CN105794849A - Efficient plant growth regulation composition containing harpin protein - Google Patents
Efficient plant growth regulation composition containing harpin protein Download PDFInfo
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- CN105794849A CN105794849A CN201510001025.8A CN201510001025A CN105794849A CN 105794849 A CN105794849 A CN 105794849A CN 201510001025 A CN201510001025 A CN 201510001025A CN 105794849 A CN105794849 A CN 105794849A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention relates to an efficient plant growth regulation composition containing harpin protein. The plant growth regulation composition contains effective active ingredients, i.e., an active ingredient A and an active ingredient B, wherein the active ingredient A is the harpin protein, and the active ingredient B is any selected from the following compounds: Forchlorfenuron, Cycocel and choline chloride. The plant growth regulation composition is mainly used for enhancing the activity of enzymes, prompting the germination of seeds, increasing germination percentage, enhancing photosynthetic intensity, increasing chlorophyll content, keeping green and preventing aging, promoting the development of root systems, promoting the real fertilizer absorption of crops, assisting the good growth of inferior parts of the crops, enhancing the water and fertilizer absorption of plants and regulating the body water balance of the plants, so that the drought resistance and cold resistance of the plants are improved; and the plant growth regulation composition has the functions of improving immunity, enhancing the resistance to diseases and pests, improving crop quality and increasing both production and income.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of efficient plant growth containing super quick albumen and regulate the compositions application crops.
Technical background
Super quick albumen (harpinprotein): super quick albumen belongs to native protein, has growth regulation effect and strengthens the disease resisting effect of plant Nicotiana tabacum L..
CPPU, chlorocholine chloride, choline chloride belong to conventional plant growth regulator, are widely used in regulating the growth of plant.
For target plant, plant growth regulator is the non-nutritive chemical substance of external source, generally can conduct in plant to site of action, just can promote or suppress some link of its life process with very low concentration, so as to the needs meeting the mankind.Every plant growth regulators has specific purposes, and application technology requires quite strict, and target plant only could produce specific effect under specific application conditions (including extraneous factor).Often change concentration and will obtain contrary result, for instance have facilitation at low concentrations, then become inhibitory action in higher concentrations.Single plant growth regulator is also comparatively single to the promotion growth of crop, and the plant growth regulator of the different mechanisms of action is composite can effectively solve this problem.The application active components A is different from the active component B mechanism of action, and mutually composite have good potentiation within the specific limits, and about super quick albumen and CPPU, chlorocholine chloride, choline chloride binary built, at home and abroad there is not yet relevant report at present.
Summary of the invention
The purpose of the present invention is to propose to and a kind of there is the plant growth regualting composition containing super quick albumen that synergistic function, use cost are low, effective.
The present invention is achieved through the following technical solutions:
A kind of efficient plant growth containing super quick albumen regulates compositions, effective active composition is active components A and active component B, it is characterized in that: the weight ratio of active components A and active component B is 80 1～1 100, described active components A is super quick albumen, active component B one in CPPU, chlorocholine chloride, choline chloride, active components A and the preferred weight ratio of active component B are 60 1～1 80；The weight ratio being more preferably super quick albumen and CPPU is 30 1～1 25, and the weight ratio of super quick albumen and chlorocholine chloride is 20 1～1 40, and the weight ratio of super quick albumen and choline chloride is 25 1～1 35.
Described plant growth regulator composition, wherein effective active component content accounts for the 0.1%～70% of gross weight, it is preferred to 1%～50%.
Surfactant selected by the plant growth regualting composition of the present invention is known in those skilled in the art: can be selected from one or more in dispersant, wetting agent, emulsifying agent, thickening agent or defoamer.According to different dosage form, can also containing stabilizer known in those skilled in the art, antifreeze etc. in preparation.
The plant growth regualting composition of the present invention is for promoting the growth of crops, and described crops include cereal crops, legume crop, fibre crops, sugar crop, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop.
The plant growth regualting composition of the present invention, it is possible to be processed on any pesticide acceptable dosage form on demand.Wherein preferred dosage form is wettable powder, water dispersible granules, aqueous emulsion, microemulsion, microcapsule suspending agent, water preparation, soluble liquid, soluble powder, it is also possible to make seed coat agent, suspending agent, suspension emulsion, microcapsule suspension-suspendinagent agent, effervescent, ultra low volume liquids.
