CN103364352A - Method for rapid determination of sulphur ions with nanogold as logic gate color developing probe - Google Patents
Method for rapid determination of sulphur ions with nanogold as logic gate color developing probe Download PDFInfo
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Abstract
The invention discloses a method for rapid determination of sulphur ions with nanogold as a logic gate color developing probe. As thiourea can enable aggregation of a nanogold solution, the solution changes from wine red to blue, and sulphur ions can specifically inhibit thiourea from aggregating nanogold, variation of the solution color and ultraviolet absorption spectrum characteristics can be shown, so that the sulphur ion concentration can be determined. Thiourea can make the nanogold solution undergo aggregation and change the solution color from wine red to blue, while sulphur ions can specifically inhibit thiourea from aggregating nanogold, thus showing variation of the solution color and ultraviolet absorption spectrum characteristics. The limit of detection of visual observation is 4 micromol/L. The linear range of absorbance ratio determination is 2-9 micromol/L. After simple pretreatment, mineral water, tap water, river water, white grape wine, human urine and other actual samples can be subjected to sulphur ion content determination by the method.
Description
Technical field
The present invention relates to the quick content assaying method of the sulphion of colour developing probe take nm of gold as logic gate, belong to analytical chemistry and field of nanometer technology.
Background technology
Sulfide is common industrial chemicals and the chemical pollutants of industry such as papermaking, petrochemical industry, leather, and the Aquatic Ecosystem 2 human health of unifying is all produced harm.Under acid condition, sulfide changes sulfuretted hydrogen into, can endanger cytochrome, oxidase, causes the cell tissue anoxic, even threat to life.Sulfuretted hydrogen is the corroding metal e-quipment and pipe also, and can be become sulfuric acid by microbiological oxidation, aggravation corrosivity.Sulfuretted hydrogen is described as the third endogenous gaseous signal molecule after nitrogen monoxide and carbon monoxide simultaneously, is a kind of powerful antioxidant and vascular relaxing factor.Studies show that in a large number, hydrogen sulfide levels can change in some diseases (such as Alzheimer's and Down syndrome).At present, the detection method of sulfide mainly comprises galvanochemistry, chromatogram etc.Yet these methods are owing to needing complicated sample pretreatment process, and are relatively time-consuming.In addition, these analytical technologies need expensive and advanced detecting instrument, and by the personnel operation that was subjected to professional training.Therefore, set up a kind of new method that can be widely used in Food Chemistry, biology and ecosystem medium sulphide content mensuration and still have very important practical significance.
In recent years, the molecule boolean logic gate has been subjected to increasing concern.Some research work show, the enforcement of molecule boolean logic gate can improve the performance of method, and promote the progress in chemical sensitisation, medical diagnosis and functional biochemistry field.Based on the foundation of the logic gate color development system of golden nanometer particle high extinction coefficient and Range-dependent optical characteristics, because of its have high sensitivity, fast, low-cost and can for advantages such as naked eyes identifications, become one of current research focus.At present, use DNAzyme method, aptamer method, part coordination method etc., set up many nm of gold logic gate color development system.But the input signal of most of logic gate systems of above report is (biology) molecule and metallic ion, and is extremely rare as the report of logic gate input signal with negative ion.
The present invention with thiocarbamide and sulphion as input signal, take the nm of gold of sodium citrate reducing process preparation as the colour developing probe as signal converter, made up a kind of " inhibition " (INHIBIT) logic gate of behavior that has, namely " suppress door ", and successfully be applied to micro-detection of sulfion.
Summary of the invention
The objective of the invention is nm of gold take sodium citrate reducing process preparation as logic gate colour developing probe, a kind of quick, easy, sensitive sulphion new detecting method is provided.
To achieve these goals, the present invention is by the following technical solutions:
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that based on thiocarbamide nano-Au solution is produced assembles, solution becomes blueness by claret, and sulphion can suppress thiocarbamide specifically to the congregation of nm of gold, thus show the variation of solution colour and ultra-violet absorption spectrum feature, come
Measure sulphion concentration
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that utilizing visualization solution colour feature with the concentration of judgement sulphion or utilize absorbance ratio A
680/ A
520To judge the concentration of sulphion.
