CN105733993A - Method for utilizing Fe-C primary battery for deoxidization to culture clostridium butyricum - Google Patents
Method for utilizing Fe-C primary battery for deoxidization to culture clostridium butyricum Download PDFInfo
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Abstract
The invention discloses a method for utilizing an Fe-C primary battery deoxidization principle for rapidly consuming dissolvent oxygen in an anaerobic clostridium butyricum culture solution.Clostridium butyricum belongs to anaerobic bacteria and can grow only in an oxygen-free environment; in order to eliminate the dissolve oxygen in the culture environment, the optimal deoxidant reduced Fe powder and the most appropriate addition amount are obtained from a series of deoxidant through screening, it is confirmed that the reduced Fe powder and C powder form a primary battery to achieve an ideal deoxidization effect to meet the growth conditions of clostridium butyricum, and the biomass of clostridium butyricum is increased to 4.05*108 pieces /ml from 2.04*108 pieces /mL.The method has the advantages that cost is low, operation is easy, and the deoxidization effect is good.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to one and utilize primary iron-graphite cell deoxygenation to cultivate Clostridium butyricum
Method.
Background technology
Clostridium butyricum (Clostridium butyricum), has another name called man in the palace bacterium, clostridium butyricum, and bacteriology's classification belongs to shuttle
Pseudomonas, is the Gram-positive bacillus cereus of a kind of strictly anaerobic, and the Gram’s staining of late stage of culture antibacterial is negative.Mainly deposit
It is in intestinal and the feces of cheese, natural Yoghourt, people and animal, it is possible to be present in the middle of some soil, leaves.Clostridium butyricum
There is stronger environmental resistance, in people with animal body, can be the most permissible with resisting high-concentration gastric acid and bile salt, Digestive system
Harmful levels of pathogens in suppression intestinal, such as escherichia coli, Salmonella etc.；The growth of beneficial bacteria of intestinal tract can also be promoted, such as lactic acid
Bacillus, bacillus bifidus etc..In addition, other Multiple Classes of Antibiotics, only to a few antibiotic sensitive, is had by Clostridium butyricum
The strongest drug resistance.Owing to possessing above feature, Clostridium butyricum is considered as a kind of comparatively ideal to have extensive exploitation prospect
Microbial ecological agent, and extensively application and clinical medicine and animal husbandry aspect.
The definition to probiotic bacteria of the brainstrust according to calendar year 2001 FAO (Food and Agriculture Organization of the United Nation) and World Health Organization (WHO), Clostridium butyricum
Comply fully with the standard of probiotic bacteria.It puts into clinical practice early than nineteen forty-four in Japan, through nearly 70 years as probiotic bacteria
Research, has been developed over multiple relevant microbial ecological agent product, such as drug for controlling intestinal function thing, health food, feed additive, microorganism
Fertilizer etc..Clostridium butyricum is mainly played a role by the following aspects: 1. maintains the microecological balance in animal intestinal, promotes
The propagation of probiotics (lactobacillus, bacillus bifidus) and growth, suppress the harmful bacterias such as harmful bacteria (escherichia coli, Salmonella)
Growth and breeding.2. produce the multiple enzyme to animals useful, vitamin etc., improve the abilities of digestive and absorption of animal.3. produce butanoic acid, promote
Precession thing intestinal epithelial cell tissue regeneration and reparation, maintain the health of intestinal.
Clostridium butyricum belongs to strict anaerobe.Document is reported, trace dissolved oxygen (40uM/L) can suppress it to grow, when
In solution, dissolved oxygen reaches to stop growing completely during 120uM/L.Though having also it has been reported that some Clostridium butyricum also can be detested micro-
Growing under oxygen environment, but the overwhelming majority can only be grown under oxygen-free environment, its suitableeest initial oxidation reduction potential is-21 mV.
What current industrial cultivation produced Clostridium butyricum employing is all repeatedly to be purged method to build anaerobic growth environment by nitrogen, with full
The growth conditions of foot Clostridium butyricum.But the method also has its limitation: 1. operate complexity: will repeatedly lead to nitrogen purging, and can not
Ensure to reach complete anaerobic condition；2. cost is high: nitrogen can not be recycled, and there is also the transport of nitrogen in process of production,
The series of problems such as storage.
