CN105758542A - Improved cell temperature sensor and manufacturing method thereof - Google Patents
Improved cell temperature sensor and manufacturing method thereof Download PDFInfo
- Publication number
- CN105758542A CN105758542A CN201610241330.9A CN201610241330A CN105758542A CN 105758542 A CN105758542 A CN 105758542A CN 201610241330 A CN201610241330 A CN 201610241330A CN 105758542 A CN105758542 A CN 105758542A
- Authority
- CN
- China
- Prior art keywords
- glass
- nano
- micropipe
- cell temperature
- composite material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
- G01K7/01—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions
- G01K7/015—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions using microstructures, e.g. made of silicon
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K2211/00—Thermometers based on nanotechnology
Abstract
The invention relates to an improved cell temperature sensor and a manufacturing method thereof. The improved cell temperature sensor includes two glass micropipettes, wherein the two glass micropipettes are bonded together in parallel; each glass micropipette is respectively filled with different conductive composite materials; the conductive composite materials are flowed out from the front port of each glass micropipette and then are mutually integrated and congealed into a heterojunction; and each glass micropipette is provided with a conductor being connected with the conductive composite material. The improved cell temperature sensor is reasonable in design and simple in structure, can conveniently measure the temperature in a unicell or measure the temperature distribution on the surface of a cell, and has the advantages of being easy to draw materials, being simple and convenient to manufacture, and being conductive to improvement of the efficiency of the related scientific research.
Description
Technical field
The present invention relates to a kind of scientific research device, especially a kind of adopt metal material and that chemical polymerization thing is made, cell can be carried out the sensor of thermometric, cell temperature transducer of a kind of improvement specifically and preparation method thereof.
Background technology
Along with the development of life sciences and New Sensing Technology, life science can be focused on cell dimensions by people, including the temperature measuring cell.At present, industrial thermocouple is generally adopted to make cell temperature transducer, by two one metal wires by weld method link together making thermocouple cell is carried out thermometric, but, restriction due to its structure and size, this kind of sensor cannot puncture entrance cell measurement cell temperature or measure cell surface Temperature Distribution, accordingly, it would be desirable to design a kind of cell temperature transducer that can overcome the problems referred to above.
Summary of the invention
It is an object of the invention to for the cell thermometric problem currently run in life science field, cell temperature transducer that a kind of improvement is provided and preparation method thereof, intracellular temperature can be convenient for measuring or measure cell surface Temperature Distribution, and, draw materials easily, simple for production, the efficiency of related science research can be improved.
The technical scheme is that
The cell temperature transducer of a kind of improvement, including two glass-micropipes, described two glass-micropipes are bonded together side by side；Irrigate different conducing composite materials in every glass-micropipe respectively, and mutually merge after going out from front end port flow and condense into hetero-junctions；It is respectively equipped with, in described every glass-micropipe, the conductor being connected with conducing composite material.
Further, the front end of described every glass-micropipe is semi-conical, and two glass-micropipe front-end port mutually against.
Further, described conducing composite material respectively Ag nano-mixture and Ni nano-mixture；Described Ag nano-mixture is mixed by Ag nano-particle and PDMS, and described Ni nano-mixture is mixed by Ni nano-particle and PDMS；The granular size of every kind of mixture is 20nm-5um.
Further, described conductor is respectively corresponding to the Ag silk of Ag nano-mixture and the Ni silk corresponding to Ni nano-mixture.
Further, the diameter of described every glass-micropipe front-end port is 0.5um-50um.
The manufacture method of a kind of cell temperature transducer, comprises the following steps:
A. Ag conducing composite material is prepared: mixed by a certain proportion of PDMS and Ag nano-particle, be stirred with de-airing mixer, then insert in ultrasonic washing unit, make Ag nano-particle fully dispersed in mixture；
B. with above-mentioned step operation method, Ni conducing composite material is prepared；
C. double; two glass-micropipe is prepared: behind placed side by side for identical for two diameters glass-micropipe and fixing two ends, it is positioned on two holders drawing instrument, every glass-micropipe is put into heater strip be heated drawing so that it is front end becomes conical, impulse-free robustness and mutually against tip；
D. with syringe pump, the Ag conducing composite material prepared in step a and b and Ni conducing composite material are injected separately in the micro-pipe of different glass in double; two glass-micropipe, and make described composite mutually be fused into hetero-junctions after going out from front end port flow；
E. respectively corresponding with Ni silk for Ag silk is inserted in two micro-pipes, and be connected formation output conductor with conducing composite material；
F. put in vacuum drying oven and heat, make conducing composite material solidify.
Further, the PDMS in described step a or b is volume ratio 7:3 with the mixed proportion of nano-particle:.
