CN110261604A - The preparation method and application of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone - Google Patents
The preparation method and application of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone Download PDFInfo
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- CN110261604A CN110261604A CN201910659840.1A CN201910659840A CN110261604A CN 110261604 A CN110261604 A CN 110261604A CN 201910659840 A CN201910659840 A CN 201910659840A CN 110261604 A CN110261604 A CN 110261604A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54346—Nanoparticles
Abstract
The preparation method of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone, the preparation method is that: the mesoporous silica nano particle that polyethyleneimine wraps up is added to the phosphate buffer solution containing pyrroloquinoline quinone, is shaken 12 hours or so；Then the phosphate buffer solution containing detection antibody is added, continues concussion reaction 1 hour or so, centrifuge washing, lower sediment is scattered in phosphate buffer solution, saves backup at 4 DEG C；Before use, being diluted with the phosphate buffer solution containing bovine serum albumin(BSA).The mesoporous silica nano particle of the application of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone, the antibody functionalized load pyrroloquinoline quinone is detected for antigen.Simple and sensitive of the present invention, it is intuitive, high-throughput, do not need the advantages that specific apparatus.
Description
Technical field
The present invention relates to immunoassay detection, in particular to a kind of antibody functionalized load for immunoassay detection
The mesoporous silica nano particle and detection method of pyrroloquinoline quinone, belong to chemical field.
Background technique
Visualization immunoassay have the characteristics that it is easy to operate, do not need specific apparatus, various diseases prevention, examine
It is had received widespread attention in disconnected and treatment.Currently, the antibody that standardized visualization immunoassay is marked usually using enzyme
Carry out Catalytic color reaction, and then achievees the purpose that quantitative analysis.However, in practical applications, native enzyme there are problems, than
Such as thermal stability is poor, easily affected by environment, preparation and purification are costly, sensitivity is low.
With the development of nanosecond science and technology and nano material, based on nano material visualization immunoassay sensitivity and
Stability is obviously improved.In general, effect of the nano material in visualization immunoassay experiment can be divided into four
Seed type: as nano enzyme Catalytic color reaction；As chromogenic substrate, nano material aggregation or pattern are caused by target molecule
The variation of size；As carrier, a large amount of native enzyme or color developing agent are loaded；As presoma, releasing being capable of catalyzed coloration
The metal ion of reaction.Wherein, nano enzyme has many advantages, such as that at low cost, catalytic activity is controllable, stability is high.Nano enzyme mainly wraps
Include the nano materials such as carbon, metal and metal oxide.In the catalysis reaction of glucose and hydrogen peroxide, they usually play class
It is similar to the function of oxidizing ferment, catalase or mutase.Immunoassay method based on nano enzyme is simple and effective, still, receives
There are still some problems in terms of the practical application of visualization immunoassay for rice enzyme.For example, largely being received compared to native enzyme
Rice enzyme has lower catalytic activity, weak binding force and poor substrate specificity.During the experiment, it needs to nanometer material
The surface of material is modified, to carry out molecular recognition and reduce non-specific adsorption.However, these ornamental equivalents would generally drop
The catalytic activity of low nano enzyme (especially metal and metal oxide-type nano material).Therefore, it constructs immune for visualizing
The novel nano enzyme or nano catalystic system of analysis are of great significance.
Summary of the invention
It is an object of the invention to overcome drawbacks described above present in current antigen detection method, a kind of antibody function is provided
The preparation method and application of the mesoporous silica nano particle of the load pyrroloquinoline quinone of energyization.
To achieve the purpose of the present invention, using following technical solutions: antibody functionalized load pyrroloquinoline quinone
The preparation method of mesoporous silica nano particle, the preparation method is that: the mesoporous silica that polyethyleneimine is wrapped up
Nano particle is added to the phosphate buffer solution containing pyrroloquinoline quinone, is shaken 12 hours or so；Then it is added containing detection
The phosphate buffer solution of antibody continues concussion reaction 1 hour or so, and centrifuge washing, lower sediment is scattered in phosphate buffer solution,
It is saved backup at 4 DEG C；Before use, being diluted with the phosphate buffer solution containing bovine serum albumin(BSA).