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that employed nm of gold adopts the method preparation of sodium citrate reduction gold chloride, the steps include: the chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliters of 0.1g/L of rear rapid adding, reaction solution is from the light yellow claret that becomes, after continuing to reflux 15 minutes, reaction solution slowly is cooled to room temperature and forms nm of gold.
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that 1:1 by volume with nano-Au solution and the concentration that contains the variable concentrations sulphion be 0.01 mol/L, contain the thiocarbamide of 5 ~ 7 μ mol/L, the phosphate buffered solution of pH=5.0 ~ 10.0 is mixed, 30 ℃ were reacted 3 ~ 5 minutes, visualization color characteristic or mensuration absorbance ratio A
680/ A
520, along with the increase of sulphion concentration, the color of nm of gold becomes blueness-purple-aubergine-redness by redness gradually when the visualization color characteristic, and the detection of visualization is limited to 4 μ mol/L; When measuring absorbance ratio A
680/ A
520The time, along with the increase absorbance ratio A of sulphion concentration
680/ A
520Reduce gradually A in 2 ~ 9 μ mol/L scopes
680/ A
520Linear with sulphion concentration.
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that employed nm of gold particle diameter is 13 nm, concentration is 3.1 nmol/L.
Described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that 1:1 by volume with nano-Au solution and the concentration that contains the variable concentrations sulphion be 0.01 mol/L, contain the thiocarbamide of 5 μ mol/L, the phosphate buffered solution of pH=9.0 is mixed, 30 ℃ were reacted 3 minutes, visualization color characteristic or mensuration absorbance ratio A
680/ A
520To judge the concentration of sulphion; Nano-Au solution is respectively 0.2 milliliter and 0.2 milliliter with the phosphate buffered solution volume that contains the variable concentrations sulphion.
Of the present invention
The rapid assay methods of sulphion in colour developing probe assay mineral water or tap water sample or the river sample take nm of gold as logic gate, comprise the steps: to get mineral water sample or tap water sample or river sample, at first use 0.22 micron membrane filtration, then in filtrate, add EDTA to final concentration be 0.1 mmol/L; Filtrate is regulated its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and make sample solution; 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
Of the present invention
The rapid assay methods of sulphion in the colour developing probe assay white wine take nm of gold as logic gate, comprise the steps: the white wine sample add EDTA to final concentration be 0.1 mmol/L, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and form sample solution; 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
Of the present invention
The rapid assay methods of sulphion in the colour developing probe assay human urine take nm of gold as logic gateComprise the steps: the human urine sample add EDTA to final concentration be 0.1 mmol/L, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and form sample solution, 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
Employed nm of gold adopts the method preparation of sodium citrate reduction gold chloride, the chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliter of 0.1 g/L of rear rapid adding, reaction solution is from the light yellow claret that becomes, after continuing to reflux 15 minutes, reaction solution slowly is cooled to room temperature and forms nm of gold.
Specifically, the technical solution used in the present invention is:
(1) preparation of nm of gold
All glasswares that use in the following process all soak through chloroazotic acid, and thoroughly clean with distilled water, dry.The preparation of nm of gold: the chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliter of 0.1 g/L of rear rapid adding, reaction solution after continuing to reflux 15 minutes, slowly is cooled to room temperature with reaction solution by from the light yellow claret that becomes.The nm of gold particle diameter of gained is 13 nm, and concentration is about 3.1 nmol/L, 4 ℃ of preservations.
(2) detection of sulfion
(phosphate buffered solution concentration is 0.01 mol/L 0.2 milliliter contains the phosphate buffered solution of the thiocarbamide of 5 μ mol/L, pH=9) nano-Au solution that adds 0.2 milliliter of step () preparation in, after mixing in 30 ℃ of reactions 3 minutes, the variation of visualization color or measure 680 nm and the absorbance ratio (A at 520 nm wavelength places
680/ A
520).Carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve.The detection of visualization is limited to 4 μ mol/L.
