CN105724464B - A kind of biological pesticide and preparation method thereof - Google Patents
A kind of biological pesticide and preparation method thereof Download PDFInfo
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- CN105724464B CN105724464B CN201610056366.XA CN201610056366A CN105724464B CN 105724464 B CN105724464 B CN 105724464B CN 201610056366 A CN201610056366 A CN 201610056366A CN 105724464 B CN105724464 B CN 105724464B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- A01N65/385—Tobacco
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/48—Zingiberaceae [Ginger family], e.g. ginger or galangal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The present invention provides a kind of biological pesticides, in parts by weight, are made of following raw material：800 1200 parts of earthworm of work, 10 80 parts of ginger, 0.1 10 parts of chitosan, 150 900 parts of water.Preparation method includes the following steps：Smash ginger to pieces acquisition ginger juice；By Enzymatic Hydrolysis of Chitosan and obtain supernatant；Ginger juice and supernatant are mixed to get mixed liquor, then clean work earthworm is put into mixed liquor, 1 is placed at a temperature of 25 40 DEG C, filtrate is obtained by filtration afterwards for 24 hours, it is biological pesticide to boil after enzyme deactivation.The biological pesticide of the present invention can be used in the prevention and control of plant diseases, pest control of various crop, and be readily biodegradable.
Description
Technical field
The present invention relates to pesticide fields, in particular to a kind of biological pesticide and preparation method thereof.
Background technology
Widely used chemical pesticide in agricultural production, not only causes environmental pollution, but also causes agricultural product middle peasant
Medicine is remaining exceeded.Due to the carcinogenic of pesticide, teratogenesis, mutagenic " three cause " effect, remains of pesticide to humans and animals especially
The harm of the top of food chain -- people is maximum, has seriously affected health and the foreign exchange earning of people.As people live
The enhancing of horizontal raising and environmental consciousness, there is an urgent need to organic pollution-free agricultural product of non agricultural chemical residuum by people.But in agricultural
If will inevitably cause crop production reduction due to the invasion of pest and disease damage without using chemical pesticide in production, this is to that will solve
It is certainly unpractical for the food shortage problem of facing mankind.Most effective hand of the chemical pesticide as current disease and pest control
Section will also continue a large amount of use.
Exploitation is easy to the biological pesticide substituting chemical pesticide of degradation, is to promote Organic Farming, reduces pesticide residue, protects
Hinder effective way of the people to healthy food demand.
The biological pesticide of the prior art, antibacterial insecticidal active ingredient content is low, and insecticidal and antibacterial ability is weak, is applied to crop disease
Pest prevention is limited.Need fiting chemical Pesticide use, ecological environment certainly will be influenced, eventually lead to agriculture remain it is tired.
Invention content
The first object of the present invention is to provide a kind of biological pesticide, and the biological pesticide not only has insecticidal and antibacterial energy
Power, and have many advantages, such as that the activity of coordinate plant growth, raw material be pure natural, non agricultural chemical residuum.
The above-mentioned purpose of the present invention, spy use following technical scheme：
A kind of biological pesticide is made of following raw material in parts by weight：Live 800-1200 parts of earthworm, ginger 10-80
Part, 0.1-10 parts of chitosan, 150-900 parts of water.
The second object of the present invention is to provide a kind of preparation method of biological pesticide, include the following steps：
It smashs ginger to pieces acquisition ginger juice, chitosan plus water is digested and obtained supernatant, ginger juice and supernatant are mixed
Conjunction obtains mixed liquor, and then clean work earthworm is put into mixed liquor, and mistake after 1-24h is placed under 25-40 DEG C of temperature condition
Filter obtains filtrate, will be biological pesticide after the filtrate enzyme deactivation.
The biological pesticide obtained by this law has the function of stronger antibacterial and promotion plant growth, adaptable, application
Range is wide, it can not only be widely used in the prevention and control of plant diseases, pest control of various crop, complete substituting chemical pesticide, while can improve comprehensively
The drought resisting of crop, cold-resistant, the ability of disease and insect resistance have the function of promoting plant growth, realize stable high yield, make the agriculture of production
Product reaches organic farm products requirement.
Importantly, the antibacterial increasing agent belongs to pure natural source, it is readily biodegradable, moreover it is possible to promote soil and plant
The degradation of middle harmful components is used for a long time, can improve agroecological environment.
The pest and disease damage that the biological pesticide of the present invention can be used for the multiple kinds of crops such as veterinary antibiotics, rice, corn, wheat is anti-
It controls, increases yield, stable high yield.
