CN105748795A

CN105748795A - High-penetrability hoof protecting paste for dairy cows and preparation method of high-penetrability hoof protecting paste

Info

Publication number: CN105748795A
Application number: CN201610101283.8A
Authority: CN
Inventors: 邵素英
Original assignee: Yiwu Juhui Biotechnology Co Ltd
Current assignee: Yiwu Juhui Biotechnology Co Ltd
Priority date: 2016-02-24
Filing date: 2016-02-24
Publication date: 2016-07-13

Abstract

The invention discloses high-penetrability hoof protecting paste for dairy cows and a preparation method of the high-penetrability hoof protecting paste. A Chinese herbal medicinal extracting solution which effectively kills and inhibits infectious pathogens of dairy cow hoof disease, promotes the healing of a hoof wound, effectively prevents and treats multiple hoof diseases, and improves the autoimmunity and disease resistance of the dairy cows, and an aloe extract with functions of promoting medicine sink, effectively repairing cuticle and having high bonding property are taken as main raw materials, a plant extract which can change cuticle structures of dairy cow hoofs, promotes effective components of Chinese herbal medicine, aloe and the like to quickly penetrate into skin deep layer for absorbing and plant oil are scientifically compounded, a natural antioxidant which can protect effective ingredients of components from being oxidized and improve the physicochemical stability of the hoof protecting paste as well as keratinase and cutinase which promote the hoof protecting paste to be bonded and penetrate are added, and the substances are concentrated under reduced pressure to form paste, so that the hoof protecting paste which can comprehensively treat various types of hoof diseases of the dairy cows and has the characteristics of firm bonding, high penetrability and no antibiotics and chemical poisonous substances is finally prepared.

Description

Milch cow care of feed cream that a kind of permeability is strong and preparation method thereof

Technical field

The present invention relates to milch cow care of feed product, be specifically related to strong milch cow care of feed cream of a kind of permeability and preparation method thereof.

Background technology

In milk cattle cultivating, hoof disease, mastitis and Reproduction Disorder are cause milch cow to be forced to eliminate three big diseases, the limping of milch cow 90% causes due to hoof disease, therefore, hoof disease is the regular incidence of milch cow, and its cause of disease is mainly in daily ration calcium, phosphorus is uneven or trace element deficiency, cause cutin hoof to loosen, plus colony house bad environments, hoof often by fecaluria, mud dipping, hoof cutin softening and fall ill.It addition, the hard thing such as stone, iron filings, vegetation shred, fragments of glass, all can stab hoof soft tissue and cause infection inflammation.After milch cow suffers from hoof disease, appetite reduces, and quantity of motion reduces, and happiness is lain prone sleeping, limping, pain, occurs pain having hotness during palpation during percussion hoof.After expanding wound with poring iron, aperture at the bottom of hoof or namely have dirty black smelly water to flow out in hole, it can be seen that have ulcer between toe, cover cacodorous sphacelus.Severe one coronet is red and swollen, and pain substantially, can not stand, milk yield degradation, and reproductive capacity reduces, and finally eliminates by sick cattle.Along with developing rapidly of China milk industry, from abroad having introduced a large amount of high-quality milch cow, frozen semen and embryo, but these high yield cows are likely to less adapt to domestic feeding environment and diet nutrient, cause hoof disease sickness rate to have the trend of rising, cause huge economic loss to China's milk cow production.According to statistics, in China, some areas dairy cow farm hoof disease sickness rate is about 50%, and ill rear cow feeding amount reduces by 20%, milk yield reduces by 20%, eliminating ox head number because of hoof disease and account for the 50% of total superseded cattle, medical expense increases by 30%, and the economic loss caused because of hoof disease accounts for the 10% of annual profit.Therefore, preventing and treating Milk cow hoof disease, it is possible to reduce cow culling rate, significantly improve the economic benefit of China's milk cattle cultivating.

Hoof disease is a very ambiguous and general idea, is summed up it and actually includes a lot of disease, common are between ulcer at the bottom of hoof, hoof toe dermatitis, wart dermatitis etc. between skin proliferation, toe.Produce the main infectious cause of disease of the common cause of disease of above-mentioned hoof disease, metabolic disorder, environmental factors and hereditary factors, the measure correspondingly taked mainly carries out care of feed, foot paring and clinical treatment, be hoof disease prevent or adopt to treat in any way all can relate to a series of care of feed medicine, such as care of feed cream, care of feed liquid, hoof body lotion and care of feed spraying etc., in these medicines, antibiotic medicine occupies significant proportion, but use antibiotic product milch cow and the mankind can be produced significant impact in a large number, Drug resistance in milch cow and mankind's body is caused to strengthen, greatly compromise milch cow, the physical health of poultry feeders and epidemic prevention worker, breeding environment can be caused to pollute simultaneously, also can strengthen the safety risks of food (milk and beef).

Care of feed cream applies to a kind of care of feed product that milch cow is individual, after milch cow carries out individual epidemic prevention, foot paring treatment and clinical treatment, thoroughly clean Ungula Bovis seu Bubali portion, guarantee that hoof and interdigit do not have feces to remain, so as to totally, with Ungula Bovis seu Bubali brush, care of feed cream is applied to hoof and interdigit, if care of feed cream cohesive is better, can continuing 4-5 days, if good effect, care of feed cream is also reusable.When being in a bad way, it is possible to use care of feed binder, after maximum 4 days, it is necessary to dismantled by binder, then repeat to process with care of feed cream, it is not necessary to be wound around care of feed binder again.Therefore, care of feed cream is to prevent and treat the indispensable care of feed product of Milk cow hoof disease.

Chinese herbal medicine specimen is ruled together, and cures the symptoms, not the disease a lot than Western medicine, can effect a radical cure hoof disease, and side effect is little, pure natural, it is easy to degraded, environmentally friendly, and at present, relevant Chinese medicine care of feed product has been widely used for milk cattle cultivating.According to the breeding environment of milch cow, feeding pattern, the feature of bringing out of hoof disease and prevent and remedy measures, the therapeutic effect of bound drug, disease resistance, caking property and permeability are the key properties of care of feed cream.

