CN105748387A

CN105748387A - Dairy cow hoof bath foam without antibiotics and preparation method thereof

Info

Publication number: CN105748387A
Application number: CN201610101270.0A
Authority: CN
Inventors: 邵素英
Original assignee: Yiwu Juhui Biotechnology Co Ltd
Current assignee: Yiwu Juhui Biotechnology Co Ltd
Priority date: 2016-02-24
Filing date: 2016-02-24
Publication date: 2016-07-13

Abstract

The invention discloses dairy cow hoof bath foam without antibiotics. Chinese herbal medicine extracts capable of effectively killing and inhibiting dairy cow hoof disease infectious pathogenic bacteria, promoting hoof wound healing, effectively preventing and curing various hoof diseases, and improving dairy cow autoimmunity and premunition, and aloe extracts with strong cohesiveness capable of promoting medicated permeation and effectively repairing cuticula are adopted as main raw materials; plant extracts capable of changing dairy cow hoof cuticula structures and promoting the effective components such as Chinese herbal medicine and aloes to quickly penetrate into skin deep layer to absorb are added; ultrasonic dispersion, dissolution, high pressure homogenization and cooling-heating treatment technologies are combined organically, not only are modified dietary fibers with efficient bonding forces compounded scientifically, but also trehalose, albumen, natural antioxidant and the like capable of protecting effective constituents of components against oxidization and improving the physical and chemical stability of hoof care mist spray are added, and finally, the hoof bath foam with strong stability, safety, high efficiency, and no any antibiotics and toxic chemicals is obtained.

Description

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion and preparation method thereof

Technical field

The present invention relates to milch cow care of feed product, be specifically related to a kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion and preparation method thereof.

Background technology

In milk cattle cultivating, hoof disease, mastitis and Reproduction Disorder are cause milch cow to be forced to eliminate three big diseases, the limping of milch cow 90% causes due to hoof disease, therefore, hoof disease is the regular incidence of milch cow, and its cause of disease is mainly in daily ration calcium, phosphorus is uneven or trace element deficiency, cause cutin hoof to loosen, plus colony house bad environments, hoof often by fecaluria, mud dipping, hoof cutin softening and fall ill.It addition, the hard thing such as stone, iron filings, vegetation shred, fragments of glass, all can stab hoof soft tissue and cause infection inflammation.After milch cow suffers from hoof disease, appetite reduces, and quantity of motion reduces, and happiness is lain prone sleeping, limping, pain, occurs pain having hotness during palpation during percussion hoof.After expanding wound with poring iron, aperture at the bottom of hoof or namely have dirty black smelly water to flow out in hole, it can be seen that have ulcer between toe, cover cacodorous sphacelus.Severe one coronet is red and swollen, and pain substantially, can not stand, milk yield degradation, and reproductive capacity reduces, and finally eliminates by sick cattle.Along with developing rapidly of China milk industry, from abroad having introduced a large amount of high-quality milch cow, frozen semen and embryo, but these high yield cows are likely to less adapt to domestic feeding environment and diet nutrient, cause hoof disease sickness rate to have the trend of rising, cause huge economic loss to China's milk cow production.According to statistics, in China, some areas dairy cow farm hoof disease sickness rate is about 50%, and ill rear cow feeding amount reduces by 20%, milk yield reduces by 20%, eliminating ox head number because of hoof disease and account for the 50% of total superseded cattle, medical expense increases by 30%, and the economic loss caused because of hoof disease accounts for the 10% of annual profit.Therefore, preventing and treating Milk cow hoof disease, it is possible to reduce cow culling rate, significantly improve the economic benefit of China's milk cattle cultivating.

Hoof disease is a very ambiguous and general idea, is summed up it and actually includes a lot of disease, common are between ulcer at the bottom of hoof, hoof toe dermatitis, wart dermatitis etc. between skin proliferation, toe.Produce the main infectious cause of disease of the common cause of disease of above-mentioned hoof disease, metabolic disorder, environmental factors and hereditary factors, the measure correspondingly taked mainly carries out care of feed, foot paring and clinical treatment, be hoof disease prevent or adopt to treat in any way all can relate to a series of care of feed medicine, such as care of feed cream, care of feed liquid, hoof body lotion and care of feed spraying etc., in these medicines, antibiotic medicine occupies significant proportion, but use antibiotic product milch cow and the mankind can be produced significant impact in a large number, Drug resistance in milch cow and mankind's body is caused to strengthen, greatly compromise milch cow, the physical health of poultry feeders and epidemic prevention worker, breeding environment can be caused to pollute simultaneously, also can strengthen the safety risks of food (milk and beef).

Hoof body lotion applies to improve a kind of care of feed product of milch cow colony hoof health care, recovery stage after colony's epidemic prevention or foot paring and clinical treatment is entered when milch cow, occur according to hoof disease or treatment degree, thoroughly clean Ungula Bovis seu Bubali portion, guarantee that hoof and interdigit do not have feces to remain, so as to it is clean, hoof body lotion is configured to certain density medicinal liquid, it is subsequently adding in special milk Ungula Bovis seu Bubali bath, liquid level is 15cm at least, milch cow pass by special hoof bath namely complete hoof bath, to prevent hoof disease from occurring and nursing hoof health, therefore, hoof bath is the of paramount importance measure preventing Milk cow hoof disease, hoof body lotion is to prevent the indispensable care of feed product of Milk cow hoof disease.

Chinese herbal medicine specimen is ruled together, than Western medicine (as: tradition hoof body lotion is copper-bath or the 3-5% formalin solution of 5-10%) cured the symptoms, not the disease a lot, hoof disease can be effected a radical cure, and side effect is little, pure natural, it is easy to degraded, environmentally friendly, at present, relevant Chinese medicine care of feed product has been widely used for milk cattle cultivating.But, Chinese medicine kind is many, complicated component, poor as its pharmacodynamic stability of Liquid traditional Chinese medicine preparation, and based on the breeding environment of milch cow, feeding pattern, the feature of bringing out of hoof disease and prevent and remedy measures, the therapeutic effect of bound drug, the disease resistance of hoof body lotion, adhesiveness and permeability etc. are still the emphasis of concern.

Chinese patent CN104825486A discloses a kind of composition of medicine for treating claw ill, and including ethylenediamine dihydroiodide and copper sulfate, the mass mixing ratio of ethylenediamine dihydroiodide and copper sulfate is 1:2～2:3.Ethylenediamine dihydroiodide can react with copper sulfate, discharge free iodine, the atomic iodine of free state has superpower Oxidation, it is possible to makes protein denaturation and destroys the membrane structure of pathogen, thus reaching the purpose that prevention is infected and treated claw ill.Compositions Main Function in patent disclosed above is the surface oxidation sterilization utilizing the atomic iodine of strong oxidation to carry out disease hoof, only carry out disinfecting of surface, can not effect a radical cure, and intractable fungus effect is very micro-, osmosis is weak, and therapeutic effect is undesirable；Also with antibiotics use, to have side effects, and compositions be susceptible to chemical reaction simultaneously, not easily circulation, storage and use.

Chinese patent CN102552339A discloses a kind of nanoemulsion medicine for treating foot rot, this nano-emulsion particle diameter is between 1～100nm, being made up of the raw material of following component: pine tar 0.1%～10.0%, cinnamic aldehyde 0.1%～6.0%, carvacrol 0.1%～6.0%, Oleum Camphora 0.1%～5.0%, clobetasol propionate 0.01%～0.1%, surfactant 18.0%～35.0%, cosurfactant 0～7.0%, surplus are distilled water, the mass percent sum of mentioned component is 100%.This nano-emulsion has dissolving cutin, antipruritic, antiinflammatory, convergence, partly sterilised's anticorrosion and promotes the effects such as absorption, is mainly used in treatment animal foot rot.Invention increases the dissolubility of medicine, improve medicine stability and bioavailability, decrease drug dose, skin permeation rate is higher than the general formulations such as unguentum, and with low cost, has wide market prospect in field of veterinary.The medicament categories that newborn medicine disclosed above is composite is less, effect is single, it is impossible to the hoof disease of complete treatment milch cow, and containing glucocorticoids medicine clobetasol propionate and the bigger pine tar of toxicity, Camphora wet goods, easily penetrate into from site of injury, produce certain side effect.

