CN111388132A - Bezoar generator for external gallbladder of cow and method for preparing bezoar by using bezoar generator - Google Patents

Abstract

The invention provides a cow external gallbladder bezoar generator; the method comprises the following steps: the device comprises a generation device, a driving device and a recovery device, wherein the generation device comprises a shell, an inner container which is arranged at the bottom of the shell and is provided with an accommodating space, and a yellow bed which is accommodated in the accommodating space, the yellow bed is of an inverted umbrella-shaped structure, the tip end of the yellow bed is arranged on one side of the inner container, the driving device comprises a transmission shaft, and the transmission shaft penetrates through the inner container, the end part of the yellow bed and the inner container to be in driving connection. The invention also provides a method for preparing bezoar by the bezoar vitro gallbladder bezoar generator. The bezoar generating device for the external gallbladder of the cattle provided by the invention processes the bezoar generating device for the gallbladder by utilizing the principle of gallbladder hydrodynamics when natural bezoar is generated, so that bile does centripetal rocking motion in the gallbladder, the aggregation of tiny bezoar particles is accelerated, and the bezoar yield is increased.

Description

Bezoar generator for external gallbladder of cow and method for preparing bezoar by using bezoar generator

Technical Field

The invention relates to the field of cultivated calculus bovis, in particular to an external-gallbladder calculus bovis generator for cattle and a method for preparing calculus bovis by using the same.

Background

Bezoar is calculus in gall bladder, bile duct and hepatic duct of animals of the family bovidae, is a rare traditional Chinese medicine, has a rare yield and a high price (higher than gold), and has been used for treating diseases for two thousand years in China. The main effects of bezoar are clearing away heat and toxic material, tranquilizing the mind, inducing resuscitation and resisting convulsion; has unique curative effect on hyperpyrexia, convulsion, coma and swollen sore throat. It is the main medicine of Chinese patent medicines such as Angongniuhuang pills, Liushen pills, and Niuhuang Jiedu pills. According to statistics, more than 150 rare Chinese patent medicines in China have bezoar in 270 prescriptions. However, the natural bezoar is still slight, and only 129 cattle have been found to have natural bezoar by investigation of 61428 cattle, and the yellowing rate is 0.21%. Obviously, the requirement of medicine is far from being met by obtaining calculus bovis only depending on the slaughter rate.

In the early 70 s, Chinese medical workers developed artificial bezoar by using bovine, ovine, hyocholic acid, cholesterol, bilirubin and inorganic salt as raw materials. Because the artificial bezoar is formed by combining several chemical drugs and biological products, the pharmacological difference from the natural bezoar exists, and the whole treatment effect of the traditional Chinese medicine can not be exerted. This has forced the search for methods for producing natural bezoar bovis.

Chinese science and technology workers in the middle and late 70 s successfully cultivated calculus bovis in the gall bladder of live cattle. The Ministry of health is responsible for systematic examination of the ingredient content, pharmacology, health and other aspects of the cultivated bezoar by related research and development units and Chinese medicine and biological article identification institute. The result shows that the cultivated bezoar is completely similar to the natural bezoar. The Ministry of health stipulates quality standards; the cultured calculus bovis contains bilirubin above 35%, and can be used as emergency medicine. In 1991, a new certificate for cultivating calculus bovis was reached under the Ministry of health, and the calculus bovis cultivation in China is transferred from the scientific research stage to the production and development stage.

The technology for increasing the yield of the bezoar by successfully developing the escherichia coli implanted in the gall bladder of the living cattle according to the basic distance of the formation of the natural bezoar by Chinese scientific and technological workers, but as ox bile bacteria increase and change the PH value of the bile, the viscosity of the bile decreases, and the utilization rate of calculus bovis microparticles in the bile is reduced.

Therefore, there is a need to provide a new method for preparing bezoar to solve the above technical problems.

Disclosure of Invention

The invention aims to provide a bile bezoar generator for improving the utilization rate of calculus bovis tiny particles.

