CN105641287A

CN105641287A - Biological and environment-friendly cow hoof bath lotion and preparation method thereof

Info

Publication number: CN105641287A
Application number: CN201610100807.1A
Authority: CN
Inventors: 张玉峰
Original assignee: Ningxia Yufeng Technology Co Ltd
Current assignee: Ningxia Yufeng Technology Co Ltd
Priority date: 2016-02-24
Filing date: 2016-02-24
Publication date: 2016-06-08

Abstract

The invention discloses a biological and environment-friendly cow hoof bath lotion. A Chinese herbal medicine extract solution capable of effectively killing and inhibiting infectious pathogenic bacteria of the cow hoof disease, promoting hoof wounds to heel, effectively preventing and treating various hoof diseases and enhancing immunity and disease resistance of cows and a high-cohesiveness aloe extract capable of promoting medicine permeation and effectively repairing stratum corneum are taken as main raw materials, plant extracts capable of changing cow hoof stratum corneum structures and promoting effective ingredients such as Chinese herbal medicines, aloe and the like to quickly permeate deep layers of skin to be absorbed are added, ultrasonic dispersion, dissolution, high-pressure homogenization and cold and heat treatment technologies are organically combined, modified dietary fiber with high cohesion is scientifically compounded, trehalose, egg white, a natural antioxidant and the like which can prevent effective ingredients of components from being oxidized and improve physical and chemical stability of a hoof spray are added, and the hoof bath lotion which is high in stability, safe, efficient and free of any antibiotics and chemical harmful substances is prepared finally.

Description

A kind of biological environmental production milk cow hoof body lotion and its preparation method

Technical field

The present invention relates to milk cow and protect hoof product, be specifically related to a kind of biological environmental production milk cow hoof body lotion and its preparation method.

Background technology

In milk cattle cultivating, hoof disease, mastitis and Reproduction Disorder are cause milk cow to be forced to eliminate three big diseases, the limping of milk cow 90% causes due to hoof disease, therefore, hoof disease is the regular incidence of milk cow, and mainly calcium in daily ration, phosphorus is uneven or trace element deficiency for its cause of disease, cause cutin hoof to loosen, add that colony house environment is severe, hoof often by excrement urine, mud dipping, hoof cutin softening and fall ill. In addition, the hard object such as stone, iron filings, vegetation shred, fragments of glass, all can stab hoof soft tissue and cause infection inflammation. After milk cow suffers from hoof disease, appetite reduces, and amount of exercise reduces, and happiness is lain prone sleeping, limping, pain, occurs pain having hotness during palpation during percussion hoof. After expanding wound with poring iron, aperture at the bottom of hoof or the smelly water outflow namely having dirt black in hole, it can be seen that have ulcer between toe, be coated with the sphacelus of stench. Severe one coronet is red and swollen, and pain obviously, can not stand, and milk yield seriously declines, and reproductivity reduces, and finally eliminates by sick ox. Along with developing rapidly of China milk industry, from having introduced a large amount of high-quality milk cow, freezing seminal fluid and embryo abroad, but these high yield cows may not too adapt to domestic feeding environment and diet nutrient, cause hoof disease sickness rate to have the trend of rising, cause huge financial loss to China's milk cow production. According to statistics, in milk cattle cultivating field, China some areas, hoof disease sickness rate is about 50%, ill rear cow feeding amount reduces by 20%, milk yield reduces by 20%, because the superseded ox head number of hoof disease accounts for the 50% of total superseded ox, medical expense increases by 30%, and the financial loss caused because of hoof disease accounts for the 10% of annual profit. Therefore, prevent and treat Milk cow hoof disease, it is possible to reduce cow culling rate, significantly improve the economic benefit of China's milk cattle cultivating.

Hoof disease is a very ambiguous and general idea, is summed up it and in fact comprises a lot of disease, common are between ulcer at the bottom of hoof, hoof toe dermatitis, wart dermatitis etc. between skin proliferation, toe. produce the common cause of disease of above-mentioned hoof disease mainly contain venereal infection because of, metabolic obstacle, environmental factors and heredity factor, the measure correspondingly taked mainly carries out protecting hoof, repair hoof and clinical treatment, be hoof disease prevent or adopting to treat in any way all can relate to a series of protects hoof medicine, such as protect hoof cream, protect hoof liquid, hoof body lotion and protect hoof spraying etc., in these medicines, antibiotic medicine occupies very vast scale, but milk cow and the mankind can be produced great effect by a large amount of use antibiotic product, resistance in milk cow and mankind's body is caused to strengthen, greatly compromise milk cow, the physical health of poultry feeders and epidemic prevention personnel, breeding environment can be caused to pollute simultaneously, also can strengthen the security hidden danger of food (milk and beef).

Hoof body lotion is that the one being applicable to the hoof health care of raising milk cow colony protects hoof product, recovery stage after milk cow enters colony's epidemic prevention or repaiies hoof and clinical treatment, occur according to hoof disease or treatment degree, thoroughly clean cow hoof portion, guarantee that hoof and toe gap do not have ight soil to remain, make it clean, hoof body lotion is configured to certain density liquid, then add in special milk cow hoof bath, liquid level is 15cm at least, milk cow pass by special hoof bath namely complete hoof bath, to prevent hoof disease occurs and nursing hoof is healthy, therefore, hoof bath is the measure the most important preventing Milk cow hoof disease, hoof body lotion be prevent Milk cow hoof disease indispensable protect hoof product.

Chinese herbal medicine specimen is ruled together, cure the symptoms, not the disease a lot than Western medicine (as: tradition hoof body lotion is copper-bath or the 3-5% formalin solution of 5-10%), hoof disease can be effected a radical cure, and side effect is little, pure natural, it is easy to degraded, environmentally friendly, at present, relevant Chinese medicine protects hoof product and has been widely used in milk cattle cultivating. But, Chinese medicine kind is many, complicated component, poor as its pharmacodynamic stability of Liquid traditional Chinese medicine preparation, and based on the feature of bringing out of the breeding environment of milk cow, feeding pattern, hoof disease and prevent and remedy measures, the result for the treatment of of bound drug, the disease resistance of hoof body lotion, adhesivity and perviousness etc. are still the emphasis of concern.

Chinese patent CN104825486A discloses a kind of medicinal composition being used for the treatment of cow hoof disease, comprises quadrol dihydroiodide and copper sulfate, and the mass mixing ratio of quadrol dihydroiodide and copper sulfate is 1:2��2:3. Quadrol dihydroiodide can react with copper sulfate, discharge free iodine, the atomic iodine of unbound state has superpower oxygenizement, it is possible to makes protein denaturation and destroys the membrane structure of pathogenic agent, thus reaches preventing infection and the object for the treatment of cow hoof disease. It is the surface oxidation sterilization utilizing the atomic iodine of strong oxidation to carry out disease hoof that composition in above-mentioned publication mainly acts on, only carry out disinfecting of surface, can not effect a radical cure, and intractable fungi effect is very micro-, osmosis is weak, and result for the treatment of is undesirable; Simultaneously also will with antibiotics and use, produce side effect, and easily there is chemical reaction in composition, not easily circulates, storage and using.

Chinese patent CN102552339A discloses a kind of nanoemulsion medicine being used for the treatment of foot rot, this nano-emulsion particle diameter is between 1��100nm, it is made up of the raw material of following component: pine evaporates oil 0.1%��10.0%, phenylacrolein 0.1%��6.0%, isothymol 0.1%��6.0%, white oil of camphor 0.1%��5.0%, clobetasol propionate 0.01%��0.1%, tensio-active agent 18.0%��35.0%, cosurfactant 0��7.0%, surplus are distilled water, and the mass percent sum of mentioned component is 100%. This nano-emulsion has and dissolves that cutin, antipruritic, anti-inflammatory, convergence, partly sterilised be anticorrosion and the effect such as penetration enhancement, is mainly used in treatment animal foot rot. Invention increases the solubleness of medicine, it is to increase medicine stability and bioavailability, decrease drug dose, transdermal rate is higher than general formulations such as paste, and with low cost, has wide market outlook in field of veterinary. The composite medicament categories of above-mentioned disclosed breast medicine is less, effect is single, can not the hoof disease of complete treatment milk cow, and evaporate oil, white oil of camphor etc. containing the pine that glucocorticoids medicine clobetasol propionate and toxicity are bigger, easily infiltrate from wound, produce certain side effect.

