CN105726783A - Composition with antibacterial function as well as preparation method and application thereof - Google Patents

Composition with antibacterial function as well as preparation method and application thereof Download PDF

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Publication number
CN105726783A
CN105726783A CN201610065041.8A CN201610065041A CN105726783A CN 105726783 A CN105726783 A CN 105726783A CN 201610065041 A CN201610065041 A CN 201610065041A CN 105726783 A CN105726783 A CN 105726783A
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China
Prior art keywords
weight portion
solution
antibacterial
compositions
hemostasis
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CN201610065041.8A
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Chinese (zh)
Inventor
刘建顺
黄进明
洪绯
林小红
潘裕添
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Zhangzhou Pientzehuang Pharmaceutical Co Ltd
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Zhangzhou Pientzehuang Pharmaceutical Co Ltd
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Priority to CN201610065041.8A priority Critical patent/CN105726783A/en
Publication of CN105726783A publication Critical patent/CN105726783A/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
    • A61K31/19—Carboxylic acids, e.g. valproic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A61K31/716—Glucans
    • A61K31/722—Chitin, chitosan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/22—Heterocyclic compounds, e.g. ascorbic acid, tocopherol or pyrrolidones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
    • A61K9/006—Oral mucosa, e.g. mucoadhesive forms, sublingual droplets; Buccal patches or films; Buccal sprays
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions

Abstract

The invention relates to a composition with antibacterial, hemostasis and anti-inflammation functions as well as a preparation method and application thereof. The composition comprises the following raw materials in parts by weight: 2-10 parts of chitosan, 50-150 parts of sorbitol, 10-100 parts of glycerin, 0.1-1.0 part of vitamin C, 0.05-0.50 part of potassium sorbate, 1-5 parts of Baiyaqilan extract and 100-500 parts of purified water. In the invention, through optimization selection of the composition and proportion of the components, the components mutually cooperate and act together according to the proportion to realize the antibacterial, hemostasis and anti-inflammation functions. The mouth rinse prepared from the composition forms a protective film on the surface of oral mucosa, and the wound is physically covered to control the uncomfortable symptoms caused by oral ulcer, periodontitis, sphagitis, oral malodor cleaning and the like; and moreover, compared with other mouth rinse, the in-vitro cell toxicity is lower, the safety is higher, and the mouth rinse prepared from the composition is more suitable for long-term use.

Description

A kind of compositions with bacteriostasis and preparation method thereof and purposes
Technical field
The invention belongs to field of oral care, be specifically related to one and there is antibacterial, hemostasis and antiinflammation Compositions and preparation method thereof and purposes.
Background technology
Along with the raising of people's living standard, oral hygiene is increasingly subject to people's attention with health, Especially there is antibacterial or bactericidal action collutory increasingly obtain widely in clinic auxiliary treatment Application.This product can be used on mouth, pharynx, the various illness in chamber and toothwash, have tooth pulled out, have a denture made, orthodontic, During chemicotherapy, perioperatively and the sterilizing oral of bed patient prevent when cross infection.
Chinese patent literature CN104644478A discloses a kind of antibacterial type with nursing repair Collutory, described collutory composition and mass percentage content are as follows: the antibacterial of 0.2-2%, 0.01- Composition, the sweeting agent of 2-5%, the wetting agent of 1-5%, the essence of 0.01-0.1% are repaired in the nursing of 0.2%, Surplus is deionized water；Antibacterial uses chlorhexidine gluconate, chlorhexidine acetate, polyhexamethylene One or both in biguanide；It is in chitosan analog derivative and Citrin that composition is repaired in nursing Plant or two kinds；Chitosan analog derivative is carboxymethyl chitosan, double carboxymethyl chitosan, water soluble shells One or more of polysaccharide；Sweeting agent is the one in xylitol, Sorbitol, aspartame or two Kind；Wetting agent is glycerol or sorbitol.Essence uses water solublity Oleum menthae or green tea extract.So And, owing to above-mentioned collutory uses chlorhexidine gluconate, chlorhexidine acetate, poly hexamethylene biguanide One or both as antibacterial, life-time service be easily caused tooth stain deposition, thus limit its should With.
Therefore, study that novel therapeutic effect is good, toxic and side effects is little has antibacterial, hemostasis and antiinflammatory The collutory of effect is significant.