The bezoar generator comprises a generating device, a driving device and a recovery device, wherein the generating device comprises a shell, a liner which is arranged at the bottom of the shell and is provided with an accommodating space, and a yellowing bed which is accommodated in the accommodating space, the yellowing bed is in an inverted umbrella-shaped structure, the tip end of the yellowing bed is arranged on one side of the liner, and the driving device comprises a transmission shaft which penetrates through the liner and the end part of the yellowing bed and is in driving connection with the liner.

Preferably, the driving device further comprises a motor, a motor transmission belt pulley sleeved on the motor rotating shaft, a rotating shaft transmission belt pulley sleeved on the transmission shaft, a transmission belt connected with the motor and the transmission shaft in a driving manner through the motor transmission belt pulley and the rotating shaft transmission belt pulley, and a driven shaft arranged at the other end of the inner container and coaxial with the transmission shaft.

Preferably, the recovery device comprises a drainage and exhaust pipe and a bile discharge pipe which are close to the driven shaft and respectively communicated with the inner container.

Preferably, the shell is made of stainless steel materials and is in a flying saucer shape, and the front protrusion is spherical.

Preferably, the inner container is made of non-toxic polyethylene material and is oval.

Preferably, the inner surface of the inner container is provided with a strip-shaped bulge for stirring the bile to do centripetal rotary motion.

Preferably, the yellowing bed is made of non-toxic polyethylene materials, the length of the yellowing bed is the inner container 3/1, the diameter of the yellowing bed is the inner container 3/2, the yellowing bed is open at the upper side, the lower side of the yellowing bed is connected with a bile discharge pipe, and the inner wall of the yellowing bed is lined with low-density woven gauze.

Preferably, the device further comprises a frame, and the frame supports the generating device, the driving device and the recovery device to be suspended on a supporting surface.

The invention also provides a method for preparing bezoar by using the bezoar generator for bezoar with an external gallbladder, which comprises the following steps:

s1, selecting cattle above 4 years old, and inoculating nonpathogenic bile-resistant Escherichia coli in the cattle gallbladder;

s2, inflammation is generated due to bacterial proliferation in the gall bladder of the cattle, tiny bezoar particles appear in the intrahepatic bile duct, stasis is generated in gall bladder of the cattle, mucinous protein is increased, bilirubin calcium salt is complexed to form tiny bezoar particles, and then the gall is drained to the inner bladder through a drainage and exhaust pipe;

s3, starting a cow external liner bezoar generator, driving the liner to rotate axially by a motor, enabling bile to shake and rotate centripetally in the liner, enabling the centripetally flowing in the liner to accelerate sedimentation and adhesion of calculus bovis microparticles and accelerate aggregation of the calculus bovis microparticles;

and S4, the motor is turned off, the inner container stops rotating, and the prepared bile is discharged through the bile discharge pipe, so that the preparation of the bezoar is completed.

Preferably, the non-pathogenic bile-resistant escherichia coli is a. chrysosporium.

Compared with the prior art, the bezoar generating device for the external gallbladder of the cattle, provided by the invention, utilizes the principle of gallbladder hydrodynamics when natural bezoar is generated, and processes the calculus bovis generator for the gallbladder, so that bile is subjected to centripetal rocking and rotating motion in the gallbladder, the aggregation of tiny calculus bovis particles is accelerated, and the output of bezoar is increased.

Drawings

Fig. 1 is a schematic structural diagram of a cow in vitro bile bezoar generator according to a preferred embodiment of the present invention.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Referring to fig. 1, the cow in vitro gallbladder bezoar generator provided by the present invention includes a generating device, a driving device, a recovering device and a frame 12, wherein the frame 12 supports the generating device, the driving device and the recovering device suspended on a supporting surface.

The generating device comprises a shell 1, an inner container 2 which is arranged at the bottom of the shell 1 and is provided with an accommodating space, and a yellowing bed 3 which is accommodated in the accommodating space, wherein the yellowing bed 3 is in an inverted umbrella-shaped structure, the tip end of the yellowing bed is arranged at one side of the inner container 2,

the driving device comprises a transmission shaft 7, a motor 9, a motor transmission belt pulley 10 sleeved on a rotating shaft of the motor 9, a rotating shaft transmission belt pulley 5 sleeved on the transmission shaft 7, a transmission belt 8 connected with the motor 9 and the transmission shaft 7 in a driving mode through the motor transmission belt pulley 10 and the rotating shaft transmission belt pulley 5, and a driven shaft 11 arranged at the other end of the inner container 2 and coaxial with the transmission shaft 7.

