CN105709278B - Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion - Google Patents
Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion Download PDFInfo
- Publication number
- CN105709278B CN105709278B CN201510224166.6A CN201510224166A CN105709278B CN 105709278 B CN105709278 B CN 105709278B CN 201510224166 A CN201510224166 A CN 201510224166A CN 105709278 B CN105709278 B CN 105709278B
- Authority
- CN
- China
- Prior art keywords
- spinal cord
- nerve cell
- aixs cylinder
- area
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000000278 spinal cord Anatomy 0.000 title claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 34
- 210000002569 neuron Anatomy 0.000 claims abstract description 33
- 230000004009 axon guidance Effects 0.000 claims abstract description 10
- 239000002356 single layer Substances 0.000 claims abstract description 5
- 210000004027 cell Anatomy 0.000 claims description 15
- 210000004885 white matter Anatomy 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- 210000004884 grey matter Anatomy 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims 2
- 230000001174 ascending effect Effects 0.000 claims 1
- 230000008520 organization Effects 0.000 claims 1
- 210000005036 nerve Anatomy 0.000 abstract description 10
- 230000037361 pathway Effects 0.000 abstract description 4
- 230000010354 integration Effects 0.000 abstract description 2
- 238000011084 recovery Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract 2
- 210000001519 tissue Anatomy 0.000 description 8
- 208000020431 spinal cord injury Diseases 0.000 description 7
- 210000003050 axon Anatomy 0.000 description 5
- 238000004088 simulation Methods 0.000 description 5
- 239000010410 layer Substances 0.000 description 4
- 108010082117 matrigel Proteins 0.000 description 3
- 210000005056 cell body Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 1
- 108090000368 Fibroblast growth factor 8 Proteins 0.000 description 1
- 102100037680 Fibroblast growth factor 8 Human genes 0.000 description 1
- 102000009065 Netrin-1 Human genes 0.000 description 1
- 108010074223 Netrin-1 Proteins 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000013528 artificial neural network Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000002161 motor neuron Anatomy 0.000 description 1
- 230000007433 nerve pathway Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 210000002804 pyramidal tract Anatomy 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
Abstract
The tissue engineering spinal cord of orientation aixs cylinder is contained in a kind of subregion, the direction of the issued aixs cylinder of nerve cell in timbering material can be controlled by region, and can greatly reduce nerve cell in timbering material and change first number.For nerve cell in uplink area in monolayer distribution in the bottom of timbering material, the substance for being adsorbed with axon guidance cue is placed in the top of timbering material；For nerve cell in descender in monolayer distribution in the top of timbering material, the substance for being adsorbed with axon guidance cue is placed in the bottom of conduit.Through cultivating, the nerve cell in uplink area can grow aixs cylinder upwards, and the nerve cell of descender can grow downwards aixs cylinder.Therefore the present invention can simulate the directionality of physiological status Nervous pathway, and change the connection reparation that first number completes cross-section spinal cord with less neuron.To guarantee the tissue engineering spinal cord and the stability and accuracy of nerve signal transmitting after the integration of host's spinal cord of transplanting, promote the recovery of spinal function.
Description
Technical field:
The present invention relates to a kind of for repairing the tissue engineering spinal cord of spinal cord injury, especially contains according to different zones
Capable or descending axons tissue engineering spinal cords.
Background technique:
Nerve cell/neuron belongs to permanent cell, lacks power of regeneration, therefore spinal cord injury, especially spinal cord are horizontal
The patient of disconnected wound is difficult to voluntarily restore normal spinal function.In spinal cord injury repairing research field, it is artificial that people attempt building
Spinal cord rack material and composite nerve trophic factors and seed cell repair spinal cord injury, the basic principle is that: the people of transplanting
Work timbering material can repair relevant cell for spinal cord and provide suitable growing environment, and seed cell in timbering material is promoted (to close
Key is nerve cell) Growth and Differentiation and host's spinal cord broken ends of fractured bone neuron axon regeneration, with the effect of similar relay station by place
Neural network reconnects main lesion level up and down.
