CN105728309A - Method for purifying pollen for fruit tree pollination - Google Patents

Method for purifying pollen for fruit tree pollination Download PDF

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Publication number
CN105728309A
CN105728309A CN201610076570.8A CN201610076570A CN105728309A CN 105728309 A CN105728309 A CN 105728309A CN 201610076570 A CN201610076570 A CN 201610076570A CN 105728309 A CN105728309 A CN 105728309A
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CN
China
Prior art keywords
pollen
acetone
sieve
container
fruit tree
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610076570.8A
Other languages
Chinese (zh)
Inventor
赵奎
马建江
牛自勉
杨方
孙俊宝
潘大丰
姚瑞
赵莲
畅文选
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pinglu Jintong Fruit Industry Specialized Cooperative
Original Assignee
Pinglu Jintong Fruit Industry Specialized Cooperative
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pinglu Jintong Fruit Industry Specialized Cooperative filed Critical Pinglu Jintong Fruit Industry Specialized Cooperative
Priority to CN201610076570.8A priority Critical patent/CN105728309A/en
Publication of CN105728309A publication Critical patent/CN105728309A/en
Pending legal-status Critical Current

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B07SEPARATING SOLIDS FROM SOLIDS; SORTING
    • B07BSEPARATING SOLIDS FROM SOLIDS BY SIEVING, SCREENING, SIFTING OR BY USING GAS CURRENTS; SEPARATING BY OTHER DRY METHODS APPLICABLE TO BULK MATERIAL, e.g. LOOSE ARTICLES FIT TO BE HANDLED LIKE BULK MATERIAL
    • B07B1/00Sieving, screening, sifting, or sorting solid materials using networks, gratings, grids, or the like

Abstract

The invention relates to a method for purifying pollen for fruit tree pollination, and belongs to the technical field of pollen purification. The technical problems that according to pollen screened and extracted by an existing sieve, the amount of fluff containing torus is large, a pollination mechanical channel is prone to being blocked, the pollen utilization rate is low, and the effect is poor are mainly solved. To solve the above technical problems, according to the adopted technical scheme, the method for purifying the pollen for fruit tree pollination comprises the following steps that firstly, primarily-extracted pollen is contained in a sieve with 120 meshes under the conditions that ventilation is good, and the temperature ranges from 0 DEG C to 20 DEG C; and secondly, the sieve containing the primarily-extracted pollen is placed in a container containing an acetone solution, the sieve is shaken, the pollen is made to be deposited to the bottom of the acetone solution, and after the pollen is deposited, the redundant acetone solution is discharged, then, the pollen deposited at the bottom of the container is taken out and contained in a container with good heat conductivity, the acetone volatilizes for 15 min-30 min, and after the acetone is completely volatized, the purified pollen is taken out, collected and packaged.

Description

A kind of method of purification of Fruit Tree Pollination pollen
Technical field
The present invention relates to the method for purification of a kind of Fruit Tree Pollination pollen, it belongs to the technical field purifying pollen.
Background technology
Fruit tree artificial pollination can make up the impact that fruit fertilization is caused by florescence bad climate factor, and stable orchard yield and raising fruit quality are had a very important role.Along with the progressively universal of fruit tree artificial pollination technology and human cost improve, Mechanical pollination is commonly used at world's every country.
Owing to Mechanical pollination is more and more higher to the purity requirement of pollen, the pollen that the conventional sieve of tradition proposes often contain the fine hair of holder, can block the passage that pollination is mechanical in pollinating process, and how extracting highly purified pollen is problem in the urgent need to address.
Summary of the invention
Present invention aim to address the pollen that existing sieve proposes also exist the fine hair containing holder many, easily block pollination machinery passage, pollen utilization rate is low and the technical problem of weak effect, it is provided that the method for purification of a kind of Fruit Tree Pollination pollen.
For solving above-mentioned technical problem, the technical solution used in the present invention is:
A kind of method of purification of Fruit Tree Pollination pollen, it is made up of following step:
1) when well-ventilated's temperature is 0~20 DEG C, first jacquard weave powder is placed in the sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good container of heat conduction and allows acetone volatilize 15~30 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
The weight ratio of described just jacquard weave powder and acetone soln is 12.
Owing to present invention employs technique scheme, acetone is utilized to purify pollen, it is possible to being completely separated out in first jacquard weave powder the stickums such as the fine hair mixed, and the pectin on solubilized pollen surface, the dispersibility making pollen is better, improves the utilization rate of pollen.Solve existing sieve propose pollen also exist the fine hair containing holder many, easily block pollination machinery passage technology problem.Therefore, compared with background technology, the present invention has the pollen of extraction without fine hair impurity, the high and effective advantage of utilization rate that will not block pollination machinery passage, pollen.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1
A kind of method of purification of Fruit Tree Pollination pollen, it is made up of following step:
1) when well-ventilated's temperature is 0~20 DEG C, first jacquard weave powder is placed in the rustless steel sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good aluminium vessel of heat conduction and allows acetone volatilize 15~30 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
The weight ratio of described just jacquard weave powder and acetone soln is 12.Acetone soln is the excellent high-purity acetone that Yanshan Petrochemical produces.
Embodiment 2
A kind of method of purification of Fruit Tree Pollination pollen, it is made up of following step:
1) when well-ventilated's temperature is 20 DEG C, first jacquard weave powder is placed in the rustless steel sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good aluminium vessel of heat conduction and allows acetone volatilize 15 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
The weight ratio of described just jacquard weave powder and acetone soln is 12.Acetone soln is the excellent high-purity acetone that Yanshan Petrochemical produces.
Embodiment 3
A kind of method of purification of Fruit Tree Pollination pollen, it is made up of following step:
1) when well-ventilated's temperature is 15 DEG C, first jacquard weave powder is placed in the rustless steel sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good aluminium vessel of heat conduction and allows acetone volatilize 20 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
The weight ratio of described just jacquard weave powder and acetone soln is 12.Acetone soln is the excellent high-purity acetone that Yanshan Petrochemical produces.
Embodiment 4
A kind of method of purification of Fruit Tree Pollination pollen, it is made up of following step:
1) when well-ventilated's temperature is 0 DEG C, first jacquard weave powder is placed in the rustless steel sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good aluminium vessel of heat conduction and allows acetone volatilize 30 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
The weight ratio of described just jacquard weave powder and acetone soln is 12.Acetone soln is the excellent high-purity acetone that Yanshan Petrochemical produces.