Further, the mixing time in described step a or b is 30 minutes, and the time being placed in ultrasonic washing unit is 20 minutes.
Further, the front end impulse-free robustness in described step c is most advanced and sophisticated less than 2um.
Further, the diameter of the Ag silk in described step e or Ni silk is 0.3mm.
Beneficial effects of the present invention:
The present invention is reasonable in design, simple in construction, it is possible to is convenient for measuring unicellular interior temperature or measures cell surface Temperature Distribution, and, draw materials easily, simple for production, be conducive to improving the efficiency of related science research.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Wherein: 1-glass-micropipe；2-Ag nano-mixture；3-conductor；4-Ni nano-mixture；5-hetero-junctions.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further illustrated.
As shown in Figure 1.
The cell temperature transducer of a kind of improvement, including two glass-micropipes 1, described two glass-micropipes 1 are bonded together side by side.Irrigate different conducing composite materials in every glass-micropipe 1 respectively, and mutually merge after going out from front end port flow and condense into hetero-junctions 5.According to Seebeck effect, the absolute thermo-electromotive force produced due to two kinds of metallic composites is different, voltage difference can occurs at its two ends.Further respectively have the conductor 3 being connected with conducing composite material in described every glass-micropipe 1, the voltage difference produced can be drawn, measure this voltage difference again through high-accuracy voltage table.Then, by setting up the functional relationship between voltage difference and temperature, this hetero-junctions can be made to possess the function of thermometric.The front end of described every glass-micropipe 1 is semi-conical, and two glass-micropipe 1 front-end port mutually against, control, to facilitate, the speed that conducing composite material therein flows out, and the formation of hetero-junctions 5.Described conducing composite material respectively Ag nano-mixture 2 and Ni nano-mixture 4.Wherein, described Ag nano-mixture 2 is by Ag nano-particle and PDMS(polydimethylsiloxane Polydimethylsiloxane) mix；Described Ni nano-mixture 4 is mixed by Ni nano-particle and PDMS.PDMS is flexible high molecular material, has good mobility under room temperature, it is simple in the micro-pipe of implantation glass.At the same time it can also be by adding certain cross-linking agent so that it is solidify at a certain temperature.The granular size of described every kind of mixture is 20nm-5um, and the mixed proportion of PDMS and nano-particle is volume ratio 7:3.Described conductor 3 is respectively corresponding to the Ag silk of Ag nano-mixture 2 and the Ni silk corresponding to Ni nano-mixture 4, and its diameter is 0.3mm.Preferably, the diameter of described every glass-micropipe 1 front-end port is 0.5um-50um, it is simple to controls conducing composite material and flows out from this port, and forms the hetero-junctions of suitable size.
The manufacture method of a kind of cell temperature transducer, comprises the following steps:
A. Ag conducing composite material is prepared: mixed by a certain proportion of PDMS and Ag nano-particle, be stirred with de-airing mixer, then insert in ultrasonic washing unit, make Ag nano-particle fully dispersed in mixture；Wherein, mixed proportion is volume ratio 7:3, and the vacuum stirring time is 30 minutes, and the time being placed in ultrasonic washing unit is 20 minutes, makes nano-particle fully dispersed in the composite.
B. with above-mentioned step operation method, Ni conducing composite material is prepared；
C. double; two glass-micropipe is prepared: behind placed side by side for identical for two diameters glass-micropipe and fixing two ends, it is positioned on two holders drawing instrument, every glass-micropipe is put into heater strip be heated drawing so that it is front end becomes tip conical, carrot-free；This tip be smaller in size than 2um, and make two micro-pipes most advanced and sophisticated mutually against；
D. with syringe pump, the Ag conducing composite material prepared in step a and b and Ni conducing composite material are injected separately in the micro-pipe of different glass in double; two glass-micropipe, and make described composite mutually be fused into hetero-junctions after going out from front end port flow.During operation, glass-micropipe is placed straight down, by the microscope monitoring composite extrusion from glass-micropipe that lateral view is examined.When two kinds of composites be extruded and contact with each other formation hemispherical time, stop extrusion operation；
E. respectively corresponding with Ni silk for Ag silk is inserted in two micro-pipes, and be connected formation output conductor with conducing composite material；Wherein, the diameter of Ag silk or Ni silk is 0.3mm so that it is possess good conductivity.