Chinese patent CN104825486A discloses a kind of composition of medicine for treating claw ill, and including ethylenediamine dihydroiodide and copper sulfate, the mass mixing ratio of ethylenediamine dihydroiodide and copper sulfate is 1:2～2:3.Ethylenediamine dihydroiodide can react with copper sulfate, discharge free iodine, the atomic iodine of free state has superpower Oxidation, it is possible to makes protein denaturation and destroys the membrane structure of pathogen, thus reaching the purpose that prevention is infected and treated claw ill.Compositions Main Function in patent disclosed above is the surface oxidation sterilization utilizing the atomic iodine of strong oxidation to carry out disease hoof, only carry out disinfecting of surface, can not effect a radical cure, and intractable fungus effect is very micro-, osmosis is weak, and therapeutic effect is undesirable；Also with antibiotics use, to have side effects, and compositions be susceptible to chemical reaction simultaneously, not easily circulation, storage and use.

Chinese patent CN102100727A discloses a kind of Traditional Chinese Medicine for external application powder treating cattle footrot, the Chinese medicine of following weight portion process formulated: Olibanum 7-13 part, Os Draconis (calcined) 7-13 part, Lithargyrum's 7-13 part, catechu 9-15 part, Moschus 0.1-0.5 part, Borneolum Syntheticum 1-5 part, Borax 3-9 part.Powder in patent disclosed above, Effective Component of Chinese Medicine utilization rate is low, and loss is big, and external application is poor to sick hoof surface adhesiveness, it is easy to coming off, osmosis is poor, it is impossible to the focus below treatment top layer.

Chinese patent CN102552339A discloses a kind of nanoemulsion medicine for treating foot rot, this nano-emulsion particle diameter is between 1～100nm, being made up of the raw material of following component: pine tar 0.1%～10.0%, cinnamic aldehyde 0.1%～6.0%, carvacrol 0.1%～6.0%, Oleum Camphora 0.1%～5.0%, clobetasol propionate 0.01%～0.1%, surfactant 18.0%～35.0%, cosurfactant 0～7.0%, surplus are distilled water, the mass percent sum of mentioned component is 100%.This nano-emulsion has dissolving cutin, antipruritic, antiinflammatory, convergence, partly sterilised's anticorrosion and promotes the effects such as absorption, is mainly used in treatment animal foot rot.Invention increases the dissolubility of medicine, improve medicine stability and bioavailability, decrease drug dose, skin permeation rate is higher than the general formulations such as unguentum, and with low cost, has wide market prospect in field of veterinary.The medicament categories that newborn medicine disclosed above is composite is less, effect is single, it is impossible to the hoof disease of complete treatment milch cow, and containing glucocorticoids medicine clobetasol propionate and the bigger pine tar of toxicity, Camphora wet goods, easily penetrate into from site of injury, produce certain side effect.

Chinese patent CN102988654A discloses a kind of Chinese medicine preparation treating artiodactyl foot rot, and it is mixed and made into finished product after including the ground pulverizing of raw material of following parts by weight: dried Alumen 5～7 parts, Olibanum 1～3 part, Gypsum Fibrosum Preparatum 6～8 parts, Realgar 3～6 parts, Sanguis Draxonis 2～4 parts, salt essence 8～12 parts.Treatment artiodactyl foot rot, without any antibiotic and chemical synthetic drug, is had the significant effect of comparison, and cost is low by the Chinese medicine preparation of the present invention, economical and practical.Equally, preparation in patent disclosed above is powder, its Effective Component of Chinese Medicine utilization rate is low, and loss is big, and external application is poor to sick hoof surface adhesiveness, easily come off, osmosis is poor, it is impossible to the focus below treatment top layer, and containing toxic bigger Realgar, easily penetrate into from site of injury, produce certain side effect.

" pathogenic factor of Milk cow hoof disease and the comprehensive preventive health measures " that Xue Rongfa etc. deliver discloses the herbal treatment of a kind of Milk cow hoof disease: conventional Pulvis Indigo Naturalis is treated, prescription is Indigo Naturalis 60g, Borneolum Syntheticum 30g, iodoform 30g, Calomelas 15g, Os Draconis 6g, mixed grinding becomes smalls, cleans and removes slough, after disinfecting, by Pulvis Indigo Naturalis plug in wound, wrapping hoof (herding and forage science .2015,36 (4): 118-119).Herbal treatment disclosed above adopts powder equally, and Effective Component of Chinese Medicine utilization rate is low, and loss is big, also binder free composition in component, external application is poor to sick hoof surface adhesiveness, it is easy to coming off, osmosis is poor, can not treating the focus on below top layer, Calomelas therein is severe toxicity precursor, and safety is poor.

To sum up, prepare a kind of can complete treatment milch cow all kinds hoof disease, be firmly bonded, permeability is strong, care of feed cream safe and efficient, without any antibiotic and chemical toxic substances be numerous raisers in the urgent need to, be also responsibility and the pursuit of those skilled in the art.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing milch cow care of feed cream; effectively to kill and to suppress Milk cow hoof disease infectivity pathogen and to promote hoof wound healing, it is possible to effectively prevent and treat multiple hoof disease, improve the extracts of Chinese herbal medicine of milch cow autoimmunity and premunition and have promotion drug osmotic, effectively repair horny layer, the Aloe extract of strong cohesive property is primary raw material；Science compound highly effective bonding modified dietary fiber and water-soluble chitosan, milch cow hoof horny layer structure the plant extract promoting the effective ingredient rapid osmotic such as Chinese herbal medicine to deep skin to absorb and vegetable oil can be changed；With the addition of simultaneously and component effective ingredient can be protected from oxidation; improve the Natural antioxidant of care of feed cream physical and chemical stability; and with the addition of keratinase, the at etc. that promote care of feed cream to bond and permeate; final prepare a kind of can complete treatment milch cow all kinds hoof disease, be firmly bonded, permeability is strong, safe and efficient, care of feed cream without any antibiotic and chemical toxic substances; reduce milk cattle cultivating cost; improve fanning economics; add the safety of milch cow deep-processed food, protect environment.