Compositions comprises following constituent content when making wettable powder: active components A 0.1%～20%, active component B0.1%～70%, dispersant 2%～10%, wetting agent 2%～10%, filler surplus.
Compositions includes following constituent content when making water dispersible granules: active components A 0.1%～20%, active component B0.1%～70%, dispersant 3%～12%, wetting agent 1%～8%, disintegrating agent 1%～10%, binding agent 0～8%, filler surplus.
Compositions comprises following constituent content when making aqueous emulsion: active components A 0.1%～20%, active component B0.1%～50%, solvent 1%～30%, emulsifying agent 1%～15%, antifreeze 0～8%, thickening agent 0～2%, defoamer 0.01%～2%, deionized water add to 100%.
Compositions comprises following constituent content when making microemulsion: active components A 0.1%～20%, active component B0.1%～50%, solvent 1%～20%, emulsifying agent 3%～25%, antifreeze 0～8%, defoamer 0.01%～2%, deionized water add to 100%.
Compositions includes following constituent content when making microcapsule suspending agent: active components A 0.1%～20%, active component B0.1%～50%, macromolecule cyst material 2%～10%, dispersant 1%～10%, solvent 1%～10%, emulsifying agent 1%～7%, defoamer 0.01%～2%, pH adjusting agent 0.01%～5%, deionized water add to 100%.
Compositions includes following constituent content when making water preparation: active components A 0.1%～20%, active component B0.1%～50%, cosolvent 2%～6%, wetting agent 1%-10%, antifreeze 0～8%, deionized water add to 100%.
Compositions includes following constituent content when making soluble liquid: active components A 0.1%～20%, active component B0.1%～50%, emulsifying agent 1%～10%, cosolvent 2%～6%, solvent add to 100%.
Compositions includes following constituent content when making soluble powder: active components A 0.1%～20%, active component B0.1%～50%, dispersant 3～12%, wetting agent 1～8%, disintegrating agent 1～10%, filler 10～93%.
The wettable powder the key technical indexes of the present invention:
The water dispersible granules the key technical indexes of the present invention:
The aqueous emulsion the key technical indexes of the present invention:
The microemulsion the key technical indexes of the present invention:
The microcapsule suspending agent the key technical indexes of the present invention:
The water preparation the key technical indexes of the present invention:
The soluble liquid the key technical indexes of the present invention:
Soluble powder the key technical indexes of the present invention:
It is an advantage of the current invention that:
(1) super quick albumen and CPPU, chlorocholine chloride, choline chloride composite after, there is obvious potentiation；(2) activity of enzyme is enhanced, promote germination, improve germination percentage, strengthen rate of photosynthisis, improve chlorophyll content, protect green anti-old, promote well developed root system, promote the crop effective absorption to fertilizer, supplemental crop inferior position part well grows, strengthening plant to water, fertile absorption, regulating the balance of plant internal water, thus improving the drought resisting of plant, winter resistance；(3) there is raising immunity, strengthen disease and insect resistance, improve crop quality, increasing both production and income function.
Detailed description of the invention
Below in conjunction with embodiment to further instruction of the present invention, the percentage ratio in embodiment is all weight percentage, but the invention is not limited in this.
Application Example one
Embodiment 1～9 wettable powder
By super quick albumen, active component B, dispersant, wetting agent, filler mix homogeneously in mixed cylinder, remix uniformly after jet mill is pulverized, can be prepared by wettable powder product of the present invention, be specifically shown in table 1.
Table 1 embodiment 1～9 each component and weight portion
Embodiment 10～18 water dispersible granules
Super quick albumen, active component B, dispersant, wetting agent, disintegrating agent, filler etc. are obtained through comminution by gas stream together the particle diameter needed, adds other auxiliary agents such as binding agent (can add and can be not added with), obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through granulating and dried, can be prepared by water dispersible granules product of the present invention, be specifically shown in table 2.
Table 2 embodiment 10～18 each component and weight portion
Embodiment 19～24 aqueous emulsion
Super quick albumen, active component B, solvent, emulsifying agent are added together, make to be dissolved into homogeneous oil phase；Deionized water, antifreeze (can add and can be not added with), thickening agent (can add and can be not added with), defoamer are mixed, becomes homogeneous aqueous phase.Under high velocity agitation, aqueous phase adding oil phase, surplus deionized water is supplied；Can be prepared by aqueous emulsion product of the present invention, be specifically shown in table 3.
