CN105832770A - Method for producing calculus bovis by condensation method - Google Patents
Method for producing calculus bovis by condensation method Download PDFInfo
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- CN105832770A CN105832770A CN201610251744.XA CN201610251744A CN105832770A CN 105832770 A CN105832770 A CN 105832770A CN 201610251744 A CN201610251744 A CN 201610251744A CN 105832770 A CN105832770 A CN 105832770A
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- calculus bovis
- bovis
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- calculus
- seu bubali
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/413—Gall bladder; Bile
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Abstract
The invention discloses a method for producing calculus bovis by a condensation method. The method comprises the following steps of: adding 0.05g of superfine granular calculus bovis into every 1000ml of fresh oxgall to serve as a condensation kernel; heating the fresh oxgall and superfine granular calculus bovis to 39 DEG C, and regulating the pH value to be between 4.5 and 5.5; adding 0.05g of calcium hydrophosphate and 3g of cholesterol into every 1000ml of fresh calculus bovis, performing ultrasonic vibration for 8 minutes; cooling to 35 DEG C, stirring for 20 minutes, heating to 50 DEG C, and cooling to normal temperature; and precipitating for 8 hours to obtain particles which are the prepared calculus bovis. According to the method, the calculus bovis is prepared by simulating the physiological environment in the ox-gall based on a bionic principle serving as a basic thought, has the same ingredient with that of natural calculus bovis. The method for producing calculus bovis has low cost and simple equipment; the product can be easily transferred into productive force, can access to market with high speed and high benefit, and can be used by broad doctors and patients in time.
Description
Technical field
A kind of method that the present invention relates to In vitro cultured Calculus Bovis, particularly relates to a kind of coagulation and produces the side of Calculus Bovis
Method.
Background technology
Calculus Bovis is the rare Chinese medicine that a kind of bitterness is cold and cool, has heat clearing away, removing toxic substances, function of gallbladder promoting, the effect such as relieving convulsion.
It is applicable to high heat, tetany spasm, laryngopharynx swelling and pain, the aphtha of the mouth and tongue etc. of acute calenture.Natural Calculus Bovis
Natural production rate is extremely low.Universal along with mechanization of agriculture after twentieth century, cattle does not recultivates, cowboying is with food
Meat is main, and the old cattle of more than 5 years is little, and natural Calculus Bovis generates the most very little, and medicine source scarcity is totally.
So far national drug superintendent office have approved natural Calculus Bovis succedaneum successively, i.e. artificial Calculus Bovis, Calculus Bovis cultivating with
And In vitro cultured Calculus Bovis.More existing In vitro cultured Calculus Bovis methods, complex process, production cost are high, product
Measure and low be unfavorable for large batch of production.
Summary of the invention
The purpose of the present invention is that the side providing a kind of coagulation to produce Calculus Bovis to solve the problems referred to above
Method.
The present invention is achieved through the following technical solutions above-mentioned purpose:
A kind of coagulation produces the method for Calculus Bovis, it is characterised in that comprise the following steps:
One, take fresh Fel Bovis seu Bubali, fresh Fel Bovis seu Bubali adds ultra-fine grain Calculus Bovis, the fresh cattle of every 1000ml
Bile adds ultra-fine grain Calculus Bovis 0.05g, as the nuclei of condensation, enters next step；
Two, fresh Fel Bovis seu Bubali and ultra-fine grain Calculus Bovis are heated to 39 DEG C, and regulate pH value 4.5-5.5 it
Between, enter next step；
Three, the fresh Fel Bovis seu Bubali of every 1000ml adds calcium hydrogen phosphate 0.05g and cholesterol 3g, ultrasonic wave concussion 8
Minute, enter next step；
Four, it is cooled to 35 DEG C, stirs 20 minutes, be heated to 50 DEG C, then treat that it is down to room temperature, under entrance
One step；
Five, precipitation 8 hours, the granule that precipitation obtains is the Calculus Bovis produced.
2, the method that a kind of coagulation according to claim 1 produces Calculus Bovis, it is characterised in that: in power
Profit requires to regulate pH value with hydrochloric acid in the step 2 of 1.
The beneficial effects of the present invention is:
The invention provides a kind of method that coagulation produces Calculus Bovis, with bionics principle as basic ideas, mould
Physiological environment in imitative Fel Bovis seu Bubali and output, composition is identical with natural Calculus Bovis, with the method production Calculus Bovis cost
Low, equipment is simple, its achievement is readily converted to the productivity, and product comes into the market, and speed is fast, high efficiency, energy
Used by numerous doctors patient in time.
Detailed description of the invention
A kind of coagulation produces the method for Calculus Bovis, it is characterised in that comprise the following steps:
One, take fresh Fel Bovis seu Bubali, fresh Fel Bovis seu Bubali adds ultra-fine grain Calculus Bovis, the fresh cattle of every 1000ml
Bile adds ultra-fine grain Calculus Bovis 0.05g, as the nuclei of condensation, enters next step；
Two, fresh Fel Bovis seu Bubali and ultra-fine grain Calculus Bovis are heated to 39 DEG C, and regulate pH value 4.5-5.5 it
Between (regulation pH value hydrochloric acid), enter next step；
Three, the fresh Fel Bovis seu Bubali of every 1000ml adds calcium hydrogen phosphate 0.05g and cholesterol 3g, ultrasonic wave concussion 8
Minute, enter next step；
Four, it is cooled to 35 DEG C, stirs 20 minutes, be heated to 50 DEG C, then treat that it is down to room temperature, under entrance
One step；
Five, precipitation 8 hours, the granule that precipitation obtains is the Calculus Bovis produced.
