concentration be 50 μm ol/L, input signal-2 is H2O2Concentration be 20 μm ol/L
。
The most according to claim 1 and 2Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that, when containing input signal, being defined as 1；When not containing input signal, it is defined as 0.
The most according to claim 3Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that four kinds of input signal forms are respectively as follows: and both do not contain 50 μm ol/L Fe2+Do not contain again 20 μm ol/L
H2O2, it is defined as (0,0)；Containing 50 μm ol/L
Fe2+But do not contain 20 μm ol/L H2O2, it is defined as (1,0)；Do not contain 50 μm ol/L Fe2+But containing 20 μm ol/L H2O2, it is defined as (0,1)；Both 50 μm ol/L Fe had been contained2+Contain again 20 μm ol/L H2O2, it is defined as (1,1).
The most according to claim 5Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that visualization under uviol lamp 302 nm wavelength, when BSA/3-MPA-gold nano cluster has orange-yellow fluorescence, output signal is defined as 1, and when BSA/3-MPA-gold nano cluster does not has orange-yellow fluorescence, output signal is defined as 0.
The most according to claim 5Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that the fluorescence intensity at 575 nm wavelength when fluorescent spectrophotometer assay excitation wavelength is 320 nm, when fluorescence intensity is more than 100, output signal is defined as 1；When fluorescence intensity is less than 100, output signal is defined as 0.
8. according to described in claim 6 or 7Based on BSA/3-MPA- Gold nano cluster NAND Gate, it is characterized in that output signal is 1 when input signal is (0,0)；When input signal is (1,0), output signal is 1；When input signal is (0,1), output signal is 1；When input signal is (1,1), output signal is 0.
9.A kind of Based on BSA/3-MPA- Gold nano cluster NAND Gate construction methodIt is characterized in that the acetate buffer that pH is 3.0 being separately added into 475 μ L in 25 μ L BSA/3-MPA-gold nano cluster solution containing varying input signal, mixing, room temperature reaction 10 minutes, fluorescence intensity at 575 nm wavelength when whether visualization BSA/3-MPA-gold nano cluster has orange-yellow fluorescence or be 320 nm by fluorescent spectrophotometer assay excitation wavelength under uviol lamp 302 nm wavelength.
The most according to claim 9Based on BSA/3-MPA- Gold nano cluster NAND Gate construction method, it is characterized in that input signal-1 is for Fe2+Concentration be preferably 50 μm ol/L, input signal-2 is H2O2Concentration be preferably 20 μm ol/L, when containing input signal, be defined as 1；When not containing input signal, being defined as 0, four kinds of input signal forms are respectively as follows: and both do not contain 50 μm ol/L Fe2+Do not contain again 20 μm ol/L H2O2, it is defined as (0,0)；Containing 50 μm ol/L Fe2+But do not contain 20 μm ol/L H2O2, it is defined as (1,0)；Do not contain 50 μm ol/L Fe2+But containing 20 μm ol/L H2O2, it is defined as (0,1)；Both 50 μm ol/L Fe had been contained2+Contain again 20 μm ol/L H2O2, it is defined as (1,1)；Visualization under uviol lamp 302 nm wavelength, when BSA/3-MPA-gold nano cluster has orange-yellow fluorescence, output signal is defined as 1, when BSA/3-MPA-gold nano cluster does not has orange-yellow fluorescence, output signal is defined as 0, or fluorescent spectrophotometer assay excitation wavelength fluorescence intensity at 575 nm wavelength when being 320 nm, when fluorescence intensity is more than 100, output signal is defined as 1；When fluorescence intensity is less than 100, output signal is defined as 0；When input signal is (0,0), output signal is 1；When input signal is (1,0), output signal is 1；When input signal is (0,1), output signal is 1；When input signal is (1,1), output signal is 0；The BSA/3-MPA-gold nano cluster used is prepared by following method: 2.5 mL concentration are the bovine serum albumin of 50 mg/mL and chlorauric acid solution mix homogeneously that 2.5 mL concentration are 10 mmol/L, it is subsequently adding the sodium hydroxide solution that 0.25 mL concentration is 1 mol/L and the 3-mercaptopropionic acid that 0.25 mL concentration is 4 mol/L, shaking mixing, 1 h is reacted under 4 ° of C, reactant liquor is become colorless by light yellow, the reactant liquor bag filter of molecular cut off 7000 is dialysed 48 hours in 20 mmol/L pH=3.0 phosphate buffers, continue dialysis 12 hours the most in deionized water, obtain BSA/3-MPA-gold nano cluster solution.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103364352A (en) * | 2013-07-19 | 2013-10-23 | 福建医科大学 | Method for rapid determination of sulphur ions with nanogold as logic gate color developing probe |
| CN104330391A (en) * | 2014-11-04 | 2015-02-04 | 福建医科大学 | Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters |
| US20150174657A1 (en) * | 2013-02-19 | 2015-06-25 | Council Of Scientific And Industrial Research | Nano aggregates of molecular ultra small clusters of noble metals and a process for the preparation thereof |
| CN105067601A (en) * | 2015-07-16 | 2015-11-18 | 重庆大学 | Functional test paper film based on nanogold and method for detecting iron ions |
| CN105120849A (en) * | 2013-03-14 | 2015-12-02 | Ran生物技术公司 | Methods and materials for detection of biologicals |
| CN105199716A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 3-mercaptopropoinate-bovine serum albumin-gold nanoclusters and preparation method thereof |
-
2016
- 2016-05-02 CN CN201610285001.4A patent/CN105842215A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150174657A1 (en) * | 2013-02-19 | 2015-06-25 | Council Of Scientific And Industrial Research | Nano aggregates of molecular ultra small clusters of noble metals and a process for the preparation thereof |
| CN105120849A (en) * | 2013-03-14 | 2015-12-02 | Ran生物技术公司 | Methods and materials for detection of biologicals |
| CN103364352A (en) * | 2013-07-19 | 2013-10-23 | 福建医科大学 | Method for rapid determination of sulphur ions with nanogold as logic gate color developing probe |
| CN104330391A (en) * | 2014-11-04 | 2015-02-04 | 福建医科大学 | Hydrogen peroxide measurement method based on N-acetyl-L-cysteine-gold nanoclusters |
| CN105067601A (en) * | 2015-07-16 | 2015-11-18 | 重庆大学 | Functional test paper film based on nanogold and method for detecting iron ions |
| CN105199716A (en) * | 2015-09-26 | 2015-12-30 | 福建医科大学 | 3-mercaptopropoinate-bovine serum albumin-gold nanoclusters and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| （英）席瓦尔著.: "《分子逻辑计算[M]》", 30 October 2014, 上海：华东理工大学出版社 * |
| FAN XIA,ETAL: "《Label-Free, Dual-Analyte Electrochemical Biosensors: A New Class of Molecular-Electronic Logic Gates》", 《J.AM.CHEM.SOC》 * |
| 马哈里克等著: "《12.3荧光分子逻辑电路》", 《微制造与纳米技术》 * |
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