Chinese patent CN102100750A discloses a kind of traditional Chinese medicine lotion and its preparation method, belongs to herbal medicine product preparation method's technical field. Pharmaceutical cpd by by quality is: indigo naturalis 0.5-2 part; Realgar 1-3 part; Alumen 1-3 part; Coptis 0.5-2 part; Rheum officinale 1-3 part; Forge borax 0.5-2 part; Catechu 0.5-2 part; Peppermint 1-3 part is formed, each for medicine except peppermint component the is added 10 times cold water soak of medicinal material amount 30 minutes, decoct and boil 20 minutes, add peppermint to decoct 10 minutes again, filter, the dregs of a decoction the add 8 again times soak by water of medicinal material amount 30 minutes, filter, merge twice filtrate, it is evaporated to the concentrated medicament of every 1ml liquid containing crude drug in whole 2g, adds the mature vinegar of equivalent after cooling and get final product. This preparation method's production technique is simple, and facility investment is few, and product price is cheap, is suitable for suitability for industrialized production. Above-mentioned publication Chinese medicine adopts traditional decocting medicine, utilization rate of active components is low, loss is big, is sprayed to disease hoof surface bad adhesion, it is easy to come off, osmosis is poor, the focus on below top layer can not be treated, and containing toxic bigger realgar, highly toxic substance arsenic can be produced under decoction process and light conditions, using and there is potential safety hazard with storage process, stability is poor.

Chinese patent CN104258319A discloses a kind of medicinal herb spray treating cattle footrot, is loaded body obtained by Fruit of Cherokee Rose, Yunnan Caulis Spatholobi, Herba Violae japonicae, tarragon, rosin, Turkey-galls, rhizoma zingiberis after extracting. Sick ox is carried out spray delivery by the product of the present invention, 2 times on the one, is used in conjunction 5��7, can effectively control cattle footrot, it may also be useful to convenient, cost is low, noresidue, biological safety height. Above-mentioned publication Chinese medicine kind is few, the foot rot in Milk cow hoof disease can only be treated, can not the various hoof disease of prevention and therapy comprehensively, and adopt traditional decocting medicine, utilization rate of active components is low, loss is big, it is sprayed to disease hoof surface bad adhesion, it is easy to coming off, osmosis is poor, the focus on below top layer can not be treated, also add the chemical substance such as sanitas, stablizer as carrier simultaneously.

Chinese patent CN102846489B discloses a kind of Polyhaxemethylenguanidine Hydrochloride iodine dipping liquid and its preparation method, its components in mass portion number comprises: iodine 0.2-2 part, potassiumiodide 0.5-5 part, Potassium Iodate 0.1-1 part, tripoly phosphate sodium STPP 0.1-1 part, Polyhaxemethylenguanidine Hydrochloride 5-50 part, stain remover 1-20 part, skin-protecting agent 0.5-5 part, citric acid 0.05-1 part, water 15-92.55 part: its preparation comprises: Polyhaxemethylenguanidine Hydrochloride stain remover and skin-protecting agent are added to the water respectively, it is stirred in 45-70 DEG C and mixes, then iodine is added, potassiumiodide, Potassium Iodate, stir at 45-70 DEG C, it is made fully to dissolve, add tripoly phosphate sodium STPP and citric acid again, at 45-70 DEG C, it is stirred to each component dissolves completely, to obtain final product. the available iodine content of the dipping liquid of the present invention is big, fungicidal effectiveness height, good stability, is applicable to pasture and uses, the preparation method of the present invention is simple to operate, is produced on a large scale. above-mentioned disclosed body lotion is mainly used in sterilization, can not well prevent Milk cow hoof disease, and contain more chemical substance, contaminate environment.

Chinese patent CN104257684A discloses a kind of for preventing nipple dipping liniment and its preparation method of mastadenitis of cow. Nipple dipping liniment wherein is prepared from by more than ten raw materials such as membrane-forming agents. The advantage of the present invention has three aspects, and first is add membrane-forming agent in dipping liquid, forms barrier between nipple and environment. 2nd is add skin-protecting agent in dipping liquid, protection nipple skin. 3rd is sterilization, film forming and skin care are organically combined, and increases the function of nipple dipping sterilizing agent, and omnibearing protection nipple, prevents the generation of mastadenitis of cow more effectively. Above-mentioned disclosed dipping liquid mainly prevents mastadenitis of cow, can not prevent Milk cow hoof disease.

To sum up, prepare a kind of can prevent comprehensively milk cow all kinds hoof disease, stability, adhesivity and perviousness strong, safe and efficient, be the urgent needs of numerous raisers without the hoof body lotion of any microbiotic and chemistry toxic substance, be also responsibility and the pursuit of those skilled in the art.

Summary of the invention

Technical problem solved by the invention is the defect overcoming existing milk cow hoof body lotion, effectively to kill and suppress the infectious pathogenic bacteria of Milk cow hoof disease and promote hoof wound healing, it is possible to effectively prevent and treat multiple hoof disease, improve the extracts of Chinese herbal medicine of milk cow autoimmunity and disease resistance and have promote drug osmotic, effectively repair stratum corneum, the Aloe extract of strong cohesive property be main raw material, science compound highly effective bonding modified dietary fiber, milk cow hoof stratum corneum structure can be changed and promote the plant milk extract that the effective constituent rapid osmotic such as herbal medicine absorb to deep skin, science is composite simultaneously has anti-freezing, high temperature resistance high temperature, the trehalose of bioactive ingredients liquid stable and component effective constituent can be protected from oxidation in the hoof the guaranteed body lotion of resist drying performance, improve egg white and the natural antioxidants of hoof body lotion physical and chemical stability, and with the addition of and promote that hoof body lotion bonds and the M-Zyme that permeates, at etc., final obtained a kind of stability is strong, can complete treatment milk cow all kinds hoof disease, bonding is firmly, perviousness is strong, safe and efficient, without the hoof body lotion of any microbiotic and chemistry toxic substance, reduce milk cattle cultivating cost, improve fanning economics, add the security of milk cow deep-processed food, protect environment.

In order to achieve the above object, the present invention adopts following technical scheme:

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number:

Extracts of Chinese herbal medicine 60-80 part, Aloe extract 40-60 part, plant milk extract 20-40 part, modified dietary fiber 6-12 part, trehalose 5-11 part, egg white 3-9 part, natural antioxidants 5-11 part, M-Zyme 0.5-1.5 part, at 0.1-1 part;

Preferably, a kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number:

Extracts of Chinese herbal medicine 65-75 part, Aloe extract 45-55 part, plant milk extract 25-35 part, modified dietary fiber 8-10 part, trehalose 7-9 part, egg white 5-7 part, natural antioxidants 7-9 part, M-Zyme 0.8-1.2 part, at 0.4 0.6 parts;

More preferably, a kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number:

Extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant milk extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts, egg white, natural antioxidants 8 parts, M-Zyme 1.0 parts, at 0.5 part;

Further, described extracts of Chinese herbal medicine effectively kills and suppress the infectious pathogenic bacteria of Milk cow hoof disease, and there is promoting blood circulation and stopping pain, detumescence myogenic, eliminating dampness hemostasis, kill worm removing toxic substances, hygroscopic, sore, antispastic, heat of dispelling, it is possible to it is prepared by raw material for effectively preventing and treat multiple hoof disease, improving the Chinese medicine of milk cow autoimmunity and disease resistance;

Preferably, the preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, take frankincense 20-25 part, dragon's blood 18-20 part, Radix Astragali 13-16 part, myrrh 12-15 part, keel 12-15 part, Radix Achyranthis Bidentatae 8-12 part, Wu Chia Pee 7-9 part, Plant Soot 4-6 part, purslane 4-6 part, pseudo-ginseng 2-4 part, Root-bark of Densefruit Pittany 1-3 part, Cortex Meliae 1-3 part; Put in the Ultrasonic Cleaners filling 0.1-0.3% sodium hydrogen carbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixture, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 by lactic acid adjust ph, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic-assisted extraction; Then the prozyme adding mixture gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixture 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Adding the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the 2nd filtrate; First filtrate and the 2nd filtrate being merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtains extracts of Chinese herbal medicine;