Further；The mesoporous silica nano particle of the polyethyleneimine package is prepared using following methods:
A: the preparation of mesoporous silica nano particle: cetyl trimethylammonium bromide is dissolved in ultrapure water, then plus
Enter sodium hydroxide solution, 15 min are stirred to react at 80 DEG C, is slow added into tetraethoxysilane solution, is vigorously stirred 20
Obtained white precipitate is filtered, is successively washed with water and methanol by min, dry at 80 DEG C；Solid dispersion after drying
In the mixed solution of hydrochloric acid and methanol, back flow reaction 12 hours or so, is washed with water and methanol, carried out at 60 DEG C again
It is dried in vacuo to get the nano SiO 2 particle of central hole structure is arrived；
B: the preparation of the mesoporous silica nano particle of polyethyleneimine package: the mesoporous being prepared in step (1)
Nano SiO 2 particle is added in aq. polyethyleneimine, is stirred to react 12 hours or so, centrifuge washing, at 60 DEG C
Under be dried in vacuo.
The application of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone, the antibody function
The mesoporous silica nano particle of the load pyrroloquinoline quinone of change is detected for antigen.
Further；Specific method step for antigen detection are as follows: be added into the magnetic ball of Streptavidin functionalization
Phosphate buffer solution containing biotin functionalized capture antibody, is reacted 30 ~ 60 minutes, is separated using magnet, use phosphoric acid
Buffer solution washing, discards supernatant liquor, lower sediment is scattered in phosphate buffer solution；Then the phosphoric acid buffer containing antigen is added
Solution, mixing concussion, is incubated for；Magnetic separation and washing, lower sediment are scattered in phosphate buffer solution, add detection antibody function
The mesoporous silica nano particle and bovine serum albumin(BSA) mixed solution of the load pyrroloquinoline quinone of energyization, concussion reaction；Magnetic
Power separation and washing, lower sediment are scattered in containing three (2- carboxyethyl) phosphines and ferric ion-phenanthrene piperazine complex compound phosphoric acid buffer
Solution, the solution colour that detects by an unaided eye variation or the absorption with ultraviolet-uisible spectrophotometer measurement solution at 562 nm
Value.
Further；Before the mesoporous silica nano particle of the antibody functionalized load pyrroloquinoline quinone is used for
The detection of column gland specific antigen, specific detection method are as follows: function containing biotin is added into the magnetic ball of Streptavidin functionalization
The phosphate buffer solution of the capture antibody of the prostate-specific antigen of change, is reacted 30 ~ 60 minutes, is separated using magnet,
It is washed with phosphate buffer solution, discards supernatant liquor, lower sediment is scattered in phosphate buffer solution；The capture antibody of preparation is taken to repair
The magnetic ball of decorations, is added the phosphate buffer solution containing prostate-specific antigen, and mixing concussion is incubated for 30 minutes or so；Magnetic force point
From and washing, the mesoporous silica nano particle and bovine serum albumin for detecting antibody functionalized load pyrroloquinoline quinone is added
White mixed solution, the antibody in the mesoporous silica nano particle of the antibody functionalized load pyrroloquinoline quinone of the described detection
For the antibody of prostate-specific antigen, concussion reaction 30 minutes or so；It magnetic separation and washes twice, lower sediment is scattered in
Containing three (2- carboxyethyl) phosphines and ferric ion-phenanthrene piperazine complex compound phosphate buffer solution, the solution colour that detects by an unaided eye variation,
Or the absorption value with spectrophotometric determination solution at 562 nm.
This technology invents positive advantageous effects are as follows: (1) nano SiO 2 particle of mesoporous can load largely
Load pyrroloquinoline quinone；(2) it is catalyzed between three (2- carboxyethyl) phosphines and ferric ion-phenanthrene piperazine complex compound by pyrroloquinoline quinone
Redox cycle reaction, establishes the colorimetric methods for antigen detection, have simple and sensitive, it is intuitive, high-throughput, be not required to
The advantages that wanting specific apparatus, succeeding in developing for the technology will realize the detection of different types of antigens.