Advantage of the present invention:
(1) the present invention is based on thiocarbamide can make nano-Au solution produce gathering, solution becomes blueness by claret, and the specific inhibition thiocarbamide of sulphion energy is to the congregation of nm of gold, thereby show the variation of solution colour and ultra-violet absorption spectrum feature, can be directly used in the content detection of sulphion.
(2) nm of gold used in the present invention is directly obtained by sodium citrate reduction gold chloride, need not further to modify the preparation process Simple fast.
(3) the present invention is low to the processing requirements of sample, and anti-interference is good, can measure after only need adding ethylenediamine tetraacetic acid in the sample.
(4) detection speed of the present invention is fast, can finish pre-service and the detection of actual sample in 3 minutes.
(5) detection sensitivity of the present invention is high, and the detection by the visual inspection change color is limited to 4 μ mol/L.
Description of drawings
Fig. 1 is nm of gold change color figure (A) and corresponding absorbance ratio (A after the varying input signal effect under the system pH=9 condition of the present invention
680/ A
520) variation diagram (B).(0,0) expression does not contain thiocarbamide and sulphion among the figure; (0,1) expression sulfur-containing anion does not contain thiocarbamide; (1,0) expression contains thiocarbamide, not sulfur-containing anion; (1,1) expression contains thiocarbamide and sulphion.Wherein thiourea concentration is 5 μ mol/L, and sulphion concentration is 9 μ mol/L; The A figure color from left to right of Fig. 1 is red, redness, blueness, red (it is 0 that redness represents output signal, and it is 1 that blueness represents output signal).
Fig. 2 is the absorbance ratio figure after nm of gold and the sulphion effect under the different system pH conditions.Wherein thiourea concentration is 5 μ mol/L.
Fig. 3 is the absorbance ratio figure after nm of gold and the sulphion effect under the variable concentrations thiocarbamide condition.Wherein system pH is 9.
Fig. 4 is that system pH is 9, and thiourea concentration is that the absorbance ratio after nm of gold and the effect of variable concentrations sulphion is schemed over time under the 5 μ mol/L conditions.
Fig. 5 is that system pH is 9, and thiourea concentration is the change color figure after nm of gold and the effect of variable concentrations sulphion under the 5 μ mol/L conditions.Concrete change color from left to right is shown as, and when sulfur-containing anion not, solution is aobvious blue; When sulphion concentration was 4 μ mol/L, solution showed purple; When sulphion concentration is 7 μ mol/L, the solution displaing amaranth; When sulphion concentration was 9 μ mol/L, solution was aobvious red.
Fig. 6 is that system pH is 9, and thiourea concentration is the absorbance ratio (A after nm of gold and the effect of variable concentrations sulphion under the 5 μ mol/L conditions
680/ A
520) variation diagram.
Fig. 7 is that constructed logic gate system is applied to detection of sulfion figure in the actual sample.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.Among the figure not mark-on sample detection result be blueness, the mark-on sample is redness.
Embodiment
The present invention is described in detail below in conjunction with drawings and Examples:
All glasswares that use in following examples of the present invention all soak through chloroazotic acid, and thoroughly clean with distilled water, dry.
The preparation of embodiment 1 nm of gold:
The chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliter of 0.1 g/L of rear rapid adding, reaction solution after continuing to reflux 15 minutes, slowly is cooled to room temperature with reaction solution from the light yellow claret that becomes.The nm of gold particle diameter of gained is 13 nm, and concentration is about 3.1 nmol/L, 4 ℃ of preservations.