Described prozyme is dextranase, pentosanase, zytase, aspartic protease, tannase 5-7:2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described Aloe extract take aloe as main raw material, the waste residues such as pericarp or hawthorn, beet, apple such as shaddock that the composite pectin content of science is higher, lemon, orange, citrus, grape extract through ultrasonic cleaning, high-voltage pulse, hot water extraction and obtained;

Preferably, the preparation method of described Aloe extract, comprise the steps: fresh aloe, orange peel, Pericarpium Vitis viniferae, hawthorn pomace to be put into the Ultrasonic Cleaners that 1.2% sodium hydrogen carbonate solution is housed respectively and clean 5-10min in 200W, 40KHz, drain, being cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min; Pulverizing immediately, particle diameter is 0.1-1mm; Add crushed material quality 3-5 deionized water doubly, mix, be 2-3 by citric acid adjust ph, at strength of electric field 35-45kV/cm under room temperature, in burst length 400-600 �� s, carrying out pulsed electric field process when pulse-repetition 200-400Hz, then normal pressure boils 30-50min, Bag filter, filter residue boils 40-60min, Bag filter, merging filtrate with the filtrate normal pressure of its quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

Further, described plant milk extract be taking can change milk cow hoof stratum corneum structure, promote hoof body lotion component rapid osmotic to deep skin and the natural phant absorbed prepare as raw material. Such as: Radix Angelicae Sinensis, Leaf of Chinese Wampee, cloves, the red sage root, peppermint etc.;

Preferably, the preparation method of described plant milk extract, comprise the steps: Radix Angelicae Sinensis, Leaf of Chinese Wampee, cloves, the red sage root, peppermint is cleaned respectively, drain, 10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio, add the wetting 3-8h of lactic acid that mixture quality 0.3-0.5 pH value doubly is 4.5-5.5, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing bed thickness 3-5cm, crushed material particle diameter 0.5-1mm, then crushed material quality 4-6 water doubly is added, it is 3.5-5.5 by lactic acid adjust ph, at strength of electric field 25-35kV/cm under room temperature, burst length 300-500 �� s, pulsed electric field process 20-30min is carried out when pulse-repetition 200-300Hz, then carry out microwave exposure when power 150-300W in room temperature and extract 15-20min, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic-assisted extraction, add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate decompression concentrates to solid content is that namely 8-10% obtains plant milk extract,

Described mixed enzyme is cellulase, hemicellulase, polygalacturonase, tannase, proteolytic enzyme 3-5:3-5:2-4:1-3:0.3-0.5 Homogeneous phase mixing in mass ratio.

Further, described modified dietary fiber is the impact with strong retentiveness, swelling property, thickening property, adsorptivity and acid and alkali, alkali, salt obtained through physics, chemistry or the process of biological method by food fibre, significantly improves cohesiveness and the physical and chemical stability of milk cow hoof body lotion;

Preferably, described modified fibre is obtained through biological enzyme enzymolysis by one or more in inulin, apple fibre, oat fibre, wheat fiber;

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, wheat fiber, apple fibre, oat fibre 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at strength of electric field 20-40kV/cm, in burst length 300-500 �� s, pulsed electric field process 10-15min when pulse-repetition 200-400Hz, is carried out; It is 4.5-6.5 by lactic acid adjust ph, adds the biological enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min; Enzymolysis solution filters, and it is that namely 5-8% obtains modified dietary fiber that filtrate decompression concentrates to solid content;

Described biological enzyme is cellulase, dextranase, zytase, polygalacturonase 5-7:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.

Further, described natural antioxidants contains any one or a few in tea-polyphenol, grape pip procyanidin, rosemary extract, Radix Glycyrrhizae extract, apricot leaf extract and Vc;

Preferably, the quality group of described natural antioxidants becomes: grape pip procyanidin: tea-polyphenol: rosemary extract=4-6:2-4:1-3.

Another object of the present invention is to provide the preparation method of above-mentioned biological environmental production milk cow hoof body lotion, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, outlet mixture particle diameter is made to reach 100-200 ��m, then by high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 100-120MPa, make clarifixator outlet material particle diameter be 100-150nm, obtain nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 12-20min in rotating speed 600-720r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution is to 65-75 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 130-150r/min, fully dissolve 20-30min, stop stirring, heat up, normal pressure boils 5-10min, naturally room temperature it is down to, continue to be cooled to-1.5-0.5 DEG C, insulation 1-3h, repeat above-mentioned normal pressure to boil and low temperature insulation operation 2-4 time, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 100-120r/min, Homogeneous phase mixing, in power 200-400W, ultrasonication 10-30min is carried out when frequency 35-45KHz, simultaneously at strength of electric field 35-45kV/cm, burst length 600-800 �� s, pulsed electric field process is carried out when pulse-repetition 400-600Hz, last in 0.2-0.4 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The using method of biological environmental production milk cow hoof body lotion of the present invention: prevent according to hoof disease or treat requirement, thoroughly clean cow hoof portion, guarantee that hoof and toe gap do not have ight soil to remain, make it clean, hoof body lotion being configured to the liquid that mass percent concentration is 4-8%, then adds in special milk cow hoof bath, liquid level is 15cm at least, milk cow hoof soaks 20-30min in special hoof bath and namely completes a hoof bath, changes hoof body lotion when same hoof bath hoof bathes 200 times.

In the prevention cycle: liquor strength 4-6%, every day is sooner or later each continuous 3 days is once an epidemic prevention cycle, quarterly an epidemic prevention cycle;

Treatment cycle: liquor strength 6-8%, every day, sooner or later respectively once it 4 days was a treatment cycle that continuous hoof is bathed;

Useful effect:

The present invention is effectively to kill and suppresses the infectious pathogenic bacteria of Milk cow hoof disease and promotes hoof wound healing, can effectively prevent and treat multiple hoof disease, improve the extracts of Chinese herbal medicine of milk cow autoimmunity and disease resistance and there is promotion drug osmotic, effectively repair stratum corneum, the Aloe extract of strong cohesive property is main raw material, interpolation can change milk cow hoof stratum corneum structure and promote herbal medicine, the plant milk extract that the effective constituent rapid osmotic such as aloe absorb to deep skin, by ultrasonic disperse, dissolving technology and high pressure homogenization technique organically combine, further by material effective component particle nanometer, it is prepared into more stable, the more easily nanoparticle mixing solutions of infiltration and absorption, the not only modified dietary fiber of science compound highly effective bonding, and with the addition of component effective constituent can be protected from oxidation, improve the trehalose of hoof body lotion physical and chemical stability, egg white and natural antioxidants, with the addition of simultaneously and promote that hoof body lotion bonds and the M-Zyme that permeates, at, final obtained a kind of stability is strong, can complete treatment milk cow all kinds hoof disease, bonding is firmly, perviousness is strong, safe and efficient, without the hoof body lotion of any microbiotic and chemistry toxic substance, it take natural phant as raw material, adopt machining at low temperature technology, after particularly adopting cold-heat treatment, hoof body lotion transparency, stability is stronger, the quality guaranteed period extending hoof body lotion is 42-54 month, 18-30 month is extended than commercially available (24 months), do not add any chemistry toxic substance, prevention and therapy Milk cow hoof disease process does not use any microbiotic, reduce milk cattle cultivating cost, improve fanning economics, add the security of milk cow deep-processed food, protect environment. experiment proves (embodiment 7-10): 1) to compare visual appearance with commercially available hoof body lotion better for hoof body lotion of the present invention, clarity height, along with the prolongation of time, hoof body lotion liquid phase is relatively unstable, according to use and the drug effect standard of hoof body lotion, the hoof body lotion quality guaranteed period of the present invention can reach 42-54 month, extends 18-30 month than commercially available (24 months), also illustrate that inner quality of the present invention is better simultaneously, drug effect safety, stable. 2) hoof body lotion of the present invention can effectively prevent the generation of the various body disease of milk cow, especially ulcer at the bottom of foot rot, hoof, wart dermatitis and other hoof disease is had effect of preventing epidemic preferably, and compared with commercially available hoof body lotion, its hoof disease incidence reduces by 39.14%. also illustrate that hoof body lotion of the present invention has good perviousness, cohesiveness, high efficiency simultaneously. 3) various Milk cow hoof disease can fast, effectively be treated by hoof body lotion of the present invention, cow hoof disease is had antibacterial anti-infection, detoxifies and restrain, remove necrosis and promote granulation and promote the effect of wound healing, and perviousness is strong compared with commercial hoof body lotion, drug effect height, cohesiveness are strong. when medication 7 days, its curative ratio reached 65.7-80%, has always had efficiency to reach 88.6-94%, 49.7-66.7% higher than control group (13.3-16%) and (55-80%) and 8.6-39% respectively, when medication 14 days, its curative ratio reached 90-96%, has always had efficiency to reach 96.7-100%, 14-36% higher than control group (60-76%) and (70-86%) 10.7-30% respectively, reach same therapeutic efficiency, treatment time at least shortens 7 days, particularly to wart dermatitis, the hoof disease result for the treatment of of foot rot and other type is best, illustrate simultaneously, therapeutic process is not when using antibiotics assisting therapy, hoof body lotion treatment time of the present invention is short, efficiency height, not only reduce milk cattle cultivating cost, add fanning economics, and improve the food safety of milk cow deep processed product, also protect breeding environment simultaneously, stop because using microbiotic, the potential safety hazard that chemistry and toxic substance produce, for application hoof body lotion treatment Milk cow hoof disease opens a new green shortcut.4) trend that milk cow body condition is marked and is significantly improved by hoof body lotion of the present invention, milk cow can be improved to the digestion of nutritive substance, absorption and metabolism, be conducive to promoting and improving the synthesis of the framework material such as albumen, fat in body, obviously can improve the body condition of milk cow. 5) test-results shows: test group is compared with control group, and head day increases milk yield 7.38 kilograms, it is to increase 19.23%, t checks significant difference (P < 0.05), and hoof body lotion can significantly improve the milk yield of lactating cows. 6) in cow serum, the content of Immunoglobulin IgA, IgM, IgG is the important indicator judging milk cow humoral immunity level. As seen from the experiment, cow birth stress cause serum immune globulin IgA, IgM, IgG content sharply to decline, and humoral immune function is suppressed. Test group hoof body lotion can increase before cow birth 1 week, the childbirth same day and point 1 week puerperium serum IgA, IgM, IgG content, enhancing humoral immune function. 7) compared with control group, test group milk cow perinatal period can shorten nearly 15 days of the time that first time in milk cow postpartum oestruses greatly, improve the conception rate 20.5% of feelings in postpartum first phase milk cow, increase the ratio of oestrusing 18.5% of Postpartum Cows colony, effectively improve the reproductive performance of milk cow. 8) test group is compared with control group, in prevention postpartum disease of cow, can significantly reduce the sickness rate of various disease, on average reduce 10-28.7%, t checks significant difference (P < 0.05), hoof body lotion can significantly reduce the incidence of the diseases such as milk cow mastitis in postpartum, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and retention of placenta, improves the healthy state of milk cow.

Concrete know-why is as follows:

1. the extracts of Chinese herbal medicine that prepared by the present invention effectively kills and suppress the infectious pathogenic bacteria of Milk cow hoof disease, and has promoting blood circulation and stopping pain, detumescence myogenic, eliminating dampness is stopped blooding, kill worm removing toxic substances, hygroscopic, sore, antispastic, dispel heat, it is possible to effectively prevent and treat multiple hoof disease, the Chinese medicine improving milk cow autoimmunity and disease resistance is that raw material is through ultrasonic cleaning, ultrasonic assistant microwave extraction, prepared by low temperature enzymolysis and water extraction alcohol extracting, extracting solution active constituent content height, medicinal material utilization ratio height, extraction time is short, extraction yield height, extraction cost is low, drug effect highly effective and safe, can effectively prevent and prevent various Milk cow hoof disease, be different from prior art, and while reaching better prevention and therapy effect, science of the present invention is composite various conventional, the natural Chinese medicinal herb effective and property of medicine is soft, containing calomel mercurous chloride, realgar, the severe toxicity Chinese medicine such as yellow lead, does not use any pharmaceutical chemicals and microbiotic yet, prevents it from entering blood circulation by wound, further increase milk, the food safety of the deep processed products such as beef, the security of the security that the medicine that simultaneously improve poultry feeders uses and breeding environment, protects environment. above-mentioned drug effect is the result of each herbal component synergy, not the superposition of single component drug effect.

2. the Aloe extract of the preparation of the present invention take aloe as main raw material, the composite orange peel of science, Pericarpium Vitis viniferae, hawthorn pomace is through ultrasonic cleaning, high-voltage pulse extracts, hot water extraction and obtained, remain Aloe gel wherein to greatest extent, pectin, rhabarberone, Aloin A, traumatin and glycan peptide sweet dew, the multiple bioactive ingredients such as Aloe polysaccharide, its viscosity is considerably beyond the viscosity of single Aloe gel and pectin, substantially increase the cohesiveness of hoof body lotion, the multiple bioactive ingredients the more important thing is in aloe can promote wound healing and restoration ecosystem and not stay scar, powerful reparation stratum corneum, anti-virus infection, Pesticidal and sterilizing, sun-proof anticancer, anti-ageing removing toxic substances, there is multiple efficacies, also can promote and carry other effective constituents and penetrate into hoof deep layer, it is to increase the osmotic efficiency of itself and herbal medicine, shorten penetration time, work in coordination with other compositions, such as: modified dietary fiber, plant milk extract, egg white etc., its drug effect and use properties are stronger, are applicable to very much the drug effect of milk cow hoof body lotion, security and administering mode and method requirement.

3. changing, the effective constituent rapid osmotic such as milk cow hoof stratum corneum structure, promotion herbal medicine to deep skin and absorbs the plant milk extract that prepared by the present invention, to effect a radical cure the natural phant of focus as raw material, science composite and through cleaning, wetting, freezing and pulverizing, pulsed electric field extraction, the extract at low temperature technique such as ultrasonic assistant microwave extraction, biological enzymolysis prepare, such as: Radix Angelicae Sinensis, Leaf of Chinese Wampee, cloves, the red sage root, peppermint etc.; Its active constituent content height; employing natural plant extracts is permeate promotor; instead of the chemistry permeate promotors such as the nitrogen ketone of prior art, dimethyl sulfone, tween, propylene glycol, ethanol completely and urge saturating effect more remarkable; not only increase the perviousness of hoof body lotion; and improve the pharmacological property of hoof body lotion; add the safety in utilization of hoof body lotion simultaneously, protect environment. Working in coordination with extracts of Chinese herbal medicine, its drug effect, security and usability are stronger.

4. the egg white that the present invention uses, after high-speed stirring, its volume can increase at least 6 times, it is a kind of excellent solidifying wadding agent, flocculation material can be formed with part (such as: the Chinese medicine albumen) high molecular weight protein " combination " in component, be conducive to improving the stability in storage of hoof body lotion, egg white has strong convergence effect simultaneously, can promote wound healing, anti-inflammatory, the pain that disappears pain relieving, prevent the effects such as suppuration, and egg white is easily in keratoderma film forming, it is to increase the adhesivity of hoof body lotion and efficacy. Better with other component synergistic action effects such as at, M-Zyme, modified dietary fibers.

5. the impact with strong retentiveness, swelling property, thickening property, adsorptivity and acid and alkali, alkali, salt that food fibre is obtained by the modified dietary fiber that prepared by the present invention through physics, chemistry or the process of biological method, significantly improves cohesiveness and the physical and chemical stability of milk cow hoof body lotion; High adsorption capacity, through modified, the specific surface area of Mierocrystalline cellulose increases, grid structure is enriched, adsorptive power strengthens, and further enhancing the cohesiveness of hoof body lotion, and powerful bury function can prevent breeding environment (oxygen, temperature, illumination, water-activity etc.) factor on the impact of product drug effect, stabilize the property of medicine of hoof body lotion, extend quality guaranteed period of hoof body lotion and prevent and time limit of service in therapeutic process, use the property of medicine. Working in coordination with egg white, trehalose, effect is better.

