CN105749353A - 一种sis组织制备食管修复材料的方法 - Google Patents

一种sis组织制备食管修复材料的方法 Download PDF

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CN105749353A
CN105749353A CN201610094484.XA CN201610094484A CN105749353A CN 105749353 A CN105749353 A CN 105749353A CN 201610094484 A CN201610094484 A CN 201610094484A CN 105749353 A CN105749353 A CN 105749353A
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韩立军
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Suzhou Bai Bai Biotechnology Co., Ltd.
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Abstract

本发明公开了一种SIS组织制备食管修复材料的方法，首先取小肠粘膜制备SIS内层，将SIS裁剪成方形SIS单元，取一个SIS单元沿着圆柱体模具外壁的圆周铺设制成SIS内层，同时将羟基丁酸酯?羟基己酸酯共聚物与聚乳酸?聚乙醇酸共聚物混合后制备成混合溶液，然后在SIS内层的外表面上刷一层粘合剂，再将混合溶液均匀的涂抹在小肠粘膜下层的外表面上作为外层，得到食管修复材料；本发明采用小肠粘膜下层为内层，外层使用羟基丁酸酯?羟基己酸酯共聚物与聚乳酸?聚乙醇酸共聚物的混合物，食管修复材料生物力学强度和降解速率适宜，释放生长因子的能力与小肠粘膜下层一样，细胞相容性好，免疫原性低，溶血性低，可用于制备人工食管。

Description

一种 SIS 组织制备食管修复材料的方法
技术领域
本发明属于生物技术领域，特别涉及一种SIS组织制备食管修复材料的方法。
背景技术
小肠粘膜下层（Small Intestinal Submucosa，简称SIS）是一种可降解的天然生物学材料，其在组织修复与再生方面具有突出的生物学优势和广阔的应用前景。
SIS是以猪小肠粘膜下层为原料，原料来源广泛，通过生物衍生化处理技术，研制开发的新型的组织再生修复材料。SIS是当前广泛用于组织工程研究的天然细胞外基质类生物材料，具有良好的细胞相容性、含有多种生长因子、无免疫原性、具有抗微生物活性、各向异性、组织特异性诱导再生等特点，是较为理想的组织工程食管支架材料。
羟基丁酸酯-羟基己酸酯共聚物（PHBHHx）是微生物细胞内的一种高分子聚合物，具有良好的力学性能，其降解产物3-羟基丁酸是人体的常见代谢物，无毒副作用、易于塑形、但降解时间较长；聚乳酸-聚乙醇酸共聚物（polylacide-co-glycolide acid，PLGA）可通过改变聚乳酸与羟基乙酸的比例来调节降解时间，但材料力学强度不足。这类材料难以诱导组织再生，体内修复效果不好。
发明内容
发明目的：本发明提供一种SIS组织制备食管修复材料的方法，以解决现有技术中的问题。
技术方案：为实现上述目的，本发明采用的技术方案为：
一种SIS组织制备食管修复材料的方法，首先取小肠粘膜下层，经消毒、机械剥除、脱脂、脱细胞和去垢后制备SIS内层，将SIS裁剪成方形SIS单元，取一个SIS单元沿着圆柱体模具外壁的圆周铺设，刮除气泡后将此SIS单元互相覆盖的部分采用粘合剂粘合，再将一个新的SIS单元覆盖于上一个SIS单元，且低于上一个SIS单元1cm，依次向下设置若干个SIS单元制成SIS内层，同时将羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物混合后溶解在二氯甲烷中，制备成混合溶液，然后在SIS内层的外表面上刷一层粘合剂，再将混合溶液均匀的涂抹在小肠粘膜下层的外表面上作为外层，待凝固后冻干，取出圆柱体模具，得到食管修复材料。
进一步的，所述SIS单元的边长比圆柱体模具横截面的外圆周长长0.3mm。
进一步的，所述内层的每层SIS单元之间均通过粘合剂粘合。
进一步的，所述外层的复合量为0.5g /cm2，所述外层的羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物的摩尔比为1：1。
进一步的，所述羟基丁酸酯-羟基己酸酯共聚物中，羟基丁酸酯与羟基己酸酯的摩尔比为15:3。
进一步的，所述聚乳酸-聚乙醇酸共聚物中，聚乳酸与聚乙醇酸的摩尔比为1:1。
进一步的，所述粘合剂为浓度为10％的明胶溶液。
有益效果：本发明采用小肠粘膜下层为内层，外层使用羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物的混合物，食管修复材料生物力学强度和降解速率适宜，释放生长因子的能力与小肠粘膜下层一样，细胞相容性好，免疫原性低，溶血性低，可用于制备人工食管。
具体实施方式
下面结合实施例对本发明作更进一步的说明。
一种SIS组织制备食管修复材料的方法，首先取小肠粘膜下层，经消毒、机械剥除、脱脂、脱细胞和去垢后制备SIS内层，将SIS裁剪成方形SIS单元，取一个SIS单元沿着圆柱体模具外壁的圆周铺设，刮除气泡后将此SIS单元互相覆盖的部分采用粘合剂粘合，再将一个新的SIS单元覆盖于上一个SIS单元，且低于上一个SIS单元1cm，依次向下设置若干个SIS单元制成SIS内层，同时将羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物混合后溶解在二氯甲烷中，制备成混合溶液，然后在SIS内层的外表面上刷一层粘合剂，再将混合溶液均匀的涂抹在小肠粘膜下层的外表面上作为外层，待凝固后冻干，取出圆柱体模具，得到食管修复材料。
所述SIS单元的边长比圆柱体模具横截面的外圆周长长0.3mm。
所述内层的每层SIS单元之间均通过粘合剂粘合。
所述外层的复合量为0.5g /cm2，所述外层的羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物的摩尔比为1：1。
所述羟基丁酸酯-羟基己酸酯共聚物中，羟基丁酸酯与羟基己酸酯的摩尔比为15:3。
所述聚乳酸-聚乙醇酸共聚物中，聚乳酸与聚乙醇酸的摩尔比为1:1。
所述粘合剂为浓度为10％的明胶溶液。
以上所述仅是本发明的优选实施方式，应当指出：对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (7)