Embodiment 2:
Add 0.2 milliliter of phosphate buffered solution (phosphate buffered solution concentration is 0.01 mol/L, pH=9) that contains the thiocarbamide of 5 μ mol/L in the nm of gold that 0.2 milliliter of embodiment 1 makes, 30 ℃ were reacted 3 minutes.When thiocarbamide and sulphion all do not contain, or the sulf onyl ion, and thiocarbamide and sulphion are when all containing, namely (0,0), under (0,1), (1,1) three kinds of state, sedimentation does not occur in nm of gold, and solution colour keeps red (representing output signal is 0), absorbance ratio A
680/ A
520Little; When the sulf onyl urea, namely under (1,0) state, nm of gold generation sedimentation, solution colour becomes blue (represent output signal and be 1), absorbance ratio A
680/ A
520Large (see figure 1).
Embodiment 3:
Add 0.2 milliliter of phosphate buffered solution (phosphate buffered solution concentration is 0.01 mol/L, pH=5 ~ 10) that contains the thiocarbamide of 5 μ mol/L in the nm of gold that 0.2 milliliter of embodiment 1 makes, 30 ℃ were reacted 3 minutes, measured absorbance ratio A
680/ A
520As shown in Figure 2, Δ A
680/ A
520Be to reach maximum at 9 o'clock at system pH.
Embodiment 4:
Add 0.2 milliliter of phosphate buffered solution (phosphate buffered solution concentration is 0.01 mol/L, pH=9) that contains the thiocarbamide of 0 ~ 7 μ mol/L in the nm of gold that 0.2 milliliter of embodiment 1 makes, 30 ℃ were reacted 3 minutes, measured absorbance ratio A
680/ A
520As shown in Figure 3, Δ A
680/ A
520When being 5 μ mol/L, thiourea concentration reaches maximum.
Embodiment 5:
Add 0.2 milliliter of phosphate buffered solution (phosphate buffered solution concentration is 0.01 mol/L, pH=9) that contains the thiocarbamide of 5 μ mol/L in the nm of gold that 0.25 milliliter of embodiment 1 makes, 30 ℃ were reacted 0 ~ 5 minute, measured absorbance ratio A
680/ A
520As shown in Figure 4, absorbance ratio A
680/ A
520After 3 minutes, reach maximum.
Embodiment 6:
(phosphate buffered solution concentration is 0.01 mol/L to add 0.2 milliliter of phosphate buffered solution that contains the thiocarbamide of 5 μ mol/L in the nm of gold that 0.2 milliliter of embodiment 1 makes, pH=9), 30 ℃ were reacted 3 minutes, the variation of visualization color, and the result is as shown in Figure 5.When sulfur-containing anion not, solution is aobvious blue; When sulphion concentration was 4 μ mol/L, solution showed purple; When sulphion concentration is 7 μ mol/L, the solution displaing amaranth; When sulphion concentration was 9 μ mol/L, solution was aobvious red.
Embodiment 7:
Add 0.2 milliliter of phosphate buffered solution (phosphate buffered solution concentration is 0.01 mol/L, pH=9) that contains the thiocarbamide of 5 μ mol/L in the nm of gold that 0.2 milliliter of embodiment 1 makes, 30 ℃ were reacted 3 minutes, measured absorbance ratio A
680/ A
520As shown in Figure 6, absorbance ratio A
680/ A
520Increase with sulphion concentration reduces, A in 2 ~ 9 μ mol/L scopes
680/ A
520Linear with sulphion concentration.
Embodiment 8:
Mineral water is at first used 0.22 micron membrane filtration, and then adding the EDTA(final concentration is 0.1 mmol/L) to eliminate the interference of metallic ion.Filtrate is regulated its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.In the nm of gold that 0.2 milliliter of embodiment 1 makes, add respectively 0.2 milliliter of above-mentioned mark-on sample solution and mark-on sample solution not, 30 ℃ of reactions 3 minutes, the variation of visualization color or measure absorbance ratio A
680/ A
520(see figure 7).
