CN105777262A - Growth-promoting bioorganic fertilizer special for tobacco and application thereof - Google Patents
Growth-promoting bioorganic fertilizer special for tobacco and application thereof Download PDFInfo
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- CN105777262A CN105777262A CN201610158302.0A CN201610158302A CN105777262A CN 105777262 A CN105777262 A CN 105777262A CN 201610158302 A CN201610158302 A CN 201610158302A CN 105777262 A CN105777262 A CN 105777262A
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- fertilizer
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- nicotiana tabacum
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a growth-promoting bioorganic fertilizer special for tobacco and application thereof. A preparation method of this fertilizer comprises: inoculating Bacillus subtilis, Bacillus licheniformis and Streptomyces rochei respectively to a beef extract peptone liquid medium, and vibrating and centrifuging to collect bacteria; inoculating the three bacteria to a sterilized organic fertilizer carrier; adjusting water content of the organic fertilizer, and fermenting to obtain the growth-promoting bioorganic fertilizer special for tobacco. The growth-promoting bioorganic fertilizer special for tobacco can significantly increase flue-cured tobacco yield and quality, can effectively improve microbial abundance of soil, is good for nutritional conversion of soil microbes to promote the growth of tobacco plants, can also reduce the usage of pesticides and chemical fertilizers and has good popularization value.
Description
Technical field
The present invention relates to one to grow tobacco special growth-promoting biological organic fertilizer and application thereof, belong to Nicotiana tabacum L. growth-promoting organic fertilizer technical field.
Background technology
Plant growth-promoting rhizobacteria (plantgrowthpromotingrhizobacteria, PGPR) refer to that free living can promote plant growing and to the absorption of mineral nutrition and utilization at soil or the class in root system of plant of growing nonparasitically upon another plant, and the useful mushroom of harmful organism can be suppressed..Big quantity research confirms that PGPR is widely present in the rhizosphere of various plants, has identified multiple PGPR bacterial strain wherein main species at present and has included bacillus (Bacillus) and Rhodopseudomonas (Pseudomonas).In recent years, along with the high speed development of industry, environmental pollution is further exacerbated by, and the environmental consciousness of people is strengthened day by day, thirsts for finding a kind of consumption that makes that can not only reduce pesticide and chemical fertilizer, can not also affect the effective means even promoting plant growing simultaneously.Under such realistic background, the research of plant rhizosphere growth-promoting antibacterial is increasingly received publicity.Research and application about PGPR at present is concentrated mainly on grain and cotton crop as important in Oryza sativa L., Wheat and Corn and Cotton etc., and about the research of tobacco rhizosphere Promoting bacteria and application, current domestic report focuses mainly on the Biocontrol Effect aspect of Nicotiana tabacum L., and the aspect researchs such as tobacco in field growth-promoting is very few.
Summary of the invention
The technical problem to be solved in the present invention is: provide one to grow tobacco special growth-promoting biological organic fertilizer and application thereof, yield of flue-cured tobacco and quality are had the effect of being significantly improved by it, and can effectively improve soil bacterial abundance, the nutrition being beneficial to soil microorganism converts and then promotes the growth of cigarette strain, that can also reduce pesticide and chemical fertilizer makes consumption simultaneously, it is possible to overcome the deficiencies in the prior art.
Technical scheme: one grows tobacco special growth-promoting biological organic fertilizer, and its preparation method is: respectively bacillus subtilis, Bacillus licheniformis and Lou Che Shi streptomycete are inoculated in beef extract-peptone fluid medium, vibration, collected after centrifugation thalline；Three kinds of thalline are inoculated in the fertilizer carrier after sterilizing；Regulate fertilizer water content and ferment, obtaining the special growth-promoting biological organic fertilizer of Nicotiana tabacum L..
The amino acid fertilizer that described fertilizer carrier is rapeseed cake enzymolysis mixes with mass ratio 1:1 with cattle manure, containing organic matter 33.8%, aminoacid 4.3%, N4.20%, P2O52.26%, K2O1.08%.
Described vibration, centrifugal condition be: 30 DEG C, vibrate 48h, fermentation liquid 6000 × g of 170rpm be centrifuged 10min.
Regulating fertilizer water content is 40%.
Fermentation time is 5-6 days.
Thalline quantity after fermentation is more than 108cfug-1.
The application after tobacco in seedling stage and transplanting of the described Nicotiana tabacum L. special growth-promoting biological organic fertilizer.