The transmission shaft 7 penetrates through the inner container 2, the end parts of the yellowing bed 3 and the inner container 2 to be in driving connection.

The recovery device comprises a drainage and exhaust pipe 13 and a bile discharge pipe 14 which are close to the driven shaft 11 and are respectively communicated with the inner container 2.

The shell 1 is made of stainless steel materials and is in a flying saucer shape, and the front protrusion is spherical.

The inner container 2 is made of nontoxic polyethylene material and is oval.

The inner surface of the inner container 2 is provided with a strip-shaped bulge (not shown) for stirring the bile to do centripetal rotary motion.

In this embodiment, the yellowing bed 3 is made of non-toxic polyethylene material, the length of the yellowing bed is the inner container 3/1, the diameter of the yellowing bed is the inner container 3/2, the yellowing bed is shaped in such a way that the upper side is open, the lower side is connected with a bile discharge pipe 14, and the inner wall of the yellowing bed 3 is lined with low-density woven cotton cloth 4.

The invention also provides a method for preparing bezoar by the bezoar vitro gallbladder bezoar generator, which comprises the following steps:

s1, selecting cattle above 4 years old, and inoculating nonpathogenic bile-resistant Escherichia coli in the cattle gallbladder;

selection of the yellow-planted cattle: selecting cattle with healthy and disease-free age of 4-10 years, preferably selecting culled cattle with poor breeding ability. Individuals within the age range are too small to be yellow-rooted. The variety is selected from cattle, yak and unlimited hair color.

Selecting a breeding environment: the selection sequence is, in turn, mountain-hill-plain.

Feeding mode: the selection sequence is grazing, semi-grazing and barn feeding in turn. During feeding, calculus bovis can be induced by feeding with lithagogue bait. The stone-forming bait is feed containing 1% casein, 1.5% sucrose, 1% lard, 1% cellulose, 0.02% cholic acid, and 0.05% cholesterol. The feeding time of the stone-causing food is between 5 and 7 points per evening. The feed amount of each cattle is 300 to 400 grams.

The nonpathogenic bile-resistant escherichia coli is yellow bacillus.

The yellow-producing strain is selected, i.e. gallbladder infection is the basis for forming bezoar, Escherichia coli is preferably separated from natural bezoar-producing bile of ox and then used as the yellow-producing strain, and β -G bacteria with high enzyme activity are selected as the yellow-producing strain by laboratory determination of Escherichia coli, and mixed serotype strains can also be selected as the yellow-producing bacteria.

Other conditions that improve the yield and quality of bezoar: the oral cholestyramine is administered to cattle during the period of bezoar planting, and the dosage is 20g per day until the end of bezoar planting. Oral cholestyramine can promote the excretion of bile salt from feces, reduce the storage amount of cholic acid, increase the content of cholesterol, and thus promote the increase of the yield of bezoar. During the yellowing, the simulated gall bladder is injected with the Gantaile and the calcium chloride injection, and the Gantaile is 0.5g/dl once a day. Ca2+5 ng/dl. The injection sequence is that firstly the glucurolactone is injected and then the calcium chloride is injected, which are separated by 4 hours. Daily injection is carried out until the end of yellowing.

S2, inflammation is generated due to bacterial proliferation in the gall bladder of the cattle, tiny bezoar particles appear in the intrahepatic bile duct, stasis is generated in gall bladder of the cattle, mucinous protein is increased, bilirubin calcium salt is complexed to form tiny bezoar particles, and then the gall is drained to the inner bladder through a drainage and exhaust pipe;

s3, starting a cow external liner bezoar generator, driving the liner to rotate axially by a motor, enabling bile to shake and rotate centripetally in the liner, enabling the centripetally flowing in the liner to accelerate sedimentation and adhesion of calculus bovis microparticles and accelerate aggregation of the calculus bovis microparticles;

and S4, the motor is turned off, the inner container stops rotating, and the prepared bile is discharged through the bile discharge pipe, so that the preparation of the bezoar is completed.