In order to find a kind of economical and practical, easily operated cultural method, researchers propose as added chemical deoxidization
A series of schemes such as agent, mixed fungus fermentation cultivation, physics deoxygenation.The invention utilize Fe-C galvanic element to eliminate cultivation
Dissolved oxygen in liquid.Its principle for adding appropriate reduction Fe powder and activity C powder (grinding to form graininess) in culture fluid, Fe powder and
Activity C can form numerous miniature galvanic cell, can accelerate 02Consumption, quickly reduce oxidoreduction electricity in solution with this
Gesture, reacts and is: positive pole: O2+4e+2H2O=4OH-Negative pole: Fe-2e=Fe2+.Its overall reaction equation is 2Fe+02+2H20=2Fe
(OH)2。
Summary of the invention
The technical problem to be solved is to provide the simple deoxygenation side during a kind of liquid culture Clostridium butyricum
Method, overcomes the operation during conventional logical nitrogen deoxygenation is cultivated complicated, the shortcoming that cost is high.
In order to realize the object of the invention, the present inventor finally sets up one by great many of experiments and utilizes Fe and C powder, is formed former
Battery reaches the method for quick deoxygenation, and optimizes the condition of culture of Clostridium butyricum on this basis.
A kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, adds in Clostridium butyricum fermentation initial medium
With oxygen scavenger and C powder, described oxygen scavenger is cysteine hydrochloride, sodium thioglycolate, anhydrous sodium sulfite, thiosulfuric acid
Any one in sodium, ascorbic acid, reduction Fe powder, ferrous sulfate heptahydrate, preferably reduction Fe powder.
Preferably, a kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, it is characterised in that:
The interpolation mass volume ratio of reduction Fe powder and Clostridium butyricum fermentation initial medium is 0.05%, 0.1%, 0.5%, 1.0%, 2.0%,
4.0%, optimum addition 0.5%.
Preferably, a kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, it is characterised in that:
The interpolation mass volume ratio of C powder and Clostridium butyricum fermentation initial medium is 0.05%, 0.1%, 0.3%, 0.6%, 0.9%, 1.2%,
1.5%, optimum addition is 0.3%.
Preferably, described Clostridium butyricum fermentation initial medium component is: glucose 10.0g/L, peptone 10.0g/
L, tryptone 10.0g/L, soy peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate
2.5g/L、PH7.0±0.1。
Preferably, described Clostridium butyricum activation medium (RCM) component is: glucose 10.0g/L, peptone 10.0g/
L, tryptone 10.0g/L, soy peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate
2.5g/L, sodium thioglycolate 0.3g/L, cysteine hydrochloride 0.3g/L, PH7.0 ± 0.1.
Preferably, described butyrate spindle bacillus seed media components is: glucose 10.0g/L, peptone 10.0g/L, pancreas
Peptone 10.0g/L, soy peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L,
PH7.0±0.1。
The present invention compared with prior art, possesses advantages below and effect: utilize Fe-C galvanic element can accelerate to disappear oxygen
The principle of consumption, well solves the deoxygenation problem in strict anaerobes incubation, and traditional by repeatedly leading to a large amount of nitrogen
Gas is built anaerobic environment method and is compared, and low cost is simple to operate, and deaerating effect is good.
Accompanying drawing explanation
Fig. 1 is Clostridium butyricum growth curve chart.
Fig. 2 is that different oxygen scavenger affects bar diagram to Biomass.
Fig. 3 is that Fe powder addition optimizes bar diagram.
Fig. 4 is that C powder addition optimizes bar diagram.
Detailed description of the invention
The following is the specific embodiment of the present invention, technical scheme is described further, but the present invention's is interior
Hold the scope being not limited solely to described in embodiment, every change without departing substantially from present inventive concept or equivalent replacement and be included in this
Within the protection domain of invention.
Embodiment 1
Actication of culture: cold preservation strain in 20 DEG C of glycerol pipes is inoculated into 18 × 180mm equipped with 10mLRCM culture fluid and detests
In oxygen pipe, overlying 1cm liquid paraffin, culture medium shifts to an earlier date sterilizing, and cultivation temperature is 37 DEG C.
Activation medium (RCM) component is: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, big
Soybean protein peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, sodium thioglycolate 0.3g/
L, cysteine hydrochloride 0.3g/L, PH7.0 ± 0.1.
Embodiment 2
Seed culture and growth curve are drawn: the bacterium solution after activation is inoculated into the 18 × 180mm anaerobism equipped with seed culture fluid
Guan Zhong, totally 50, and under spectrophotometer 600nm wavelength, start to survey every 2h the light absorption value of its seed liquor from 0h, survey every time
3 parallel, cultivation temperature 37 DEG C, finally draws out the seed growth curve (Fig. 1) of Clostridium butyricum.