Beneficial effects of the present invention:
Compared with prior art, after using the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. of the present invention, it is possible to obtain good quality and high output flue-cured tobacco, simultaneously root dry weight, root diameter, root area and root volume also have and improve largely；Resistance enzyme in blade is lived and is also significantly improved.By inventor's growth-promoting biological organic fertilizer field experiment, biological organic fertilizer of the present invention processes has obvious growth-promoting functions to Nicotiana tabacum L., has good promotional value.
Accompanying drawing explanation
Fig. 1 different disposal soil microorganism abundance dendrogram；In figure: PGCK, comparison；PGO, processes two；PGS: process five；PGM: process six.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearly, below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment:
1, prepared by growth-promoting biological organic fertilizer
Respectively Promoting bacteria bacillus subtilis, Bacillus licheniformis and Lou Che Shi streptomycete are inoculated in beef extract-peptone fluid medium, 30 DEG C, 170rpm vibrates 48h.Fermentation liquid 6000 × g is centrifuged 10min, collects thalline.By 5% inoculum concentration, thalline is inoculated in the fertilizer carrier after sterilizing.Regulating fertilizer water content is 40%, ferments 5-6 days.After fermentation ends, determine that Promoting bacteria quantity reaches 10 by dilution plate gradient counting8cfug-1, thus making No. 1 growth-promoting bacterial manure, No. 2 growth-promoting bacterial manure and No. 3 growth-promoting bacterial manure, compound growth-promoting bacterial manure is 1,2 and No. 3 compound Mix growth-promoting bacterial manure.The amino acid fertilizer that fertilizer carrier is rapeseed cake enzymolysis mixes with 1:1 (mass ratio) with cattle manure, containing organic matter 33.8%, aminoacid 4.3%, N4.20%, P2O52.26%, K2O1.08%.
2, EXPERIMENTAL DESIGN
Field experiment sets 6 process altogether: conventional fertilizer application comparison (T1), conventional fertilizer application+fertilizer (T2) ,+No. 1 growth-promoting bacterial manure (T3) conventional fertilizer application of conventional fertilizer application+No. 3 growth-promoting bacterial manure (T5) conventional fertilizer applications of+No. 2 growth-promoting bacterial manure (T4) conventional fertilizer applications+compound growth-promoting bacterial manure (1+2+3) (T6).Conventional fertilizer application is N consumption 75kg/hm2, pure phosphorus 45kg/hm2, potassium 150kg/hm2, fertilizer 600kg/hm2.Nitrogenous fertilizer carbamide (N46%), phosphate fertilizer calcium superphosphate (P2O512%), potash fertilizer potassium chloride (K2O60%).The amino acid fertilizer that fertilizer is rapeseed cake enzymolysis mixes with 1:1 (mass ratio) with cattle manure, containing organic matter 33.8%, aminoacid 4.3%, N4.20%, P2O52.26%, K2O1.08%, base manure 45kg/hm2, topdress 30kg/hm2；Base manure N:P:K=10:10:25, topdress N:P:K=13:0:26.Adopt randomized complete-block design, often process 3 times and repeat.Nicotiana tabacum L. was transplanted April 27, and JIUYUE results on the 10th, Transplanting Density is 1m × 0.6m, every community 90 strain.
3, test site, soil and weather
Field test carries out (106 ° of 07'57.6 " N, 26 ° of 11'34.5 " E) at dragon hilllock, Guizhou Province Tabacco Science and Technology Institute experimental station, average temperature of the whole year 15 DEG C, annual rainfall 1197mm, annual frost-free period average 276.5d.For examination soil pH 5.45, organic 48.6g/kg, full nitrogen 2.29g/kg, alkali-hydrolyzable nitrogen 158mg/kg, rapid available phosphorus 48.9mg/kg, available potassium 543mg/kg.
4, sampling and assay method
4.1 tobacco productions and the output value measure
After tobacco transplant about 60-70 days, blade started ripe gradually from bottom to top, and according to Nicotiana tabacum L. mature condition Several harvests from the bottom to top and be put in barn and toast, cured tobacco leaf presentation quality and Yield and quality analysis are according to national standard " flue-cured tobacco GB2635-1992 ".Each community Nicotiana tabacum L. is carried out grade determination, and measures the yield of each grade Nicotiana tabacum L. and better-than-average cigarette ratio respectively, calculate the output value and average price by tobacco purchasing valency then.