Chinese patent CN102100750A discloses a kind of Chinese medicine lotion and preparation method thereof, belongs to herbal medicine product preparation method technical field.Ingredient by by quality is: Indigo Naturalis 0.5-2 part；Realgar 1-3 part；Dried Alumen 1-3 part；Rhizoma Coptidis 0.5-2 part；Radix Et Rhizoma Rhei 1-3 part；Borax(calcined) 0.5-2 part；Catechu 0.5-2 part；Herba Menthae 1-3 part is constituted, each for medicine except Herba Menthae component is added the cold water soak 30 minutes of 10 times of medical material amounts, decoct 20 minutes, adding Herba Menthae to decoct 10 minutes again, filter, medicinal residues add the soak by water 30 minutes of 8 times of medical material amounts again, filter, merge twice filtrate, be evaporated to every 1ml medicinal liquid concentrated medicament containing crude drug in whole 2g, add the mature vinegar of equivalent after cooling and get final product.This preparation method production technology is simple, and equipment investment is few, and product price is cheap, is suitable for industrialized production.Patent Chinese medicine disclosed above adopts traditional decocting medicine, utilization rate of active components is low, loss is big, is sprayed to disease hoof surface adhesiveness poor, it is easy to come off, osmosis is poor, the focus on below top layer can not be treated, and containing toxic bigger Realgar, extremely toxic substance arsenicum can be produced under decoction process and light conditions, use and storage process exists potential safety hazard, poor stability.

Chinese patent CN104258319A discloses a kind of medicinal herb spray treating cattle footrot, Fructus Rosae Laevigatae, Caulis Spatholobi, Herba Violae japonicae, Folium Artemisiae Argyi, Colophonium, Galla Chinensis, Rhizoma Zingiberis load body after extracting and prepare.To sick cattle, the product of the present invention being carried out spray delivery, 2 times on the one, is used in conjunction 5～7, can effectively control cattle footrot, easy to use, cost is low, noresidue, and biological safety is high.Patent Chinese medicine kind disclosed above is few, the foot rot in Milk cow hoof disease can only be treated, can not preventing comprehensively and treat various hoof disease, and adopt traditional decocting medicine, utilization rate of active components is low, loss is big, it is sprayed to disease hoof surface adhesiveness poor, it is easy to coming off, osmosis is poor, the focus on below top layer can not be treated, be also added with the chemical substance such as preservative, stabilizer as carrier simultaneously.

Chinese patent CN102846489B discloses a kind of Polyhaxemethylenguanidine Hydrochloride iodine dip and preparation method thereof, its components in mass portion number includes: iodine 0.2-2 part, potassium iodide 0.5-5 part, potassium iodate 0.1-1 part, sodium tripolyphosphate 0.1-1 part, Polyhaxemethylenguanidine Hydrochloride 5-50 part, detergent 1-20 part, skin protectant 0.5-5 part, citric acid 0.05-1 part, water 15-92.55 part: its preparation includes: Polyhaxemethylenguanidine Hydrochloride detergent and skin protectant are added to the water respectively, in 45-70 DEG C of stirring to mix homogeneously, it is subsequently adding iodine, potassium iodide, potassium iodate, stir at 45-70 DEG C, it is made fully to dissolve；Adding sodium tripolyphosphate and citric acid, at 45-70 DEG C, stirring to each component is completely dissolved, and to obtain final product.The available iodine content of the dip of the present invention is big, fungicidal effectiveness is high, good stability, is suitable for pasture and uses；The preparation method of the present invention is simple to operate, is produced on a large scale.Body lotion disclosed above is mainly used in sterilization, it is impossible to well prevent Milk cow hoof disease, and containing more chemical substance, contaminated environment.

Chinese patent CN104257684A discloses a kind of for nipple dipping liniment preventing bovine mastitis and preparation method thereof.Nipple dipping liniment therein is prepared from by more than ten raw materials such as film former.Advantages of the present invention has three aspects, and first is addition film former in dip, forms barrier between nipple and environment.Second is addition skin protectant in dip, protects skin of nipple.3rd is sterilization, film forming and skin protection are organically combined, and increases the function of nipple dipping disinfectant, omnibearing protection nipple, more effectively prevents the generation of bovine mastitis.Dip disclosed above mainly prevents bovine mastitis, it is impossible to prevent Milk cow hoof disease.

To sum up, prepare a kind of can prevent comprehensively milch cow all kinds hoof disease, stability, adhesiveness and permeability hoof body lotion strong, safe and efficient, without any antibiotic and chemical toxic substances be numerous raisers in the urgent need to, be also responsibility and the pursuit of those skilled in the art.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing milk Ungula Bovis seu Bubali body lotion, effectively to kill and to suppress Milk cow hoof disease infectivity pathogen and to promote hoof wound healing, it is possible to effectively prevent and treat multiple hoof disease, improve the extracts of Chinese herbal medicine of milch cow autoimmunity and premunition and have promotion drug osmotic, effectively repair horny layer, the Aloe extract of strong cohesive property is primary raw material；Science compound highly effective bonding modified dietary fiber, milch cow hoof horny layer structure the plant extract promoting the effective ingredient rapid osmotic such as Chinese herbal medicine to absorb can be changed to deep skin；Science is composite simultaneously has anti-freezing, high temperature resistance high temperature, can ensure that in hoof body lotion the trehalose of bioactive ingredients liquid stable and component effective ingredient can be protected from oxidation of resist drying performance, improve Ovum Gallus domesticus album and the Natural antioxidant of hoof body lotion physical and chemical stability, and with the addition of the keratinase promoting that hoof body lotion bonds and permeates, at etc., final prepared a kind of stability is strong, can complete treatment milch cow all kinds hoof disease, it is firmly bonded, permeability is strong, safe and efficient, hoof body lotion without any antibiotic and chemical toxic substances, reduce milk cattle cultivating cost, improve fanning economics, add the safety of milch cow deep-processed food, protect environment.

In order to achieve the above object, the present invention adopts the following technical scheme that

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 60-80 part, Aloe extract 40-60 part, plant extract 20-40 part, modified dietary fiber 6-12 part, trehalose 5-11 part, Ovum Gallus domesticus album 3-9 part, Natural antioxidant 1-4 part, keratinase 1-4 part, at 1-4 part；

Preferably, a kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 65-75 part, Aloe extract 45-55 part, plant extract 25-35 part, modified dietary fiber 8-10 part, trehalose 7-9 part, Ovum Gallus domesticus album 5-7 part, Natural antioxidant 2-3 part, keratinase 2-3 part, at 2-3 part；

It is highly preferred that a kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, mainly prepared by the raw material of following parts by weight:

Extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts of Ovum Gallus domesticus album, the Natural antioxidant 2.5 parts, keratinase 2.5 parts, at 2.5 parts；

Further, described extracts of Chinese herbal medicine is effectively to kill and suppress Milk cow hoof disease infectivity pathogen, and there is promoting blood circulation and stopping pain, detumescence and promoting granulation, dampness hemostasis, parasite killing removing toxic substances, removing dampness, sore, spasmolytic, heat of dispelling, it is possible to the Chinese medicine effectively preventing and treating multiple hoof disease, raising milch cow autoimmunity and premunition is prepared by raw material；

Preferably, the preparation method of described extracts of Chinese herbal medicine, comprise the steps: to count by weight, weigh Olibanum 20-25 part, Sanguis Draxonis 18-20 part, Radix Astragali 13-16 part, Myrrha 12-15 part, Os Draconis 12-15 part, Radix Achyranthis Bidentatae 8-12 part, Cortex Acanthopancis 7-9 part, Pulvis Fumi Carbonisatus 4-6 part, Herba Portulacae 4-6 part, Radix Notoginseng 2-4 part, Cortex Dictamni 1-3 part, Cortex Meliae 1-3 part；Put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 with breast acid for adjusting pH value, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 with breast acid for adjusting pH value, enzymolysis 2-4h, finally add the mixture of 0.5-3 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:1-3, control temperature and keep 3-4h to 60-78 DEG C, filter, obtain the first filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, be then cooled to 25-35 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

Described compound enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 5-7:2-4:2-4:1-3:1-3 Homogeneous phase mixing in mass ratio.