Claims (2)

1. the method for purification of a Fruit Tree Pollination pollen, it is characterised in that: it is made up of following step:
1) when well-ventilated's temperature is 0~20 DEG C, first jacquard weave powder is placed in the sieve of 120 orders;
2) sieve filling just jacquard weave powder is put into equipped with in the container of acetone soln, shake sieve, pollen is made to sink at the bottom of acetone soln, after pollen precipitation, discharge unnecessary acetone soln, then the pollen being deposited in container bottom is taken out, it is placed in the good container of heat conduction and allows acetone volatilize 15~30 minutes, after acetone volatilizees completely, take out and purify pollen, collecting packing.
2. the method for purification of Fruit Tree Pollination pollen according to claim 1, it is characterised in that: the weight ratio of described just jacquard weave powder and acetone soln is 12.
CN201610076570.8A 2016-02-03 2016-02-03 Method for purifying pollen for fruit tree pollination Pending CN105728309A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610076570.8A CN105728309A (en) 2016-02-03 2016-02-03 Method for purifying pollen for fruit tree pollination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610076570.8A CN105728309A (en) 2016-02-03 2016-02-03 Method for purifying pollen for fruit tree pollination

Publications (1)

Publication Number Publication Date
CN105728309A true CN105728309A (en) 2016-07-06

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CN (1) CN105728309A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107674856A (en) * 2017-09-14 2018-02-09 漳州卫生职业学院 A kind of separation method of amomum viosum Generative Cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2042603A1 (en) * 1998-11-17 2009-04-01 Monsanto Technology, LLC Phosphonate metabolizing plants
CN101617619A (en) * 2009-07-31 2010-01-06 中国农业大学 Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof
CN103114120A (en) * 2011-11-16 2013-05-22 中国科学院上海生命科学研究院 Carotenoid synthesis related gene and its application
CN103293212A (en) * 2013-06-06 2013-09-11 安徽徽大农业有限公司 Method for checking purity of hybridized pepper seeds

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2042603A1 (en) * 1998-11-17 2009-04-01 Monsanto Technology, LLC Phosphonate metabolizing plants
CN101617619A (en) * 2009-07-31 2010-01-06 中国农业大学 Pollen suspension suitable for liquid pollination of fruit trees and preparation method thereof
CN103114120A (en) * 2011-11-16 2013-05-22 中国科学院上海生命科学研究院 Carotenoid synthesis related gene and its application
CN103293212A (en) * 2013-06-06 2013-09-11 安徽徽大农业有限公司 Method for checking purity of hybridized pepper seeds

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107674856A (en) * 2017-09-14 2018-02-09 漳州卫生职业学院 A kind of separation method of amomum viosum Generative Cells
CN107674856B (en) * 2017-09-14 2020-06-26 漳州卫生职业学院 Method for separating amomum villosum pollen germ cells

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Application publication date: 20160706

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