In order to achieve the above object, the present invention adopts the following technical scheme that

The milch cow care of feed cream that a kind of permeability is strong, is mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 30-50 part, Aloe extract 10-30 part, modified dietary fiber 10-15 part, water-soluble chitosan 2-8 part, plant extract 8-12 part, vegetable oil 1-5 part, Natural antioxidant 1-4 part, keratinase 0.5-1.5 part, at 0.1-1 part；

Preferably, the milch cow care of feed cream that a kind of permeability is strong, mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 35-45 part, Aloe extract 15-25 part, modified dietary fiber 12-14 part, water-soluble chitosan 4-6 part, plant extract 9-11 part, vegetable oil 2-4 part, Natural antioxidant 2-3 part, keratinase 0.8-1.2 part, at 0.3-0.8 part；

It is highly preferred that the milch cow care of feed cream that a kind of permeability is strong, mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 40 parts, Aloe extract 20 parts, modified dietary fiber 13 parts, water-soluble chitosan 5 parts, plant extract 10 parts, vegetable oil 3 parts, the Natural antioxidant 2.5 parts, keratinase 1.0 parts, at 0.5 part；

Further, described extracts of Chinese herbal medicine is effectively to kill and suppress Milk cow hoof disease infectivity pathogen, and there is promoting blood circulation and stopping pain, detumescence and promoting granulation, dampness hemostasis, parasite killing removing toxic substances, removing dampness, sore, spasmolytic, heat of dispelling, it is possible to the Chinese medicine effectively preventing and treating multiple hoof disease, raising milch cow autoimmunity and premunition is prepared by raw material；

Preferably, the preparation method of described extracts of Chinese herbal medicine, comprise the steps: to count by weight, weigh Olibanum 20-25 part, Sanguis Draxonis 18-20 part, Radix Astragali 13-16 part, Myrrha 12-15 part, Os Draconis 12-15 part, Radix Achyranthis Bidentatae 8-12 part, Cortex Acanthopancis 7-9 part, Pulvis Fumi Carbonisatus 4-6 part, Herba Portulacae 4-6 part, Radix Notoginseng 2-4 part, Cortex Dictamni 1-3 part, Cortex Meliae 1-3 part；Put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 with breast acid for adjusting pH value, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 with breast acid for adjusting pH value, enzymolysis 2-4h, finally add the mixture of 0.5-3 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:1-3, control temperature and keep 3-4h to 60-78 DEG C, filter, obtain the first filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, be then cooled to 25-35 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

Described compound enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 5-7:2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described Aloe extract is with Aloe for primary raw material, the waste residue such as peel or Fructus Crataegi, Radix Betae, Fructus Mali pumilae such as Fructus Citri grandis that the composite pectin content of science is higher, Fructus Citri Limoniae, orange, mandarin orange, Fructus Vitis viniferae extracts through ultrasonic cleaning, high-voltage pulse, hot water extraction and prepared；

Preferably, the preparation method of described Aloe extract, comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, being cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Adding the deionized water of ground product quality 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, carries out high voltage pulse electric field processing when pulse frequency 200-400Hz, then normal pressure boils 30-50min, Bag filter, filtering residue boils 40-60min, Bag filter, merging filtrate with its filtrate normal pressure of quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

Further; described modified dietary fiber is the impact with strong retentiveness, dilatancy, thickening property, adsorptivity and acid and alkali, alkali, salt that dietary fiber is processed through physics, chemical or biological method and obtained, and significantly improves caking property and the physical and chemical stability of milch cow care of feed cream；

Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber；

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min；Enzymolysis solution filters, and namely filtrate reduced in volume, vacuum lyophilization, micronizing obtain modified dietary fiber to particle diameter 0.1-0.3mm；

Described enzyme is cellulase, glucanase, xylanase, pectase 5-7:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.

Further, described plant extract is can change milch cow hoof horny layer structure, to promote that care of feed cream component rapid osmotic is prepared for raw material to deep skin the natural plants that absorbs.Such as: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae etc.；

nullPreferably，The preparation method of described plant extract，Comprise the steps: Radix Angelicae Sinensis、Folium clausenae lansii、Flos Caryophylli、Radix Salviae Miltiorrhizae、Herba Menthae is respectively washed、Drain，10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 4.5-5.5 of mixed material quality 0.3-0.5 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-1mm,It is subsequently added into the water of ground product quality 4-6 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 8-10% obtains plant extract；

Described mixed enzyme is cellulase, hemicellulase, pectase, tannase, protease 3-5:3-5:2-4:1-3:0.3-0.5 Homogeneous phase mixing in mass ratio.

Further, the described Natural antioxidant contains any one or a few in tea polyphenols, grape pip procyanidin, Herba Rosmarini Officinalis extract, Radix Glycyrrhizae extract, Folium Pruni extract and Vc；

Preferably, the quality group of the described Natural antioxidant becomes: tea polyphenols: grape pip procyanidin: Vc=5-7:2-4:1-3.

The preparation method that another object of the present invention is to provide the strong milch cow care of feed cream of above-mentioned permeability, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100-200 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 200-400W, ultrasonic Treatment 18-28min is carried out when frequency 35-45KHz, finally by high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 80-100MPa, making homogenizer outlet material particle diameter is 150-450nm, obtain nanoparticle mixed liquor；Seal; room temperature natural fermentation 3-5d; then heat to 60-70 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 20-30min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

The using method of the milch cow care of feed cream that permeability of the present invention is strong: first clean Ungula Bovis seu Bubali; find focus, determine whether operative treatment according to the type of hoof disease and the order of severity: 1) if slight, after would carrying out simple foot paring; then with suds and 0.1% bromogeramine solution cleaning and sterilizing; dry hoof, row routine disinfection, with Ungula Bovis seu Bubali brush, care of feed cream is applied to wound and around; in clean, dry colony house; strengthen observing and nursing, it is not necessary to change dressings, within general 2-3 days, can fully recover.2) if serious, operative treatment is carried out, for wart dermatitis: first repair Ungula Bovis seu Bubali, to vegetation surrounding skin cropping, shaving；Then with suds and 0.1% bromogeramine solution cleaning and sterilizing, hoof, row routine disinfection are dried.Pricking tourniquet at palm middle part, left hand is held mosquito forceps and is fixed by vegetation, and the right hand is held scalpel and done fusiform incision on the healthy skin of its base portion, the degree of depth, to cut through skin holostrome, exposes fat, hemostasis by compression between toe, arrange fresh wound surface, repeatedly rinse posterior tubercle skin suture with normal saline well.Then with Ungula Bovis seu Bubali brush care of feed cream is applied to wound and around, then applies with gauze pad and bandage after wound, remove tourniquet.Postoperative milch cow is placed in clean, dry colony house, strengthens observing and nursing, within about 3 days, untie binder, change dressings, it is prevented that secondary infection, within general 4-6 days, can fully recover.