Table 3 embodiment 19～24 each component and weight portion
CPPU, chlorocholine chloride, choline chloride in table 1～3 are exchanged, novel formulation can be prepared.
Embodiment 25～27 microemulsion
Super quick albumen, active component B are dissolved in equipped with in the homogenizer of solvent, emulsifying agent, antifreeze (can add and can be not added with), defoamer are joined in the homogenizer equipped with above-mentioned solution, surplus deionized water strongly mixes after supplying and homogenizes, and finally obtains the microemulsion product of the present invention that clear appearance is transparent.It is specifically shown in table 4.
Table 4 embodiment 25～27 component and content
Embodiment 28,29 microcapsule suspending agent
By super quick albumen, active component B, macromolecule cyst material, solvent mixing, make to be dissolved into homogeneous oil phase, in a shear condition, oil phase is joined containing emulsifying agent, pH adjusting agent, dispersant, defoamer aqueous phase solution in, surplus deionized water is supplied, and bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, make the finely disseminated microcapsule suspending agent product of the present composition, be specifically shown in table 5.
Table 5 embodiment 28,29 component and content
Embodiment 30～38 water preparation
Super quick albumen, active component B, wetting agent, antifreeze, solvent, deionized water etc. are mixed together, prepare the aqua product of the present composition, be specifically shown in table 6.
Table 6 embodiment 30～38 each component and weight portion
Embodiment 39～47 soluble liquid
By emulsifying agent, cosolvent through high speed shear mix homogeneously, add the quick albumen of the excess of imports, active component B, ball milling 2～3 hours in ball mill, make diameter of particle all below 5 μm, surplus solvent is supplied, and can be prepared by soluble liquid product of the present invention, is specifically shown in table 7.
Table 7 embodiment 39～47 each component and weight portion
Embodiment 48～56 soluble powder
The processing technique conventional by super for active component quick albumen, active component B, dispersant, wetting agent, disintegrating agent, filler etc. together drying, weighing, pulverizing, stirring, mixing etc., prepares the soluble powder product of the present composition, is specifically shown in table 8.
Table 8 embodiment 48～56 each component and weight portion
CPPU, chlorocholine chloride, choline chloride, paclobutrazol in table 6～8 are exchanged, novel formulation can be prepared.
This measuring adopts growth of seedling method.Determination step is as follows:
Select in the culture dish that seed of the same size dials in the 9cm size being covered with two layers of filter paper respectively, 10, every ware, add the medicinal liquid 1.5ml configured, comparison adds distilled water, repeats 4 times.Covering ware lid, the artificial climate being placed into 28 ± 1 DEG C is indoor, illumination 10 hours.Height of seedling and root length is checked in 6 days.
Result statistical analysis:
Application Example two:
For studying thing: Fructus Cucumidis sativi
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN: determine the promotion concentration range of super quick albumen and the former medicine of CPPU and the two different ratio mixture through pilot study.
Result of the test:
The mixed liquid of table 9 super quick albumen and CPPU and different proportion thereof the growth-promoting effect to Fructus Cucumidis sativi
nullAfter super quick albumen and CPPU mixing use as can be seen from Table 9，Seedling and root growth can be simultaneously facilitated，Super quick albumen and CPPU proportioning in the scope of 80 1～1 100，All there is stronger potentiation，Especially when the proportioning of super quick albumen and CPPU is when 1:25～30:1，Potentiation becomes apparent from highlighting，Test through applicant，The optimum ratio finding super quick albumen and CPPU is 1:25、1:20、1:15、1:14、1:13、1:12、1:11、1:10、1:9、1:8、1:7、1:6、1:5、1:4、1:3、1:2、1:1、2:1、3:1、4:1、5:1、6:1、7:1、8:1、9:1、10:1、11:1、12:1、13:1、14:1、15:1、20:1、25:1、30:1，Wherein best for 21 effects with super quick albumen and CPPU.
Application Example three:
For studying thing: Fructus Lycopersici esculenti
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN: determine the promotion concentration range of super quick albumen and the former medicine of chlorocholine chloride and the two different ratio mixture through pilot study.