6. science of the present invention is composite a small amount of M-Zyme and at, can decompose milk cow hoof stratum corneum appropriateness, increase effective ingredient rapid osmotic to internal layer, shorten treatment time and increase result for the treatment of; The stratum corneum that appropriateness is decomposed simultaneously is more prone to and Chinese herbal medicine effective ingredients, Aloe gel, pectin, egg white, modified dietary fiber bonding, stability when further enhancing the cohesiveness of hoof body lotion and use, difficult drop-off.

7. science of the present invention is composite trehalose, natural antioxidants, can effectively prevent the biological activitys such as M-Zyme, at, extracts of Chinese herbal medicine, Aloe extract, plant milk extract and effective constituent oxidation, high temperature, freezing and lose activity and effect, enhance the stability of hoof body lotion, drug effect, perviousness and cohesiveness, finally improve security and the usability of hoof body lotion, better with the synergistic action effect such as modified dietary fiber, egg white.

8. preparation method of the present invention overcomes tradition and existing hoof body lotion High Temperature Sterilization, process time is long, efficiency is low, drug effect is low, the defects such as energy consumption height, omnidistance employing machining at low temperature technology, effectively prevent the loss of material effective component, by ultrasonic dissolution, dispersion, high-pressure homogeneous, the technology such as pulsed electric field organically combine, further enhancing the synergistic effect of material effective component and the cohesiveness of hoof body lotion, perviousness and absorptivity, after particularly adopting cold-heat treatment, hoof body lotion transparency, stability is stronger, the quality guaranteed period extending hoof body lotion is 42-54 month, 18-30 month is extended than commercially available (24 months), shorten the process time, improve working efficiency, reduce production cost, improve quality product, improve fanning economics, add the security of milk cow deep-processed food, protect environment.

It should be noted that the medicinal effect of hoof body lotion of the present invention is the result that each component is mutually worked in coordination with, interacted, the not superposition of simple raw material function, the science of each feed composition is composite and extracts the effect produced considerably beyond the superposition of each single group of part function and effect, has advanced preferably and practicality.

Embodiment

The present invention is described below by specific embodiment. Unless stated otherwise, technique means used in the present invention is method known in those skilled in the art. In addition, embodiment is interpreted as illustrating property, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claim book. To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change or the changes that carry out the material component in these embodiments and consumption also belong to protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of extracts of Chinese herbal medicine:

The preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, takes frankincense 23 parts, dragon's blood 19 parts, the Radix Astragali 15 parts, myrrh 14 parts, keel 14 parts, Radix Achyranthis Bidentatae 10 parts, Wu Chia Pee 8 parts, Plant Soot 5 parts, purslane 5 parts, pseudo-ginseng 3 parts, Root-bark of Densefruit Pittany 2 parts, Cortex Meliae 2 parts; Put in the Ultrasonic Cleaners filling 0.2% sodium hydrogen carbonate solution and clean 12min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtains mixture, control temperature 80 DEG C keeps 3h, then it is cooled to 45 DEG C, it is 4.0 by lactic acid adjust ph, carry out microwave extraction 12min when power 200W, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic-assisted extraction; Then the prozyme adding mixture gross weight 1.0% carries out enzymolysis, it is 6.2 by lactic acid adjust ph, enzymolysis 3h, finally add the mixture of mixture 2 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:2, control temperature to 70 DEG C keeps 3.5h, filters, obtains the first filtrate; Adding the water of filter residue 2 times of weight, control temperature 90 DEG C keeps 2h, is then cooled to 30 DEG C, filters, obtains the 2nd filtrate; First filtrate and the 2nd filtrate being merged according to mass ratio 3:2, Homogeneous phase mixing, obtains extracts of Chinese herbal medicine;

Described prozyme is dextranase, pentosanase, zytase, aspartic protease, tannase 6:3:3:2:2 Homogeneous phase mixing in mass ratio.

2. the preparation of Aloe extract:

The preparation method of described Aloe extract, comprise the steps: fresh aloe, orange peel, Pericarpium Vitis viniferae, hawthorn pomace to be put into the Ultrasonic Cleaners that 1.2% sodium hydrogen carbonate solution is housed respectively and clean 8min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 23:4:3:2 Homogeneous phase mixing, then in-23 DEG C of freezing 20min; Pulverizing immediately, particle diameter is 0.5mm; Add the deionized water of crushed material quality 4 times, mix, be 2.5 by citric acid adjust ph, at strength of electric field 40kV/cm under room temperature, in the burst length 500 �� s, carrying out pulsed electric field process when pulse-repetition 300Hz, then normal pressure boils 40min, Bag filter, the filter residue filtrate normal pressure of its quality 2 times boils 50min, Bag filter, merging filtrate, it is 8% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

3. the preparation of plant milk extract:

The preparation method of described plant milk extract, comprise the steps: Radix Angelicae Sinensis, Leaf of Chinese Wampee, cloves, the red sage root, peppermint is cleaned respectively, drain, 11:8:8:5:2 Homogeneous phase mixing in mass ratio, the pH value adding mixture quality 0.4 times is the wetting 5h of lactic acid of 5.0, pulverize immediately after-20 DEG C of freezing 1.5h, freezing bed thickness 4cm, crushed material particle diameter 0.8mm, then the water of crushed material quality 5 times is added, it is 4.5 by lactic acid adjust ph, at strength of electric field 30kV/cm under room temperature, burst length 400 �� s, pulsed electric field process 25min is carried out when pulse-repetition 200-300Hz, then carry out microwave exposure when power 200W in room temperature and extract 18min, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic-assisted extraction, add the mixed enzyme of extracting solution quality 1.0%, in 50 DEG C of enzymolysis 40min, enzymolysis solution filters, filtrate decompression concentrates to solid content is 9% namely obtain plant milk extract,

Described mixed enzyme is cellulase, hemicellulase, polygalacturonase, tannase, proteolytic enzyme 4:4:3:2:0.4 Homogeneous phase mixing in mass ratio.

4. the preparation of modified dietary fiber:

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, wheat fiber, apple fibre, oat fibre 7:4:3:2 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 200W, 35KHz condition supersound extraction 12min, then at strength of electric field 30kV/cm, in the burst length 400 �� s, when pulse-repetition 300Hz, carry out pulsed electric field process 12min; It is 5.5 by lactic acid adjust ph, adds the biological enzyme of mixture quality 0.5%, in 50 DEG C of enzymolysis 35min; Enzymolysis solution filters, and it is 6% namely obtain modified dietary fiber that filtrate decompression concentrates to solid content;

Described biological enzyme is cellulase, dextranase, zytase, polygalacturonase 6:3:2:1.5 Homogeneous phase mixing in mass ratio.

Extracts of Chinese herbal medicine that following examples 2-6 uses, Aloe extract, plant milk extract, modified dietary fiber are embodiment 1 and prepare, and other is commercial commodity, and wherein trehalose, natural antioxidants, M-Zyme, at are powdery.

Embodiment 2

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant milk extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts, egg white, natural antioxidants 8 parts, M-Zyme 1.0 parts, at 0.5 part;

The quality group of described natural antioxidants becomes: grape pip procyanidin: tea-polyphenol: rosemary extract=5:3:2;

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, make outlet mixture particle diameter reach 150 ��m, then by high pressure homogenizer in temperature 65 DEG C, circulate 3 times when pressure 110MPa so that clarifixator outlet material particle diameter is 130nm, obtains nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 16min in rotating speed 660r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution to 70 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 140r/min, fully dissolve 25min, stop stirring, heat up, normal pressure boils 8min, naturally room temperature it is down to, continue to be cooled to-1 DEG C, insulation 2h, repeat above-mentioned normal pressure to boil and operate 3 times with low temperature insulation, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 110r/min, Homogeneous phase mixing, in power 300W, ultrasonication 20min is carried out when frequency 40KHz, simultaneously at strength of electric field 40kV/cm, burst length 700 �� s, pulsed electric field process is carried out when pulse-repetition 500Hz, last in 0.3 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The hoof body lotion quality guaranteed period prepared through aforesaid method is 54 months.

