CN103828828B - Plant growth regulation composition containing plant activator protein - Google Patents
Plant growth regulation composition containing plant activator protein Download PDFInfo
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- CN103828828B CN103828828B CN201210492593.9A CN201210492593A CN103828828B CN 103828828 B CN103828828 B CN 103828828B CN 201210492593 A CN201210492593 A CN 201210492593A CN 103828828 B CN103828828 B CN 103828828B
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- activator protein
- toverticilliumdahliaactivitie activitie
- relative activator
- active component
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- 108091006088 activator proteins Proteins 0.000 title claims abstract description 94
- 239000000203 mixture Substances 0.000 title claims abstract description 43
- 230000008635 plant growth Effects 0.000 title claims abstract description 17
- 239000005962 plant activator Substances 0.000 title abstract description 4
- 230000033228 biological regulation Effects 0.000 title abstract 2
- 230000012010 growth Effects 0.000 claims abstract description 22
- 239000005980 Gibberellic acid Substances 0.000 claims abstract description 21
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims abstract description 21
- IXORZMNAPKEEDV-OBDJNFEBSA-N gibberellin A3 Chemical compound C([C@@]1(O)C(=C)C[C@@]2(C1)[C@H]1C(O)=O)C[C@H]2[C@]2(C=C[C@@H]3O)[C@H]1[C@]3(C)C(=O)O2 IXORZMNAPKEEDV-OBDJNFEBSA-N 0.000 claims abstract description 21
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims abstract description 19
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
- 230000000694 effects Effects 0.000 claims description 104
- 150000005018 aminopurines Chemical class 0.000 claims description 17
- 150000002596 lactones Chemical class 0.000 claims description 17
- 239000000375 suspending agent Substances 0.000 claims description 12
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 10
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 10
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 10
- 240000008067 Cucumis sativus Species 0.000 claims description 10
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 10
- 235000011430 Malus pumila Nutrition 0.000 claims description 10
- 235000015103 Malus silvestris Nutrition 0.000 claims description 10
- 235000021307 Triticum Nutrition 0.000 claims description 9
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 8
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 8
- 240000006365 Vitis vinifera Species 0.000 claims description 8
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000000839 emulsion Substances 0.000 claims description 7
- 239000003094 microcapsule Substances 0.000 claims description 7
- 238000009736 wetting Methods 0.000 claims description 7
- 239000004530 micro-emulsion Substances 0.000 claims description 6
- 239000004562 water dispersible granule Substances 0.000 claims description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 5
- 241000220324 Pyrus Species 0.000 claims description 5
- 240000003768 Solanum lycopersicum Species 0.000 claims description 5
- 235000021017 pears Nutrition 0.000 claims description 5
- 241000675108 Citrus tangerina Species 0.000 claims description 4
- 240000008790 Musa x paradisiaca Species 0.000 claims description 4
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 4
- 238000009472 formulation Methods 0.000 claims description 3
- 241000220225 Malus Species 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 16
- 238000010521 absorption reaction Methods 0.000 abstract description 10
- 239000003337 fertilizer Substances 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 229930002875 chlorophyll Natural products 0.000 abstract description 2
- 235000019804 chlorophyll Nutrition 0.000 abstract description 2
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 239000004480 active ingredient Substances 0.000 abstract 4
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 abstract 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 abstract 1
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 239000003617 indole-3-acetic acid Substances 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 230000021749 root development Effects 0.000 abstract 1
- 230000009758 senescence Effects 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 29
- 239000002131 composite material Substances 0.000 description 21
- 239000003814 drug Substances 0.000 description 15
- 239000000575 pesticide Substances 0.000 description 13
- 230000002195 synergetic effect Effects 0.000 description 12
- 239000008367 deionised water Substances 0.000 description 11
- 229910021641 deionized water Inorganic materials 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 9
- 244000141359 Malus pumila Species 0.000 description 9
- 230000002528 anti-freeze Effects 0.000 description 9
- 239000002270 dispersing agent Substances 0.000 description 9
- 239000000080 wetting agent Substances 0.000 description 9
- 241000209140 Triticum Species 0.000 description 8
- 239000013530 defoamer Substances 0.000 description 8
- 239000000470 constituent Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- 239000003995 emulsifying agent Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 238000011835 investigation Methods 0.000 description 6
- 239000002562 thickening agent Substances 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000012071 phase Substances 0.000 description 4
- 239000005648 plant growth regulator Substances 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 230000002786 root growth Effects 0.000 description 4
- 206010011732 Cyst Diseases 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 208000031513 cyst Diseases 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 240000001987 Pyrus communis Species 0.000 description 2
- 235000014443 Pyrus communis Nutrition 0.000 description 2
- 230000010165 autogamy Effects 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 239000007884 disintegrant Substances 0.000 description 2