Specific implementation mode
Chitosan (chitosan) is the product after chitin is deacetylated, is a kind of cationic polysaccharide, and at present
Unique commercialization alkaline polysaccharide.Chitosan is a kind of macromolecule cation flocculant, due to it is nontoxic, can by biology drop
The good characteristics such as solution, good biocompatibility and film forming.
The body fluid of earthworm living is rich in antibacterial peptide and up to 18 kinds or more of free amino acid, can either antibacterial can give again
Plant provides nutrition.
Preferably, in parts by weight, live 1000-1200 parts of earthworm, 50-80 parts of ginger, 1-10 parts of chitosan, water
150-900 parts.
Preferably, 1000-1200 parts of earthworm living, 50-80 parts of ginger, 5-10 parts of chitosan, 150-900 parts of water.
Preferably, the deacetylation of the chitosan is more than 70%.
Ginger contains the substances such as gingerol, flavonoids and terpenes, has anti-oxidant and antibacterial action, can stimulate earthworm
Spit liquid.
Become the chitosan oligosaccharide containing 2-10 aminoglucose saccharide residue after Enzymatic Hydrolysis of Chitosan, there is immunological enhancement, suppression
Bacterium acts on, and can also increase product storage stability.
Contain fibrinolysin, the excessively high extraction that earthworm can be made to be unfavorable for earthworm antimicrobial fluid of temperature in earthworm body living.
Preferably, earthworm living is put into mixed liquor, filtering enzyme deactivation obtains after placement 5-24h under 35-37 DEG C of temperature condition
To the earthworm antimicrobial fluid with physiological activity.
Preferably, the catalyst using complex enzyme as Enzymatic Hydrolysis of Chitosan, complex enzyme is by cellulase protein enzyme and fat
Fat enzyme.
Enzymolysis is specially：Chitosan, complex enzyme and water are mixed to get chitosan mixed liquor, chitosan mixed liquor is stirred
0.5-2h is placed in constant temperature oscillator, and 5-24h is reacted under 30-65 DEG C of constant temperature, then adds chitosan mixed liquor
Heat inactivates complex enzyme to 95-100 DEG C, then filters off complex enzyme and obtains supernatant.
Preferably, the weight of chitosan is the 10-50% of the weight of solvent, the weight of complex enzyme is the weight of chitosan
1-10%.
Preferably, the weight of chitosan is the 10-20% of the weight of solvent, the weight of complex enzyme is the weight of chitosan
5-15%.
Preferably, the weight ratio of cellulase, protease and lipase is 5-10:2-4:2-4.
Preferably, the deacetylation of chitosan is more than 70%.
As to the further preferred of biological pesticide, a kind of biological pesticide is made of following raw material in parts by weight：
In parts by weight, 1000-1200 parts of earthworm living, 50-80 parts of ginger, 1-10 parts of chitosan, 1-10 parts of tobacco leaf, water 150-900
Part.
For tobacco leaf containing Salanesol, nicotine, aldoketones, alcohols, terpenes, nitrogen heterocycles etc. more than 20 plant active ingredient, have compared with
Strong killing pests and suppressing bacteria effect.Fresh tobacco leaves, which may be used, in tobacco leaf can also use waste tobacco leaf.
The processing method of tobacco leaf is：Tobacco leaf is added to the water progress broken wall treatment and obtains tobacco leaf mixture, tobacco leaf is mixed
Object obtains leaf tobacco extract after being separated by solid-liquid separation, leaf tobacco extract and filtrate are then mixed and made into biological pesticide.
The broken wall treatment of tobacco leaf, mechanical breaking-wall method, which may be used, can also use enzymolysis broken wall.If selecting enzymolysis broken wall, need
Select cellulase, pectase and lignoenzyme.