Step 2 regulates pH value with hydrochloric acid.
It is brown color loose particles shape with the Calculus Bovis of this production technology output, bitter in the mouth.By Chinese Pharmacopoeia (1985
Year version) compare with natural Calculus Bovis: equal at extension first experiment, furfural sulphuric acid, chloroform sulphuric acid, hydroperoxidation
Consistent with natural Calculus Bovis.Through Beijing Toilet Clinical Medicine Inst. check, with this law produce Calculus Bovis with
Chinese Pharmacopoeia one contrast of version in 2010: cholic acid (dry product), bilirubin (dry product) etc. all meet regulation.
Technical scheme is not limited to the restriction of above-mentioned specific embodiment, every technology according to the present invention
The technology deformation that scheme is made, within each falling within protection scope of the present invention.
Claims (2)
1. the method that a coagulation produces Calculus Bovis, it is characterised in that comprise the following steps:
One, take fresh Fel Bovis seu Bubali, fresh Fel Bovis seu Bubali adds ultra-fine grain Calculus Bovis, the fresh cattle of every 1000ml
Bile adds ultra-fine grain Calculus Bovis 0.05g, as the nuclei of condensation, enters next step；
Two, fresh Fel Bovis seu Bubali and ultra-fine grain Calculus Bovis are heated to 39 DEG C, and regulate pH value 4.5-5.5 it
Between, enter next step；
Three, the fresh Fel Bovis seu Bubali of every 1000ml adds calcium hydrogen phosphate 0.05g and cholesterol 3g, ultrasonic wave concussion 8
Minute, enter next step；
Four, it is cooled to 35 DEG C, stirs 20 minutes, be heated to 50 DEG C, then treat that it is down to room temperature, under entrance
One step；
Five, precipitation 8 hours, the granule that precipitation obtains is the Calculus Bovis produced.
A kind of coagulation the most according to claim 1 produces the method for Calculus Bovis, it is characterised in that: in power
Profit requires to regulate pH value with hydrochloric acid in the step 2 of 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610251744.XA CN105832770A (en) | 2016-04-22 | 2016-04-22 | Method for producing calculus bovis by condensation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610251744.XA CN105832770A (en) | 2016-04-22 | 2016-04-22 | Method for producing calculus bovis by condensation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105832770A true CN105832770A (en) | 2016-08-10 |
Family
ID=56588988
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610251744.XA Pending CN105832770A (en) | 2016-04-22 | 2016-04-22 | Method for producing calculus bovis by condensation method |
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| CN (1) | CN105832770A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1626729A2 (en) * | 2003-05-29 | 2006-02-22 | Bio-Defense Nutritionals, Inc. | Compositions and methods for symptomatic relief |
| CN1813800A (en) * | 2005-02-01 | 2006-08-09 | 徐华民 | Process for preparing cattle choleithiasia by external ultrasonic directional condensation |
| CN103316046A (en) * | 2013-06-09 | 2013-09-25 | 宁波优美肽生物科技有限公司 | Enzymatic production process for culturing calculus bovis in vitro |
| EP3072517A4 (en) * | 2013-11-22 | 2017-05-17 | Fu, Li | Use of ginsenoside-rg3 in preparing medicine for preventing or/and treating dementia and medicine |
| CN111388132A (en) * | 2020-04-11 | 2020-07-10 | 西安国联质量检测技术股份有限公司 | Bezoar generator for external gallbladder of cow and method for preparing bezoar by using bezoar generator |
-
2016
- 2016-04-22 CN CN201610251744.XA patent/CN105832770A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1626729A2 (en) * | 2003-05-29 | 2006-02-22 | Bio-Defense Nutritionals, Inc. | Compositions and methods for symptomatic relief |
| CN1813800A (en) * | 2005-02-01 | 2006-08-09 | 徐华民 | Process for preparing cattle choleithiasia by external ultrasonic directional condensation |
| CN103316046A (en) * | 2013-06-09 | 2013-09-25 | 宁波优美肽生物科技有限公司 | Enzymatic production process for culturing calculus bovis in vitro |
| EP3072517A4 (en) * | 2013-11-22 | 2017-05-17 | Fu, Li | Use of ginsenoside-rg3 in preparing medicine for preventing or/and treating dementia and medicine |
| CN111388132A (en) * | 2020-04-11 | 2020-07-10 | 西安国联质量检测技术股份有限公司 | Bezoar generator for external gallbladder of cow and method for preparing bezoar by using bezoar generator |
Non-Patent Citations (5)
| Title |
|---|
| O G NIEBEN等: ""Intragastric ballon as an artificial bezoar for treatment of obesity"", 《LANCET》 * |
| 俞长芳编: "《胆汁、牛黄与动物结石》", 28 February 1991 * |
| 张永海: "ConABP对牛黄成核与核生长的影响"", 《中国优秀硕士学位论文全文数据库（电子期刊）》 * |
| 易美华编: "《生物资源开发利用》", 30 June 2003 * |
| 袁生馨等: ""人工培植牛黄简介"", 《青海畜牧兽医杂志》 * |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160810 |