Summary of the invention
Therefore, the present invention provides that a kind of therapeutic effect is good, toxic and side effects is little have antibacterial, hemostasis and The compositions of antiinflammation, and then its preparation method and purposes are provided.
For solving above-mentioned technical problem, the present invention is achieved through the following technical solutions:
The present invention provides a kind of and has antibacterial, hemostasis and the compositions of antiinflammation, described compositions Raw material includes:
Chitosan 2-10 weight portion, sorbitol 50-150 weight portion, glycerol 10-100 weight portion, dimension Raw element C 0.1-1.0 weight portion, potassium sorbate 0.05-0.50 weight portion, Bai Yaqilan extract 1-5 weight Amount part, pure water 100-500 weight portion.
Preferably, in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention, described group The raw material of compound includes:
Chitosan 4-8 weight portion, sorbitol 60-100 weight portion, glycerol 20-60 weight portion, dimension are raw Element C 0.2-0.6 weight portion, potassium sorbate 0.10-0.20 weight portion, Bai Yaqilan extract 2-4 weight Part, pure water 120-300 weight portion.
It is further preferred that in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention,
The raw material of described compositions also includes pigment, and described pigment is selected from sunset yellow, lemon yellow, light blue In at least one.
It is further preferred that in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention,
Described sunset yellow is 0-0.4 weight portion, and described lemon yellow is 0-0.2 weight portion, and described light blue is 0-0.2 weight portion.
It is further preferred that in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention, Described chitosan is edible fungi chitosan.
It is further preferred that in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention, The very blue extract of described white bud is prepared from by following preparation method: take Bai Yaqilan 90～110 weight Part, heating and refluxing extraction 1～3 times, add 90～110 parts by volume water extraction 1～3h every time, merging carries Take liquid, be concentrated into 9～11 parts by volume, be dried, to obtain final product；
Described weight portion is g/mL with the relation of parts by volume.
It is further preferred that in the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention, The very blue extract of described white bud is prepared from by following preparation method: take Bai Yaqilan 100 weight portion, Heating and refluxing extraction 2 times, adds 100 parts by volume water extraction 2h, united extraction liquid every time, is concentrated into 10 parts by volume, are dried, to obtain final product.
The present invention also provides for the preparation side of a kind of above-mentioned compositions with antibacterial, hemostasis and antiinflammation Method, comprises the following steps:
(1) taking the chitosan of selected weight portion, adding appropriate volume percent is 0.2%～0.4% Acetic acid solution, is configured to the chitosan solution that percetage by weight is 0.5%～1.5%, and stirring is extremely dissolved, Adjusting pH is 5.0～6.0, obtains solution A, standby；
(2) take the pure water of selected weight portion, add vitamin C and the sorbic acid of selected weight portion Potassium, stirring, to dissolving, is then sequentially added into the sorbitol of selected weight portion, glycerol and Bai Ya under stirring Strange blue extract, stirs, obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH is 5.0～6.0, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
Preferably, the preparation method of the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention In, comprise the following steps:
(1) take the chitosan of selected weight portion, add the acetic acid that appropriate volume percent is 0.3% molten Liquid, is configured to the chitosan solution that percetage by weight is 1.0%, and stirring is to dissolving, and adjusting pH is 5.5～5.7, obtain solution A, standby；
(2) take the pure water of selected weight portion, add vitamin C and the sorbic acid of selected weight portion Potassium, stirring, to dissolving, is then sequentially added into the sorbitol of selected weight portion, glycerol and Bai Ya under stirring Strange blue extract, stirs, obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
It is further preferred that the system of the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention In Preparation Method,
In described step (2), adding vitamin C and the step of potassium sorbate of selected weight portion Before, also include the step adding the pigment of selected weight portion.
It is further preferred that the system of the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention In Preparation Method, in described step (1) and/or described step (3), with 0.5～1.5mol/L NaOH solution or the HCl solution adjustment pH of 0.5～1.5mol/L.
It is further preferred that the system of the above-mentioned compositions with antibacterial, hemostasis and antiinflammation of the present invention In Preparation Method, in described step (1) and/or described step (3), molten with the NaOH of 1mol/L The HCl solution of liquid or 1mol/L adjusts pH.
The present invention also provide for including the above-mentioned compositions with antibacterial, hemostasis and antiinflammation preparation, Or including the preparation of the compositions that above-mentioned preparation method prepares, described compositions adds conventional auxiliary Material, according to common process, makes the most acceptable liquid preparation.
