CN105767547B - A kind of growing and fattening pigs health-care environment protection type fermentation compound feed - Google Patents
A kind of growing and fattening pigs health-care environment protection type fermentation compound feed Download PDFInfo
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Abstract
A kind of growing and fattening pigs health-care environment protection type fermentation compound feed, object of the present invention is to design a kind of to make full use of the unconventional water resources resources such as empty grain wheat, the cotton benevolence dregs of rice, bean dregs, bean curd yellow pulp water, alfalfa meal, growing and fattening pigs immunity of organisms can be enhanced and improve meat quality, the growing and fattening pigs health-care environment protection type fermentation compound feed of the antibiotic-free of Performance of Finishing Pigs can be improved.The technical scheme is that the present invention is made of the fermenting raw materials such as water, extruding empty grain wheat, the extruding cotton benevolence dregs of rice, steam blasting bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, probiotics preparation, glucose, compound premix, alfalfa meal.The method can significantly improve growing and fattening pigs immunity of organisms, and the general level of the health is high, can significantly improve Performance of Finishing Pigs and meat quality；Unconventional resource utilization height, energy conservation and environmental protection；Antibiotic-free, feed safety are high.
Description
Technical field:
The present invention relates to mixed feed, especially a kind of growing and fattening pigs health-care environment protection type fermentation compound feed.
Background technique:
The high-quality feeds resource scarcity such as China's corn, dregs of beans, dregs of beans production relies primarily on imported soybean, to the interdependent of import
For degree up to 75% or so, fodder maize dosage accounts for 63% or more of domestic corn annual output more than 100,000,000 tons.With aquaculture and
The disparities between supply and demand of feed industry sustainable development, conventional high-quality feed resource will be further exacerbated by, feedstuff price rises steadily,
It fluctuates more frequent.China's fattening pannage is based on corn-soybean meal at present, in corn, dregs of beans price height and feed product
Impact under the low situation of profit margin, just by market.The unconventional feed such as China's cotton benevolence dregs of rice, alfalfa meal are relatively
More, the resources such as empty grain wheat, bean dregs, bean curd yellow pulp water cause the wasting of resources and environment are dirty almost without animal feed is used for
Dye.
Growing and fattening pigs (60 kilograms of the weight of growing-finishing pig~deliver for sale stage), the development characteristics of this stage pig are, bone,
Muscle growth development is gradually reduced, and fat deposition ability is strong, and pig flesh flavor substance gradually deposits；Appetite is stronger, and digestive function is prosperous
It contains, it is stronger to feed digestion ability, it is stronger to the adaptability and resistivity of extraneous adverse circumstances.Quality, the price of feed
And the deposition level of flavor substance is to determine the key factor of growing and fattening pigs culture efficiency in pork；The health status of pig is controlled with disease
Treatment expense is also to determine the importance of pig culture efficiency.Traditional fattening swine feed is with the feeding of the good proteins such as dregs of beans
The quality of mixed feed is improved based on material, largely add the feed addictives such as antibiotic, chemical synthetic drug and heavy metal with
Reach the somatotrophic effect of antibacterial.Due to the fattening swine feed that the good proteins feed resource such as dregs of beans is very nervous, traditional
Price it is higher, swine rearing benefit is not high；Antibiotic, chemical synthetic drug and heavy metal use for a long time in fattening pannage,
Making the drug resistance of pathogen constantly enhances, so as to cause effective addition of antibiotic, chemical synthetic drug and heavy metal in feed
Amount is continuously improved, and causes the autoimmunity of growing and fattening pigs low, seriously affects the performance of growing-finishing pig production performance, resists simultaneously
A large amount of uses of the feed addictives such as raw element, chemical synthetic drug and heavy metal, also cause seriously to pollute, directly to people to environment
Class health threatens.