Detailed description of the invention
Fig. 1 is the transmission electron microscope picture of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone.
Fig. 2 be the biotin functionalized capture antibody of the magnetic ball warp of Streptavidin functionalization, prostate-specific antigen,
Transmission electron microscope picture after the mesoporous silica nano particle processing step of antibody functionalized load pyrroloquinoline quinone.
Fig. 3 is the ultraviolet-visible absorption spectroscopy curve of solution in the presence of the prostate-specific antigen of various concentration.
Fig. 4 is the relation curve of absorption value Yu prostate-specific antigen concentration.
Fig. 5 is the selectivity diagram of the analysis method.
Specific embodiment
In order to more fully explain implementation of the invention, embodiment of the invention is provided.These embodiments are only
Elaboration to the technique, does not limit the scope of the invention, and is illustrated in the present invention with following embodiment, but be not limited to following implementations
Example, any change are included in technical scope of the invention.
Antibody is the antibody of prostate-specific antigen in the present invention.Following embodiment is by taking prostate-specific antigen as an example
It is specifically addressed.To attached drawing further explanation, Fig. 1 is the mesoporous silica of antibody functionalized load pyrroloquinoline quinone
The transmission electron microscope picture of nano particle.From figure 1 it appears that synthesized nano particle dispersibility is preferably, having a size of the left side 80 nm
It is right.
Fig. 2 be the biotin functionalized capture antibody of the magnetic ball warp of Streptavidin functionalization, prostate-specific antigen,
Projection electron microscope after the mesoporous silica nano particle processing step of antibody functionalized load pyrroloquinoline quinone.From figure
As can be seen that the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone is by magnetic capture.
Fig. 3 is the ultraviolet-visible absorption spectroscopy curve of solution in the presence of the prostate-specific antigen of various concentration, preceding
The concentration of column gland specific antigen is successively 0,0.005,0.05,0.125,0.25,0.5,1,2 ng/mL.Fig. 4 is
The relation curve of absorption value and prostate-specific antigen concentration, the concentration of prostate-specific antigen are successively 0.005,
0.05,0.125,0.25,0.5,1,2 ng/mL, the range of linearity are that 0.005 ~ 0.5 ng/mL(is shown in inserting in Fig. 4
Figure).Fig. 5 is influence of the different proteins to ultraviolet absorption value, is corresponding in turn to from 1 to 6: the immunoglobulin of 50 ng/mL
G, the alpha-fetoprotein of 50 ng/mL, the carcinomebryonic antigen of 50 ng/mL, the fibrin ferment of 50 ng/mL, blank sample, 2.5 ng/mL's
Prostate-specific antigen.The mesoporous silica nano particle that polyethyleneimine wraps up in the present invention is in current technical literature
In there is mature technical solution.
One, the preparation of the magnetic ball of polypeptide functionalization:
A: it is ultrapure that 50 mg cetyl trimethylammonium bromides the preparation of mesoporous silica nano particle: are dissolved in 200 mL
In water, the 2 M sodium hydroxide solutions of 1.75 mL are then added, 15 min are stirred to react at 80 DEG C, are slow added into 2.5
ML tetraethoxysilane solution, is vigorously stirred 20 min, and obtained white precipitate is filtered, is successively washed with water and methanol, then
It is dry at 80 DEG C；Solid after drying is scattered in the mixed solution of hydrochloric acid (0.5 mL) and methanol (50 mL), and reflux is anti-
It answers 12 hours or so, is washed again with water and methanol, is dried in vacuo at 60 DEG C to get the titanium dioxide of central hole structure is arrived
Nano silicon particles；
B: it the preparation of the mesoporous silica nano particle of polyethyleneimine package: will be prepared in 500 mg steps (1)
Mesoporous silica nano particle be added in 300 mg aq. polyethyleneimines, be stirred to react 12 hours or so, be centrifuged
Washing, is dried in vacuo at 60 DEG C.