Seed culture medium component is: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, soybean protein
Peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, PH7.0 ± 0.1.
Embodiment 3
The suitableeest oxygen scavenger screens: oxygen scavenger is at cysteine hydrochloride, sodium thioglycolate, anhydrous sodium sulfite, thiosulfuric acid
Screening in sodium, ascorbic acid, reduction Fe powder, ferrous sulfate heptahydrate, fixing Preliminary fermentation Media Components is constant, every kind of oxygen scavenger
Addition is 1.0g/L.Shown by Fig. 1 result and choose 7h as inoculation time, inoculum concentration 1.0g/L, liquid amount 100ml
(250ml triangular flask), cultivation temperature 37 DEG C, use blood counting chamber to survey its thalline number after cultivating 18h, each sample arranges 2
Parallel, oxygen scavenger the selection result is shown in Fig. 2.Fe powder best results as shown in Figure 2, than blank (without any oxygen scavenger)
Biomass is by 2.04 × 108Individual/mL rises to 2.69 × 108Individual/mL, so choosing Fe powder as oxygen scavenger.
Preliminary fermentation media components: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, Semen sojae atricolor egg
White peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, PH7.0 ± 0.1.
Embodiment 4
Oxygen scavenger addition (w/v) optimizes: selects Fe powder as optimal oxygen scavenger as shown in Figure 2, optimizes Fe powder on this basis
Addition, choose 0.05%, 0.1%, 0.5%, 1.0%, 2.0%, the mass volume ratio of 4.0%(oxygen scavenger and fermentation medium) six
Concentraton gradient, other composition of solid-state fermentation initial medium is constant, 37 DEG C, uses blood counting chamber to survey its thalline after cultivating 18h
Number, each sample arrange 2 parallel, addition effect of optimization is shown in Fig. 3.As shown in Figure 3 when addition is 0.5%, Clostridium butyricum
Biomass reaches to be 3.12 × 10 to the maximum8Individual/mL.
Preliminary fermentation media components: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, Semen sojae atricolor egg
White peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, PH7.0 ± 0.1.
Embodiment 5
C powder addition (w/v) optimizes: chooses the 0.5% powder optimum addition of Fe the most as shown in Figure 3, optimizes C powder on this basis
Addition, it is fixing that to optimize other composition of post-fermentation and culture base constant, add 0.05% respectively, 0.1%, 0.3%, 0.6%, 0.9%,
1.2%, the mass volume ratio of 1.5%(C powder and fermentation medium) C powder, 37 DEG C, after cultivating 18h, use blood counting chamber to survey it
Thalline number, each sample arrange 2 parallel, the best results when C powder addition is 0.3% as shown in Figure 4, than blank (be not added with
C powder), cell concentration is by 3.15 × 108Individual/mL rises to 4.05 × 108Individual/mL.
Optimization basis set part of post-fermentation and culture: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, Semen sojae atricolor
Peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L, Fe powder 5g/L, PH7.0 ±
0.1。
Claims (5)
1. one kind utilizes the method that Clostridium butyricum is cultivated in Fe-C galvanic element deoxygenation, it is characterised in that: initial in Clostridium butyricum fermentation
Adding oxygen scavenger and C powder in culture medium, described oxygen scavenger is L cysteine hydrochloride, sodium thioglycolate, anhydrous sulfurous
Any one in acid sodium, sodium thiosulfate, ascorbic acid, reduction Fe powder, ferrous sulfate heptahydrate.
A kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, it is characterised in that: remove
Fe powder is preferably reduced in oxygen agent.
A kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, its feature exists
In: the interpolation mass volume ratio of reduction Fe powder and Clostridium butyricum fermentation initial medium be 0.05%, 0.1%, 0.5%,
1.0%, 2.0%, 4.0%, optimum addition 0.5%.
A kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, it is characterised in that: C
The interpolation mass volume ratio of powder and Clostridium butyricum fermentation initial medium is 0.05%, 0.1%, 0.3%, 0.6%, 0.9%,
1.2%, 1.5%, optimum addition is 0.3%.