In order to develop growing and fattening pigs health-care environment protection type fermentation compound feed, this seminar has carried out a large amount of innovation research, pays
Go out a large amount of creative work, specifically included that the selection and processing of 1. raw material: selecting the cotton benevolence that feed resource is looser
The unconventional feed such as the dregs of rice, empty grain wheat, bean dregs, bean curd yellow pulp water, alfalfa meal carry out empty grain wheat, the cotton benevolence dregs of rice swollen
Change processing, to bean dregs carry out Steam explosion treatment, bean curd yellow pulp water is spray-dried, with reduce feed anti-nutritional factors and
Improve feed digestibility；2. feed evaluation and formula: to the extruding cotton benevolence dregs of rice, extruding empty grain wheat, steam blasting
The raw materials such as bean dregs, spray-dried tofii Huang slurry carry out the feed evaluation for growing and fattening pigs, and according to feed nutrition valence
Value evaluation result and growing and fattening pigs digestive physiological characteristics tentatively draft growing and fattening pigs fermentation compound feed formula；3. fermentation parameter
Selection and optimization: according to the physicochemical property of growing and fattening pigs digestive physiological characteristics and feedstuff, it is determined that secondary anaerobic fermentation mode,
And optimize the parameters such as fermenting microbe and adding proportion, fermentation moisture content, fermentation time；4. being repeatedly detected the cooperation of fermentation front and back
The trophic level of each nutritional ingredient of feed, clearly fermentation front and back nutriment, which is full of, lacks rule；5. adjustment formula and fermentation repeatedly:
Adjustment formula and the fermentation repeatedly on the basis of nutriment before and after fermentation is full of and lacks rule, to improve fermentation compound feed to fattening
The specific aim and high efficiency of pig.Growing and fattening pigs health-care environment protection type fermentation compound feed, feedstuff type, feedstuff are matched,
The specific aim of fermentation condition and growing and fattening pigs immunity function is extremely strong, can significantly improve Performance of Finishing Pigs.
Summary of the invention:
Object of the present invention is to be directed to growing and fattening pigs development characteristics and digestive physiological characteristics, designing a kind of can make full use of empty grain small
The unconventional water resources resources such as wheat, the cotton benevolence dregs of rice, bean dregs, bean curd yellow pulp water, alfalfa meal can enhance growing and fattening pigs immunity of organisms and mention
High meat quality can improve the growing and fattening pigs health-care environment protection type fermentation compound feed of the antibiotic-free of Performance of Finishing Pigs.
The technical scheme is that a kind of growing and fattening pigs health-care environment protection type fermentation compound feed, it includes compound premix,
It is characterized by: being made of fermenting raw materials as follows by weight: water: 23.50-24.50 parts by weight, extruding empty grain wheat:
15.00-20.00 parts by weight, the extruding cotton benevolence dregs of rice: 6.00-8.00 parts by weight, steam blasting bean dregs: 5.00-10.00 parts by weight, spray
Mist dry bean curd Huang slurry: 5.00-8.00 parts by weight, polyphenols from apple pomace extract: 0.50-1.00 parts by weight, corn: 49.31-
55.22 parts by weight, probiotics preparation: 0.50-1.00 parts by weight, glucose: 0.50-1.00 parts by weight, compound premix: 4.00
Parts by weight, alfalfa meal: 1.78-4.19 parts by weight.The probiotics preparation is lactobacillus plantarum, bafillus natto, production
Protein Candida, bacillus subtilis, and combine any the two of enterococcus faecium, Rhodopseudomonas palustris and bacillus licheniformis
Kind, total viable count is up to 13,000,000,000/gram or more.The thick protein mass content of the extruding cotton benevolence dregs of rice is 47.9% or more；It is beautiful
The thick protein mass content of rice is 8.2% or more；The thick protein mass content of alfalfa meal is 14.0% or more；Glucose
Glucose quality content is 98.0% or more.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
The present invention is the growing and fattening pigs health-care environment protection type fermentation compound feed that growing and fattening pigs provide, and can significantly improve fattening pig body
Immunity, the general level of the health is high, can significantly improve Performance of Finishing Pigs and meat quality；Empty grain wheat, the cotton benevolence dregs of rice, bean dregs, beans
The unconventional resource utilization height such as rotten yellow serofluid, alfalfa meal, energy conservation and environmental protection；Antibiotic-free, feed safety are high.
Specific embodiment:
Embodiment 1
Growing and fattening pigs health-care environment protection type fermentation compound feed of the invention, is made of fermenting raw materials as follows by weight: water:
23.50kg, extruding empty grain wheat: 20.00kg, the extruding cotton benevolence dregs of rice: 6.00kg, steam blasting bean dregs: 10.00kg, spray drying
Bean curd Huang slurry: 5.00kg, polyphenols from apple pomace extract: 0.50kg, corn: 49.31kg, probiotics preparation: 0.50kg, grape
Sugar: 0.50kg, compound premix: 4.00kg, alfalfa meal: 4.19kg.
The thick protein mass content of the extruding cotton benevolence dregs of rice described in the present embodiment is 47.9%；The thick protein quality of corn
Content is 8.2%；The thick protein mass content of alfalfa meal is 14.0%；The glucose quality content of glucose is 98.0%.
The probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili, bacillus subtilis,
And enterococcus faecium, Rhodopseudomonas palustris are combined, total viable count is 13,000,000,000/gram.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
Embodiment 2
Growing and fattening pigs health-care environment protection type fermentation compound feed of the invention, is made of fermenting raw materials as follows by weight: water:
24.00kg, extruding empty grain wheat: 19.00kg, the extruding cotton benevolence dregs of rice: 6.50kg, steam blasting bean dregs: 9.00kg, spray drying beans
Rotten yellow slurry: 6.00kg, polyphenols from apple pomace extract: 0.60kg, corn: 50.87kg, probiotics preparation: 0.60kg, glucose:
0.60kg, compound premix: 4.00kg, alfalfa meal: 2.83kg.
The thick protein mass content of the extruding cotton benevolence dregs of rice described in the present embodiment is 48.1%；The thick protein quality of corn
Content is 8.6%；The thick protein mass content of alfalfa meal is 14.8%；The glucose quality content of glucose is 98.1%.
The probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili, bacillus subtilis,
And Rhodopseudomonas palustris and bacillus licheniformis are combined, total viable count is 13,500,000,000/gram.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
Embodiment 3
Growing and fattening pigs health-care environment protection type fermentation compound feed of the invention, is made of fermenting raw materials as follows by weight: water:
24.00kg, extruding empty grain wheat: 17.50kg, the extruding cotton benevolence dregs of rice: 7.00kg, steam blasting bean dregs: 7.50kg, spray drying beans
Rotten yellow slurry: 6.50kg, polyphenols from apple pomace extract: 0.75kg, corn: 52.53kg, probiotics preparation: 0.70kg, glucose:
0.70kg, compound premix: 4.00kg, alfalfa meal: 2.82kg.
The thick protein mass content of the extruding cotton benevolence dregs of rice described in the present embodiment is 48.4%；The thick protein quality of corn
Content is 8.7%；The thick protein mass content of alfalfa meal is 14.9%；The glucose quality content of glucose is 98.4%.
The probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili, bacillus subtilis,
And enterococcus faecium, bacillus licheniformis are combined, total viable count is 13,000,000,000/gram.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
Embodiment 4
Growing and fattening pigs health-care environment protection type fermentation compound feed of the invention, is made of fermenting raw materials as follows by weight: water:
24.00kg, extruding empty grain wheat: 16.00kg, the extruding cotton benevolence dregs of rice: 7.50kg, steam blasting bean dregs: 6.00kg, spray drying beans
Rotten yellow slurry: 7.00kg, polyphenols from apple pomace extract: 0.90kg, corn: 53.65kg, probiotics preparation: 0.90kg, glucose:
0.90kg, compound premix: 4.00kg, alfalfa meal: 3.15kg.
The thick protein mass content of the extruding cotton benevolence dregs of rice described in the present embodiment is 48.8%；The thick protein quality of corn
Content is 8.8%；The thick protein mass content of alfalfa meal is 15.5%；The glucose quality content of glucose is 98.5%.
The probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili, bacillus subtilis,
And enterococcus faecium, Rhodopseudomonas palustris are combined, total viable count is 13,500,000,000/gram.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
Embodiment 5
Growing and fattening pigs health-care environment protection type fermentation compound feed of the invention, is made of fermenting raw materials as follows by weight: water:
24.50kg, extruding empty grain wheat: 15.00kg, the extruding cotton benevolence dregs of rice: 8.00kg, steam blasting bean dregs: 5.00kg, spray drying beans
Rotten yellow slurry: 8.00kg, polyphenols from apple pomace extract: 1.00kg, corn: 55.22kg, probiotics preparation: 1.00kg, glucose:
1.00kg, compound premix: 4.00kg, alfalfa meal: 1.78kg.
The thick protein mass content of the extruding cotton benevolence dregs of rice described in the present embodiment is 48.1%；The thick protein quality of corn
Content is 8.7%；The thick protein mass content of alfalfa meal is 14.9%；The glucose quality content of glucose is 98.1%.
The probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili, bacillus subtilis,
And enterococcus faecium, bacillus licheniformis are combined, total viable count is 14,000,000,000/gram.
The compound premix is that the dedicated antibiotic-free of growing and fattening pigs of Xinxiang City Jiao Shoukang feed corporation,Ltd production is multiple
Close premix.
The preparation method is that: (1) by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, is carried out first time anaerobic fermentation (closed), fermentation time 2 days；(5) first time anaerobism
After fermentation, fermented feed is taken out, the extruding empty grain wheat, the steam that crushed 40 mesh screens with predetermined weight part 50%
Explosion bean dregs, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) mixed
It closes uniform mixed feed to be packed into closed container or fermentation bag, carries out second of anaerobic fermentation (closed), can start after 4 days
It uses.
Effect of the invention is further illustrated below with reference to scientific experimentation.
Materials and methods
1.1 experimental designs and test Diet Formula
This test is designed using check experiment, and test group uses growing and fattening pigs health-care environment protection type fermentation compound feed of the invention
(embodiment 3), the formula that control group uses is identical as test group, azymic, while adding 20mg/kg aureomycin, each processing 6
A repetition.
Experimental animal, grouping and feeding management
It is left according to the method choice average weight 28kg of " parity is consistent, nutrition condition, weight is close, castrate male and female fifty-fifty "
Right healthy, active " Du × length × big " three way cross growing and fattening pigs 72 (each processing group original body mass difference is not significant), with
Machine is divided into 2 groups (control group and test groups), and every group 36, every group sets 6 repetitions, 6 pigs of each repetition.It is pre- after test pig grouping
It raises 1 week, to adapt to environment and feed, enters formal test in 35kg or so, test pig daily 9:00 and 16:00 are raised during test
It feeds twice, is freely eaten and drinking-water, by pig farm normal procedure expelling parasite, immunity inoculation, disinfection and sanitation and hygiene are observed before feeding and educated
The diarrhea and health status of hog.
Testing index and method
1.3.1 production performance index and method
On-test and reach 100kg weight when, test pig on an empty stomach weigh, each duplicate feeding of accurate recording during test
Amount.
Calculate each group average daily gain, average daily gain and feedstuff-meat ratio.
Meat guality measurement
After feeding experiment, each group test pig freely supplies water, and conventional butcher and trunk is all carried out after fasting 16 hours
Segmentation removes pig carcass eye muscle (left side) to measure following parameter:
1.3.2.1 yellowish pink measurement
Test pig takes eye muscle chest section meat sample to be stored in refrigerator after butchering, save 24 hours under the conditions of 0-4 DEG C, meat sample is cut
It opens, meat sample thickness is no less than 1.5 centimetres, then stands under the conditions of covering oxygen flow film at 1 DEG C 3 DEG C of scholar on the fresh section of meat sample
1 hour, colorimetric scoring was carried out with NPPC color board made in U.S.A:
Standards of grading, 1 point=canescence (abnormal yellowish pink)；2 points=slight greyish white (tendency is abnormal yellowish pink)；3 points=normal scarlet
Color；4 points=slightly peony (belong to normal yellowish pink)；5 points=mulberry (abnormal yellowish pink).
The measurement of meat sample pH value
Eye muscle chest section is completely exfoliated in test pig after butchering, after meat sample is stored 24 hours in 0-4 DEG C of refrigerator, by eye muscle middle section
An osculum is opened on surface, and the electrode of PHS-25 type digital display acidometer is inserted directly into the slurries that this mouthful touches muscle exudation, reads PH
The numerical value shown on meter, is accurate to 0.01 or more, is expressed as PH24.
Waterpower measures
Using Kauffman method, also known as fast grade filter paper method, it is close to meat sample with filter paper (Schleicher&Schuell-589)
Filter Paper Dry wetted surface is observed on surface.
It is waterpower standards of grading, 1 point=filter paper, 20% area is saturated；2 points=filter paper, 40% area is saturated；3 points=filter paper
60% area is saturated；4 points=filter paper, 80% area is saturated；5 points=filter paper, 100% area is saturated.
Tender assay
The hot fresh sample 500g of trunk eye muscle chest section, after curing, 78-80 DEG C of water-bath, with the sampler of 1.27cm diameter along flesh fibre
Dimension trend cuts 2.5 cubic centimetres of sample prescription cutlet 10, measures tenderness with C-LM3 type digital display Meat Tenderness instrument.
The measurement of flavor substance inosinicacid
A. reagent 1M perchloric acid；1M saleratus；Standard specimen: hypoxanthine (Sigma, 99 % of purity)；Sodium guanylate
(Sigma, purity > 99 %)；Inosine acid disodium (Sigma, purity > 99 %)；Inosine (Sigma, purity 99%)；Adenosine monophosphate
(Sigma, purity 99%)；Adenosine diphosphate (ADP) (Sigma, purity %%)；Adenosine disodium triphosphate (Sigma, 99 % of purity).
B. electronics balance；50ml plastic centrifuge tube；High performance liquid chromatograph includes LC-6A pump；CTO-6A thermocolumn
Case；SPD-6AV ultraviolet-visible detector；SIL-6A autosampler；SCL-6A central controller；C-R6A data processing system
System；Glass homogenizer；Liquid-transfering gun；50 ml volumetric flasks；Centrifuge；Eddy mixer.
C. chromatographic condition chromatographic column: 5 μm of 150 × 4.6 mm of Diamonsil C18；Column temperature: 40 DEG C；Mobile phase: 100
MM phosphoric acid (adjusts PH 6.8 with triethylamine): acetonitrile=100:1；Flow velocity: 1.0 ml/min；Wavelength: 254 nm of UV；Sample volume:
5μ1。
D. sample treatment takes out the meat sample of freezing, accurately weighs fresh meat sample 1g or so, is placed in homogenate tube, adds 5 ml
1M perchloric acid solution, even pulp nucleotide extracting solution, extracting solution are transferred in 50 ml centrifuge tubes, then with 20 ml 1M high chlorine
Sour washing homogenate tube by several times, is poured into centrifuge tube.5 000 rpm are centrifuged 10 min, and supernatant is transferred in 50 ml volumetric flasks,
Precipitating is washed (dispersed residue with ultrasonic oscillator) in two times with 25 ml 1M perchloric acid again, and after centrifugation, supernatant turns together
Enter in 50 ml volumetric flasks, is shaken up with 1M perchloric acid constant volume, obtain nucleotide extracting solution.2.5 ml extracting solutions are drawn, 1M is used
KHCO3 is neutralized to pH 6.5.15000 rpm are centrifuged 10 min, draw machine measurement on 5 μ 1.
Data processing and statistical method
As unit of test process, T inspection is carried out with the ttest process of SAS statistical software.As a result average value ± standard is used
Difference indicates.
Test result
2.1 production performance
Production performance test result is shown in Table 1.Compared with the control group, test group average daily gain improve 13.15%(P <
0.05)；Feed-weight ratio improves 4.96%(P < 0.05)；Average daily gain improves 7.54%(P < 0.05).Test result
Show that growing and fattening pigs health-care environment protection type fermentation compound feed of the invention can substitute the fattening swine feed containing antibiotic,
The average daily gain and average daily gain that growing and fattening pigs can be significantly improved significantly improve feed-weight ratio, improve feed efficiency.
1 Performance of Finishing Pigs test result of table
| Project | Control group | Test group |
| Average daily gain (kg) | 0.631±0.039* | 0.714±0.041* |
| Average daily gain (kg) | 1.65±0.06* | 1.78±0.04* |
| Feed-weight ratio | 2.62±0.02* | 2.49±0.06* |
Note: in table go together data shoulder be marked with * person's significant difference (P < 0.05), similarly hereinafter.
2.2 meat guality
Meat guality test result is shown in Table 2.Compared with the control group, the Color Score of test group improve 17.39%(P <
0.05), test group is that waterpower improves 45.95%(P < 0.05), the tenderness of test group improves 6.02%(P < 0.05)；
Meat sample pH value difference is not significant (P > 0.05).Test result shows that growing and fattening pigs health-care environment protection type fermentation cooperation of the invention is raised
Material can substitute the fattening swine feed containing antibiotic, and can significantly improve fattening pig muscle is waterpower, yellowish pink and tenderness,
Meat sample pH value is not significantly different.
2 meat guality test result of table
| Group | Control group | Test group |
| Color Score (divides) | 2.76±0.25* | 3.24±0.18* |
| Meat sample pH value | 5.74±0.14 | 5.92±0.16 |
| It is waterpower (%) | 5.68±1.29* | 3.07±0.72* |
| Tenderness (newton) | 42.84±1.21* | 40.26±1.19* |
2.3 inosine acid contents
Inosine acid content test result is shown in Table 3.Compared with the control group, the inosine acid content of test group improves 21.79%(P
< 0.05).Test result shows that growing and fattening pigs health-care environment protection type fermentation compound feed of the invention can be substituted containing antibiotic
Swine feed is fattened, the content of inosinicacid in pork can be significantly improved, improve pig flesh flavor.