Embodiment 9:
Tap water is at first used 0.22 micron membrane filtration, and then adding the EDTA(final concentration is 0.1 mmol/L) to eliminate the interference of metallic ion.Filtrate is regulated its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.In the nm of gold that 0.2 milliliter of embodiment 1 makes, add respectively 0.2 milliliter of above-mentioned mark-on sample solution and mark-on sample solution not, 30 ℃ of reactions 3 minutes, the variation of visualization color or measure absorbance ratio A
680/ A
520(see figure 7).
Embodiment 10:
River is at first used 0.22 micron membrane filtration, and then adding the EDTA(final concentration is 0.1 mmol/L) to eliminate the interference of metallic ion.Filtrate is regulated its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.In the nm of gold that 0.2 milliliter of embodiment 1 makes, add respectively 0.2 milliliter of above-mentioned mark-on sample solution and mark-on sample solution not, 30 ℃ of reactions 3 minutes, the variation of visualization color or measure absorbance ratio A
680/ A
520(see figure 7).
Embodiment 11:
It is 0.1 mmol/L that white wine adds the EDTA(final concentration) to eliminate the interference of metallic ion, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.In the nm of gold that 0.2 milliliter of embodiment 1 makes, add respectively 0.2 milliliter of above-mentioned mark-on sample solution and mark-on sample solution not, 30 ℃ of reactions 3 minutes, the variation of visualization color or measure absorbance ratio A
680/ A
520(see figure 7).
Embodiment 12:
It is 0.1 mmol/L that human urine adds the EDTA(final concentration) to eliminate the interference of metallic ion, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L.The sample that has before the sample preparation and do not add 10 μ mol/L sulphions is defined as respectively mark-on sample and mark-on sample not.In the nm of gold that 0.2 milliliter of embodiment 1 makes, add respectively 0.2 milliliter of above-mentioned mark-on sample solution and mark-on sample solution not, 30 ℃ of reactions 3 minutes, the variation of visualization color or measure absorbance ratio A
680/ A
520(see figure 7).
The above only is preferred embodiment of the present invention, and in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention are not equal to replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. one kind
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that based on thiocarbamide nano-Au solution is produced assembles, solution becomes blueness by claret, and sulphion can suppress thiocarbamide specifically to the congregation of nm of gold, thus show the variation of solution colour and ultra-violet absorption spectrum feature, come
Measure sulphion concentration
2. according to claim 1
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that utilizing visualization solution colour feature with the concentration of judgement sulphion or utilize absorbance ratio A
680/ A
520To judge the concentration of sulphion.
3. according to claim 1
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that employed nm of gold adopts the method preparation of sodium citrate reduction gold chloride, the steps include: the chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliters of 0.1g/L of rear rapid adding, reaction solution is from the light yellow claret that becomes, after continuing to reflux 15 minutes, reaction solution slowly is cooled to room temperature and forms nm of gold.
4. according to claim 1 or 3 described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that 1:1 by volume with nano-Au solution and the concentration that contains the variable concentrations sulphion be 0.01 mol/L, contain the thiocarbamide of 5 ~ 7 μ mol/L, the phosphate buffered solution of pH=5.0 ~ 10.0 is mixed, 30 ℃ were reacted 3 ~ 5 minutes, visualization color characteristic or mensuration absorbance ratio A
680/ A
520, along with the increase of sulphion concentration, the color of nm of gold becomes blueness-purple-aubergine-redness by redness gradually when the visualization color characteristic, and the detection of visualization is limited to 4 μ mol/L; When measuring absorbance ratio A
680/ A
520The time, along with the increase absorbance ratio A of sulphion concentration
680/ A
520Reduce gradually A in 2 ~ 9 μ mol/L scopes
680/ A
520Linear with sulphion concentration.
5. according to claim 3
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gate, it is characterized in that employed nm of gold particle diameter is 13 nm, concentration is 3.1 nmol/L.
