CN105766947A - Lepidoptera insect insecticide synergist - Google Patents
Lepidoptera insect insecticide synergist Download PDFInfo
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- CN105766947A CN105766947A CN201610157884.0A CN201610157884A CN105766947A CN 105766947 A CN105766947 A CN 105766947A CN 201610157884 A CN201610157884 A CN 201610157884A CN 105766947 A CN105766947 A CN 105766947A
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- insecticide
- terpinene
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
- A01N37/28—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof containing the group; Thio analogues thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing acyclic or cycloaliphatic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/14—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing aromatic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- Agronomy & Crop Science (AREA)
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- Health & Medical Sciences (AREA)
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Abstract
The invention discloses a lepidoptera insect insecticide synergist. Active ingredients of the lepidoptera insect insecticide synergist include iso-ascaridole, alpha-terpinene and germacrene B, a mass concentration ratio of the iso-ascaridole to the alpha-terpinene to the germacrene B in a solvent is 1-20:1-10:1-15, and the active ingredients can be extracted from dysphania ambrosioides or chenopodium aristatum. When the insecticide synergist is used for killing lepidoptera insects, a volume percentage concentration of the iso-ascaridol, the alpha-terpinene and the germacrene B in an insecticide is 1-15%. According to pharmacodynamic tests, insecticide efficacies can be remarkably improved by consumption of a small quantity of the lepidoptera insect insecticide synergist, and insecticide consumption can be reduced by 20-45% under equal control effects, so that insecticide consumption is effectively reduced, drug resistance of harmful organisms is overcome and weakened, environment pollution is reduced, and remarkable economic benefit and environment protection significances are achieved.
Description
Technical field
The invention belongs to pesticide synergistic agent technical field, be specifically related to a kind of lepidopterous insects insecticidal synergist.
Background technology
At present, insecticide is still that agricultural controls the main method that insect is caused harm, and unreasonable yet with insecticide makes
With, cause 3R(resistance, agriculture residual, rampant again) problem.More than 50 kinds of insecticides are created resistance by such as diamondback moth, even if
Thought that being not likely to produce the benzoyl group Thiourea of resistance, insect growth regulator, IGR class, pleocidin and Bt etc. the most successively produced in the past
Give birth to resistance.The generation of pest resistance to insecticide adds the control difficulty of insect, and conventional countermeasure is for strengthening field pesticides
Consumption.But a large amount of uses of agricultural chemicals are the emphasis sources of the series of problems such as person poultry poisoning, residues of pesticides, also make many next simultaneously
The pesticide new variety being difficult to lose drug effect.Using synergist is one of important method improving insecticide drug effect, for delaying
Pest resistance to insecticide also functions to important function.But, pesticide synergistic agent, surfactant, pesticide solvent and filling carrier are to lactation
Animal, health and plant all have certain toxic action, cause the concern of people very early, until the later stage eighties
(1989) and the beginning of the nineties (1992) insecticides adjuvant and application International Academic Conference hold after people the most really start to recognize agriculture
The harmfulness of medicine auxiliary agent.At present, use more than 1200 kind auxiliary agents are divided into four by toxicity and degree of danger by EPA (EPA)
Class is managed, and associated agricultural product proposes harsh import standard.Therefore, research and development meet environmental protection, health, continue
The pesticide synergistic agent of idea of development has great importance.
Genus Chenopodium, is distributed in all over the world by about 250 kinds, and China has 19 kinds, and various places are all produced.Thorn lamb's-quarters and chenopodium ambrosiodies are
Chenopodiaceae (Chenopodiaceas) genus Chenopodium.Thorn lamb's-quarters, stem is upright, and multi-branched has striped, and herb sallow is to yellow green, after autumn
Often band aubergine, herb gathered in summer, autumn.Clean, dry, have medical value, as invigorated blood circulation;Dispelling wind and arresting itching.Dysmenorrhoea;Amenorrhoea;Cross
Quick property dermatitis and measles;Chenopodium ambrosiodies (Chenopodium ambrosioides L.), China is commonly called as stinkweed, desinsection mustard, Artemisia santonica
Deng, have the highest medical value (Wang Jinhong, Chen Renshou. clinical practice Chinese medicine dictionary [M]. Beijing: Golden Shield publishing house, 2003,
40-41.;Singh H P, Batish D R, Yadav S, et al. Chemical composition of
essential oil from leaves of Chenopodium ambrosioides L. from Chandigarh,
India [J]. Chemistry of Natural Compounds, 2008, 44(3): 378-379;China Medicine University.