Further, described Aloe extract is with Aloe for primary raw material, the waste residue such as peel or Fructus Crataegi, Radix Betae, Fructus Mali pumilae such as Fructus Citri grandis that the composite pectin content of science is higher, Fructus Citri Limoniae, orange, mandarin orange, Fructus Vitis viniferae extracts through ultrasonic cleaning, high-voltage pulse, hot water extraction and prepared；

Preferably, the preparation method of described Aloe extract, comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, being cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Adding the deionized water of ground product quality 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, carries out high voltage pulse electric field processing when pulse frequency 200-400Hz, then normal pressure boils 30-50min, Bag filter, filtering residue boils 40-60min, Bag filter, merging filtrate with its filtrate normal pressure of quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

Further, described plant extract is can change milch cow hoof horny layer structure, to promote that hoof body lotion component rapid osmotic is prepared for raw material to deep skin the natural plants that absorbs.Such as: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae etc.；

nullPreferably，The preparation method of described plant extract，Comprise the steps: Radix Angelicae Sinensis、Folium clausenae lansii、Flos Caryophylli、Radix Salviae Miltiorrhizae、Herba Menthae is respectively washed、Drain，10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 4.5-5.5 of mixed material quality 0.3-0.5 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-1mm,It is subsequently added into the water of ground product quality 4-6 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 8-10% obtains plant extract；

Described mixed enzyme is cellulase, hemicellulase, pectase, tannase, protease 3-5:3-5:2-4:1-3:0.3-0.5 Homogeneous phase mixing in mass ratio.

Further, described modified dietary fiber is the impact with strong retentiveness, dilatancy, thickening property, adsorptivity and acid and alkali, alkali, salt that dietary fiber is processed through physics, chemical or biological method and obtained, and significantly improves caking property and the physical and chemical stability of antibiotic-free milk Ungula Bovis seu Bubali body lotion；

Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber；

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min；Enzymolysis solution filters, and filtrate reduced in volume to solid content is that namely 5-8% obtains modified dietary fiber；

Described enzyme is cellulase, glucanase, xylanase, pectase 5-7:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.

Further, the described Natural antioxidant contains any one or a few in tea polyphenols, grape pip procyanidin, Herba Rosmarini Officinalis extract, Radix Glycyrrhizae extract, Folium Pruni extract and Vc；

Preferably, the quality group of the described Natural antioxidant becomes: grape pip procyanidin: tea polyphenols: Herba Rosmarini Officinalis extract=4-6:2-4:1-3.

The preparation method that another object of the present invention is to provide above-mentioned antibiotic-free milk Ungula Bovis seu Bubali body lotion, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100-200 μm, then pass through high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 100-120MPa, making homogenizer outlet material particle diameter is 100-150nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 12-20min in rotating speed 600-720r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 65-75 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 130-150r/min,Fully dissolve 20-30min,Stop stirring，Heat up，Normal pressure boils 5-10min，Naturally room temperature it is down to，Continue to be cooled to-1.5-0.5 DEG C，Insulation 1-3h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 2-4 time，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 100-120r/min,Homogeneous phase mixing，In power 200-400W,Ultrasonic Treatment 10-30min is carried out when frequency 35-45KHz,Simultaneously at electric field intensity 35-45kV/cm，Burst length 600-800 μ s,High voltage pulse electric field processing is carried out when pulse frequency 400-600Hz，Last in 0.2-0.4 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

The using method of antibiotic-free milk Ungula Bovis seu Bubali body lotion of the present invention: prevent according to hoof disease or treat requirement, thoroughly clean Ungula Bovis seu Bubali portion, guarantee that hoof and interdigit do not have feces to remain, so as to it is clean, hoof body lotion being configured to the medicinal liquid that mass percent concentration is 4-8%, is subsequently adding in special milk Ungula Bovis seu Bubali bath, liquid level is 15cm at least, milch cow hoof soaks 20-30min in special hoof bath and namely completes a hoof bath, changes hoof body lotion when same hoof bath hoof bathes 200 times.

In the prevention cycle: liquor strength 4-6%, every day is sooner or later each is once an epidemic prevention cycle for three days on end, quarterly an epidemic prevention cycle；

Treatment cycle: liquor strength 6-8%, every day, sooner or later respectively once the bath of continuous hoof 4 days was a treatment cycle；

Beneficial effect:

nullThe present invention is effectively to kill and to suppress Milk cow hoof disease infectivity pathogen and to promote hoof wound healing，Can effectively prevent and treat multiple hoof disease、Improve the extracts of Chinese herbal medicine of milch cow autoimmunity and premunition and there is promotion drug osmotic、Effectively repair horny layer、The Aloe extract of strong cohesive property is primary raw material，Interpolation can change milch cow hoof horny layer structure and promote Chinese herbal medicine、The plant extract that the effective ingredient rapid osmotic such as Aloe absorb to deep skin，By ultrasonic disperse、Dissolving technology organically combines with high pressure homogenization technique，Further by material effective component granule nanorize，Prepare into more stable、It is easier to infiltration and the nanoparticle mixed solution absorbed，The not only modified dietary fiber of science compound highly effective bonding，And with the addition of and component effective ingredient can be protected from oxidation，Improve the trehalose of hoof body lotion physical and chemical stability、Ovum Gallus domesticus album and the Natural antioxidant，With the addition of the keratinase promoting that hoof body lotion bonds and permeates simultaneously、At，Final prepared a kind of stability is strong、Can complete treatment milch cow all kinds hoof disease、It is firmly bonded、Permeability is strong、Safe and efficient、Hoof body lotion without any antibiotic and chemical toxic substances，It is with natural plants for raw material，Adopt low temperature processing techniques，After cold-heat treatment，Hoof body lotion transparency、Stability is higher，The shelf-life extending hoof body lotion is 42-54 month，18-30 month is extended than commercially available (24 months)，Without any chemical toxic substances，Prevention and treatment Milk cow hoof disease process do not use any antibiotic，Reduce milk cattle cultivating cost，Improve fanning economics，Add the safety of milch cow deep-processed food，Protect environment.Experiment proves (embodiment 7-10): 1) to compare presentation quality with commercially available hoof body lotion better for hoof body lotion of the present invention, clarity is high, prolongation over time, hoof body lotion liquid phase is relatively unstable, according to use and the drug effect standard of hoof body lotion, the hoof body lotion shelf-life of the present invention, up to 42-54 month, extends 18-30 month than commercially available (24 months), also illustrating that inherent quality of the present invention is better, drug effect is safe and stable simultaneously.2) hoof body lotion of the present invention can effectively prevent the generation that the various body of milch cow is sick, especially ulcer, wart dermatitis and other hoof disease at the bottom of foot rot, hoof is had effect of preventing epidemic preferably, and compared with commercially available hoof body lotion, its hoof disease incidence rate reduces by 39.14%.Also illustrate that hoof body lotion of the present invention has good permeability, caking property, high efficiency simultaneously.3) various Milk cow hoof disease can quickly, effectively be treated by hoof body lotion of the present invention, and claw ill has the effect of antibacterial infection, removing toxic substances convergence, removing the necrotic tissue and promoting granulation and promotion wound healing, and permeability is strong, drug effect is high compared with commercial hoof body lotion, caking property is strong.When medication 7 days, its cure rate reached 65.7-80%, total effective rate reaches 88.6-94%；Respectively than the high 49.7-66.7% of matched group (13.3-16%) and (55-80%) and 8.6-39%；When medication 14 days, its cure rate reached 90-96%, total effective rate reaches 96.7-100%；Higher 14-36% and (70-86%) 10.7-30% than matched group (60-76%) respectively；Reach same therapeutic efficiency, treatment time at least shortens 7 days, particularly to wart dermatitis, foot rot and other type of hoof disease therapeutic effect are best, illustrate simultaneously, therapeutic process is not when using antibiotics auxiliary treatment, hoof body lotion treatment time of the present invention is short, efficiency is high, not only reduce milk cattle cultivating cost, add fanning economics, and improve the foodsafety of milch cow deep processed product, also protect breeding environment simultaneously, stop because using antibiotic, the potential safety hazard that chemistry and noxious substance produce, a new green shortcut is opened for application hoof body lotion treatment Milk cow hoof disease.4) trend that milch cow Body Condition Score is significantly improved by hoof body lotion of the present invention, it is possible to improve milch cow to the digestion of nutrient substance, absorption and metabolism, may advantageously facilitate and improve the synthesis of the framework material such as vivo protein, fat, hence it is evident that the body condition of milch cow can be improved.5) result of the test shows: test group is compared with matched group, and head day increases milk yield 7.38 kilograms, improves 19.23%, and t checks significant difference (P < 0.05), and hoof body lotion is remarkably improved the milk yield of lactating cow.6) Immunoglobulin IgA in cow serum, IgM, IgG content be the important indicator judging milch cow humoral immunity level.As seen from the experiment, cow birth stress cause that serum immune globulin IgA, IgM, IgG content sharply decline, and humoral immune function is suppressed.Test group hoof body lotion can increase before cow birth after 1 week, the childbirth same day and childbirth the content of 1 week serum IgA, IgM, IgG, enhancing humoral immune function.7) compared with matched group, test group Diseases of Cow can be greatly shortened nearly 15 days of the time of milch cow postpartum heat, improve the conception rate 20.5% of the first feelings phase milch cow in puerperal, increase the ratio of oestrusing 18.5% of Postpartum Cows colony, be effectively improved the reproductive performance of milch cow.8) test group is compared with matched group, in prevention postpartum disease of cow, the sickness rate of various disease can be significantly reduced, on average reduce 10-28.7%, t checks significant difference (P < 0.05), hoof body lotion can significantly reduce the incidence rate of the diseases such as milch cow mastitis in puerperal, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and placenta retention, improves the health status of milch cow.

