CN105738171A - Method for quantitatively and uniformly adhering algae on surface of silicone rubber test piece - Google Patents
Method for quantitatively and uniformly adhering algae on surface of silicone rubber test piece Download PDFInfo
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- CN105738171A CN105738171A CN201511000588.1A CN201511000588A CN105738171A CN 105738171 A CN105738171 A CN 105738171A CN 201511000588 A CN201511000588 A CN 201511000588A CN 105738171 A CN105738171 A CN 105738171A
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- silicone rubber
- algae
- test piece
- rubber test
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
Abstract
The invention discloses a method for quantitatively and uniformly adhering algae on the surface of a silicone rubber test piece. The method comprises the following steps: (1) putting a silicone rubber test piece into a container, and attaching the test piece on the bottom surface of the container, wherein the shape of the silicone rubber test piece is identical with the shape of the bottom surface of the container, and the gap between the silicone rubber test piece and the lateral wall of the container is less than 0.5 mm; (2) according to the predetermined density of algae on the surface of the silicone rubber test piece, and adding a certain volume of algae solution with a known density into the container, wherein the liquid level of the added algae solution is higher than the upper surface of the silicone rubber test piece; (3) making the upper surface of the silicone rubber test piece and the liquid surface of the algae solution be horizontal so as to obtain a silicone rubber test piece where algae adheres uniformly and quantitatively, after the algae solution is dried. The hydrophobicity of the surface of the obtained silicone rubber test piece can be directly measured by a contact angle method, and thus the influence of adhered algae on the surface hydrophobicity of silicone rubber can be researched quantitatively and systematically.
Description
Technical field
The present invention relates to high voltage and insulation technology, particularly relate to a kind of at silicone rubber strip quantitatively, the method for periphtic algae equably.
Background technology
Silastic material plays highly important effect because of its prominent hydrophobicity performance in high voltage external insulation field.In ultra-high-tension power transmission line, the composite insulator being fabricated to silastic material is very common, and its effect is to hang wire, it is ensured that reliably insulate between wire and the shaft tower of electronegative potential of high potential；In transformer station, post insulator sub-surface also extensively sprays room temperature vulcanized silicone rubber (RTV) material.
The surface of silastic material is hydrophobic surface, significantly improves insulating reliability.But in China Sichuan, Yunnan, Guangxi, isothermal Humid Area, Hunan, the RTV coating surface of composite insulator and insulation column have growth algae phenomenon.On-the-spot test finds, the growth of algae can destroy the hydrophobicity of material surface, reduces its insulating reliability.
The method of research insulant surface hydrophobicity mainly has two kinds: HC staging and contact angle method.In the research of domestic and international algae relevant issues, adopt HC stagings more, and the method is used for the overall hydrophobic situation of on-site measurement insulator, relies on the subjective judgment of gauger.And contact angle method can hydrophobic situation objective, quantitative analysis insulant surface exactly, but measurand is usually required that it is plane, and requires to be attached to the material on insulant surface as far as possible uniformly, be therefore used for laboratory research.
Contact angle method is the research algae method for optimizing to the affecting laws of insulant surface hydrophobicity, but by algae quantitatively, be uniformly adhered to the key that insulant strip is problem, and laboratory cultures algae multipurpose liquid culture medium, if simply algae solution being dropped in the silastic material surface of hydrophobic, algae solution can present the form of the water droplet of separation, uniform surface cannot be formed, do not meet the requirement of Contact-angle measurement.
Summary of the invention
The technical problem to be solved is: propose a kind of at silicone rubber strip quantitatively, the method for periphtic algae equably, utilize the silicone rubber test piece that the method obtains to be used directly for contact angle method and measure the hydrophobicity performance on its surface, and then quantitatively, systematically study the algae impact for silastic material surface hydrophobic performance.
The technical problem of the present invention is solved by following technical scheme:
A kind of at silicone rubber strip quantitatively, the method for periphtic algae equably, comprise the steps:
(1) silicone rubber test piece being put into a container the described container bottoms that reclines, the shape of described silicone rubber test piece is identical with the shape of described container bottoms, and the gap between described silicone rubber test piece and described container side wall is within 0.5mm；
(2) according to predetermined silicone rubber strip algae cell density, adding the algae solution of the known algae cell density of certain volume in described container, the liquid level of the algae solution of addition is higher than the upper surface of described silicone rubber test piece；
(3) upper surface of described silicone rubber test piece and the liquid level of described algae solution are all kept level, treat that described algae solution is dried, obtain being attached to quantitatively, equably the silicone rubber test piece of algae.