Conventional epidemic prevention, the epidemic prevention of normal foot paring, hoof disease type all can refer to the prevention of above-mentioned using method or treatment.

Beneficial effect:

nullThe present invention is effectively to kill and to suppress Milk cow hoof disease infectivity pathogen and to promote hoof wound healing，Can effectively prevent and to treat multiple hoof disease、Improve the extracts of Chinese herbal medicine of milch cow autoimmunity and premunition and there is promotion drug osmotic、Effectively repair horny layer、The Aloe extract of strong cohesive property is primary raw material，Interpolation can change milch cow hoof horny layer structure and promote Chinese herbal medicine、Plant extract that the effective ingredient rapid osmotic such as Aloe to deep skin absorbs and vegetable oil，By ultrasonic disperse、Dissolving technology organically combines with high pressure homogenization technique，Further by material effective component granule nanorize，Prepare into more stable、It is easier to infiltration and the nanoparticle mixed solution absorbed，Through airtight、After room temperature natural fermentation, its effective ingredient granule is softer、Fine and smooth，Synergistic effect is higher、Permeability、Absorbability and caking property are more preferably，Not only science compound highly effective bonding modified dietary fiber and water-soluble chitosan，And with the addition of and component effective ingredient can be protected from oxidation，Improve the Natural antioxidant of care of feed cream physical and chemical stability，With the addition of the keratinase promoting that care of feed cream bonds and permeates simultaneously、At，It is paste through concentrating under reduced pressure，Final prepared one can complete treatment milch cow all kinds hoof disease、It is firmly bonded、Permeability is strong、Safe and efficient、Care of feed cream without any antibiotic and chemical toxic substances，It is with natural plants for raw material，Adopt low temperature processing techniques，Without any chemical toxic substances，Prevention and treatment Milk cow hoof disease process do not use any antibiotic，Reduce milk cattle cultivating cost，Improve fanning economics，Add the safety of milch cow deep-processed food，Protect environment.Tests prove that, various Milk cow hoof disease can quickly, effectively be treated by care of feed cream of the present invention, claw ill is had the effect of antibacterial infection, removing toxic substances convergence, removing the necrotic tissue and promoting granulation and promotion wound healing, concrete technique effect such as test example 7: permeability is strong, drug effect is high compared with commercial care of feed cream, caking property is strong.When medication 5 days, its cure rate reached 65.7-80%, total effective rate reaches 88.6-96%；Respectively than the high 49.7-66.7% of matched group (13.3-16%) and (55-80%) and 8.6-41%；When medication 10 days, its cure rate reached 90-96%, total effective rate reaches 96.7-100%；Respectively than the high 14-36% of matched group (60-76%) and (75-88%) and 8.7-25%；Reach same therapeutic efficiency, treatment time at least shortens 5 days, particularly to wart dermatitis, foot rot and other type of hoof disease therapeutic effect are best, illustrate simultaneously, therapeutic process is not when using antibiotics auxiliary treatment, care of feed cream treatment time of the present invention is short, efficiency is high, not only reduce milk cattle cultivating cost, add fanning economics, and improve the foodsafety of milch cow deep processed product, also protect breeding environment simultaneously, stop because using antibiotic, the potential safety hazard that chemistry and noxious substance produce, a new green shortcut is opened for application care of feed cream treatment Milk cow hoof disease.Tests prove that, milch cow after treatment is remarkably improved the various disease ability of the food-intake of milch cow, immunity, production performance, reproductive performance and opposing, it is specifically shown in embodiment 8:1) test group care of feed cream trend that milch cow Body Condition Score is significantly improved, milch cow can be improved to the digestion of nutrient substance, absorption and metabolism, may advantageously facilitate and improve the synthesis of the framework material such as vivo protein, fat, hence it is evident that the body condition of milch cow can be improved.2) test group is compared with matched group, and head day increases milk yield 7.51 kilograms, improves 19.57%, and t checks significant difference (P < 0.05), and care of feed cream is remarkably improved the milk yield of lactating cow.3) Immunoglobulin IgA in cow serum, IgM, IgG content be the important indicator judging milch cow humoral immunity level.As seen from the experiment, cow birth stress cause that serum immune globulin IgA, IgM, IgG content sharply decline, and humoral immune function is suppressed.Test group care of feed cream can increase before cow birth after 1 week, the childbirth same day and childbirth the content of 1 week serum IgA, IgM, IgG, enhancing humoral immune function.4) compared with matched group, test group Diseases of Cow can be greatly shortened nearly 13 days of the time of milch cow postpartum heat, improve the conception rate 19.6% of the first feelings phase milch cow in puerperal, increase the ratio of oestrusing 17.7% of Postpartum Cows colony, be effectively improved the reproductive performance of milch cow.5) result above shows: test group is compared with matched group, in prevention postpartum disease of cow, the sickness rate of various disease can be significantly reduced, on average reduce 11.87-28.1%, t checks significant difference (P < 0.05), care of feed cream can significantly reduce the incidence rate of the diseases such as milch cow mastitis in puerperal, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and placenta retention, improves the health status of milch cow.

Concrete know-why is as follows:

null1. the extracts of Chinese herbal medicine that prepared by the present invention is effectively to kill and suppress Milk cow hoof disease infectivity pathogen，And there is promoting blood circulation and stopping pain、Detumescence and promoting granulation、Dampness is stopped blooding、Parasite killing detoxifies、Removing dampness、Sore、Spasmolytic、Dispel heat，Can effectively prevent and treat multiple hoof disease、The Chinese medicine improving milch cow autoimmunity and premunition is that raw material is through ultrasonic cleaning、Ultrasonic assistant microwave extraction、Low temperature enzymolysis and water extracting alcohol carry and prepare，Extracting solution active constituent content is high，Medical material utilization rate is high、Extraction time is short、Extraction ratio is high、Extraction cost is low，Drug effect highly effective and safe，Can effectively prevent and prevent various Milk cow hoof disease，What be different from prior art is，While reaching better prevention and therapeutic effect，Science of the present invention is composite various conventional、Effective and that the property of medicine is soft natural Chinese medicinal herb，Without Calomelas、Realgar、The severe toxicity Chinese medicine such as Plumbum preparatium，Also without using any chemical drugs and antibiotic，Prevent it from entering blood circulation by wound，Further increase milk、The foodsafety of the deep processed products such as beef，Improve the safety of the medicine use of poultry feeders and the safety of breeding environment simultaneously，Protect environment.Above-mentioned drug effect is the synergistic result of each herbal component, not the superposition of single component drug effect.