Preferably, in the preparation of above-mentioned composition of the present invention, described liquid preparation is oral cavity gargle.
The compositions or above-mentioned that the present invention also provides for above-mentioned composition, above-mentioned preparation method prepares The application in the oral care product that preparation has antibacterial, hemostasis and antiinflammation of the preparation of compositions.
Technical scheme has the advantage that
The present invention have antibacterial, hemostasis and the compositions of antiinflammation, by optimized choice its composition and The proportioning of each component, each component cooperates according to its proportioning, jointly acts on, thus play antibacterial, Hemostasis and antiinflammation.The oral cavity gargle that above-mentioned composition is made is by being formed in oral mucosal surface Protecting film, physics covers wound, reaches anti-treating dental ulcer, periodontitis, pharyngolaryngitis, cleaning oral cavity The malaise symptoms that abnormal flavour etc. cause；And, compared with other oral cavity gargles, its vitro cytotoxicity Lower, safety is higher, is more suitable for life-time service.
Accompanying drawing explanation
In order to be illustrated more clearly that the specific embodiment of the invention or technical scheme of the prior art, under The accompanying drawing used required in detailed description of the invention or description of the prior art will be briefly described by face. It should be evident that the accompanying drawing in describing below is some embodiments of the present invention, general for this area From the point of view of logical technical staff, on the premise of not paying creative work, it is also possible to obtain according to these accompanying drawings Obtain other accompanying drawing.
Fig. 1 is each sample growth inhibition ratio to L929 cell in plate A in experimental example 2 of the present invention；
Fig. 2 is each sample growth inhibition ratio to L929 cell in plate B in experimental example 2 of the present invention.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme is clearly and completely described, it is clear that Described embodiment is a part of embodiment of the present invention rather than whole embodiments.Based on this Embodiment in bright, those of ordinary skill in the art are obtained under not making creative work premise Every other embodiment, broadly fall into the scope of protection of the invention.
In following example of the present invention and experimental example, described chitosan is edible fungi chitosan, purchased from section Lay grace chemical industry (Chinese) company limited；The very blue extract of described white bud prepared by following preparation method and Become: take Bai Yaqilan 100g, heating and refluxing extraction 2 times, add 100mL water extraction 2h every time, United extraction liquid, is evaporated to 10 parts by volume, is dried, to obtain final product；Remaining raw material, reagent and molten Agent is commercially available product.
Embodiment 1
The raw material of the compositions that the present embodiment has antibacterial, hemostasis and antiinflammation consists of: chitosan 4g, sorbitol 80g, glycerol 40g, vitamin C 0.4g, Bai Yaqilan extract 2g, sorbic acid Potassium 0.16g, pure water 160g.
The preparation method of said composition, comprises the following steps:
(1) taking the chitosan of selected weight, adding appropriate volume percent is the acetic acid solution of 0.3%, Being configured to the chitosan solution that percetage by weight is 1.0%, stirring is to dissolving, with 1mol/L's It is 5.5～5.7 that NaOH solution adjusts pH, obtains solution A, standby；
(2) take the pure water of selected weight, add vitamin C and the potassium sorbate of selected weight, Stirring, to dissolving, is then sequentially added into the sorbitol of selected weight, glycerol and Bai Yaqilan under stirring and carries Take thing, stir, obtain solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH by the NaOH solution of 1mol/L is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
The present embodiment has the compositions of antibacterial, hemostasis and antiinflammation and adds customary adjuvant, according to often Rule technique, makes oral cavity gargle.
Embodiment 2
The raw material of the compositions that the present embodiment has antibacterial, hemostasis and antiinflammation consists of:
Chitosan 5g, sorbitol 60g, glycerol 60g, vitamin C 0.2g, Bai Yaqilan extract 3g, light blue 0.05g, potassium sorbate 0.20g, pure water 300g.
The preparation method of said composition, comprises the following steps:
(1) taking the chitosan of selected weight, adding appropriate volume percent is the acetic acid solution of 0.2%, Being configured to the chitosan solution that percetage by weight is 1.5%, stirring is to dissolving, with the HCl of 1mol/L It is 5.5～5.7 that solution adjusts pH, obtains solution A, standby；
(2) take the pure water of selected weight, add the light blue of selected weight, add selected weight Vitamin C and potassium sorbate, stirring, to dissolving, is then sequentially added into the Pyrusussuriensis of selected weight under stirring Alcohol, glycerol and Bai Yaqilan extract, stir, and obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH with the HCl solution of 1mol/L is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
The present embodiment has the compositions of antibacterial, hemostasis and antiinflammation and adds customary adjuvant, according to often Rule technique, makes oral cavity gargle.