Preferably, the volume following formula of the algae added in described step (2) calculates:
Wherein: V represents the volume of the algae of addition；A represents silicone rubber strip algae cell density；C represents the algae cell density in algae solution；S represents the upper surface area of silicone rubber test piece or the inner surface area of described container bottoms.
Preferably, the thickness of described silicone rubber test piece is 1mm-5mm.
Preferably, predetermined in described step (2) silicone rubber strip algae cell density is not less than 2 × 107Individual/cm2。
Preferably, described container is cylinder or cube.
Preferably, the material of described container is colourless transparent glass.
Preferably, in described step (2), the algae cell density of algae solution is calculated after being counted by blood counting chamber and obtains.
The beneficial effect that the present invention is compared with the prior art includes: can at the surface basis weight of silicone rubber test piece and periphtic algae equably by the method for the present invention, utilize the silicone rubber test piece that the method obtains to be used directly for contact angle method and measure the hydrophobicity performance on its surface, by equably and different quantitative attachment, can systematically study the algae impact on silastic material surface hydrophobic performance, such that it is able to when testing the hydrophobicity performance of insulator surface at the scene, accurately determine the algae impact on it in the overall hydrophobicity performance test result of insulator.
Accompanying drawing explanation
Fig. 1 is that in one embodiment of the present invention, the front elevational schematic after in container is put in silicone rubber test piece.
Detailed description of the invention
Below in conjunction with detailed description of the invention and compare accompanying drawing the present invention is described in further details.
The present invention provide a kind of at silicone rubber strip quantitatively, the method for periphtic algae equably, in a specific embodiment, comprise the steps:
(1) silicone rubber test piece being put into a container the described container bottoms that reclines, the shape of described silicone rubber test piece is identical with the shape of described container bottoms, and the gap between described silicone rubber test piece and described container side wall is within 0.5mm；
(2) according to predetermined silicone rubber strip algae cell density, adding the algae solution of the known algae cell density of certain volume in described container, the liquid level of the algae solution of addition is higher than the upper surface of described silicone rubber test piece；
(3) upper surface of described silicone rubber test piece and the liquid level of described algae solution are all kept level, treat that described algae solution is dried, obtain being attached to quantitatively, equably the silicone rubber test piece of algae.
In some preferred embodiments, the volume following formula of the algae added in step (2) calculates:
Wherein: V represents the volume of the algae of addition, unit can be set to mL；A represents silicone rubber strip algae cell density, and unit can be set to individual/cm2；C represents the algae cell density in algae solution, and unit can be set to individual/mL；S represents the upper surface area of silicone rubber test piece or the inner surface area of described container bottoms, and unit can be set to cm2。
Below by way of a concrete preferred embodiment, the present invention is elaborated.
A kind of at silicone rubber strip quantitatively, the method for periphtic algae equably, comprise the steps:
S1, measure the density of frustule in algae solution: cultured algae solution dropped in and blood counting chamber is made counting load, detailed process is: in the upper count block place covered of blood counting chamber, algae solution is rocked after uniformly, a small amount of algae solution is drawn with glue head dropper, dropping in the dropping liquid groove of blood counting chamber, algae solution will infiltrate count block voluntarily by the effect of liquid tension, and namely load completes.It is then transported to optical microphotograph Microscopic observation counting, with reference to amplification is: 16 times of eyepieces and 40 times of object lens.Adopt the method that this area is conventional, due to counting region volume it is known that the density C (individual/mL, namely frustule quantity contained in every mL algae solution) of frustule in algae solution can be conversed by frustule sum.In this example, adopting the chlorella that BG11 fluid medium is cultivated, calculating the algae cell density obtained in algae solution is 1.5 × 107Individual/ml.The method provided in this step is blood counting chamber method, but is not limited to the method
S2, make the container of certain size as required, and silicone rubber test piece is cut into identical shape by the bottom shape and size according to this container, and silicone rubber plate placement in a reservoir and is abutted in container bottoms, gap between silicone rubber test piece and described container side wall is within 0.5mm, to ensure when quantitatively moving into algae solution, reduce leakage algae amount in space, it is ensured that quantitative accuracy as far as possible.Wherein, as shown in Figure 1, putting into the front elevational schematic after in container 2 for silicone rubber test piece 1, in this example, container is that cylinder (in practice, is not limited to this shape, can also be cube etc.), silicone rubber test piece 1 is circular, and D is the diameter (owing to the space σ between silicone rubber test piece and internal tank is only small, D can also regard the internal diameter of container 2 as) of silicone rubber test piece 1, in this example, D is 10cm；D is the thickness of silicone rubber test piece, is advisable in 1mm-5mm, and in this example, thickness d is 2mm.In this example, it is preferred that the material of container is colourless transparent glass, such algae is not easily attached to container inner wall and is prone to observe the adhesion condition of algae, reduces quantitative error.