1.一种SIS组织制备食管修复材料的方法，其特征在于：首先取小肠粘膜下层，经消毒、机械剥除、脱脂、脱细胞和去垢后制备SIS内层，将SIS裁剪成方形SIS单元，取一个SIS单元沿着圆柱体模具外壁的圆周铺设，刮除气泡后将此SIS单元互相覆盖的部分采用粘合剂粘合，再将一个新的SIS单元覆盖于上一个SIS单元，且低于上一个SIS单元1cm，依次向下设置若干个SIS单元制成SIS内层，同时将羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物混合后溶解在二氯甲烷中，制备成混合溶液，然后在SIS内层的外表面上刷一层粘合剂，再将混合溶液均匀的涂抹在小肠粘膜下层的外表面上作为外层，待凝固后冻干，取出圆柱体模具，得到食管修复材料。
2.根据权利要求1所述的SIS组织制备食管修复材料的方法，其特征在于：所述SIS单元的边长比圆柱体模具横截面的外圆周长长0.3mm。
3.根据权利要求2所述的SIS组织制备食管修复材料的方法，其特征在于：所述内层的每层SIS单元之间均通过粘合剂粘合。
4.根据权利要求1所述的SIS组织制备食管修复材料的方法，其特征在于：所述外层的复合量为0.5g /cm2，所述外层的羟基丁酸酯-羟基己酸酯共聚物与聚乳酸-聚乙醇酸共聚物的摩尔比为1：1。
5.根据权利要求4所述的SIS组织制备食管修复材料的方法，其特征在于：所述羟基丁酸酯-羟基己酸酯共聚物中，羟基丁酸酯与羟基己酸酯的摩尔比为15:3。
6.根据权利要求4所述的SIS组织制备食管修复材料的方法，其特征在于：所述聚乳酸-聚乙醇酸共聚物中，聚乳酸与聚乙醇酸的摩尔比为1:1。
7.根据权利要求1所述的SIS组织制备食管修复材料的方法，其特征在于：所述粘合剂为浓度为10％的明胶溶液。
CN201610094484.XA 2016-02-22 2016-02-22 一种sis组织制备食管修复材料的方法 Pending CN105749353A (zh)

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
CN112891634A (zh) * 2021-02-05 2021-06-04 宁波市医疗中心李惠利医院 一种脱细胞小肠粘膜下层/聚乳酸-羟基乙酸共聚物复合支架及其制备方法和应用

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CN1324666A (zh) * 2001-06-22 2001-12-05 清华大学 一种生物材料和组织工程材料
CN1569253A (zh) * 2004-04-23 2005-01-26 清华大学 一种组织器官修复材料的制备方法
CN101433735A (zh) * 2007-11-13 2009-05-20 北京大清生物技术有限公司 一种sis组织修复材料的制备方法
CN102935247A (zh) * 2012-11-15 2013-02-20 四川大学华西医院 一种食管修复材料
CN103877619A (zh) * 2014-01-24 2014-06-25 北京大清生物技术有限公司 复合sis组织修复材料及其制备方法

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Publication number Priority date Publication date Assignee Title
CN1324666A (zh) * 2001-06-22 2001-12-05 清华大学 一种生物材料和组织工程材料
CN1569253A (zh) * 2004-04-23 2005-01-26 清华大学 一种组织器官修复材料的制备方法
CN101433735A (zh) * 2007-11-13 2009-05-20 北京大清生物技术有限公司 一种sis组织修复材料的制备方法
CN102935247A (zh) * 2012-11-15 2013-02-20 四川大学华西医院 一种食管修复材料
CN103877619A (zh) * 2014-01-24 2014-06-25 北京大清生物技术有限公司 复合sis组织修复材料及其制备方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112891634A (zh) * 2021-02-05 2021-06-04 宁波市医疗中心李惠利医院 一种脱细胞小肠粘膜下层/聚乳酸-羟基乙酸共聚物复合支架及其制备方法和应用

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