Result of the test:
The mixed liquid of table 10 super quick albumen and chlorocholine chloride and different proportion thereof the growth-promoting effect to Fructus Lycopersici esculenti
After super quick albumen and chlorocholine chloride mixing use as can be seen from Table 10, Seedling and root growth, super quick albumen and chlorocholine chloride proportioning in the scope of 80 1～1 100 can be simultaneously facilitated, all have stronger potentiation.Especially when the proportioning of super quick albumen and chlorocholine chloride is when 1 40～20 1, potentiation becomes apparent from highlighting, test through applicant, the optimum ratio finding super quick albumen and chlorocholine chloride is 1:40,1:35,1:30,1:25,1:20,1:19,1:18,1:17,1:16,1:15,1:14,1:13,1:12,1:11,1:10,1:9,1:8,1:7,1:6,1:5,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,9:1,10:1,15:1,20:1, wherein best for 19 effects with super quick albumen and chlorocholine chloride.
Application Example four:
For studying thing: Oryza sativa L.
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
EXPERIMENTAL DESIGN: determine the promotion concentration range of super quick albumen and the former medicine of choline chloride and the two different ratio mixture through pilot study.
Result of the test:
The mixed liquid of table 11 super quick albumen and choline chloride and different proportion thereof the growth-promoting effect to Oryza sativa L.
After super quick albumen and choline chloride mixing use as can be seen from Table 11, Seedling and root growth, super quick albumen and choline chloride proportioning in the scope of 80 1～1 100 can be simultaneously facilitated, all have stronger potentiation.Especially when the proportioning of super quick albumen and choline chloride is when 1 35～25 1, potentiation becomes apparent from highlighting, test through applicant, the optimum ratio finding super quick albumen and choline chloride is 1:35,1:30,1:25,1:20,1:15,1:14,1:13,1:12,1:11,1:10,1:9,1:8,1:7,1:6,1:5,1:4,1:3,1:2,1:1,2:1,3:1,4:1,5:1,6:1,7:1,8:1,10:1,15:1,20:1,25:1, wherein best for 14 effects with super quick albumen and choline chloride.
Effect experiment part: test medicine is researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provided, comparison medicament 3% super quick protein particle agent (autogamy), 0.1% CPPU soluble concentrate (autogamy), 50% chlorocholine chloride water preparation (autogamy), 60% choline chloride aqueous solution agent (autogamy).
Implementation of an application example six super quick albumen and active component B and the composite synergy Fructus Cucumidis sativi test of pesticide effectiveness thereof,
This experimental establishment is at Xian District, Shanxi Province suburban area, in Seedling Stage first time dispenser, takes out the second time dispenser of climing phase, the third time dispenser of knot melon phase, dispenser 3 times altogether.Within after each dispenser 10 days, investigate cucumber growth indexes and calculate rate of growth.
Table 13 super quick albumen and active component B and the composite synergy Fructus Cucumidis sativi test of pesticide effectiveness thereof
As can be seen from Table 13, cucumber growth rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, increase yield.
Implementation of an application example seven super quick albumen and active component B and the composite synergy Fructus Lycopersici esculenti test of pesticide effectiveness thereof
This experimental establishment is at Xian District, Shanxi Province suburban area, in nursery stage first time dispenser, setting date second time dispenser, trophophase third time dispenser, dispenser 3 times altogether.Within after each dispenser 10 days, investigate tomato growth indexes and calculate rate of growth.
Table 14 super quick albumen and active component B and the composite synergy Fructus Lycopersici esculenti test of pesticide effectiveness thereof
As can be seen from Table 14, tomato growth rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, increase yield.
Implementation of an application example eight super quick albumen and active component B and the composite synergy Semen Tritici aestivi test of pesticide effectiveness thereof
This experimental establishment is at Xian District, Shanxi Province suburban area, in period of seedling establishment first time dispenser, elongation-heading stage second time dispenser, the third time dispenser of flowering pustulation period, dispenser 3 times altogether.Within after each dispenser 7 days, investigate wheat growth indexes and calculate rate of growth.
Table 15 super quick albumen and active component B and the composite synergy Semen Tritici aestivi test of pesticide effectiveness thereof
As can be seen from Table 15, wheat growth rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, increase yield.
