CN105758542B - A kind of improved cell temperature transducer and preparation method thereof - Google Patents
A kind of improved cell temperature transducer and preparation method thereof Download PDFInfo
- Publication number
- CN105758542B CN105758542B CN201610241330.9A CN201610241330A CN105758542B CN 105758542 B CN105758542 B CN 105758542B CN 201610241330 A CN201610241330 A CN 201610241330A CN 105758542 B CN105758542 B CN 105758542B
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- Prior art keywords
- glass
- temperature transducer
- nano
- conducing composite
- cell temperature
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K7/00—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements
- G01K7/01—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions
- G01K7/015—Measuring temperature based on the use of electric or magnetic elements directly sensitive to heat ; Power supply therefor, e.g. using thermoelectric elements using semiconducting elements having PN junctions using microstructures, e.g. made of silicon
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K2211/00—Thermometers based on nanotechnology
Abstract
The present invention relates to a kind of improved cell temperature transducer and preparation method thereof, including two glass-micropipes, two glass-micropipes are bonded together side by side；Different conducing composite materials is perfused in every glass-micropipe respectively, and is mutually merged after going out from front end port flow and condenses into hetero-junctions；It is respectively equipped with the conductor being connected with conducing composite material in the every glass-micropipe.Reasonable design of the present invention, simple in structure, the temperature or measurement cell surface Temperature Distribution that can be convenient for measuring in unicellular, moreover, materials are easy, and it is simple for production, be conducive to the efficiency for improving related science research.
Description
Technical field
The present invention relates to a kind of scientific research device, made of especially a kind of use metal material and chemical polymerization object,
The sensor of thermometric, specifically a kind of improved cell temperature transducer and preparation method thereof can be carried out to cell.
Background technology
With the development of life science and New Sensing Technology, life science can be focused on cell by people
Scale, including measure the temperature of cell.Currently, generally use industrial thermocouple makes cell temperature transducer, i.e., by two
Wire is linked together by the method for welding makes thermocouple to carry out thermometric to cell, still, due to its structure and ruler
Very little limitation, this kind of sensor can not puncture into cell measurement cell temperature or measure cell surface Temperature Distribution, therefore, need
Design a kind of cell temperature transducer that can overcome the above problem.
Invention content
The purpose of the present invention is for the cell thermometric problem currently encountered in life science field, provide one kind
Improved cell temperature transducer and preparation method thereof can be convenient for measuring intracellular temperature or measure cell surface temperature point
Cloth, moreover, materials are easy, it is simple for production, the efficiency of related science research can be improved.
The technical scheme is that：
A kind of improved cell temperature transducer, including two glass-micropipes, two glass-micropipes are bonded in side by side
Together；Different conducing composite materials is perfused in every glass-micropipe respectively, and condensation is mutually merged after going out from front end port flow
At hetero-junctions；It is respectively equipped with the conductor being connected with conducing composite material in the every glass-micropipe.
Further, the front end of the every glass-micropipe is semi-conical, and two glass-micropipe front-end ports are mutual
It abuts.
Further, the conducing composite material is respectively Ag nano-mixtures and Ni nano-mixtures；It is Ag nanometers described
Mixture is mixed by Ag nano particles and PDMS, and the Ni nano-mixtures are mixed by Ni nano particles and PDMS；
The granular size of each mixture is 20nm-5um.
Further, the conductor is respectively corresponding to the Ag silks of Ag nano-mixtures and corresponding to Ni nano-mixtures
Ni.
Further, a diameter of 0.5um-50um of the every glass-micropipe front-end port.
A kind of production method of cell temperature transducer, includes the following steps：
A. Ag conducing composite materials are prepared：A certain proportion of PDMS is mixed with Ag nano particles, with de-airing mixer into
Row stirring, then be placed in supersonic wave cleaning machine, keep Ag nano particles fully dispersed in mixture；
B. with above-mentioned step operation method, Ni conducing composite materials are prepared；
C. double glass-micropipes are prepared：Behind the placed side by side and fixed both ends of the identical glass-micropipe of two diameters, it is positioned over
On two fixators for drawing instrument, it is put into heater strip in every glass-micropipe and carries out heating drawing, its front end is made to become circular cone
Shape, impulse- free robustness and mutually abut tip；
D. the Ag conducing composite materials and Ni conducing composite materials that are prepared in step a and b are injected separately into syringe pump double
In different glass micro-pipe in glass-micropipe, and hetero-junctions is mutually fused into after so that the composite material is gone out from front end port flow；
E. it respectively by Ag and two micro-pipes of Ni corresponding mergings, and connect to form output with conducing composite material
Conductor；
F. it is put into vacuum drying chamber and heats, conducing composite material is made to cure.
Further, the mixed proportion of the PDMS in the step a or b and nano particle is volume ratio 7:3:.
Further, the mixing time in the step a or b is 30 minutes, and the time for being placed in supersonic wave cleaning machine is 20
Minute.
Further, the front end impulse- free robustness tip in the step c is less than 2um.
Further, the Ag silks in the step e or Ni diameters are 0.3mm.
Beneficial effects of the present invention：
Reasonable design of the present invention, simple in structure, the temperature or measurement cell surface temperature that can be convenient for measuring in unicellular
Distribution, moreover, materials are easy, it is simple for production, be conducive to the efficiency for improving related science research.
Description of the drawings
Fig. 1 is the structural schematic diagram of the present invention.
Wherein：1- glass-micropipes；2-Ag nano-mixtures；3- conductors；4-Ni nano-mixtures；5- hetero-junctions.
Specific implementation mode
The present invention is further illustrated with reference to the accompanying drawings and examples.
As shown in Figure 1.
A kind of improved cell temperature transducer, including two glass-micropipes 1, two glass-micropipes 1 bond side by side
Together.Different conducing composite materials is perfused in every glass-micropipe 1 respectively, and is mutually merged after going out from front end port flow solidifying
Form hetero-junctions 5.It can be at it since the absolute thermo-electromotive force that two kinds of metallic composites generate is different according to Seebeck effect
There is voltage difference in both ends.The conductor 3 being connected with conducing composite material is further respectively had in the every glass-micropipe 1, can will be produced
Raw voltage difference is drawn, then measures the voltage difference by high-accuracy voltage table.Then, by establishing between voltage difference and temperature
Functional relation, you can the hetero-junctions is made to have the function of thermometric.The front end of the every glass-micropipe 1 is semi-conical, and two
1 front-end port of root glass-micropipe mutually abuts, to facilitate the speed and hetero-junctions that control conducing composite material outflow therein
5 formation.The conducing composite material is respectively Ag nano-mixtures 2 and Ni nano-mixtures 4.Wherein, it mixes for described Ag nanometers
Object 2 is closed by Ag nano particles and PDMS（Dimethyl silicone polymer Polydimethylsiloxane）It mixes；The Ni receives
Rice mixture 4 is mixed by Ni nano particles and PDMS.PDMS is flexible high molecular material, has preferable flowing under room temperature
Property, convenient in implantation glass micro-pipe.At the same time it can also by the way that certain crosslinking agent is added, it be made to cure at a certain temperature.Institute
The granular size for stating each mixture is 20nm-5um, and the mixed proportion of PDMS and nano particle is volume ratio 7:3.It is described
Conductor 3 is respectively the Ni silks corresponding to the Ag silks of Ag nano-mixtures 2 and corresponding to Ni nano-mixtures 4, and diameter is
0.3mm.Preferably, a diameter of 0.5um-50um of 1 front-end port of every glass-micropipe, convenient for control conducing composite material
It is flowed out from the port, and forms the hetero-junctions of suitable size.
A kind of production method of cell temperature transducer, includes the following steps：
A. Ag conducing composite materials are prepared：A certain proportion of PDMS is mixed with Ag nano particles, with de-airing mixer into
Row stirring, then be placed in supersonic wave cleaning machine, keep Ag nano particles fully dispersed in mixture；Wherein, mixed proportion is body
Product ratio 7:3, it is 30 minutes to be stirred under vacuum the time, and the time for being placed in supersonic wave cleaning machine is 20 minutes, makes nano particle compound
It is fully dispersed in object.
B. with above-mentioned step operation method, Ni conducing composite materials are prepared；
C. double glass-micropipes are prepared：Behind the placed side by side and fixed both ends of the identical glass-micropipe of two diameters, it is positioned over
On two fixators for drawing instrument, it is put into heater strip in every glass-micropipe and carries out heating drawing, its front end is made to become circular cone
Shape, carrot-free tip；The size at the tip is less than 2um, and the tip of two micro-pipes is made mutually to abut；
D. the Ag conducing composite materials and Ni conducing composite materials that are prepared in step a and b are injected separately into syringe pump double
In different glass micro-pipe in glass-micropipe, and hetero-junctions is mutually fused into after so that the composite material is gone out from front end port flow.
When operation, glass-micropipe is placed straight down, composite material squeezing from glass-micropipe is monitored by the microscope that lateral view is examined
Go out process.When two kinds of composite materials are extruded and contact with each other to form hemispherical, stop squeezing out operation；
E. it respectively by Ag and two micro-pipes of Ni corresponding mergings, and connect to form output with conducing composite material
Conductor；Wherein, Ag or Ni diameters are 0.3mm, it is made to have good conductibility.
F. it is put into vacuum drying chamber with 120 DEG C of heating 30min, makes the conducing composite material in glass-micropipe, and
The hetero-junctions of front end obtains rapid curing.
The present invention application method be：Hetero-junctions is placed in mixture of ice and water first, by being connected with Ag and Ni
High-accuracy voltage table measure its voltage difference, and establish the functional relation of voltage and temperature.Then, high-precision is placed this invention into
It in three axis control platforms, and by micro- sem observation and operates, keeps the temperature measuring head of its front end close or puncture to enter cell, into
Trip temperature measures.
Part that the present invention does not relate to is the same as those in the prior art or can be realized by using the prior art.
Claims (7)
1. a kind of production method of cell temperature transducer, it is characterized in that including the following steps：
A. Ag conducing composite materials are prepared：A certain proportion of PDMS is mixed with Ag nano particles, is stirred with de-airing mixer
It mixes, then is placed in supersonic wave cleaning machine, keep Ag nano particles fully dispersed in mixture；
B. with above-mentioned step operation method, Ni conducing composite materials are prepared；
C. double glass-micropipes are prepared：Behind the placed side by side and fixed both ends of the identical glass-micropipe of two diameters, it is positioned over drawing
On two fixators of instrument, it is put into heater strip in every glass-micropipe and carries out heating drawing, its front end is made to become cone, nothing
Burr and mutually abut tip；
D. the Ag conducing composite materials and Ni conducing composite materials that are prepared in step a and b are injected separately into double glass with syringe pump
In different glass micro-pipe in micro-pipe, and hetero-junctions is mutually fused into after so that the composite material is gone out from front end port flow；
E. it respectively by Ag and two micro-pipes of Ni corresponding mergings, and connect to form output conductor with conducing composite material；
F. it is put into vacuum drying chamber and heats, conducing composite material is made to cure.
2. the production method of cell temperature transducer according to claim 1, it is characterized in that the conducing composite material point
It Wei not Ag nano-mixtures and Ni nano-mixtures；The Ag nano-mixtures are mixed by Ag nano particles and PDMS, institute
Ni nano-mixtures are stated to be mixed by Ni nano particles and PDMS；The granular size of each mixture is 20nm-5um.
3. the production method of cell temperature transducer according to claim 1, it is characterized in that before the every glass-micropipe
Hold a diameter of 0.5um-50um of port.
4. the production method of cell temperature transducer according to claim 1, it is characterized in that in the step a or b
The mixed proportion of PDMS and nano particle is volume ratio 7:3.
5. the production method of cell temperature transducer according to claim 1, it is characterized in that stirring in the step a or b
It is 30 minutes to mix the time, and the time for being placed in supersonic wave cleaning machine is 20 minutes.
6. the production method of cell temperature transducer according to claim 1, it is characterized in that front end in the step c without
Burr tip is less than 2um.
7. the production method of cell temperature transducer according to claim 1, it is characterized in that Ag silks in the step e or
Ni diameters are 0.3mm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610241330.9A CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
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| CN201610241330.9A CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
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| CN105758542A CN105758542A (en) | 2016-07-13 |
| CN105758542B true CN105758542B (en) | 2018-07-13 |
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| CN201610241330.9A Expired - Fee Related CN105758542B (en) | 2016-04-19 | 2016-04-19 | A kind of improved cell temperature transducer and preparation method thereof |
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| CN109029759A (en) * | 2018-06-06 | 2018-12-18 | 东南大学 | A kind of high-precision unicellular temp measuring system in real time |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030480A (en) * | 1987-07-07 | 1989-01-18 | 浙江大学 | The preparation technology of liquid ion-exchanger composite used as acetylcholine ion-selective microelectrode |
| CN101493360A (en) * | 2009-01-05 | 2009-07-29 | 东南大学 | Thermocouple with micron or nanometer grade tip curvature radius and method for producing the same |
| CN102206419A (en) * | 2011-04-02 | 2011-10-05 | 合肥工业大学 | Sensitive material for flexible temperature sensor |
| CN202024834U (en) * | 2011-02-23 | 2011-11-02 | 肇庆爱晟电子科技有限公司 | NTC (Negative Temperature Coefficient) thermistor temperature sensor with lead wire low-temperature solidification connection |
| CN102262113A (en) * | 2011-07-27 | 2011-11-30 | 东南大学 | Multifunctional probe for unicellular detection |
| CN205593661U (en) * | 2016-04-19 | 2016-09-21 | 中国人民解放军南京军区南京总医院 | Modified cell temperature transducer |
-
2016
- 2016-04-19 CN CN201610241330.9A patent/CN105758542B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1030480A (en) * | 1987-07-07 | 1989-01-18 | 浙江大学 | The preparation technology of liquid ion-exchanger composite used as acetylcholine ion-selective microelectrode |
| CN101493360A (en) * | 2009-01-05 | 2009-07-29 | 东南大学 | Thermocouple with micron or nanometer grade tip curvature radius and method for producing the same |
| CN202024834U (en) * | 2011-02-23 | 2011-11-02 | 肇庆爱晟电子科技有限公司 | NTC (Negative Temperature Coefficient) thermistor temperature sensor with lead wire low-temperature solidification connection |
| CN102206419A (en) * | 2011-04-02 | 2011-10-05 | 合肥工业大学 | Sensitive material for flexible temperature sensor |
| CN102262113A (en) * | 2011-07-27 | 2011-11-30 | 东南大学 | Multifunctional probe for unicellular detection |
| CN205593661U (en) * | 2016-04-19 | 2016-09-21 | 中国人民解放军南京军区南京总医院 | Modified cell temperature transducer |
Non-Patent Citations (1)
| Title |
|---|
| The Role of Nanotechnology in Single-Cell Detection: A Review;Changling Wang 等;《JOURNAL OF BIOMEDICAL NANOTECHNOLOGY》;20141031;第10卷(第10期);2598–2619 * |
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Granted publication date: 20180713 Termination date: 20190419 |