- 239000002552 dosage form Substances 0.000 description 2
- RSQSQJNRHICNNH-NFMPGMCNSA-N gibberellin A4 Chemical compound C([C@@H]1C[C@]2(CC1=C)[C@H]1C(O)=O)C[C@H]2[C@@]2(OC3=O)[C@H]1[C@@]3(C)[C@@H](O)CC2 RSQSQJNRHICNNH-NFMPGMCNSA-N 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000003002 pH adjusting agent Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000223602 Alternaria alternata Species 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- -1 aqua Substances 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 238000000498 ball milling Methods 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000006184 cosolvent Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000010534 mechanism of action Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000002787 reinforcement Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a plant growth regulation composition containing plant activator protein. The composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from the plant activator protein; the active ingredient B is selected from any one of compounds as follows: benzyl aminopurine, brassinolide, indoleacetic acid, harpin protein and gibberellic acid. The composition is mainly used for increasing the plant chlorophyll content, keeping plants green, preventing plant senescence, promoting root development and effective absorption of crops on fertilizers, assisting in good growth of weak parts of the crops, enhancing the absorption of the plants on water and the fertilizers and regulating the balance of the water in the plants so as to improve the drought resistance and the cold resistance of the plants.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of application of plant growth regualting composition crops containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein.
Technical background
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein (plant activator protein) molecular formula: C
963h
1564o
342n
280s
3, chemical name: 207 peptides proteins.ToVerticilliumdahliaActivitie Activitie S of Relative activator protein makes plant growth accelerate vigor enhancing, the increase of proline content and the reinforcement of cellulase by the metabolic system of activated plant, promote that plant cell increases, improving activity of root system is strengthened, and reaches volume increase object.
Benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid are conventional plant growth regulator, and wherein gibberellic acid is gibberellic acid A4+A7, gibberellic acid A3, gibberellic acid A4, gibberellic acid A7.
For target plant, plant growth regulator is the non-nutritive chemical substance of external source, usually can conduct to site of action in plant corpus, just can promote or suppress some link of its life process with very low concentration, makes it the needs development to meeting the mankind.Every plant growth regulators has specific purposes, and application technology requires quite strict, only could produce specific effect to target plant under specific application conditions (comprising extraneous factor).Often change concentration and will obtain contrary result, such as, have facilitation at low concentrations, then become inhibitory action in higher concentrations.Active components A is different from the active component B mechanism of action, mutually composite have good synergistic effect within the specific limits, and about the binary built of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid, there is not yet relevant report at home and abroad at present.
Summary of the invention
The object of the invention is to propose a kind of to there is low, the effective plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein of synergistic function, use cost.
The present invention is achieved through the following technical solutions:
A kind of plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein, composition is active components A and active component B, the weight ratio of active components A and active component B is 5000:1 ~ 1:200, described active components A is selected from toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B is selected from the one in benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid, and in composition, the more excellent weight ratio of active components A and active component B is 3000:1 ~ 1:100; Wherein the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine is 200:1 ~ 1:50, the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones is 2000:1 ~ 500:1, the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin is 2500:1 ~ 800:1, the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen is 200:1 ~ 1:50, and the weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid is 200:1 ~ 1:50.
Described plant growth regulator composition, wherein effective active component content accounts for 0.0001% ~ 60% of gross weight, is preferably 0.001% ~ 20%.
The described plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein is for promoting the application of pears, apple, grape, oranges and tangerines, banana, Chinese cabbage, cucumber, tomato, wheat or paddy growth.
Surfactant selected by plant growth regualting composition of the present invention is known in those skilled in the art: can be selected from one or more in dispersant, wetting agent, thickener or defoamer.According to different dosage form, in preparation, stabilizing agent known in those skilled in the art, antifreeze etc. can also be contained.
Plant growth regualting composition of the present invention, can be processed into acceptable formulation on any agricultural chemicals on demand.Wherein preferred dosage form is wetting powder, water dispersible granules, suspending agent, aqueous emulsion, microemulsion, aqua, microcapsule suspending agent, can also make soluble powder, soluble liquid, effervescent agent, suspension emulsion, microcapsule suspension-suspendinagent agent.