The Chinese medicine Ci hai first volume [M]. Beijing: China Medical Science Press, 1993,1:232.).In China, chenopodium ambrosiodies is mainly distributed
In Fujian, Jiangxi, Zhejiang, Jiangsu, Guangdong, Guangxi, the province such as Taiwan (Huang Xue peak, Li Fan, Kong Lingyi etc. chenopodium ambrosiodies chemistry becomes
Point research [J]. China's natural drug, 2003,5(1): 24-26) it is considered to be a kind of Strategy of Alien Invasive Species.
Some kinds of genus Chenopodium have enrichment to heavy metal in soil.Insect is found by some compositions in thorn lamb's-quarters
Spouse, the behavior such as take food also have certain interference and Repellent effects.And during the powder of chenopodium ambrosiodies leaf and essential oil are to storing away grain
Six kinds of beetles have control action (Tapondjou L A, Adler C, Fontem D A, et al. Efficacy of
powder and essential oil from Chenopodium ambrosioides L. leaves as post-
harvest grain protectants against six-stored product beetles [J]. Journal of
Stored Products Research, 2002, 38: 395-402.).Chenopodium ambrosiodies essential oil has poisoning, food refusal to cabbage caterpillar
Want with the biological know-how such as growth inhibition (Li Bing, Wei Hui, topaz is clear. the supercritical CO of chenopodium ambrosiodies essential oil2Extraction conditions and desinsection
Main Primary Study [J]. East China insect journal, 2006,15(4): 305-308. topaz is clear, Wei Hui, accounts for will hero etc. soil chaste tree
Mustard essential oil is to cabbage caterpillar poisoning, food refusal and the research [J] of growth inhibition effect. Fujian Journal of Agricultural Sciench, and 2007,22(3): 266-
270.), alkaloid housefly is had toxic action (Zhao Jianwei, Wei Hui account for will male etc. the chenopodium ambrosiodies biology total alkali poison to housefly
The effect of killing and the impact [J] of insecticide sensitivity. East China insect journal, 2007,16(1): 64-69), essential oil pretreatment can affect little
Diamond-back moth larva to the insecticide sensitivity of ethiprole (Sun Hongxia, Wei Hui account for will male etc. diamondback moth children after the pretreatment of chenopodium ambrosiodies essential oil
The worm change [J] to fipronil susceptibility. East China insect journal, 2008,17(1): 9-13).Chenopodium ambrosiodies plant can suppress weeds
Growth (Jim é nez-Osornio F M V Z J, Kumamoto J, Wasser C. Allelopathic activity
of Chenopodium ambrosioides L. [J]. Biochemical Systematics Ecology, 1996, 24
(3): 195-205.)
Isolated different fennel element, α-terpinene and the germacrene B of driving is extracted for insecticide synergy from chenopodium ambrosiodies or thorn lamb's-quarters
Agent, effect is obvious, it is possible to decrease Pesticide use amount, has important economical and environmentally friendly meaning.
Summary of the invention:
It is an object of the invention to provide a kind of lepidopterous insects insecticidal synergist.The active component of this synergist is different to drive fennel
Element, α-terpinene and germacrene B.The test of pesticide effectiveness proves, uses the synergist of a small amount of present invention to be remarkably improved agricultural chemicals medicine
Effect, can reduce insecticide consumption 20%-45% under equal preventive effect, effectively reduce pesticide usage amount, overcome and delay harmful organism
The resistance to the action of a drug, reduce environmental pollution, there is significant economic benefit and environmental protection meaning.
For achieving the above object, the present invention adopts the following technical scheme that
A kind of lepidopterous insects insecticidal synergist, its active component is driven fennel element, α-terpinene and germacrene B group by different
Becoming, mass concentration ratio in a solvent is: 1 ~ 20:1 ~ 10:1 ~ 15.
Preferably, described lepidopterous insects insecticidal synergist, its active component is driven fennel element, α-terpinene and big by different
Root myrcene B forms, and mass concentration ratio in a solvent is: 10:5:10.
Described solvent is atoleine.
Described different fennel element, α-terpinene and the germacrene B of driving is to extract to obtain from Chenopodium chenopod.