The method for cultivating calculus bovis can effectively improve the yield and quality of calculus bovis, shorten cultivation time and improve yield. The calculus bovis is produced by 19.8 plus or minus 1.3g in each time, and the cultivation time is 138 plus or minus 12 days. The bezoar bilirubin content is 35.86% + -4.32%, wherein 68.3% of the samples have bilirubin content up to or exceeding 35%.

The invention can provide guarantee for cattle raising enterprises to resist market risk, and can cultivate the bezoar during raising period to improve the output value of cattle. On average, each cow can increase at least 10000-. Provides guarantee for reducing the breeding risk and providing high-quality medicinal bezoar.

The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.

Claims (10)

1. An external bile bezoar generator for cattle, comprising: the device comprises a generation device, a driving device and a recovery device, wherein the generation device comprises a shell, an inner container which is arranged at the bottom of the shell and is provided with an accommodating space, and a yellow bed which is accommodated in the accommodating space, the yellow bed is of an inverted umbrella-shaped structure, the tip end of the yellow bed is arranged on one side of the inner container, the driving device comprises a transmission shaft, and the transmission shaft penetrates through the inner container, the end part of the yellow bed and the inner container to be in driving connection.

2. The bezoar generator according to claim 1, wherein the driving device further comprises a motor, a sleeve disposed on a motor transmission belt pulley of the motor rotation shaft, a sleeve disposed on a rotation shaft transmission belt pulley of the transmission shaft, a driven shaft connected with the motor and the transmission belt of the transmission shaft through the motor transmission belt pulley and the rotation shaft transmission belt pulley in a driving manner, and a driven shaft disposed at the other end of the inner container and coaxial with the transmission shaft.

3. The bezoar generator according to claim 1, wherein the recovery device comprises a drainage and exhaust tube and a bile discharge tube adjacent to the driven shaft and respectively communicating with the inner bladder.

4. The bezoar generator according to claim 1, wherein the housing is made of stainless steel and is made into a flying saucer shape, and the front surface of the housing is convex and spherical.

5. The bezoar generator according to claim 1, wherein the inner container is made of non-toxic polyethylene material and is oval.

6. The bezoar generator according to claim 1, wherein the inner surface of the inner container has elongated protrusions for stirring bile to perform centripetal rotation.

7. The bezoar generator according to claim 1, wherein the yellowing bed is made of non-toxic polyethylene material, the length of the yellowing bed is 3/1, the diameter of the yellowing bed is 3/2, the yellowing bed is shaped with an open upper side and a bile drainage tube connected to the lower side, and the inner wall of the yellowing bed is lined with low-density woven cotton cloth.

8. The bovine in vitro bile bezoar generator according to claim 1, further comprising a frame supporting the generating means, the driving means and the recovery means suspended from a support surface.

9. A method for preparing bezoar by using the bezoar generator for bezoar with an external gallbladder of a cow is characterized by comprising the following steps:

s1, selecting cattle above 4 years old, and inoculating nonpathogenic bile-resistant Escherichia coli in the cattle gallbladder;

s2, inflammation is generated due to bacterial proliferation in the gall bladder of the cattle, tiny bezoar particles appear in the intrahepatic bile duct, stasis is generated in gall bladder of the cattle, mucinous protein is increased, bilirubin calcium salt is complexed to form tiny bezoar particles, and then the gall is drained to the inner bladder through a drainage and exhaust pipe;

s3, starting a cow external liner bezoar generator, driving the liner to rotate axially by a motor, enabling bile to shake and rotate centripetally in the liner, enabling the centripetally flowing in the liner to accelerate sedimentation and adhesion of calculus bovis microparticles and accelerate aggregation of the calculus bovis microparticles;

and S4, the motor is turned off, the inner container stops rotating, and the prepared bile is discharged through the bile discharge pipe, so that the preparation of the bezoar is completed.

10. The method of producing bezoar according to claim 9, wherein the non-pathogenic bile-resistant escherichia coli is flavobacterium sp.

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