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
Raw material：1000 parts of earthworm of work, 50 parts of ginger, 10 parts of chitosan, 150 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 0.5h is placed on
In constant temperature oscillator, 12h is reacted under 50 DEG C of constant temperature, and chitosan mixed liquor is then heated to 100 DEG C to complex enzyme
It is inactivated, then filter off complex enzyme and obtains supernatant；Wherein 0.5 part of complex enzyme cellulase .2 parts of proteinase-10 and fat
0.2 part of fat enzyme；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 12h under 30 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Embodiment 2
Raw material：1200 parts of earthworm of work, 55 parts of ginger, 9 parts of chitosan, 200 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes 100 parts of 0.1 part of complex enzyme, 1 part of chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor is stirred
It mixes 0.5h to be placed in constant temperature oscillator, 10h is reacted under 52 DEG C of constant temperature, be then heated to chitosan mixed liquor
100 DEG C inactivate complex enzyme, then filter off complex enzyme and obtain supernatant；Wherein 0.35 part of complex enzyme cellulase,
0.15 part of .2 parts of proteinase-10 and lipase；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 8h under 35 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Embodiment 3
Raw material：1400 parts of earthworm of work, 60 parts of ginger, 8 parts of chitosan, 300 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 1h is placed on perseverance
In warm oscillator, 6h is reacted under 54 DEG C of constant temperature, and chitosan mixed liquor is then heated to 95-100 DEG C to complex enzyme
It is inactivated, then filter off complex enzyme and obtains supernatant；Wherein 0.3 part of complex enzyme cellulase .15 parts of proteinase-10 and
0.1 part of lipase；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 20h under 28 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 10 minutes,
Liquid cooling to be filtered is but biological pesticide afterwards.
Embodiment 4
Raw material：1600 parts of earthworm of work, 65 parts of ginger, 7 parts of chitosan, 400 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 2h is placed on perseverance
In warm oscillator, 5h is reacted under 55 DEG C of constant temperature, chitosan mixed liquor, which is then heated to 100 DEG C, carries out complex enzyme
Then inactivation filters off complex enzyme and obtains supernatant；Wherein 0.2 part of complex enzyme cellulase, 0.1 part of fructose enzyme and lignin
0.06 part of enzyme；
Ginger juice and supernatant are mixed to get mixed liquor, then clean work earthworm is put into mixed liquor, at 30 DEG C
Filtrate is obtained by filtration after placing 12h under temperature condition；
Tobacco leaf extraction step：Tobacco leaf is added to the water and carries out broken wall treatment with cellulase and obtains tobacco leaf mixture, it will
Tobacco leaf mixture obtains leaf tobacco extract after being separated by solid-liquid separation；
Mixed preparing step：The filtrate sterilize after imperial liquid is mixed with leaf tobacco extract, being obtained by filtration is biological pesticide.
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 5h under 36 DEG C of temperature conditions；
Tobacco leaf is added to the water and carries out broken wall treatment with cellulase and obtains tobacco leaf mixture, tobacco leaf mixture is carried out
Leaf tobacco extract is obtained after separation of solid and liquid；
Leaf tobacco extract is added in filtrate and is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, liquid cooling to be filtered is but afterwards
Biological pesticide.
Embodiment 5
Raw material：1125 parts of earthworm of work, 70 parts of ginger, 5 parts of chitosan, 2500 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 1h is placed on perseverance
In warm oscillator, is reacted under 50 DEG C of constant temperature for 24 hours, chitosan mixed liquor is then heated to 95-100 DEG C to complex enzyme
It is inactivated, then filter off complex enzyme and obtains supernatant；Wherein 0.1 part of complex enzyme cellulase, proteinase-10 .06 and fat
0.04 part of fat enzyme enzyme；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 5h under 36 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Embodiment 6
Raw material：1150 parts of earthworm of work, 72 parts of ginger, 4 parts of chitosan, 600 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 1h is placed on perseverance
In warm oscillator, 8h is reacted under 54 DEG C of constant temperature, chitosan mixed liquor, which is then heated to 95 DEG C, carries out complex enzyme
Then inactivation filters off complex enzyme and obtains supernatant；Wherein 0.08 part of complex enzyme cellulase .05 parts of proteinase-10 and fat
0.03 part of enzyme；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 2h under 40 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Embodiment 7
Raw material：1750 parts of earthworm of work, 75 parts of ginger, 2 parts of chitosan, 800 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 1h is placed on perseverance
In warm oscillator, 10h is reacted under 54 DEG C of constant temperature, chitosan mixed liquor, which is then heated to 98 DEG C, carries out complex enzyme
Then inactivation filters off complex enzyme and obtains supernatant；Wherein 0.06 part of complex enzyme cellulase .04 parts of proteinase-10 and fat
0.03 part of enzyme；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 1h under 40 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Embodiment 8
Raw material：1200 parts of earthworm of work, 80 parts of ginger, 1 part of chitosan, 900 parts of water.