Composition comprises following constituent content when making wetting powder: active components A 0.1% ~ 50%, active component B0.0001% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
Composition comprises following constituent content when making water dispersible granules: active components A 0.1% ~ 50%, active component B0.0001% ~ 50%, dispersant 3% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
Composition comprises following constituent content when making suspending agent: active components A 0.1% ~ 40%, active component B0.0001% ~ 40%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, defoamer 0.01% ~ 2%, thickener 0 ~ 2%, antifreeze 0 ~ 8%, deionized water add to 100%.
Composition comprises following constituent content when making aqueous emulsion: active components A 0.1% ~ 40%, active component B 0.0001% ~ 40%, solvent 1% ~ 30%, emulsifier 1% ~ 15%, antifreeze 0 ~ 8%, thickener 0 ~ 2%, defoamer 0.01% ~ 2%, deionized water add to 100%.
Composition comprises following constituent content when making microemulsion: active components A 0.1% ~ 40%, active component B0.0001% ~ 40%, solvent 1% ~ 20%, emulsifier 3% ~ 25%, antifreeze 0 ~ 8%, defoamer 0.01% ~ 2%, deionized water add to 100%.
Composition comprises following constituent content when making aqua: active components A 0.1% ~ 40%, active component B0.0001% ~ 40%, cosolvent 2% ~ 6%, wetting agent 1%-10%, antifreeze 0 ~ 8%, deionized water add to 100%.
Composition comprises following constituent content when making microcapsule suspending agent: active components A 0.1% ~ 40%, active component B0.0001% ~ 40%, macromolecule cyst material 2% ~ 10%, dispersant 1% ~ 10%, solvent 1% ~ 10%, emulsifier 1% ~ 7%, defoamer 0.01% ~ 2%, pH adjusting agent 0.01% ~ 5%, deionized water add to 100%.
Wetting powder the key technical indexes of the present invention:
Water dispersible granules the key technical indexes of the present invention:
Suspending agent the key technical indexes of the present invention:
Aqueous emulsion the key technical indexes of the present invention:
Microemulsion the key technical indexes of the present invention:
Aqua the key technical indexes of the present invention:
Microcapsule suspending agent the key technical indexes of the present invention:
The invention has the advantages that:
(1) toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid are composite has obvious growth promoting effects effect afterwards to pears, apple, grape, oranges and tangerines, banana, Chinese cabbage, cucumber, tomato, wheat or paddy rice; (2) increase chlorophyll content of plant, protect green anti-old, promote well developed root system, promote that crop is to effective absorption of fertilizer; (3) supplemental crop inferior position part well grows, and strengthens plant to water, fertile absorption, regulates the balance of moisture in plant body, thus improve drought resisting, the cold resistance of plant.
Embodiment
Below in conjunction with embodiment to further instruction of the present invention, the percentage in embodiment is all weight percentage, but the present invention is not limited thereto.
Application Example one
Embodiment 1 ~ 15 wetting powder
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, dispersant, wetting agent, filler are mixed in mixed cylinder, mixes again after airslide disintegrating mill is pulverized, wetting powder product of the present invention can be obtained, specifically in table 1.
The each component of table 1 embodiment 1 ~ 15 and weight portion
Embodiment 16 ~ 30 water dispersible granules
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained together the particle diameter needed through air-flow crushing, then add other auxiliary agents such as binding agent (can add and can not add), obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through granulating and after drying, water dispersible granules product of the present invention can being obtained, specifically in table 2.
The each component of table 2 embodiment 16 ~ 30 and weight portion
Embodiment 31 ~ 40 suspending agent
By dispersant, wetting agent, defoamer, thickener (can add and can not add), antifreeze (can add and can not add), mix through high speed shear, add toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, surplus deionized water is supplied, and can obtain suspending agent product of the present invention, specifically in table 3.
The each component of table 3 embodiment 31 ~ 40 and weight portion
Embodiment 41 ~ 50 aqueous emulsion
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, solvent, emulsifier are added together, make to be dissolved into homogeneous oil phase; Deionized water, antifreeze (can add and can not add), thickener (can add and can not add), defoamer are mixed, becomes homogeneous aqueous phase.Under high velocity agitation, aqueous phase is added oil phase, surplus deionized water is supplied; Aqueous emulsion product of the present invention can be obtained, specifically in table 4.
