CN105821017B - Corn pyrophosphatase gene ZmPPase4 is improving the application in stress resistance of plant - Google Patents
Corn pyrophosphatase gene ZmPPase4 is improving the application in stress resistance of plant Download PDFInfo
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Abstract
The present invention relates to corn pyrophosphatase gene ZmPPase4 to improve the application in stress resistance of plant, and the expression vector containing corn ZmPPase4 gene is transferred in plant, filters out transgenic plant, and carried out Salt-Tolerance Identification.ZmPPase4 gene of the invention can significantly improve the Saline alkali tolerance of plant, and the transgenic crop for cultivating high yield is of great significance.
Description
Technical field
The present invention relates to genetic engineering fields, specifically, being related to corn pyrophosphatase gene ZmPPase4 is improving plant
Application in object resistance.
Background technique
Saline Alkali Stress is an important factor for influencing production estimation.Plant is led in the soil containing high salt concentration ion
Water Transportation is crossed, will lead to plant tissue ion accumulation.Excessive soluble ion includes sodium ion, and potassium ion etc. will affect
Plant normal development.In addition, ionic stress caused by the salt of high concentration can also cause osmotic stress, and then seriously affect plant production
Amount.Plant can balance salt ion absorption in several ways, including limitation intake, the excretion for increasing sodium ion, by salt ion
Compartmentation to vacuole, and control sodium ion long-distance transport from root to aerial part, to weaken aerial part cytoplasm
Middle sodium content.
Pyrophosphatase (pyrophosphatase, PPase, EC3.6.1.1) be it is a kind of be widely present in nature can
Participate in the hydrolase of a variety of metabolic responses of synthesis sugar and albumen.Pyrophosphatase hydrolyzes pyrophosphoric acid group using pyrophosphoric acid as substrate
For phosphate.In general, pyrophosphatase can be divided into two major classes: one kind is the soluble nothing being present in cytoplasm and cellular matrix
Machine pyrophosphatase；Another is the insoluble enzyme in conjunction with film.PPase activity is higher in cytoplasm, by not cutting off the water supply
Solution PPi makes PPi in cytoplasm keep reduced levels.PPase and vacuole ATPase collective effect on vegetation water vacuolar membrane are constituted and are planted
The complete proton pump transdermal delivery system of object, plays an important role in Physiological Activities of Plants.In addition, PPase may also participate in by
Inorganic ion transports vacuole, to adjust cell turgor.
More early for the Physiological Mechanism of inorganic pyrophosphatase, Kawlsson in 1975 is from beet (Beta Vugaris)
The PPase of activation potassium ion is separated in root for the first time, it is while hydrolyzing PPi, moreover it is possible to by H+Such as vacuole is transported from cytoplasm
In film.Nineteen ninety, Kieber have cloned the pyrophosphatase that an ORF is 789bp from arabidopsis, and molecular weight of albumen is about
30kDa.Nineteen ninety-five, Jorge have cloned an inorganic pyrophosphatase from potato, have found the catalyst structure domain of the protein sequence
It is very high with arabidopsis homeodomain similarity, from chadogram and conserved structure analysis shows potato, arabidopsis and Escherichia coli
Inorganic pyrophosphate hdac protein structure in all contain a uptight hydrophobin domain.2008, Zhang Ning, Si Huai army etc.
People has cloned a potato PPase gene, by biological software to the analytical table of the protein of PPase gene and its coding
Bright, the homology of the gene and nightshade plant is up to 89%~97%, with the homologys of other species genes 77%~
Between 80%.Its protein encoded has multiple phosphorylation sites, without transmembrane region.By subcellular localization it is found that it leads
It is positioned at cytoplasm and mitochondrial matrix, important regulating and controlling effect may be played in glycometabolism.Zhang Chunfeng in 2009, department bosom
Army etc. has carried out functional study to the potato plant of become a full member justice and antisense PPase gene, and the activity for analyzing pyrophosphatase is surveyed
Fixed condition, the results showed that PPase activity with higher when pH value is 8.0, the optimum temperature of enzymatic reaction is 42 DEG C, different
Mg in cation activity detection2+With PPase affinity highest.
Inorganic pyrophosphatase will be later than to the research of vacuolar pyrophosphatase (V-PPase), 1992, the head such as Sarafian K
Secondary screening and cloning goes out arabidopsis V-PPase gene, and result of study shows that the code area arabidopsis V-PPase is 2310bp, coding
769 amino acid.2000, it is cloned into the vacuolar pyrophosphatase of the 2nd seed type in arabidopsis, is named as AVP2.Upper
On the basis of stating research, researcher is successfully cloned into coke according to the conserved sequence design primer of V-PPase from various plants
Phosphatase gene sequence is compared by the cDNA sequence between several species it is found that its nucleic acid sequence is very conservative, in its enzymatic activity
Similitude can reach nearly 90% in conserved sequence structural domain.H+Transport pyrophosphorylase (H+- PPase) belong to vacuolar pyrophosphatase
One kind, participate in Adversity-stressed Plant response in play an important role.Arabidopsis AVP1 encodes a H+- PPase pyrophosphatase,
H is mainly played on tonoplast+Transhipment effect, by transporting H+Tonoplast is set to generate H+Gradient, to drive Na+/H+Ionic pump etc.