A kind of preparation method of biological pesticide, includes the following steps：
It takes complex enzyme, chitosan and water to be mixed to get chitosan mixed liquor, chitosan mixed liquor stirring 2h is placed on perseverance
In warm oscillator, 5h is reacted under 55 DEG C of constant temperature, chitosan mixed liquor, which is then heated to 100 DEG C, carries out complex enzyme
Then inactivation filters off complex enzyme and obtains supernatant；Wherein 0.04 part of complex enzyme cellulase .03 parts of proteinase-10 and fat
0.03 part of enzyme；
Ginger crush taking juice and supernatant are mixed to get mixed liquor, clean work earthworm is then put into mixed liquor
In, filtrate is obtained by filtration after placing 12h under 30 DEG C of temperature conditions, filtrate is heated to 95-100 DEG C of holding enzyme deactivation in 5 minutes, is waited for
It is biological pesticide after filtrate cooling.
Test example 1
Enough biological pesticides are prepared using the method for embodiment 8, by this biology with being administered demonstration on rice.
The place of demonstration area：The rice field of the towns the Ding Jia villages San Wu two society and four societies amounts to 104 mu；Height above sea level is 290 meters,
It as late autumn is Rapeseed Field that soil, which is upper, preceding in medium loam, fertility,.
Subjects：Selenium-rich perfume (or spice) rice.
In this experiment, demonstration area is divided into two regions, the two regions are carried out at the same time two groups of experiments.Second group is applied
With the biological pesticide of the present invention, first group is applied clear water as blank control.
First group：March 2, with Seed soaking 48 hours；5-March 6 March is sowed, using mulch water-raised seedling or drought
Seedling；April, 25-April 29, transplanted rice shoot, and root dipping is carried out with clear water before transplanting, and 3 plants of paddy seedlings are planted per nest, are planted per acre
8000 nests, line-spacing 30cm, nest spacing 28cm.Use pure N 10kg, KCl 10kg, calcium superphosphate 40kg as fertilizer after transplanting,
Wherein 60% nitrogenous fertilizer as base fertilizer, 30% make nitrogenous fertilizer be tillering fertilizer and after transplanting 10 days apply, half potash fertilizer be used as base fertilizer it is another
Half potash fertilizer is used as jointing fertilizer, and phosphate fertilizer makees base fertilizer application entirely.20-25 days and concentration clear water on 17-May of May 18 after transplanting
The page for carrying out rice shoot sprays.
Second group：March 2, with 8 this biological pesticide 1 of the embodiment of the present invention:30 times of liquid are soaked seed 48 hours；On March 5-3
The moon 6 was sowed, using mulch water-raised seedling or dry rice seedling；April, 25-April 29, transplanted rice shoot, with 1 before transplanting:
30 times of biological pesticide carries out root dipping, and 3 plants of paddy seedlings are planted per nest, plant 8000 nests, line-spacing 30cm, nest spacing 28cm per acre.Transplanting terminates
Use the chicken manure in chicken farm as fertilizer afterwards.20-25 days and 17-May of May 18 concentrate with 1 after transplanting:30 times of biological pesticide
The page for carrying out rice shoot sprays.
Output condition compares：
August 11 days carries out random sampling to first group and second group of selenium-rich perfume (or spice) rice and surveys production.Field receives at 3 points at random, often
100 nests of point, total 56 jin of paid paddy, it is 3.9 jin to weigh wherein 5 jin of paddy and dry rear dry weight, according to the converting standard of the Ministry of Agriculture
(conversion factor 0.85), and carry and plant specification for 0.8 ten thousand nests/mu calculating.
The yield of first group of survey is 734.6 jin, and second group of yield measured is 928.2 jin, second group of than first group fecund
193.6 jin, that is to say, that increase production 26.4% after applied the biological pesticide of the present invention.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (9)
1. a kind of biological pesticide, which is characterized in that in parts by weight, be mainly made of following raw material：Earthworm 800-1200 living
Part, 10-80 parts of ginger, 0.1-10 parts of chitosan, 150-900 parts of water；
The preparation method of the biological pesticide includes the following steps：
Smash ginger to pieces acquisition ginger juice；
Catalyst using complex enzyme as Enzymatic Hydrolysis of Chitosan, the complex enzyme are made of cellulase, protease and lipase；
The chitosan, the complex enzyme and water are mixed to get chitosan mixed liquor；The chitosan mixed liquor is stirred into 0.5-2h
It is placed in constant temperature oscillator, 5-24h is reacted under 30-65 DEG C of constant temperature, then heat the chitosan mixed liquor
The complex enzyme is inactivated to 95-100 DEG C, then filter off the complex enzyme and obtains the supernatant；
The ginger juice and the supernatant are mixed to get mixed liquor, clean work earthworm is then put into the mixed liquor
In, filtrate is obtained by filtration after placing 1-24h under 25-40 DEG C of temperature condition, will be biological pesticide after the filtrate enzyme deactivation.