The each component of table 4 embodiment 41 ~ 50 and weight portion
Embodiment 51 ~ 55 microemulsion
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B are dissolved in and are equipped with in the homogenizer of solvent, emulsifier, antifreeze (can add and can not add), defoamer are joined in the homogenizer that above-mentioned solution is housed, surplus deionized water gives strong mixing and homogenize after supplying, finally obtain the microemulsion product of the present invention that clear appearance is transparent.Specifically in table 5.
The each component of table 5 embodiment 51 ~ 55 and content
Embodiment 56 ~ 73 aqua
ToVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, wetting agent, antifreeze (can add and can not add), solvent, deionized water etc. are mixed together, the aqua product of the obtained present composition, specifically in table 6.
The each component of table 6 embodiment 56 ~ 73 and weight portion
Embodiment 74 ~ 78 microcapsule suspending agent
By toVerticilliumdahliaActivitie Activitie S of Relative activator protein, active component B, macromolecule cyst material, solvent, make to be dissolved into homogeneous oil phase, in a shear condition, oil phase is joined in the aqueous phase solution containing emulsifier, pH adjusting agent, dispersant, surplus deionized water is supplied, bi-material reacts at oil-water interfaces, forms macromolecule cyst wall, makes the finely disseminated microcapsule suspending agent product of the present composition.Specifically in table 7.
The each component of table 7 embodiment 74 ~ 78 and weight portion
Benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid in table 1 ~ 7 are exchanged, can novel formulation be obtained.
This measuring adopts growth of seedling method.Determination step is as follows:
Selecting seed of the same size dials in the culture dish of the 9cm size being covered with two layers of filter paper respectively, and 10, every ware adds the liquid 1.5ml configured, and contrast adding distil water, repeats 4 times.Cover ware lid, the artificial climate being placed into 28 ± 1 DEG C is indoor, illumination 10 hours.In 6 days check heights of seedling and root long.
Result statistical analysis:
Application Example two:
For studying thing: Chinese cabbage
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: through the promotion concentration range of preliminary experiment determination toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the former medicine of benayl aminopurine and the two different ratio mixture.
Result of the test:
The mixed liquid of table 8 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine and different proportion thereof is to the growth-promoting effect of Chinese cabbage
As can be seen from Table 8 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine used in combination after, seedling and root growth can be promoted simultaneously, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine proportioning in the scope of 5000:1 ~ 1:200, all there is stronger synergistic effect, especially when the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine is at 200:1 ~ 1:50, synergistic effect is more obviously given prominence to, testing through applicant the optimum ratio finding toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine is 200:1, 100:1, 50:1, 25:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:12, 1:15, 1:18, 1:20, 1:50, be wherein that 1 ︰ 2 effect is best with toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine.
Application Example three:
For studying thing: cucumber
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: through the promotion concentration range of preliminary experiment determination toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the former medicine of brassin lactones and the two different ratio mixture.
Result of the test:
The mixed liquid of table 9 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones and different proportion thereof is to the growth-promoting effect of cucumber
As can be seen from Table 9 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones used in combination after, seedling and root growth can be promoted simultaneously, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones proportioning in the scope of 5000:1 ~ 1:200, all there is stronger synergistic effect, especially when the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones is at 2000:1 ~ 500:1, synergistic effect is more obviously given prominence to, test through applicant, the optimum ratio of discovery toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones is 2000:1, 1500:1, 1300:1, 1200:1, 1100:1, 1050:1, 1025:1, 1015:1, 1010:1, 1008:1, 1006:1, 1004:1, 1003:1, 1002:1, 1001:1, 1000:1, 999:1, 998:1, 997:1, 996:1, 995:1, 980:1, 800:1, 600:1, 500:1, wherein with toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones for 1000:1 effect is best.
Application Example four:
For studying thing: apple tree
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: through the promotion concentration range of preliminary experiment determination toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the former medicine of heteroauxin and the two different ratio mixture.
Result of the test:
The mixed liquid of table 10 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin and different proportion thereof is to the growth-promoting effect of apple tree
As can be seen from Table 10 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin used in combination after, fruit bearing rate and fruit growth can be promoted simultaneously, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin proportioning in the scope of 5000:1 ~ 1:200, all there is stronger synergistic effect, especially when the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin is at 2500:1 ~ 800:1, synergistic effect is more obviously given prominence to, test through applicant, the optimum ratio of discovery toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin is 2500:1, 2000:1, 1800:1, 1650:1, 1625:1, 1615:1, 1610:1, 1605:1, 1603:1, 1601:1, 1600:1, 1599:1, 1595:1, 1590:1, 1580:1, 1550:1, 1500:1, 1000:1, 800:1, wherein with toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin for 1600:1 effect is best.