Embodiment 3

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 65 parts, Aloe extract 45 parts, plant milk extract 25 parts, modified dietary fiber 8 parts, trehalose 7 parts, 5 parts, egg white, natural antioxidants 7 parts, M-Zyme 0.8 part, at 0.4 part;

The quality group of described natural antioxidants becomes: grape pip procyanidin: tea-polyphenol: rosemary extract=4:2:1;

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, make outlet mixture particle diameter reach 100 ��m, then by high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 100MPa so that clarifixator outlet material particle diameter is 100nm, obtains nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 12min in rotating speed 600r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution to 65 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 130r/min, fully dissolve 20min, stop stirring, heat up, normal pressure boils 5min, naturally room temperature it is down to, continue to be cooled to-1.5 DEG C, insulation 1h, repeat above-mentioned normal pressure to boil and operate 2 times with low temperature insulation, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 100r/min, Homogeneous phase mixing, in power 200W, ultrasonication 10min is carried out when frequency 35KHz, simultaneously at strength of electric field 35kV/cm, burst length 600 �� s, pulsed electric field process is carried out when pulse-repetition 400Hz, last in 0.2 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The hoof body lotion quality guaranteed period prepared through aforesaid method is 52 months.

Embodiment 4

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 75 parts, Aloe extract 55 parts, plant milk extract 35 parts, modified dietary fiber 10 parts, trehalose 9 parts, 7 parts, egg white, natural antioxidants 9 parts, M-Zyme 1.2 parts, at 0.6 part;

The quality group of described natural antioxidants becomes: grape pip procyanidin: tea-polyphenol: rosemary extract=6:4:3;

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, make outlet mixture particle diameter reach 200 ��m, then by high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 120MPa so that clarifixator outlet material particle diameter is 150nm, obtains nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 20min in rotating speed 720r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution to 75 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 150r/min, fully dissolve 30min, stop stirring, heat up, normal pressure boils 10min, naturally room temperature it is down to, continue to be cooled to-0.5 DEG C, insulation 3h, repeat above-mentioned normal pressure to boil and operate 4 times with low temperature insulation, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 120r/min, Homogeneous phase mixing, in power 400W, ultrasonication 30min is carried out when frequency 45KHz, simultaneously at strength of electric field 45kV/cm, burst length 800 �� s, pulsed electric field process is carried out when pulse-repetition 600Hz, last in 0.4 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The hoof body lotion quality guaranteed period prepared through aforesaid method is 50 months.

Embodiment 5

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 60 parts, Aloe extract 40 parts, plant milk extract 20 parts, modified dietary fiber 6 parts, trehalose 5 parts, 3 parts, egg white, tea-polyphenol 5 parts, M-Zyme 0.5 part, at 0.1 part;

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, make outlet mixture particle diameter reach 100 ��m, then by high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 100MPa so that clarifixator outlet material particle diameter is 100nm, obtains nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 12min in rotating speed 720r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution to 75 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 130r/min, fully dissolve 30min, stop stirring, heat up, normal pressure boils 5min, naturally room temperature it is down to, continue to be cooled to-0.5 DEG C, insulation 1h, repeat above-mentioned normal pressure to boil and operate 4 times with low temperature insulation, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 100r/min, Homogeneous phase mixing, in power 400W, ultrasonication 30min is carried out when frequency 35KHz, simultaneously at strength of electric field 35kV/cm, burst length 800 �� s, pulsed electric field process is carried out when pulse-repetition 400Hz, last in 0.2 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The hoof body lotion quality guaranteed period prepared through aforesaid method is 42 months.

Embodiment 6

A kind of biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 80 parts, Aloe extract 60 parts, plant milk extract 40 parts, modified dietary fiber 12 parts, trehalose 11 parts, 9 parts, egg white, grape pip procyanidin 11 parts, M-Zyme 1.5 parts, at 1 part;

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, make outlet mixture particle diameter reach 200 ��m, then by high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 120MPa so that clarifixator outlet material particle diameter is 150nm, obtains nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 20min in rotating speed 600r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution to 65 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 150r/min, fully dissolve 20min, stop stirring, heat up, normal pressure boils 10min, naturally room temperature it is down to, continue to be cooled to-1.5 DEG C, insulation 3h, repeat above-mentioned normal pressure to boil and operate 2 times with low temperature insulation, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 120r/min, Homogeneous phase mixing, in power 200W, ultrasonication 10min is carried out when frequency 45KHz, simultaneously at strength of electric field 45kV/cm, burst length 600 �� s, pulsed electric field process is carried out when pulse-repetition 600Hz, last in 0.2 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

The hoof body lotion quality guaranteed period prepared through aforesaid method is 46 months.

Embodiment 7 hoof body lotion storage stability test of the present invention

Hoof body lotion is as a kind of liquid preparation prepared primarily of extracts of Chinese herbal medicine, with reference to the stability criterion of liquid preparation of Chinese medicine, clarity is defined as the quality stability index of hoof body lotion, it not only directly reflects the visual appearance of product, also reflect product inner quality and stability, it is the hoof the most important quality control index of body lotion.

Clarity characterizing method: clear 90-100 divides; Micro-turbid 80-89 divides; Muddy 70-79 divides; More muddy 60-69 divides; Serious muddy 50-59 divides; Precipitate less than 49 points (deposit seeds is more big, quantity is more many, and mark is more low).

Hoof body lotion prepared by the embodiment of the present invention 2 and commercially available of the same type, same specification, with the hoof body lotion of date manufactured in room temperature, ventilation, lucifuge condition storage, observe hoof body lotion clarity, every 6 months observed and recorded results once, the results are shown in Table 1:

Table 1: storage phase hoof body lotion clarity detected result

Time (moon)	6	12	18	24	30	36	42	48	54	Always divide equally
											Commercially available	95	90	85	78	73	65	55	45	30	68.44
The present invention	100	98	95	93	93	92	90	82	81	91.56

Above result shows: it is better that hoof body lotion of the present invention compares visual appearance with commercially available hoof body lotion, clarity height, along with the prolongation of time, hoof body lotion liquid phase is relatively unstable, according to use and the drug effect standard of hoof body lotion, the hoof body lotion quality guaranteed period of the present invention can reach 42-54 month, extends 18-30 month than commercially available (24 months), also illustrate that inner quality of the present invention is better simultaneously, drug effect safety, stable.

It should be understood that hoof body lotion prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 8 hoof body lotion of the present invention prevention Milk cow hoof disease epidemic prevention test

1 materials and methods

1.1, test materials

1.1.1, the embodiment of the present invention 2 prepare hoof body lotion and commercially available hoof body lotion

1.1.2, milk cow is tested: test carries out in a certain diary farm of Beijing Miyun, and kind is china holstein cows 2 tire milk cow above perinatal period, totally 46, all without hoof disease, wherein 16,2 tire, 3 12, tires, 4 10, tires, 58, tires. The pair principle that test ox presses parity, milk yield is identical or close in earlier stage, is divided into experimental group and control group, often organizes 23.

1.2, daily ration composition is tested: test group and control group adopt same feeding and management method. Daily ration consists of fine fodder (corn 52.7%, wheat bran 8%, dregs of beans 7%, cotton dregs 9%, peanut meal 9%, corn DDGS 8%, Preblend 7115A6%), and coarse fodder is corn silage, sheep's hay, the beans stalks of rice, wheat, etc., clover etc.

1.3, test method: test group and control group be hoof bath epidemic prevention hoof disease respectively, test group uses the hoof body lotion of the embodiment of the present invention 2 preparation, control group uses commercially available hoof body lotion, using method: prevent requirement according to hoof disease, thoroughly clean cow hoof portion, guarantee that hoof and toe gap do not have ight soil to remain, make it clean, hoof body lotion is configured to the liquid that mass percent concentration is 5%, then add in special milk cow hoof bath, liquid level is 15cm at least, milk cow hoof soaks 25min in special hoof bath and namely completes a hoof bath, hoof body lotion is changed when same hoof bath hoof bathes 200 times. every day is sooner or later each continuous 3 days is once a preventative epidemic prevention cycle, quarterly an epidemic prevention cycle, 6) observing between trial period, record test group and the various hoof disease generation situation of control group milk cow, add up hoof disease incidence the end of the year, result is such as table 2, the fine fodder 4-8kg of giving milk of all autogamys in field of feeding for examination milk cow, adjusts according to raising stage and milk yield, corn silage quantitative feeding 20kg/ days, roughage (sheep's hay, the beans stalks of rice, wheat, etc., clover) free choice feeding.