S3, according to predetermined silicone rubber strip algae cell density, adds the algae solution of the known algae cell density of (adding for example with pipettor) certain volume in container, and the liquid level of the algae solution of addition is higher than the upper surface of silicone rubber test piece.According to actual needs, predetermined silicone rubber strip algae cell density is to be not less than 2 × 107Individual/cm2It is advisable, by 2 × 10 in this example7Individual/cm2Calculating, the volume of the algae solution of addition calculates with following formula:
Wherein: V represents the volume of the algae of addition；A represents silicone rubber strip algae cell density；C represents the algae cell density in algae solution；S represents the upper surface area of silicone rubber test piece or the inner surface area of described container bottoms.Specific to this example, volume is:
Wherein, D=10cm, A=2 × 107Individual/cm2, C=1.5 × 107Individual/ml, is calculated by data above and obtains V=314mL, namely adds 314mL algae solution in container.
S4, the container that with the addition of algae solution is placed on aeration-drying place, make the upper surface of silicone rubber test piece and liquid level all maintenance levels of algae solution, treat that algae solution is dried, in whole dry run, the upper surface of silicone rubber test piece and the liquid level of algae solution remain level, can obtain quantitatively, be attached to equably the silicone rubber test piece of algae.In this example, predetermined is adhere to 2 × 10 in silicone rubber test piece7Individual/cm2Frustule, taking out after silicone rubber test piece, the chamber wall that makes discovery from observation remaining almost without algae (green), illustrating that the algae solution of addition is substantially all and be attached in silicone rubber test piece, quantitatively accurately and reliably.
Take out this silicone rubber test piece being attached to algae, get final product direct contact angle method and measure the hydrophobicity performance on its surface.By different quantitative requirements, it is possible to obtain the silicone rubber test piece being attached to algae of different basis weights, such that it is able to record the relation of algae adhesion amount and silastic material surface hydrophobicity.
Above content is in conjunction with specific embodiment further description made for the present invention, it is impossible to assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, make some replacements or obvious modification without departing from the inventive concept of the premise, and performance or purposes are identical, all should be considered as belonging to protection scope of the present invention.
Claims (7)
1. one kind at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that comprise the steps:
(1) silicone rubber test piece being put into a container the described container bottoms that reclines, the shape of described silicone rubber test piece is identical with the shape of described container bottoms, and the gap between described silicone rubber test piece and described container side wall is within 0.5mm；
(2) according to predetermined silicone rubber strip algae cell density, adding the algae solution of the known algae cell density of certain volume in described container, the liquid level of the algae solution of addition is higher than the upper surface of described silicone rubber test piece；
(3) upper surface of described silicone rubber test piece and the liquid level of described algae solution are all kept level, treat that described algae solution is dried, obtain being attached to quantitatively, equably the silicone rubber test piece of algae.
2. as claimed in claim 1 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: the volume following formula of the algae added in described step (2) calculates:
Wherein: V represents the volume of the algae of addition；A represents silicone rubber strip algae cell density；C represents the algae cell density in algae solution；S represents the upper surface area of silicone rubber test piece or the inner surface area of described container bottoms.
3. as claimed in claim 1 or 2 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: the thickness of described silicone rubber test piece is 1mm-5mm.
4. as claimed in claim 1 or 2 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: silicone rubber strip algae cell density predetermined in described step (2) is not less than 2 × 107Individual/cm2。
5. as claimed in claim 1 or 2 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: described container is cylinder or cube.
6. as claimed in claim 1 or 2 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: the material of described container is colourless transparent glass.