Antiport.AVP1 gene is overexpressed in arabidopsis can significantly improve that plant is drought-enduring and salt tolerant alkali ability.Thus illustrate H+-
PPase passes through regulation H under Adversity-stressed Plant+Gradient has important meaning to plant stress-resistance so as to adjust intraor extracellular pressure difference
Justice.
As it can be seen that being at present hot fields in recent years for the research of plant stress-resistance gene, currently, the research work of this respect
Make to be substantially to carry out on model plant arabidopsis.For this genoid in important crops (such as rice, corn, wheat)
Research it is less, more particularly to the functional study of corn pyrophosphatase gene, there is not been reported.
Summary of the invention
The object of the present invention is to provide corn pyrophosphatase gene ZmPPase4 to improve the application in stress resistance of plant.
In order to achieve the object of the present invention, corn pyrophosphatase gene ZmPPase4 provided by the invention is improving plant stress-resistance
Property in application, wherein gene ZmPPase4 encode albumen amino acid sequence is as shown in SEQ ID No.1 or the sequence is through replacing
Change, lack or add one or several amino acids formed amino acid sequences with same function.
Resistance of the present invention includes Saline alkali tolerance, i.e., to the resistance of Saline Alkali Stress.
Plant of the present invention includes but is not limited to arabidopsis.
Application above-mentioned, by being overexpressed the CDS sequence of gene ZmPPase4 in plant, to improve transgenic plant
Resistance.The specific method is as follows:
Corn total serum IgE is extracted, reverse transcription designs pcr amplification primer according to the sequence of target gene ZmPPase4 at cDNA
Object, amplifies the CDS sequence of the gene ZmPPase4 as shown in SEQ ID No.2 from corn cDNA, and by the CDS sequence structure
It is built on plant expression vector, converts plant, screening transgenic plant.
Preferably, by Gateway technology by the CDS sequence construct to plant expression vector, the specific method is as follows:
The CDS sequence is connected on entry vector pGWC, obtained recombinant vector pGWC-ZmPPase4 and carrier
PEarleyGate202 carries out LR reaction to get recombinant expression carrier pEarleyGate202-ZmPPase4, is used in combination
PEarleyGate202-ZmPPase4 converts plant (Fig. 1).
PCR amplification the primer is (SEQ ID No.3 and 4):
Forward primer F 5'-ATGGCGCCCGCTGTAGAAG-3'
Reverse primer R 5'-CTACCTCCTCAGGCCCTCAAC-3'
The expression vector for carrying target gene can be by using Ti-plasmids, plant viral vector, directly delivered DNA, micro-
The standard biologics technical method such as injection, electroporation imports (Weissbach, 1998, Method for Plant in plant cell
Molecular Biology VIII, Academy Press, New York, the 411-463 pages；Geiserson and Corey,
1998, Plant Molecular Biology, 2nd Edition)。
Present invention preferably employs the methods of mediated by agriculture bacillus to convert plant.
Preferably, glufosinate-ammonium (phosphinothricin, PPT) screening transgenic plant is utilized.
The present invention further provides corn pyrophosphatase gene ZmPPase4, the CDS sequences of gene ZmPPase4 are as follows:
I) nucleotide sequence shown in SEQ ID No.2；Or
Ii) nucleotide sequence shown in SEQ ID No.2 be substituted, lack and/or increase one or more nucleotide and
Express the nucleotide sequence of identical function protein；Or
Iii) hybridize under strict conditions with sequence shown in SEQ ID No.2 and express the nucleotide of identical function protein
Sequence, the stringent condition are in 0.1 × SSPE containing 0.1%SDS or 0.1 × SSC solution containing 0.1%SDS, at 65 DEG C
Lower hybridization, and film is washed with the solution；Or
Iv) and i), ii) or nucleotide sequence iii) there is 90% or more homology and express identical function protein
Nucleotide sequence.
The present invention relates to corn pyrophosphatase gene ZmPPase4 to improve the application in stress resistance of plant, will contain corn
The expression vector of ZmPPase4 gene is transferred in plant, filters out transgenic plant, and carried out Salt-Tolerance Identification.Of the invention
ZmPPase4 gene participates in plant salt tolerance alkali process by regulation downstream related gene, for cultivating the transgenic crop of high yield
It is of great significance.
Detailed description of the invention
Fig. 1 is the flow chart that recombinant expression carrier pEarleyGate202-ZmPPase4 is constructed in the embodiment of the present invention 1.