Application Example five:
For studying thing: wheat
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: through the promotion concentration range of preliminary experiment determination toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick proteinogen medicine and the two different ratio mixture.
Result of the test:
The mixed liquid of table 11 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen and different proportion thereof is to the growth-promoting effect of wheat
As can be seen from Table 11 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen used in combination after, seedling and root growth can be promoted simultaneously, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen proportioning in the scope of 5000:1 ~ 1:200, all there is stronger synergistic effect, especially when the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen is at 200:1 ~ 1:50, synergistic effect is more obviously given prominence to, test through applicant, the optimum ratio of discovery toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen is 200:1, 100:1, 50:1, 25:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:12, 1:15, 1:18, 1:20, 1:50, be wherein that 1 ︰ 2 effect is best with toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen.
Application Example six:
For studying thing: paddy rice
Test medicine provides by Shanxi Thompson Biological Technology Co., Ltd..
Experimental scheme: through the promotion concentration range of preliminary experiment determination toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the former medicine of gibberellic acid and the two different ratio mixture.
Result of the test:
The mixed liquid of table 12 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid and different proportion thereof is to the growth-promoting effect of paddy rice
As can be seen from Table 12 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid used in combination after, seedling and root growth can be promoted simultaneously, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid proportioning in the scope of 5000:1 ~ 1:200, all there is stronger synergistic effect, especially when the proportioning of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid is at 200:1 ~ 1:50, synergistic effect is more obviously given prominence to, test through applicant, the optimum ratio of discovery toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid is 200:1, 100:1, 50:1, 25:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:12, 1:15, 1:18, 1:20, 1:50, be wherein that 1 ︰ 2 effect is best with toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid.
Effect experiment part: test medicine is researched and developed by Shanxi Thompson Biological Technology Co., Ltd., provided, contrast medicament 3% toVerticilliumdahliaActivitie Activitie S of Relative activator protein wetting powder (autogamy), the solvable pulvis of 1% benayl aminopurine (commercial), 0.004% brassin lactones aqua (commercial), 0.11% heteroauxin aqua (commercial), 3% super quick protein particle agent (autogamy), 40% gibberellic acid soluble granule (commercial).
Implementation of an application example seven toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with Chinese cabbage thereof
This experimental establishment is in Weinan City suburb, Shaanxi Province, and in thanking to florescence first time dispenser, the 2nd dispenser in 15 days after medicine, is total to dispenser 2 times for the first time.7 days investigation Chinese cabbage growing indexs after each dispenser also calculate growth rate.
Table 13 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with Chinese cabbage thereof
As can be seen from Table 13, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve Chinese cabbage growth rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
Implementation of an application example ends of the earth alternaria tenuis activator protein and active component B and the composite test of pesticide effectiveness cooperating with cucumber thereof
This experimental establishment is at Shaanxi Province's Weinan City, and in thanking to florescence first time dispenser, the 2nd dispenser in 15 days after medicine, is total to dispenser 2 times for the first time.7 days investigation cucumber growth indexes after each dispenser also calculate growth rate.
Table 14 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with cucumber thereof
As can be seen from Table 14, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve cucumber growth rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
Implementation of an application example nine toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with wheat thereof
This experimental establishment is in Weinan City Pucheng County, Shaanxi Province, and in thanking to florescence first time dispenser, the 2nd dispenser in 15 days after medicine, is total to dispenser 2 times for the first time.7 days investigation tomato growth indexes after each dispenser also calculate growth rate.
Table 15 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with wheat thereof
As can be seen from Table 15, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve wheat growth rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
Implementation of an application example ten toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with apple tree thereof
This experimental establishment is at Shaanxi Province's Weinan City Baishui County, and in thanking to florescence first time dispenser, second time dispenser after bagging, plucks the front third time dispenser of bag, dispenser 3 times altogether.10 days investigation apple development indexes after each dispenser also calculate growth rate.
Table 16 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with apple tree thereof
As can be seen from Table 16, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve apple development rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
Implementation of an application example 11 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with pear tree thereof
This experimental establishment is at Xianyang, Shanxi province suburban area, and in thanking to florescence first time dispenser, second time dispenser after bagging, plucks the front third time dispenser of bag, dispenser 3 times altogether.10 days investigation Growth of Pear Trees indexes after each dispenser also calculate growth rate.