null2. the Aloe extract of the preparation of the present invention is with Aloe for primary raw material，The composite Citrus pericarp of science、Pericarpium Vitis viniferae、Fructus Crataegi marc is through ultrasonic cleaning、High-voltage pulse extracts、Hot water extraction and prepared，Remain Aloe gel therein to greatest extent、Pectin、Aloe-emodin、Aloin A、Wound hormone and polysaccharide peptide manna、The multiple bioactive ingredients such as Aloe polysaccharide，Its viscosity is considerably beyond the viscosity of single Aloe gel and pectin，Substantially increase the caking property of care of feed cream，The more important thing is that the multiple bioactive ingredients in Aloe can promote that wound healing and restoration ecosystem do not stay scar，Powerful reparation horny layer，Viral infection resisting、Pesticidal and sterilizing、Sun-proof anticancer、Defying age removing toxic substances，There is multiple efficacies，May additionally facilitate and carry other effective ingredient and penetrate into hoof deep layer，The osmotic efficiency of raising itself and Chinese herbal medicine，Shorten time of penetration，Work in coordination with other compositions，Such as: modified dietary fiber、Plant extract、Chitosan etc.，Its drug effect and serviceability are higher，It is especially suitable for the drug effect of milch cow care of feed cream、Safety and administering mode and method requirement.

3. the plant extract that prepared by the present invention is can change milch cow hoof horny layer structure, to promote that the effective ingredient rapid osmotic such as Chinese herbal medicine to deep skin and absorb, with effect a radical cure focus natural plants for raw material, the extract at low temperature techniques such as science is composite and cleaned, moistening, freezing and pulverizing, high-pressure pulse electric extraction, ultrasonic assistant microwave extraction, biological enzymolysis and prepare, such as: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae etc.；Its active constituent content is high; employing natural plant extracts is penetration enhancer; instead of the chemistry penetration enhancer such as the azone of prior art, dimethyl sulfone, tween, propylene glycol, ethanol completely and short saturating effect is more significantly; not only increase the permeability of care of feed cream; and improve the pharmacological property of care of feed cream; add the safety in utilization of care of feed cream simultaneously, protect environment.Working in coordination with extracts of Chinese herbal medicine, its drug effect, safety and usability are higher.

4. the impact with strong retentiveness, dilatancy, thickening property, adsorptivity and acid and alkali, alkali, salt that dietary fiber is processed through physics, chemical or biological method and obtains by the modified dietary fiber that prepared by the present invention, significantly improves caking property and the physical and chemical stability of milch cow care of feed cream；High adsorption capacity, after modified, cellulosic specific surface area increases, network is enriched, absorption affinity strengthens, and further enhancing the caking property of care of feed cream, and powerful embedding effect can prevent breeding environment (oxygen, temperature, illumination, the water activity etc.) factor impact on product drug effect, stabilize the property of medicine of care of feed cream, extend shelf-life of care of feed cream and prevent and useful life in therapeutic process, use the property of medicine.Working in coordination with chitosan, effect is more preferably.

5. science of the present invention is composite a small amount of keratinase and at, can decompose milch cow hoof horny layer appropriateness, increases effective ingredient rapid osmotic to internal layer, shortens treatment time and increases therapeutic effect；The horny layer that appropriateness is decomposed simultaneously is more prone to and Aloe gel, pectin, chitosan, modified dietary fiber bonding, fastness when further enhancing the caking property of care of feed cream and use, difficult drop-off, particularly hoof disease epidemic prevention and do not use care of feed binder treatment hoof disease time even more important.

6. the Natural antioxidant that science of the present invention is composite, can effectively prevent keratinase, at, plant wet goods effective ingredient from aoxidizing, lose effect, indirectly enhance the stability of care of feed cream, drug effect, permeability and caking property, finally improve safety and the usability of care of feed cream.

7. to overcome traditional and existing care of feed cream high temperature fried for preparation method of the present invention, process time is long, efficiency is low, drug effect is low, the defects such as energy consumption is high, omnidistance employing low temperature processing techniques, effectively prevent the loss of material effective component, by ultrasonic dissolution, dispersion, high pressure homogenize, low temperature fermentation, the technology such as concentrating under reduced pressure organically combine, further enhancing the synergistic effect of material effective component and the caking property of care of feed cream, permeability and absorbability, shorten the process time, improve work efficiency, reduce production cost, improve product quality, improve fanning economics, add the safety of milch cow deep-processed food, protect environment.

It should be noted that the medicinal effects of care of feed cream of the present invention is the result that each component is mutually worked in coordination with, interacted, the not superposition of simple raw material function, the science of each raw material components is composite and extracts, the effect produced, considerably beyond the superposition of each single component function and effect, has advanced preferably and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of extracts of Chinese herbal medicine:

The preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, weighs Olibanum 23 parts, Sanguis Draxonis 19 parts, the Radix Astragali 15 parts, Myrrha 14 parts, Os Draconis 14 parts, Radix Achyranthis Bidentatae 10 parts, Cortex Acanthopancis 8 parts, Pulvis Fumi Carbonisatus 5 parts, Herba Portulacae 5 parts, Radix Notoginseng 3 parts, Cortex Dictamni 2 parts, Cortex Meliae 2 parts；Put in the ultrasonic washing unit filling 0.2% sodium bicarbonate solution and clean 12min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixed material, control temperature 80 DEG C and keep 3h, then it is cooled to 45 DEG C, is 4.0 with breast acid for adjusting pH value, carries out microwave extraction 12min when power 200W, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 1.0% carries out enzymolysis, is 6.2 with breast acid for adjusting pH value, enzymolysis 3h, finally adding the mixture of 2 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:2, controls temperature and keeps 3.5h to 70 DEG C, filter, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control temperature 90 DEG C and keep 2h, be then cooled to 30 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 3:2, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

Described compound enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 6:3:3:2:2 Homogeneous phase mixing in mass ratio.