Two, the preparation of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone:
The mesoporous silica nano particle that 1 mg polyethyleneimine wraps up is added to, 1 mL is contained into pyrroloquinoline quinone (12 μ
M phosphate buffer solution) shakes 12 hours or so；Then the detection antibody (50 that 1 mL contains prostate-specific antigen is added
μ g) phosphate buffer solution, continue concussion reaction 1 hour or so, 15 min, ultrapure washing are centrifuged under the conditions of 5000 rpm
It washs, lower sediment is scattered in 1 mL phosphate buffer solution (50 mM, pH 7.4), saves backup at 4 DEG C；Before use, with containing
There is the phosphate buffer solution of bovine serum albumin(BSA) (0.1% w/v) to dilute.
Three, the detection of prostate-specific antigen
The 20 μ g/mL that the mL containing l is added into the magnetic ball of the 200 μ g/mL Streptavidin functionalization of l mL are biotin functionalized
Prostate-specific antigen capture antibody phosphate buffer solution, react 30 ~ 60 minutes or so, divided using magnet
From being washed with phosphate buffer solution, discard supernatant liquor, lower sediment is scattered in 4 mL phosphate buffer solutions；90 μ L are taken to prepare
Capture antibody modification magnetic ball, be added 10 phosphate buffer solutions of the μ L containing prostate-specific antigen, mixing concussion, be incubated for
30 minutes or so；The mesoporous titanium dioxide that 50 μ L detect antibody functionalized load pyrroloquinoline quinone is added in magnetic separation and washing
Nano silicon particles and bovine serum albumin(BSA) mixed solution, concussion reaction 30 minutes or so；It magnetic separation and washes twice, lower layer is heavy
It is slow containing three (2- carboxyethyl) phosphines (0.5 mM) and ferric ion-phenanthrene piperazine complex compound (0.2 mM) phosphoric acid that shallow lake is scattered in 100 μ L
Solution (pH 8.6) is rushed, the solution colour that finally detects by an unaided eye variation, or with spectrophotometric determination solution at 562 nm
Absorption value.Fig. 3 is the ultraviolet-visible absorption spectroscopy of solution in the presence of the prostate-specific antigen of various concentration, as a result
Show that the concentration of prostate-specific antigen is bigger, solution colour is more and more redder, and the absorption spectrum intensity of solution is stronger, the suction
Receipts are generated by the ferrous ion-phenanthrene piperazine complex compound generated in solution, when the concentration of prostate-specific antigen is higher than
When 0.05 ng/mL, the variation of solution colour with the naked eye can be observed；Fig. 4 be absorption value of the solution at 562 nm from it is different
The relational graph of concentration prostate-specific antigen detection, it can be seen that with the increase of concentration, the absorption value of solution is bigger, table
The variation of the bright ferrous ion according to generation-phenanthrene piperazine complex compound absorption intensity, can measure the dense of prostate-specific antigen
Degree, can detecte to 0.005 ng/mL prostate-specific antigen, before showing that this method can be used for using spectrophotometer
The colorimetric analysis of column gland specific antigen detects, and the range of linearity is 0.005 ~ 0.5 ng/mL.
Embodiment 3: selectivity
Prostate-specific antigen is changed into substance to be tested, other step condition does not change referring to 2 step of embodiment by step
Become, experimental result such as Fig. 5.The result shows that only prostate-specific antigen can generate stronger absorption value, solution is caused to become
For aubergine, other materials do not cause solution colour to change, and the variation of ultraviolet absorption value caused by these substances can be ignored not
Meter shows that this method has preferable selectivity to the detection of prostate-specific antigen.