Concrete know-why is as follows:

null1. the extracts of Chinese herbal medicine that prepared by the present invention is effectively to kill and suppress Milk cow hoof disease infectivity pathogen，And there is promoting blood circulation and stopping pain、Detumescence and promoting granulation、Dampness is stopped blooding、Parasite killing detoxifies、Removing dampness、Sore、Spasmolytic、Dispel heat，Can effectively prevent and treat multiple hoof disease、The Chinese medicine improving milch cow autoimmunity and premunition is that raw material is through ultrasonic cleaning、Ultrasonic assistant microwave extraction、Low temperature enzymolysis and water extracting alcohol carry and prepare，Extracting solution active constituent content is high，Medical material utilization rate is high、Extraction time is short、Extraction ratio is high、Extraction cost is low，Drug effect highly effective and safe，Can effectively prevent and prevent various Milk cow hoof disease，What be different from prior art is，While reaching better prevention and therapeutic effect，Science of the present invention is composite various conventional、Effective and that the property of medicine is soft natural Chinese medicinal herb，Without Calomelas、Realgar、The severe toxicity Chinese medicine such as Plumbum preparatium，Also without using any chemical drugs and antibiotic，Prevent it from entering blood circulation by wound，Further increase milk、The foodsafety of the deep processed products such as beef，Improve the safety of the medicine use of poultry feeders and the safety of breeding environment simultaneously，Protect environment.Above-mentioned drug effect is the synergistic result of each herbal component, not the superposition of single component drug effect.

null2. the Aloe extract of the preparation of the present invention is with Aloe for primary raw material，The composite Citrus pericarp of science、Pericarpium Vitis viniferae、Fructus Crataegi marc is through ultrasonic cleaning、High-voltage pulse extracts、Hot water extraction and prepared，Remain Aloe gel therein to greatest extent、Pectin、Aloe-emodin、Aloin A、Wound hormone and polysaccharide peptide manna、The multiple bioactive ingredients such as Aloe polysaccharide，Its viscosity is considerably beyond the viscosity of single Aloe gel and pectin，Substantially increase the caking property of hoof body lotion，The more important thing is that the multiple bioactive ingredients in Aloe can promote that wound healing and restoration ecosystem do not stay scar，Powerful reparation horny layer，Viral infection resisting、Pesticidal and sterilizing、Sun-proof anticancer、Defying age removing toxic substances，There is multiple efficacies，May additionally facilitate and carry other effective ingredient and penetrate into hoof deep layer，The osmotic efficiency of raising itself and Chinese herbal medicine，Shorten time of penetration，Work in coordination with other compositions，Such as: modified dietary fiber、Plant extract、Ovum Gallus domesticus album etc.，Its drug effect and serviceability are higher，It is especially suitable for the drug effect of milk Ungula Bovis seu Bubali body lotion、Safety and administering mode and method requirement.

3. the plant extract that prepared by the present invention is can change milch cow hoof horny layer structure, to promote that the effective ingredient rapid osmotic such as Chinese herbal medicine to deep skin and absorb, with effect a radical cure focus natural plants for raw material, the extract at low temperature techniques such as science is composite and cleaned, moistening, freezing and pulverizing, high-pressure pulse electric extraction, ultrasonic assistant microwave extraction, biological enzymolysis and prepare, such as: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae etc.；Its active constituent content is high; employing natural plant extracts is penetration enhancer; instead of the chemistry penetration enhancer such as the azone of prior art, dimethyl sulfone, tween, propylene glycol, ethanol completely and short saturating effect is more significantly; not only increase the permeability of hoof body lotion; and improve the pharmacological property of hoof body lotion; add the safety in utilization of hoof body lotion simultaneously, protect environment.Working in coordination with extracts of Chinese herbal medicine, its drug effect, safety and usability are higher.

4. the Ovum Gallus domesticus album that the present invention uses, after high-speed stirred, its volume can increase at least 6 times, it is a kind of excellent flocculant, flocculation material can be formed with part (such as: the Chinese medicine albumen) high molecular weight protein " combination " in component, be conducive to improving the storage stability of hoof body lotion, Ovum Gallus domesticus album has strong convergence effect simultaneously, can promoting wound healing, antiinflammatory, the pain that disappears pain relieving, prevent the effects such as suppuration, and Ovum Gallus domesticus album is easily in keratodermatitis film forming, improves adhesiveness and the efficacy of hoof body lotion.With other component synergistic action effects such as at, keratinase, modified dietary fibers more preferably.

5. the impact with strong retentiveness, dilatancy, thickening property, adsorptivity and acid and alkali, alkali, salt that dietary fiber is processed through physics, chemical or biological method and obtains by the modified dietary fiber that prepared by the present invention, significantly improves caking property and the physical and chemical stability of milk Ungula Bovis seu Bubali body lotion；High adsorption capacity, after modified, cellulosic specific surface area increases, network is enriched, absorption affinity strengthens, and further enhancing the caking property of hoof body lotion, and powerful embedding effect can prevent breeding environment (oxygen, temperature, illumination, the water activity etc.) factor impact on product drug effect, stabilize the property of medicine of hoof body lotion, extend shelf-life of hoof body lotion and prevent and useful life in therapeutic process, use the property of medicine.Working in coordination with Ovum Gallus domesticus album, trehalose, effect is more preferably.

6. science of the present invention is composite a small amount of keratinase and at, can decompose milch cow hoof horny layer appropriateness, increases effective ingredient rapid osmotic to internal layer, shortens treatment time and increases therapeutic effect；The horny layer that appropriateness is decomposed simultaneously is more prone to and Chinese herbal medicine effective ingredients, Aloe gel, pectin, Ovum Gallus domesticus album, modified dietary fiber bonding, fastness when further enhancing the caking property of hoof body lotion and use, difficult drop-off.