Implementation of an application example nine super quick albumen and active component B and the composite synergy Oryza sativa L. test of pesticide effectiveness thereof
This experimental establishment in Hanzhong City suburb, Shaanxi Province, pustulation period first time dispenser, afterwards every dispenser in 15 days once, altogether dispenser 3 times.Within after each dispenser 7 days, investigate paddy growth indexes and calculate rate of growth.
Table 16 super quick albumen and active component B and the composite synergy Oryza sativa L. test of pesticide effectiveness thereof
As can be seen from Table 16, paddy growth rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, improve yield.
Implementation of an application example ten super quick albumen and active component B and the composite synergy Citrullus vulgaris test of pesticide effectiveness thereof
This experimental establishment is in Weinan City Dali County, Shaanxi Province, in Seedling Stage first time dispenser, stretches the second time dispenser of climing phase phase, third time dispenser before fruiting period, altogether dispenser 3 times.Within after each dispenser 7 days, investigate watermelon growing indexes and calculate rate of growth.
Table 17 super quick albumen and active component B and the composite synergy Citrullus vulgaris test of pesticide effectiveness thereof
As can be seen from Table 17, watermelon growing rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, improve yield.
Implementation of an application example 10 two super quick albumen and active component B and the composite synergy Fructus Vitis viniferae test of pesticide effectiveness thereof
This experimental establishment is in Weinan City Dali County, Shaanxi Province, in young fruit period first time dispenser, colour-change period second time dispenser, dispenser 2 times altogether.Within after each dispenser 10 days, investigate grape growth indexes and calculate rate of growth.
Table 19 super quick albumen and active component B and the composite synergy Fructus Vitis viniferae test of pesticide effectiveness thereof
As can be seen from Table 19, grape growth rate can be significantly improved after super quick albumen is composite with active component B, promote well developed root system, promote the crop effective absorption to fertilizer, improve fruit-setting rate, improve yield.
Invention described above embodiment, is not intended that limiting the scope of the present invention.Any amendment, equivalent replacement and improvement etc. made within the spirit and principles in the present invention, should be included within protection scope of the present invention.
Claims (6)
1. the efficient plant growth containing super quick albumen regulates compositions, effective active composition is active components A and active component B, it is characterized in that: the weight ratio of active components A and active component B is 80 1～1 100, described active components A is super quick albumen, active component B one in CPPU, chlorocholine chloride, choline chloride.
2. the efficient plant growth containing super quick albumen according to claim 1 regulates compositions, it is characterised in that: the weight ratio of active components A and active component B is 60 1～1 80.
3. the efficient plant growth containing super quick albumen according to claim 2 regulates compositions, it is characterised in that:
The weight ratio of super quick albumen and CPPU is 30 1～1 25；
The weight ratio of super quick albumen and chlorocholine chloride is 20 1～1 40；
The weight ratio of super quick albumen and choline chloride is 25 1～1 35.
4. the efficient plant growth containing super quick albumen according to claim 1 regulates compositions, it is characterised in that: wherein effective active component content accounts for the 0.1%～70% of gross weight.
5. the efficient plant growth containing super quick albumen according to claim 4 regulates compositions, it is characterised in that: wherein effective active component content accounts for the 1%～50% of gross weight.
6. the efficient plant growth containing super quick albumen according to claim 1 regulates compositions, it is characterised in that: the dosage form of compositions is selected from wettable powder, water dispersible granules, aqueous emulsion, microemulsion, microcapsule suspending agent, water preparation, soluble liquid or soluble powder.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109699650A (en) * | 2019-01-29 | 2019-05-03 | 中国热带农业科学院环境与植物保护研究所 | A kind of efficient induction lichee generates the composition of disease resistance |
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| CN109699650A (en) * | 2019-01-29 | 2019-05-03 | 中国热带农业科学院环境与植物保护研究所 | A kind of efficient induction lichee generates the composition of disease resistance |
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Address after: 715500 Pucheng high tech Industrial Development Zone, Weinan, Shaanxi Applicant after: Shaanxi Mei Bang Pharmaceutical Group Limited by Share Ltd Address before: 715500 Industrial Park of Agrochemical Base, Pucheng County, Shaanxi Province, Weinan City, Shaanxi Province Applicant before: Shaanxi Meibang Pesticide Co., Ltd. |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160727 |