F. put in vacuum drying oven and heat 30min with 120 DEG C, make the conducing composite material in glass-micropipe, and the hetero-junctions of leading section obtains fast setting.
The using method of the present invention is: be first placed in mixture of ice and water by hetero-junctions, measures its voltage difference by the high-accuracy voltage table being connected with Ag silk and Ni silk, and sets up the functional relationship of voltage and temperature.Then, place this invention on high accuracy three axle parametric controller, and observed by microscope and operate so that it is the temperature measuring head of leading section is close or punctures entrance cell, carries out temperature survey.
Part that the present invention does not relate to is all same as the prior art maybe can adopt prior art to be realized.
Claims (10)
1. the cell temperature transducer improved, including two glass-micropipes, is characterized in that described two glass-micropipes are bonded together side by side；Irrigate different conducing composite materials in every glass-micropipe respectively, and fusion condenses into hetero-junctions mutually after the outflow of front end；It is respectively equipped with, in described every glass-micropipe, the conductor being connected with conducing composite material.
2. the cell temperature transducer of improvement according to claim 1, is characterized in that the front end of described every glass-micropipe is semi-conical, and two glass-micropipe front-end port mutually against.
3. the cell temperature transducer of improvement according to claim 1, is characterized in that described conducing composite material respectively Ag nano-mixture and Ni nano-mixture；Described Ag nano-mixture is mixed by Ag nano-particle and PDMS, and described Ni nano-mixture is mixed by Ni nano-particle and PDMS；The granular size of every kind of mixture is 20nm-5um.
4. the cell temperature transducer of improvement according to claim 1, is characterized in that described conductor is respectively corresponding to the Ag silk of Ag nano-mixture and the Ni silk corresponding to Ni nano-mixture.
5. the cell temperature transducer of improvement according to claim 1, is characterized in that the diameter of described every glass-micropipe front-end port is 0.5um-50um.
6. a manufacture method for cell temperature transducer, is characterized in that comprising the following steps:
A. Ag conducing composite material is prepared: mixed by a certain proportion of PDMS and Ag nano-particle, be stirred with de-airing mixer, then insert in ultrasonic washing unit, make Ag nano-particle fully dispersed in mixture；
B. with above-mentioned step operation method, Ni conducing composite material is prepared；
C. double; two glass-micropipe is prepared: behind placed side by side for identical for two diameters glass-micropipe and fixing two ends, it is positioned on two holders drawing instrument, every glass-micropipe is put into heater strip be heated drawing so that it is front end becomes conical, impulse-free robustness and mutually against tip；
D. with syringe pump, the Ag conducing composite material prepared in step a and b and Ni conducing composite material are injected separately in the micro-pipe of different glass in double; two glass-micropipe, and make described composite mutually be fused into hetero-junctions after going out from front end port flow；
E. respectively corresponding with Ni silk for Ag silk is inserted in two micro-pipes, and be connected formation output conductor with conducing composite material；
F. put in vacuum drying oven and heat, make conducing composite material solidify.
7. the processing technology of cell temperature transducer according to claim 6, is characterized in that the mixed proportion of the PDMS in described step a or b and nano-particle is volume ratio 7:3.
8. the processing technology of cell temperature transducer according to claim 6, is characterized in that the mixing time in described step a or b is 30 minutes, and the time being placed in ultrasonic washing unit is 20 minutes.
9. the processing technology of cell temperature transducer according to claim 6, is characterized in that the front end impulse-free robustness in described step c is most advanced and sophisticated less than 2um.