4.2 flue-cured tobacco agronomic shape investigation and root system measure
With reference to countries tobacco industry standard Nicotiana tabacum L. economical character investigation method.
The mensuration analysis that 4.3 enzymes are lived
Group's phase, prosperous long-term and period of maturation acquisition test district Nicotiana tabacum L., cigarette root and pedotheque respectively after transplanting；Guaiacol algoscopy is adopted to measure peroxidase (POD) for Nicotiana tabacum L. and cigarette strain root soil sample；Hydrogen peroxide determined by ultraviolet spectrophotometry catalase (CAT)；Nitroblue tetrazolium (NBT) method measures superoxide dismutase (SOD)；Catechol method measures polyphenol oxidase (PPO), measures improving activity of root system and Root morphology simultaneously.
5, result and analysis
The index such as tobacco production and the output value under 5.1 field test different disposal
Without significant difference in the per mu yield that T6 and T4 processes, but it is significantly higher than other and processes, process volume increase 5.95%, 8.91% and 12.35% than T5, T3 and T2 respectively.T1 processes and is substantially less than other process in Nicotiana tabacum L. per mu yield value, processes minimizing 4.34%, 3.51%, 11.46%, 10.98% and 13.74% than T2, T3, T4, T5 and T6 respectively, and the per mu yield value of other process is without significant difference.Without significant difference (table 1) between each process in good smoke rate, better-than-average cigarette rate and average price.
The index such as yield and the output value of Nicotiana tabacum L. under table 1 different disposal
Note: in table, numerical value is the average repeated 3 times；Significant difference is there is in P≤0.05 level between the different letter representation every process of same soil.Lower same
The economical character of 5.2 different fertilization different times Nicotiana tabacum L.s
In group's phase, respectively processing no significant difference on economical character, but T6 process is all maximum in all indexs, this also lays a solid foundation for well growing of later stage Nicotiana tabacum L. below.After entering Topping Stage, without significant difference on the plant height of T6 and T4, but being significantly higher than other and process, process raising 7.26%, 5.59% and 4.05% than T5, T3 and T2 respectively, T1 processes and is substantially less than other process on Nicotiana tabacum L. plant height.The T4 number of blade is significantly higher than T2, T3 and T5 and processes, and without significant difference, zero difference between T2, T3, T5 and T1 process between T6 process.T6 process maximum width of blade and T4 process between without significant difference, but be significantly higher than other process, T2, T3 and T5 process between zero difference but be significantly higher than T1 process.The maximum leaf length that T6 processes be also significantly greater than other process but and T4 process between no significant difference, other respectively process between without significant difference.T6 process stem girth and T4 and T3 process between without significant difference but be significantly higher than other process (table 2).
The economical character of Nicotiana tabacum L. under table 2 different fertilization
The change of the root system of 5.3 different fertilization Nicotiana tabacum L.s
Without significant difference on the root dry weight that T6 and T4 processes, but it is significantly higher than other and processes, process increase by 10.44%, 8.47% and 8.04% (table 3) than T5, T3 and T2 respectively.T1 processes and is substantially less than other process on root dry weight, processes minimizing 8.41%, 8.04%, 13.88%, 6.42% and 15.22% than T2, T3, T4, T5 and T6 respectively, and the root dry weight of other process is without significant difference.At root diametrically, it is upper without significant difference that T6 and T4 processes, but be significantly higher than other and process, no significant difference between other process.Without significant difference on the root area that T6 and T3, T4 process, but being significantly higher than other and process, T1 root area is minimum.The root volume of T6 is maximum, is significantly higher than other and processes, and without significant difference between T4 and T3 process, but is significantly higher than T5 and T2 and processes, and the root system that same conventional fertilizer application processes is minimum, is only 0.48m3/plant.
The change of table 3 different fertilization tobacco root
The enzyme of 5.4 different fertilization different times Nicotiana tabacum L.s is lived and is changed
In group's phase, most of enzyme that T6 processes index (except peroxidase) alive is maximum, but and without significant difference between T4 process, it is significantly higher than other simultaneously and processes；T5 processes peroxidase and is substantially less than T6 and T4 process but is significantly higher than T3 and T2 process；The catalase that T2 processes is substantially less than T6 process but is significantly higher than T5 and T3 and processes；T5, T3 and T2 process between superoxide dismutase without significant difference, but are significantly higher than T1 process, and polyphenol oxidase there is also identical trend.Enter prosperous long-term after, peroxidase, catalase and the superoxide dismutase that T6 processes is all remarkably higher than other and processes, and the polyphenol oxidase that T4 processes is significantly higher than other and processes, four enzymes that T5, T3 and T2 process are lived between index without significant difference, but being above T1 process, all enzymes that T1 processes index alive is minimum.
Table 4 different disposal different times tobacco leaf enzyme is lived and is changed
The impact on soil microorganism abundance level of 5.5 different fertilization
Based on the abundance level of species in each sample, it is achieved species taxonomy status cluster (laterally cluster) and sample clustering (longitudinally cluster), find out and sample is assembled more species.Different colours means the relative abundance level (Fig. 1) of species in sample.As can be seen from Figure 1 the different species abundances in fertilizer treatment soil is entirely different, the Nitrospira wherein processing six (PGM) is a class gram negative bacteria, belong to nitrated spiral Pseudomonas (Nitrospira), nitrite-oxidizing can be become nitrate as nitrobacteria (Nitrifier), it is that soil microorganism carries out nitrogen transformation Major Function strain, and then can tentatively disclose the reason that process process six (PGM) cigarette strain production is vigorous.
In summary, the growth-promoting biological organic fertilizer being made up of three kinds of Promoting bacteria all has significant growth-promoting effect to after tobacco in seedling stage and transplanting, compound growth-promoting fertilizer processes and is significantly higher than other process, volume increase 14.21% and 12.35% is processed respectively than conventional fertilizer application, conventional fertilizer application+fertilizer, the Agronomic character that compound growth-promoting fertilizer processes is maximum in group and Topping Stage, but and without significant difference between No. 1 growth-promoting fertilizer process；Root dry weight, root diameter, root area and root volume ratio conventional fertilizer application that compound organic fertilizer processes process and increase by 17.96%, 12.64%, 82.35% and 1.68 times respectively；The compound growth-promoting fertilizer process alive of resistance enzyme in blade is also significantly greater than other and processes, and rhizosphere microorganism species abundance performance difference, wherein the Nitrospira abundance of compound growth-promoting fertilizer process is significantly higher than conventional fertilizer application process.
Claims (7)
1. one grow tobacco special growth-promoting biological organic fertilizer, it is characterised in that: its preparation method is: bacillus subtilis, Bacillus licheniformis and Lou Che Shi streptomycete are inoculated in beef extract-peptone fluid medium, vibration, collected after centrifugation thalline respectively；Three kinds of thalline are inoculated in the fertilizer carrier after sterilizing；Regulate fertilizer water content and ferment, obtaining the special growth-promoting biological organic fertilizer of Nicotiana tabacum L..
2. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. according to claim 1, it is characterised in that: the amino acid fertilizer that described fertilizer carrier is rapeseed cake enzymolysis mixes with mass ratio 1:1 with cattle manure, containing organic matter 33.8%, aminoacid 4.3%, N4.20%, P2O52.26%, K2O1.08%.
3. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. according to claim 1, it is characterised in that: described vibration, centrifugal condition be: 30 DEG C, vibrate 48h, fermentation liquid 6000 × g of 170rpm be centrifuged 10min.
4. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. according to claim 1, it is characterised in that: regulating fertilizer water content is 40%.
5. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. according to claim 1, it is characterised in that: fermentation time is 5-6 days.
6. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. according to claim 1, it is characterised in that: the thalline quantity after fermentation is 108cfug-1Above.
7. the special growth-promoting biological organic fertilizer of Nicotiana tabacum L. as described in as arbitrary in claim 1-6 is in tobacco in seedling stage and the application after transplanting.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106431659A (en) * | 2016-09-28 | 2017-02-22 | 中国烟草总公司河南省公司 | Preparation method for bio-organic fertilizer special for promoting growth and resisting disease of tobacco |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309082A (en) * | 2015-10-21 | 2016-02-10 | 贵州省烟草科学研究院 | Comprehensive preventing and controlling method for tobacco bacterial wilt |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105309082A (en) * | 2015-10-21 | 2016-02-10 | 贵州省烟草科学研究院 | Comprehensive preventing and controlling method for tobacco bacterial wilt |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106431659A (en) * | 2016-09-28 | 2017-02-22 | 中国烟草总公司河南省公司 | Preparation method for bio-organic fertilizer special for promoting growth and resisting disease of tobacco |
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Application publication date: 20160720 |