Exploration of Mechanism and analysis of the invention: pyrroloquinoline quinone can be restored by three (2- carboxyethyl) phosphines, reduzate into
And colourless ferric ion-phenanthrene piperazine complex compound is reduced to ferrous ion-phenanthrene piperazine complex compound of aubergine；And the pyrrole generated
It coughs up quinoline quinone to be restored by three (2- carboxyethyl) phosphines again, starts again at and reacted with ferric ion-phenanthrene piperazine complex compound, repeatedly followed
After ring, a large amount of ferrous ion-phenanthrene piperazine complex compound will be generated, to form purplish red solution.Based on this principle, work as sample
In there are when prostate-specific antigen, load pyrroloquinoline quinone mesoporous silica nano particle will by magnetic capture, bear
The pyrroloquinoline quinone of load can restore colourless ferric ion-phenanthrene piperazine complex compound, the empurpled ferrous ion of shape-phenanthrene piperazine
Complex compound.This change procedure can be monitored using naked eyes or spectrophotometer.The specific technical proposal is: to Streptavidin
The phosphate buffer solution containing biotin functionalized capture antibody is added in the magnetic ball of functionalization, reacts 30 ~ 60 minutes, uses
Magnet is separated, and is washed with phosphate buffer solution, and supernatant liquor is discarded, and lower sediment is scattered in phosphate buffer solution；Then
The phosphate buffer solution containing antigen is added, mixing concussion is incubated for；Magnetic separation and washing, lower sediment are scattered in phosphoric acid buffer
Solution adds the mesoporous silica nano particle and bovine serum albumin(BSA) for detecting antibody functionalized load pyrroloquinoline quinone
Mixed solution, concussion reaction；Magnetic separation and washing, lower sediment are scattered in containing three (2- carboxyethyl) phosphines and ferric ion-
The phosphate buffer solution of luxuriant and rich with fragrance piperazine complex compound, the solution colour that detects by an unaided eye variation or molten with ultraviolet-uisible spectrophotometer measurement
Absorption value of the liquid at 562 nm.
After the embodiment that the present invention will be described in detail, one of ordinary skilled in the art is clearly understood that, is not being taken off
It is lower from above-mentioned claim and spirit to carry out various change and modify, it is all according to the technical essence of the invention to the above reality
Any simple modification, equivalent change and modification made by example are applied, belong to the range of technical solution of the present invention, and the present invention is also not
It is limited to the embodiment of example in specification.
Claims (5)
1. the preparation method of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone, it is characterised in that institute
It is stating the preparation method comprises the following steps: the mesoporous silica nano particle that polyethyleneimine is wrapped up be added to will be containing pyrroloquinoline quinone
Phosphate buffer solution shakes 12 hours or so；Then the phosphate buffer solution containing detection antibody is added, continues concussion reaction 1
Hour or so, centrifuge washing, lower sediment is scattered in phosphate buffer solution, saves backup at 4 DEG C；Before use, with ox is contained
Sero-abluminous phosphate buffer solution dilution.
2. the system of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone according to claim 1
Preparation Method, it is characterised in that: the mesoporous silica nano particle of the polyethyleneimine package is prepared using following methods:
A: the preparation of mesoporous silica nano particle: cetyl trimethylammonium bromide is dissolved in ultrapure water, then plus
Enter sodium hydroxide solution, 15 min are stirred to react at 80 DEG C, is slow added into tetraethoxysilane solution, is vigorously stirred 20
Obtained white precipitate is filtered, is successively washed with water and methanol by min, dry at 80 DEG C；Solid dispersion after drying
In the mixed solution of hydrochloric acid and methanol, back flow reaction 12 hours or so, washed again with water and methanol, 60 under vacuum condition
It is DEG C dry to get the nano SiO 2 particle for arriving central hole structure；
B: the preparation of the mesoporous silica nano particle of polyethyleneimine package: the mesoporous being prepared in step (1)
Nano SiO 2 particle is added in aq. polyethyleneimine, is stirred to react 12 hours or so, centrifuge washing, vacuum condition
Lower 60 DEG C of dryings.
3. the application of the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone, it is characterised in that: described
Antibody functionalized load pyrroloquinoline quinone mesoporous silica nano particle for antigen detect.