10. the manufacture method of cell temperature transducer according to claim 6, is characterized in that the diameter of the Ag silk in described step e or Ni silk is 0.3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241330.9A CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610241330.9A CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105758542A true CN105758542A (en) | 2016-07-13 |
| CN105758542B CN105758542B (en) | 2018-07-13 |
Family
ID=56334029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610241330.9A Expired - Fee Related CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105758542B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109029759A (en) * | 2018-06-06 | 2018-12-18 | 东南大学 | A kind of high-precision unicellular temp measuring system in real time |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030480A (en) * | 1987-07-07 | 1989-01-18 | 浙江大学 | The preparation technology of liquid ion-exchanger composite used as acetylcholine ion-selective microelectrode |
| CN101493360A (en) * | 2009-01-05 | 2009-07-29 | 东南大学 | Thermocouple with micron or nanometer grade tip curvature radius and method for producing the same |
| CN102206419A (en) * | 2011-04-02 | 2011-10-05 | 合肥工业大学 | Sensitive material for flexible temperature sensor |
| CN202024834U (en) * | 2011-02-23 | 2011-11-02 | 肇庆爱晟电子科技有限公司 | NTC (Negative Temperature Coefficient) thermistor temperature sensor with lead wire low-temperature solidification connection |
| CN102262113A (en) * | 2011-07-27 | 2011-11-30 | 东南大学 | Multifunctional probe for unicellular detection |
| CN205593661U (en) * | 2016-04-19 | 2016-09-21 | 中国人民解放军南京军区南京总医院 | Modified cell temperature transducer |
-
2016
- 2016-04-19 CN CN201610241330.9A patent/CN105758542B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030480A (en) * | 1987-07-07 | 1989-01-18 | 浙江大学 | The preparation technology of liquid ion-exchanger composite used as acetylcholine ion-selective microelectrode |
| CN101493360A (en) * | 2009-01-05 | 2009-07-29 | 东南大学 | Thermocouple with micron or nanometer grade tip curvature radius and method for producing the same |
| CN202024834U (en) * | 2011-02-23 | 2011-11-02 | 肇庆爱晟电子科技有限公司 | NTC (Negative Temperature Coefficient) thermistor temperature sensor with lead wire low-temperature solidification connection |
| CN102206419A (en) * | 2011-04-02 | 2011-10-05 | 合肥工业大学 | Sensitive material for flexible temperature sensor |
| CN102262113A (en) * | 2011-07-27 | 2011-11-30 | 东南大学 | Multifunctional probe for unicellular detection |
| CN205593661U (en) * | 2016-04-19 | 2016-09-21 | 中国人民解放军南京军区南京总医院 | Modified cell temperature transducer |
Non-Patent Citations (1)
| Title |
|---|
| CHANGLING WANG 等: "The Role of Nanotechnology in Single-Cell Detection: A Review", 《JOURNAL OF BIOMEDICAL NANOTECHNOLOGY》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109029759A (en) * | 2018-06-06 | 2018-12-18 | 东南大学 | A kind of high-precision unicellular temp measuring system in real time |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105758542B (en) | 2018-07-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106513066B (en) | A kind of three-dimensional porous graphene micro-fluidic chip and its graphene adherence method | |
| CN107192475B (en) | Temperature sensor system based on micro-fluidic chip and preparation method | |
| CN103163384B (en) | Integral plastics conductivity sensor | |
| CN105758542A (en) | Improved cell temperature sensor and manufacturing method thereof | |
| CN209446605U (en) | A kind of liquid stream control and measuring device based on hydrogel | |
| CN108654710A (en) | A kind of micro-fluidic chip measured for solution flow rate and microbubble counts | |
| CN105675443B (en) | A kind of thin material cylinder capillary rheometer | |
| CN209451370U (en) | A kind of AC Electric Heater micro mixing device of inclined field heating | |
| Hisamoto et al. | Integration of multiple-ion-sensing on a capillary-assembled microchip | |
| CN104587930A (en) | Synthesis of metal/carbon nanotube composite nanowires and special micro/nano reactor | |
| CN105214745B (en) | A kind of micro-fluidic chip | |
| Kim et al. | A micro-aspirator chip using vacuum expanded microchannels for high-throughput mechanical characterization of biological cells | |
| CN205175737U (en) | Blood sampling external member | |
| CN205593661U (en) | Modified cell temperature transducer | |
| CN209451869U (en) | A kind of micro fluidic device for realizing multiplicity detection based on electroosmotic flow | |
| US8715558B2 (en) | Capillary electrophoresis chips | |
| Liu et al. | A rigid poly (dimethylsiloxane) sandwich electrophoresis microchip based on thin‐casting method | |
| CN110511411A (en) | A kind of preparation method of temperature sensitive flexible membrane, sensor and flexible membrane | |
| CN113042119B (en) | Annular nano-pore and preparation and test method thereof | |
| CN104226384B (en) | Liquid-discharging means of bottle | |
| TWI611185B (en) | Detection device | |
| CN206192817U (en) | Fluid density measuring device in fuse -element | |
| CN203413250U (en) | Plastic micro-channel pipeline structure and manufacturing device thereof | |
| CN106563518A (en) | Micro-fluidic chip for rapidly detecting three subtypes of bladder cancer and manufacturing method and use method thereof | |
| CN216624388U (en) | Button cell tests constant temperature equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180713 Termination date: 20190419 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |