CN105754843A - Relatively enclosed multi-thin layer adherent culture device for production of microalgae - Google Patents
Relatively enclosed multi-thin layer adherent culture device for production of microalgae Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
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Abstract
The invention discloses a relatively enclosed multi-thin layer adherent culture device for production of microalgae. The device comprises a bottom bracket, a culture channel, fluid infusion tubes and a culture medium fluid container, wherein the culture channel is fixed above the bottom bracket; the culture channel comprises at least one adherent culture unit, side walls and a bottom plate; each adherent culture unit is generally arranged in the vertical direction; a gap for sunlight penetration is arranged every two adjacent adherent culture units; the side walls, the bottom plate and the culture channel form a structure, only the upper end of which is opened; a position for the input of carbon dioxide is arranged on the culture channel; one fluid infusion tube is arranged above each adherent culture unit; the fluid infusion tubes are communicated with the culture medium fluid container; a through hole for the outflow of culture fluid is formed in the bottom plate of the culture channel; the culture medium fluid container is placed below the through hole so as to collect the culture fluid flowing down from the through hole. According to the device disclosed in the invention, the supply, for algae, of gas-state carbon dioxide is realized, and the production requirement of certain algae which only can utilize free carbon dioxide is satisfied.
Description
Technical field
The invention belongs to field of microalgae cultivation, specifically a kind of relative close type multi-thin-layer adherent culture device produced for microalgae.
Background technology
No matter being produce high value added product or energy products, the core of microalgae industry development is in that on a large scale, obtains biomass at low cost.But, current unicellular microalgae is when scale evaluation, and culture efficiency also differs greatly from industrial requirement.
Affect the main factor of micro algae growth efficiency when light-use is scale evaluation, and the raising of optical energy utilization efficiency must flow through the structural design optimization cultivating equipment and solves.No matter current microdisk electrode, be that open pond is cultivated or Photoreactor is cultivated, and its common trait is to be suspended in the suspension culture in a large amount of water body with microalgae cell.After sunlight enters microdisk electrode water body, the interception of the refraction of light, scattering and frustule makes light intensity decay along incident illumination direction occurrence index, makes water body light district present the 3rd district distribution in light supersaturation district, top layer, middle level area of illumination and low light level district of lower floor.The growth of any microalgae is required for the range of light intensity between rational Jie's microalgae compensation light intensity and saturated light intensity, and (to most of microalgae, this range of light intensity is at 20～200 μm of olphotonm2Between/s).Only within the scope of this intensity of illumination, the growth of microalgae is the fastest, and its speed of growth almost linearly increases relation with the intensity of light.Lower than compensating light intensity, the respiration consumption of microalgae, more than photosynthetic synthesis, shows as the clean consumption of Biomass；Higher than saturated light intensity then considerably beyond the ability to bear of microalgae photosynthetical system, on the one hand the growth of microalgae is produced Xanthophyll cycle, make the photosynthetical system of microalgae impaired or cause that microalgae is dead, showing as light loss on the other hand.How to solve the inhibitory action of Gao Guang and efficiently to utilize luminous energy will be realize the high efficiency key of microdisk electrode.Based on this, it is proposed that culture area dilution principle, being distributed on bigger surface by the natural sunlight on certain incident illumination area (floor space), namely the sunlight area of the actual reception of microalgae culture system is much larger than incident illumination area (floor space).
It addition, microalgae autotrophy is by the DIC (CO in the external world2) it changes into the glucose needed for own growth.Traditional microalgae cell suspension culture is by CO2Input to culture medium, a part of CO2Become HCO3 -, sub-fraction CO2Exist with free form.Some microalgae cell can utilize HCO3 -And CO2Two kinds of forms, and some microalgae cell is merely able to utilize CO2.But the CO that dissociates in a liquid2Content non-normally low, cause the DIC under-supply, the carbon sequestration growth of remote-effects microalgae cell.In microdisk electrode process, supplement a certain amount of gaseous state CO2Growth rate and the Biomass of microalgae can be effectively facilitated.
Summary of the invention
In order to solve high light to the inhibitory action of micro algae growth and the problem how efficiently to utilize luminous energy, it is an object of the invention to provide a kind of relative close type multi-thin-layer adherent culture device produced for microalgae, this culture apparatus can make full use of sunlight scattering properties, and can carry out gaseous state CO2Supplement, can be used for large-scale production micro algae biomass, it is possible to be effectively improved the Biomass yield of microalgae.
It is an object of the invention to be achieved through the following technical solutions:
A kind of relative close type multi-thin-layer adherent culture device produced for microalgae, including bottom bracket, culture tank, liquid supplementation pipe and culture medium receiver, wherein, culture tank is fixed on the top of bottom bracket, described culture tank includes at least one adhere-wall culture unit, sidewall and base plate, each adhere-wall culture unit is substantially vertically arranged, leave, between adjacent two adhere-wall culture unit, the gap that sunlight passes through, culture tank is configured to the structure of only upper end open by sidewall and base plate, and culture tank leaves the position passing into carbon dioxide；
Each adhere-wall culture unit be provided above liquid supplementation pipe, each liquid supplementation pipe all connects with culture medium receiver；Being provided with the through hole for being flowed out by culture fluid on the base plate of culture tank, culture medium receiver is positioned over the lower section of this through hole, collects the culture fluid flowed down from this through hole.
Carbon dioxide can be passed into by the upper end open of culture medium, can also be passed into by aperture reserved on sidewall, due to culture medium, there is sidewall and base plate, culture medium is surrounded the structure of relative closure, plus the density of carbon dioxide more than the density of air, carbon dioxide can be assembled in the medium；In addition, adhere-wall culture unit is substantially vertically arranged, culture fluid can be made to flow through adhere-wall culture unit from top to bottom, ensure on the basis of the algal grown on adhere-wall culture unit, it is unlikely to there is too many culture fluid, it is in leather hard, so, algae on adhere-wall culture base can touch the gaseous carbon dioxide in culture medium, and then utilize gaseous carbon dioxide, owing to the concentration of gaseous carbon dioxide is bigger, can ensure that the healthy growth of the algae only with free carbon dioxide, ensure that the growth rate of this part algae and Biomass, overcome the drawback of traditional training method.
Preferably, described through hole is positioned at the lowest point of culture medium base plate.Flowed out by described through hole after conveniently the culture fluid on culture medium base plate being assembled.
Preferably, the sidewall of described culture medium leaving the aperture passing into carbon dioxide, this aperture is connected with carbon dioxide air source.
Sidewall leaves aperture, this aperture passes into carbon dioxide, owing to the density of carbon dioxide is more than the density of air, after carbon dioxide passes into, is directly gathered in the inside of culture medium, reduces the waste of carbon dioxide.
Preferably, the height of described culture tank is 0.3-1.0m.
Preferably, 0.01-10 times that distance is adherent cell height between adjacent two adhere-wall culture unit.
It is further preferred that the height of each adherent unit is lower than the height of culture tank.
It is further preferred that the position of each adherent unit is adjustable.
Preferably, described adhere-wall culture unit includes supporting axle and flexible material, supports axle and is arranged on the upper end of culture medium, and flexible material is suspended on support axle.
It is further preferred that described flexible material is fine hair material.
Preferably, described culture tank is square structure.
The invention have the benefit that
1, the vertically arranged adhere-wall culture unit of the present invention coordinates with the culture tank of only upper end open, achieve the supply of the gaseous carbon dioxide of algae, meet some production only with the algae of free carbon dioxide, the fixed effect of DIC is better, overcomes the drawback of existing training method；
2, the adhere-wall culture unit of the present invention is vertically arranged, it is possible to understand that become to be diluted by sunlight, it is possible to be effectively improved the optical energy utilization efficiency in microdisk electrode process, increases the micro algae biomass productivity in unit are；
3, the culture tank opposing seal of the present invention, it is possible to effectively prevent the pollution of external environment；
4, the present invention is in incubation, and only liquid circulation pump needs electricity consumption, and overall required energy consumption is low.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the result schematic diagram of the embodiment of the present invention 1；
Fig. 3 is the result schematic diagram of the embodiment of the present invention 2；
Fig. 4 is the result schematic diagram of the embodiment of the present invention 3.
Wherein: 1, bottom bracket；2, culture tank；3, inlet channel；4, receiver；5, liquid circulation pump；6, liquid supplementation pipe；7, axle is supported；8, flexible material；9, bottom opening；10, lateral opening hole.
Detailed description of the invention
Below in conjunction with drawings and Examples, the invention will be further described.
As it is shown in figure 1, the present invention includes bottom bracket 1, relative close type culture tank 2, adhere-wall culture unit, liquid supplementation pipe 6 and culture medium receiver 4.Culture tank 2 (height of groove is 0.3 meter) is positioned at above bottom bracket 1, installs at least one adhere-wall culture unit in groove；Each adhere-wall culture unit be arranged over liquid supplementation pipe 6, each liquid supplementation pipe 6 is connected with described culture medium receiver 4 by inlet channel 3；Be provided with bottom opening 9 in the bottom of culture tank 2, bottom opening 9 is just placed on below culture tank 2 by receiver 4；It is provided with liquid circulation pump 6 in described culture medium receiver 4, is connected by inlet channel 3 between the liquid supplementation pipe 7 above the outlet of liquid circulation pump 6 and each adhere-wall culture unit；Liquid circulation pump 5 is the device that liquid can be driven to flow in the duct, includes but not limited to peristaltic pump, immersible pump etc.；Culture tank 2 side is provided with lateral opening hole 10, can be connected with carbon compensator, it is possible to pass into containing the air of carbon dioxide, pure carbon dioxide, flue gas, through the flue gas of pre-treatment.
Gas containing carbon dioxide can also be passed into from the upper end open of culture tank 2.
Each adhere-wall culture unit includes flexible material 8 and supports axle 7.Support axle 7 two ends and be positioned at the both sides top edge of culture tank 2, be relatively fixed；Flexible material 8 Bidirectional suspension is supporting on axle 7, vertical and ground.
Liquid supplementation pipe 6 is positioned at the top of each adherent unit, along supporting the axially arranged of axle 7, is evenly equipped with multiple hole to microalgae fluid medium low on flexible material in liquid supplementation pipe 6.The bottom of culture tank 2 is provided with a bottom opening 9, flows through the biomembranous culture medium of microalgae and imports this, flows back to the receiver 4 being placed on immediately below it.
Flexible material 8 refer to solid-state, nontoxic to microalgae cell or toxicity is slight, can be suspended on that the fine hair supporting upper and wear-resistant is inorganic or organic material, include but not limited to one or more in filter cloth or fabric web material (such as towel, lint, flocking) etc..
The adhere-wall culture element number visual gauge mould installed in culture tank 2 and floor space and set, the spacing between each adherent unit 3 is 0.01～10 times that cultivates elemental area height, and spacing is adjustable.The height of each adhere-wall culture unit 3 is slightly below the height of culture tank 2.
The operation principle of the present invention is:
This device is placed in photoenvironment, microalgae seed is inoculated on the flexible material 8 of adhere-wall culture unit, the culture medium that culture medium receiver 5 grows built with applicable microalgae algae kind.In culture tank 2, pass into gaseous carbon dioxide, open liquid circulation pump 6, regulate water pump flow velocity, make microalgae fluid medium uniformly be dripped by liquid supplementation pipe 6 and drop on the surface of flexible material 8, to keep frustule moistening and to provide the nutritional labeling of micro algae growth.The culture medium more than needed flowing through microalgae biofilm surface falls into bottom culture tank 2, imports bottom opening 9 place, flows in culture medium receiver 4.Owing to culture fluid is under adhere-wall culture unit stream, so being in half dry state cultivation conditions, algae can touch the gaseous carbon dioxide in culture tank, and then utilizes free carbon dioxide.
Embodiment 1:
Testing location is Qingdao, and the testing time is in June, 2015.Support height is 0.3 meter, and culture tank height is 0.3 meter.Select superfine fibre towel and flocking as flexible material, to support the microalgae of adhere-wall culture cell surface.Culture apparatus arranges 10 pieces of adhere-wall culture unit altogether, wherein 5 pieces of superfine fibre towels, 5 pieces of flocking.Each adhere-wall culture unit is high 0.25 meter, wide 0.3 meter, adhere-wall culture unit interval 0.05 meter.Spirulina is inoculated in adherent culture device and cultivates cell surface.In culture medium receiver, culture fluid is that Zarrouk culture medium (consists of: every 1 liter of culture medium is containing sodium nitrate 2.5 grams, dipotassium hydrogen phosphate 0.5 gram, 1 gram of sodium chloride, bitter salt 0.2 gram, green vitriol 0.01 gram, potassium sulfate 1 gram, calcium chloride dihydrate 0.04 gram, ethylenediaminetetraacetic acid 0.08 gram, sodium bicarbonate 16.8 grams, boric acid 0.00286 gram, four 0.00186 gram of chloride hydrate manganese, Zinc vitriol 0.00022 gram, two 0.00039 gram of molybdic acid hydrate sodium, copper sulfate pentahydrate 0.0008 gram, cabaltous nitrate hexahydrate 0.00005 gram).Cultivating three days continuously, every day manually scrapes the cellular biomass cultivating cell surface, and washing and drying measures biomass yield.Experiment is a few days ago fine day, and outdoor sunlight light intensity at noon is about 1300 μm of olphotonm2/ s, at the 3rd day cloudy day, outdoor sunlight light intensity at noon is about 800 μm of olphotonm2/s.Result is as in figure 2 it is shown, during fine day, the biomass yield of the spirulina being seeded on superfine fibre towel can reach 60～70g/m2/ d, the cell product of Spirulina yield being seeded in flocking is about 40g/m2/d；The cell product of Spirulina yield being seeded on superfine fibre towel during the cloudy day is about 35g/m2/ d, the cell product of Spirulina yield being seeded in flocking is 12g/m2/d。
Comparative example 1:
Other conditions are identical with embodiment 1, it is 2012100515526 " a kind of Inserting-plate type microalgae semi-dry solid adherent culture device " that culture apparatus only changes into application number, utilizing the device in this patent to cultivate, the biomass yield of the spirulina being seeded on superfine fibre towel during fine day is 40～50g/m2/ d, the cell product of Spirulina yield being seeded in flocking is about 30～40g/m2/d；The cell product of Spirulina yield being seeded on superfine fibre towel during the cloudy day is about 30g/m2/ d, the cell product of Spirulina yield being seeded in flocking is 8g/m2/d。
Embodiment 2:
Testing location is Qingdao, and the testing time is in July, 2015.Support height is 0.3 meter, and culture tank height is 0.3 meter.Select superfine fibre towel as flexible material, to support the microalgae of adhere-wall culture cell surface.Culture apparatus arranges 12 pieces of adhere-wall culture unit altogether, each adhere-wall culture unit is high 0.25 meter, wide 0.3 meter, wherein 4 pieces of adhere-wall culture unit intervals 0.05 meter (light extension rate 10 ×), other 4 pieces of adhere-wall culture unit intervals are 0.1 meter (light extension rate 5 ×), and remaining 4 pieces of adhere-wall culture unit intervals is 0.2 meter (light extension rate 2.5 ×).Spirulina is inoculated in adherent culture device and cultivates cell surface.In culture medium receiver, culture fluid is that Zarrouk culture medium (consists of: every 1 liter of culture medium is containing sodium nitrate 2.5 grams, dipotassium hydrogen phosphate 0.5 gram, 1 gram of sodium chloride, bitter salt 0.2 gram, green vitriol 0.01 gram, potassium sulfate 1 gram, calcium chloride dihydrate 0.04 gram, ethylenediaminetetraacetic acid 0.08 gram, sodium bicarbonate 16.8 grams, boric acid 0.00286 gram, four 0.00186 gram of chloride hydrate manganese, Zinc vitriol 0.00022 gram, two 0.00039 gram of molybdic acid hydrate sodium, copper sulfate pentahydrate 0.0008 gram, cabaltous nitrate hexahydrate 0.00005 gram).Being fine day during experiment, outdoor sunlight light intensity at noon can reach 1500 μm of olphotonm2/s.Cultivating three days continuously, every day manually scrapes the cellular biomass cultivating cell surface, and washing and drying measures biomass yield.Result is as it is shown on figure 3, narrowing along with adhere-wall culture unit interval, the i.e. increase of light extension rate, and the biomass yield of spirulina is also gradually increased.When adhere-wall culture unit interval is 0.2 meter (extension rate is 2.5 ×), Biomass yield is about 30～40g/m2/d；When adhere-wall culture unit interval is 0.1 meter (light extension rate is 5 ×), cell product of Spirulina productivity is 35～50g/m2/d；When adhere-wall culture unit interval is 0.05 meter (light extension rate reaches 10 ×), the Biomass yield of spirulina can reach 45～60g/m2/d。
Embodiment 3:
Testing location is Qingdao, and the testing time is in July, 2015.Support height is 0.3 meter, and culture tank height is 0.3 meter.Select superfine fibre towel and flocking as flexible material, to support the microalgae of adhere-wall culture cell surface.Culture apparatus arranges 10 pieces of adhere-wall culture unit altogether, wherein 5 pieces of superfine fibre towels, 5 pieces of flocking.Each adhere-wall culture unit is high 0.25 meter, wide 0.3 meter, adhere-wall culture unit interval 0.05 meter.Huang Sizao (Tribonemasp.) is inoculated in adherent culture device and cultivates cell surface.In culture medium receiver, culture fluid is that BG11 culture medium (consists of: every 1 liter of culture medium is containing sodium nitrate 1.5 grams, dipotassium hydrogen phosphate 0.04 gram, bitter salt 0.0375 gram, two water drink 0.036 gram of calcium chloride, citric acid 0.006 gram, ferric ammonium citrate 0.006 gram, EDETATE DISODIUM 0.001 gram, sodium carbonate 0.02 gram, boric acid 0.00286 gram, four 0.00186 gram of chloride hydrate manganese, Zinc vitriol 0.00022 gram, two 0.00039 gram of molybdic acid hydrate sodium, copper sulfate pentahydrate 0.0008 gram, cabaltous nitrate hexahydrate 0.00005 gram).Cultivating three days continuously, every day manually scrapes the cellular biomass cultivating cell surface, and washing and drying measures biomass yield.Experiment is a few days ago fine day, and outdoor sunlight light intensity at noon is about 1300 μm of olphotonm2/ s, at the 3rd day cloudy day, outdoor sunlight light intensity at noon is about 800 μm of olphotonm2/s.As shown in Figure 4, during fine day, the biomass yield of the Huang Sizao being seeded on superfine fibre towel can reach about 70-80g/m2/ d, the yellow silk algae biomass yield being seeded in flocking is about 40～50g/m2/d；The yellow silk algae biomass yield being seeded on superfine fibre towel during the cloudy day can reach 42g/m2/ d, the yellow silk algae biomass yield being seeded in flocking is about 22g/m2/d。
Comparative example 2:
Other conditions are identical with embodiment 3, it is 2012100515526 " a kind of Inserting-plate type microalgae semi-dry solid adherent culture device " that culture apparatus only changes into application number, utilizing the device in this patent to cultivate, the biomass yield of the Huang Sizao being seeded on superfine fibre towel during fine day is 40～55g/m2/ d, the yellow silk algae biomass yield being seeded in flocking is about 30～40g/m2/d；The yellow silk algae biomass yield being seeded on superfine fibre towel during the cloudy day is about 30g/m2/ d, the yellow silk algae biomass yield being seeded in flocking is 10g/m2/d。
Other algae can also be cultivated by the present invention, and acquired results is similar, does not repeat them here.
The specific embodiment of the present invention is described in conjunction with accompanying drawing although above-mentioned; but the not restriction to invention protection domain; one of ordinary skill in the art should be understood that; on the basis of technical scheme, those skilled in the art need not pay various amendments or deformation that creative work can make still in protection scope of the present invention.
Claims (10)
1. the relative close type multi-thin-layer adherent culture device produced for microalgae, it is characterized in that: include bottom bracket, culture tank, liquid supplementation pipe and culture medium receiver, wherein, culture tank is fixed on the top of bottom bracket, described culture tank includes at least one adhere-wall culture unit, sidewall and base plate, each adhere-wall culture unit is substantially vertically arranged, leave, between adjacent two adhere-wall culture unit, the gap that sunlight passes through, culture tank is configured to the structure of only upper end open by sidewall and base plate, and culture tank leaves the position passing into carbon dioxide；
Each adhere-wall culture unit be provided above liquid supplementation pipe, each liquid supplementation pipe all connects with culture medium receiver；Being provided with the through hole for being flowed out by culture fluid on the base plate of culture tank, culture medium receiver is positioned over the lower section of this through hole, collects the culture fluid flowed down from this through hole.
2. culture apparatus according to claim 1, it is characterised in that: described through hole is positioned at the lowest point of culture medium base plate.
3. culture apparatus according to claim 1, it is characterised in that: leaving the aperture passing into carbon dioxide on the sidewall of described culture medium, this aperture is connected with carbon dioxide air source.
4. culture apparatus according to claim 1, it is characterised in that: the height of described culture tank is 0.3-1.0m.
5. culture apparatus according to claim 1, it is characterised in that: 0.01-10 times that distance is adherent cell height between adjacent two adhere-wall culture unit.
6. culture apparatus according to claim 1, it is characterised in that: the height of each adherent unit is lower than the height of culture tank.
7. culture apparatus according to claim 1, it is characterised in that: the position of each adherent unit is adjustable.
8. culture apparatus according to claim 1, it is characterised in that: described adhere-wall culture unit includes supporting axle and flexible material, supports axle and is arranged on the upper end of culture medium, and flexible material is suspended on support axle.
9. culture apparatus according to claim 1, it is characterised in that: described flexible material is fine hair material.
10. culture apparatus according to claim 1, it is characterised in that: described culture tank is square structure.
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| CN106754239A (en) * | 2016-11-16 | 2017-05-31 | 新奥科技发展有限公司 | A kind of microalgae solid culture system |
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| CN106754239B (en) * | 2016-11-16 | 2019-02-26 | 新奥科技发展有限公司 | A kind of microalgae solid culture system |
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