Described different fennel element, α-terpinene and the germacrene B of driving is to extract to obtain from chenopodium ambrosiodies or thorn lamb's-quarters.
The application in lepidopteran insecticidal agent of a kind of synergist as above: poisoning lepidopterous insects time, different drive fennel element,
α-terpinene and germacrene B concentration expressed in percentage by volume in insecticide are 1%-15%.
The targeted lepidopterous insects of the present invention is diamondback moth, prodenia litura, beet armyworm, cabbage caterpillar and mango horizontal line tail
One in noctuid.
When using described insecticidal synergist to act on lepidopterous insects, different drive fennel element, α-terpinene and germacrene B
The concentration expressed in percentage by volume shared in insecticide of the mixed thing formed is 1%-15%, concretely 10%.
The remarkable advantage of the present invention is:
The present invention by isolated different drive fennel element, α-terpinene and germacrene B mixture after, produce synergistic function, add
Enter the insecticidal action that can effectively strengthen agricultural chemicals in insecticide;Insecticide consumption 20%-45% can be reduced, effectively under equal preventive effect
Reduce effective dose, overcome and delay the resistance to the action of a drug of harmful organism, reduce environmental pollution, for other active component in essential oil
Function Identification there is important reference.
Detailed description of the invention
Present invention the following example further illustrates the present invention, but protection scope of the present invention is not limited to following reality
Execute example.
One, beet armyworm drives fennel element, α-terpinene and the behavior reaction of germacrene B compound to different
Y-shaped smell sensing apparatus is used to measure the beet armyworm behavior reaction to compound (by this laboratory according to olfactometer general principle
Improving, granted patent number is: ZL201020239767.7).Y-piece principal arm 20cm, two arm lengths are respectively 10cm, and two-arm is pressed from both sides
90 DEG C of angle.Two-arm pipe end silicone tube sequentially connects distilled water bottle and activated carbon bottle, and last activated carbon bottle is opened in air, elder brother
It is connected in air extractor with gauze at worm release bottle.Filter paper bar is cut into 5cm × 1cm, is converted into " V " shape, be placed in trap entrance glass
Guan Zhong.(compound shared volumetric concentration in a solvent is 25% to take 100 these compounds of μ l respectively;Different drive fennel element, α-terpinene and
The mass ratio of germacrene B is: 10:5:10) uniformly dripping in the taste source bottle put on filter paper bar, another side has to drip
The filter paper of the atoleine of 100 μ l is 1L/min as reference, pumping airflow flow velocity, respectively enters the air-flow of two pipes through making a living
Property charcoal filter and distilled water humidify.Take 2 age in days beet armyworm adults to be placed in insect release bottle, be 20 every time, separately measure,
Be repeated 5 times, surveyed every time and i.e. dipped washes of absolute alcohol Y tube inside and outside wall with cotton balls and dry, then exchange Y tube two-arm and
The bottle comparison of taste source, to eliminate the impact that beet armyworm behavior is likely to result in by the geometric error of two-arm.Examination worm is put into Y tube
Starting test in insect release bottle, statistical experiment result after 2 hours, the beet armyworm rested on Y tube principal arm all regards
For reactionless.Result shows, different fennel element, α-terpinene and the germacrene B compound of driving of this experimental formula is to beet armyworm
Adult creates Repellent effects, and processing end does not has beet armyworm;Compare end and be then 14.11 ± 5.23.
Two, diamondback moth is to the anti-food rate containing the different blade driving fennel element, α-terpinene and germacrene B compound
Prepare cabbage leaves with card punch (diameter 1.40cm), be immersed in the compound that mass concentration is 27 μ g/ml and (different drive fennel
The mass ratio of element, α-terpinene and germacrene B is: 10:5:10), takes out and dries, put people and be lined with the training of moisturizing filter paper after 5s
Supporting in ware (diameter 9.0cm), 4, blade put by every ware, accesses diamondback moth 2 instar larvae 1 of hungry 4h, each process 10
Head examination worm, is repeated 3 times.The comparison blade clear water of the acetone containing same concentrations and Tween 80 processes, respectively at meeting worm 24 h
Take pictures with Sony digital camera with after 48 h, with the 1 square scraps of paper of cm for shooting comparison, with the selection instrument of Photoshop software
Touch off feeding site, calculate according to pixel and take food area, calculate its anti-food rate according to following formula, by Finney probit analysis
Try to achieve concentration AFC in linear regression formula and food refusal.
Anti-food rate %=(control group food ingestion-process group food ingestion)/control group food ingestion × 100.
Leaf dish is after this compound processes, from the point of view of the food ingestion of 24h, 36h and 48h of diamondback moth 2 instar larvae and anti-food rate,
The average food ingestion of 24h is 294.11 ± 35.23mm2Head-1, average anti-food rate is the average food ingestion of (67.39 ± 9.46) %, 36h
It is 201.52 ± 30.53mm2Head-1, average anti-food rate is that the average food ingestion of (64.57 ± 8.49) %, 48h is then respectively 85.46
±19.37mm2Head-1, average anti-food rate (60.47 ± 8.47) %.It is 698.26 ± 98.39 that 24h compares average food ingestion
mm2Head-1, it is 1100.32 ± 124.23 mm that 48h compares average food ingestion2Head-1.All show higher in 24h and 48h
Anti-food rate.
Three, synergist application in lepidopterous insects insecticide
Different fennel element, α-terpinene and the germacrene B of driving extracting separation in chenopodium ambrosiodies is entered according to mass concentration ratio 10:5:10
Row mixture, the synergist taking 1mL carries out mixture with 9mL insecticide, kills lepidopterous insects, statistical results.Will
Synergist uses after mixing with different types of insecticide in proportion, and using effect is as shown in table 1.
Table 1 synergist insecticide dissimilar with lepidopterous insects mix after effectiveness comparison
As shown in data in table, the different fennel element that drives of the present invention is remarkably improved pesticide efficacy as insecticidal synergist, on an equal basis
Insecticide consumption 20%-45% can be reduced under preventive effect, effectively reduce effective dose, overcome and delay the resistance to the action of a drug of harmful organism.
Claims (6)
1. a lepidopterous insects insecticidal synergist, it is characterised in that: its active component is driven fennel element, α-terpinene and big by different
Root myrcene B forms, and mass concentration ratio in a solvent is: 1 ~ 20:1 ~ 10:1 ~ 15.
Lepidopterous insects insecticidal synergist the most according to claim 1, it is characterised in that: its active component is driven fennel by different
Element, α-terpinene and germacrene B composition, mass concentration ratio in a solvent is: 10:5:10.
Lepidopterous insects insecticidal synergist the most according to claim 1 and 2, it is characterised in that: described solvent is liquid
Paraffin body.
Lepidopterous insects insecticidal synergist the most according to claim 1 and 2, it is characterised in that: described different drive fennel element, α-
Terpinene and germacrene B are to extract to obtain from Chenopodium chenopod.
Lepidopterous insects insecticidal synergist the most according to claim 4, it is characterised in that: described different drive fennel element, α-
Terpinene and germacrene B are to extract to obtain from chenopodium ambrosiodies or thorn lamb's-quarters.
6. a synergist as claimed in claim 1 or 2 application in lepidopteran insecticidal agent, it is characterised in that: add to
Time in insecticide, different fennel element, α-terpinene and the germacrene B concentration expressed in percentage by volume in insecticide of driving is 1%-15%.
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CN108576065A (en) * | 2018-04-11 | 2018-09-28 | 福建省农业科学院植物保护研究所 | A kind of Pesticidal combination containing chenopodium ambrosiodies essential oil and Tea Saponin |
Citations (1)
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CN101569317A (en) * | 2009-06-11 | 2009-11-04 | 福建省农业科学院植物保护研究所 | Chlorpyrifos microemulsion containing chenopodium ambrosioides oil and preparation method thereof |
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CN101569317A (en) * | 2009-06-11 | 2009-11-04 | 福建省农业科学院植物保护研究所 | Chlorpyrifos microemulsion containing chenopodium ambrosioides oil and preparation method thereof |
Non-Patent Citations (2)
Title |
---|
赵中振等主编: "《当代药用植物典 第4册》", 30 June 2008 * |
赵建伟: "土荆芥精油对杀虫剂渗透促进作用的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
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CN108576065A (en) * | 2018-04-11 | 2018-09-28 | 福建省农业科学院植物保护研究所 | A kind of Pesticidal combination containing chenopodium ambrosiodies essential oil and Tea Saponin |
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