A kind of method utilizing Fe-C galvanic element deoxygenation to cultivate Clostridium butyricum, it is characterised in that: fourth
Acid clostridial fermentation initial medium component is: glucose 10.0g/L, peptone 10.0g/L, tryptone 10.0g/L, Semen sojae atricolor egg
White peptone 5.0g/L, yeast leaching powder 3.0g/L, sodium chloride 3.0g/L, dipotassium hydrogen phosphate 2.5g/L.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109825459A (en) * | 2019-03-29 | 2019-05-31 | 天津科技大学 | One plant of coupling produces the dissimilatory iron reduction bacterium of hydrogen |
| CN113736698A (en) * | 2021-09-02 | 2021-12-03 | 江南大学 | Optimization of clostridium butyricum amplification culture medium |
| CN115261274A (en) * | 2022-08-05 | 2022-11-01 | 天康制药(苏州)有限公司 | High-density fermentation medium for staphylococcus epidermidis and fermentation method and application thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515471A (en) * | 2003-01-06 | 2004-07-28 | 北京东方百信生物技术有限公司 | Room temp. storage method of product containing anaerobic formers and product packaged by adopting said method |
| JP2007319151A (en) * | 2006-06-02 | 2007-12-13 | Chitsuyoshi Kawaguchi | Fermented soybean paste and soy sauce added with clostridium butyricum |
| CN101397546A (en) * | 2008-10-17 | 2009-04-01 | 杭州百思生物技术有限公司 | Quick-speed generation method of anaerobic microorganism culture environment |
| CN102363754A (en) * | 2011-05-07 | 2012-02-29 | 华中农业大学 | Clear liquid fermentation medium for clostridium butyricum and fermentation culture method thereof |
| CN102965323A (en) * | 2012-12-24 | 2013-03-13 | 厦门大学 | Screening method and application of strain for producing 1,3-propanediol |
| CN103951016A (en) * | 2014-04-28 | 2014-07-30 | 上海大学 | Method for treating nitrogenous wastewater by using iron-carbon complexing agent |
| CN105360874A (en) * | 2014-08-21 | 2016-03-02 | 武汉凌科达科技有限公司 | Deoxidizer for food fresh keeping |
-
2016
- 2016-04-05 CN CN201610207538.9A patent/CN105733993B/en not_active Expired - Fee Related
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1515471A (en) * | 2003-01-06 | 2004-07-28 | 北京东方百信生物技术有限公司 | Room temp. storage method of product containing anaerobic formers and product packaged by adopting said method |
| JP2007319151A (en) * | 2006-06-02 | 2007-12-13 | Chitsuyoshi Kawaguchi | Fermented soybean paste and soy sauce added with clostridium butyricum |
| CN101397546A (en) * | 2008-10-17 | 2009-04-01 | 杭州百思生物技术有限公司 | Quick-speed generation method of anaerobic microorganism culture environment |
| CN102363754A (en) * | 2011-05-07 | 2012-02-29 | 华中农业大学 | Clear liquid fermentation medium for clostridium butyricum and fermentation culture method thereof |
| CN102965323A (en) * | 2012-12-24 | 2013-03-13 | 厦门大学 | Screening method and application of strain for producing 1,3-propanediol |
| CN103951016A (en) * | 2014-04-28 | 2014-07-30 | 上海大学 | Method for treating nitrogenous wastewater by using iron-carbon complexing agent |
| CN105360874A (en) * | 2014-08-21 | 2016-03-02 | 武汉凌科达科技有限公司 | Deoxidizer for food fresh keeping |
Non-Patent Citations (2)
| Title |
|---|
| HEGUANG ZHU等: "Effect of ferrous ion on photo heterotrophic hydrogen production by Rhodobacter sphaeroides", 《INTERNATIONAL JOURNAL OF HYDROGEN ENERGY》 * |
| 戚薇等: "酪酸梭状芽孢杆菌发酵培养基的优化", 《天津科技大学学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109825459A (en) * | 2019-03-29 | 2019-05-31 | 天津科技大学 | One plant of coupling produces the dissimilatory iron reduction bacterium of hydrogen |
| CN109825459B (en) * | 2019-03-29 | 2021-09-14 | 天津科技大学 | Dissimilatory iron reduction bacterium for coupling hydrogen production |
| CN113736698A (en) * | 2021-09-02 | 2021-12-03 | 江南大学 | Optimization of clostridium butyricum amplification culture medium |
| CN113736698B (en) * | 2021-09-02 | 2024-03-01 | 江南大学 | Optimization of clostridium butyricum expansion medium |
| CN115261274A (en) * | 2022-08-05 | 2022-11-01 | 天康制药(苏州)有限公司 | High-density fermentation medium for staphylococcus epidermidis and fermentation method and application thereof |
| CN115261274B (en) * | 2022-08-05 | 2023-11-21 | 天康制药股份有限公司 | High-density fermentation medium for staphylococcus epidermidis as well as fermentation method and application of high-density fermentation medium |
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