7. science of the present invention is composite trehalose, the Natural antioxidant, can effectively prevent the biological activitys such as keratinase, at, extracts of Chinese herbal medicine, Aloe extract, plant extract and effective ingredient oxidation, high temperature, freezing and lose activity and effect, enhance the stability of hoof body lotion, drug effect, permeability and caking property, finally improve safety and the usability of hoof body lotion, with the synergistic action effect such as modified dietary fiber, Ovum Gallus domesticus album more preferably.

null8. preparation method of the present invention overcomes traditional and existing hoof body lotion high temperature sterilization、Process time is long、Efficiency is low、Drug effect is low、The defects such as energy consumption is high，Omnidistance employing low temperature processing techniques，Effectively prevent the loss of material effective component，By ultrasonic dissolution、Dispersion、High pressure homogenize、The technology such as high-pressure pulse electric organically combine，Further enhancing the synergistic effect of material effective component and the caking property of hoof body lotion、Permeability and absorbability，After cold-heat treatment，Hoof body lotion transparency、Stability is higher，The shelf-life extending hoof body lotion is 42-54 month，18-30 month is extended than commercially available (24 months)，Shorten the process time，Improve work efficiency，Reduce production cost，Improve product quality，Improve fanning economics，Add the safety of milch cow deep-processed food，Protect environment.

It should be noted that the medicinal effects of hoof body lotion of the present invention is the result that each component is mutually worked in coordination with, interacted, the not superposition of simple raw material function, the science of each raw material components is composite and extracts the effect produced considerably beyond the superposition of each single component function and effect, has advanced preferably and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Prepared by embodiment 1 raw material

1. the preparation of extracts of Chinese herbal medicine:

The preparation method of described extracts of Chinese herbal medicine, comprises the steps: to count by weight, weighs Olibanum 23 parts, Sanguis Draxonis 19 parts, the Radix Astragali 15 parts, Myrrha 14 parts, Os Draconis 14 parts, Radix Achyranthis Bidentatae 10 parts, Cortex Acanthopancis 8 parts, Pulvis Fumi Carbonisatus 5 parts, Herba Portulacae 5 parts, Radix Notoginseng 3 parts, Cortex Dictamni 2 parts, Cortex Meliae 2 parts；Put in the ultrasonic washing unit filling 0.2% sodium bicarbonate solution and clean 12min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, puts Homogeneous phase mixing in container and adds the water of 5 times of weight, obtaining mixed material, control temperature 80 DEG C and keep 3h, then it is cooled to 45 DEG C, is 4.0 with breast acid for adjusting pH value, carries out microwave extraction 12min when power 200W, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 1.0% carries out enzymolysis, is 6.2 with breast acid for adjusting pH value, enzymolysis 3h, finally adding the mixture of 2 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:2, controls temperature and keeps 3.5h to 70 DEG C, filter, obtain the first filtrate；Add the water of 2 times of weight of filtering residue, control temperature 90 DEG C and keep 2h, be then cooled to 30 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 3:2, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

Described compound enzyme is glucanase, pentosanase, xylanase, acid protease, tannase 6:3:3:2:2 Homogeneous phase mixing in mass ratio.

2. the preparation of Aloe extract:

The preparation method of described Aloe extract, comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 8min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 23:4:3:2 Homogeneous phase mixing, then in-23 DEG C of freezing 20min；Pulverizing immediately, particle diameter is 0.5mm；Adding the deionized water of ground product quality 4 times, mix homogeneously, is 2.5 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 40kV/cm under room temperature, burst length 500 μ s, carries out high voltage pulse electric field processing when pulse frequency 300Hz, then normal pressure boils 40min, Bag filter, the filtering residue filtrate normal pressure of its quality 2 times boils 50min, Bag filter, merging filtrate, it is 8% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

3. the preparation of plant extract:

The preparation method of described plant extract, comprise the steps: Radix Angelicae Sinensis, Folium clausenae lansii, Flos Caryophylli, Radix Salviae Miltiorrhizae, Herba Menthae is respectively washed, drain, 11:8:8:5:2 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 5h that pH value is 5.0 of mixed material quality 0.4 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, ground product particle diameter 0.8mm, it is subsequently added into the water of ground product quality 5 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, high voltage pulse electric field processing 25min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 18min when power 200W in room temperature, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 1.0%, in 50 DEG C of enzymolysis 40min；Enzymolysis solution filters, filtrate reduced in volume to solid content is 9% namely obtain plant extract；

Described mixed enzyme is cellulase, hemicellulase, pectase, tannase, protease 4:4:3:2:0.4 Homogeneous phase mixing in mass ratio.

4. the preparation of modified dietary fiber:

The preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 7:4:3:2 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 200W, 35KHz condition supersound extraction 12min, then at electric field intensity 30kV/cm, burst length 400 μ s, carries out high voltage pulse electric field processing 12min when pulse frequency 300Hz；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.5%, in 50 DEG C of enzymolysis 35min；Enzymolysis solution filters, and filtrate reduced in volume to solid content is 6% namely obtain modified dietary fiber；

Described enzyme is cellulase, glucanase, xylanase, pectase 6:3:2:1.5 Homogeneous phase mixing in mass ratio.

Extracts of Chinese herbal medicine that example 2 below-6 uses, Aloe extract, plant extract, modified dietary fiber are embodiment 1 and prepare, and other is commercial commodity, and wherein trehalose, the Natural antioxidant, keratinase, at are powdery.

Embodiment 2

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts of Ovum Gallus domesticus album, the Natural antioxidant 2.5 parts, keratinase 2.5 parts, at 2.5 parts；

The quality group of the described Natural antioxidant becomes: grape pip procyanidin: tea polyphenols: Herba Rosmarini Officinalis extract=5:3:2；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, make outlet mixture particle diameter reach 150 μm, then pass through high pressure homogenizer in temperature 65 DEG C, circulate 3 times when pressure 110MPa so that homogenizer outlet material particle diameter is 130nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 16min in rotating speed 660r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 70 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 140r/min,Fully dissolve 25min,Stop stirring，Heat up，Normal pressure boils 8min，Naturally room temperature it is down to，Continue to be cooled to-1 DEG C，Insulation 2h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 3 times，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 110r/min,Homogeneous phase mixing，In power 300W,Ultrasonic Treatment 20min is carried out when frequency 40KHz,Simultaneously at electric field intensity 40kV/cm，Burst length 700 μ s,High voltage pulse electric field processing is carried out when pulse frequency 500Hz，Last in 0.3 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

It it is 54 months through the hoof body lotion shelf-life prepared by said method.

Embodiment 3

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 65 parts, Aloe extract 45 parts, plant extract 25 parts, modified dietary fiber 8 parts, trehalose 7 parts, 5 parts of Ovum Gallus domesticus album, the Natural antioxidant 2 parts, keratinase 2 parts, at 2 parts；

The quality group of the described Natural antioxidant becomes: grape pip procyanidin: tea polyphenols: Herba Rosmarini Officinalis extract=4:2:1；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, make outlet mixture particle diameter reach 100 μm, then pass through high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 100MPa so that homogenizer outlet material particle diameter is 100nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 12min in rotating speed 600r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 65 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 130r/min,Fully dissolve 20min,Stop stirring，Heat up，Normal pressure boils 5min，Naturally room temperature it is down to，Continue to be cooled to-1.5 DEG C，Insulation 1h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 2 times，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 100r/min,Homogeneous phase mixing，In power 200W,Ultrasonic Treatment 10min is carried out when frequency 35KHz,Simultaneously at electric field intensity 35kV/cm，Burst length 600 μ s,High voltage pulse electric field processing is carried out when pulse frequency 400Hz，Last in 0.2 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

It it is 52 months through the hoof body lotion shelf-life prepared by said method.

Embodiment 4

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 75 parts, Aloe extract 55 parts, plant extract 35 parts, modified dietary fiber 10 parts, trehalose 9 parts, 7 parts of Ovum Gallus domesticus album, the Natural antioxidant 3 parts, keratinase 3 parts, at 3 parts；

The quality group of the described Natural antioxidant becomes: grape pip procyanidin: tea polyphenols: Herba Rosmarini Officinalis extract=6:4:3；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, make outlet mixture particle diameter reach 200 μm, then pass through high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 120MPa so that homogenizer outlet material particle diameter is 150nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 20min in rotating speed 720r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 75 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 150r/min,Fully dissolve 30min,Stop stirring，Heat up，Normal pressure boils 10min，Naturally room temperature it is down to，Continue to be cooled to-0.5 DEG C，Insulation 3h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 4 times，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 120r/min,Homogeneous phase mixing，In power 400W,Ultrasonic Treatment 30min is carried out when frequency 45KHz,Simultaneously at electric field intensity 45kV/cm，Burst length 800 μ s,High voltage pulse electric field processing is carried out when pulse frequency 600Hz，Last in 0.4 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

It it is 50 months through the hoof body lotion shelf-life prepared by said method.