3 inosinicacid content's index test result of table
| Project | Control group | Test group |
| Inosinicacid (the fresh sample of mg/g) | 2.34±0.27* | 2.85±0.19* |
Claims (1)
1. a kind of growing and fattening pigs health-care environment protection type fermentation compound feed, it includes compound premix, it is characterised in that: by as follows by weight
The fermenting raw materials of amount part meter are made: water: 23.50-24.50 parts by weight, extruding empty grain wheat: 15.00-20.00 parts by weight, extruding
The cotton benevolence dregs of rice: 6.00-8.00 parts by weight, steam blasting bean dregs: 5.00-10.00 parts by weight, spray-dried tofii Huang slurry: 5.00-
8.00 parts by weight, polyphenols from apple pomace extract: 0.50-1.00 parts by weight, corn: 49.31-55.22 parts by weight, probiotics system
Agent: 0.50-1.00 parts by weight, glucose: 0.50-1.00 parts by weight, compound premix: 4.00 parts by weight, alfalfa meal:
1.78-4.19 parts by weight；The thick protein mass content of the extruding cotton benevolence dregs of rice is 47.9% or more；The thick protein of corn
Mass content is 8.2% or more；The thick protein mass content of alfalfa meal is 14.0% or more；The glucose quality of glucose contains
Amount is 98.0% or more；Probiotics preparation be lactobacillus plantarum, bafillus natto, candida utili and bacillus subtilis,
And any two in three kinds in combination enterococcus faecium, Rhodopseudomonas palustris and bacillus licheniformis；Growing and fattening pigs health ring
The preparation method of guarantor's type fermentation compound feed, (1) is by the water of predetermined weight part, glucose, probiotics preparation and crushed 60 mesh
The extruding cotton benevolence dregs of rice of sieve pour into bucket, mix thoroughly, 37 DEG C isothermal vibration culture 4 hours, carry out the activation of probiotics preparation, be fabricated to
Actication of culture liquid；(2) compound premix of predetermined weight part is added in actication of culture liquid and is pre-mixed, be fabricated to premix
Close object；(3) by the extruding empty grain wheat that crushed 40 mesh screens of predetermined weight part 50%, steam blasting bean dregs, spray drying beans
Rotten Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal and premix are uniformly mixed；(4) uniformly mixed feeding
Material is packed into closed container or fermentation bag, progress first time anaerobic fermentation, and fermentation time 2 days；(5) first time anaerobic fermentation knot
Shu Hou takes out fermented feed, the extruding empty grain wheat, the steam blasting beans that crushed 40 mesh screens with predetermined weight part 50%
Slag, spray-dried tofii Huang slurry, polyphenols from apple pomace extract, corn, alfalfa meal are uniformly mixed；(6) uniformly mixed
Mixed feed be packed into closed container or fermentation bag, carry out second of anaerobic fermentation, after 4 days complete preparation.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101683125A (en) * | 2008-09-28 | 2010-03-31 | 天津生机集团股份有限公司 | Method for preparing biological fermentation feed for pigs |
| CN102160599A (en) * | 2011-03-07 | 2011-08-24 | 顾建洪 | Biologically-fermented complete compound feed |
| CN102613428A (en) * | 2012-04-20 | 2012-08-01 | 湖南九鼎科技（集团）有限公司 | Probiotics fermented feed and preparation method thereof |
| CN103652396A (en) * | 2013-10-12 | 2014-03-26 | 周天林 | Pig feed prepared by fermenting apple pomace and preparation method of pig feed |
| CN103829091A (en) * | 2014-03-04 | 2014-06-04 | 河南科技学院 | Mixed meal type fermented and concentrated feed for fattening pigs |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101683125A (en) * | 2008-09-28 | 2010-03-31 | 天津生机集团股份有限公司 | Method for preparing biological fermentation feed for pigs |
| CN102160599A (en) * | 2011-03-07 | 2011-08-24 | 顾建洪 | Biologically-fermented complete compound feed |
| CN102613428A (en) * | 2012-04-20 | 2012-08-01 | 湖南九鼎科技（集团）有限公司 | Probiotics fermented feed and preparation method thereof |
| CN103652396A (en) * | 2013-10-12 | 2014-03-26 | 周天林 | Pig feed prepared by fermenting apple pomace and preparation method of pig feed |
| CN103829091A (en) * | 2014-03-04 | 2014-06-04 | 河南科技学院 | Mixed meal type fermented and concentrated feed for fattening pigs |
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