Embodiment 3
The raw material of the compositions that the present embodiment has antibacterial, hemostasis and antiinflammation consists of: chitosan 6g, sorbitol 100g, glycerol 20g, vitamin C 0.6g, Bai Yaqilan extract 4g, lemon yellow 0.05g, potassium sorbate 0.10g, pure water 120g.
The preparation method of said composition, comprises the following steps:
(1) taking the chitosan of selected weight, adding appropriate volume percent is the acetic acid solution of 0.4%, Being configured to the chitosan solution that percetage by weight is 0.8%, stirring is to dissolving, with 0.5mol/L's It is 5.5～5.7 that NaOH solution adjusts pH, obtains solution A, standby；
(2) take the pure water of selected weight, add the lemon yellow of selected weight, add selected weight Vitamin C and potassium sorbate, stirring, to dissolving, is then sequentially added into the mountain of selected weight under stirring Pears alcohol, glycerol and Bai Yaqilan extract, stir, and obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH by the NaOH solution of 0.5mol/L is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
The present embodiment has the compositions of antibacterial, hemostasis and antiinflammation and adds customary adjuvant, according to often Rule technique, makes oral cavity gargle.
Embodiment 4
The raw material of the compositions that the present embodiment has antibacterial, hemostasis and antiinflammation consists of:
Chitosan 8g, sorbitol 80g, glycerol 40g, vitamin C 0.4g, Bai Yaqilan extract 4g, sunset yellow 0.2g, potassium sorbate 0.16g, pure water 280g.The preparation method of said composition, Comprise the following steps:
(1) taking the chitosan of selected weight, adding appropriate volume percent is the acetic acid solution of 0.3%, Being configured to the chitosan solution that percetage by weight is 1.2%, stirring is to dissolving, with the HCl of 1.5mol/L It is 5.5～5.7 that solution adjusts pH, obtains solution A, standby；
(2) take the pure water of selected weight, add the sunset yellow of selected weight, add selected weight Vitamin C and potassium sorbate, stirring, to dissolving, is then sequentially added into the mountain of selected weight under stirring Pears alcohol, glycerol and Bai Yaqilan extract, stir, and obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH with the HCl solution of 1.5mol/L is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
The present embodiment has the compositions of antibacterial, hemostasis and antiinflammation and adds customary adjuvant, according to often Rule technique, makes oral cavity gargle.
Experimental example
Experimental example 1The present composition is antibacterial, hemostasis and the evaluation of anti-inflammatory effect
1, experimental technique
1.1 laboratory sample
The oral cavity gargle that experimental group is prepared with embodiment 1 as test sample, matched group 1 group with Listerine collutory is test sample, and matched group 1 group is gargled as test sample with clear water.
1.2 experimental implementation
30 experimenters are chosen: the age, in the range of 20-40 year, is in a good state of health according to following standard, Full mouth must not be less than 20 function teeth, does not uses antibiotic, without gingivitis, tooth in testing first 1 month Zhou Yan, oral mucositis, tested tooth without dental caries damage, abnormal development, gingival atrophy, facing and root face without Charges.
30 experimenters are randomly divided into 3 groups, often organize 10 people, respectively experimental group, matched group 1 and combine Matched group 2 groups.
Normally take food after subject oral cavity cleaning, after 8 hours, start experiment.Experimenter first uses disinfecting silk or cotton Label dip appropriate dental plaque display liquid and are coated in gently on tested facing and carry out dental plaque dyeing, then with clear Water is gargled 3 times, records PLI value, then uses collutory about 20mL rinsing the mouth 40s, with clear after rinsing the mouth Water is gargled 3 times, records PLI value.
Recipient site: #11, #16, #26, #31 buccal labial surface, #36, #46 lingual surface.
2, experimental result
2.1 evaluation criterion
Dye to by inspection facing by 2% Basic Fuchsin in Aqueous Solution, use improvement QiugleyHein method note Record plaque index (plaque index, PLI), PLI score standard is as shown in table 1.