1.4, test period: trial period terminated from June 11,2015 on the 12nd June in 2014, lasted a year.

Table 2: Milk cow hoof disease epidemic prevention result

Above result shows: hoof body lotion of the present invention can effectively prevent the generation of the various body disease of milk cow, especially ulcer at the bottom of foot rot, hoof, wart dermatitis and other hoof disease is had effect of preventing epidemic preferably, and compared with commercially available hoof body lotion, its hoof disease incidence reduces by 39.14%. Also illustrate that hoof body lotion of the present invention has good perviousness, cohesiveness, high efficiency simultaneously.

The effect test of embodiment 9 hoof body lotion of the present invention when treating Milk cow hoof disease

For verifying the clinical efficacy of hoof body lotion of the present invention, in October ,-2015 in June, 2014 in three milk cattle cultivating fields in Hebei province and Inner Mongolia Autonomous Region, select 320 example Milk cow hoof diseases altogether, wherein wart dermatitis 100 example, hyperplasia 60 example between toe, ulcer 70 example at the bottom of hoof, foot rot 50 example, other type hoof disease 40 example, two groups it are divided at random: test group and control group according to hoof disease type, hoof body lotion prepared by the embodiment of the present invention 2 and commercial hoof body lotion is not used to treat through plant animal doctor and Xiu hoof work point, test group and control group are except medication (hoof body lotion) is different, other is raised and management mode, daily ration, methods for the treatment of, feed environment, efficacy evaluations etc. are all identical.

Case selection standard: have symptom of obviously walking lamely, happiness is lain prone sleeping, and difficulty of standing or stand up time are shorter; The suffering limb unreal or each limb hoof of heavy burden alternately bears a heavy burden; Palpation suffering limb hoof temperature rise, pain sensitivity during percussion. Sick ox performance spirit is depressed, and poor appetite, milk yield declines, and body temperature raises sometimes, can reach more than 40 DEG C. Such as aggravation, indivedual ill milk cow shows some constitutional symptoms, and severe one trouble portion is red and swollen, does not rise sleepingly.

Using method: treat requirement according to hoof disease, thoroughly clean cow hoof portion, guarantee that hoof and toe gap do not have ight soil to remain, make it clean, hoof body lotion being configured to the liquid that mass percent concentration is 7%, then adds in special milk cow hoof bath, liquid level is 15cm at least, milk cow hoof soaks 25min in special hoof bath and namely completes a hoof bath, changes hoof body lotion when same hoof bath hoof bathes 200 times. Every day, sooner or later respectively once it 4 days was a treatment cycle that continuous hoof is bathed, and test group and control group respectively carry out a treatment cycle.

Curative effect determinate standard: observe sick ox symptom, determine curative effect according to criterion for the 7th day, 14 days after hoof bath. Suffering from ox limping to disappear, pain disappears, and swelling is disappeared, and wound healing is for curing; Pain obviously alleviates, and swelling scope reduces, walk lamely alleviate or not obvious be effective; Pain does not disappear, and swelling, without disappearing, is walked lamely without disappearing, and what have dyskinesia is invalid. Total effectively head number equals recovery from illness and effective head number summation.

The result for the treatment of is carried out statistics and analysis, specifically as shown in Table 3, 4:

Table 3: hoof bathes 7 days treatment result

Table 4: hoof bathes 14 days treatment result

Above result shows: various Milk cow hoof disease can fast, effectively be treated by hoof body lotion of the present invention, cow hoof disease being had antibacterial anti-infection, detoxifies and restrain, remove necrosis and promote granulation and promote the effect of wound healing, perviousness is strong compared with commercial hoof body lotion, drug effect height, cohesiveness are strong. when hoof bathes 7 days, its curative ratio has reached 65.7-80%, has always had efficiency to reach 88.6-94%, 49.7-66.7% higher than control group (13.3-16%) and (55-80%) and 8.6-39% respectively, when hoof bathes 14 days, its curative ratio has reached 90-96%, has always had efficiency to reach 96.7-100%, 14-36% higher than control group (60-76%) and (70-86%) 10.7-30% respectively, reach same therapeutic efficiency, treatment time at least shortens 7 days, particularly to wart dermatitis, the hoof disease result for the treatment of of foot rot and other type is best, illustrate simultaneously, therapeutic process is not when using antibiotics assisting therapy, hoof body lotion treatment time of the present invention is short, efficiency height, not only reduce milk cattle cultivating cost, add fanning economics, and improve the food safety of milk cow deep processed product, also protect breeding environment simultaneously, stop because using microbiotic, the potential safety hazard that chemistry and toxic substance produce, for application hoof body lotion treatment Milk cow hoof disease opens a new green shortcut.

Embodiment 10 hoof of the present invention body lotion is on the impact of milk cow production performance, reproductive performance and anti-disease

1 materials and methods

1.1, test materials

1.1.1, product of the present invention

1.1.2, milk cow is tested: embodiment 9 experimental group and control group are through treating the milk cow of hoof disease.

1.3, test method: according to milk cow breeding record, test group is except taking same method to feed with control group, the fine fodder 4-8kg of giving milk of all autogamys in field of feeding for examination milk cow, adjust according to raising stage and milk yield, corn silage quantitative feeding 20kg/ days, roughage (sheep's hay, the beans stalks of rice, wheat, etc., clover) free choice feeding.

1.4, production, metabolism, reproductive performance record: the milk yield measuring and recording every cow head in during 120 days postpartum; Observe, evaluate milk cow body condition; Detection reproductive performance; During 120 days postpartum, observed and recorded is participated in the experiment the rutting situation of milk cow, and whether gestation is as the criterion with examination per rectum in latter 60 days of breeding.

1.5, disease surveillance and record: to milk cow incidence diseases such as () displacement of mastitis, endometritis, ketoacidosis, cud, intractable apositia, lochiopyra and retention of placenta detection, the same day was monitored retention of placenta incidence in childbirth, and definition afterbirth not discharge person in 12-24 hour is retention of placenta. Adopt Ketone benzene detection ketosis of dairy cows, adopt CMT-Test reagent detection mastitis.

1.6, raw data is processed by statistical study EXCEL software, then carries out variance analysis by SAS [11] method.

2. test-results

2.1 hoof body lotions are on the impact of milk cow body condition:

Table 5 milk cow 5 part body condition standards of grading

Score value	Vertebra portion	Rib portion	Buttocks both sides	Tail root both sides	Hipbone, ischium joint
						1	Very outstanding	Root root is visible	Serious sagging	Crypts is very dark	Very outstanding
2	Obviously outstanding	Most visible	Obviously it sink	Crypts is obvious	Obviously outstanding
						3	Outstanding a little	Minority is visible	Sagging a little	Crypts is slightly aobvious	Outstanding a little
4	Straight	Lose completely	Straight	Crypts does not show	Do not show outstanding
						5	Plentiful	Plentiful	Plentiful	Plentiful	Plentiful

Table 6 milk cow body condition visual inspection appraisal result

Above result shows: the trend that milk cow body condition is marked and is significantly improved by test group hoof body lotion, milk cow can be improved to the digestion of nutritive substance, absorption and metabolism, be conducive to promoting and improving the synthesis of the framework material such as albumen, fat in body, obviously can improve the body condition of milk cow.

2.2 hoof body lotions are on the impact of milk production of cow

Table 6120 day milk production of cow

	Head number (head)	Give milk number of days (my god)	Total milk yield (kg)	Average milk production (kg/ days. head)
					Control group	160	120	736704	38.37��5.16
Test group	160	120	874560	45.75��5.92
					Process difference^b				7.38

Above result shows: test group is compared with control group, and head day increases milk yield 7.38 kilograms, it is to increase 19.23%, t checks significant difference (P < 0.05), and hoof body lotion can significantly improve the milk yield of lactating cows.