2. the preparation of Aloe extract:

The preparation method of described Aloe extract, comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 8min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 23:4:3:2 Homogeneous phase mixing, then in-23 DEG C of freezing 20min；Pulverizing immediately, particle diameter is 0.5mm；Adding the deionized water of ground product quality 4 times, mix homogeneously, is 2.5 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 40kV/cm under room temperature, burst length 500 μ s, carries out high voltage pulse electric field processing when pulse frequency 300Hz, then normal pressure boils 40min, Bag filter, the filtering residue filtrate normal pressure of its quality 2 times boils 50min, Bag filter, merging filtrate, it is 8% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

3. the preparation of modified dietary fiber:

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 7:4:3:2 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 200W, 35KHz condition supersound extraction 12min, then at electric field intensity 30kV/cm, burst length 400 μ s, carries out high voltage pulse electric field processing 12min when pulse frequency 300Hz；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.5%, in 50 DEG C of enzymolysis 35min；Enzymolysis solution filters, and namely filtrate reduced in volume, vacuum lyophilization, micronizing obtain modified dietary fiber to particle diameter 0.2mm；

Described enzyme is cellulase, glucanase, xylanase, pectase 6:3:2:1.5 Homogeneous phase mixing in mass ratio.

4. the preparation of plant extract:

The preparation method of described plant extract, comprise the steps: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae is respectively washed, drain, 11:8:8:5:2 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 5h that pH value is 5.0 of mixed material quality 0.4 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, ground product particle diameter 0.8mm, it is subsequently added into the water of ground product quality 5 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, high voltage pulse electric field processing 25min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 18min when power 200W in room temperature, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 1.0%, in 50 DEG C of enzymolysis 40min；Enzymolysis solution filters, filtrate reduced in volume to solid content is 9% namely obtain plant extract；

Described mixed enzyme is cellulase, hemicellulase, pectase, tannase, protease 4:4:3:2:0.4 Homogeneous phase mixing in mass ratio.

Extracts of Chinese herbal medicine that example 2 below-6 uses, Aloe extract, modified dietary fiber, plant extract are embodiment 1 and prepare, other is commercial commodity, and wherein water-soluble chitosan, the Natural antioxidant, keratinase, at are powdery.

Embodiment 2

The quality group of the described Natural antioxidant becomes: tea polyphenols: grape pip procyanidin: Vc=6:3:2；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 150 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 300W, carries out ultrasonic Treatment 23min when frequency 40KHz, finally by high pressure homogenizer in temperature 65 DEG C, circulate 3 times when pressure 90MPa so that homogenizer outlet material particle diameter is 300nm, obtains nanoparticle mixed liquor；Seal; room temperature natural fermentation 4d; then heat to 65 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 25min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

Embodiment 3

Extracts of Chinese herbal medicine 30 parts, Aloe extract 10 parts, modified dietary fiber 10 parts, water-soluble chitosan 2 parts, plant extract 8 parts, vegetable oil 1 part, the Natural antioxidant 1 part, keratinase 0.5 part, at 0.1 part；

The quality group of the described Natural antioxidant becomes: tea polyphenols: grape pip procyanidin: Vc=5:2:1；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 200W, carries out ultrasonic Treatment 18min when frequency 35KHz, finally by high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 80MPa so that homogenizer outlet material particle diameter is 450nm, obtains nanoparticle mixed liquor；Seal; room temperature natural fermentation 3d; then heat to 60 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 20min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

Embodiment 4

Extracts of Chinese herbal medicine 50 parts, Aloe extract 30 parts, modified dietary fiber 15 parts, water-soluble chitosan 8 parts, plant extract 12 parts, vegetable oil 5 parts, the Natural antioxidant 4 parts, keratinase 1.5 parts, at 1 part；

The quality group of the described Natural antioxidant becomes: tea polyphenols: grape pip procyanidin: Vc=7:4:3；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 200 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 400W, carries out ultrasonic Treatment 28min when frequency 45KHz, finally by high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 100MPa so that homogenizer outlet material particle diameter is 150nm, obtains nanoparticle mixed liquor；Seal; room temperature natural fermentation 5d; then heat to 70 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 30min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

Embodiment 5

Extracts of Chinese herbal medicine 35 parts, Aloe extract 15 parts, modified dietary fiber 12 parts, water-soluble chitosan 4 parts, plant extract 9 parts, vegetable oil 2 parts, tea polyphenols 2 parts, keratinase 0.8 part, at 0.3 part；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 400W, carries out ultrasonic Treatment 28min when frequency 35KHz, finally by high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 100MPa so that homogenizer outlet material particle diameter is 450nm, obtains nanoparticle mixed liquor；Seal; room temperature natural fermentation 3d; then heat to 70 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 20min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

Embodiment 6

Extracts of Chinese herbal medicine 45 parts, Aloe extract 25 parts, modified dietary fiber 14 parts, water-soluble chitosan 6 parts, plant extract 11 parts, vegetable oil 4 parts, Herba Rosmarini Officinalis extract 3 parts, keratinase 1.2 parts, at 0.8 part；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 200 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 200W, carries out ultrasonic Treatment 18min when frequency 45KHz, finally by high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 80MPa so that homogenizer outlet material particle diameter is 150nm, obtains nanoparticle mixed liquor；Seal; room temperature natural fermentation 5d; then heat to 60 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 30min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

The embodiment 7 care of feed cream of the present invention effect test when treating Milk cow hoof disease

For verifying the clinical efficacy of care of feed cream of the present invention, three dairy cow farms in Hebei province and Inner Mongolia Autonomous Region in October ,-2015 in June, 2014, select 320 example Milk cow hoof diseases altogether, wherein wart dermatitis 100 example, hypertrophy 60 example between toe, ulcer 70 example at the bottom of hoof, foot rot 50 example, other type hoof disease 40 example, it is randomly divided into two groups: test group and matched group according to hoof disease type, care of feed cream prepared by the embodiment of the present invention 2 and commercial care of feed cream is not used to treat through plant veterinary and foot paring work point, test group and matched group are except medication (care of feed cream) difference, other is raised and way to manage, daily ration, Therapeutic Method, feed environment, efficacy evaluations etc. are all identical.