Embodiment 5

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 60 parts, Aloe extract 40 parts, plant extract 20 parts, modified dietary fiber 6 parts, trehalose 5 parts, 3 parts of Ovum Gallus domesticus album, tea polyphenols 1 part, keratinase 1 part, at 1 part；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, make outlet mixture particle diameter reach 100 μm, then pass through high pressure homogenizer in temperature 75 DEG C, circulate 4 times when pressure 100MPa so that homogenizer outlet material particle diameter is 100nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 12min in rotating speed 720r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 75 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 130r/min,Fully dissolve 30min,Stop stirring，Heat up，Normal pressure boils 5min，Naturally room temperature it is down to，Continue to be cooled to-0.5 DEG C，Insulation 1h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 4 times，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 100r/min,Homogeneous phase mixing，In power 400W,Ultrasonic Treatment 30min is carried out when frequency 35KHz,Simultaneously at electric field intensity 35kV/cm，Burst length 800 μ s,High voltage pulse electric field processing is carried out when pulse frequency 400Hz，Last in 0.2 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

It it is 42 months through the hoof body lotion shelf-life prepared by said method.

Embodiment 6

A kind of antibiotic-free milk Ungula Bovis seu Bubali body lotion, is mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 80 parts, Aloe extract 60 parts, plant extract 40 parts, modified dietary fiber 12 parts, trehalose 11 parts, 9 parts of Ovum Gallus domesticus album, grape pip procyanidin 4 parts, keratinase 4 parts, at 4 parts；

Preparation method, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, make outlet mixture particle diameter reach 200 μm, then pass through high pressure homogenizer in temperature 55 DEG C, circulate 2 times when pressure 120MPa so that homogenizer outlet material particle diameter is 150nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 20min in rotating speed 600r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 65 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 150r/min,Fully dissolve 20min,Stop stirring，Heat up，Normal pressure boils 10min，Naturally room temperature it is down to，Continue to be cooled to-1.5 DEG C，Insulation 3h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 2 times，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 120r/min,Homogeneous phase mixing，In power 200W,Ultrasonic Treatment 10min is carried out when frequency 45KHz,Simultaneously at electric field intensity 45kV/cm，Burst length 600 μ s,High voltage pulse electric field processing is carried out when pulse frequency 600Hz，Last in 0.2 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

It it is 46 months through the hoof body lotion shelf-life prepared by said method.

Embodiment 7 hoof body lotion storage stability test of the present invention

Hoof body lotion is as a kind of liquid preparation mainly prepared by extracts of Chinese herbal medicine, stability criterion with reference to liquid preparation of Chinese medicine, clarity is defined as the quality stability index of hoof body lotion, it not only directly reflects the presentation quality of product, also reflect product inherent quality and stability, be the most important quality control index of hoof body lotion.

Clarity characterizing method: clear 90-100 divides；Micro-turbid 80-89 divides；Muddy 70-79 divides；More muddy 60-69 divides；Serious muddy 50-59 divides；Precipitate less than 49 points (deposit seed is more big, quantity is more many, and mark is more low).

Hoof body lotion prepared by the embodiment of the present invention 2 and commercially available same type, same specification, with the hoof body lotion of date of manufacture in room temperature, ventilation, lucifuge condition storage, observe hoof body lotion clarity, once, result is in Table 1 for every 6 months observed and recorded results:

Table 1: shelf time hoof body lotion clarity testing result

Time (moon)	6	12	18	24	30	36	42	48	54	Always divide equally
											Commercially available	95	90	85	78	73	65	55	45	30	68.44
The present invention	100	98	95	93	93	92	90	82	81	91.56

Result above shows: it is better that hoof body lotion of the present invention compares presentation quality with commercially available hoof body lotion, clarity is high, prolongation over time, hoof body lotion liquid phase is relatively unstable, according to use and the drug effect standard of hoof body lotion, the hoof body lotion shelf-life of the present invention, up to 42-54 month, extends 18-30 month than commercially available (24 months), also illustrating that inherent quality of the present invention is better, drug effect is safe and stable simultaneously.

It should be understood that hoof body lotion prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

Embodiment 8 hoof body lotion of the present invention prevention Milk cow hoof disease epidemic prevention test

1 materials and methods

1.1, test material

1.1.1, the embodiment of the present invention 2 preparation hoof body lotion and commercially available hoof body lotion

1.1.2, milch cow is tested: a certain cattle farm carries out in Miyun Region of Beijing in test, and kind is the china holstein cows 2 above Diseases of Cow of tire, totally 46, all without hoof disease, wherein 16,2 tire, 3 12, tires, 4 10, tires, 58, tires.Test cattle, by the same or like pair principle of parity, early stage milk yield, is divided into experimental group and matched group, often group 23.

1.2, test daily ration composition: test group and matched group adopt same feeding and management method.Daily ration consists of concentrate (Semen Maydis 52.7%, wheat bran 8%, bean cake 7%, cottonseed meal 9%, Semen arachidis hypogaeae dregs 9%, corn DDGS 8%, premix material 7115A6%), and coarse fodder is corn silage, Leymus chinensis (Trin.) Tzvel., the bean stalks of rice, wheat, etc., Herba Medicaginis etc..

1.3, test method: test group and matched group be hoof bath epidemic prevention hoof disease respectively, test group uses the hoof body lotion of the embodiment of the present invention 2 preparation, matched group uses commercially available hoof body lotion, using method: prevent requirement according to hoof disease, thoroughly clean Ungula Bovis seu Bubali portion, guarantee that hoof and interdigit do not have feces to remain, so as to it is clean, hoof body lotion is configured to the medicinal liquid that mass percent concentration is 5%, it is subsequently adding in special milk Ungula Bovis seu Bubali bath, liquid level is 15cm at least, milch cow hoof soaks 25min in special hoof bath and namely completes a hoof bath, hoof body lotion is changed when same hoof bath hoof bathes 200 times.Every day is sooner or later each is once a preventative epidemic prevention cycle for three days on end, quarterly an epidemic prevention cycle；6) a situation arises for observation during test, record test group and the various hoof disease of matched group milch cow, will add up hoof disease incidence rate the end of the year, and result is table 2 such as；All for the concentrate 4-8kg of giving milk of autogamy in examination Cow-feeding field, it is adjusted according to raising stage and milk yield, corn silage quantitative feeding 20kg/ days, roughage (Leymus chinensis (Trin.) Tzvel., the bean stalks of rice, wheat, etc., Herba Medicaginis) free choice feeding.

1.4, test period: June 11 in 2015 experimental period from June 12nd, 2014 end of day, last 1 year.

Table 2: Milk cow hoof disease epidemic prevention result

Result above shows: hoof body lotion of the present invention can effectively prevent the generation that the various body of milch cow is sick, especially ulcer, wart dermatitis and other hoof disease at the bottom of foot rot, hoof is had effect of preventing epidemic preferably, and compared with commercially available hoof body lotion, its hoof disease incidence rate reduces by 39.14%.Also illustrate that hoof body lotion of the present invention has good permeability, caking property, high efficiency simultaneously.

The embodiment 9 hoof body lotion of the present invention effect test when treating Milk cow hoof disease

For verifying the clinical efficacy of hoof body lotion of the present invention, three dairy cow farms in Hebei province and Inner Mongolia Autonomous Region in October ,-2015 in June, 2014, select 320 example Milk cow hoof diseases altogether, wherein wart dermatitis 100 example, hypertrophy 60 example between toe, ulcer 70 example at the bottom of hoof, foot rot 50 example, other type hoof disease 40 example, it is randomly divided into two groups: test group and matched group according to hoof disease type, hoof body lotion prepared by the embodiment of the present invention 2 and commercial hoof body lotion is not used to treat through plant veterinary and foot paring work point, test group and matched group are except medication (hoof body lotion) difference, other is raised and way to manage, daily ration, Therapeutic Method, feed environment, efficacy evaluations etc. are all identical.