Table 17 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with pear tree thereof
As can be seen from Table 17, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve pears growth rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
Implementation of an application example 12 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with grape thereof
This experimental establishment is in Yangling District, Xianyang, Shanxi province city, and in thanking to florescence first time dispenser, the dispenser of fruit expanding period second time, third time dispenser before the maturing stage, is total to dispenser 3 times.7 days investigation grape growth indexes after each dispenser also calculate growth rate.
Table 18 toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B and the composite test of pesticide effectiveness cooperating with grape thereof
As can be seen from Table 18, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and active component B composite after can significantly improve grape growth rate, promote well developed root system, promote crop to effective absorption of fertilizer, improve fruit-setting rate.
After draw through tests different local throughout the country, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid are composite all has obvious growth promoting effects effect afterwards to pears, apple, grape, oranges and tangerines, banana, Chinese cabbage, cucumber, tomato, wheat or paddy rice.
Above-described embodiment of the present invention, does not form limiting the scope of the present invention, and any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein, composition effective active composition is active components A and active component B, it is characterized in that: the weight ratio of active components A and active component B is 5000:1 ~ 1:200, described active components A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is selected from the one in benayl aminopurine, brassin lactones, heteroauxin, super quick albumen, gibberellic acid.
2. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 1, is characterized in that: in composition, the weight ratio of active components A and active component B is 3000:1 ~ 1:100.
3. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 2, is characterized in that:
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and benayl aminopurine is 200:1 ~ 1:50;
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and brassin lactones is 2000:1 ~ 500:1;
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and heteroauxin is 2500:1 ~ 800:1;
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and super quick albumen is 200:1 ~ 1:50;
The weight ratio of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and gibberellic acid is 200:1 ~ 1:50.
4. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 1, is characterized in that: wherein effective active component content accounts for 0.0001% ~ 60% of gross weight.
5. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 4, is characterized in that: wherein effective active component content accounts for 0.001% ~ 20% of gross weight.
6. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 1, is characterized in that: the formulation of composition is selected from wetting powder, water dispersible granules, suspending agent, aqueous emulsion, microemulsion, aqua or microcapsule suspending agent.
7. the plant growth regualting composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein according to claim 1 is for promoting the application of pears, apple, grape, oranges and tangerines, banana, Chinese cabbage, cucumber, tomato, wheat or paddy growth.
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CN104604946B (en) * | 2015-01-12 | 2017-10-13 | 邱德文 | It is a kind of for biologic protein agents of prevention and control Citrus Huanglongbing pathogen and preparation method thereof |
CN104911108B (en) * | 2015-03-23 | 2018-02-27 | 辽宁省农业科学院 | The preparation method and purposes of sea-buckthorn endogenetic fungus and its extract |
UY37179A (en) * | 2016-04-04 | 2017-10-31 | Univ Pública De Navarra | COMPOSITIONS PRODUCED BY MICROORGANISMS WITH STIMULATING ACTIVITY ON PLANTS |
CN106259464A (en) * | 2016-04-29 | 2017-01-04 | 江苏辉丰农化股份有限公司 | Containing Thidiazuron and the plant growth regulator composition of super quick albumen |
CN106561720B (en) * | 2016-10-11 | 2019-04-16 | 江苏辉丰农化股份有限公司 | The bactericidal composition of class containing benzoisothiazolinone and toVerticilliumdahliaActivitie Activitie S of Relative activator protein |
CN106962406B (en) * | 2016-11-10 | 2019-10-25 | 江苏辉丰生物技术有限公司 | Composition comprising Thidiazuron and toVerticilliumdahliaActivitie Activitie S of Relative activator protein |
CN108308177B (en) * | 2018-02-06 | 2020-08-04 | 北京航空航天大学 | Activator protein PeaT1 nano particle and application thereof in crop disease resistance and growth promotion |
CN109169740A (en) * | 2018-06-21 | 2019-01-11 | 惠州市东江园林工程有限公司 | A kind of biological pesticide being conducive to environmental resource |
CN110205249B (en) * | 2019-06-12 | 2021-02-26 | 中国矿业大学(北京) | Method for promoting plant growth and alternaria alternate fungus used by same |
CN113875778A (en) * | 2021-09-29 | 2022-01-04 | 季红进 | Biostimulant comprising plant immunoactive protein and 5-aminolevulinic acid or derivative thereof |
CN117204427B (en) * | 2023-11-08 | 2024-02-23 | 西北农林科技大学深圳研究院 | Agricultural composition for apple flower thinning and fruit thinning |
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