6. according to claim 4
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that 1:1 by volume with nano-Au solution and the concentration that contains the variable concentrations sulphion be 0.01 mol/L, contain the thiocarbamide of 5 μ mol/L, the phosphate buffered solution of pH=9.0 is mixed, 30 ℃ were reacted 3 minutes, visualization color characteristic or mensuration absorbance ratio A
680/ A
520To judge the concentration of sulphion; Nano-Au solution is respectively 0.2 milliliter and 0.2 milliliter with the phosphate buffered solution volume that contains the variable concentrations sulphion.
7. one kind
The rapid assay methods of sulphion in colour developing probe assay mineral water or tap water sample or the river sample take nm of gold as logic gate, comprise the steps: to get mineral water sample or tap water sample or river sample, at first use 0.22 micron membrane filtration, then in filtrate, add EDTA to final concentration be 0.1 mmol/L; Filtrate is regulated its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and make sample solution; 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
8. one kind
The rapid assay methods of sulphion in the colour developing probe assay white wine take nm of gold as logic gate, comprise the steps: the white wine sample add EDTA to final concentration be 0.1 mmol/L, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and form sample solution; 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
9. one kind
The rapid assay methods of sulphion in the colour developing probe assay human urine take nm of gold as logic gateComprise the steps: the human urine sample add EDTA to final concentration be 0.1 mmol/L, regulate its pH value to 9.0 with 2 mol/L NaOH solution, and add the thiocarbamide of 5 μ mol/L and form sample solution, 0.2 add 0.2 milliliter of above-mentioned sample solution in the milliliter nm of gold, 30 ℃ were reacted 3 minutes, then visualization change color or mensuration absorbance ratio A
680/ A
520, carry out quantitatively according to solution colour and color standards series of comparisons or by the absorbance ratio typical curve, judge
The content of the sulphion in the sample
10. according to claim 7 or 8 or 9 described
The rapid assay methods of the sulphion of colour developing probe take nm of gold as logic gateIt is characterized in that employed nm of gold adopts the method preparation of sodium citrate reduction gold chloride, the chlorauric acid solution of 1 milliliter of 0.1 g/L is dissolved in 100 ml waters, reflux heating boils the citric acid three sodium solution of 3 milliliter of 0.1 g/L of rear rapid adding, reaction solution is from the light yellow claret that becomes, after continuing to reflux 15 minutes, reaction solution slowly is cooled to room temperature and forms nm of gold.
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| CN104215594A (en) * | 2014-09-15 | 2014-12-17 | 天津师范大学 | Method for detecting hazardous substance-thiourea by utilizing gold nanoparticles |
| CN105785688A (en) * | 2016-05-02 | 2016-07-20 | 福建医科大学 | NOR solid-phase logic gate based on gold nanocluster-polyelectrolyte compound |
| CN105842215A (en) * | 2016-05-02 | 2016-08-10 | 福建医科大学 | NAND logic gate based on BSA/3-MPA-Au nanoclusters and establishment method of NAND logic gate |
| CN105954244A (en) * | 2016-05-02 | 2016-09-21 | 福建医科大学 | BSA/3-MPA-gold nanocluster-based NAND+NOR composite logic gate and construction method thereof |
| CN105954245A (en) * | 2016-05-02 | 2016-09-21 | 福建医科大学 | BSA/3-MPA-gold nanocluster-based NOR logic gate and construction method thereof |
| CN108508002A (en) * | 2018-02-08 | 2018-09-07 | 暨南大学 | A kind of NIMPLY+OR logic gates based on nanogold and the Visual retrieval applied to DNA |
| CN108732119A (en) * | 2018-02-08 | 2018-11-02 | 暨南大学 | A kind of NIMPLY logic gates based on nanogold and the Visual retrieval applied to DNA |
| CN111157521A (en) * | 2020-02-15 | 2020-05-15 | 信阳学院 | Nano-gold colorimetric method based on agglomeration resistance and determination of silver ions |
| WO2024065049A1 (en) * | 2022-09-28 | 2024-04-04 | Hanna Gerald | Method and system for detecting cationic and anionic polymers in aqueous solutions |
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