7. as claimed in claim 1 or 2 at silicone rubber strip quantitatively, the method for periphtic algae equably, it is characterised in that: in described step (2), the algae cell density of algae solution is calculated after being counted by blood counting chamber and obtains.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511000588.1A CN105738171B (en) | 2015-12-28 | 2015-12-28 | A method of silicon rubber strip is quantitative, equably periphtic algae |
| PCT/CN2016/112796 WO2017114437A1 (en) | 2015-12-28 | 2016-12-28 | Method for enabling algae quantitatively and uniformly adhere to surface of silicone rubber test piece |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511000588.1A CN105738171B (en) | 2015-12-28 | 2015-12-28 | A method of silicon rubber strip is quantitative, equably periphtic algae |
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| CN105738171A true CN105738171A (en) | 2016-07-06 |
| CN105738171B CN105738171B (en) | 2019-01-29 |
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| CN201511000588.1A Expired - Fee Related CN105738171B (en) | 2015-12-28 | 2015-12-28 | A method of silicon rubber strip is quantitative, equably periphtic algae |
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| WO (1) | WO2017114437A1 (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489709A (en) * | 2016-10-20 | 2017-03-15 | 清华大学深圳研究生院 | A kind of method for cultivating algae on composite insulating material surface |
| CN106501697A (en) * | 2016-10-20 | 2017-03-15 | 清华大学深圳研究生院 | A kind of environment simulator and method in charged insulating sublist face attached algae |
| WO2017114437A1 (en) * | 2015-12-28 | 2017-07-06 | 清华大学深圳研究生院 | Method for enabling algae quantitatively and uniformly adhere to surface of silicone rubber test piece |
| CN110669664A (en) * | 2019-10-22 | 2020-01-10 | 南方电网科学研究院有限责任公司 | Electric field treatment test method and device for algae on surface of silicone rubber |
Citations (3)
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| CN1793911A (en) * | 2005-12-29 | 2006-06-28 | 中国海洋大学 | Indoor hanging plate method for effectively evaluating resin antifouling performance |
| JP2008011734A (en) * | 2006-07-04 | 2008-01-24 | Miyagi Prefecture | Method for producing seed and seedling of alga of phodophyceae rhodymeniales, and method for culturing alga of phodophyceae rhodymeniales |
| CN104774765A (en) * | 2015-04-09 | 2015-07-15 | 新奥科技发展有限公司 | Cell inoculation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104568676A (en) * | 2015-01-20 | 2015-04-29 | 清华大学深圳研究生院 | Method and device for testing hydrophobic property of material |
| CN105738171B (en) * | 2015-12-28 | 2019-01-29 | 清华大学深圳研究生院 | A method of silicon rubber strip is quantitative, equably periphtic algae |
-
2015
- 2015-12-28 CN CN201511000588.1A patent/CN105738171B/en not_active Expired - Fee Related
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2016
- 2016-12-28 WO PCT/CN2016/112796 patent/WO2017114437A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1793911A (en) * | 2005-12-29 | 2006-06-28 | 中国海洋大学 | Indoor hanging plate method for effectively evaluating resin antifouling performance |
| JP2008011734A (en) * | 2006-07-04 | 2008-01-24 | Miyagi Prefecture | Method for producing seed and seedling of alga of phodophyceae rhodymeniales, and method for culturing alga of phodophyceae rhodymeniales |
| CN104774765A (en) * | 2015-04-09 | 2015-07-15 | 新奥科技发展有限公司 | Cell inoculation method |
Non-Patent Citations (1)
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| 刘红 等: "无毒防污涂料表面底栖硅藻附着评价的实验方法", 《海洋环境科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017114437A1 (en) * | 2015-12-28 | 2017-07-06 | 清华大学深圳研究生院 | Method for enabling algae quantitatively and uniformly adhere to surface of silicone rubber test piece |
| CN106489709A (en) * | 2016-10-20 | 2017-03-15 | 清华大学深圳研究生院 | A kind of method for cultivating algae on composite insulating material surface |
| CN106501697A (en) * | 2016-10-20 | 2017-03-15 | 清华大学深圳研究生院 | A kind of environment simulator and method in charged insulating sublist face attached algae |
| CN110669664A (en) * | 2019-10-22 | 2020-01-10 | 南方电网科学研究院有限责任公司 | Electric field treatment test method and device for algae on surface of silicone rubber |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017114437A1 (en) | 2017-07-06 |
| CN105738171B (en) | 2019-01-29 |
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