4. the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone according to claim 3 is answered
With, it is characterised in that: the specific method step for antigen detection are as follows: be added into the magnetic ball of Streptavidin functionalization containing life
The phosphate buffer solution of the capture antibody of the antigen of object element functionalization, is reacted 30 ~ 60 minutes, is separated using magnet, use phosphorus
The washing of acid buffering solution, discards supernatant liquor, lower sediment is scattered in phosphate buffer solution；Then it is slow that the phosphoric acid containing antigen is added
Solution is rushed, mixing concussion is incubated for；Magnetic separation and washing, lower sediment are scattered in phosphate buffer solution, add detection antibody
The mesoporous silica nano particle and bovine serum albumin(BSA) mixed solution of the load pyrroloquinoline quinone of functionalization, concussion reaction；
Magnetic separation and washing, lower sediment are scattered in slow containing three (2- carboxyethyl) phosphines and ferric ion-phenanthrene piperazine complex compound phosphoric acid
Solution is rushed, the solution colour that detects by an unaided eye variation or the absorption with ultraviolet-uisible spectrophotometer measurement solution at 562 nm
Value.
5. the mesoporous silica nano particle of antibody functionalized load pyrroloquinoline quinone according to claim 3 is answered
With, it is characterised in that: the mesoporous silica nano particle of the antibody functionalized load pyrroloquinoline quinone is used for forefront
The detection of gland specific antigen, specific detection method are as follows: be added into the magnetic ball of Streptavidin functionalization containing biotin functionalized
Prostate-specific antigen capture antibody phosphate buffer solution, react 30 ~ 60 minutes, separated using magnet, use
Phosphate buffer solution washing, discards supernatant liquor, lower sediment is scattered in phosphate buffer solution；Take the capture antibody modification of preparation
Magnetic ball, be added the phosphate buffer solution containing prostate-specific antigen, mixing concussion, be incubated for 30 minutes；It magnetic separation and washes
It washs, the mesoporous silica nano particle for detecting antibody functionalized load pyrroloquinoline quinone and bovine serum albumin(BSA) mixing is added
Solution, the antibody in the mesoporous silica nano particle of the antibody functionalized load pyrroloquinoline quinone of the described detection is forefront
The antibody of gland specific antigen, concussion reaction 30 minutes；It magnetic separation and washes twice, lower sediment is scattered in containing three (2- carboxylics
Ethyl) phosphine and ferric ion-phenanthrene piperazine complex compound phosphate buffer solution, the solution colour that detects by an unaided eye variation, or with dividing
Absorption value of the light photometric determination solution at 562 nm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111097045A (en) * | 2020-01-15 | 2020-05-05 | 华南理工大学 | Bifunctional nanoparticle and preparation method and application thereof |
| CN115317515A (en) * | 2022-07-19 | 2022-11-11 | 沈阳药科大学 | Multi-effect synergetic biomimetic mineralized nano preparation of lonidamine/bovine serum albumin/iron phosphate and preparation method and application thereof |
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| CN106474493A (en) * | 2016-09-22 | 2017-03-08 | 苏州大学 | Diagnosis and treatment integration nano-probe for bacterial infections in vivo and preparation method thereof |
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| CN106474493A (en) * | 2016-09-22 | 2017-03-08 | 苏州大学 | Diagnosis and treatment integration nano-probe for bacterial infections in vivo and preparation method thereof |
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| EUGENII KATZ,ET AL.: "Amperometric amplification of antigen-antibody association at monolayer interfaces: design of immunosensor electrodes", 《JOURNAL OF ELECTROANALYTICAL CHEMISTRY》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111097045A (en) * | 2020-01-15 | 2020-05-05 | 华南理工大学 | Bifunctional nanoparticle and preparation method and application thereof |
| CN111097045B (en) * | 2020-01-15 | 2022-11-18 | 华南理工大学 | Bifunctional nanoparticle and preparation method and application thereof |
| CN115317515A (en) * | 2022-07-19 | 2022-11-11 | 沈阳药科大学 | Multi-effect synergetic biomimetic mineralized nano preparation of lonidamine/bovine serum albumin/iron phosphate and preparation method and application thereof |
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