Fig. 2 is the PCR testing result of 3 transgenic strain target gene of the embodiment of the present invention；Wherein, M:Marker；1:
Positive control (using carrier pEarleyGate202-ZmPPase4 as pcr template)；2: negative control (is with wildtype Arabidopsis thaliana DNA
Pcr template)；13,19,21,27,42,43,44,45,48,50,60 with 61:ZmPPase4 difference transgenic line target gene
Amplification.
Fig. 3 is 4 transgenic arabidopsis of the embodiment of the present invention and WT lines in the culture containing various concentration NaCl
Growing state comparison result on base.Turn the arabidopsis seed program request of 35S::ZmPPase4 gene in the MS training containing 150mM NaCl
It supports on base, after 4 DEG C of vernalization 3d, is placed in 22 DEG C of cultures, observation upgrowth situation transgenic line ties up to the plate of 150mM NaCl after 10d
Upper growth is obviously suppressed, and transgenic line OE-21, OE-37, OE-42 growing way is substantially better than WT, and NaCl is to seedling root long
There is obvious inhibiting effect.
Fig. 4 is the phenotype comparison result of high-salt stress Transfer-gen plant and WT lines in the embodiment of the present invention 5；Its
In, A is root long comparison result, and B is fresh weight comparison result.
Specific embodiment
The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention..Unless otherwise specified, embodiment
According to conventional laboratory conditions, such as Sambrook molecular cloning experiment handbook (Sambrook J&Russell DW,
Molecular cloning:a laboratory manual, 2001), or according to the condition of manufacturer's specification suggestion.
The clone of 1 corn pyrophosphatase gene ZmPPase4 of embodiment and the building of plant expression vector
1, corn total serum IgE is extracted, reverse transcription designs pcr amplification primer according to the sequence of target gene ZmPPase4 at cDNA
Object (SEQ ID No.3 and 4), the CDS sequence (SEQ ID No.2) of gene ZmPPase4 is amplified from corn cDNA, and uses fine jade
Sepharose kit recycles target fragment.
2, the segment of recycling is connect with pGWC carrier, then converts DH5 α competent cell afterwards, obtain positive monoclonal,
Through sequencing identification obtain with the identical monoclonal of objective gene sequence, shake bacterium and extract the monoclonal plasmid, be named as
pGWC-ZmPPase4。
3, using pGWC-ZmPPase4 as entry vector, pEarleyGate202 is expression vector, is reacted by LR, is obtained
Recombinant expression carrier pEarleyGate202-ZmPPase4 (Fig. 1, i.e. overexpression vector 35S::ZmPPase4).
The acquisition of 2 transgenic Arabidopsis plants of embodiment
1, the overexpression vector 35S::ZmPPase4 built is transferred to Agrobacterium GV3101.
2, wildtype Arabidopsis thaliana is converted with the method for being stained with flower dip dyeing (floral dipping).
3, then the T0 obtained after conversion was directly seeded into Nutrition Soil and is grown for seed vernalization 3 days, normal growth is about
After two weeks, transformed plant is screened for arabidopsis with 0.5 ‰ glufosinate-ammonium sprinkling T0.
4, due to having the Bar gene of glufosinate-resistant in conversion overexpression vector used, when conversion, can be with target gene
It is transferred to plant together, therefore sprays most of unconverted plant death after PPT, and transformed plant still is able to normal growth.
Transformed plant harvests T1 for seed according to different strains respectively.
5, it after harvesting each transgenic line T2 for seed, is carried out disinfection processing with 0.5% NaClO to seed.Then point
Kind in the MS culture medium flat plate containing 0.5 ‰ PPT (each transgenic line needs about 45 seeds), it is negative pair with wild type
According to.
6, it is placed in 22 DEG C of illumination boxs within vernalization three days and grows, observe growth of seedling situation after a week.Wild type exists
It can not survive in MS culture medium containing 0.5 ‰ PPT；T2 is for heterozygote transgenic line due to that can send out during generating filial generation
Raw Gene Isolation and independent assortment, therefore understand some seed without glufosinate resistance and occur；Only T2 turns on behalf of homozygote
The seed of gene strain can all survive in the MS culture medium containing PPT.
The identification of 3 transgenic positive strain of embodiment
1, using the total DNA of the positive plant of extraction as template, PCR expansion is carried out with the primer as shown in SEQ ID No.4 and 5
Increase, positive plant can amplify the band of 645bp, and false positive plant is then without pcr amplification product.WT lines cannot expand
Increase purpose band out.(Fig. 2)
2, it after harvesting each transgenic line T2 for seed, is carried out disinfection processing with 0.5% NaClO to seed.Then point
Kind in the MS culture medium flat plate containing 0.5 ‰ PPT (each transgenic line needs about 45 seeds), it is negative pair with wild type
According to.