Case selection standard: having symptom of substantially walking lamely, happiness is lain prone sleeping, and dysstasia or stand up time are shorter；The suffering limb unreal or each limb hoof of heavy burden alternately bears a heavy burden；Palpation suffering limb hoof temperature rise, pain sensitivity during percussion.Sick cattle performance spirit is depressed, and inappetence, milk yield declines, and body temperature raises sometimes, up to more than 40 DEG C.Such as aggravation, indivedual ill milch cows show some General Symptomies, and severe one affected part is red and swollen, does not rise sleepingly.

Using method: treat requirement according to hoof disease, thoroughly clean Ungula Bovis seu Bubali portion, guarantee that hoof and interdigit do not have feces to remain, so as to it is clean, hoof body lotion being configured to the medicinal liquid that mass percent concentration is 7%, is subsequently adding in special milk Ungula Bovis seu Bubali bath, liquid level is 15cm at least, milch cow hoof soaks 25min in special hoof bath and namely completes a hoof bath, changes hoof body lotion when same hoof bath hoof bathes 200 times.Every day, sooner or later respectively once the bath of continuous hoof 4 days was a treatment cycle, and test group and matched group respectively carry out a treatment cycle.

Curative effect determinate standard: observe sick cattle symptom, determine curative effect according to criterion for the 7th day, 14 days after hoof is bathed.Suffering from cattle limping to disappear, pain disappears, and swelling is disappeared, and wound healing is for curing；Pain substantially alleviates, swelling range shorter, walk lamely alleviate or inconspicuous be effective；Pain does not disappear, and swelling, without disappearing, is walked lamely without disappearing, had dyskinetic for invalid.Total effectively head number is equal to recovery from illness and effective head number summation.

The result for the treatment of is carried out statistics and analysis, specifically as shown in Table 3, 4:

Table 3: hoof 7 days therapeutic outcomes of bath

Table 4: hoof 14 days therapeutic outcomes of bath

Result above shows: various Milk cow hoof disease can quickly, effectively be treated by hoof body lotion of the present invention, claw ill has the effect of antibacterial infection, removing toxic substances convergence, removing the necrotic tissue and promoting granulation and promotion wound healing, and permeability is strong, drug effect is high compared with commercial hoof body lotion, caking property is strong.When hoof is bathed 7 days, its cure rate has reached 65.7-80%, total effective rate reaches 88.6-94%；Respectively than the high 49.7-66.7% of matched group (13.3-16%) and (55-80%) and 8.6-39%；When hoof is bathed 14 days, its cure rate has reached 90-96%, total effective rate reaches 96.7-100%；Higher 14-36% and (70-86%) 10.7-30% than matched group (60-76%) respectively；Reach same therapeutic efficiency, treatment time at least shortens 7 days, particularly to wart dermatitis, foot rot and other type of hoof disease therapeutic effect are best, illustrate simultaneously, therapeutic process is not when using antibiotics auxiliary treatment, hoof body lotion treatment time of the present invention is short, efficiency is high, not only reduce milk cattle cultivating cost, add fanning economics, and improve the foodsafety of milch cow deep processed product, also protect breeding environment simultaneously, stop because using antibiotic, the potential safety hazard that chemistry and noxious substance produce, a new green shortcut is opened for application hoof body lotion treatment Milk cow hoof disease.

The impact on milk cow production performance, reproductive performance and anti-disease of the embodiment 10 hoof of the present invention body lotion

1 materials and methods

1.1, test material

1.1.1, product of the present invention

1.1.2, test milch cow: embodiment 9 experimental group and matched group are through treating the milch cow of hoof disease.

1.3, test method: according to milch cow breeding record, test group is except taking same method to feed with matched group, all for the concentrate 4-8kg of giving milk of autogamy in examination Cow-feeding field, it is adjusted according to raising stage and milk yield, corn silage quantitative feeding 20kg/ days, roughage (Leymus chinensis (Trin.) Tzvel., the bean stalks of rice, wheat, etc., Herba Medicaginis) free choice feeding.

1.4, production, metabolism, reproductive performance record: in during 120 days puerperal, measure and record the milk yield of every cow head；Observe, evaluate milch cow body condition；Detection reproductive performance；During 120 days puerperal, observed and recorded is participated in the experiment the rutting situation of milch cow, and whether gestation is as the criterion with examination per rectum in latter 60 days of breeding.

1.5, disease surveillance and record: to Dairy Cows diseases such as () displacement of mastitis, endometritis, ketoacidosis, cud, intractable apositia, lochiopyra and placenta retention detection, the same day was monitored placenta retention incidence in childbirth, and definition afterbirth not discharge person in 12-24 hour is placenta retention.Adopt Ketone benzene detection Ketosis, adopt CMT-Test reagent detection mastitis.

1.6, initial data is processed by statistical analysis EXCEL software, then carries out variance analysis by SAS [11] method.

2. result of the test

The impact on milch cow body condition of the 2.1 hoof body lotions:

Table 5 milch cow 5 part Body Condition Score standard

Score value	Vertebra portion	Flank	Buttocks both sides	Root of the tail both sides	Hipbone, ischium joint
						1	Very prominent	Root root is visible	Excessive settlement	Crypts is very deep	Very prominent
2	Substantially prominent	Most visible	Substantially it sink	Crypts is obvious	Substantially prominent
						3	Somewhat prominent	Minority is visible	Somewhat sagging	Crypts is slightly aobvious	Somewhat prominent
4	Straight	Lose completely	Straight	Crypts does not show	Do not show prominent
						5	Plentiful	Plentiful	Plentiful	Plentiful	Plentiful

Table 6 milch cow body condition visual examination appraisal result

Result above shows: the trend that milch cow Body Condition Score is significantly improved by test group hoof body lotion, milch cow can be improved to the digestion of nutrient substance, absorption and metabolism, may advantageously facilitate and improve the synthesis of the framework material such as vivo protein, fat, hence it is evident that the body condition of milch cow can be improved.

The impact on milk production of cow of the 2.2 hoof body lotions

6120 days milk production of cow of table

	Head number (head)	Give milk natural law (my god)	Total milk yield (kg)	Average milk production (kg/ days. head)
					Matched group	160	120	736704	38.37±5.16
Test group	160	120	874560	45.75±5.92
					Process difference^b				7.38

Result above shows: test group is compared with matched group, and head day increases milk yield 7.38 kilograms, improves 19.23%, and t checks significant difference (P < 0.05), and hoof body lotion is remarkably improved the milk yield of lactating cow.

The impact on immunity of cow of the 2.3 hoof body lotions

Table 7 Diseases of Cow Immunoglobulins in Serum testing result

Immunoglobulin IgA in cow serum, IgM, IgG content be the important indicator judging milch cow humoral immunity level.As seen from the experiment, cow birth stress cause that serum immune globulin IgA, IgM, IgG content sharply decline, and humoral immune function is suppressed.Test group hoof body lotion can increase before cow birth after 1 week, the childbirth same day and childbirth the content of 1 week serum IgA, IgM, IgG, enhancing humoral immune function.

The impact on Diseases of Cow reproductive performance of the 2.4 hoof body lotions

Table 8 Diseases of Cow reproductive performance testing result

Result of the test shows, compared with matched group, test group Diseases of Cow can be greatly shortened nearly 15 days of the time of milch cow postpartum heat, improves the conception rate 20.5% of the first feelings phase milch cow in puerperal, increase the ratio of oestrusing 18.5% of Postpartum Cows colony, be effectively improved the reproductive performance of milch cow.

The impact that Diseases of Cow puerperal disease is occurred by 2.5 hoof body lotions

Table 9 Diseases of Cow puerperal disease testing result

Result above shows: test group is compared with matched group, in prevention postpartum disease of cow, the sickness rate of various disease can be significantly reduced, on average reduce 10-28.7%, t checks significant difference (P < 0.05), hoof body lotion can significantly reduce the incidence rate of the diseases such as milch cow mastitis in puerperal, endometritis, ketoacidosis, cud displacement, intractable apositia, lochiopyra and placenta retention, improves the health status of milch cow.