Case selection standard: having symptom of substantially walking lamely, happiness is lain prone sleeping, and dysstasia or stand up time are shorter；The suffering limb unreal or each limb hoof of heavy burden alternately bears a heavy burden；Palpation suffering limb hoof temperature rise, pain sensitivity during percussion.Sick cattle performance spirit is depressed, and inappetence, milk yield declines, and body temperature raises sometimes, up to more than 40 DEG C.Such as aggravation, indivedual ill milch cows show some General Symptomies, and severe one affected part is red and swollen, does not rise sleepingly.

Curative effect determinate standard: observe sick cattle symptom, determine curative effect according to criterion for the 5th day, 10 days after medication.Suffering from cattle limping to disappear, pain disappears, and swelling is disappeared, and wound healing is for curing；Pain substantially alleviates, swelling range shorter, walk lamely alleviate or inconspicuous be effective；Pain does not disappear, and swelling, without disappearing, is walked lamely without disappearing, had dyskinetic for invalid.Total effectively head number is equal to recovery from illness and effective head number summation.

The result for the treatment of is carried out statistics and analysis, specifically as shown in table 1,2:

Table 1: 5 days therapeutic outcomes of medication

Table 2: 10 days therapeutic outcomes of medication

Result above shows: various Milk cow hoof disease can quickly, effectively be treated by care of feed cream of the present invention, claw ill has the effect of antibacterial infection, removing toxic substances convergence, removing the necrotic tissue and promoting granulation and promotion wound healing, and permeability is strong, drug effect is high compared with commercial care of feed cream, caking property is strong.When medication 5 days, its cure rate reached 65.7-80%, total effective rate reaches 88.6-96%；Respectively than the high 49.7-66.7% of matched group (13.3-16%) and (55-80%) and 8.6-41%；When medication 10 days, its cure rate reached 90-96%, total effective rate reaches 96.7-100%；Respectively than the high 14-36% of matched group (60-76%) and (75-88%) and 8.7-25%；Reach same therapeutic efficiency, treatment time at least shortens 5 days, particularly to wart dermatitis, foot rot and other type of hoof disease therapeutic effect are best, illustrate simultaneously, therapeutic process is not when using antibiotics auxiliary treatment, care of feed cream treatment time of the present invention is short, efficiency is high, not only reduce milk cattle cultivating cost, add fanning economics, and improve the foodsafety of milch cow deep processed product, also protect breeding environment simultaneously, stop because using antibiotic, the potential safety hazard that chemistry and noxious substance produce, a new green shortcut is opened for application care of feed cream treatment Milk cow hoof disease.

It should be understood that care of feed cream prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

The impact on milk cow production performance, reproductive performance and anti-disease of the embodiment 8 care of feed of the present invention cream

1 materials and methods

1.1, test material

1.1.1, product of the present invention

1.1.2, test milch cow: embodiment 7 experimental group and matched group are through treating the milch cow of hoof disease.

1.2, test daily ration composition: test group and matched group adopt same feeding and management method.Daily ration consists of concentrate (Semen Maydis 52.7%, wheat bran 8%, bean cake 7%, cottonseed meal 9%, Semen arachidis hypogaeae dregs 9%, corn DDGS 8%, premix material 7115A6%), and coarse fodder is corn silage, Leymus chinensis (Trin.) Tzvel., the bean stalks of rice, wheat, etc., Herba Medicaginis etc..

1.3, test method: according to milch cow breeding record, test group is except taking same method to feed with matched group, all for the concentrate 4-8kg of giving milk of autogamy in examination Cow-feeding field, it is adjusted according to raising stage and milk yield, corn silage quantitative feeding 20kg/ days, roughage (Leymus chinensis (Trin.) Tzvel., the bean stalks of rice, wheat, etc., Herba Medicaginis) free choice feeding.

1.4, production, metabolism, reproductive performance record: in during 120 days puerperal, measure and record the milk yield of every cow head；Observe, evaluate milch cow body condition；Detection reproductive performance；During 120 days puerperal, observed and recorded is participated in the experiment the rutting situation of milch cow, and whether gestation is as the criterion with examination per rectum in latter 60 days of breeding.

1.5, disease surveillance and record: to Dairy Cows diseases such as () displacement of mastitis, endometritis, ketoacidosis, cud, intractable apositia, lochiopyra and placenta retention detection, the same day was monitored placenta retention incidence in childbirth, and definition afterbirth not discharge person in 12-24 hour is placenta retention.Adopt Ketone benzene detection Ketosis, adopt CMT-Test reagent detection mastitis.

1.6, initial data is processed by statistical analysis EXCEL software, then carries out variance analysis by SAS [11] method.

2. result of the test

The impact on milch cow body condition of the 2.1 care of feed cream:

Table 3 milch cow 5 part Body Condition Score standard

Score value	Vertebra portion	Flank	Buttocks both sides	Root of the tail both sides	Hipbone, ischium joint
						1	Very prominent	Root root is visible	Excessive settlement	Crypts is very deep	Very prominent
2	Substantially prominent	Most visible	Substantially it sink	Crypts is obvious	Substantially prominent
						3	Somewhat prominent	Minority is visible	Somewhat sagging	Crypts is slightly aobvious	Somewhat prominent
4	Straight	Lose completely	Straight	Crypts does not show	Do not show prominent
						5	Plentiful	Plentiful	Plentiful	Plentiful	Plentiful

Table 4 milch cow body condition visual examination appraisal result

Result above shows: the trend that milch cow Body Condition Score is significantly improved by test group care of feed cream, milch cow can be improved to the digestion of nutrient substance, absorption and metabolism, may advantageously facilitate and improve the synthesis of the framework material such as vivo protein, fat, hence it is evident that the body condition of milch cow can be improved.

The impact on milk production of cow of the 2.2 care of feed cream

5120 days milk production of cow of table

	Head number (head)	Give milk natural law (my god)	Total milk yield (kg)	Average milk production (kg/ days. head)
					Matched group	160	120	736704	38.37±5.16
Test group	160	120	880896	45.88±5.92
					Process difference^b				7.51

Result above shows: test group is compared with matched group, and head day increases milk yield 7.51 kilograms, improves 19.57%, and t checks significant difference (P < 0.05), and care of feed cream is remarkably improved the milk yield of lactating cow.

The impact on immunity of cow of the 2.3 care of feed cream

Table 6 Diseases of Cow Immunoglobulins in Serum testing result

Immunoglobulin IgA in cow serum, IgM, IgG content be the important indicator judging milch cow humoral immunity level.As seen from the experiment, cow birth stress cause that serum immune globulin IgA, IgM, IgG content sharply decline, and humoral immune function is suppressed.Test group care of feed cream can increase before cow birth after 1 week, the childbirth same day and childbirth the content of 1 week serum IgA, IgM, IgG, enhancing humoral immune function.