Neuron is a kind of polarized cell, mainly by cell space (including dendron) and the root long issued by cell space in form
Aixs cylinder composition.The Nervous pathway in spinal cord, which will be undergone, under normal circumstances once changes member to neuron for several times, upper when changing member
The aixs cylinder of neuron is connected with the cell space of lower motor neuron or dendron, the neuron axon after changing member and changes the neuron axis before member
It dashes forward and is consistent on direction.
However, all kinds of patents and document report that related man-made support material is repaired with spinal cord injury are consulted, we
It was found that existing timbering material even partition type tissue engineering spinal cord is also only in the distributed areas of uplink and downlink nerve fibre bundle
Provided with longitudinal duct, and it is unable to control the direction of the issued aixs cylinder of nerve cell in conduit, i.e., the nerve in longitudinal duct is thin
Born of the same parents can be random upward or downward grow aixs cylinder.The aixs cylinder grown up as nerve cell is issued in conduit may be with host
Downlink nerve cell axons are connected, and this connection type cannot exercise its normal function.And due to the nerve of plantation
Cell is uniformly distributed in entire three-dimensional bracket material, and it is multiple in timbering material that this will lead to uplink and downlink Nervous pathway
It changes member and aixs cylinder directionality erroneous matching occurs, prevent nerve signal from effectively conducting, repairing effect is had a greatly reduced quality.
Summary of the invention:
In order to overcome randomness and nerve cell of the nerve cell axons in the direction of growth in existing tissue engineering spinal cord
The excessive deficiency of first number is changed, the present invention provides a kind of novel tissue engineering spinal cords, which can be by region
The direction of the issued aixs cylinder of nerve cell is controlled, and nerve cell in material can be greatly reduced and change first number.
The technical solution adopted by the present invention to solve the technical problems is: timbering material is marked off according to aixs cylinder trend
Row region and descender region.Nerve cell will be planted in the upper end of timbering material, the nerve in aixs cylinder uplink area in descender
Cell will be planted in the lower end of timbering material.We can be placed in sustained release aixs cylinder in the material other end opposite with cell and lead simultaneously
To the material of the factor (such as FGF-2, FGF-8, netrin-1 etc.), aixs cylinder that inducing nerve cell issues towards axon guidance because
Direction growth where sub.In vitro culture for a period of time, the axis of certain length is grown to nerve cell in timbering material interior orientation
After prominent, this timbering material containing orientation aixs cylinder is transplanted in the organism of spinal cord injury by we again.When host's nerve is thin
Born of the same parents' aixs cylinder is grown into our timbering material, without undergoing the growth of long range can be with the nerve positioned at material upper end or lower end
Cell body or dendron establish connection, and the nerve cell after member is changed in material can be with same tropism aixs cylinder and host junior Neuronal soma
Or dendron establishes connection.Thus we can reach and repair effectively by uplink and downlink nerve pathway according to the direction connection again of script
The purpose of multiple spinal cord injury.
The beneficial effects of the present invention are: the directionality of simulation physiological status Nervous pathway, and it is thin with less nerve
Born of the same parents change the connection reparation that first number completes cross-section spinal cord.Tissue engineering material to guarantee transplanting and mind after the integration of host's spinal cord
The stability and accuracy transmitted through signal, promote the recovery of spinal function.
Detailed description of the invention:
Present invention will be further explained below with reference to the attached drawings and examples.
Fig. 1 is single longitudinal duct and its internal structure ideograph of the invention.
Fig. 2 is the cross section ideograph of one embodiment of the present of invention.
Fig. 3 is the I--I cross-sectional view of Fig. 2.
Specific embodiment:
In Fig. 1, nerve cell (1) is planted in one end of conduit (4), and the material (3) containing axon guidance cue exists
The other end in conduit (4), in vitro in incubation, the aixs cylinder (2) issued by cell space (1) can be deposited towards axon guidance cue
Direction oriented growth.To guarantee that axon guidance cue forms the concentration gradient successively decreased to cell space end in conduit, contain aixs cylinder
The material (3) of guidance cues is preferably selected slow-release material (we select gelfoam in the present embodiment).
In the embodiment depicted in figure 2, tissue engineering spinal cord timbering material marks the model of grey matter according to corresponding spinal segment
It encloses (9), and white matter part includes that the big section of uplink and downlink two, wherein uplink area and descender can be as required by further thin
Point, in the present embodiment, uplink area is further divided into rear uplink area (5) and the simulation spinal cord of simulation fasciculus gracilis cuneate fascicle by we
The side uplink area (6) of fasciculus thalamicus, and descender is further divided into preceding descender (8) and the simulation of simulation direct pyramidal tract
The side descender (8) of tractus corticospinalis lateralis.
Embodiment shown in Fig. 3 illustrates the I--I cross-sectional view of Fig. 2, includes to be permitted in rear uplink area (5) and preceding descender (8)
More longitudinal ducts (16), nerve cell is laid on the spawn (matrigel) of thin layer first and cultivates by we, to thin
After born of the same parents' attaching is stablized, the matrigel layer (12) containing monolayer neuronal cell is gently removed, covering is packed in rear uplink area (5)
Bottom, while gelfoam floor (10) covering containing axon guidance cue is packed in the top of rear uplink area (5).And
Preceding descender (8), matrigel layer (13) covering containing monolayer neuronal cell are packed in its top, contain axon guidance cue
Gelfoam layer (11) covering be packed in its bottom.Timbering material is laterally statically placed in culture a period of time (4-7 in culture solution
It), the nerve cell for being located at rear uplink area (5) bottom is grown aixs cylinder (14) upwards, and the nerve cell at the top of preceding descender (8)
Aixs cylinder is grown downwards.
Claims (2)
1. a kind of subregional organization's engineering spinal cord, by bioavailability bracket material, cell factor and is planted in timbering material
Seed cell constitute, and white matter area and grey matter area are divided into, according to Ascending Fiber beam and Descending fibers beam in white matter area
Longitudinal duct is arranged in form range；It is characterized in that: white matter area part includes uplink area and the descender section Liang great, uplink area is under
Comprising the orientation aixs cylinder issued by nerve cell in row area longitudinal duct, the nerve cell in uplink area concentrates on the bottom of conduit
Portion, the material for being adsorbed with axon guidance cue are placed in the top of conduit, and the nerve cell of descender concentrates on the top of conduit, inhale
Material with axon guidance cue is placed in the bottom of conduit.
2. tissue engineering spinal cord according to claim 1, it is characterized in that the nerve cell in material uplink and downlink area is in single layer
Arrangement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510224166.6A CN105709278B (en) | 2015-05-06 | 2015-05-06 | Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510224166.6A CN105709278B (en) | 2015-05-06 | 2015-05-06 | Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105709278A CN105709278A (en) | 2016-06-29 |
| CN105709278B true CN105709278B (en) | 2019-05-21 |
Family
ID=56144700
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510224166.6A Active CN105709278B (en) | 2015-05-06 | 2015-05-06 | Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105709278B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278865A (en) * | 2008-05-09 | 2008-10-08 | 南通大学 | Partition type tissue engineering spinal cord |
| CN101766836A (en) * | 2009-01-21 | 2010-07-07 | 丁坦 | Preparation method of nano silver cordspinal cord and peripheral nerve repairing material |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008103744A1 (en) * | 2007-02-20 | 2008-08-28 | Rutgers, The State University Of New Jersey | Nerve guidance tubes |
-
2015
- 2015-05-06 CN CN201510224166.6A patent/CN105709278B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101278865A (en) * | 2008-05-09 | 2008-10-08 | 南通大学 | Partition type tissue engineering spinal cord |
| CN101766836A (en) * | 2009-01-21 | 2010-07-07 | 丁坦 | Preparation method of nano silver cordspinal cord and peripheral nerve repairing material |
Non-Patent Citations (2)
| Title |
|---|