To sum up, use hoof body lotion of the present invention to be remarkably improved milch cow physique, immunity production performance, reproductive performance and the various disease of prevention, effectively prevent the harm of cultivation directly or indirectly brought because of Milk cow hoof disease,

Claims

1. an antibiotic-free milk Ungula Bovis seu Bubali body lotion, mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 60-80 part, Aloe extract 40-60 part, plant extract 20-40 part, modified dietary fiber 6-12 part, trehalose 5-11 part, Ovum Gallus domesticus album 3-9 part, Natural antioxidant 1-4 part, keratinase 1-4 part, at 1-4 part；

Described plant extract is can change milch cow hoof horny layer structure, to promote that hoof body lotion component rapid osmotic is prepared for raw material to deep skin the natural plants that absorbs.

2. antibiotic-free milk Ungula Bovis seu Bubali body lotion as claimed in claim 1, it is characterized in that, mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 65-75 part, Aloe extract 45-55 part, plant extract 25-35 part, modified dietary fiber 8-10 part, trehalose 7-9 part, Ovum Gallus domesticus album 5-7 part, Natural antioxidant 2-3 part, keratinase 2-3 part, at 2-3 part.

3. antibiotic-free milk Ungula Bovis seu Bubali body lotion as claimed in claim 1, it is characterized in that, mainly prepared by the raw material of following parts by weight: extracts of Chinese herbal medicine 70 parts, Aloe extract 50 parts, plant extract 30 parts, modified dietary fiber 9 parts, trehalose 8 parts, 6 parts of Ovum Gallus domesticus album, the Natural antioxidant 2.5 parts, keratinase 2.5 parts, at 2.5 parts.

4. the antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-3, it is characterised in that the preparation method of described extracts of Chinese herbal medicine, comprise the steps: to count by weight, weigh Olibanum 20-25 part, Sanguis Draxonis 18-20 part, Radix Astragali 13-16 part, Myrrha 12-15 part, Os Draconis 12-15 part, Radix Achyranthis Bidentatae 8-12 part, Cortex Acanthopancis 7-9 part, Pulvis Fumi Carbonisatus 4-6 part, Herba Portulacae 4-6 part, Radix Notoginseng 2-4 part, Cortex Dictamni 1-3 part, Cortex Meliae 1-3 part；Put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution and clean 10-15min in 200W, 30KHz, drain, above-mentioned Chinese medicine powder is broken to particle diameter is below 2mm, put Homogeneous phase mixing in container and add the water of 3-6 times of weight, obtain mixed material, control temperature 70-90 DEG C and keep 2-4h, then 40-50 DEG C it is cooled to, it is 3.5-4.5 with breast acid for adjusting pH value, microwave extraction 10-15min is carried out when power 150-300W, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；The compound enzyme being subsequently adding mixed material gross weight 0.5-1.5% carries out enzymolysis, it is 5.5-6.8 with breast acid for adjusting pH value, enzymolysis 2-4h, finally add the mixture of 0.5-3 times of w ethanol of mixed material and propanol, the mass ratio of ethanol and propanol mixing is 1:1-3, control temperature and keep 3-4h to 60-78 DEG C, filter, obtain the first filtrate；Add the water of 1-3 times of weight of filtering residue, control temperature 85-95 DEG C and keep 1-3h, be then cooled to 25-35 DEG C, filter, obtain the second filtrate；First filtrate and the second filtrate are merged according to mass ratio 2-4:1-3, Homogeneous phase mixing, obtain extracts of Chinese herbal medicine；

5. the antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described Aloe extract, comprise the steps: fresh Aloe, Citrus pericarp, Pericarpium Vitis viniferae, Fructus Crataegi marc to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 20-25:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；Adding the deionized water of ground product quality 3-5 times, mix homogeneously, is 2-3 with Fructus Citri Limoniae acid for adjusting pH value, at electric field intensity 35-45kV/cm under room temperature, burst length 400-600 μ s, carries out high voltage pulse electric field processing when pulse frequency 200-400Hz, then normal pressure boils 30-50min, Bag filter, filtering residue boils 40-60min, Bag filter, merging filtrate with its filtrate normal pressure of quality 1-3 times, it is 6-10% that filtrate is evaporated to solid content at 75 DEG C, obtains Aloe extract.

null6. the antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-3，It is characterized in that，The preparation method of described plant extract，Comprise the steps: Radix Angelicae Sinensis、Folium clausenae lansii、Flos Caryophylli、Radix Salviae Miltiorrhizae、Herba Menthae is respectively washed、Drain，10-12:7-9:7-9:4-6:1-3 Homogeneous phase mixing in mass ratio，Add the lactic acid moistening 3-8h that pH value is 4.5-5.5 of mixed material quality 0.3-0.5 times,Pulverize immediately after-18--22 DEG C of freezing 1-2h，Freezing thickness of feed layer 3-5cm，Ground product particle diameter 0.5-1mm,It is subsequently added into the water of ground product quality 4-6 times，It is 3.5-5.5 with breast acid for adjusting pH value，At electric field intensity 25-35kV/cm under room temperature，Burst length 300-500 μ s,High voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz；Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 0.9-1.1%, in 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate reduced in volume to solid content is that namely 8-10% obtains plant extract；

7. the antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, apple fiber, Herba avenae fatuae fiber 6-8:3-5:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carries out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.4-0.6%, in 45-55 DEG C of enzymolysis 25-45min；Enzymolysis solution filters, and filtrate reduced in volume to solid content is that namely 5-8% obtains modified dietary fiber；

8. the antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-3, it is characterised in that the described Natural antioxidant contains any one or a few in tea polyphenols, grape pip procyanidin, Herba Rosmarini Officinalis extract, Radix Glycyrrhizae extract, Folium Pruni extract and Vc.

9. the preparation method of antibiotic-free milk Ungula Bovis seu Bubali body lotion as described in as arbitrary in claim 1-8, it is characterized in that, comprise the steps: according to formula, first by extracts of Chinese herbal medicine, Aloe extract and plant extract Homogeneous phase mixing, put in colloid mill, regulate the gap between stator and rotor, outlet mixture particle diameter is made to reach 100-200 μm, then pass through high pressure homogenizer in temperature 55-75 DEG C, circulate 2-4 time when pressure 100-120MPa, making homogenizer outlet material particle diameter is 100-150nm, obtains nanoparticle mixed liquor；nullOvum Gallus domesticus album is put in blender simultaneously and stir 12-20min in rotating speed 600-720r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Then intensification nanoparticle mixed liquor is to 65-75 DEG C，Insulation，It is sequentially added into egg white foams while stirring、Modified dietary fiber、Trehalose，Speed of agitator 130-150r/min,Fully dissolve 20-30min,Stop stirring，Heat up，Normal pressure boils 5-10min，Naturally room temperature it is down to，Continue to be cooled to-1.5-0.5 DEG C，Insulation 1-3h，Repeat above-mentioned normal pressure to boil and low-temperature insulation operation 2-4 time，The Natural antioxidant it is sequentially added into while stirring under room temperature、At and keratinase，Speed of agitator 100-120r/min,Homogeneous phase mixing，In power 200-400W,Ultrasonic Treatment 10-30min is carried out when frequency 35-45KHz,Simultaneously at electric field intensity 35-45kV/cm，Burst length 600-800 μ s,High voltage pulse electric field processing is carried out when pulse frequency 400-600Hz，Last in 0.2-0.4 μm of micro-pore-film filtration，Remove flocculate，Namely filtrate sterile filling obtains antibiotic-free milk Ungula Bovis seu Bubali body lotion.

10. the preparation method of antibiotic-free milk Ungula Bovis seu Bubali body lotion as claimed in claim 9, it is characterised in that the quality group of the described Natural antioxidant becomes: grape pip procyanidin: tea polyphenols: Herba Rosmarini Officinalis extract=4-6:2-4:1-3.