2.3 hoof body lotions are on the impact of immunity of cow

Table 7 cow serum perinatal period immunoglobulin content detected result

In cow serum, the content of Immunoglobulin IgA, IgM, IgG is the important indicator judging milk cow humoral immunity level. As seen from the experiment, cow birth stress cause serum immune globulin IgA, IgM, IgG content sharply to decline, and humoral immune function is suppressed. Test group hoof body lotion can increase before cow birth 1 week, the childbirth same day and point 1 week puerperium serum IgA, IgM, IgG content, enhancing humoral immune function.

2.4 hoof body lotions are on the impact of cow reproduction performance perinatal period

Table 8 cow reproduction perinatal period performance test results

Test-results shows, compared with control group, test group milk cow perinatal period can shorten nearly 15 days of the time that first time in milk cow postpartum oestruses greatly, it is to increase the conception rate 20.5% of feelings in postpartum first phase milk cow, increase the ratio of oestrusing 18.5% of Postpartum Cows colony, effectively improve the reproductive performance of milk cow.

2.5 hoof body lotions are on the impact that perinatal period, postpartum disease of cow occurred

Table 9 postpartum disease of cow perinatal period detected result

Above result shows: test group is compared with control group, in prevention postpartum disease of cow, can significantly reduce the sickness rate of various disease, on average reduce 10-28.7%, t checks significant difference (P < 0.05), hoof body lotion can significantly reduce the incidence of the diseases such as milk cow mastitis in postpartum, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and retention of placenta, improves the healthy state of milk cow.

To sum up, it may also be useful to hoof body lotion of the present invention can significantly improve milk cow physique, immunizing power production performance, reproductive performance and the various disease of prevention, what effectively prevent from bringing because of Milk cow hoof disease directly and indirectly cultivates harm,

Claims

1. a biological environmental production milk cow hoof body lotion, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 60-80 part, Aloe extract 40-60 part, plant milk extract 20-40 part, modified dietary fiber 6-12 part, trehalose 5-11 part, egg white 3-9 part, natural antioxidants 5-11 part, M-Zyme 0.5-1.5 part, at 0.1-1 part;

Described plant milk extract be taking can change milk cow hoof stratum corneum structure, promote component rapid osmotic to deep skin and the natural phant absorbed prepare as raw material.

2. biological environmental production milk cow hoof body lotion as claimed in claim 1, it is characterized in that, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 65-75 part, Aloe extract 45-55 part, plant milk extract 25-35 part, modified dietary fiber 8-10 part, trehalose 7-9 part, egg white 5-7 part, natural antioxidants 7-9 part, M-Zyme 0.8-1.2 part, at 0.4 0.6 parts.

3. biological environmental production milk cow hoof body lotion as claimed in claim 1, it is characterized in that, prepared by the raw material primarily of following weight part number: extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant milk extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts, egg white, natural antioxidants 8 parts, M-Zyme 1.0 parts, at 0.5 part.

4. the biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-3, it is characterised in that, the preparation method of described extracts of Chinese herbal medicine, comprise the steps: to count by weight, take frankincense 20-25 part, dragon's blood 18-20 part, Radix Astragali 13-16 part, myrrh 12-15 part, keel 12-15 part, Radix Achyranthis Bidentatae 8-12 part, Wu Chia Pee 7-9 part, Plant Soot 4-6 part, purslane 4-6 part, pseudo-ginseng 2-4 part, Root-bark of Densefruit Pittany 1-3 part, Cortex Meliae 1-3 part; Put in the Ultrasonic Cleaners filling 0.1-0.3% sodium hydrogen carbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixture, control temperature 70-90 DEG C keeps 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 by lactic acid adjust ph, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic-assisted extraction; Then the prozyme adding mixture gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 by lactic acid adjust ph, enzymolysis 2-4h, finally add the mixture of mixture 0.5-3 times of w ethanol and propyl alcohol, the mass ratio of ethanol and propyl alcohol mixing is 1:1-3, control temperature keeps 3-4h to 60-78 DEG C, filters, obtains the first filtrate; Adding the water of filter residue 1-3 times of weight, control temperature 85-95 DEG C keeps 1-3h, is then cooled to 25-35 DEG C, filters, obtains the 2nd filtrate; First filtrate and the 2nd filtrate being merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtains extracts of Chinese herbal medicine;

5. the biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described Aloe extract, comprise the steps: fresh aloe, orange peel, Pericarpium Vitis viniferae, hawthorn pomace to be put into the Ultrasonic Cleaners that 1.2% sodium hydrogen carbonate solution is housed respectively and clean 5-10min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min; Pulverizing immediately, particle diameter is 0.1-1mm; Add crushed material quality 3-5 deionized water doubly, mix, be 2-3 by citric acid adjust ph, at strength of electric field 35-45kV/cm under room temperature, in burst length 400-600 �� s, carrying out pulsed electric field process when pulse-repetition 200-400Hz, then normal pressure boils 30-50min, Bag filter, filter residue boils 40-60min, Bag filter, merging filtrate with the filtrate normal pressure of its quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

6. the biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described plant milk extract, comprise the steps: Radix Angelicae Sinensis, Leaf of Chinese Wampee, cloves, the red sage root, peppermint is cleaned respectively, drain, 10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio, add the wetting 3-8h of lactic acid that mixture quality 0.3-0.5 pH value doubly is 4.5-5.5, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing bed thickness 3-5cm, crushed material particle diameter 0.5-1mm, then crushed material quality 4-6 water doubly is added, it is 3.5-5.5 by lactic acid adjust ph, at strength of electric field 25-35kV/cm under room temperature, burst length 300-500 �� s, pulsed electric field process 20-30min is carried out when pulse-repetition 200-300Hz, then carry out microwave exposure when power 150-300W in room temperature and extract 15-20min, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic-assisted extraction, add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate decompression concentrates to solid content is that namely 8-10% obtains plant milk extract,

7. the biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, wheat fiber, apple fibre, oat fibre 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add its quality 3-7 water doubly, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at strength of electric field 20-40kV/cm, in burst length 300-500 �� s, when pulse-repetition 200-400Hz, carry out pulsed electric field process 10-15min; It is 4.5-6.5 by lactic acid adjust ph, adds the biological enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min; Enzymolysis solution filters, and it is that namely 5-8% obtains modified dietary fiber that filtrate decompression concentrates to solid content;

8. the biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-3, it is characterised in that, described natural antioxidants contains any one or a few in tea-polyphenol, grape pip procyanidin, rosemary extract, Radix Glycyrrhizae extract, apricot leaf extract and Vc.

9. the preparation method of biological environmental production milk cow hoof body lotion as described in as arbitrary in claim 1-8, it is characterized in that, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant milk extract Homogeneous phase mixing, put into colloidal mill, regulate the gap between stators and rotators, outlet mixture particle diameter is made to reach 100-200 ��m, then by high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 100-120MPa, make clarifixator outlet material particle diameter be 100-150nm, obtain nanoparticle mixed solution, egg white is put into stirrer simultaneously and stirs 12-20min in rotating speed 600-720r/min, until egg white all becomes foam obtains egg white foam, then intensification nanoparticle mixed solution is to 65-75 DEG C, insulation, add egg white foam while stirring successively, modified dietary fiber, trehalose, mixing speed 130-150r/min, fully dissolve 20-30min, stop stirring, heat up, normal pressure boils 5-10min, naturally room temperature it is down to, continue to be cooled to-1.5-0.5 DEG C, insulation 1-3h, repeat above-mentioned normal pressure to boil and low temperature insulation operation 2-4 time, natural antioxidants is added while stirring successively under room temperature, at and M-Zyme, mixing speed 100-120r/min, Homogeneous phase mixing, in power 200-400W, ultrasonication 10-30min is carried out when frequency 35-45KHz, simultaneously at strength of electric field 35-45kV/cm, burst length 600-800 �� s, pulsed electric field process is carried out when pulse-repetition 400-600Hz, last in 0.2-0.4 ��m of micro-pore-film filtration, remove throw out, namely filtrate sterile filling obtains biological environmental production milk cow hoof body lotion.

10. the preparation method of biological environmental production milk cow hoof body lotion as claimed in claim 9, it is characterised in that, the quality group of described natural antioxidants becomes: grape pip procyanidin: tea-polyphenol: rosemary extract=4-6:2-4:1-3.