| "Astrocyte infiltration into injectable collagen-based hydrogels containing FGF-2 to treat spinal cord injury";Daniel J. Macaya等;《Biomaterials》;20130213;第34卷(第14期);3591-3602 |
| "组织工程支架及其修复技术在脊髓损伤中的应用";邵阳 等;《中国组织工程研究与临床康复》;20091217;第13卷(第51期);10129-10132 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105709278A (en) | 2016-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hodos et al. | Visual intensity and pattern discrimination deficits after lesions of ectostriatum in pigeons | |
| US20210393960A1 (en) | Implantable Living Electrodes And Methods For Use Thereof | |
| Purves | Body and brain: a trophic theory of neural connections | |
| Hodgson et al. | Training-induced and electrically induced potentiation in the neocortex | |
| Li et al. | Peripheral nerve regeneration with 3D printed bionic scaffolds loading neural crest stem cell derived Schwann cell progenitors | |
| JP2013518591A (en) | Manufacture of modified biological nerve grafts and uses thereof | |
| CN104178422B (en) | A kind of neural axon tractive grower | |
| Morris et al. | The embryonic development of the flatworm Macrostomum sp. | |
| CN109496936A (en) | A kind of cray stereoscopic cultivation method | |
| Monfils et al. | Basic fibroblast growth factor stimulates functional recovery after neonatal lesions of motor cortex in rats | |
| Stewart et al. | Machine intelligence for nerve conduit design and production | |
| Dursun Usal et al. | Fabrication of a 3D printed PCL nerve guide: In vitro and in vivo testing | |
| CN101278865B (en) | Partition type tissue engineering spinal cord | |
| CN105709278B (en) | Contain the tissue engineering spinal cord of orientation aixs cylinder in subregion | |
| US20210236696A1 (en) | Artificial nerve conduit construction using tissue engineering methods | |
| CN110507857A (en) | A kind of engineered nerve graft and preparation method thereof | |
| de Siqueira-Santos et al. | Biological activity of laminin/polylaminin-coated poly-ℇ-caprolactone filaments on the regeneration and tissue replacement of the rat sciatic nerve | |
| CN204058481U (en) | A kind of neural axon tractive growing apparatus | |
| Aisaka et al. | Two modes of corticospinal reinnervation occur close to spinal targets following unilateral lesion of the motor cortex in neonatal hamsters | |
| Sievert | An anatomical, electrophysiological, and behavioral comparison of the primary and supplementary motor areas of the rat | |
| Mundiñano et al. | Somatosensory cross-modal plasticity in the superior colliculus of visually deafferented rats | |
| CN114945391A (en) | 3D bioprinting scaffold for tissue regeneration | |
| Dryg | Modulating Neuroinflammation with Porous Templated Scaffolds | |
| Caprile et al. | Analyzing the role of extracellular matrix during nervous system development to advance new regenerative strategies | |
| Li et al. | Models for evaluating glioblastoma invasion along white matter tracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Zhou Han Document name: Notification of Passing Preliminary Examination of the Application for Invention |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhou Han Document name: Notification of Publication and of Entering the Substantive Examination Stage of the Application for Invention |
|
| DD01 | Delivery of document by public notice |
Addressee: Zhou Han Document name: Notification that Application Deemed to be Withdrawn |
|
| DD01 | Delivery of document by public notice | ||
| DD01 | Delivery of document by public notice |
Addressee: Zhou Han Document name: Notification of Passing Examination on Formalities |
|
| DD01 | Delivery of document by public notice | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190408 Address after: 20101 No. 2880 Qiju Road, Minhang District, Shanghai Applicant after: Shanghai Blue Cross Brain Hospital Co., Ltd. Address before: 45 000 1 Liuyuan 15, No. 100 Science Avenue, Zhengzhou High-tech Zone, Henan Province Applicant before: Zhou Han |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200615 Address after: Room 158, floor 14, building 2, No. 588, Zixing Road, Minhang District, Shanghai 200000 Patentee after: Shanghai Lansheng brain medical research Co., Ltd Address before: 20101 No. 2880 Qiju Road, Minhang District, Shanghai Patentee before: Shanghai Blue Cross Brain Hospital Co.,Ltd. |
|
| TR01 | Transfer of patent right |