The impact on Diseases of Cow reproductive performance of the 2.4 care of feed cream

Table 7 Diseases of Cow reproductive performance testing result

Result of the test shows, compared with matched group, test group Diseases of Cow can be greatly shortened nearly 13 days of the time of milch cow postpartum heat, improves the conception rate 19.6% of the first feelings phase milch cow in puerperal, increase the ratio of oestrusing 17.7% of Postpartum Cows colony, be effectively improved the reproductive performance of milch cow.

The impact that Diseases of Cow puerperal disease is occurred by 2.5 care of feed cream

Table 8 Diseases of Cow puerperal disease testing result

Result above shows: test group is compared with matched group, in prevention postpartum disease of cow, the sickness rate of various disease can be significantly reduced, on average reduce 11.87-28.1%, t checks significant difference (P < 0.05), care of feed cream can significantly reduce the incidence rate of the diseases such as milch cow mastitis in puerperal, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and placenta retention, improves the health status of milch cow.

To sum up, use care of feed cream of the present invention to be remarkably improved milch cow physique, immunity production performance, reproductive performance and the various disease of prevention, effectively prevent the harm of cultivation directly or indirectly brought because of Milk cow hoof disease,

Claims

1. the milch cow care of feed cream that a permeability is strong; mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 30-50 part; Aloe extract 10-30 part; modified dietary fiber 10-15 part, water-soluble chitosan 2-8 part, plant extract 8-12 part; vegetable oil 1-5 part; Natural antioxidant 1-4 part, keratinase 0.5-1.5 part, at 0.1-1 part；

Described plant extract is can change milch cow hoof horny layer structure, to promote that care of feed cream component rapid osmotic is prepared for raw material to deep skin the natural plants that absorbs.

2. the milch cow care of feed cream that permeability as claimed in claim 1 is strong; it is characterized in that; mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 35-45 part, Aloe extract 15-25 part, modified dietary fiber 12-14 part; water-soluble chitosan 4-6 part; plant extract 9-11 part, vegetable oil 2-4 part, Natural antioxidant 2-3 part; keratinase 0.8-1.2 part, at 0.3-0.8 part.

3. the milch cow care of feed cream that permeability as claimed in claim 1 is strong; it is characterized in that; mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 40 parts, Aloe extract 20 parts, modified dietary fiber 13 parts; water-soluble chitosan 5 parts; plant extract 10 parts, vegetable oil 3 parts, the Natural antioxidant 2.5 parts; keratinase 1.0 parts, at 0.5 part.

null4. the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-3 is strong，It is characterized in that，The preparation method of described plant extract，Comprise the steps: Radix Angelicae Sinensis、Folium clausenae lansii、Flos Caryophylli、Radix Salviae Miltiorrhizae、Herba Menthae is respectively washed、Drain，10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 4.5-5.5 of mixed material quality 0.3-0.5 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-1mm,It is subsequently added into the water of ground product quality 4-6 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 8-10% obtains plant extract；

5. the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-3 is strong, it is characterised in that the preparation method of described extracts of Chinese herbal medicine; comprise the steps: to count by weight, weigh Olibanum 20-25 part, Sanguis Draxonis 18-20 part; Radix Astragali 13-16 part, Myrrha 12-15 part, Os Draconis 12-15 part; Radix Achyranthis Bidentatae 8-12 part, Cortex Acanthopancis 7-9 part, Pulvis Fumi Carbonisatus 4-6 part; Herba Portulacae 4-6 part; Radix Notoginseng 2-4 part, Cortex Dictamni 1-3 part, Cortex Meliae 1-3 part；Put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 with breast acid for adjusting pH value, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 with breast acid for adjusting pH value, enzymolysis 2-4h, finally add the mixture of 0.5-3 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:1-3, control temperature and keep 3-4h to 60-78 DEG C, filter, obtain the first filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, be then cooled to 25-35 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

6. the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-3 is strong; it is characterized in that; the preparation method of described Aloe extract; comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz; drain; it is cut into phase equal-specification; length and width is 5-10mm; the sheet of thickness 3-5mm or fourth; 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Adding the deionized water of ground product quality 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, carries out high voltage pulse electric field processing when pulse frequency 200-400Hz, then normal pressure boils 30-50min, Bag filter, filtering residue boils 40-60min, Bag filter, merging filtrate with its filtrate normal pressure of quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

7. the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-3 is strong; it is characterized in that; the preparation method of described modified dietary fiber; comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio; add its water of quality 3-7 times; room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min; then at electric field intensity 20-40kV/cm; burst length 300-500 μ s, carries out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min；Enzymolysis solution filters, and namely filtrate reduced in volume, vacuum lyophilization, micronizing obtain modified dietary fiber to particle diameter 0.1-0.3mm；

8. the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-3 is strong, it is characterised in that the described Natural antioxidant contains any one or a few in tea polyphenols, grape pip procyanidin, Herba Rosmarini Officinalis extract, Radix Glycyrrhizae extract, Folium Pruni extract and Vc.

9. the preparation method of the milch cow care of feed cream that permeability as described in as arbitrary in claim 1-8 is strong, it is characterized in that, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100-200 μm, it is subsequently adding vegetable oil, mix homogeneously, in power 200-400W, ultrasonic Treatment 18-28min is carried out when frequency 35-45KHz, finally by high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 80-100MPa, making homogenizer outlet material particle diameter is 150-450nm, obtain nanoparticle mixed liquor；Seal; room temperature natural fermentation 3-5d; then heat to 60-70 DEG C; insulation, is sequentially added into water-soluble chitosan, modified dietary fiber while stirring, fully dissolves 20-30min; it is down to room temperature; it is sequentially added into the Natural antioxidant, at and keratinase, Homogeneous phase mixing while stirring, is evaporated to paste and namely obtains the milch cow care of feed cream that permeability is strong.

10. the preparation method of the milch cow care of feed cream that permeability as claimed in claim 9 is strong, it is characterised in that the quality group of the described Natural antioxidant becomes: tea polyphenols: grape pip procyanidin: Vc=5-7:2-4:1-3.