3, it is placed in 22 DEG C of illumination boxs within vernalization three days and grows, observe growth of seedling situation after a week.Wild type exists
It can not survive in MS culture medium containing 0.5 ‰ PPT.
4, T2 for heterozygote transgenic line due to can occur during generating filial generation Gene Isolation and independent assortment,
Therefore understand some seed without kalamycin resistance to occur；Only T2 can be all on behalf of the seed of homozygote transgenic line
It survives in the MS culture medium containing kanamycins.
4 transgenic plant of embodiment and the resistance of WT lines compare
It will turn the arabidopsis strain program request of 35S::ZmPPase4 gene to put down in the MS of the NaCl containing 0mM, 125mM, 150mM
On plate, 4 DEG C after vernalization 3 days, it is placed in 22 DEG C, in the dark incubator in 16 hours illumination/8 hour, phenotype is observed after 5 days and is carried out
Analysis.As a result see Fig. 3, from the results, it was seen that wildtype Arabidopsis thaliana leaf wilting yellowing under high-salt stress is serious, and
Transgenic line OE-21, OE-37, OE-42 growing way is preferable.Transgenic line and wild type in normal MS culture medium phenotype without
Significant difference.
5 high-salt stress Transfer-gen plant of embodiment and the phenotype of WT lines compare
It will turn 35S::ZmPPase4 gene arabidopsis T3 homozygous lines (OE-21, OE-37, OE-42) and wild type (WT)
Arabidopsis seed, after sterilizing respectively point be sowed at containing 125mM, 150mM NaCl MS solid medium on, 4 DEG C vernalization 3 days, will
Culture dish is placed in culturing room, vertically cultivates under 16h light/8h dark condition, be overexpressed gene ZmPPase4 plant containing 125mM,
After being cultivated 14 days on the MS culture medium of 150mM NaCl.The root long and fresh weight of different plants, each transgenic line are measured respectively
10 plants of measurement, 3 repetitions.As a result Fig. 4 is seen, from the results, it was seen that turning 35S::ZmPPase4 gene arabidopsis 125mM's
In NaCl culture medium, WT root long only has 3-4cm, and the transgenic line root long for being overexpressed gene ZmPPase4 is 6cm, more highly concentrated
It spends under the conditions of the NaCl of (150mM), WT root long only has 2.5cm, wild type WT to occur on the MS culture medium of the NaCl of 150mM white
Change and wilty phenotype.Transgenic line and WT lines no significant difference on normal MS culture medium.Equally, by 125mM,
Transgenic line fresh weight has significant difference compared with WT after 150mM NaCl Stress treatment, illustrates that high concentration NaCl stress can be with
It is serious to inhibit plant strain growth, and transgenic line has apparent salt tolerance.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.
Claims (7)
1. corn pyrophosphatase gene ZmPPase4 is improving the application in stress resistance of plant, wherein gene ZmPPase4 encodes egg
White amino acid sequence is as shown in SEQ ID No.1；
The resistance is Saline alkali tolerance；The plant is arabidopsis, corn.
2. application according to claim 1, which is characterized in that by the CDS for being overexpressed gene ZmPPase4 in plant
Sequence, to improve transgenic plant resistance.
3. application according to claim 2, which is characterized in that extract corn total serum IgE, reverse transcription is at cDNA, according to purpose
The sequence of gene ZmPPase4 designs PCR amplification primer, amplifies the gene as shown in SEQ ID No.2 from corn cDNA
The CDS sequence of ZmPPase4, and plant on the CDS sequence construct to plant expression vector, will be converted, screening transgenic is planted
Strain.
4. application according to claim 3, which is characterized in that by Gateway technology by the CDS sequence construct to plant
On object expression vector, the specific method is as follows: the CDS sequence being connected on entry vector pGWC, obtained recombinant vector
PGWC-ZmPPase4 carries out LR with carrier pEarleyGate202 and reacts to get recombinant expression carrier pEarleyGate202-
ZmPPase4, and plant is converted with pEarleyGate202-ZmPPase4.
5. application according to claim 3, which is characterized in that PCR amplification the primer are as follows:
Forward primer F 5'-ATGGCGCCCGCTGTAGAAG-3'
Reverse primer R 5'-CTACCTCCTCAGGCCCTCAAC-3'.
6. according to the described in any item applications of claim 3-5, which is characterized in that converted and planted using the method for mediated by agriculture bacillus
Object.
7. according to the described in any item applications of claim 3-5, which is characterized in that utilize glufosinate-ammonium screening transgenic plant.
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| CN113481180A (en) * | 2021-07-05 | 2021-10-08 | 吉林大学 | Alkaline thermophilic inorganic pyrophosphatase and application thereof in enhancing polymerase chain reaction and UDP-galactose synthesis reaction |
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