Table 1 PLI scores standard
Score Symptom
0 Facing is without bacterial plaque
1 The point-like bacterial plaque being dispersed in is had at neck portion gum edge
2 Neck portion bacterial plaque width is less than 1mm
3 Neck portion bacterial plaque width is less than 1mm, but at facing less than 1/3
4 Bacterial plaque area coverage accounts for facing 1/3 and 2/3 quality inspection
5 Bacterial plaque area coverage accounts for facing more than 2/3
2.2 experimental result
The specific experiment result of bacteriostatic experiment is as shown in table 2.
Table 2 bacteriostatic experiment result
Group Number PLI #P *P
Experimental group 10 1.93±0.78 ＜ 0.01 ＞ 0.05
Matched group 1 group 10 2.13±0.56 ＜ 0.01
Matched group 2 groups 10 3.86±1.01
Note: #P is to compare with matched group 2, and * P is to compare with matched group 1
As shown in Table 2, (1) compares with matched group 2 groups, and experimental group, matched group 1 group all can significantly press down Dental plaque processed is formed again, and has significant difference (P ＜ 0.01)；(2) experimental group and matched group 1 group Relatively, the effect that suppression dental plaque is formed again there was no significant difference.
Additionally, from the point of view of to indexs such as collutory satisfaction, oral mucosa zests, experimental group is commented Divide and be significantly higher than matched group 1 group；Each group does not all find untoward reaction.
3, experiment conclusion
From experimental example 1, the oral cavity gargle of embodiment 1 preparation can significantly inhibit dental plaque shape again Become, and toxic and side effects is less.This shows, the oral cavity gargle of the embodiment of the present invention 1 preparation has one Fixed bacteriostasis.
Experimental example 2The vitro cytotoxicity research of the present composition
1, experimental technique
1.1 laboratory sample
Using embodiment 3 preparation oral cavity gargle as test sample, with commercially available Li Shidelin (green tea Elite) collutory is reference substance 1, with commercially available Colgate-Palmolive (light sensation is white) collutory as reference substance 2, with commercially available Crest collutory for reference substance 3, it is right with commercially available black younger sister (mouth is safe) collutory According to product 4, it is reference substance 5 with commercially available black younger sister (fast clean) collutory, (clears away heart-fire with commercially available Black people Green tea) collutory is reference substance 6, with commercially available pure water for reference substance 7, with PBS as reference substance 8。
Above-mentioned reference substance microporous filter membrane is carried out filtration sterilization, standby.
1.2 experimental implementation
Being counted by blood cell counting plate, L929 cell is with 10 × 104The concentration of/mL is planted in 96 holes In plate, 100 μ L/ holes, 9 × 8 holes.2 piece of 96 orifice plate.
Cultivate after 48h, abandon old culture fluid, respectively with complete medium, reference substance 7/ reference substance 8, Test sample, reference substance 1, reference substance 2, reference substance 3, reference substance 4, reference substance 5, reference substance 6 After each 100 μ L/ holes process, discard sample immediately, and add complete medium 100 μ L/ hole cleaning 1 time. Finally, institute's porose addition 200 μ L/ hole complete medium, and continue to cultivate detection cell viability after 24h. Wherein, the sample processing time of plate A is 30S, and the sample processing time of plate B is 60S.
1.3 cell viability detections
Under dark condition, in each hole of plate A and plate B, add 20 μ L cell proliferation detectable, Continue to cultivate upper microplate reader detection after 2.5h, parameter be set as surveying front middling speed shake continuously 10min, in Quickly measure under 490nm.
2, experimental result
The specific experiment result of plate A as it is shown in figure 1, the specific experiment result of plate B as shown in Figure 1.
3, experiment conclusion
From Fig. 1 and Fig. 2, compared with other oral cavity gargles, the oral cavity of embodiment 3 preparation contains Gargarism is lower to the growth inhibition ratio of L929 cell, and toxicity is lower, and safety is higher, is more suitable for long Phase uses.
In sum, the present invention has antibacterial, hemostasis and the compositions of antiinflammation, is selected by optimization Selecting the proportioning of its composition and each component, each component cooperates according to its proportioning, jointly acts on, thus Play antibacterial, hemostasis and antiinflammation；And, compared with other oral cavity gargles, above-mentioned composition The vitro cytotoxicity of the oral cavity gargle made is lower, and safety is higher, is more suitable for life-time service.
Obviously, above-described embodiment is only for clearly demonstrating example, and not to embodiment party The restriction of formula.For those of ordinary skill in the field, the most also may be used To make other changes in different forms.Here without also all of embodiment being given With exhaustive.And the obvious change thus extended out or variation are still in the guarantor of the invention Protect among scope.