2. biological pesticide according to claim 1, which is characterized in that in parts by weight, the earthworm 1000-1200 living
Part, it is ginger 50-80 parts described, chitosan 1-10 parts described, water 150-900 parts described.
3. biological pesticide according to claim 1, which is characterized in that the earthworm 1000-1200 parts living, ginger
50-80 parts, it is chitosan 5-10 parts described, water 150-900 parts described.
4. a kind of preparation method of biological pesticide as described in any one of claims 1-3, which is characterized in that including following step
Suddenly：
Smash ginger to pieces acquisition ginger juice,
Catalyst using complex enzyme as Enzymatic Hydrolysis of Chitosan, the complex enzyme are made of cellulase, protease and lipase；
Chitosan plus water are digested and obtained supernatant, chitosan plus water are digested and obtained supernatant, the enzymolysis is specially：
The chitosan, the complex enzyme and water are mixed to get chitosan mixed liquor, the chitosan mixed liquor is stirred into 0.5-2h
It is placed in constant temperature oscillator, 5-24h is reacted under 30-65 DEG C of constant temperature, then heat the chitosan mixed liquor
The complex enzyme is inactivated to 95-100 DEG C, then filter off the complex enzyme and obtains the supernatant；
The ginger juice and the supernatant are mixed to get mixed liquor, clean work earthworm is then put into the mixed liquor
In, filtrate is obtained by filtration after placing 1-24h under 25-40 DEG C of temperature condition, will be biological pesticide after the filtrate enzyme deactivation.
5. the preparation method of biological pesticide according to claim 4, which is characterized in that the weight of the chitosan is water
The 10-50% of weight, the weight of the complex enzyme are the 0.5-15% of the weight of the chitosan.
6. the preparation method of biological pesticide according to claim 4, which is characterized in that the weight of the chitosan is water
The 10-20% of weight, the weight of the complex enzyme are the 5-10% of the weight of the chitosan.
7. the preparation method of biological pesticide according to claim 4, which is characterized in that the cellulase, the albumen
The weight ratio of enzyme and the lipase is 5-10:2-4:2-4.
8. the preparation method of biological pesticide according to claim 4, which is characterized in that the deacetylation of the chitosan is big
In 70%.
9. according to the preparation method of claim 4-8 any one of them biological pesticides, which is characterized in that the raw material further includes
The tobacco leaf broken wall treatment is obtained leaf tobacco extract, then the leaf tobacco extract and the filtrate is mixed with life by tobacco leaf
Object pesticide.
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| CN1721373A (en) * | 2004-07-12 | 2006-01-18 | 湖南长沙金壳生物技术有限公司 | Preparation and process for ecological environmental-protection fertilizer |
| CN1802930A (en) * | 2005-12-22 | 2006-07-19 | 重庆市璧山生物工程研究所 | Active biological degradation agent for increasing production |
| CN101731220A (en) * | 2009-12-25 | 2010-06-16 | 浙江大学 | Application of chitosan in control of plant bacterial wilt |
| CN102206103A (en) * | 2010-03-30 | 2011-10-05 | 陈金明 | Active biological nutrient solution, and manufacturing method and purpose thereof |
| CN103190451A (en) * | 2013-04-07 | 2013-07-10 | 海南正业中农高科股份有限公司 | Oligochitosan and plant source substance-containing agricultural composition and applications |
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| WO1998011034A1 (en) * | 1996-09-11 | 1998-03-19 | Farhad Karim | Compositions containing earthworm excrements and/or extracts thereof and their use for plant cultures |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1721373A (en) * | 2004-07-12 | 2006-01-18 | 湖南长沙金壳生物技术有限公司 | Preparation and process for ecological environmental-protection fertilizer |
| CN1802930A (en) * | 2005-12-22 | 2006-07-19 | 重庆市璧山生物工程研究所 | Active biological degradation agent for increasing production |
| CN101731220A (en) * | 2009-12-25 | 2010-06-16 | 浙江大学 | Application of chitosan in control of plant bacterial wilt |
| CN102206103A (en) * | 2010-03-30 | 2011-10-05 | 陈金明 | Active biological nutrient solution, and manufacturing method and purpose thereof |
| CN103190451A (en) * | 2013-04-07 | 2013-07-10 | 海南正业中农高科股份有限公司 | Oligochitosan and plant source substance-containing agricultural composition and applications |
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