Claims (9)

1. one kind has antibacterial, hemostasis and the compositions of antiinflammation, it is characterised in that described group The raw material of compound includes:
Chitosan 2-10 weight portion, sorbitol 50-150 weight portion, glycerol 10-100 weight portion, dimension Raw element C 0.1-1.0 weight portion, potassium sorbate 0.05-0.50 weight portion, Bai Yaqilan extract 1-5 weight Amount part, pure water 100-500 weight portion.
The most according to claim 1 have antibacterial, hemostasis and the compositions of antiinflammation, its Being characterised by, the raw material of described compositions includes:
Chitosan 4-8 weight portion, sorbitol 60-100 weight portion, glycerol 20-60 weight portion, dimension are raw Element C 0.2-0.6 weight portion, potassium sorbate 0.10-0.20 weight portion, Bai Yaqilan extract 2-4 weight Part, pure water 120-300 weight portion.
The most according to claim 1 and 2 have antibacterial, hemostasis and the compositions of antiinflammation, It is characterized in that,
The raw material of described compositions also includes pigment, and described pigment is selected from sunset yellow, lemon yellow, light blue In at least one.
The most according to claim 3 have antibacterial, hemostasis and the compositions of antiinflammation, its It is characterised by,
Described sunset yellow is 0-0.4 weight portion, and described lemon yellow is 0-0.2 weight portion, and described light blue is 0-0.2 weight portion.
5. described in an any one of claim 1-4 have antibacterial, hemostasis and the combination of antiinflammation The preparation method of thing, it is characterised in that comprise the following steps:
(1) taking the chitosan of selected weight portion, adding appropriate volume percent is 0.2%～0.4% Acetic acid solution, is configured to the chitosan solution that percetage by weight is 0.5%～1.5%, and stirring is extremely dissolved, Adjusting pH is 5.0～6.0, obtains solution A, standby；
(2) take the pure water of selected weight portion, add vitamin C and the sorbic acid of selected weight portion Potassium, stirring, to dissolving, is then sequentially added into the sorbitol of selected weight portion, glycerol and Bai Ya under stirring Strange blue extract, stirs, obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH is 5.0～6.0, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
The system of the compositions with antibacterial, hemostasis and antiinflammation the most according to claim 5 Preparation Method, it is characterised in that comprise the following steps:
(1) take the chitosan of selected weight portion, add the acetic acid that appropriate volume percent is 0.3% molten Liquid, is configured to the chitosan solution that percetage by weight is 1.0%, and stirring is to dissolving, and adjusting pH is 5.5～5.7, obtain solution A, standby；
(2) take the pure water of selected weight portion, add vitamin C and the sorbic acid of selected weight portion Potassium, stirring, to dissolving, is then sequentially added into the sorbitol of selected weight portion, glycerol and Bai Ya under stirring Strange blue extract, stirs, obtains solution B, standby；
(3), under stirring, described solution A is slowly added to described solution B, stirs, Adjusting pH is 5.5～5.7, obtains solution C, standby；
(4) by described solution C first with filter paper filtering, again with 0.45 μm filtering with microporous membrane, sterilizing, Obtain.
7., hemostasis antibacterial according to having described in claim 5 or 6 and the compositions of antiinflammation Preparation method, it is characterised in that
In described step (2), adding vitamin C and the step of potassium sorbate of selected weight portion Before, also include the step adding the pigment of selected weight portion.
8. include described in any one of claim 1-4 have antibacterial, hemostasis and the combination of antiinflammation The preparation of thing or include the combination that the preparation method described in any one of claim 5-7 prepares The preparation of thing, it is characterised in that
Described compositions adds customary adjuvant, according to common process, makes the most acceptable liquid Preparation.
9. the compositions described in any one of claim 1-4, the system described in any one of claim 5-7 The preparation of the compositions described in compositions that Preparation Method prepares or claim 8 has in preparation Antibacterial, hemostasis and antiinflammation oral care product in application.
CN201610065041.8A 2016-01-29 2016-01-29 Composition with antibacterial function as well as preparation method and application thereof Pending CN105726783A (en)

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101627965A (en) * 2009-07-21 2010-01-20 清华大学 Multifunctional pure natural mouthwash and preparation method thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101627965A (en) * 2009-07-21 2010-01-20 清华大学 Multifunctional pure natural mouthwash and preparation method thereof

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